AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1087450


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1087450 MtrunA17_Chr8g0337271 82.653 196 34 0 1 196 1 196 1.32e-120 339
Msa1087450 MtrunA17_Chr8g0358731 34.328 201 95 6 1 199 1 166 3.56e-26 99.0
Msa1087450 MtrunA17_Chr6g0452691 27.211 147 100 2 10 149 9 155 3.90e-12 62.4
Msa1087450 MtrunA17_Chr5g0439111 27.660 141 96 4 10 148 9 145 1.21e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1087450 AT3G50510.1 28.696 115 81 1 1 114 3 117 3.75e-14 68.2
Msa1087450 AT3G50510.2 28.696 115 81 1 1 114 3 117 3.75e-14 68.2
Msa1087450 AT2G19820.1 31.183 93 63 1 5 96 7 99 7.96e-13 63.2

Find 54 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCAAGGATGTGCTTATT+TGG 0.063042 7_3:-198400 None:intergenic
TCAGTGTGTTATTCAAATTT+TGG 0.145999 7_3:+198235 Msa1087450:CDS
CTGACTTAAACTTGAGTATA+TGG 0.197296 7_3:+198516 Msa1087450:CDS
CAAATTCCCCATCTTACTTT+GGG 0.270367 7_3:-199006 None:intergenic
CATGACACTGATTGTGAATT+TGG 0.306396 7_3:+198179 Msa1087450:CDS
ATCCAAGGATGTGCTTATTT+GGG 0.326625 7_3:-198399 None:intergenic
TCAAATTCCCCATCTTACTT+TGG 0.356414 7_3:-199007 None:intergenic
TCTTGGACACCAATTGGAGC+TGG 0.391570 7_3:+198373 Msa1087450:CDS
AAAGTCTTCATCCTCATTAC+TGG 0.397078 7_3:-198981 None:intergenic
TTCATCAGCCACCATTCCCT+TGG 0.409411 7_3:-198924 None:intergenic
TGTCCAAGAAAACCCCGATC+TGG 0.424943 7_3:-198359 None:intergenic
TTTGGGCACCCAGCTCCAAT+TGG 0.428496 7_3:-198382 None:intergenic
TTAATTGCATAATTCTCATC+AGG 0.430232 7_3:-198268 None:intergenic
GGGAATGGTGGCTGATGAAA+AGG 0.430935 7_3:+198928 Msa1087450:CDS
TGACTTAAACTTGAGTATAT+GGG 0.443514 7_3:+198517 Msa1087450:CDS
CTTGGACACCAATTGGAGCT+GGG 0.456606 7_3:+198374 Msa1087450:CDS
TTGGCGAAGTAACCCAGATC+GGG 0.470404 7_3:+198346 Msa1087450:CDS
TGCCCAAATAAGCACATCCT+TGG 0.475102 7_3:+198397 Msa1087450:CDS
AGCAATTTCAATTTCATCCA+AGG 0.478006 7_3:-198414 None:intergenic
CATATACTCAAGTTTAAGTC+AGG 0.479889 7_3:-198515 None:intergenic
TCTCCTCTTTCACAAGTACA+TGG 0.497829 7_3:-198766 None:intergenic
TCCTTCTGTGAGAAGCGAAT+CGG 0.511515 7_3:-198318 None:intergenic
TACTGAACACATGGGTGACA+TGG 0.514848 7_3:+198714 Msa1087450:CDS
GCCGATTCGCTTCTCACAGA+AGG 0.517177 7_3:+198317 Msa1087450:CDS
ATTCCATGTACTTGTGAAAG+AGG 0.523001 7_3:+198763 Msa1087450:CDS
TGTACACAGTAATGCATCCA+AGG 0.526625 7_3:+198907 Msa1087450:intron
GTCCAAGAAAACCCCGATCT+GGG 0.526848 7_3:-198358 None:intergenic
CTGAAAGTCAGCATGCCTAC+TGG 0.528158 7_3:-198216 None:intergenic
TGTGCCGACATATGAGAAGA+AGG 0.531308 7_3:+198156 Msa1087450:CDS
GTGGCTGATGAAAAGGGAAA+AGG 0.537842 7_3:+198935 Msa1087450:CDS
CTTGGCGAAGTAACCCAGAT+CGG 0.540526 7_3:+198345 Msa1087450:CDS
CATGCCTTCTTCTCATATGT+CGG 0.541385 7_3:-198160 None:intergenic
GTCTTCATCCTCATTACTGG+TGG 0.547436 7_3:-198978 None:intergenic
GCAACAATTTGTCTTTGATG+TGG 0.547669 7_3:-198296 None:intergenic
TTCTCACAGAAGGAGAAGCT+TGG 0.549947 7_3:+198327 Msa1087450:CDS
GTGCAAGAAGGCATGGAAGA+AGG 0.556829 7_3:-198485 None:intergenic
AAATTCCCCATCTTACTTTG+GGG 0.573119 7_3:-199005 None:intergenic
CAGTAATGCATCCAAGGGAA+TGG 0.575632 7_3:+198913 Msa1087450:intron
AATTTGAGTGCAAGAAGGCA+TGG 0.581783 7_3:-198492 None:intergenic
AAATGCATCAGAGAAATACA+AGG 0.593981 7_3:+198117 None:intergenic
TAATGCATCCAAGGGAATGG+TGG 0.611462 7_3:+198916 Msa1087450:CDS
ACAAGGACCCACCAGTAATG+AGG 0.613595 7_3:+198970 Msa1087450:CDS
CTTGTGAAAGAGGAGAATCA+AGG 0.617523 7_3:+198773 Msa1087450:CDS
GGAATGGTGGCTGATGAAAA+GGG 0.618900 7_3:+198929 Msa1087450:CDS
CATGGGTGACATGGACATCA+CGG 0.621521 7_3:+198723 Msa1087450:CDS
TCAGGAATTTGAGTGCAAGA+AGG 0.621914 7_3:-198497 None:intergenic
TTAATAGAGTACTGAACACA+TGG 0.626411 7_3:+198705 Msa1087450:intron
GTACACAGTAATGCATCCAA+GGG 0.631300 7_3:+198908 Msa1087450:intron
TCTTCATCCTCATTACTGGT+GGG 0.637097 7_3:-198977 None:intergenic
AAAGGAATTCTTAATGAACA+AGG 0.657272 7_3:+198953 Msa1087450:CDS
TGGCGAAGTAACCCAGATCG+GGG 0.667644 7_3:+198347 Msa1087450:CDS
ATCACGGAACAAGACATGCA+AGG 0.684891 7_3:+198739 Msa1087450:CDS
AATGCATCAGAGAAATACAA+GGG 0.687321 7_3:+198118 None:intergenic
TAATAGAGTACTGAACACAT+GGG 0.740993 7_3:+198706 Msa1087450:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAAAAGAATGATAGATTT+TGG - chr7_3:198661-198680 None:intergenic 20.0%
!!! TTGAAAAGAATGATAGATTT+TGG - chr7_3:198661-198680 None:intergenic 20.0%
!!! TCAGTGTGTTATTCAAATTT+TGG + chr7_3:198235-198254 Msa1087450:CDS 25.0%
!!! CAGTGTGTTATTCAAATTTT+GGG + chr7_3:198236-198255 Msa1087450:CDS 25.0%
!!! AGTGTGTTATTCAAATTTTG+GGG + chr7_3:198237-198256 Msa1087450:CDS 25.0%
! TTAATTGCATAATTCTCATC+AGG - chr7_3:198271-198290 None:intergenic 25.0%
! TGACTTAAACTTGAGTATAT+GGG + chr7_3:198517-198536 Msa1087450:CDS 25.0%
! AAAGGAATTCTTAATGAACA+AGG + chr7_3:198953-198972 Msa1087450:CDS 25.0%
!!! TCAGTGTGTTATTCAAATTT+TGG + chr7_3:198235-198254 Msa1087450:CDS 25.0%
!!! CAGTGTGTTATTCAAATTTT+GGG + chr7_3:198236-198255 Msa1087450:CDS 25.0%
!!! AGTGTGTTATTCAAATTTTG+GGG + chr7_3:198237-198256 Msa1087450:CDS 25.0%
! TTAATTGCATAATTCTCATC+AGG - chr7_3:198271-198290 None:intergenic 25.0%
! TGACTTAAACTTGAGTATAT+GGG + chr7_3:198517-198536 Msa1087450:CDS 25.0%
! AAAGGAATTCTTAATGAACA+AGG + chr7_3:198953-198972 Msa1087450:CDS 25.0%
AGCAATTTCAATTTCATCCA+AGG - chr7_3:198417-198436 None:intergenic 30.0%
CATATACTCAAGTTTAAGTC+AGG - chr7_3:198518-198537 None:intergenic 30.0%
CTGACTTAAACTTGAGTATA+TGG + chr7_3:198516-198535 Msa1087450:CDS 30.0%
ATGTGTTCAGTACTCTATTA+AGG - chr7_3:198706-198725 None:intergenic 30.0%
!! TTAATAGAGTACTGAACACA+TGG + chr7_3:198705-198724 Msa1087450:intron 30.0%
!! TAATAGAGTACTGAACACAT+GGG + chr7_3:198706-198725 Msa1087450:intron 30.0%
AGCAATTTCAATTTCATCCA+AGG - chr7_3:198417-198436 None:intergenic 30.0%
CATATACTCAAGTTTAAGTC+AGG - chr7_3:198518-198537 None:intergenic 30.0%
CTGACTTAAACTTGAGTATA+TGG + chr7_3:198516-198535 Msa1087450:CDS 30.0%
ATGTGTTCAGTACTCTATTA+AGG - chr7_3:198706-198725 None:intergenic 30.0%
!! TTAATAGAGTACTGAACACA+TGG + chr7_3:198705-198724 Msa1087450:intron 30.0%
!! TAATAGAGTACTGAACACAT+GGG + chr7_3:198706-198725 Msa1087450:intron 30.0%
! CATGACACTGATTGTGAATT+TGG + chr7_3:198179-198198 Msa1087450:CDS 35.0%
GCAACAATTTGTCTTTGATG+TGG - chr7_3:198299-198318 None:intergenic 35.0%
ATCCAAGGATGTGCTTATTT+GGG - chr7_3:198402-198421 None:intergenic 35.0%
ATTCCATGTACTTGTGAAAG+AGG + chr7_3:198763-198782 Msa1087450:CDS 35.0%
AAAGTCTTCATCCTCATTAC+TGG - chr7_3:198984-199003 None:intergenic 35.0%
! CATGACACTGATTGTGAATT+TGG + chr7_3:198179-198198 Msa1087450:CDS 35.0%
GCAACAATTTGTCTTTGATG+TGG - chr7_3:198299-198318 None:intergenic 35.0%
ATCCAAGGATGTGCTTATTT+GGG - chr7_3:198402-198421 None:intergenic 35.0%
ATTCCATGTACTTGTGAAAG+AGG + chr7_3:198763-198782 Msa1087450:CDS 35.0%
AAAGTCTTCATCCTCATTAC+TGG - chr7_3:198984-199003 None:intergenic 35.0%
CATGCCTTCTTCTCATATGT+CGG - chr7_3:198163-198182 None:intergenic 40.0%
! GTCAATACTTTTCGACCAGT+AGG + chr7_3:198201-198220 Msa1087450:CDS 40.0%
CATCCAAGGATGTGCTTATT+TGG - chr7_3:198403-198422 None:intergenic 40.0%
AATTTGAGTGCAAGAAGGCA+TGG - chr7_3:198495-198514 None:intergenic 40.0%
TCAGGAATTTGAGTGCAAGA+AGG - chr7_3:198500-198519 None:intergenic 40.0%
TCTCCTCTTTCACAAGTACA+TGG - chr7_3:198769-198788 None:intergenic 40.0%
CTTGTGAAAGAGGAGAATCA+AGG + chr7_3:198773-198792 Msa1087450:CDS 40.0%
TGTACACAGTAATGCATCCA+AGG + chr7_3:198907-198926 Msa1087450:intron 40.0%
GTACACAGTAATGCATCCAA+GGG + chr7_3:198908-198927 Msa1087450:intron 40.0%
TCTTCATCCTCATTACTGGT+GGG - chr7_3:198980-198999 None:intergenic 40.0%
CATGCCTTCTTCTCATATGT+CGG - chr7_3:198163-198182 None:intergenic 40.0%
! GTCAATACTTTTCGACCAGT+AGG + chr7_3:198201-198220 Msa1087450:CDS 40.0%
CATCCAAGGATGTGCTTATT+TGG - chr7_3:198403-198422 None:intergenic 40.0%
AATTTGAGTGCAAGAAGGCA+TGG - chr7_3:198495-198514 None:intergenic 40.0%
TCAGGAATTTGAGTGCAAGA+AGG - chr7_3:198500-198519 None:intergenic 40.0%
TCTCCTCTTTCACAAGTACA+TGG - chr7_3:198769-198788 None:intergenic 40.0%
CTTGTGAAAGAGGAGAATCA+AGG + chr7_3:198773-198792 Msa1087450:CDS 40.0%
TGTACACAGTAATGCATCCA+AGG + chr7_3:198907-198926 Msa1087450:intron 40.0%
GTACACAGTAATGCATCCAA+GGG + chr7_3:198908-198927 Msa1087450:intron 40.0%
TCTTCATCCTCATTACTGGT+GGG - chr7_3:198980-198999 None:intergenic 40.0%
! TGTGCCGACATATGAGAAGA+AGG + chr7_3:198156-198175 Msa1087450:CDS 45.0%
TCCTTCTGTGAGAAGCGAAT+CGG - chr7_3:198321-198340 None:intergenic 45.0%
TTCTCACAGAAGGAGAAGCT+TGG + chr7_3:198327-198346 Msa1087450:CDS 45.0%
! GGGTTTTCTTGGACACCAAT+TGG + chr7_3:198367-198386 Msa1087450:CDS 45.0%
TGCCCAAATAAGCACATCCT+TGG + chr7_3:198397-198416 Msa1087450:CDS 45.0%
TACTGAACACATGGGTGACA+TGG + chr7_3:198714-198733 Msa1087450:CDS 45.0%
ATCACGGAACAAGACATGCA+AGG + chr7_3:198739-198758 Msa1087450:CDS 45.0%
CAGTAATGCATCCAAGGGAA+TGG + chr7_3:198913-198932 Msa1087450:intron 45.0%
TAATGCATCCAAGGGAATGG+TGG + chr7_3:198916-198935 Msa1087450:CDS 45.0%
GGAATGGTGGCTGATGAAAA+GGG + chr7_3:198929-198948 Msa1087450:CDS 45.0%
GTGGCTGATGAAAAGGGAAA+AGG + chr7_3:198935-198954 Msa1087450:CDS 45.0%
GTCTTCATCCTCATTACTGG+TGG - chr7_3:198981-199000 None:intergenic 45.0%
! TGTGCCGACATATGAGAAGA+AGG + chr7_3:198156-198175 Msa1087450:CDS 45.0%
TCCTTCTGTGAGAAGCGAAT+CGG - chr7_3:198321-198340 None:intergenic 45.0%
TTCTCACAGAAGGAGAAGCT+TGG + chr7_3:198327-198346 Msa1087450:CDS 45.0%
! GGGTTTTCTTGGACACCAAT+TGG + chr7_3:198367-198386 Msa1087450:CDS 45.0%
TGCCCAAATAAGCACATCCT+TGG + chr7_3:198397-198416 Msa1087450:CDS 45.0%
TACTGAACACATGGGTGACA+TGG + chr7_3:198714-198733 Msa1087450:CDS 45.0%
ATCACGGAACAAGACATGCA+AGG + chr7_3:198739-198758 Msa1087450:CDS 45.0%
CAGTAATGCATCCAAGGGAA+TGG + chr7_3:198913-198932 Msa1087450:intron 45.0%
TAATGCATCCAAGGGAATGG+TGG + chr7_3:198916-198935 Msa1087450:CDS 45.0%
GGAATGGTGGCTGATGAAAA+GGG + chr7_3:198929-198948 Msa1087450:CDS 45.0%
GTGGCTGATGAAAAGGGAAA+AGG + chr7_3:198935-198954 Msa1087450:CDS 45.0%
GTCTTCATCCTCATTACTGG+TGG - chr7_3:198981-199000 None:intergenic 45.0%
CTGAAAGTCAGCATGCCTAC+TGG - chr7_3:198219-198238 None:intergenic 50.0%
CTTGGCGAAGTAACCCAGAT+CGG + chr7_3:198345-198364 Msa1087450:CDS 50.0%
TTGGCGAAGTAACCCAGATC+GGG + chr7_3:198346-198365 Msa1087450:CDS 50.0%
! AACCCAGATCGGGGTTTTCT+TGG + chr7_3:198356-198375 Msa1087450:CDS 50.0%
GTCCAAGAAAACCCCGATCT+GGG - chr7_3:198361-198380 None:intergenic 50.0%
TGTCCAAGAAAACCCCGATC+TGG - chr7_3:198362-198381 None:intergenic 50.0%
TCTTGGACACCAATTGGAGC+TGG + chr7_3:198373-198392 Msa1087450:CDS 50.0%
CTTGGACACCAATTGGAGCT+GGG + chr7_3:198374-198393 Msa1087450:CDS 50.0%
! GTGCAAGAAGGCATGGAAGA+AGG - chr7_3:198488-198507 None:intergenic 50.0%
CATGGGTGACATGGACATCA+CGG + chr7_3:198723-198742 Msa1087450:CDS 50.0%
TTCATCAGCCACCATTCCCT+TGG - chr7_3:198927-198946 None:intergenic 50.0%
GGGAATGGTGGCTGATGAAA+AGG + chr7_3:198928-198947 Msa1087450:CDS 50.0%
ACAAGGACCCACCAGTAATG+AGG + chr7_3:198970-198989 Msa1087450:CDS 50.0%
CTGAAAGTCAGCATGCCTAC+TGG - chr7_3:198219-198238 None:intergenic 50.0%
CTTGGCGAAGTAACCCAGAT+CGG + chr7_3:198345-198364 Msa1087450:CDS 50.0%
TTGGCGAAGTAACCCAGATC+GGG + chr7_3:198346-198365 Msa1087450:CDS 50.0%
! AACCCAGATCGGGGTTTTCT+TGG + chr7_3:198356-198375 Msa1087450:CDS 50.0%
GTCCAAGAAAACCCCGATCT+GGG - chr7_3:198361-198380 None:intergenic 50.0%
TGTCCAAGAAAACCCCGATC+TGG - chr7_3:198362-198381 None:intergenic 50.0%
TCTTGGACACCAATTGGAGC+TGG + chr7_3:198373-198392 Msa1087450:CDS 50.0%
CTTGGACACCAATTGGAGCT+GGG + chr7_3:198374-198393 Msa1087450:CDS 50.0%
! GTGCAAGAAGGCATGGAAGA+AGG - chr7_3:198488-198507 None:intergenic 50.0%
CATGGGTGACATGGACATCA+CGG + chr7_3:198723-198742 Msa1087450:CDS 50.0%
TTCATCAGCCACCATTCCCT+TGG - chr7_3:198927-198946 None:intergenic 50.0%
GGGAATGGTGGCTGATGAAA+AGG + chr7_3:198928-198947 Msa1087450:CDS 50.0%
ACAAGGACCCACCAGTAATG+AGG + chr7_3:198970-198989 Msa1087450:CDS 50.0%
!! GCCGATTCGCTTCTCACAGA+AGG + chr7_3:198317-198336 Msa1087450:CDS 55.0%
TGGCGAAGTAACCCAGATCG+GGG + chr7_3:198347-198366 Msa1087450:CDS 55.0%
TTTGGGCACCCAGCTCCAAT+TGG - chr7_3:198385-198404 None:intergenic 55.0%
!! GCCGATTCGCTTCTCACAGA+AGG + chr7_3:198317-198336 Msa1087450:CDS 55.0%
TGGCGAAGTAACCCAGATCG+GGG + chr7_3:198347-198366 Msa1087450:CDS 55.0%
TTTGGGCACCCAGCTCCAAT+TGG - chr7_3:198385-198404 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 198119 199015 198119 ID=Msa1087450;Name=Msa1087450
chr7_3 mRNA 198119 199015 198119 ID=Msa1087450-mRNA-1;Parent=Msa1087450;Name=Msa1087450-mRNA-1;_AED=0.01;_eAED=0.02;_QI=0|0|0|1|1|1|3|0|200
chr7_3 exon 198119 198538 198119 ID=Msa1087450-mRNA-1:exon:79;Parent=Msa1087450-mRNA-1
chr7_3 exon 198712 198794 198712 ID=Msa1087450-mRNA-1:exon:80;Parent=Msa1087450-mRNA-1
chr7_3 exon 198916 199015 198916 ID=Msa1087450-mRNA-1:exon:81;Parent=Msa1087450-mRNA-1
chr7_3 CDS 198119 198538 198119 ID=Msa1087450-mRNA-1:cds;Parent=Msa1087450-mRNA-1
chr7_3 CDS 198712 198794 198712 ID=Msa1087450-mRNA-1:cds;Parent=Msa1087450-mRNA-1
chr7_3 CDS 198916 199015 198916 ID=Msa1087450-mRNA-1:cds;Parent=Msa1087450-mRNA-1
Gene Sequence

>Msa1087450

ATGCATCAGAGAAATACAAGGGCTATTACATGTCTTTTGTGCCGACATATGAGAAGAAGGCATGACACTGATTGTGAATTTGGTCAATACTTTTCGACCAGTAGGCATGCTGACTTTCAGTGTGTTATTCAAATTTTGGGGTTAAGTCGCCTGATGAGAATTATGCAATTAATAGAACCACATCAAAGACAAATTGTTGCCGATTCGCTTCTCACAGAAGGAGAAGCTTGGCGAAGTAACCCAGATCGGGGTTTTCTTGGACACCAATTGGAGCTGGGTGCCCAAATAAGCACATCCTTGGATGAAATTGAAATTGCTAGAAGACTTTTAGCTTTTTGCATTGATAATGCAAATTCAAACAATGCACCTTCTTCCATGCCTTCTTGCACTCAAATTCCTGACTTAAACTTGAGTATATGGAGTACTGAACACATGGGTGACATGGACATCACGGAACAAGACATGCAAGGTATTCCATGTACTTGTGAAAGAGGAGAATCAAGTAATGCATCCAAGGGAATGGTGGCTGATGAAAAGGGAAAAGGAATTCTTAATGAACAAGGACCCACCAGTAATGAGGATGAAGACTTTTCCCCAAAGTAA

Protein sequence

>Msa1087450

MHQRNTRAITCLLCRHMRRRHDTDCEFGQYFSTSRHADFQCVIQILGLSRLMRIMQLIEPHQRQIVADSLLTEGEAWRSNPDRGFLGHQLELGAQISTSLDEIEIARRLLAFCIDNANSNNAPSSMPSCTQIPDLNLSIWSTEHMGDMDITEQDMQGIPCTCERGESSNASKGMVADEKGKGILNEQGPTSNEDEDFSPK*