AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1094500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1094500 MtrunA17_Chr7g0224031 83.495 103 17 0 221 323 49 151 2.26e-54 177
Msa1094500 MtrunA17_Chr7g0224051 73.016 126 29 1 203 323 680 805 1.22e-52 186
Msa1094500 MtrunA17_Chr7g0224051 46.748 246 65 6 1 186 314 553 3.97e-50 179
Msa1094500 MtrunA17_Chr3g0114161 75.962 104 24 1 221 323 833 936 3.03e-45 165
Msa1094500 MtrunA17_Chr3g0114161 66.957 115 32 2 1 109 427 541 3.29e-36 138
Msa1094500 MtrunA17_Chr3g0114161 54.545 77 33 2 110 186 606 680 1.56e-13 71.6
Msa1094500 MtrunA17_Chr5g0437621 46.939 196 71 5 158 323 802 994 7.52e-44 160
Msa1094500 MtrunA17_Chr1g0191141 73.786 103 26 1 221 323 690 791 6.66e-43 158
Msa1094500 MtrunA17_Chr1g0191141 38.017 242 86 7 1 186 307 540 3.69e-32 127
Msa1094500 MtrunA17_Chr7g0233021 48.571 70 36 0 218 287 833 902 5.41e-14 73.2
Msa1094500 MtrunA17_Chr4g0047081 45.833 72 39 0 218 289 835 906 8.94e-14 72.4
Msa1094500 MtrunA17_Chr7g0232991 50.000 66 33 0 218 283 264 329 9.56e-14 72.0
Msa1094500 MtrunA17_Chr7g0225791 40.964 83 49 0 218 300 885 967 2.71e-13 70.9
Msa1094500 MtrunA17_Chr7g0232971 49.275 69 35 0 219 287 897 965 2.95e-13 70.9
Msa1094500 MtrunA17_Chr7g0233031 49.231 65 33 0 223 287 858 922 1.11e-12 68.9
Msa1094500 MtrunA17_Chr7g0233001 44.444 72 40 0 218 289 120 191 1.98e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1094500 AT5G20420.1 46.667 180 69 5 169 323 771 948 2.24e-40 151
Msa1094500 AT3G42670.2 68.269 104 32 1 221 324 841 943 3.68e-39 147
Msa1094500 AT3G42670.3 68.269 104 32 1 221 324 841 943 3.68e-39 147
Msa1094500 AT3G42670.1 68.269 104 32 1 221 324 841 943 3.68e-39 147
Msa1094500 AT3G42670.4 68.269 104 32 1 221 324 843 945 3.90e-39 147
Msa1094500 AT3G24340.1 43.119 109 47 3 177 277 694 795 4.73e-14 73.6
Msa1094500 AT1G05490.1 48.571 70 36 0 218 287 1000 1069 1.32e-12 69.3

Find 47 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGCACTTACATGAAGAATT+TGG 0.177957 7_3:-16378530 Msa1094500:CDS
AGCACTTCGTGAACAAATTT+TGG 0.230747 7_3:+16376921 None:intergenic
AACCTTGATTTAGTGCTTCT+AGG 0.271299 7_3:+16377050 None:intergenic
TTGTCTTCTTCTTTAGATAA+TGG 0.275139 7_3:+16377651 None:intergenic
GATGCACACAAGGAAATAAT+TGG 0.325465 7_3:-16378550 Msa1094500:CDS
CTAAAGAAGAAGACAATGTT+TGG 0.328401 7_3:-16377644 Msa1094500:CDS
TTTGTTTCTTCATTAATAGT+TGG 0.352376 7_3:+16378484 None:intergenic
AAGGTTGAAGAAATGCTTAA+TGG 0.374531 7_3:-16377033 Msa1094500:CDS
GAAGAATTTGGATAGTCTTC+CGG 0.394022 7_3:-16378518 Msa1094500:CDS
AAGAAAAGAAATGTTGAGAA+AGG 0.409608 7_3:+16377459 None:intergenic
TTTCTTTCGCAAAACATTGT+TGG 0.412358 7_3:-16377565 Msa1094500:CDS
CATGAAGAACAAGATAATAA+TGG 0.418039 7_3:-16378592 Msa1094500:CDS
GAAATGAATCCCTTCCCATT+TGG 0.419989 7_3:+16378675 None:intergenic
TGAAGAAACAAACAAGTTTG+AGG 0.450964 7_3:-16378473 Msa1094500:CDS
GCAAAACATTGTTGGTTTCG+TGG 0.455573 7_3:-16377557 Msa1094500:CDS
TAAAGAAGAAGACAATGTTT+GGG 0.466098 7_3:-16377643 Msa1094500:CDS
TTGATGACTTGTCCGCCACT+TGG 0.468292 7_3:-16378411 Msa1094500:CDS
ATATCACATGCTCTTGGAGC+AGG 0.473334 7_3:-16377487 Msa1094500:CDS
GATACTGCTTTAATAGCAAA+AGG 0.477495 7_3:-16378379 Msa1094500:intron
AAATACTTGAGGAGAGGTAA+TGG 0.479844 7_3:-16376882 Msa1094500:CDS
TCGTCAAGTATCAAGAGTCC+AGG 0.499815 7_3:+16377083 None:intergenic
GCGGCGAAGGAGAATCTTCA+TGG 0.500291 7_3:-16376829 Msa1094500:CDS
TTTCAGATCTTTCTTACGTG+AGG 0.503386 7_3:+16377527 None:intergenic
TTCAGATCTTTCTTACGTGA+GGG 0.523183 7_3:+16377528 None:intergenic
TCTTTAGATAATGGTTCACC+AGG 0.523606 7_3:+16377660 None:intergenic
GAAAGATCTGAAACAAGTGT+TGG 0.526144 7_3:-16377517 Msa1094500:CDS
ATCCTAGAAGCACTAAATCA+AGG 0.536502 7_3:-16377052 Msa1094500:CDS
TGATGACTTGTCCGCCACTT+GGG 0.540770 7_3:-16378410 Msa1094500:CDS
TGTGTCATATCACATGCTCT+TGG 0.541798 7_3:-16377493 Msa1094500:CDS
GATCTTTCTTACGTGAGGGA+TGG 0.542290 7_3:+16377532 None:intergenic
ATAGTTGGCTCTTCTTCAGC+CGG 0.543638 7_3:+16378499 None:intergenic
ATTTGCTTGAGGCGCGGCGA+AGG 0.544026 7_3:-16376842 Msa1094500:CDS
TCTTCATGGAAAACATTGCT+AGG 0.547442 7_3:-16376815 Msa1094500:CDS
CACAACATTTGCTTGAGGCG+CGG 0.558042 7_3:-16376848 Msa1094500:CDS
ATCTATTTCTTCCCAAGTGG+CGG 0.558498 7_3:+16378399 None:intergenic
AGTTAGATTCTAAATACTTG+AGG 0.561826 7_3:-16376893 Msa1094500:CDS
AATGTCGAAAAGAGATGATG+AGG 0.575153 7_3:-16378443 Msa1094500:CDS
ACTCATTCTACTGATCCTCC+TGG 0.582157 7_3:-16377678 Msa1094500:CDS
AAAACATTCAGAGAAAGCCC+TGG 0.598405 7_3:-16377101 Msa1094500:intron
TTAGATAATGGTTCACCAGG+AGG 0.622558 7_3:+16377663 None:intergenic
GATTCTAAATACTTGAGGAG+AGG 0.628538 7_3:-16376888 Msa1094500:CDS
AAAATCAACTCACAATCATG+AGG 0.638562 7_3:-16376792 None:intergenic
GTTAAATGTTGATGCACACA+AGG 0.641852 7_3:-16378560 Msa1094500:CDS
AGTATCTATTTCTTCCCAAG+TGG 0.646380 7_3:+16378396 None:intergenic
AAAGAGGAGAAACAATGCAG+TGG 0.651340 7_3:-16377768 Msa1094500:intron
CGTCAAGTATCAAGAGTCCA+GGG 0.651876 7_3:+16377084 None:intergenic
GAAAGCACAACATTTGCTTG+AGG 0.655037 7_3:-16376853 Msa1094500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATGTATATTCTTGTTA+AGG + chr7_3:16377510-16377529 None:intergenic 15.0%
!!! TTTAAATTTAGTTCCTTATT+TGG - chr7_3:16377525-16377544 Msa1094500:CDS 15.0%
!! ATATAAATAATTTATCAGGT+AGG + chr7_3:16377663-16377682 None:intergenic 15.0%
!! TATTTATATATATAAGTAGG+TGG - chr7_3:16377674-16377693 Msa1094500:CDS 15.0%
!!! AGAAATTCAAAAACTTTTTT+CGG + chr7_3:16377902-16377921 None:intergenic 15.0%
!! TTAAATGTATATTCTTGTTA+AGG + chr7_3:16377510-16377529 None:intergenic 15.0%
!!! TTTAAATTTAGTTCCTTATT+TGG - chr7_3:16377525-16377544 Msa1094500:CDS 15.0%
!! ATATAAATAATTTATCAGGT+AGG + chr7_3:16377663-16377682 None:intergenic 15.0%
!! TATTTATATATATAAGTAGG+TGG - chr7_3:16377674-16377693 Msa1094500:CDS 15.0%
!!! AGAAATTCAAAAACTTTTTT+CGG + chr7_3:16377902-16377921 None:intergenic 15.0%
!!! TTTGTTTCTTCATTAATAGT+TGG + chr7_3:16377001-16377020 None:intergenic 20.0%
!!! ATTGATTCATATATATTGTC+AGG + chr7_3:16377267-16377286 None:intergenic 20.0%
!!! TGATTTTTTCTACTTTTCTA+TGG - chr7_3:16377298-16377317 Msa1094500:intron 20.0%
!!! GATTTTTTCTACTTTTCTAT+GGG - chr7_3:16377299-16377318 Msa1094500:intron 20.0%
!!! GAAATTCAAAAACTTTTTTC+GGG + chr7_3:16377901-16377920 None:intergenic 20.0%
!!! TGATCTCTTTAATTCTAAAA+AGG + chr7_3:16378325-16378344 None:intergenic 20.0%
!!! TTTGTTTCTTCATTAATAGT+TGG + chr7_3:16377001-16377020 None:intergenic 20.0%
!!! ATTGATTCATATATATTGTC+AGG + chr7_3:16377267-16377286 None:intergenic 20.0%
!!! TGATTTTTTCTACTTTTCTA+TGG - chr7_3:16377298-16377317 Msa1094500:intron 20.0%
!!! GATTTTTTCTACTTTTCTAT+GGG - chr7_3:16377299-16377318 Msa1094500:intron 20.0%
!!! GAAATTCAAAAACTTTTTTC+GGG + chr7_3:16377901-16377920 None:intergenic 20.0%
!!! TGATCTCTTTAATTCTAAAA+AGG + chr7_3:16378325-16378344 None:intergenic 20.0%
! CATGAAGAACAAGATAATAA+TGG - chr7_3:16376890-16376909 Msa1094500:CDS 25.0%
!!! GTTTAATTTTGATACGCTAT+CGG - chr7_3:16377190-16377209 Msa1094500:intron 25.0%
!! TATGATAGGTTGTAATTTGT+TGG + chr7_3:16377234-16377253 None:intergenic 25.0%
! CAGGTCATTAAAAATATGAT+AGG + chr7_3:16377248-16377267 None:intergenic 25.0%
! ACAATAGACATAACCAAATA+AGG + chr7_3:16377541-16377560 None:intergenic 25.0%
! GTTTAACAAATCGTTATTCT+TGG - chr7_3:16377599-16377618 Msa1094500:CDS 25.0%
! TTGTCTTCTTCTTTAGATAA+TGG + chr7_3:16377834-16377853 None:intergenic 25.0%
! TAAAGAAGAAGACAATGTTT+GGG - chr7_3:16377839-16377858 Msa1094500:intron 25.0%
! AAGAAAAGAAATGTTGAGAA+AGG + chr7_3:16378026-16378045 None:intergenic 25.0%
!!! AATTTTGGTGCAAGTATTAA+TGG + chr7_3:16378082-16378101 None:intergenic 25.0%
! CAAAGAGTTAAAAAAATGGA+AGG - chr7_3:16378120-16378139 Msa1094500:intron 25.0%
! AATATTCACAAAAATTGCTC+TGG + chr7_3:16378518-16378537 None:intergenic 25.0%
! AGTTAGATTCTAAATACTTG+AGG - chr7_3:16378589-16378608 Msa1094500:CDS 25.0%
! CATGAAGAACAAGATAATAA+TGG - chr7_3:16376890-16376909 Msa1094500:CDS 25.0%
!!! GTTTAATTTTGATACGCTAT+CGG - chr7_3:16377190-16377209 Msa1094500:intron 25.0%
!! TATGATAGGTTGTAATTTGT+TGG + chr7_3:16377234-16377253 None:intergenic 25.0%
! CAGGTCATTAAAAATATGAT+AGG + chr7_3:16377248-16377267 None:intergenic 25.0%
! ACAATAGACATAACCAAATA+AGG + chr7_3:16377541-16377560 None:intergenic 25.0%
! GTTTAACAAATCGTTATTCT+TGG - chr7_3:16377599-16377618 Msa1094500:CDS 25.0%
! TTGTCTTCTTCTTTAGATAA+TGG + chr7_3:16377834-16377853 None:intergenic 25.0%
! TAAAGAAGAAGACAATGTTT+GGG - chr7_3:16377839-16377858 Msa1094500:intron 25.0%
! AAGAAAAGAAATGTTGAGAA+AGG + chr7_3:16378026-16378045 None:intergenic 25.0%
!!! AATTTTGGTGCAAGTATTAA+TGG + chr7_3:16378082-16378101 None:intergenic 25.0%
! CAAAGAGTTAAAAAAATGGA+AGG - chr7_3:16378120-16378139 Msa1094500:intron 25.0%
! AATATTCACAAAAATTGCTC+TGG + chr7_3:16378518-16378537 None:intergenic 25.0%
! AGTTAGATTCTAAATACTTG+AGG - chr7_3:16378589-16378608 Msa1094500:CDS 25.0%
!!! ATTTTGAAACCAAATGGGAA+GGG - chr7_3:16376798-16376817 Msa1094500:CDS 30.0%
GAAGGGATTCATTTCAAAAA+AGG - chr7_3:16376815-16376834 Msa1094500:CDS 30.0%
TGAAGAAACAAACAAGTTTG+AGG - chr7_3:16377009-16377028 Msa1094500:CDS 30.0%
GATACTGCTTTAATAGCAAA+AGG - chr7_3:16377103-16377122 Msa1094500:intron 30.0%
ACGGTATATAATACTTGAAC+TGG + chr7_3:16377336-16377355 None:intergenic 30.0%
AAGTATTATATACCGTTGCA+GGG - chr7_3:16377340-16377359 Msa1094500:intron 30.0%
CTACTGCAATTTGTATATAG+TGG - chr7_3:16377561-16377580 Msa1094500:CDS 30.0%
CTAAAGAAGAAGACAATGTT+TGG - chr7_3:16377838-16377857 Msa1094500:intron 30.0%
! ATGCATCTTTTCTTTGAATC+AGG + chr7_3:16377873-16377892 None:intergenic 30.0%
TTTCTTTCGCAAAACATTGT+TGG - chr7_3:16377917-16377936 Msa1094500:intron 30.0%
!!! AGTTACGTGAAGTTTTTTCT+AGG - chr7_3:16378049-16378068 Msa1094500:intron 30.0%
! TATGCGTAGAGTGTAAATTT+TGG + chr7_3:16378097-16378116 None:intergenic 30.0%
AGCGCAAAGAGTTAAAAAAA+TGG - chr7_3:16378116-16378135 Msa1094500:intron 30.0%
! ACATGTTTTACATCACCAAT+AGG + chr7_3:16378235-16378254 None:intergenic 30.0%
!! TGGTGATGTAAAACATGTTT+TGG - chr7_3:16378237-16378256 Msa1094500:intron 30.0%
!! AAACATGTTTTGGATTGTCT+TGG - chr7_3:16378247-16378266 Msa1094500:intron 30.0%
TGTCTTGGCAAAAAATAAAG+TGG - chr7_3:16378262-16378281 Msa1094500:intron 30.0%
!!! ATGTTTTAGCCATGTAGTTT+TGG + chr7_3:16378368-16378387 None:intergenic 30.0%
AAGGTTGAAGAAATGCTTAA+TGG - chr7_3:16378449-16378468 Msa1094500:CDS 30.0%
!!! ATTTTGAAACCAAATGGGAA+GGG - chr7_3:16376798-16376817 Msa1094500:CDS 30.0%
GAAGGGATTCATTTCAAAAA+AGG - chr7_3:16376815-16376834 Msa1094500:CDS 30.0%
TGAAGAAACAAACAAGTTTG+AGG - chr7_3:16377009-16377028 Msa1094500:CDS 30.0%
GATACTGCTTTAATAGCAAA+AGG - chr7_3:16377103-16377122 Msa1094500:intron 30.0%
ACGGTATATAATACTTGAAC+TGG + chr7_3:16377336-16377355 None:intergenic 30.0%
AAGTATTATATACCGTTGCA+GGG - chr7_3:16377340-16377359 Msa1094500:intron 30.0%
CTACTGCAATTTGTATATAG+TGG - chr7_3:16377561-16377580 Msa1094500:CDS 30.0%
CTAAAGAAGAAGACAATGTT+TGG - chr7_3:16377838-16377857 Msa1094500:intron 30.0%
! ATGCATCTTTTCTTTGAATC+AGG + chr7_3:16377873-16377892 None:intergenic 30.0%
TTTCTTTCGCAAAACATTGT+TGG - chr7_3:16377917-16377936 Msa1094500:intron 30.0%
!!! AGTTACGTGAAGTTTTTTCT+AGG - chr7_3:16378049-16378068 Msa1094500:intron 30.0%
! TATGCGTAGAGTGTAAATTT+TGG + chr7_3:16378097-16378116 None:intergenic 30.0%
AGCGCAAAGAGTTAAAAAAA+TGG - chr7_3:16378116-16378135 Msa1094500:intron 30.0%
! ACATGTTTTACATCACCAAT+AGG + chr7_3:16378235-16378254 None:intergenic 30.0%
!! TGGTGATGTAAAACATGTTT+TGG - chr7_3:16378237-16378256 Msa1094500:intron 30.0%
!! AAACATGTTTTGGATTGTCT+TGG - chr7_3:16378247-16378266 Msa1094500:intron 30.0%
TGTCTTGGCAAAAAATAAAG+TGG - chr7_3:16378262-16378281 Msa1094500:intron 30.0%
!!! ATGTTTTAGCCATGTAGTTT+TGG + chr7_3:16378368-16378387 None:intergenic 30.0%
AAGGTTGAAGAAATGCTTAA+TGG - chr7_3:16378449-16378468 Msa1094500:CDS 30.0%
!!! GATTTTGAAACCAAATGGGA+AGG - chr7_3:16376797-16376816 Msa1094500:CDS 35.0%
GTTAAATGTTGATGCACACA+AGG - chr7_3:16376922-16376941 Msa1094500:CDS 35.0%
GATGCACACAAGGAAATAAT+TGG - chr7_3:16376932-16376951 Msa1094500:CDS 35.0%
TGGCACTTACATGAAGAATT+TGG - chr7_3:16376952-16376971 Msa1094500:CDS 35.0%
GAAGAATTTGGATAGTCTTC+CGG - chr7_3:16376964-16376983 Msa1094500:CDS 35.0%
AATGTCGAAAAGAGATGATG+AGG - chr7_3:16377039-16377058 Msa1094500:CDS 35.0%
! AGTATCTATTTCTTCCCAAG+TGG + chr7_3:16377089-16377108 None:intergenic 35.0%
ATAGCAAAAGGTCTTCTTGA+TGG - chr7_3:16377115-16377134 Msa1094500:intron 35.0%
!! GATAGGTTGTAATTTGTTGG+TGG + chr7_3:16377231-16377250 None:intergenic 35.0%
CAAGTATTATATACCGTTGC+AGG - chr7_3:16377339-16377358 Msa1094500:intron 35.0%
!! AAAGGTTTTAGCAGATACCA+AGG - chr7_3:16377372-16377391 Msa1094500:intron 35.0%
CTCATGTTCACAAATTTCCT+TGG + chr7_3:16377392-16377411 None:intergenic 35.0%
TGAATATTCTGTCGTAGTTG+CGG - chr7_3:16377427-16377446 Msa1094500:intron 35.0%
AGTAGGTGGAAAGACTAAAA+TGG - chr7_3:16377688-16377707 Msa1094500:CDS 35.0%
AAGACTAAAATGGCACAAAG+AGG - chr7_3:16377698-16377717 Msa1094500:CDS 35.0%
! TCTTTAGATAATGGTTCACC+AGG + chr7_3:16377825-16377844 None:intergenic 35.0%
TTCAGATCTTTCTTACGTGA+GGG + chr7_3:16377957-16377976 None:intergenic 35.0%
TTTCAGATCTTTCTTACGTG+AGG + chr7_3:16377958-16377977 None:intergenic 35.0%
! GAAAGATCTGAAACAAGTGT+TGG - chr7_3:16377965-16377984 Msa1094500:intron 35.0%
! TCAAATCTGAAGTGGTTCAA+CGG - chr7_3:16378174-16378193 Msa1094500:intron 35.0%
TTTACATCACCAATAGGTCT+TGG + chr7_3:16378229-16378248 None:intergenic 35.0%
ATTTGCACACCAAAACTACA+TGG - chr7_3:16378356-16378375 Msa1094500:intron 35.0%
!! ATCCTAGAAGCACTAAATCA+AGG - chr7_3:16378430-16378449 Msa1094500:CDS 35.0%
AACCTTGATTTAGTGCTTCT+AGG + chr7_3:16378435-16378454 None:intergenic 35.0%
! AGCACTTCGTGAACAAATTT+TGG + chr7_3:16378564-16378583 None:intergenic 35.0%
GATTCTAAATACTTGAGGAG+AGG - chr7_3:16378594-16378613 Msa1094500:CDS 35.0%
AAATACTTGAGGAGAGGTAA+TGG - chr7_3:16378600-16378619 Msa1094500:CDS 35.0%
TCTTCATGGAAAACATTGCT+AGG - chr7_3:16378667-16378686 Msa1094500:CDS 35.0%
!!! GATTTTGAAACCAAATGGGA+AGG - chr7_3:16376797-16376816 Msa1094500:CDS 35.0%
GTTAAATGTTGATGCACACA+AGG - chr7_3:16376922-16376941 Msa1094500:CDS 35.0%
GATGCACACAAGGAAATAAT+TGG - chr7_3:16376932-16376951 Msa1094500:CDS 35.0%
TGGCACTTACATGAAGAATT+TGG - chr7_3:16376952-16376971 Msa1094500:CDS 35.0%
GAAGAATTTGGATAGTCTTC+CGG - chr7_3:16376964-16376983 Msa1094500:CDS 35.0%
AATGTCGAAAAGAGATGATG+AGG - chr7_3:16377039-16377058 Msa1094500:CDS 35.0%
! AGTATCTATTTCTTCCCAAG+TGG + chr7_3:16377089-16377108 None:intergenic 35.0%
ATAGCAAAAGGTCTTCTTGA+TGG - chr7_3:16377115-16377134 Msa1094500:intron 35.0%
!! GATAGGTTGTAATTTGTTGG+TGG + chr7_3:16377231-16377250 None:intergenic 35.0%
CAAGTATTATATACCGTTGC+AGG - chr7_3:16377339-16377358 Msa1094500:intron 35.0%
!! AAAGGTTTTAGCAGATACCA+AGG - chr7_3:16377372-16377391 Msa1094500:intron 35.0%
CTCATGTTCACAAATTTCCT+TGG + chr7_3:16377392-16377411 None:intergenic 35.0%
TGAATATTCTGTCGTAGTTG+CGG - chr7_3:16377427-16377446 Msa1094500:intron 35.0%
AGTAGGTGGAAAGACTAAAA+TGG - chr7_3:16377688-16377707 Msa1094500:CDS 35.0%
AAGACTAAAATGGCACAAAG+AGG - chr7_3:16377698-16377717 Msa1094500:CDS 35.0%
! TCTTTAGATAATGGTTCACC+AGG + chr7_3:16377825-16377844 None:intergenic 35.0%
TTCAGATCTTTCTTACGTGA+GGG + chr7_3:16377957-16377976 None:intergenic 35.0%
TTTCAGATCTTTCTTACGTG+AGG + chr7_3:16377958-16377977 None:intergenic 35.0%
! GAAAGATCTGAAACAAGTGT+TGG - chr7_3:16377965-16377984 Msa1094500:intron 35.0%
! TCAAATCTGAAGTGGTTCAA+CGG - chr7_3:16378174-16378193 Msa1094500:intron 35.0%
TTTACATCACCAATAGGTCT+TGG + chr7_3:16378229-16378248 None:intergenic 35.0%
ATTTGCACACCAAAACTACA+TGG - chr7_3:16378356-16378375 Msa1094500:intron 35.0%
!! ATCCTAGAAGCACTAAATCA+AGG - chr7_3:16378430-16378449 Msa1094500:CDS 35.0%
AACCTTGATTTAGTGCTTCT+AGG + chr7_3:16378435-16378454 None:intergenic 35.0%
! AGCACTTCGTGAACAAATTT+TGG + chr7_3:16378564-16378583 None:intergenic 35.0%
GATTCTAAATACTTGAGGAG+AGG - chr7_3:16378594-16378613 Msa1094500:CDS 35.0%
AAATACTTGAGGAGAGGTAA+TGG - chr7_3:16378600-16378619 Msa1094500:CDS 35.0%
TCTTCATGGAAAACATTGCT+AGG - chr7_3:16378667-16378686 Msa1094500:CDS 35.0%
GAAATGAATCCCTTCCCATT+TGG + chr7_3:16376810-16376829 None:intergenic 40.0%
! ATCTATTTCTTCCCAAGTGG+CGG + chr7_3:16377086-16377105 None:intergenic 40.0%
TTTGCATTTGAACCCTGCAA+CGG + chr7_3:16377355-16377374 None:intergenic 40.0%
! TAGTTGCGGCTTTGTGAATA+CGG - chr7_3:16377441-16377460 Msa1094500:intron 40.0%
ATAGTGGCTACAACATCATG+AGG - chr7_3:16377577-16377596 Msa1094500:CDS 40.0%
AAAGAGGAGAAACAATGCAG+TGG - chr7_3:16377714-16377733 Msa1094500:CDS 40.0%
! TTAGATAATGGTTCACCAGG+AGG + chr7_3:16377822-16377841 None:intergenic 40.0%
!! GCAAAACATTGTTGGTTTCG+TGG - chr7_3:16377925-16377944 Msa1094500:intron 40.0%
TGTGTCATATCACATGCTCT+TGG - chr7_3:16377989-16378008 Msa1094500:intron 40.0%
CTGCTATGGATAAGATACAC+AGG + chr7_3:16378154-16378173 None:intergenic 40.0%
CACTTCAGATTTGACTGCTA+TGG + chr7_3:16378168-16378187 None:intergenic 40.0%
CATAGCAGTCAAATCTGAAG+TGG - chr7_3:16378166-16378185 Msa1094500:intron 40.0%
! CCAGGAAGAACTGTAAGTTT+TGG + chr7_3:16378202-16378221 None:intergenic 40.0%
CCAAAACTTACAGTTCTTCC+TGG - chr7_3:16378199-16378218 Msa1094500:intron 40.0%
ATAAAGTGGCATTCACACCT+AGG - chr7_3:16378276-16378295 Msa1094500:intron 40.0%
AAAACATTCAGAGAAAGCCC+TGG - chr7_3:16378381-16378400 Msa1094500:CDS 40.0%
! GAAAGCACAACATTTGCTTG+AGG - chr7_3:16378629-16378648 Msa1094500:CDS 40.0%
GAAATGAATCCCTTCCCATT+TGG + chr7_3:16376810-16376829 None:intergenic 40.0%
! ATCTATTTCTTCCCAAGTGG+CGG + chr7_3:16377086-16377105 None:intergenic 40.0%
TTTGCATTTGAACCCTGCAA+CGG + chr7_3:16377355-16377374 None:intergenic 40.0%
! TAGTTGCGGCTTTGTGAATA+CGG - chr7_3:16377441-16377460 Msa1094500:intron 40.0%
ATAGTGGCTACAACATCATG+AGG - chr7_3:16377577-16377596 Msa1094500:CDS 40.0%
AAAGAGGAGAAACAATGCAG+TGG - chr7_3:16377714-16377733 Msa1094500:CDS 40.0%
! TTAGATAATGGTTCACCAGG+AGG + chr7_3:16377822-16377841 None:intergenic 40.0%
!! GCAAAACATTGTTGGTTTCG+TGG - chr7_3:16377925-16377944 Msa1094500:intron 40.0%
TGTGTCATATCACATGCTCT+TGG - chr7_3:16377989-16378008 Msa1094500:intron 40.0%
CTGCTATGGATAAGATACAC+AGG + chr7_3:16378154-16378173 None:intergenic 40.0%
CACTTCAGATTTGACTGCTA+TGG + chr7_3:16378168-16378187 None:intergenic 40.0%
CATAGCAGTCAAATCTGAAG+TGG - chr7_3:16378166-16378185 Msa1094500:intron 40.0%
! CCAGGAAGAACTGTAAGTTT+TGG + chr7_3:16378202-16378221 None:intergenic 40.0%
CCAAAACTTACAGTTCTTCC+TGG - chr7_3:16378199-16378218 Msa1094500:intron 40.0%
ATAAAGTGGCATTCACACCT+AGG - chr7_3:16378276-16378295 Msa1094500:intron 40.0%
AAAACATTCAGAGAAAGCCC+TGG - chr7_3:16378381-16378400 Msa1094500:CDS 40.0%
! GAAAGCACAACATTTGCTTG+AGG - chr7_3:16378629-16378648 Msa1094500:CDS 40.0%
! ATAGTTGGCTCTTCTTCAGC+CGG + chr7_3:16376986-16377005 None:intergenic 45.0%
GTTGCAGGGTTCAAATGCAA+AGG - chr7_3:16377354-16377373 Msa1094500:intron 45.0%
ACTCATTCTACTGATCCTCC+TGG - chr7_3:16377804-16377823 Msa1094500:intron 45.0%
GATCTTTCTTACGTGAGGGA+TGG + chr7_3:16377953-16377972 None:intergenic 45.0%
ATATCACATGCTCTTGGAGC+AGG - chr7_3:16377995-16378014 Msa1094500:intron 45.0%
AGCTCATGACAAGAACACCT+AGG + chr7_3:16378296-16378315 None:intergenic 45.0%
CGTCAAGTATCAAGAGTCCA+GGG + chr7_3:16378401-16378420 None:intergenic 45.0%
TCGTCAAGTATCAAGAGTCC+AGG + chr7_3:16378402-16378421 None:intergenic 45.0%
! ATAGTTGGCTCTTCTTCAGC+CGG + chr7_3:16376986-16377005 None:intergenic 45.0%
GTTGCAGGGTTCAAATGCAA+AGG - chr7_3:16377354-16377373 Msa1094500:intron 45.0%
ACTCATTCTACTGATCCTCC+TGG - chr7_3:16377804-16377823 Msa1094500:intron 45.0%
GATCTTTCTTACGTGAGGGA+TGG + chr7_3:16377953-16377972 None:intergenic 45.0%
ATATCACATGCTCTTGGAGC+AGG - chr7_3:16377995-16378014 Msa1094500:intron 45.0%
AGCTCATGACAAGAACACCT+AGG + chr7_3:16378296-16378315 None:intergenic 45.0%
CGTCAAGTATCAAGAGTCCA+GGG + chr7_3:16378401-16378420 None:intergenic 45.0%
TCGTCAAGTATCAAGAGTCC+AGG + chr7_3:16378402-16378421 None:intergenic 45.0%
!! CAAAAAAATATTATAAATAT+AGG + chr7_3:16377168-16377187 None:intergenic 5.0%
!!! CTATATTTATAATATTTTTT+TGG - chr7_3:16377166-16377185 Msa1094500:intron 5.0%
!! ATATATATAAATAATTTATC+AGG + chr7_3:16377667-16377686 None:intergenic 5.0%
!! AATTATTTATATATATAAGT+AGG - chr7_3:16377671-16377690 Msa1094500:CDS 5.0%
!! CAAAAAAATATTATAAATAT+AGG + chr7_3:16377168-16377187 None:intergenic 5.0%
!!! CTATATTTATAATATTTTTT+TGG - chr7_3:16377166-16377185 Msa1094500:intron 5.0%
!! ATATATATAAATAATTTATC+AGG + chr7_3:16377667-16377686 None:intergenic 5.0%
!! AATTATTTATATATATAAGT+AGG - chr7_3:16377671-16377690 Msa1094500:CDS 5.0%
! TTGATGACTTGTCCGCCACT+TGG - chr7_3:16377071-16377090 Msa1094500:CDS 50.0%
! TGATGACTTGTCCGCCACTT+GGG - chr7_3:16377072-16377091 Msa1094500:CDS 50.0%
! AGGTCTTCTTGATGGACCTG+AGG - chr7_3:16377123-16377142 Msa1094500:intron 50.0%
AATCGATTGCCACACACCTC+AGG + chr7_3:16377142-16377161 None:intergenic 50.0%
CCAATAGGTCTTGGAACACC+AGG + chr7_3:16378220-16378239 None:intergenic 50.0%
CCTGGTGTTCCAAGACCTAT+TGG - chr7_3:16378217-16378236 Msa1094500:intron 50.0%
CACAACATTTGCTTGAGGCG+CGG - chr7_3:16378634-16378653 Msa1094500:CDS 50.0%
! TTGATGACTTGTCCGCCACT+TGG - chr7_3:16377071-16377090 Msa1094500:CDS 50.0%
! TGATGACTTGTCCGCCACTT+GGG - chr7_3:16377072-16377091 Msa1094500:CDS 50.0%
! AGGTCTTCTTGATGGACCTG+AGG - chr7_3:16377123-16377142 Msa1094500:intron 50.0%
AATCGATTGCCACACACCTC+AGG + chr7_3:16377142-16377161 None:intergenic 50.0%
CCAATAGGTCTTGGAACACC+AGG + chr7_3:16378220-16378239 None:intergenic 50.0%
CCTGGTGTTCCAAGACCTAT+TGG - chr7_3:16378217-16378236 Msa1094500:intron 50.0%
CACAACATTTGCTTGAGGCG+CGG - chr7_3:16378634-16378653 Msa1094500:CDS 50.0%
! CTTGATGGACCTGAGGTGTG+TGG - chr7_3:16377130-16377149 Msa1094500:intron 55.0%
GCGGCGAAGGAGAATCTTCA+TGG - chr7_3:16378653-16378672 Msa1094500:CDS 55.0%
! CTTGATGGACCTGAGGTGTG+TGG - chr7_3:16377130-16377149 Msa1094500:intron 55.0%
GCGGCGAAGGAGAATCTTCA+TGG - chr7_3:16378653-16378672 Msa1094500:CDS 55.0%
ATTTGCTTGAGGCGCGGCGA+AGG - chr7_3:16378640-16378659 Msa1094500:CDS 60.0%
ATTTGCTTGAGGCGCGGCGA+AGG - chr7_3:16378640-16378659 Msa1094500:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 16376794 16378710 16376794 ID=Msa1094500;Name=Msa1094500
chr7_3 mRNA 16376794 16378710 16376794 ID=Msa1094500-mRNA-1;Parent=Msa1094500;Name=Msa1094500-mRNA-1;_AED=0.30;_eAED=0.37;_QI=0|0|0|1|1|1|3|0|325
chr7_3 exon 16378380 16378710 16378380 ID=Msa1094500-mRNA-1:exon:3653;Parent=Msa1094500-mRNA-1
chr7_3 exon 16377459 16377786 16377459 ID=Msa1094500-mRNA-1:exon:3652;Parent=Msa1094500-mRNA-1
chr7_3 exon 16376794 16377112 16376794 ID=Msa1094500-mRNA-1:exon:3651;Parent=Msa1094500-mRNA-1
chr7_3 CDS 16378380 16378710 16378380 ID=Msa1094500-mRNA-1:cds;Parent=Msa1094500-mRNA-1
chr7_3 CDS 16377459 16377786 16377459 ID=Msa1094500-mRNA-1:cds;Parent=Msa1094500-mRNA-1
chr7_3 CDS 16376794 16377112 16376794 ID=Msa1094500-mRNA-1:cds;Parent=Msa1094500-mRNA-1
Gene Sequence

>Msa1094500

ATGGATTTTGAAACCAAATGGGAAGGGATTCATTTCAAAAAAGGTGCTCAAACAAAAAGATATCATTCAATTTGTTTGTCAAACAAAATTCACAGTCATGAAGAACAAGATAATAATGGTGATAGAACGTTAAATGTTGATGCACACAAGGAAATAATTGGCACTTACATGAAGAATTTGGATAGTCTTCCGGCTGAAGAAGAGCCAACTATTAATGAAGAAACAAACAAGTTTGAGGAAGAAAAAATGTCGAAAAGAGATGATGAGGAAGAAAATCTTGATGACTTGTCCGCCACTTGGGAAGAAATAGATACTGCTTTAATAGCAAAAGAGGAGAAACAATGCAGTGGAGAAGATACTAATGAACAGAAATCAAAGCAAAAAGTTTATGAAGATGAGAACAATGATATGTTCTCAACTCATTCTACTGATCCTCCTGGTGAACCATTATCTAAAGAAGAAGACAATGTTTGGGAATTGATTCCTGATTCAAAGAAAAGATGCATACACACCCGAAAAAAGTTTTTGAATTTCTTTCGCAAAACATTGTTGGTTTCGTGGAACCATCCCTCACGTAAGAAAGATCTGAAACAAGTGTTGGATGTGTCATATCACATGCTCTTGGAGCAGGAAAAACCTTTCTCAACATTTCTTTTCTTAGAAAGCCCTGGACTCTTGATACTTGACGAAGCTCATAATCCTAGAAGCACTAAATCAAGGTTGAAGAAATGCTTAATGGAACTTTCAACAACATTAAGAATATTACTTTCTAGCACTCTTTTCCAGAGCAATTTTTGTGAATATTTCAACAATCTTTGTTTAGCAAGACCAAAATTTGTTCACGAAGTGCTACGAGAGTTAGATTCTAAATACTTGAGGAGAGGTAATGGAGAGAAGAAAGCACAACATTTGCTTGAGGCGCGGCGAAGGAGAATCTTCATGGAAAACATTGCTAGGAAAATCAACTCACAATCATGA
Protein sequence

>Msa1094500

MDFETKWEGIHFKKGAQTKRYHSICLSNKIHSHEEQDNNGDRTLNVDAHKEIIGTYMKNLDSLPAEEEPTINEETNKFEEEKMSKRDDEEENLDDLSATWEEIDTALIAKEEKQCSGEDTNEQKSKQKVYEDENNDMFSTHSTDPPGEPLSKEEDNVWELIPDSKKRCIHTRKKFLNFFRKTLLVSWNHPSRKKDLKQVLDVSYHMLLEQEKPFSTFLFLESPGLLILDEAHNPRSTKSRLKKCLMELSTTLRILLSSTLFQSNFCEYFNNLCLARPKFVHEVLRELDSKYLRRGNGEKKAQHLLEARRRRIFMENIARKINSQS*