Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1090710 | XP_013447966.1 | 89.545 | 220 | 23 | 0 | 1 | 220 | 1 | 220 | 1.25e-139 | 399 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1090710 | A0A072TYR6 | 89.545 | 220 | 23 | 0 | 1 | 220 | 1 | 220 | 5.98e-140 | 399 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1090710 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1090710 | MtrunA17_Chr7g0225091 | 89.545 | 220 | 23 | 0 | 1 | 220 | 1 | 220 | 1.15e-143 | 399 |
| Msa1090710 | MtrunA17_Chr7g0255511 | 81.197 | 234 | 30 | 1 | 1 | 220 | 1 | 234 | 3.35e-131 | 369 |
| Msa1090710 | MtrunA17_Chr7g0225121 | 71.795 | 234 | 30 | 2 | 1 | 220 | 1 | 212 | 1.67e-112 | 320 |
| Msa1090710 | MtrunA17_Chr7g0222361 | 74.257 | 202 | 37 | 1 | 34 | 220 | 1 | 202 | 1.42e-106 | 305 |
| Msa1090710 | MtrunA17_Chr7g0222371 | 66.942 | 121 | 25 | 1 | 19 | 124 | 1 | 121 | 3.66e-52 | 164 |
| Msa1090710 | MtrunA17_Chr4g0072571 | 30.714 | 140 | 78 | 4 | 83 | 220 | 47 | 169 | 6.83e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAATGGAGAAATTTCTT+TGG | 0.293893 | 7_3:-5657850 | None:intergenic |
| CTCCTTCCTCTTGTTGCACA+TGG | 0.338528 | 7_3:-5657670 | None:intergenic |
| TATATATAAATCAAAGCTTA+AGG | 0.356106 | 7_3:-5658322 | None:intergenic |
| GGGTGTGGATATCAATATAA+TGG | 0.359691 | 7_3:+5658056 | Msa1090710:CDS |
| TGGTGCATTGCAACATATTC+CGG | 0.362580 | 7_3:+5658136 | Msa1090710:CDS |
| CACCATCACTCCATTTCATA+TGG | 0.364608 | 7_3:+5658034 | Msa1090710:CDS |
| CTTAAGGGTCAAATCAATCT+TGG | 0.396784 | 7_3:-5658306 | None:intergenic |
| AACAATATGATTCAACATGC+AGG | 0.449556 | 7_3:+5658083 | Msa1090710:CDS |
| TATCCACACCCCATATGAAA+TGG | 0.452870 | 7_3:-5658044 | None:intergenic |
| AGTAGACAATTTGAAGATGA+TGG | 0.466141 | 7_3:+5657710 | Msa1090710:CDS |
| TTCACTAGTCCACAGGCATT+AGG | 0.474635 | 7_3:+5657945 | Msa1090710:CDS |
| TTGGATTCATTGTTGATCAC+AGG | 0.479894 | 7_3:+5657877 | Msa1090710:CDS |
| TGAAGTTGGTAAAGTTTCAG+AGG | 0.484639 | 7_3:+5658223 | Msa1090710:CDS |
| ACTATGTCAAAGATTGAAGT+TGG | 0.488225 | 7_3:+5658209 | Msa1090710:CDS |
| ACCATCACTCCATTTCATAT+GGG | 0.489513 | 7_3:+5658035 | Msa1090710:CDS |
| TGGTGTTTAGACTTATCCCT+TGG | 0.491881 | 7_3:-5658016 | None:intergenic |
| TGTTAGTGATGAAGTTGATC+AGG | 0.506770 | 7_3:+5658253 | Msa1090710:CDS |
| CAAGCGAAAGGCAATTGTGC+TGG | 0.510108 | 7_3:+5657752 | Msa1090710:CDS |
| GAAGTTGGTAAAGTTTCAGA+GGG | 0.513957 | 7_3:+5658224 | Msa1090710:CDS |
| ATATATAAATCAAAGCTTAA+GGG | 0.520066 | 7_3:-5658321 | None:intergenic |
| ACTCCATTTCATATGGGGTG+TGG | 0.533278 | 7_3:+5658041 | Msa1090710:CDS |
| GGAGATAGATGAAGCGAATG+AGG | 0.566081 | 7_3:+5658157 | Msa1090710:CDS |
| TCATTCGCTTCATCTATCTC+CGG | 0.569516 | 7_3:-5658155 | None:intergenic |
| CAGAAAGTTCACTAGTCCAC+AGG | 0.575345 | 7_3:+5657938 | Msa1090710:CDS |
| ACAATATGATTCAACATGCA+GGG | 0.581032 | 7_3:+5658084 | Msa1090710:CDS |
| CTGATGCCATGTGCAACAAG+AGG | 0.598560 | 7_3:+5657664 | None:intergenic |
| TAGAATTCTGAGTTTCAACA+TGG | 0.602831 | 7_3:-5657782 | None:intergenic |
| TGCCATGTGCAACAAGAGGA+AGG | 0.602996 | 7_3:+5657668 | None:intergenic |
| GTTGGTAAAGTTTCAGAGGG+AGG | 0.624522 | 7_3:+5658227 | Msa1090710:CDS |
| CCATCACTCCATTTCATATG+GGG | 0.630153 | 7_3:+5658036 | Msa1090710:CDS |
| GGAAGACAAAGAGACTCAAG+AGG | 0.634668 | 7_3:+5658274 | Msa1090710:CDS |
| GTGCAACAAGAGGAAGGAGA+AGG | 0.635140 | 7_3:+5657674 | Msa1090710:CDS |
| CCCCATATGAAATGGAGTGA+TGG | 0.640487 | 7_3:-5658036 | None:intergenic |
| GAACAATGAGAACAAGCGAA+AGG | 0.645636 | 7_3:+5657740 | Msa1090710:CDS |
| ACTAGTCCACAGGCATTAGG+TGG | 0.661075 | 7_3:+5657948 | Msa1090710:CDS |
| TGATGACCACCTAATGCCTG+TGG | 0.664402 | 7_3:-5657954 | None:intergenic |
| AGAATTCTGAGTTTCAACAT+GGG | 0.673130 | 7_3:-5657781 | None:intergenic |
| AGACAAAGAGACTCAAGAGG+AGG | 0.673262 | 7_3:+5658277 | Msa1090710:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAAATGGAGAAATTTCTT+TGG | - | chr7_3:5657853-5657872 | None:intergenic | 25.0% |
| !! | ATTTCTCCATTTCTTCTTTT+TGG | + | chr7_3:5657858-5657877 | Msa1090710:CDS | 25.0% |
| ! | ATGAATCCAAAAAGAAGAAA+TGG | - | chr7_3:5657867-5657886 | None:intergenic | 25.0% |
| !! | TTTTATGCAGGAAATTTGTA+TGG | + | chr7_3:5658116-5658135 | Msa1090710:CDS | 25.0% |
| AGTAGACAATTTGAAGATGA+TGG | + | chr7_3:5657710-5657729 | Msa1090710:CDS | 30.0% | |
| AGAATTCTGAGTTTCAACAT+GGG | - | chr7_3:5657784-5657803 | None:intergenic | 30.0% | |
| TAGAATTCTGAGTTTCAACA+TGG | - | chr7_3:5657785-5657804 | None:intergenic | 30.0% | |
| AGTCTCAATAAAAAGATCCA+AGG | + | chr7_3:5657999-5658018 | Msa1090710:CDS | 30.0% | |
| GTCTCAATAAAAAGATCCAA+GGG | + | chr7_3:5658000-5658019 | Msa1090710:CDS | 30.0% | |
| AACAATATGATTCAACATGC+AGG | + | chr7_3:5658083-5658102 | Msa1090710:CDS | 30.0% | |
| ACAATATGATTCAACATGCA+GGG | + | chr7_3:5658084-5658103 | Msa1090710:CDS | 30.0% | |
| ACTATGTCAAAGATTGAAGT+TGG | + | chr7_3:5658209-5658228 | Msa1090710:CDS | 30.0% | |
| ! | CTTCTTCAGCAACATCTTTT+TGG | - | chr7_3:5657815-5657834 | None:intergenic | 35.0% |
| TTGGATTCATTGTTGATCAC+AGG | + | chr7_3:5657877-5657896 | Msa1090710:CDS | 35.0% | |
| ACCATCACTCCATTTCATAT+GGG | + | chr7_3:5658035-5658054 | Msa1090710:CDS | 35.0% | |
| GGGTGTGGATATCAATATAA+TGG | + | chr7_3:5658056-5658075 | Msa1090710:CDS | 35.0% | |
| !! | TGAAGTTGGTAAAGTTTCAG+AGG | + | chr7_3:5658223-5658242 | Msa1090710:CDS | 35.0% |
| !! | GAAGTTGGTAAAGTTTCAGA+GGG | + | chr7_3:5658224-5658243 | Msa1090710:CDS | 35.0% |
| ! | TGTTAGTGATGAAGTTGATC+AGG | + | chr7_3:5658253-5658272 | Msa1090710:CDS | 35.0% |
| CTTAAGGGTCAAATCAATCT+TGG | - | chr7_3:5658309-5658328 | None:intergenic | 35.0% | |
| GAACAATGAGAACAAGCGAA+AGG | + | chr7_3:5657740-5657759 | Msa1090710:CDS | 40.0% | |
| TGGTGTTTAGACTTATCCCT+TGG | - | chr7_3:5658019-5658038 | None:intergenic | 40.0% | |
| CACCATCACTCCATTTCATA+TGG | + | chr7_3:5658034-5658053 | Msa1090710:CDS | 40.0% | |
| CCATCACTCCATTTCATATG+GGG | + | chr7_3:5658036-5658055 | Msa1090710:CDS | 40.0% | |
| TATCCACACCCCATATGAAA+TGG | - | chr7_3:5658047-5658066 | None:intergenic | 40.0% | |
| ! | GGGTCATCAAACTTTTATGC+AGG | + | chr7_3:5658104-5658123 | Msa1090710:CDS | 40.0% |
| TGGTGCATTGCAACATATTC+CGG | + | chr7_3:5658136-5658155 | Msa1090710:CDS | 40.0% | |
| TCATTCGCTTCATCTATCTC+CGG | - | chr7_3:5658158-5658177 | None:intergenic | 40.0% | |
| CAGAAAGTTCACTAGTCCAC+AGG | + | chr7_3:5657938-5657957 | Msa1090710:CDS | 45.0% | |
| TTCACTAGTCCACAGGCATT+AGG | + | chr7_3:5657945-5657964 | Msa1090710:CDS | 45.0% | |
| CCCCATATGAAATGGAGTGA+TGG | - | chr7_3:5658039-5658058 | None:intergenic | 45.0% | |
| ACTCCATTTCATATGGGGTG+TGG | + | chr7_3:5658041-5658060 | Msa1090710:CDS | 45.0% | |
| GGAGATAGATGAAGCGAATG+AGG | + | chr7_3:5658157-5658176 | Msa1090710:CDS | 45.0% | |
| ! | GTTGGTAAAGTTTCAGAGGG+AGG | + | chr7_3:5658227-5658246 | Msa1090710:CDS | 45.0% |
| GGAAGACAAAGAGACTCAAG+AGG | + | chr7_3:5658274-5658293 | Msa1090710:CDS | 45.0% | |
| AGACAAAGAGACTCAAGAGG+AGG | + | chr7_3:5658277-5658296 | Msa1090710:CDS | 45.0% | |
| GTGCAACAAGAGGAAGGAGA+AGG | + | chr7_3:5657674-5657693 | Msa1090710:CDS | 50.0% | |
| CAAGCGAAAGGCAATTGTGC+TGG | + | chr7_3:5657752-5657771 | Msa1090710:CDS | 50.0% | |
| ACTAGTCCACAGGCATTAGG+TGG | + | chr7_3:5657948-5657967 | Msa1090710:CDS | 50.0% | |
| TGATGACCACCTAATGCCTG+TGG | - | chr7_3:5657957-5657976 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 5657672 | 5658334 | 5657672 | ID=Msa1090710;Name=Msa1090710 |
| chr7_3 | mRNA | 5657672 | 5658334 | 5657672 | ID=Msa1090710-mRNA-1;Parent=Msa1090710;Name=Msa1090710-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|220 |
| chr7_3 | exon | 5657672 | 5658334 | 5657672 | ID=Msa1090710-mRNA-1:exon:1720;Parent=Msa1090710-mRNA-1 |
| chr7_3 | CDS | 5657672 | 5658334 | 5657672 | ID=Msa1090710-mRNA-1:cds;Parent=Msa1090710-mRNA-1 |
| Gene Sequence |
| Protein sequence |
