Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1076260 | AES78040.1 | 91.803 | 183 | 13 | 1 | 5 | 185 | 1 | 183 | 7.63e-119 | 344 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1076260 | sp|O81788|WOX13_ARATH | 53.289 | 152 | 49 | 3 | 21 | 150 | 33 | 184 | 6.95e-39 | 136 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1076260 | G7KVS3 | 91.803 | 183 | 13 | 1 | 5 | 185 | 1 | 183 | 3.64e-119 | 344 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1076260 | TF | HB-WOX |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1076260 | MtrunA17_Chr7g0222981 | 91.803 | 183 | 13 | 1 | 5 | 185 | 1 | 183 | 7.02e-123 | 344 |
| Msa1076260 | MtrunA17_Chr1g0212511 | 56.989 | 186 | 56 | 6 | 5 | 167 | 5 | 189 | 3.21e-59 | 186 |
| Msa1076260 | MtrunA17_Chr3g0144021 | 42.408 | 191 | 82 | 5 | 13 | 178 | 7 | 194 | 1.44e-38 | 131 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1076260 | AT4G35550.1 | 53.289 | 152 | 49 | 3 | 21 | 150 | 33 | 184 | 7.07e-40 | 136 |
| Msa1076260 | AT1G20700.3 | 45.333 | 150 | 57 | 5 | 19 | 143 | 24 | 173 | 1.45e-30 | 110 |
| Msa1076260 | AT1G20700.1 | 45.333 | 150 | 57 | 5 | 19 | 143 | 24 | 173 | 1.91e-30 | 110 |
| Msa1076260 | AT1G20710.1 | 44.882 | 127 | 48 | 3 | 25 | 130 | 14 | 139 | 1.34e-20 | 85.1 |
| Msa1076260 | AT1G20700.2 | 51.852 | 81 | 32 | 2 | 70 | 143 | 31 | 111 | 8.11e-18 | 76.6 |
Find 33 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAGACCAGTGTGTATAAT+TGG | 0.216618 | 7_2:+74775170 | Msa1076260:CDS |
| TACTTGCATCATTCCAATAT+TGG | 0.240745 | 7_2:-74774694 | None:intergenic |
| TTTAATCAGAACGCCAATAT+TGG | 0.268262 | 7_2:+74774681 | Msa1076260:CDS |
| ACCTGTGCAACTGCAAAGTT+TGG | 0.336692 | 7_2:+74775061 | Msa1076260:CDS |
| TGTATAATTGGTTTCAGAAT+AGG | 0.361284 | 7_2:+74775182 | Msa1076260:CDS |
| GAGATCTTGTTGAGAAGATA+AGG | 0.380614 | 7_2:-74774808 | None:intergenic |
| TCCTAGTAAGGAAAAGATAA+AGG | 0.397905 | 7_2:+74775112 | Msa1076260:intron |
| TACACACTGGTCTCTGAAAT+CGG | 0.399480 | 7_2:-74775163 | None:intergenic |
| CTCTGAAATCGGCCCGTGCT+TGG | 0.426359 | 7_2:-74775152 | None:intergenic |
| TTTGTGCAACCAGTACTTAG+AGG | 0.429969 | 7_2:+74775327 | Msa1076260:CDS |
| TCAACCTCAGTCTCTACTTC+CGG | 0.438371 | 7_2:-74775250 | None:intergenic |
| AAATAATGTTAATGATGAAC+CGG | 0.459463 | 7_2:+74775231 | Msa1076260:CDS |
| GACTGAGATGCAACGATCTC+CGG | 0.461176 | 7_2:-74775295 | None:intergenic |
| CGAATATTTGAAGCTGAGAC+GGG | 0.462716 | 7_2:+74775086 | Msa1076260:CDS |
| ATGAACAAGTCGAAACTCTT+CGG | 0.463263 | 7_2:+74774730 | Msa1076260:CDS |
| TCGTAAACTGTCATCTGATT+CGG | 0.469903 | 7_2:-74775359 | None:intergenic |
| TCCAAACTTTGCAGTTGCAC+AGG | 0.484501 | 7_2:-74775062 | None:intergenic |
| CTACTAGGCTGAGGCAGCGT+TGG | 0.489931 | 7_2:+74775030 | Msa1076260:CDS |
| CAATGATAAGAAAACTGAAC+CGG | 0.495461 | 7_2:+74775276 | Msa1076260:CDS |
| AAGAGTTTCGACTTGTTCAT+CGG | 0.503160 | 7_2:-74774727 | None:intergenic |
| AATTCAAGTCCTCTAAGTAC+TGG | 0.510452 | 7_2:-74775336 | None:intergenic |
| GGTTTCAGAATAGGCGTGCT+AGG | 0.518890 | 7_2:+74775191 | Msa1076260:CDS |
| GCGAATATTTGAAGCTGAGA+CGG | 0.525730 | 7_2:+74775085 | Msa1076260:CDS |
| CTTTGCAGTTGCACAGGTGT+TGG | 0.529341 | 7_2:-74775056 | None:intergenic |
| GAATAGGCGTGCTAGGTCAA+AGG | 0.534265 | 7_2:+74775198 | Msa1076260:CDS |
| AATAGGCGTGCTAGGTCAAA+GGG | 0.534387 | 7_2:+74775199 | Msa1076260:CDS |
| CAACAAGATCTCACTCTCAC+TGG | 0.535564 | 7_2:+74774819 | Msa1076260:CDS |
| AACGCTGATTTAACCAAGCA+CGG | 0.551145 | 7_2:+74775139 | Msa1076260:CDS |
| GAGATGGAGTGGCAGAAGAA+CGG | 0.607848 | 7_2:+74774657 | Msa1076260:CDS |
| ACGCTGATTTAACCAAGCAC+GGG | 0.639314 | 7_2:+74775140 | Msa1076260:CDS |
| CTGAAACCAATTATACACAC+TGG | 0.642488 | 7_2:-74775176 | None:intergenic |
| GAATATTTGAAGCTGAGACG+GGG | 0.658394 | 7_2:+74775087 | Msa1076260:CDS |
| TGAACCGGAAGTAGAGACTG+AGG | 0.698771 | 7_2:+74775246 | Msa1076260:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACATTTAATGATGATTTTT+AGG | + | chr7_2:74775005-74775024 | Msa1076260:intron | 15.0% |
| !!! | ACATTTAATGATGATTTTTA+GGG | + | chr7_2:74775006-74775025 | Msa1076260:intron | 15.0% |
| !!! | AACATTTAATGATGATTTTT+AGG | + | chr7_2:74775005-74775024 | Msa1076260:intron | 15.0% |
| !!! | ACATTTAATGATGATTTTTA+GGG | + | chr7_2:74775006-74775025 | Msa1076260:intron | 15.0% |
| !! | AAATAATGTTAATGATGAAC+CGG | + | chr7_2:74775231-74775250 | Msa1076260:CDS | 20.0% |
| !! | AAATAATGTTAATGATGAAC+CGG | + | chr7_2:74775231-74775250 | Msa1076260:CDS | 20.0% |
| !! | TGTATAATTGGTTTCAGAAT+AGG | + | chr7_2:74775182-74775201 | Msa1076260:CDS | 25.0% |
| !! | TGTATAATTGGTTTCAGAAT+AGG | + | chr7_2:74775182-74775201 | Msa1076260:CDS | 25.0% |
| TTTAATCAGAACGCCAATAT+TGG | + | chr7_2:74774681-74774700 | Msa1076260:CDS | 30.0% | |
| TACTTGCATCATTCCAATAT+TGG | - | chr7_2:74774697-74774716 | None:intergenic | 30.0% | |
| TCCTAGTAAGGAAAAGATAA+AGG | + | chr7_2:74775112-74775131 | Msa1076260:intron | 30.0% | |
| ! | TCCTTTATCTTTTCCTTACT+AGG | - | chr7_2:74775116-74775135 | None:intergenic | 30.0% |
| CAATGATAAGAAAACTGAAC+CGG | + | chr7_2:74775276-74775295 | Msa1076260:CDS | 30.0% | |
| TTTAATCAGAACGCCAATAT+TGG | + | chr7_2:74774681-74774700 | Msa1076260:CDS | 30.0% | |
| TACTTGCATCATTCCAATAT+TGG | - | chr7_2:74774697-74774716 | None:intergenic | 30.0% | |
| TCCTAGTAAGGAAAAGATAA+AGG | + | chr7_2:74775112-74775131 | Msa1076260:intron | 30.0% | |
| ! | TCCTTTATCTTTTCCTTACT+AGG | - | chr7_2:74775116-74775135 | None:intergenic | 30.0% |
| CAATGATAAGAAAACTGAAC+CGG | + | chr7_2:74775276-74775295 | Msa1076260:CDS | 30.0% | |
| ! | AAGAGTTTCGACTTGTTCAT+CGG | - | chr7_2:74774730-74774749 | None:intergenic | 35.0% |
| ATGAACAAGTCGAAACTCTT+CGG | + | chr7_2:74774730-74774749 | Msa1076260:CDS | 35.0% | |
| GAGATCTTGTTGAGAAGATA+AGG | - | chr7_2:74774811-74774830 | None:intergenic | 35.0% | |
| CTTCAGTTACTCATTCTCAA+CGG | + | chr7_2:74774863-74774882 | Msa1076260:intron | 35.0% | |
| ! | ACCGGTTCATTATATTGAAC+CGG | - | chr7_2:74774912-74774931 | None:intergenic | 35.0% |
| TAATGAACCGGTCTTTAGTA+CGG | + | chr7_2:74774920-74774939 | Msa1076260:intron | 35.0% | |
| !!! | GATGATTTTTAGGGTCTACT+AGG | + | chr7_2:74775015-74775034 | Msa1076260:intron | 35.0% |
| CTGAAACCAATTATACACAC+TGG | - | chr7_2:74775179-74775198 | None:intergenic | 35.0% | |
| ! | CGGTTCAGTTTTCTTATCAT+TGG | - | chr7_2:74775278-74775297 | None:intergenic | 35.0% |
| AATTCAAGTCCTCTAAGTAC+TGG | - | chr7_2:74775339-74775358 | None:intergenic | 35.0% | |
| TCGTAAACTGTCATCTGATT+CGG | - | chr7_2:74775362-74775381 | None:intergenic | 35.0% | |
| ! | AAGAGTTTCGACTTGTTCAT+CGG | - | chr7_2:74774730-74774749 | None:intergenic | 35.0% |
| ATGAACAAGTCGAAACTCTT+CGG | + | chr7_2:74774730-74774749 | Msa1076260:CDS | 35.0% | |
| GAGATCTTGTTGAGAAGATA+AGG | - | chr7_2:74774811-74774830 | None:intergenic | 35.0% | |
| CTTCAGTTACTCATTCTCAA+CGG | + | chr7_2:74774863-74774882 | Msa1076260:intron | 35.0% | |
| ! | ACCGGTTCATTATATTGAAC+CGG | - | chr7_2:74774912-74774931 | None:intergenic | 35.0% |
| TAATGAACCGGTCTTTAGTA+CGG | + | chr7_2:74774920-74774939 | Msa1076260:intron | 35.0% | |
| !!! | GATGATTTTTAGGGTCTACT+AGG | + | chr7_2:74775015-74775034 | Msa1076260:intron | 35.0% |
| CTGAAACCAATTATACACAC+TGG | - | chr7_2:74775179-74775198 | None:intergenic | 35.0% | |
| ! | CGGTTCAGTTTTCTTATCAT+TGG | - | chr7_2:74775278-74775297 | None:intergenic | 35.0% |
| AATTCAAGTCCTCTAAGTAC+TGG | - | chr7_2:74775339-74775358 | None:intergenic | 35.0% | |
| TCGTAAACTGTCATCTGATT+CGG | - | chr7_2:74775362-74775381 | None:intergenic | 35.0% | |
| TCAACGGTATCACAGTTAAC+CGG | + | chr7_2:74774879-74774898 | Msa1076260:intron | 40.0% | |
| CCGGTTCATTATATTGAACC+GGG | - | chr7_2:74774911-74774930 | None:intergenic | 40.0% | |
| CCCGGTTCAATATAATGAAC+CGG | + | chr7_2:74774908-74774927 | Msa1076260:intron | 40.0% | |
| AACCAAACCGTACTAAAGAC+CGG | - | chr7_2:74774930-74774949 | None:intergenic | 40.0% | |
| ! | GCGAATATTTGAAGCTGAGA+CGG | + | chr7_2:74775085-74775104 | Msa1076260:CDS | 40.0% |
| ! | CGAATATTTGAAGCTGAGAC+GGG | + | chr7_2:74775086-74775105 | Msa1076260:CDS | 40.0% |
| ! | GAATATTTGAAGCTGAGACG+GGG | + | chr7_2:74775087-74775106 | Msa1076260:CDS | 40.0% |
| !! | AACGCTGATTTAACCAAGCA+CGG | + | chr7_2:74775139-74775158 | Msa1076260:CDS | 40.0% |
| !! | TACACACTGGTCTCTGAAAT+CGG | - | chr7_2:74775166-74775185 | None:intergenic | 40.0% |
| CAGAGACCAGTGTGTATAAT+TGG | + | chr7_2:74775170-74775189 | Msa1076260:CDS | 40.0% | |
| TTTGTGCAACCAGTACTTAG+AGG | + | chr7_2:74775327-74775346 | Msa1076260:CDS | 40.0% | |
| TCAACGGTATCACAGTTAAC+CGG | + | chr7_2:74774879-74774898 | Msa1076260:intron | 40.0% | |
| CCGGTTCATTATATTGAACC+GGG | - | chr7_2:74774911-74774930 | None:intergenic | 40.0% | |
| CCCGGTTCAATATAATGAAC+CGG | + | chr7_2:74774908-74774927 | Msa1076260:intron | 40.0% | |
| AACCAAACCGTACTAAAGAC+CGG | - | chr7_2:74774930-74774949 | None:intergenic | 40.0% | |
| ! | GCGAATATTTGAAGCTGAGA+CGG | + | chr7_2:74775085-74775104 | Msa1076260:CDS | 40.0% |
| ! | CGAATATTTGAAGCTGAGAC+GGG | + | chr7_2:74775086-74775105 | Msa1076260:CDS | 40.0% |
| ! | GAATATTTGAAGCTGAGACG+GGG | + | chr7_2:74775087-74775106 | Msa1076260:CDS | 40.0% |
| !! | AACGCTGATTTAACCAAGCA+CGG | + | chr7_2:74775139-74775158 | Msa1076260:CDS | 40.0% |
| !! | TACACACTGGTCTCTGAAAT+CGG | - | chr7_2:74775166-74775185 | None:intergenic | 40.0% |
| CAGAGACCAGTGTGTATAAT+TGG | + | chr7_2:74775170-74775189 | Msa1076260:CDS | 40.0% | |
| TTTGTGCAACCAGTACTTAG+AGG | + | chr7_2:74775327-74775346 | Msa1076260:CDS | 40.0% | |
| CAACAAGATCTCACTCTCAC+TGG | + | chr7_2:74774819-74774838 | Msa1076260:CDS | 45.0% | |
| CAACGGTATCACAGTTAACC+GGG | + | chr7_2:74774880-74774899 | Msa1076260:intron | 45.0% | |
| AACCGGTCTTTAGTACGGTT+TGG | + | chr7_2:74774925-74774944 | Msa1076260:intron | 45.0% | |
| !!! | TTTTAGGGTCTACTAGGCTG+AGG | + | chr7_2:74775021-74775040 | Msa1076260:intron | 45.0% |
| ACCTGTGCAACTGCAAAGTT+TGG | + | chr7_2:74775061-74775080 | Msa1076260:CDS | 45.0% | |
| TCCAAACTTTGCAGTTGCAC+AGG | - | chr7_2:74775065-74775084 | None:intergenic | 45.0% | |
| !! | ACGCTGATTTAACCAAGCAC+GGG | + | chr7_2:74775140-74775159 | Msa1076260:CDS | 45.0% |
| AATAGGCGTGCTAGGTCAAA+GGG | + | chr7_2:74775199-74775218 | Msa1076260:CDS | 45.0% | |
| TCAACCTCAGTCTCTACTTC+CGG | - | chr7_2:74775253-74775272 | None:intergenic | 45.0% | |
| CAACAAGATCTCACTCTCAC+TGG | + | chr7_2:74774819-74774838 | Msa1076260:CDS | 45.0% | |
| CAACGGTATCACAGTTAACC+GGG | + | chr7_2:74774880-74774899 | Msa1076260:intron | 45.0% | |
| AACCGGTCTTTAGTACGGTT+TGG | + | chr7_2:74774925-74774944 | Msa1076260:intron | 45.0% | |
| !!! | TTTTAGGGTCTACTAGGCTG+AGG | + | chr7_2:74775021-74775040 | Msa1076260:intron | 45.0% |
| ACCTGTGCAACTGCAAAGTT+TGG | + | chr7_2:74775061-74775080 | Msa1076260:CDS | 45.0% | |
| TCCAAACTTTGCAGTTGCAC+AGG | - | chr7_2:74775065-74775084 | None:intergenic | 45.0% | |
| !! | ACGCTGATTTAACCAAGCAC+GGG | + | chr7_2:74775140-74775159 | Msa1076260:CDS | 45.0% |
| AATAGGCGTGCTAGGTCAAA+GGG | + | chr7_2:74775199-74775218 | Msa1076260:CDS | 45.0% | |
| TCAACCTCAGTCTCTACTTC+CGG | - | chr7_2:74775253-74775272 | None:intergenic | 45.0% | |
| GAGATGGAGTGGCAGAAGAA+CGG | + | chr7_2:74774657-74774676 | Msa1076260:CDS | 50.0% | |
| CTTTGCAGTTGCACAGGTGT+TGG | - | chr7_2:74775059-74775078 | None:intergenic | 50.0% | |
| GGTTTCAGAATAGGCGTGCT+AGG | + | chr7_2:74775191-74775210 | Msa1076260:CDS | 50.0% | |
| GAATAGGCGTGCTAGGTCAA+AGG | + | chr7_2:74775198-74775217 | Msa1076260:CDS | 50.0% | |
| TGAACCGGAAGTAGAGACTG+AGG | + | chr7_2:74775246-74775265 | Msa1076260:CDS | 50.0% | |
| GACTGAGATGCAACGATCTC+CGG | - | chr7_2:74775298-74775317 | None:intergenic | 50.0% | |
| GAGATGGAGTGGCAGAAGAA+CGG | + | chr7_2:74774657-74774676 | Msa1076260:CDS | 50.0% | |
| CTTTGCAGTTGCACAGGTGT+TGG | - | chr7_2:74775059-74775078 | None:intergenic | 50.0% | |
| GGTTTCAGAATAGGCGTGCT+AGG | + | chr7_2:74775191-74775210 | Msa1076260:CDS | 50.0% | |
| GAATAGGCGTGCTAGGTCAA+AGG | + | chr7_2:74775198-74775217 | Msa1076260:CDS | 50.0% | |
| TGAACCGGAAGTAGAGACTG+AGG | + | chr7_2:74775246-74775265 | Msa1076260:CDS | 50.0% | |
| GACTGAGATGCAACGATCTC+CGG | - | chr7_2:74775298-74775317 | None:intergenic | 50.0% | |
| CGGTATCACAGTTAACCGGG+TGG | + | chr7_2:74774883-74774902 | Msa1076260:intron | 55.0% | |
| ATATTGAACCGGGCTCCACC+CGG | - | chr7_2:74774901-74774920 | None:intergenic | 55.0% | |
| TGAGACGGGGACTCCTAGTA+AGG | + | chr7_2:74775100-74775119 | Msa1076260:intron | 55.0% | |
| CGGTATCACAGTTAACCGGG+TGG | + | chr7_2:74774883-74774902 | Msa1076260:intron | 55.0% | |
| ATATTGAACCGGGCTCCACC+CGG | - | chr7_2:74774901-74774920 | None:intergenic | 55.0% | |
| TGAGACGGGGACTCCTAGTA+AGG | + | chr7_2:74775100-74775119 | Msa1076260:intron | 55.0% | |
| ACAGTTAACCGGGTGGAGCC+CGG | + | chr7_2:74774890-74774909 | Msa1076260:intron | 60.0% | |
| CTACTAGGCTGAGGCAGCGT+TGG | + | chr7_2:74775030-74775049 | Msa1076260:CDS | 60.0% | |
| !! | CTCTGAAATCGGCCCGTGCT+TGG | - | chr7_2:74775155-74775174 | None:intergenic | 60.0% |
| ACAGTTAACCGGGTGGAGCC+CGG | + | chr7_2:74774890-74774909 | Msa1076260:intron | 60.0% | |
| CTACTAGGCTGAGGCAGCGT+TGG | + | chr7_2:74775030-74775049 | Msa1076260:CDS | 60.0% | |
| !! | CTCTGAAATCGGCCCGTGCT+TGG | - | chr7_2:74775155-74775174 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 74774648 | 74775396 | 74774648 | ID=Msa1076260;Name=Msa1076260 |
| chr7_2 | mRNA | 74774648 | 74775396 | 74774648 | ID=Msa1076260-mRNA-1;Parent=Msa1076260;Name=Msa1076260-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|0|0|3|0|185 |
| chr7_2 | exon | 74774648 | 74774840 | 74774648 | ID=Msa1076260-mRNA-1:exon:17668;Parent=Msa1076260-mRNA-1 |
| chr7_2 | exon | 74775027 | 74775116 | 74775027 | ID=Msa1076260-mRNA-1:exon:17669;Parent=Msa1076260-mRNA-1 |
| chr7_2 | exon | 74775122 | 74775396 | 74775122 | ID=Msa1076260-mRNA-1:exon:17670;Parent=Msa1076260-mRNA-1 |
| chr7_2 | CDS | 74774648 | 74774840 | 74774648 | ID=Msa1076260-mRNA-1:cds;Parent=Msa1076260-mRNA-1 |
| chr7_2 | CDS | 74775027 | 74775116 | 74775027 | ID=Msa1076260-mRNA-1:cds;Parent=Msa1076260-mRNA-1 |
| chr7_2 | CDS | 74775122 | 74775396 | 74775122 | ID=Msa1076260-mRNA-1:cds;Parent=Msa1076260-mRNA-1 |
| Gene Sequence |
| Protein sequence |
