Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1081920 | KEH19406.1 | 66.087 | 115 | 37 | 1 | 7 | 121 | 28 | 140 | 5.11e-42 | 146 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1081920 | sp|Q9FNG6|C3H51_ARATH | 42.593 | 162 | 43 | 2 | 17 | 128 | 129 | 290 | 1.99e-31 | 118 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1081920 | A0A072TQE4 | 66.087 | 115 | 37 | 1 | 7 | 121 | 28 | 140 | 2.44e-42 | 146 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1081920 | TF | C3H |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1081920 | MtrunA17_Chr7g0269781 | 47.531 | 162 | 32 | 2 | 13 | 121 | 80 | 241 | 3.75e-37 | 129 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1081920 | AT5G06420.1 | 42.593 | 162 | 43 | 2 | 17 | 128 | 129 | 290 | 2.03e-32 | 118 |
| Msa1081920 | AT5G06420.2 | 42.593 | 162 | 43 | 2 | 17 | 128 | 129 | 290 | 2.03e-32 | 118 |
| Msa1081920 | AT1G01350.1 | 41.975 | 162 | 44 | 2 | 17 | 128 | 94 | 255 | 3.77e-31 | 114 |
Find 26 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGCACACTCTTTGCTTTCT+CGG | 0.302154 | 7_2:+81818404 | None:intergenic |
| TGAAGACTCAAAATGAAAGA+TGG | 0.383661 | 7_2:+81818694 | None:intergenic |
| TTGAAGTCTTTGCATATGTC+AGG | 0.387439 | 7_2:+81818524 | None:intergenic |
| TGACATATGCAAAGACTTCA+AGG | 0.411330 | 7_2:-81818522 | Msa1081920:CDS |
| AAGCAAAGAGTGTGCGCTAA+TGG | 0.422128 | 7_2:-81818398 | Msa1081920:CDS |
| AAAATGAAAGATGGGCTTCT+CGG | 0.426527 | 7_2:+81818703 | None:intergenic |
| TGCAAAGACTTCAAGGAGAC+TGG | 0.432731 | 7_2:-81818515 | Msa1081920:CDS |
| TAGCAAAGCAACAGCAACTT+TGG | 0.436558 | 7_2:-81818648 | Msa1081920:CDS |
| GAAGACTCAAAATGAAAGAT+GGG | 0.465277 | 7_2:+81818695 | None:intergenic |
| GACCGAGGCAACTACAAGTC+CGG | 0.465608 | 7_2:-81818456 | Msa1081920:CDS |
| GGTGGCAGTTGGACAAAGAA+TGG | 0.496757 | 7_2:-81818434 | Msa1081920:CDS |
| TTGTCTGTTTGTTTGTCTGA+AGG | 0.512605 | 7_2:-81818374 | Msa1081920:CDS |
| ACCCGGACTTGTAGTTGCCT+CGG | 0.520056 | 7_2:+81818454 | None:intergenic |
| ACCGAGGCAACTACAAGTCC+GGG | 0.533404 | 7_2:-81818455 | Msa1081920:CDS |
| GAGACTGGGTATTGTGGCTA+CGG | 0.541621 | 7_2:-81818500 | Msa1081920:CDS |
| TTCTTTGTCCAACTGCCACC+CGG | 0.548782 | 7_2:+81818437 | None:intergenic |
| TTCAAGGAGACTGGGTATTG+TGG | 0.553969 | 7_2:-81818506 | Msa1081920:CDS |
| CTACAAGTCCGGGTGGCAGT+TGG | 0.571560 | 7_2:-81818445 | Msa1081920:CDS |
| GAAACTCTAATATGAGTCGA+AGG | 0.595574 | 7_2:+81818566 | None:intergenic |
| ATCCGTGGGTCACACTCACA+CGG | 0.602004 | 7_2:-81818599 | Msa1081920:CDS |
| GCAAAGACTTCAAGGAGACT+GGG | 0.620309 | 7_2:-81818514 | Msa1081920:CDS |
| GTGTGAGTGTGACCCACGGA+TGG | 0.640412 | 7_2:+81818601 | None:intergenic |
| ATGCAAGTTCTTCACGACCG+AGG | 0.651233 | 7_2:-81818471 | Msa1081920:CDS |
| GACCGTGTGAGTGTGACCCA+CGG | 0.684896 | 7_2:+81818597 | None:intergenic |
| ATATGAGTCGAAGGTCTGAG+AGG | 0.697705 | 7_2:+81818575 | None:intergenic |
| GAGGCAACTACAAGTCCGGG+TGG | 0.723997 | 7_2:-81818452 | Msa1081920:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTGTCTGAAGGAAAAAAAA+GGG | - | chr7_2:81818711-81818730 | Msa1081920:CDS | 25.0% |
| GAGAAAAAGAACATTCAAGA+AGG | - | chr7_2:81818330-81818349 | Msa1081920:CDS | 30.0% | |
| GAAGACTCAAAATGAAAGAT+GGG | + | chr7_2:81818382-81818401 | None:intergenic | 30.0% | |
| TGAAGACTCAAAATGAAAGA+TGG | + | chr7_2:81818383-81818402 | None:intergenic | 30.0% | |
| GTTTGTCTGAAGGAAAAAAA+AGG | - | chr7_2:81818710-81818729 | Msa1081920:CDS | 30.0% | |
| TGAAGGAAAAAAAAGGGAAA+AGG | - | chr7_2:81818717-81818736 | Msa1081920:CDS | 30.0% | |
| ! | AAAATGAAAGATGGGCTTCT+CGG | + | chr7_2:81818374-81818393 | None:intergenic | 35.0% |
| !!! | ATTTTTCAAGTTCCATCCGT+GGG | - | chr7_2:81818461-81818480 | Msa1081920:CDS | 35.0% |
| GAAACTCTAATATGAGTCGA+AGG | + | chr7_2:81818511-81818530 | None:intergenic | 35.0% | |
| TTGAAGTCTTTGCATATGTC+AGG | + | chr7_2:81818553-81818572 | None:intergenic | 35.0% | |
| TGACATATGCAAAGACTTCA+AGG | - | chr7_2:81818552-81818571 | Msa1081920:CDS | 35.0% | |
| TTGTCTGTTTGTTTGTCTGA+AGG | - | chr7_2:81818700-81818719 | Msa1081920:CDS | 35.0% | |
| AAAAAGGGAAAAGGACTTTC+TGG | - | chr7_2:81818726-81818745 | Msa1081920:CDS | 35.0% | |
| TAGCAAAGCAACAGCAACTT+TGG | - | chr7_2:81818426-81818445 | Msa1081920:CDS | 40.0% | |
| !!! | CATTTTTCAAGTTCCATCCG+TGG | - | chr7_2:81818460-81818479 | Msa1081920:CDS | 40.0% |
| ATATGAGTCGAAGGTCTGAG+AGG | + | chr7_2:81818502-81818521 | None:intergenic | 45.0% | |
| TGCAAAGACTTCAAGGAGAC+TGG | - | chr7_2:81818559-81818578 | Msa1081920:CDS | 45.0% | |
| GCAAAGACTTCAAGGAGACT+GGG | - | chr7_2:81818560-81818579 | Msa1081920:CDS | 45.0% | |
| ! | TTCAAGGAGACTGGGTATTG+TGG | - | chr7_2:81818568-81818587 | Msa1081920:CDS | 45.0% |
| ! | AAGCAAAGAGTGTGCGCTAA+TGG | - | chr7_2:81818676-81818695 | Msa1081920:CDS | 45.0% |
| ! | GAGACTGGGTATTGTGGCTA+CGG | - | chr7_2:81818574-81818593 | Msa1081920:CDS | 50.0% |
| ATGCAAGTTCTTCACGACCG+AGG | - | chr7_2:81818603-81818622 | Msa1081920:CDS | 50.0% | |
| TTCTTTGTCCAACTGCCACC+CGG | + | chr7_2:81818640-81818659 | None:intergenic | 50.0% | |
| GGTGGCAGTTGGACAAAGAA+TGG | - | chr7_2:81818640-81818659 | Msa1081920:CDS | 50.0% | |
| GCGCACACTCTTTGCTTTCT+CGG | + | chr7_2:81818673-81818692 | None:intergenic | 50.0% | |
| ATCCGTGGGTCACACTCACA+CGG | - | chr7_2:81818475-81818494 | Msa1081920:CDS | 55.0% | |
| GACCGAGGCAACTACAAGTC+CGG | - | chr7_2:81818618-81818637 | Msa1081920:CDS | 55.0% | |
| ACCGAGGCAACTACAAGTCC+GGG | - | chr7_2:81818619-81818638 | Msa1081920:CDS | 55.0% | |
| ACCCGGACTTGTAGTTGCCT+CGG | + | chr7_2:81818623-81818642 | None:intergenic | 55.0% | |
| GTGTGAGTGTGACCCACGGA+TGG | + | chr7_2:81818476-81818495 | None:intergenic | 60.0% | |
| GACCGTGTGAGTGTGACCCA+CGG | + | chr7_2:81818480-81818499 | None:intergenic | 60.0% | |
| GAGGCAACTACAAGTCCGGG+TGG | - | chr7_2:81818622-81818641 | Msa1081920:CDS | 60.0% | |
| CTACAAGTCCGGGTGGCAGT+TGG | - | chr7_2:81818629-81818648 | Msa1081920:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 81818325 | 81818771 | 81818325 | ID=Msa1081920;Name=Msa1081920 |
| chr7_2 | mRNA | 81818325 | 81818771 | 81818325 | ID=Msa1081920-mRNA-1;Parent=Msa1081920;Name=Msa1081920-mRNA-1;_AED=0.48;_eAED=0.51;_QI=0|-1|0|1|-1|1|1|0|148 |
| chr7_2 | exon | 81818325 | 81818771 | 81818325 | ID=Msa1081920-mRNA-1:exon:21097;Parent=Msa1081920-mRNA-1 |
| chr7_2 | CDS | 81818325 | 81818771 | 81818325 | ID=Msa1081920-mRNA-1:cds;Parent=Msa1081920-mRNA-1 |
| Gene Sequence |
| Protein sequence |
