AlfalfaGEDB | Msa1103940

M. sativa cultivar zhongmu-4 / Msa1103940

KEGG: K11420
GO: -
PFAM: PF05033.19

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1103940	AES79015.2	76.000	100	24	0	531	630	431	530	5.90e-40	166
Msa1103940	AES79015.2	85.080	563	27	3	1	517	1	552	0.0	964

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1103940	sp\|Q8GZB6\|SUVH4_ARATH	54.518	332	146	4	190	517	290	620	3.30e-121	376
Msa1103940	sp\|Q8GZB6\|SUVH4_ARATH	56.383	94	38	2	541	633	510	601	2.30e-22	105

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1103940	G7KY38	76.000	100	24	0	531	630	431	530	2.82e-40	166
Msa1103940	G7KY38	85.080	563	27	3	1	517	1	552	0.0	964

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TFs/TRs:

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Gene ID	Type	Classification
Msa1103940	TR	SET

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1103940	MtrunA17_Chr7g0234361	78.652	356	18	4	1	310	1	344	0.0	531
Msa1103940	MtrunA17_Chr6g0476121	56.024	332	142	3	190	517	308	639	1.29e-122	377
Msa1103940	MtrunA17_Chr6g0476121	57.778	90	38	0	541	630	528	617	3.05e-23	104
Msa1103940	MtrunA17_Chr7g0252211	56.024	332	142	3	190	517	422	753	6.74e-122	379
Msa1103940	MtrunA17_Chr7g0252211	57.778	90	38	0	541	630	642	731	2.97e-23	105
Msa1103940	MtrunA17_Chr6g0476111	55.723	332	143	3	190	517	359	690	1.27e-121	376
Msa1103940	MtrunA17_Chr6g0476111	57.778	90	38	0	541	630	579	668	4.30e-23	104
Msa1103940	MtrunA17_Chr5g0399801	46.316	285	139	8	235	516	811	1084	6.06e-67	237
Msa1103940	MtrunA17_Chr5g0399801	51.515	66	32	0	565	630	998	1063	4.19e-14	76.3
Msa1103940	MtrunA17_Chr4g0003501	41.801	311	163	7	210	516	654	950	3.09e-62	223

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1103940	AT5G13960.2	54.518	332	146	4	190	517	156	486	1.10e-122	373
Msa1103940	AT5G13960.2	56.383	94	38	2	541	633	376	467	1.61e-23	105
Msa1103940	AT5G13960.1	54.518	332	146	4	190	517	290	620	3.36e-122	376
Msa1103940	AT5G13960.1	56.383	94	38	2	541	633	510	601	2.34e-23	105
Msa1103940	AT2G22740.2	39.610	308	162	9	220	517	493	786	3.01e-53	196
Msa1103940	AT2G22740.2	45.070	71	39	0	568	638	702	772	4.54e-12	69.7
Msa1103940	AT2G22740.1	39.610	308	162	9	220	517	493	786	3.01e-53	196
Msa1103940	AT2G22740.1	45.070	71	39	0	568	638	702	772	4.54e-12	69.7
Msa1103940	AT2G35160.5	34.659	352	185	9	173	516	439	753	6.74e-53	194
Msa1103940	AT2G35160.2	34.659	352	185	9	173	516	475	789	1.50e-52	194

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Find 128 sgRNAs with CRISPR-Local

Find 392 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGCTTCAATGTCATTG+TGG	0.646249	7_3:-40395203	Msa1103940:CDS
AAACTCTTTCTTCTTCTCCA+AGG	0.531848	7_3:-40397519	Msa1103940:intron
AACCCTAGATGTTTCTCTCA+AGG	0.580795	7_3:+40399347	None:intergenic
AAGAGGAATACCTGATAGGG+AGG	0.649275	7_3:+40396121	None:intergenic
AAGGTTGGGCGGTAAGAACA+TGG	0.558115	7_3:-40396594	Msa1103940:CDS
AAGTTTGGTATCAACATCAA+AGG	0.550778	7_3:-40399598	Msa1103940:CDS
AATATTCGTATTACCTGATA+GGG	0.446675	7_3:+40393531	None:intergenic
AATGATTCACTTGTCTTTGA+TGG	0.221527	7_3:-40399393	Msa1103940:CDS
AATGTTATTGTATGAAAACT+CGG	0.621218	7_3:+40397001	None:intergenic
ACAAAGAGGAATACCTGATA+GGG	0.490411	7_3:+40396118	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTGAATCTTAGAGTGATTC+TGG	+	chr7_3:40393320-40393339	None:intergenic	30.0%
TATAACATTTACAACAGCAG+CGG	-	chr7_3:40393339-40393358	Msa1103940:intron	30.0%
CATGTTGAAAAGATTGAACT+TGG	-	chr7_3:40393381-40393400	Msa1103940:intron	30.0%
AAGTTTGGTATCAACATCAA+AGG	-	chr7_3:40393416-40393435	Msa1103940:intron	30.0%
CTCTTATGGTTAATATCATC+TGG	+	chr7_3:40393474-40393493	None:intergenic	30.0%
GATGATATTAACCATAAGAG+AGG	-	chr7_3:40393474-40393493	Msa1103940:intron	30.0%
AATGATTCACTTGTCTTTGA+TGG	-	chr7_3:40393621-40393640	Msa1103940:CDS	30.0%
TAGAAAAAGTTTAGACGAGT+TGG	-	chr7_3:40393848-40393867	Msa1103940:intron	30.0%
TGTGAACTCTATTAATATGG+TGG	-	chr7_3:40394582-40394601	Msa1103940:intron	30.0%
GTATCTGATAAATAAGCTTC+GGG	-	chr7_3:40394951-40394970	Msa1103940:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	40393252	40399784	40393252	ID=Msa1103940;Name=Msa1103940
chr7_3	mRNA	40393252	40399784	40393252	ID=Msa1103940-mRNA-1;Parent=Msa1103940;Name=Msa1103940-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0\|0\|0\|0.84\|1\|1\|13\|0\|639
chr7_3	exon	40399323	40399784	40399323	ID=Msa1103940-mRNA-1:exon:8243;Parent=Msa1103940-mRNA-1
chr7_3	exon	40398619	40398693	40398619	ID=Msa1103940-mRNA-1:exon:8242;Parent=Msa1103940-mRNA-1
chr7_3	exon	40398037	40398138	40398037	ID=Msa1103940-mRNA-1:exon:8241;Parent=Msa1103940-mRNA-1
chr7_3	exon	40397520	40397569	40397520	ID=Msa1103940-mRNA-1:exon:8240;Parent=Msa1103940-mRNA-1
chr7_3	exon	40397331	40397419	40397331	ID=Msa1103940-mRNA-1:exon:8239;Parent=Msa1103940-mRNA-1
chr7_3	exon	40396996	40397146	40396996	ID=Msa1103940-mRNA-1:exon:8238;Parent=Msa1103940-mRNA-1
chr7_3	exon	40396726	40396831	40396726	ID=Msa1103940-mRNA-1:exon:8237;Parent=Msa1103940-mRNA-1
chr7_3	exon	40396453	40396635	40396453	ID=Msa1103940-mRNA-1:exon:8236;Parent=Msa1103940-mRNA-1
chr7_3	exon	40396132	40396365	40396132	ID=Msa1103940-mRNA-1:exon:8235;Parent=Msa1103940-mRNA-1
chr7_3	exon	40395189	40395285	40395189	ID=Msa1103940-mRNA-1:exon:8234;Parent=Msa1103940-mRNA-1
chr7_3	exon	40394480	40394553	40394480	ID=Msa1103940-mRNA-1:exon:8233;Parent=Msa1103940-mRNA-1
chr7_3	exon	40393545	40393778	40393545	ID=Msa1103940-mRNA-1:exon:8232;Parent=Msa1103940-mRNA-1
chr7_3	exon	40393252	40393314	40393252	ID=Msa1103940-mRNA-1:exon:8231;Parent=Msa1103940-mRNA-1
chr7_3	CDS	40399323	40399784	40399323	ID=Msa1103940-mRNA-1:cds;Parent=Msa1103940-mRNA-1
chr7_3	CDS	40398619	40398693	40398619	ID=Msa1103940-mRNA-1:cds;Parent=Msa1103940-mRNA-1
chr7_3	CDS	40398037	40398138	40398037	ID=Msa1103940-mRNA-1:cds;Parent=Msa1103940-mRNA-1
chr7_3	CDS	40397520	40397569	40397520	ID=Msa1103940-mRNA-1:cds;Parent=Msa1103940-mRNA-1
chr7_3	CDS	40397331	40397419	40397331	ID=Msa1103940-mRNA-1:cds;Parent=Msa1103940-mRNA-1
chr7_3	CDS	40396996	40397146	40396996	ID=Msa1103940-mRNA-1:cds;Parent=Msa1103940-mRNA-1
chr7_3	CDS	40396726	40396831	40396726	ID=Msa1103940-mRNA-1:cds;Parent=Msa1103940-mRNA-1
chr7_3	CDS	40396453	40396635	40396453	ID=Msa1103940-mRNA-1:cds;Parent=Msa1103940-mRNA-1
chr7_3	CDS	40396132	40396365	40396132	ID=Msa1103940-mRNA-1:cds;Parent=Msa1103940-mRNA-1
chr7_3	CDS	40395189	40395285	40395189	ID=Msa1103940-mRNA-1:cds;Parent=Msa1103940-mRNA-1
chr7_3	CDS	40394480	40394553	40394480	ID=Msa1103940-mRNA-1:cds;Parent=Msa1103940-mRNA-1
chr7_3	CDS	40393545	40393778	40393545	ID=Msa1103940-mRNA-1:cds;Parent=Msa1103940-mRNA-1
chr7_3	CDS	40393252	40393314	40393252	ID=Msa1103940-mRNA-1:cds;Parent=Msa1103940-mRNA-1

Gene Sequence

>Msa1103940

ATGGAGGCTACTAAGAGTTCTGAAAATGAGGCTATGATGGTACCAACAAGAGTGAGTTCAAGACTCCAGAATCACTCTAAGATTCAATATAACATTTACAACAGCAGCGGCGACAAAGTATTTGCCCAACATGTTGAAAAGATTGAACTTGGAAGTAAAGAGGAAAGTTTGGTATCAACATCAAAGGTTTGTTCACAACTTCAAAATCGAAACGAAATTCCAGATGATATTAACCATAAGAGAGGCATGATCAGTATTGAAGACAATATTGTCGAGAAGAGAACCAAGCTCAACTCTTTGAGAAGAAAACCTTGCTCTCATTCAAGTTCAAGAAGTGGTTCAAGAATCTCTCGTTCAAGATCAACAAGTAATGATTCACTTGTCTTTGATGGACTTGACTTTAGGGGCATTTTGTCCTTGAGAGAAACATCTAGGGTTGCCAATCTTCGTTGCCTTGCTAAGCAGGAAGAGAAGAATCAAAGCAAGAATCGACCCAAGATAGGGAAAAATATTGCGACACTTTTGAGGGCAAGCAATGTAGAGAAAAATGGCATTGATAACGAATACGTTCCTGGAATCACTACTTCGAAGTATCTGATAAATAAGCTTCGGGAACTTGCTAAAGTGGGAACATACAAGGTTCATTTAATGGAAGGGCAAGTCTCTAAAACTCTTTCTTCTTCTCCAAGTTTGGTATGCAAGGACATTAGCAATGGACAAGAAGCTATCAGCATTATTGCAACCAATGATTTTGATGATCCTCCAGTTGCACCCACAGGTTTTGAATACATTACATCAAACAAATTTTCACCAAGCATTGAAGTTCCATCTAGTGCTGCGGGATGCAATTGCAAGGGAAGTTGTAGAACCAAACGATGTTCTTGTGCTAATCATAATGGCTCCGAGTTTTCATACAATAACATTGGCCGATTGATCGAGCCTCTAGACATCGTGGTCGAATGTGGACCGCAATGTGGATGCGGACCTAAATGTGGAAACAAAGTTTCTCAACAGGGCTTAACTTACCGACTTGAGGTTTATCGTACTCCCAAGAAAGGTTGGGCGGTAAGAACATGGGACTTTATCCCATCAGGTGCACCAGTTGTCGAGTATATTGGTGTACTAAGTAGAGACGATGAGCTAGGAAGTGCCAACGGAAATGATTATATTTTCGATATTGATTGTTTGCACACAATCAATGGTGTAGATGGAAGAGAGAGACGGTTGGGTAATGTACCATTGCCTATAAGCAATTTATCTGAAAAAAGGGACGAGTTAATGGAAAAAGATCCTGAATATTGCATAGATGCTGGTTCTTTTGGAAATGTTTCAAGATTTATCAACCATGGATGTGAACCAAACCTCTTTGTACAATGTGTTCTCAGCAGCCACAGAGATCCTAGACTAACTCGTGTAGTGCTATTTGCAGCAGAAGACATACCTCCCTATCAGGAACTCACCTATGACTATGGTTACACACTCGATAGTGTGTCTGATTCTGATGGAAAAATCAAACAGCTTCAATGTCATTGTGGTGCAAAGGAATGTCGCGCCTCTAAATTAGAGGCACTGCGAAGGGTCTTGATGCACTTCCACAAGGTGTACCCTGATGAAATGGAAAAGAGACGGTTGGGTAATGTACCATTGCCTATACACAATTTATCTGAAAAAAGGGACAAGTTAATGGAAAAAGATCCTGAATATTGCATAGATGCTGGTTCTTTTGGTAATGTTTCAAGATTTATCAACCATGGATGTGAACCAAACCTCTTTGTACAATGTGTTCTCAGCAGCCACAGAGATCCTAGACTAGCTCGTGTGGTGCTATTTGCAGCATGTGACATACCTCCCTATCAGTCTAAGGCGTTGTCGTTTCATTTGACAACGGATTTGTTGCATACAGGAAAACGCAACAGATAA

Protein sequence

>Msa1103940

MEATKSSENEAMMVPTRVSSRLQNHSKIQYNIYNSSGDKVFAQHVEKIELGSKEESLVSTSKVCSQLQNRNEIPDDINHKRGMISIEDNIVEKRTKLNSLRRKPCSHSSSRSGSRISRSRSTSNDSLVFDGLDFRGILSLRETSRVANLRCLAKQEEKNQSKNRPKIGKNIATLLRASNVEKNGIDNEYVPGITTSKYLINKLRELAKVGTYKVHLMEGQVSKTLSSSPSLVCKDISNGQEAISIIATNDFDDPPVAPTGFEYITSNKFSPSIEVPSSAAGCNCKGSCRTKRCSCANHNGSEFSYNNIGRLIEPLDIVVECGPQCGCGPKCGNKVSQQGLTYRLEVYRTPKKGWAVRTWDFIPSGAPVVEYIGVLSRDDELGSANGNDYIFDIDCLHTINGVDGRERRLGNVPLPISNLSEKRDELMEKDPEYCIDAGSFGNVSRFINHGCEPNLFVQCVLSSHRDPRLTRVVLFAAEDIPPYQELTYDYGYTLDSVSDSDGKIKQLQCHCGAKECRASKLEALRRVLMHFHKVYPDEMEKRRLGNVPLPIHNLSEKRDKLMEKDPEYCIDAGSFGNVSRFINHGCEPNLFVQCVLSSHRDPRLARVVLFAACDIPPYQSKALSFHLTTDLLHTGKRNR*