Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1076300 | KEH23820.1 | 80.851 | 47 | 8 | 1 | 32 | 77 | 10 | 56 | 2.82e-19 | 85.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1076300 | sp|Q8W3L9|EIL2_ORYSJ | 68.293 | 41 | 13 | 0 | 37 | 77 | 148 | 188 | 8.74e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1076300 | A0A072UDC0 | 80.851 | 47 | 8 | 1 | 32 | 77 | 10 | 56 | 1.35e-19 | 85.5 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1076300 | TF | EIL |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1076300 | MtrunA17_Chr7g0260281 | 80.851 | 47 | 8 | 1 | 32 | 77 | 10 | 56 | 7.49e-23 | 85.1 |
| Msa1076300 | MtrunA17_Chr2g0329151 | 72.727 | 33 | 9 | 0 | 45 | 77 | 106 | 138 | 1.64e-12 | 61.2 |
| Msa1076300 | MtrunA17_Chr5g0440591 | 75.758 | 33 | 8 | 0 | 45 | 77 | 115 | 147 | 2.86e-12 | 60.5 |
| Msa1076300 | MtrunA17_Chr3g0118361 | 75.758 | 33 | 8 | 0 | 45 | 77 | 118 | 150 | 3.59e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1076300 | AT3G20770.1 | 78.788 | 33 | 7 | 0 | 45 | 77 | 115 | 147 | 9.62e-13 | 62.4 |
| Msa1076300 | AT1G73730.1 | 69.697 | 33 | 10 | 0 | 45 | 77 | 106 | 138 | 9.09e-12 | 59.7 |
| Msa1076300 | AT1G73730.2 | 69.697 | 33 | 10 | 0 | 45 | 77 | 94 | 126 | 9.72e-12 | 59.3 |
| Msa1076300 | AT2G27050.1 | 75.758 | 33 | 8 | 0 | 45 | 77 | 116 | 148 | 1.05e-11 | 59.3 |
Find 14 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGCAATGAAGAAAGAAATT+TGG | 0.267238 | 7_2:+74851210 | Msa1076300:CDS |
| TACGGAATTATACCTGAAAA+TGG | 0.269291 | 7_2:+74851395 | Msa1076300:CDS |
| GAATATAAACGGTGGTATCA+TGG | 0.436629 | 7_2:+74851235 | Msa1076300:CDS |
| CTGAAAATCTTCACTGTTGG+TGG | 0.437559 | 7_2:+74851438 | Msa1076300:CDS |
| GAAATTTGGAGGAATATAAA+CGG | 0.490162 | 7_2:+74851224 | Msa1076300:CDS |
| ATTGTAGAACAAGCACAAGT+TGG | 0.508697 | 7_2:+74851346 | Msa1076300:intron |
| ATGATCATGTTGTATCAGAA+AGG | 0.538951 | 7_2:+74851486 | Msa1076300:CDS |
| CAATGAAGAAAGAAATTTGG+AGG | 0.552046 | 7_2:+74851213 | Msa1076300:CDS |
| CATCTGAAAATCTTCACTGT+TGG | 0.553597 | 7_2:+74851435 | Msa1076300:CDS |
| AACAAGCACAAGTTGGAAGA+TGG | 0.555633 | 7_2:+74851353 | Msa1076300:CDS |
| AAATCTTCACTGTTGGTGGA+AGG | 0.565627 | 7_2:+74851442 | Msa1076300:CDS |
| CATTCTGATGAATTTGTGTA+CGG | 0.572592 | 7_2:+74851377 | Msa1076300:CDS |
| ATTTGGAGGAATATAAACGG+TGG | 0.680669 | 7_2:+74851227 | Msa1076300:CDS |
| AGTACACAAGTTGAAGACAA+AGG | 0.686224 | 7_2:+74851190 | Msa1076300:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTGAACAAGATAAAAA+AGG | + | chr7_2:74851296-74851315 | Msa1076300:intron | 15.0% |
| ! | GAAATTTGGAGGAATATAAA+CGG | + | chr7_2:74851224-74851243 | Msa1076300:CDS | 25.0% |
| ! | GGAATTGAAAAATCATAGTA+AGG | + | chr7_2:74851256-74851275 | Msa1076300:CDS | 25.0% |
| !! | TTATCTTGTTCAATTTTTGC+TGG | - | chr7_2:74851293-74851312 | None:intergenic | 25.0% |
| AGGCAATGAAGAAAGAAATT+TGG | + | chr7_2:74851210-74851229 | Msa1076300:CDS | 30.0% | |
| CAATGAAGAAAGAAATTTGG+AGG | + | chr7_2:74851213-74851232 | Msa1076300:CDS | 30.0% | |
| CATTCTGATGAATTTGTGTA+CGG | + | chr7_2:74851377-74851396 | Msa1076300:CDS | 30.0% | |
| TACGGAATTATACCTGAAAA+TGG | + | chr7_2:74851395-74851414 | Msa1076300:CDS | 30.0% | |
| !!! | CTTATTGGTTTTCCATTTTC+AGG | - | chr7_2:74851410-74851429 | None:intergenic | 30.0% |
| ! | TTTTCAGATGCAACACTTAT+TGG | - | chr7_2:74851425-74851444 | None:intergenic | 30.0% |
| ATGATCATGTTGTATCAGAA+AGG | + | chr7_2:74851486-74851505 | Msa1076300:CDS | 30.0% | |
| ! | AGTACACAAGTTGAAGACAA+AGG | + | chr7_2:74851190-74851209 | Msa1076300:CDS | 35.0% |
| ATTTGGAGGAATATAAACGG+TGG | + | chr7_2:74851227-74851246 | Msa1076300:CDS | 35.0% | |
| GAATATAAACGGTGGTATCA+TGG | + | chr7_2:74851235-74851254 | Msa1076300:CDS | 35.0% | |
| ATTGTAGAACAAGCACAAGT+TGG | + | chr7_2:74851346-74851365 | Msa1076300:intron | 35.0% | |
| CATCTGAAAATCTTCACTGT+TGG | + | chr7_2:74851435-74851454 | Msa1076300:CDS | 35.0% | |
| ! | AACAAGCACAAGTTGGAAGA+TGG | + | chr7_2:74851353-74851372 | Msa1076300:CDS | 40.0% |
| CTGAAAATCTTCACTGTTGG+TGG | + | chr7_2:74851438-74851457 | Msa1076300:CDS | 40.0% | |
| ! | AAATCTTCACTGTTGGTGGA+AGG | + | chr7_2:74851442-74851461 | Msa1076300:CDS | 40.0% |
| ! | GTTGGTGGAAGGAAAAAGTG+AGG | + | chr7_2:74851453-74851472 | Msa1076300:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 74851185 | 74851520 | 74851185 | ID=Msa1076300;Name=Msa1076300 |
| chr7_2 | mRNA | 74851185 | 74851520 | 74851185 | ID=Msa1076300-mRNA-1;Parent=Msa1076300;Name=Msa1076300-mRNA-1;_AED=0.31;_eAED=0.44;_QI=0|0|0|0.5|0|0.5|2|0|87 |
| chr7_2 | exon | 74851185 | 74851280 | 74851185 | ID=Msa1076300-mRNA-1:exon:17689;Parent=Msa1076300-mRNA-1 |
| chr7_2 | exon | 74851353 | 74851520 | 74851353 | ID=Msa1076300-mRNA-1:exon:17690;Parent=Msa1076300-mRNA-1 |
| chr7_2 | CDS | 74851185 | 74851280 | 74851185 | ID=Msa1076300-mRNA-1:cds;Parent=Msa1076300-mRNA-1 |
| chr7_2 | CDS | 74851353 | 74851520 | 74851353 | ID=Msa1076300-mRNA-1:cds;Parent=Msa1076300-mRNA-1 |
| Gene Sequence |
| Protein sequence |
