AlfalfaGEDB | Msa1103230

M. sativa cultivar zhongmu-4 / Msa1103230

KEGG: K09420|K09422
GO: GO:0000003 GO:0000278 GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006109 GO:0006355 GO:0006357 GO:0006935 GO:0007049 GO:0007275 GO:0008150 GO:0009553 GO:0009605 GO:0009790 GO:0009791 GO:0009793 GO:0009856 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009960 GO:0009987 GO:0010154 GO:0010183 GO:0010228 GO:0010262 GO:0010439 GO:0010468 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010675 GO:0019216 GO:0019217 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0040011 GO:0042221 GO:0042304 GO:0042330 GO:0042592 GO:0042762 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043255 GO:0043455 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0044706 GO:0045595 GO:0045691 GO:0045697 GO:0045723 GO:0045834 GO:0045893 GO:0045923 GO:0045935 GO:0045995 GO:0046889 GO:0046890 GO:0048229 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048856 GO:0048868 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0050896 GO:0050918 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051704 GO:0055081 GO:0055088 GO:0055089 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0080050 GO:0080090 GO:0097159 GO:0140110 GO:1900376 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1904095 GO:2000014 GO:2000026 GO:2000034 GO:2000112 GO:2000241 GO:2000242 GO:2000692 GO:2001141 GO:2001279 GO:2001280
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1103230	RHN45605.1	87.594	266	33	0	6	271	85	350	3.56e-167	476

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1103230	sp\|Q9LVW4\|MY118_ARATH	51.020	98	45	2	177	271	143	240	3.91e-21	95.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1103230	A0A396H398	87.594	266	33	0	6	271	85	350	1.70e-167	476

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TFs/TRs:

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Gene ID	Type	Classification
Msa1103230	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1103230	MtrunA17_Chr7g0233161	87.594	266	33	0	6	271	85	350	3.28e-171	476
Msa1103230	MtrunA17_Chr4g0046181	69.776	268	81	0	4	271	5	272	3.73e-121	347
Msa1103230	MtrunA17_Chr7g0228531	65.660	265	90	1	7	271	8	271	1.95e-119	348
Msa1103230	MtrunA17_Chr7g0228481	63.774	265	95	1	7	271	8	271	2.71e-113	333
Msa1103230	MtrunA17_Chr7g0228401	43.030	330	122	3	4	271	5	330	1.78e-75	238
Msa1103230	MtrunA17_Chr7g0233061	35.955	267	122	6	8	271	9	229	1.52e-39	140
Msa1103230	MtrunA17_Chr3g0117511	70.690	58	17	0	214	271	177	234	1.08e-20	90.9
Msa1103230	MtrunA17_Chr3g0104501	69.811	53	16	0	219	271	132	184	2.48e-20	86.3
Msa1103230	MtrunA17_Chr5g0429661	67.925	53	17	0	219	271	121	173	1.26e-19	87.0
Msa1103230	MtrunA17_Chr4g0058991	67.925	53	17	0	219	271	208	260	2.22e-19	87.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1103230	AT3G27785.1	51.020	98	45	2	177	271	143	240	3.98e-22	95.5
Msa1103230	AT4G18770.1	73.585	53	14	0	219	271	216	268	1.19e-21	94.0
Msa1103230	AT5G58850.1	39.231	130	72	1	142	271	34	156	3.11e-20	89.7
Msa1103230	AT5G11050.1	65.455	55	19	0	217	271	102	156	3.09e-19	87.0
Msa1103230	AT5G11510.3	59.615	52	21	0	219	270	80	131	1.34e-16	80.1
Msa1103230	AT5G11510.1	59.615	52	21	0	219	270	80	131	1.34e-16	80.1
Msa1103230	AT5G11510.4	59.615	52	21	0	219	270	80	131	1.34e-16	80.1
Msa1103230	AT5G11510.2	59.615	52	21	0	219	270	80	131	2.28e-16	79.3
Msa1103230	AT5G11510.5	59.615	52	21	0	219	270	80	131	2.41e-16	79.0
Msa1103230	AT5G40360.1	61.538	52	20	0	220	271	158	209	4.18e-16	77.4

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Find 76 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCCTTATGCGAAATCA+GGG	0.555864	7_3:-38678936	Msa1103230:CDS
AAATCAATGCAATCAACTTA+AGG	0.438416	7_3:-38678836	Msa1103230:CDS
AAATCCAGAGAAAATGATCA+AGG	0.549710	7_3:-38678436	Msa1103230:CDS
AAATTCTCTCTGATACCCAT+TGG	0.560239	7_3:+38678705	None:intergenic
AAGAATGAGACCTTAATGTC+TGG	0.513472	7_3:+38678042	None:intergenic
AAGGATCTCAATCAAATCAA+GGG	0.611928	7_3:-38678352	Msa1103230:CDS
AAGTTTATGAAAGGCAGAAC+TGG	0.415305	7_3:-38678102	Msa1103230:CDS
AATAAGATGGAAGTCATACA+TGG	0.614721	7_3:-38678643	Msa1103230:CDS
AATGAATATGGGCCGTTTGA+TGG	0.356823	7_3:-38679141	None:intergenic
AATGGACTTCAAATGAGGAT+CGG	0.644601	7_3:-38678327	Msa1103230:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATCCTTATGCGAAATC+AGG	-	chr7_3:38678250-38678269	Msa1103230:intron	30.0%
AAAATCCTTATGCGAAATCA+GGG	-	chr7_3:38678251-38678270	Msa1103230:intron	30.0%
ACTTCCATCTTATTATGGTT+TGG	+	chr7_3:38678538-38678557	None:intergenic	30.0%
GTATGACTTCCATCTTATTA+TGG	+	chr7_3:38678543-38678562	None:intergenic	30.0%
AATAAGATGGAAGTCATACA+TGG	-	chr7_3:38678544-38678563	Msa1103230:CDS	30.0%
TTAACACTAGCAAAGGAATT+TGG	-	chr7_3:38678572-38678591	Msa1103230:CDS	30.0%
TAACACTAGCAAAGGAATTT+GGG	-	chr7_3:38678573-38678592	Msa1103230:CDS	30.0%
AAATCCAGAGAAAATGATCA+AGG	-	chr7_3:38678751-38678770	Msa1103230:CDS	30.0%
AAGGATCTCAATCAAATCAA+GGG	-	chr7_3:38678835-38678854	Msa1103230:CDS	30.0%
AAAAATCCTTATGCGAAATC+AGG	-	chr7_3:38678250-38678269	Msa1103230:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	38678053	38679156	38678053	ID=Msa1103230;Name=Msa1103230
chr7_3	mRNA	38678053	38679156	38678053	ID=Msa1103230-mRNA-1;Parent=Msa1103230;Name=Msa1103230-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0\|0\|0\|0.66\|0.5\|0.33\|3\|0\|271
chr7_3	exon	38679142	38679156	38679142	ID=Msa1103230-mRNA-1:exon:7849;Parent=Msa1103230-mRNA-1
chr7_3	exon	38678328	38678998	38678328	ID=Msa1103230-mRNA-1:exon:7848;Parent=Msa1103230-mRNA-1
chr7_3	exon	38678053	38678179	38678053	ID=Msa1103230-mRNA-1:exon:7847;Parent=Msa1103230-mRNA-1
chr7_3	CDS	38679142	38679156	38679142	ID=Msa1103230-mRNA-1:cds;Parent=Msa1103230-mRNA-1
chr7_3	CDS	38678328	38678998	38678328	ID=Msa1103230-mRNA-1:cds;Parent=Msa1103230-mRNA-1
chr7_3	CDS	38678053	38678179	38678053	ID=Msa1103230-mRNA-1:cds;Parent=Msa1103230-mRNA-1

Gene Sequence

>Msa1103230

ATGGGCCGTTTGATGTTTGCAGAAAAAATTCCTTATCTTTCAAGGGATTTTCCTAAAAATCCTTATGCGAAATCAGGGATCCCCATAATGGTTCCTTCTCAACTAGGTTCAAATCTTTCACCTCCAACATCACATTACACCACATATTTGTACCAAAATCAATGCAATCAACTTAAGGAACATCCTTTGAATGAGGTTAACCATCACTCTAGTTTTTCTGCAATGGTTCCTTCCCCCATCAGCCCATTCCCTTTGATCCCAACCCATCGGTACAAGGCTACTTACACCAATGGGTATCAGAGAGAATTTATCAGTGCTCGAAATCCAATGATCTTTGCACCAAACCATAATAAGATGGAAGTCATACATGGTCGTCTTAACACTAGCAAAGGAATTTGGGATTTATCTACAAAAAATTTCTTTCAATATGGTGAAACTTCACAACCTCGGGTAAGTCCCTCTCCATCACCATCACTAGCTTATGATGCACATCAATCTGTGTCAATTAATCCTGAGCTTCAAGGAGATCTTTCATGCAATGATGGAATTGGAAACAAATCCAGAGAAAATGATCAAGGATTCGTGCTCAGTGTCCAAAAGCGGCTGAAGAGGATTCAAAAGAATAATGAAATTCAACACAAGGATCTCAATCAAATCAAGGGACAATGGACTTCAAATGAGGATCGGGTTCTTGTCCAATTGGTGGATCACTTTGGGCTAAGAAAATGGTCCTGTATTGCTAAGTTTATGAAAGGCAGAACTGGAAAACAGTGCAGAGAGAGATGGAATAACCATCTTCGACCAGACATTAAG

Protein sequence

>Msa1103230

MGRLMFAEKIPYLSRDFPKNPYAKSGIPIMVPSQLGSNLSPPTSHYTTYLYQNQCNQLKEHPLNEVNHHSSFSAMVPSPISPFPLIPTHRYKATYTNGYQREFISARNPMIFAPNHNKMEVIHGRLNTSKGIWDLSTKNFFQYGETSQPRVSPSPSPSLAYDAHQSVSINPELQGDLSCNDGIGNKSRENDQGFVLSVQKRLKRIQKNNEIQHKDLNQIKGQWTSNEDRVLVQLVDHFGLRKWSCIAKFMKGRTGKQCRERWNNHLRPDIK