Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1093010 | ABN08427.1 | 72.897 | 107 | 25 | 2 | 2 | 104 | 12 | 118 | 1.61e-43 | 148 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1093010 | A2Q477 | 72.897 | 107 | 25 | 2 | 2 | 104 | 12 | 118 | 7.68e-44 | 148 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1093010 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1093010 | MtrunA17_Chr4g0064011 | 71.698 | 106 | 27 | 2 | 2 | 104 | 127 | 232 | 3.27e-44 | 143 |
| Msa1093010 | MtrunA17_Chr3g0122071 | 60.000 | 105 | 36 | 4 | 2 | 101 | 119 | 222 | 8.61e-34 | 116 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1093010 | AT5G22990.1 | 46.341 | 82 | 41 | 2 | 2 | 80 | 238 | 319 | 2.91e-16 | 72.4 |
| Msa1093010 | AT2G18490.1 | 52.941 | 51 | 24 | 0 | 2 | 52 | 161 | 211 | 4.28e-15 | 68.6 |
| Msa1093010 | AT2G18490.2 | 52.941 | 51 | 24 | 0 | 2 | 52 | 211 | 261 | 6.41e-15 | 68.6 |
| Msa1093010 | AT2G15740.1 | 53.333 | 60 | 24 | 1 | 2 | 61 | 236 | 291 | 1.27e-14 | 68.2 |
| Msa1093010 | AT5G42640.1 | 52.941 | 51 | 24 | 0 | 2 | 52 | 235 | 285 | 2.14e-14 | 67.4 |
Find 17 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACATGACTAGCGAATTTC+TGG | 0.115058 | 7_3:-11998980 | None:intergenic |
| TCCGGAGATTTGGTCTCTTT+CGG | 0.299968 | 7_3:-11999061 | None:intergenic |
| AAGCGGAGTAAAAGTTCAAT+TGG | 0.407015 | 7_3:+11999199 | Msa1093010:CDS |
| TTACTCCGCTTATAGGAGTA+GGG | 0.455217 | 7_3:-11999187 | None:intergenic |
| TAATCAACAGAAGCAACAAC+TGG | 0.464101 | 7_3:-11999119 | None:intergenic |
| GAAATTACTTTGTTGCACTT+TGG | 0.483320 | 7_3:-11998951 | None:intergenic |
| TTTACTCCGCTTATAGGAGT+AGG | 0.485433 | 7_3:-11999188 | None:intergenic |
| ATGTTTATTGTGTGGAAACA+TGG | 0.497240 | 7_3:-11998913 | None:intergenic |
| CACATGACTAGCGAATTTCT+GGG | 0.497762 | 7_3:-11998979 | None:intergenic |
| AAGTCTGAAACTCAAGAAGA+AGG | 0.538254 | 7_3:+11999014 | Msa1093010:CDS |
| TCCGAAAGAGACCAAATCTC+CGG | 0.539140 | 7_3:+11999060 | Msa1093010:CDS |
| TACTCCGCTTATAGGAGTAG+GGG | 0.584715 | 7_3:-11999186 | None:intergenic |
| AGCGCCCCTACTCCTATAAG+CGG | 0.584814 | 7_3:+11999182 | Msa1093010:CDS |
| TGCGGTCCATGTTTATTGTG+TGG | 0.611060 | 7_3:-11998921 | None:intergenic |
| CCAGAAATTCGCTAGTCATG+TGG | 0.623044 | 7_3:+11998980 | Msa1093010:CDS |
| ATGTTTCCACACAATAAACA+TGG | 0.631696 | 7_3:+11998915 | Msa1093010:CDS |
| TTGCACTTTGGACATGTGTG+CGG | 0.654587 | 7_3:-11998939 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGTTTCCACACAATAAACA+TGG | + | chr7_3:11998915-11998934 | Msa1093010:CDS | 30.0% | |
| GAAATTACTTTGTTGCACTT+TGG | - | chr7_3:11998954-11998973 | None:intergenic | 30.0% | |
| GAAAAAAGAGATACATGTCT+AGG | + | chr7_3:11999036-11999055 | Msa1093010:CDS | 30.0% | |
| ! | AAGTCTGAAACTCAAGAAGA+AGG | + | chr7_3:11999014-11999033 | Msa1093010:CDS | 35.0% |
| ! | GTTCATTGAGCTTTTGAATC+CGG | - | chr7_3:11999082-11999101 | None:intergenic | 35.0% |
| TAATCAACAGAAGCAACAAC+TGG | - | chr7_3:11999122-11999141 | None:intergenic | 35.0% | |
| ! | TGAACTTTTACTCCGCTTAT+AGG | - | chr7_3:11999197-11999216 | None:intergenic | 35.0% |
| AAGCGGAGTAAAAGTTCAAT+TGG | + | chr7_3:11999199-11999218 | Msa1093010:CDS | 35.0% | |
| CACATGACTAGCGAATTTCT+GGG | - | chr7_3:11998982-11999001 | None:intergenic | 40.0% | |
| ! | AGCTTTTGAATCCGGAGATT+TGG | - | chr7_3:11999074-11999093 | None:intergenic | 40.0% |
| TTACTCCGCTTATAGGAGTA+GGG | - | chr7_3:11999190-11999209 | None:intergenic | 40.0% | |
| TTTACTCCGCTTATAGGAGT+AGG | - | chr7_3:11999191-11999210 | None:intergenic | 40.0% | |
| TGCGGTCCATGTTTATTGTG+TGG | - | chr7_3:11998924-11998943 | None:intergenic | 45.0% | |
| TTGCACTTTGGACATGTGTG+CGG | - | chr7_3:11998942-11998961 | None:intergenic | 45.0% | |
| CCACATGACTAGCGAATTTC+TGG | - | chr7_3:11998983-11999002 | None:intergenic | 45.0% | |
| CCAGAAATTCGCTAGTCATG+TGG | + | chr7_3:11998980-11998999 | Msa1093010:CDS | 45.0% | |
| TCCGAAAGAGACCAAATCTC+CGG | + | chr7_3:11999060-11999079 | Msa1093010:CDS | 45.0% | |
| ! | TCCGGAGATTTGGTCTCTTT+CGG | - | chr7_3:11999064-11999083 | None:intergenic | 45.0% |
| TACTCCGCTTATAGGAGTAG+GGG | - | chr7_3:11999189-11999208 | None:intergenic | 45.0% | |
| AGCGCCCCTACTCCTATAAG+CGG | + | chr7_3:11999182-11999201 | Msa1093010:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 11998915 | 11999238 | 11998915 | ID=Msa1093010;Name=Msa1093010 |
| chr7_3 | mRNA | 11998915 | 11999238 | 11998915 | ID=Msa1093010-mRNA-1;Parent=Msa1093010;Name=Msa1093010-mRNA-1;_AED=0.48;_eAED=0.49;_QI=0|0|0|1|0|0|2|0|104 |
| chr7_3 | exon | 11998915 | 11999133 | 11998915 | ID=Msa1093010-mRNA-1:exon:2889;Parent=Msa1093010-mRNA-1 |
| chr7_3 | exon | 11999143 | 11999238 | 11999143 | ID=Msa1093010-mRNA-1:exon:2890;Parent=Msa1093010-mRNA-1 |
| chr7_3 | CDS | 11998915 | 11999133 | 11998915 | ID=Msa1093010-mRNA-1:cds;Parent=Msa1093010-mRNA-1 |
| chr7_3 | CDS | 11999143 | 11999238 | 11999143 | ID=Msa1093010-mRNA-1:cds;Parent=Msa1093010-mRNA-1 |
| Gene Sequence |
| Protein sequence |
