Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1088550 | AES61090.1 | 88.811 | 143 | 16 | 0 | 12 | 154 | 3 | 145 | 6.12e-90 | 268 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1088550 | G7I4V4 | 88.811 | 143 | 16 | 0 | 12 | 154 | 3 | 145 | 2.92e-90 | 268 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1088550 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1088550 | MtrunA17_Chr1g0187511 | 88.811 | 143 | 16 | 0 | 12 | 154 | 3 | 145 | 5.63e-94 | 268 |
| Msa1088550 | MtrunA17_Chr1g0187701 | 41.667 | 144 | 76 | 4 | 17 | 154 | 15 | 156 | 1.75e-25 | 95.5 |
| Msa1088550 | MtrunA17_Chr1g0187541 | 43.791 | 153 | 69 | 7 | 9 | 154 | 4 | 146 | 1.18e-24 | 92.8 |
| Msa1088550 | MtrunA17_Chr4g0031161 | 35.057 | 174 | 73 | 5 | 17 | 154 | 13 | 182 | 1.31e-20 | 83.2 |
| Msa1088550 | MtrunA17_Chr5g0423491 | 35.333 | 150 | 78 | 6 | 1 | 142 | 1 | 139 | 4.26e-15 | 68.2 |
| Msa1088550 | MtrunA17_Chr1g0166461 | 30.380 | 158 | 90 | 4 | 14 | 154 | 13 | 167 | 7.64e-15 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGACACATCAGTGAATT+TGG | 0.236191 | 7_3:-1794377 | Msa1088550:CDS |
| TTATCCAACATGTTGATATA+TGG | 0.260832 | 7_3:+1794433 | None:intergenic |
| GTCCCTTGTTGGTGCATTGA+TGG | 0.334927 | 7_3:+1794493 | None:intergenic |
| CCATACCACCAGTCCCTTGT+TGG | 0.378244 | 7_3:+1794482 | None:intergenic |
| ATGGGAAAATTGGGTGGTAA+AGG | 0.416030 | 7_3:+1794619 | None:intergenic |
| TAAAGGGTTGGGCATTGATT+CGG | 0.452189 | 7_3:+1794636 | None:intergenic |
| TGGGAAAATTGGGTGGTAAA+GGG | 0.468596 | 7_3:+1794620 | None:intergenic |
| CATTGATTCGGCCTGAGGAT+TGG | 0.472364 | 7_3:+1794648 | None:intergenic |
| AAGCCATCTTGAGTCTCATA+TGG | 0.473825 | 7_3:-1794707 | Msa1088550:CDS |
| CCAACAAGGGACTGGTGGTA+TGG | 0.486031 | 7_3:-1794482 | Msa1088550:CDS |
| AAATTGGGTGGTAAAGGGTT+GGG | 0.489112 | 7_3:+1794625 | None:intergenic |
| CAAAATTATTGATATTGCTA+CGG | 0.495293 | 7_3:+1794776 | None:intergenic |
| ATTCGGCCTGAGGATTGGTA+TGG | 0.511741 | 7_3:+1794653 | None:intergenic |
| AAAATTGGGTGGTAAAGGGT+TGG | 0.523418 | 7_3:+1794624 | None:intergenic |
| TTGGGCATTGATTCGGCCTG+AGG | 0.534860 | 7_3:+1794643 | None:intergenic |
| CCTTGTTGGTGCATTGATGG+TGG | 0.554951 | 7_3:+1794496 | None:intergenic |
| ATTAAACCATACCAATCCTC+AGG | 0.560036 | 7_3:-1794659 | Msa1088550:CDS |
| ACAACATTAGATTGTTGTTG+TGG | 0.560718 | 7_3:+1794541 | None:intergenic |
| ATGGAAGTCTCTCCGATCGA+TGG | 0.567201 | 7_3:-1794463 | Msa1088550:CDS |
| TAAGCCATATATCAACATGT+TGG | 0.587500 | 7_3:-1794437 | Msa1088550:CDS |
| TCAATGCACCAACAAGGGAC+TGG | 0.598187 | 7_3:-1794490 | Msa1088550:CDS |
| TTTGGCATGGGAAAATTGGG+TGG | 0.600647 | 7_3:+1794613 | None:intergenic |
| CCACCATCAATGCACCAACA+AGG | 0.600697 | 7_3:-1794496 | Msa1088550:CDS |
| ACAATCTAATGTTGTTGATG+TGG | 0.605335 | 7_3:-1794533 | Msa1088550:CDS |
| GAGCCATATGAGACTCAAGA+TGG | 0.605655 | 7_3:+1794704 | None:intergenic |
| CACCATCAATGCACCAACAA+GGG | 0.620275 | 7_3:-1794495 | Msa1088550:CDS |
| TATGGCTTAGTTCCATCGAT+CGG | 0.625287 | 7_3:+1794451 | None:intergenic |
| ATGCACCAACAAGGGACTGG+TGG | 0.640658 | 7_3:-1794487 | Msa1088550:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATCAATAATTTTGTATG+TGG | - | chr7_3:1794399-1794418 | Msa1088550:CDS | 15.0% |
| !! | CAAAATTATTGATATTGCTA+CGG | + | chr7_3:1794395-1794414 | None:intergenic | 20.0% |
| !!! | TTGTTTTCATGTAATGATTT+TGG | + | chr7_3:1794576-1794595 | None:intergenic | 20.0% |
| !!! | AAATAACTTGCTTTTAACGA+AGG | + | chr7_3:1794601-1794620 | None:intergenic | 25.0% |
| ! | TTATCCAACATGTTGATATA+TGG | + | chr7_3:1794738-1794757 | None:intergenic | 25.0% |
| !! | TCATGTAATGATTTTGGCAT+GGG | + | chr7_3:1794570-1794589 | None:intergenic | 30.0% |
| !! | TTCATGTAATGATTTTGGCA+TGG | + | chr7_3:1794571-1794590 | None:intergenic | 30.0% |
| ! | ACAACATTAGATTGTTGTTG+TGG | + | chr7_3:1794630-1794649 | None:intergenic | 30.0% |
| ACAATCTAATGTTGTTGATG+TGG | - | chr7_3:1794635-1794654 | Msa1088550:CDS | 30.0% | |
| TAAGCCATATATCAACATGT+TGG | - | chr7_3:1794731-1794750 | Msa1088550:CDS | 30.0% | |
| ! | ATGAAGACATTTTTCTCGAT+GGG | + | chr7_3:1794762-1794781 | None:intergenic | 30.0% |
| ! | AATGAAGACATTTTTCTCGA+TGG | + | chr7_3:1794763-1794782 | None:intergenic | 30.0% |
| ATTAAACCATACCAATCCTC+AGG | - | chr7_3:1794509-1794528 | Msa1088550:CDS | 35.0% | |
| !! | GATTTTGGCATGGGAAAATT+GGG | + | chr7_3:1794561-1794580 | None:intergenic | 35.0% |
| !! | TGATTTTGGCATGGGAAAAT+TGG | + | chr7_3:1794562-1794581 | None:intergenic | 35.0% |
| CAATGACACATCAGTGAATT+TGG | - | chr7_3:1794791-1794810 | Msa1088550:CDS | 35.0% | |
| AAGCCATCTTGAGTCTCATA+TGG | - | chr7_3:1794461-1794480 | Msa1088550:CDS | 40.0% | |
| !! | TAAAGGGTTGGGCATTGATT+CGG | + | chr7_3:1794535-1794554 | None:intergenic | 40.0% |
| AAATTGGGTGGTAAAGGGTT+GGG | + | chr7_3:1794546-1794565 | None:intergenic | 40.0% | |
| AAAATTGGGTGGTAAAGGGT+TGG | + | chr7_3:1794547-1794566 | None:intergenic | 40.0% | |
| TGGGAAAATTGGGTGGTAAA+GGG | + | chr7_3:1794551-1794570 | None:intergenic | 40.0% | |
| ATGGGAAAATTGGGTGGTAA+AGG | + | chr7_3:1794552-1794571 | None:intergenic | 40.0% | |
| TATGGCTTAGTTCCATCGAT+CGG | + | chr7_3:1794720-1794739 | None:intergenic | 40.0% | |
| GAGCCATATGAGACTCAAGA+TGG | + | chr7_3:1794467-1794486 | None:intergenic | 45.0% | |
| TTTGGCATGGGAAAATTGGG+TGG | + | chr7_3:1794558-1794577 | None:intergenic | 45.0% | |
| CACCATCAATGCACCAACAA+GGG | - | chr7_3:1794673-1794692 | Msa1088550:CDS | 45.0% | |
| ! | ATTCGGCCTGAGGATTGGTA+TGG | + | chr7_3:1794518-1794537 | None:intergenic | 50.0% |
| !! | CATTGATTCGGCCTGAGGAT+TGG | + | chr7_3:1794523-1794542 | None:intergenic | 50.0% |
| ! | CCTTGTTGGTGCATTGATGG+TGG | + | chr7_3:1794675-1794694 | None:intergenic | 50.0% |
| CCACCATCAATGCACCAACA+AGG | - | chr7_3:1794672-1794691 | Msa1088550:CDS | 50.0% | |
| GTCCCTTGTTGGTGCATTGA+TGG | + | chr7_3:1794678-1794697 | None:intergenic | 50.0% | |
| TCAATGCACCAACAAGGGAC+TGG | - | chr7_3:1794678-1794697 | Msa1088550:CDS | 50.0% | |
| ATGGAAGTCTCTCCGATCGA+TGG | - | chr7_3:1794705-1794724 | Msa1088550:CDS | 50.0% | |
| !! | TTGGGCATTGATTCGGCCTG+AGG | + | chr7_3:1794528-1794547 | None:intergenic | 55.0% |
| ATGCACCAACAAGGGACTGG+TGG | - | chr7_3:1794681-1794700 | Msa1088550:CDS | 55.0% | |
| CCATACCACCAGTCCCTTGT+TGG | + | chr7_3:1794689-1794708 | None:intergenic | 55.0% | |
| ! | CCAACAAGGGACTGGTGGTA+TGG | - | chr7_3:1794686-1794705 | Msa1088550:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 1794363 | 1794827 | 1794363 | ID=Msa1088550;Name=Msa1088550 |
| chr7_3 | mRNA | 1794363 | 1794827 | 1794363 | ID=Msa1088550-mRNA-1;Parent=Msa1088550;Name=Msa1088550-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|154 |
| chr7_3 | exon | 1794363 | 1794827 | 1794363 | ID=Msa1088550-mRNA-1:exon:696;Parent=Msa1088550-mRNA-1 |
| chr7_3 | CDS | 1794363 | 1794827 | 1794363 | ID=Msa1088550-mRNA-1:cds;Parent=Msa1088550-mRNA-1 |
| Gene Sequence |
| Protein sequence |
