Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1092740 | XP_039688063.1 | 86.184 | 152 | 20 | 1 | 1 | 151 | 22 | 173 | 1.94e-87 | 273 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1092740 | A0A072U9Z0 | 76.871 | 147 | 22 | 3 | 4 | 149 | 48 | 183 | 2.16e-70 | 220 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1092740 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1092740 | MtrunA17_Chr4g0033611 | 36.029 | 136 | 71 | 5 | 26 | 155 | 61 | 186 | 4.11e-18 | 78.2 |
| Msa1092740 | MtrunA17_Chr2g0329421 | 34.307 | 137 | 79 | 3 | 16 | 150 | 1 | 128 | 2.76e-16 | 72.8 |
| Msa1092740 | MtrunA17_Chr7g0233911 | 37.069 | 116 | 58 | 4 | 41 | 151 | 33 | 138 | 5.91e-14 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1092740 | AT3G59470.3 | 37.838 | 111 | 58 | 3 | 41 | 149 | 69 | 170 | 1.72e-13 | 66.2 |
| Msa1092740 | AT3G59470.1 | 37.838 | 111 | 58 | 3 | 41 | 149 | 69 | 170 | 1.72e-13 | 66.2 |
| Msa1092740 | AT3G59470.2 | 37.838 | 111 | 58 | 3 | 41 | 149 | 81 | 182 | 2.19e-13 | 65.9 |
| Msa1092740 | AT3G07500.1 | 31.618 | 136 | 74 | 5 | 26 | 149 | 6 | 134 | 4.10e-12 | 62.0 |
Find 19 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTACCATCCTTCTTATT+TGG | 0.212526 | 7_3:+10380695 | None:intergenic |
| TTTAATTCATCATCACAATT+TGG | 0.253101 | 7_3:+10380881 | None:intergenic |
| CCAAATACATCAGTTTGAAT+TGG | 0.335509 | 7_3:-10381432 | None:intergenic |
| ACTTCAAGAGACCGTTTCTA+AGG | 0.391113 | 7_3:-10380533 | None:intergenic |
| TCAAATACTTGTCCAATCAT+AGG | 0.403178 | 7_3:+10380857 | None:intergenic |
| GATGAATTAAAGCCTATGAT+TGG | 0.477938 | 7_3:-10380869 | Msa1092740:CDS |
| GGGTTATTTGCCAAATAAGA+AGG | 0.490320 | 7_3:-10380705 | Msa1092740:CDS |
| CTGTCAAGGTTAGAAGAAGA+AGG | 0.491761 | 7_3:-10380658 | Msa1092740:CDS |
| GCCAAATAAGAAGGATGGTA+AGG | 0.493986 | 7_3:-10380696 | Msa1092740:CDS |
| TGAGTTACGTTCATTCTCCA+TGG | 0.497242 | 7_3:-10381392 | Msa1092740:CDS |
| AAACGGTCTCTTGAAGTAAG+TGG | 0.510860 | 7_3:+10380539 | None:intergenic |
| TTGAAGTAAGTGGATGTGTA+TGG | 0.512376 | 7_3:+10380549 | None:intergenic |
| TTCCTTGTAGACCTTAGAAA+CGG | 0.517176 | 7_3:+10380522 | None:intergenic |
| AGTTGATGTATCTTTGTCCA+TGG | 0.526717 | 7_3:+10381375 | None:intergenic |
| AGAAGGGCTTTAACAAGAGA+AGG | 0.550276 | 7_3:-10380641 | Msa1092740:CDS |
| TATTTGCCAAATAAGAAGGA+TGG | 0.592394 | 7_3:-10380701 | Msa1092740:CDS |
| GCAAAGTGAATCTACTGTCA+AGG | 0.595204 | 7_3:-10380672 | Msa1092740:CDS |
| TGTCAAGGTTAGAAGAAGAA+GGG | 0.596214 | 7_3:-10380657 | Msa1092740:CDS |
| GTATTTGATATGTTAGCAGA+AGG | 0.597215 | 7_3:-10380842 | Msa1092740:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GGAAAACATAATAAATAAAA+AGG | + | chr7_3:10380995-10381014 | None:intergenic | 15.0% |
| !! | TCTTCAATAAAATAATAGAA+AGG | + | chr7_3:10381016-10381035 | None:intergenic | 15.0% |
| !! | GGAAAACATAATAAATAAAA+AGG | + | chr7_3:10380995-10381014 | None:intergenic | 15.0% |
| !! | TCTTCAATAAAATAATAGAA+AGG | + | chr7_3:10381016-10381035 | None:intergenic | 15.0% |
| !! | AACTAATTATAATATACCTG+AGG | + | chr7_3:10380616-10380635 | None:intergenic | 20.0% |
| !! | CAGGTATATTATAATTAGTT+TGG | - | chr7_3:10380616-10380635 | Msa1092740:CDS | 20.0% |
| !!! | TTTTGTTCCTATTTTTTTGT+TGG | - | chr7_3:10380703-10380722 | Msa1092740:CDS | 20.0% |
| !! | CCAAACTAAGAAAAAAAAAT+AGG | + | chr7_3:10380831-10380850 | None:intergenic | 20.0% |
| !!! | CCTATTTTTTTTTCTTAGTT+TGG | - | chr7_3:10380828-10380847 | Msa1092740:CDS | 20.0% |
| !! | TTTAATTCATCATCACAATT+TGG | + | chr7_3:10381075-10381094 | None:intergenic | 20.0% |
| !! | AACTAATTATAATATACCTG+AGG | + | chr7_3:10380616-10380635 | None:intergenic | 20.0% |
| !! | CAGGTATATTATAATTAGTT+TGG | - | chr7_3:10380616-10380635 | Msa1092740:CDS | 20.0% |
| !!! | TTTTGTTCCTATTTTTTTGT+TGG | - | chr7_3:10380703-10380722 | Msa1092740:CDS | 20.0% |
| !! | CCAAACTAAGAAAAAAAAAT+AGG | + | chr7_3:10380831-10380850 | None:intergenic | 20.0% |
| !!! | CCTATTTTTTTTTCTTAGTT+TGG | - | chr7_3:10380828-10380847 | Msa1092740:CDS | 20.0% |
| !! | TTTAATTCATCATCACAATT+TGG | + | chr7_3:10381075-10381094 | None:intergenic | 20.0% |
| !!! | ATTATAATTAGTTTGGCTTG+AGG | - | chr7_3:10380623-10380642 | Msa1092740:CDS | 25.0% |
| ! | ACAAAAAAAACCTATCTCTT+AGG | + | chr7_3:10380689-10380708 | None:intergenic | 25.0% |
| !! | TCATCGACTATAGATAAAAA+CGG | - | chr7_3:10381189-10381208 | Msa1092740:intron | 25.0% |
| !!! | ATGCAAATGTTGTTTTCAAA+TGG | - | chr7_3:10381340-10381359 | Msa1092740:intron | 25.0% |
| !!! | TGCAAATGTTGTTTTCAAAT+GGG | - | chr7_3:10381341-10381360 | Msa1092740:intron | 25.0% |
| !!! | ATTATAATTAGTTTGGCTTG+AGG | - | chr7_3:10380623-10380642 | Msa1092740:CDS | 25.0% |
| ! | ACAAAAAAAACCTATCTCTT+AGG | + | chr7_3:10380689-10380708 | None:intergenic | 25.0% |
| !! | TCATCGACTATAGATAAAAA+CGG | - | chr7_3:10381189-10381208 | Msa1092740:intron | 25.0% |
| !!! | ATGCAAATGTTGTTTTCAAA+TGG | - | chr7_3:10381340-10381359 | Msa1092740:intron | 25.0% |
| !!! | TGCAAATGTTGTTTTCAAAT+GGG | - | chr7_3:10381341-10381360 | Msa1092740:intron | 25.0% |
| !! | AACTTGTTTTCAAATTCCTC+AGG | - | chr7_3:10380597-10380616 | Msa1092740:CDS | 30.0% |
| !!! | TTGCTTTTTTCCTAAGAGAT+AGG | - | chr7_3:10380676-10380695 | Msa1092740:CDS | 30.0% |
| AGTGGAACCAACAAAAAAAT+AGG | + | chr7_3:10380713-10380732 | None:intergenic | 30.0% | |
| GATGAATTAAAGCCTATGAT+TGG | - | chr7_3:10381084-10381103 | Msa1092740:intron | 30.0% | |
| TCAAATACTTGTCCAATCAT+AGG | + | chr7_3:10381099-10381118 | None:intergenic | 30.0% | |
| GTATTTGATATGTTAGCAGA+AGG | - | chr7_3:10381111-10381130 | Msa1092740:intron | 30.0% | |
| !! | TATTTTGTTTGCTCGAAAGA+GGG | - | chr7_3:10381228-10381247 | Msa1092740:intron | 30.0% |
| TATTTGCCAAATAAGAAGGA+TGG | - | chr7_3:10381252-10381271 | Msa1092740:intron | 30.0% | |
| !!! | AAATGTTGTTTTCAAATGGG+TGG | - | chr7_3:10381344-10381363 | Msa1092740:intron | 30.0% |
| !!! | AATGTTGTTTTCAAATGGGT+GGG | - | chr7_3:10381345-10381364 | Msa1092740:intron | 30.0% |
| !! | AACTTGTTTTCAAATTCCTC+AGG | - | chr7_3:10380597-10380616 | Msa1092740:CDS | 30.0% |
| !!! | TTGCTTTTTTCCTAAGAGAT+AGG | - | chr7_3:10380676-10380695 | Msa1092740:CDS | 30.0% |
| AGTGGAACCAACAAAAAAAT+AGG | + | chr7_3:10380713-10380732 | None:intergenic | 30.0% | |
| GATGAATTAAAGCCTATGAT+TGG | - | chr7_3:10381084-10381103 | Msa1092740:intron | 30.0% | |
| TCAAATACTTGTCCAATCAT+AGG | + | chr7_3:10381099-10381118 | None:intergenic | 30.0% | |
| GTATTTGATATGTTAGCAGA+AGG | - | chr7_3:10381111-10381130 | Msa1092740:intron | 30.0% | |
| !! | TATTTTGTTTGCTCGAAAGA+GGG | - | chr7_3:10381228-10381247 | Msa1092740:intron | 30.0% |
| TATTTGCCAAATAAGAAGGA+TGG | - | chr7_3:10381252-10381271 | Msa1092740:intron | 30.0% | |
| !!! | AAATGTTGTTTTCAAATGGG+TGG | - | chr7_3:10381344-10381363 | Msa1092740:intron | 30.0% |
| !!! | AATGTTGTTTTCAAATGGGT+GGG | - | chr7_3:10381345-10381364 | Msa1092740:intron | 30.0% |
| ! | AGTTGATGTATCTTTGTCCA+TGG | + | chr7_3:10380581-10380600 | None:intergenic | 35.0% |
| AACCACCTAAGATTAATGCA+GGG | + | chr7_3:10380889-10380908 | None:intergenic | 35.0% | |
| AAACCACCTAAGATTAATGC+AGG | + | chr7_3:10380890-10380909 | None:intergenic | 35.0% | |
| !! | GTATTTTGTTTGCTCGAAAG+AGG | - | chr7_3:10381227-10381246 | Msa1092740:intron | 35.0% |
| GGGTTATTTGCCAAATAAGA+AGG | - | chr7_3:10381248-10381267 | Msa1092740:intron | 35.0% | |
| TCCTTACCATCCTTCTTATT+TGG | + | chr7_3:10381261-10381280 | None:intergenic | 35.0% | |
| TGTCAAGGTTAGAAGAAGAA+GGG | - | chr7_3:10381296-10381315 | Msa1092740:intron | 35.0% | |
| !!! | ATGTTGTTTTCAAATGGGTG+GGG | - | chr7_3:10381346-10381365 | Msa1092740:intron | 35.0% |
| TTGAAGTAAGTGGATGTGTA+TGG | + | chr7_3:10381407-10381426 | None:intergenic | 35.0% | |
| ! | AGTTGATGTATCTTTGTCCA+TGG | + | chr7_3:10380581-10380600 | None:intergenic | 35.0% |
| AACCACCTAAGATTAATGCA+GGG | + | chr7_3:10380889-10380908 | None:intergenic | 35.0% | |
| AAACCACCTAAGATTAATGC+AGG | + | chr7_3:10380890-10380909 | None:intergenic | 35.0% | |
| !! | GTATTTTGTTTGCTCGAAAG+AGG | - | chr7_3:10381227-10381246 | Msa1092740:intron | 35.0% |
| GGGTTATTTGCCAAATAAGA+AGG | - | chr7_3:10381248-10381267 | Msa1092740:intron | 35.0% | |
| TCCTTACCATCCTTCTTATT+TGG | + | chr7_3:10381261-10381280 | None:intergenic | 35.0% | |
| TGTCAAGGTTAGAAGAAGAA+GGG | - | chr7_3:10381296-10381315 | Msa1092740:intron | 35.0% | |
| !!! | ATGTTGTTTTCAAATGGGTG+GGG | - | chr7_3:10381346-10381365 | Msa1092740:intron | 35.0% |
| TTGAAGTAAGTGGATGTGTA+TGG | + | chr7_3:10381407-10381426 | None:intergenic | 35.0% | |
| TGAGTTACGTTCATTCTCCA+TGG | - | chr7_3:10380561-10380580 | Msa1092740:CDS | 40.0% | |
| CAACAACCAAATAGAACCAC+GGG | + | chr7_3:10380741-10380760 | None:intergenic | 40.0% | |
| CCAACAACCAAATAGAACCA+CGG | + | chr7_3:10380742-10380761 | None:intergenic | 40.0% | |
| AGAAGCCCTGCATTAATCTT+AGG | - | chr7_3:10380881-10380900 | Msa1092740:CDS | 40.0% | |
| GAAAAATACGCACTCTCTGT+CGG | - | chr7_3:10381147-10381166 | Msa1092740:intron | 40.0% | |
| ATAAAAACGGTGTGAAGCGT+TGG | - | chr7_3:10381202-10381221 | Msa1092740:intron | 40.0% | |
| GCCAAATAAGAAGGATGGTA+AGG | - | chr7_3:10381257-10381276 | Msa1092740:intron | 40.0% | |
| GCAAAGTGAATCTACTGTCA+AGG | - | chr7_3:10381281-10381300 | Msa1092740:intron | 40.0% | |
| CTGTCAAGGTTAGAAGAAGA+AGG | - | chr7_3:10381295-10381314 | Msa1092740:intron | 40.0% | |
| ! | AGAAGGGCTTTAACAAGAGA+AGG | - | chr7_3:10381312-10381331 | Msa1092740:intron | 40.0% |
| !!! | TGTTGTTTTCAAATGGGTGG+GGG | - | chr7_3:10381347-10381366 | Msa1092740:intron | 40.0% |
| AAACGGTCTCTTGAAGTAAG+TGG | + | chr7_3:10381417-10381436 | None:intergenic | 40.0% | |
| TGAGTTACGTTCATTCTCCA+TGG | - | chr7_3:10380561-10380580 | Msa1092740:CDS | 40.0% | |
| CAACAACCAAATAGAACCAC+GGG | + | chr7_3:10380741-10380760 | None:intergenic | 40.0% | |
| CCAACAACCAAATAGAACCA+CGG | + | chr7_3:10380742-10380761 | None:intergenic | 40.0% | |
| AGAAGCCCTGCATTAATCTT+AGG | - | chr7_3:10380881-10380900 | Msa1092740:CDS | 40.0% | |
| GAAAAATACGCACTCTCTGT+CGG | - | chr7_3:10381147-10381166 | Msa1092740:intron | 40.0% | |
| ATAAAAACGGTGTGAAGCGT+TGG | - | chr7_3:10381202-10381221 | Msa1092740:intron | 40.0% | |
| GCCAAATAAGAAGGATGGTA+AGG | - | chr7_3:10381257-10381276 | Msa1092740:intron | 40.0% | |
| GCAAAGTGAATCTACTGTCA+AGG | - | chr7_3:10381281-10381300 | Msa1092740:intron | 40.0% | |
| CTGTCAAGGTTAGAAGAAGA+AGG | - | chr7_3:10381295-10381314 | Msa1092740:intron | 40.0% | |
| ! | AGAAGGGCTTTAACAAGAGA+AGG | - | chr7_3:10381312-10381331 | Msa1092740:intron | 40.0% |
| !!! | TGTTGTTTTCAAATGGGTGG+GGG | - | chr7_3:10381347-10381366 | Msa1092740:intron | 40.0% |
| AAACGGTCTCTTGAAGTAAG+TGG | + | chr7_3:10381417-10381436 | None:intergenic | 40.0% | |
| ! | CCGTGGTTCTATTTGGTTGT+TGG | - | chr7_3:10380739-10380758 | Msa1092740:CDS | 45.0% |
| AGCCCTGCATTAATCTTAGG+TGG | - | chr7_3:10380884-10380903 | Msa1092740:CDS | 45.0% | |
| ! | CCGTGGTTCTATTTGGTTGT+TGG | - | chr7_3:10380739-10380758 | Msa1092740:CDS | 45.0% |
| AGCCCTGCATTAATCTTAGG+TGG | - | chr7_3:10380884-10380903 | Msa1092740:CDS | 45.0% | |
| !!! | ATATTTATTTTGTTAATTTT+AGG | - | chr7_3:10381035-10381054 | Msa1092740:intron | 5.0% |
| !!! | ATATTTATTTTGTTAATTTT+AGG | - | chr7_3:10381035-10381054 | Msa1092740:intron | 5.0% |
| ! | TGCTGTCCCGTGGTTCTATT+TGG | - | chr7_3:10380732-10380751 | Msa1092740:CDS | 50.0% |
| ! | GTTTTCAAATGGGTGGGGGA+AGG | - | chr7_3:10381351-10381370 | Msa1092740:intron | 50.0% |
| ! | TGCTGTCCCGTGGTTCTATT+TGG | - | chr7_3:10380732-10380751 | Msa1092740:CDS | 50.0% |
| ! | GTTTTCAAATGGGTGGGGGA+AGG | - | chr7_3:10381351-10381370 | Msa1092740:intron | 50.0% |
| ATAGAACCACGGGACAGCAG+TGG | + | chr7_3:10380731-10380750 | None:intergenic | 55.0% | |
| ATAGAACCACGGGACAGCAG+TGG | + | chr7_3:10380731-10380750 | None:intergenic | 55.0% | |
| TTGGTTCCACTGCTGTCCCG+TGG | - | chr7_3:10380722-10380741 | Msa1092740:CDS | 60.0% | |
| TTGGTTCCACTGCTGTCCCG+TGG | - | chr7_3:10380722-10380741 | Msa1092740:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 10380535 | 10381440 | 10380535 | ID=Msa1092740;Name=Msa1092740 |
| chr7_3 | mRNA | 10380535 | 10381440 | 10380535 | ID=Msa1092740-mRNA-1;Parent=Msa1092740;Name=Msa1092740-mRNA-1;_AED=0.36;_eAED=0.38;_QI=0|0|0|1|1|1|2|0|155 |
| chr7_3 | exon | 10381357 | 10381440 | 10381357 | ID=Msa1092740-mRNA-1:exon:2768;Parent=Msa1092740-mRNA-1 |
| chr7_3 | exon | 10380535 | 10380918 | 10380535 | ID=Msa1092740-mRNA-1:exon:2767;Parent=Msa1092740-mRNA-1 |
| chr7_3 | CDS | 10381357 | 10381440 | 10381357 | ID=Msa1092740-mRNA-1:cds;Parent=Msa1092740-mRNA-1 |
| chr7_3 | CDS | 10380535 | 10380918 | 10380535 | ID=Msa1092740-mRNA-1:cds;Parent=Msa1092740-mRNA-1 |
| Gene Sequence |
| Protein sequence |
