Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1092750 | XP_039688063.1 | 87.500 | 136 | 17 | 0 | 1 | 136 | 38 | 173 | 1.05e-80 | 255 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1092750 | A0A072U9Z0 | 77.612 | 134 | 19 | 2 | 1 | 134 | 61 | 183 | 1.65e-66 | 209 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1092750 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1092750 | MtrunA17_Chr4g0033611 | 36.029 | 136 | 71 | 5 | 11 | 140 | 61 | 186 | 7.43e-19 | 79.7 |
| Msa1092750 | MtrunA17_Chr2g0329421 | 34.307 | 137 | 79 | 3 | 1 | 135 | 1 | 128 | 4.97e-17 | 73.9 |
| Msa1092750 | MtrunA17_Chr7g0233911 | 37.069 | 116 | 58 | 4 | 26 | 136 | 33 | 138 | 1.51e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1092750 | AT3G59470.3 | 37.838 | 111 | 58 | 3 | 26 | 134 | 69 | 170 | 3.37e-14 | 67.4 |
| Msa1092750 | AT3G59470.1 | 37.838 | 111 | 58 | 3 | 26 | 134 | 69 | 170 | 3.37e-14 | 67.4 |
| Msa1092750 | AT3G59470.2 | 37.838 | 111 | 58 | 3 | 26 | 134 | 81 | 182 | 3.86e-14 | 67.4 |
| Msa1092750 | AT3G07500.1 | 31.579 | 133 | 73 | 4 | 13 | 134 | 9 | 134 | 2.80e-13 | 64.7 |
Find 17 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTACCATCCTTCTTATT+TGG | 0.212526 | 7_3:+10390255 | None:intergenic |
| TTTAATTCATCATCACAATT+TGG | 0.253101 | 7_3:+10390441 | None:intergenic |
| ACTTCGAGAGACCGTTTCTA+AGG | 0.392704 | 7_3:-10390093 | None:intergenic |
| TCAAATACTTGTCCAATCAT+AGG | 0.403178 | 7_3:+10390417 | None:intergenic |
| GATGAATTAAAGCCTATGAT+TGG | 0.477938 | 7_3:-10390429 | Msa1092750:CDS |
| GGGTTATTTGCCAAATAAGA+AGG | 0.490320 | 7_3:-10390265 | Msa1092750:CDS |
| CTGTCAAGGTTAGAAGAAGA+AGG | 0.491761 | 7_3:-10390218 | Msa1092750:CDS |
| GCCAAATAAGAAGGATGGTA+AGG | 0.493986 | 7_3:-10390256 | Msa1092750:CDS |
| TTCCTTGTAGACCTTAGAAA+CGG | 0.517176 | 7_3:+10390082 | None:intergenic |
| TCGAAGTAAGTGGATGTGTA+TGG | 0.522532 | 7_3:+10390109 | None:intergenic |
| AGTTGATGTATCTTTGTCCA+TGG | 0.526717 | 7_3:+10390936 | None:intergenic |
| AAACGGTCTCTCGAAGTAAG+TGG | 0.540000 | 7_3:+10390099 | None:intergenic |
| AGAAGGGCTTTAACAAGAGA+AGG | 0.550276 | 7_3:-10390201 | Msa1092750:CDS |
| TATTTGCCAAATAAGAAGGA+TGG | 0.592394 | 7_3:-10390261 | Msa1092750:CDS |
| GCAAAGTGAATCTACTGTCA+AGG | 0.595204 | 7_3:-10390232 | Msa1092750:CDS |
| TGTCAAGGTTAGAAGAAGAA+GGG | 0.596214 | 7_3:-10390217 | Msa1092750:CDS |
| GTATTTGATACGTTAGCAGA+AGG | 0.613843 | 7_3:-10390402 | Msa1092750:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GGAAAACATAATAAATAAAA+AGG | + | chr7_3:10390511-10390530 | None:intergenic | 15.0% |
| !! | TCTTCAATAAAATAATAGAA+AGG | + | chr7_3:10390532-10390551 | None:intergenic | 15.0% |
| !! | GGAAAACATAATAAATAAAA+AGG | + | chr7_3:10390511-10390530 | None:intergenic | 15.0% |
| !! | TCTTCAATAAAATAATAGAA+AGG | + | chr7_3:10390532-10390551 | None:intergenic | 15.0% |
| !! | AACTAATTATAATATACCTG+AGG | + | chr7_3:10390131-10390150 | None:intergenic | 20.0% |
| !! | CAGGTATATTATAATTAGTT+TGG | - | chr7_3:10390131-10390150 | Msa1092750:CDS | 20.0% |
| !!! | TTTTGTTCCTATTTTTTTGT+TGG | - | chr7_3:10390218-10390237 | Msa1092750:CDS | 20.0% |
| !! | CCAAACTAAGAAAAAAAAAT+AGG | + | chr7_3:10390347-10390366 | None:intergenic | 20.0% |
| !!! | CCTATTTTTTTTTCTTAGTT+TGG | - | chr7_3:10390344-10390363 | Msa1092750:CDS | 20.0% |
| !! | TTTAATTCATCATCACAATT+TGG | + | chr7_3:10390591-10390610 | None:intergenic | 20.0% |
| !! | AACTAATTATAATATACCTG+AGG | + | chr7_3:10390131-10390150 | None:intergenic | 20.0% |
| !! | CAGGTATATTATAATTAGTT+TGG | - | chr7_3:10390131-10390150 | Msa1092750:CDS | 20.0% |
| !!! | TTTTGTTCCTATTTTTTTGT+TGG | - | chr7_3:10390218-10390237 | Msa1092750:CDS | 20.0% |
| !! | CCAAACTAAGAAAAAAAAAT+AGG | + | chr7_3:10390347-10390366 | None:intergenic | 20.0% |
| !!! | CCTATTTTTTTTTCTTAGTT+TGG | - | chr7_3:10390344-10390363 | Msa1092750:CDS | 20.0% |
| !! | TTTAATTCATCATCACAATT+TGG | + | chr7_3:10390591-10390610 | None:intergenic | 20.0% |
| !!! | ATTATAATTAGTTTGGCTTG+AGG | - | chr7_3:10390138-10390157 | Msa1092750:CDS | 25.0% |
| ! | ACAAAAAAAACCTATCTCTT+AGG | + | chr7_3:10390204-10390223 | None:intergenic | 25.0% |
| !! | TCATCGACTATAGATAAAAA+CGG | - | chr7_3:10390705-10390724 | Msa1092750:intron | 25.0% |
| !!! | ATGCAAATGTTGTTTTCAAA+TGG | - | chr7_3:10390856-10390875 | Msa1092750:intron | 25.0% |
| !!! | TGCAAATGTTGTTTTCAAAT+GGG | - | chr7_3:10390857-10390876 | Msa1092750:intron | 25.0% |
| !!! | ATTATAATTAGTTTGGCTTG+AGG | - | chr7_3:10390138-10390157 | Msa1092750:CDS | 25.0% |
| ! | ACAAAAAAAACCTATCTCTT+AGG | + | chr7_3:10390204-10390223 | None:intergenic | 25.0% |
| !! | TCATCGACTATAGATAAAAA+CGG | - | chr7_3:10390705-10390724 | Msa1092750:intron | 25.0% |
| !!! | ATGCAAATGTTGTTTTCAAA+TGG | - | chr7_3:10390856-10390875 | Msa1092750:intron | 25.0% |
| !!! | TGCAAATGTTGTTTTCAAAT+GGG | - | chr7_3:10390857-10390876 | Msa1092750:intron | 25.0% |
| !! | AACTTGTTTTCAAATTCCTC+AGG | - | chr7_3:10390112-10390131 | Msa1092750:CDS | 30.0% |
| !!! | TTGCTTTTTTCCTAAGAGAT+AGG | - | chr7_3:10390191-10390210 | Msa1092750:CDS | 30.0% |
| AGTGGAACCAACAAAAAAAT+AGG | + | chr7_3:10390228-10390247 | None:intergenic | 30.0% | |
| GATGAATTAAAGCCTATGAT+TGG | - | chr7_3:10390600-10390619 | Msa1092750:intron | 30.0% | |
| TCAAATACTTGTCCAATCAT+AGG | + | chr7_3:10390615-10390634 | None:intergenic | 30.0% | |
| !! | TATTTTGTTTGCTCGAAAGA+GGG | - | chr7_3:10390744-10390763 | Msa1092750:intron | 30.0% |
| TATTTGCCAAATAAGAAGGA+TGG | - | chr7_3:10390768-10390787 | Msa1092750:intron | 30.0% | |
| !!! | AAATGTTGTTTTCAAATGGG+TGG | - | chr7_3:10390860-10390879 | Msa1092750:intron | 30.0% |
| !!! | AATGTTGTTTTCAAATGGGT+GGG | - | chr7_3:10390861-10390880 | Msa1092750:intron | 30.0% |
| !! | AACTTGTTTTCAAATTCCTC+AGG | - | chr7_3:10390112-10390131 | Msa1092750:CDS | 30.0% |
| !!! | TTGCTTTTTTCCTAAGAGAT+AGG | - | chr7_3:10390191-10390210 | Msa1092750:CDS | 30.0% |
| AGTGGAACCAACAAAAAAAT+AGG | + | chr7_3:10390228-10390247 | None:intergenic | 30.0% | |
| GATGAATTAAAGCCTATGAT+TGG | - | chr7_3:10390600-10390619 | Msa1092750:intron | 30.0% | |
| TCAAATACTTGTCCAATCAT+AGG | + | chr7_3:10390615-10390634 | None:intergenic | 30.0% | |
| !! | TATTTTGTTTGCTCGAAAGA+GGG | - | chr7_3:10390744-10390763 | Msa1092750:intron | 30.0% |
| TATTTGCCAAATAAGAAGGA+TGG | - | chr7_3:10390768-10390787 | Msa1092750:intron | 30.0% | |
| !!! | AAATGTTGTTTTCAAATGGG+TGG | - | chr7_3:10390860-10390879 | Msa1092750:intron | 30.0% |
| !!! | AATGTTGTTTTCAAATGGGT+GGG | - | chr7_3:10390861-10390880 | Msa1092750:intron | 30.0% |
| AACCACCTAAGATTAATGCA+GGG | + | chr7_3:10390405-10390424 | None:intergenic | 35.0% | |
| AAACCACCTAAGATTAATGC+AGG | + | chr7_3:10390406-10390425 | None:intergenic | 35.0% | |
| GTATTTGATACGTTAGCAGA+AGG | - | chr7_3:10390627-10390646 | Msa1092750:intron | 35.0% | |
| !! | GTATTTTGTTTGCTCGAAAG+AGG | - | chr7_3:10390743-10390762 | Msa1092750:intron | 35.0% |
| GGGTTATTTGCCAAATAAGA+AGG | - | chr7_3:10390764-10390783 | Msa1092750:intron | 35.0% | |
| TCCTTACCATCCTTCTTATT+TGG | + | chr7_3:10390777-10390796 | None:intergenic | 35.0% | |
| TGTCAAGGTTAGAAGAAGAA+GGG | - | chr7_3:10390812-10390831 | Msa1092750:intron | 35.0% | |
| !!! | ATGTTGTTTTCAAATGGGTG+GGG | - | chr7_3:10390862-10390881 | Msa1092750:intron | 35.0% |
| AACCACCTAAGATTAATGCA+GGG | + | chr7_3:10390405-10390424 | None:intergenic | 35.0% | |
| AAACCACCTAAGATTAATGC+AGG | + | chr7_3:10390406-10390425 | None:intergenic | 35.0% | |
| GTATTTGATACGTTAGCAGA+AGG | - | chr7_3:10390627-10390646 | Msa1092750:intron | 35.0% | |
| !! | GTATTTTGTTTGCTCGAAAG+AGG | - | chr7_3:10390743-10390762 | Msa1092750:intron | 35.0% |
| GGGTTATTTGCCAAATAAGA+AGG | - | chr7_3:10390764-10390783 | Msa1092750:intron | 35.0% | |
| TCCTTACCATCCTTCTTATT+TGG | + | chr7_3:10390777-10390796 | None:intergenic | 35.0% | |
| TGTCAAGGTTAGAAGAAGAA+GGG | - | chr7_3:10390812-10390831 | Msa1092750:intron | 35.0% | |
| !!! | ATGTTGTTTTCAAATGGGTG+GGG | - | chr7_3:10390862-10390881 | Msa1092750:intron | 35.0% |
| CAACAACCAAATAGAACCAC+GGG | + | chr7_3:10390256-10390275 | None:intergenic | 40.0% | |
| CCAACAACCAAATAGAACCA+CGG | + | chr7_3:10390257-10390276 | None:intergenic | 40.0% | |
| AGAAGCCCTGCATTAATCTT+AGG | - | chr7_3:10390397-10390416 | Msa1092750:CDS | 40.0% | |
| GAAAAATACGCACTCTCTGT+CGG | - | chr7_3:10390663-10390682 | Msa1092750:intron | 40.0% | |
| ATAAAAACGGTGTGAAGCGT+TGG | - | chr7_3:10390718-10390737 | Msa1092750:intron | 40.0% | |
| GCCAAATAAGAAGGATGGTA+AGG | - | chr7_3:10390773-10390792 | Msa1092750:intron | 40.0% | |
| GCAAAGTGAATCTACTGTCA+AGG | - | chr7_3:10390797-10390816 | Msa1092750:intron | 40.0% | |
| CTGTCAAGGTTAGAAGAAGA+AGG | - | chr7_3:10390811-10390830 | Msa1092750:intron | 40.0% | |
| ! | AGAAGGGCTTTAACAAGAGA+AGG | - | chr7_3:10390828-10390847 | Msa1092750:intron | 40.0% |
| !!! | TGTTGTTTTCAAATGGGTGG+GGG | - | chr7_3:10390863-10390882 | Msa1092750:intron | 40.0% |
| TCGAAGTAAGTGGATGTGTA+TGG | + | chr7_3:10390923-10390942 | None:intergenic | 40.0% | |
| CAACAACCAAATAGAACCAC+GGG | + | chr7_3:10390256-10390275 | None:intergenic | 40.0% | |
| CCAACAACCAAATAGAACCA+CGG | + | chr7_3:10390257-10390276 | None:intergenic | 40.0% | |
| AGAAGCCCTGCATTAATCTT+AGG | - | chr7_3:10390397-10390416 | Msa1092750:CDS | 40.0% | |
| GAAAAATACGCACTCTCTGT+CGG | - | chr7_3:10390663-10390682 | Msa1092750:intron | 40.0% | |
| ATAAAAACGGTGTGAAGCGT+TGG | - | chr7_3:10390718-10390737 | Msa1092750:intron | 40.0% | |
| GCCAAATAAGAAGGATGGTA+AGG | - | chr7_3:10390773-10390792 | Msa1092750:intron | 40.0% | |
| GCAAAGTGAATCTACTGTCA+AGG | - | chr7_3:10390797-10390816 | Msa1092750:intron | 40.0% | |
| CTGTCAAGGTTAGAAGAAGA+AGG | - | chr7_3:10390811-10390830 | Msa1092750:intron | 40.0% | |
| ! | AGAAGGGCTTTAACAAGAGA+AGG | - | chr7_3:10390828-10390847 | Msa1092750:intron | 40.0% |
| !!! | TGTTGTTTTCAAATGGGTGG+GGG | - | chr7_3:10390863-10390882 | Msa1092750:intron | 40.0% |
| TCGAAGTAAGTGGATGTGTA+TGG | + | chr7_3:10390923-10390942 | None:intergenic | 40.0% | |
| ! | CCGTGGTTCTATTTGGTTGT+TGG | - | chr7_3:10390254-10390273 | Msa1092750:CDS | 45.0% |
| AGCCCTGCATTAATCTTAGG+TGG | - | chr7_3:10390400-10390419 | Msa1092750:CDS | 45.0% | |
| AAACGGTCTCTCGAAGTAAG+TGG | + | chr7_3:10390933-10390952 | None:intergenic | 45.0% | |
| ! | CCGTGGTTCTATTTGGTTGT+TGG | - | chr7_3:10390254-10390273 | Msa1092750:CDS | 45.0% |
| AGCCCTGCATTAATCTTAGG+TGG | - | chr7_3:10390400-10390419 | Msa1092750:CDS | 45.0% | |
| AAACGGTCTCTCGAAGTAAG+TGG | + | chr7_3:10390933-10390952 | None:intergenic | 45.0% | |
| !!! | ATTTTTATTTTGTTAATTTT+AGG | - | chr7_3:10390551-10390570 | Msa1092750:intron | 5.0% |
| !!! | ATTTTTATTTTGTTAATTTT+AGG | - | chr7_3:10390551-10390570 | Msa1092750:intron | 5.0% |
| ! | TGCTGTCCCGTGGTTCTATT+TGG | - | chr7_3:10390247-10390266 | Msa1092750:CDS | 50.0% |
| ! | GTTTTCAAATGGGTGGGGGA+AGG | - | chr7_3:10390867-10390886 | Msa1092750:intron | 50.0% |
| ! | TGCTGTCCCGTGGTTCTATT+TGG | - | chr7_3:10390247-10390266 | Msa1092750:CDS | 50.0% |
| ! | GTTTTCAAATGGGTGGGGGA+AGG | - | chr7_3:10390867-10390886 | Msa1092750:intron | 50.0% |
| ATAGAACCACGGGACAGCAG+TGG | + | chr7_3:10390246-10390265 | None:intergenic | 55.0% | |
| ATAGAACCACGGGACAGCAG+TGG | + | chr7_3:10390246-10390265 | None:intergenic | 55.0% | |
| TTGGTTCCACTGCTGTCCCG+TGG | - | chr7_3:10390237-10390256 | Msa1092750:CDS | 60.0% | |
| TTGGTTCCACTGCTGTCCCG+TGG | - | chr7_3:10390237-10390256 | Msa1092750:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 10390095 | 10390956 | 10390095 | ID=Msa1092750;Name=Msa1092750 |
| chr7_3 | mRNA | 10390095 | 10390956 | 10390095 | ID=Msa1092750-mRNA-1;Parent=Msa1092750;Name=Msa1092750-mRNA-1;_AED=0.42;_eAED=0.44;_QI=0|0|0|0.5|1|1|2|0|140 |
| chr7_3 | exon | 10390918 | 10390956 | 10390918 | ID=Msa1092750-mRNA-1:exon:2770;Parent=Msa1092750-mRNA-1 |
| chr7_3 | exon | 10390095 | 10390478 | 10390095 | ID=Msa1092750-mRNA-1:exon:2769;Parent=Msa1092750-mRNA-1 |
| chr7_3 | CDS | 10390918 | 10390956 | 10390918 | ID=Msa1092750-mRNA-1:cds;Parent=Msa1092750-mRNA-1 |
| chr7_3 | CDS | 10390095 | 10390478 | 10390095 | ID=Msa1092750-mRNA-1:cds;Parent=Msa1092750-mRNA-1 |
| Gene Sequence |
| Protein sequence |
