AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1110240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1110240 MtrunA17_Chr1g0208561 62.719 228 28 3 1 171 1 228 2.15e-90 263
Msa1110240 MtrunA17_Chr3g0089141 60.965 228 32 3 1 171 1 228 1.81e-86 253
Msa1110240 MtrunA17_Chr2g0325971 60.965 228 32 3 1 171 1 228 2.29e-85 250
Msa1110240 MtrunA17_Chr1g0208581 83.929 112 18 0 60 171 117 228 3.69e-66 201
Msa1110240 MtrunA17_Chr1g0208581 85.000 60 9 0 1 60 1 60 1.11e-28 105
Msa1110240 MtrunA17_Chr1g0187361 48.936 235 45 6 1 170 1 225 1.62e-60 187
Msa1110240 MtrunA17_Chr1g0188671 47.660 235 48 6 1 170 39 263 1.88e-56 178
Msa1110240 MtrunA17_Chr5g0419601 45.106 235 54 5 1 170 1 225 2.57e-54 172
Msa1110240 MtrunA17_Chr5g0422941 44.681 235 55 5 1 170 1 225 3.14e-52 166
Msa1110240 MtrunA17_Chr2g0292791 45.249 221 54 4 1 156 1 219 1.31e-48 157
Msa1110240 MtrunA17_Chr5g0422421 45.106 235 54 6 1 170 1 225 2.31e-48 156
Msa1110240 MtrunA17_Chr3g0099411 48.515 202 56 5 1 156 1 200 3.22e-47 153
Msa1110240 MtrunA17_Chr8g0355091 41.850 227 65 5 1 162 1 225 5.00e-42 140
Msa1110240 MtrunA17_Chr2g0317451 41.702 235 62 6 1 170 1 225 9.77e-41 137
Msa1110240 MtrunA17_Chr2g0317481 41.850 227 65 5 1 162 1 225 1.23e-40 137
Msa1110240 MtrunA17_Chr1g0206721 65.714 105 26 2 60 156 117 219 1.92e-37 129
Msa1110240 MtrunA17_Chr1g0206721 87.097 62 7 1 1 62 1 61 8.45e-30 108
Msa1110240 MtrunA17_Chr1g0206701 57.983 119 32 3 60 170 117 225 1.44e-36 126
Msa1110240 MtrunA17_Chr1g0206701 87.097 62 7 1 1 62 1 61 9.71e-30 108
Msa1110240 MtrunA17_Chr1g0149391 61.905 105 30 2 60 156 117 219 1.79e-35 123
Msa1110240 MtrunA17_Chr1g0149391 83.871 62 9 1 1 62 1 61 1.92e-29 108
Msa1110240 MtrunA17_Chr1g0206691 55.462 119 35 3 60 170 100 208 1.96e-34 120
Msa1110240 MtrunA17_Chr1g0206691 84.444 45 6 1 18 62 1 44 2.83e-18 78.6
Msa1110240 MtrunA17_Chr2g0300441 56.303 119 34 3 60 170 117 225 3.81e-33 117
Msa1110240 MtrunA17_Chr2g0300441 80.000 60 12 0 1 60 1 60 6.00e-25 96.7
Msa1110240 MtrunA17_Chr3g0099421 59.048 105 33 2 60 156 142 244 7.97e-32 115
Msa1110240 MtrunA17_Chr3g0099421 82.258 62 10 1 1 62 26 86 1.92e-28 106
Msa1110240 MtrunA17_Chr7g0238991 49.645 141 53 3 38 170 107 237 5.38e-31 112
Msa1110240 MtrunA17_Chr7g0238991 67.391 46 14 1 16 60 27 72 2.25e-11 60.5
Msa1110240 MtrunA17_Chr2g0300421 88.333 60 7 0 1 60 1 60 1.88e-30 108
Msa1110240 MtrunA17_Chr1g0206991 82.258 62 10 1 1 62 1 61 1.16e-28 104
Msa1110240 MtrunA17_Chr1g0206991 57.576 66 20 1 60 117 117 182 1.08e-15 70.9
Msa1110240 MtrunA17_Chr3g0089151 81.667 60 11 0 1 60 1 60 7.61e-27 98.6
Msa1110240 MtrunA17_Chr3g0111841 80.435 46 9 0 15 60 2 47 2.65e-19 77.4
Msa1110240 MtrunA17_Chr8g0379021 64.286 56 20 0 5 60 8 63 3.04e-19 80.1
Msa1110240 MtrunA17_Chr3g0085241 65.517 58 20 0 3 60 35 92 3.00e-18 79.0
Msa1110240 MtrunA17_Chr3g0119901 69.231 52 16 0 9 60 9 60 3.40e-18 78.2
Msa1110240 MtrunA17_Chr2g0333981 60.714 56 22 0 5 60 8 63 2.81e-16 72.4
Msa1110240 MtrunA17_Chr1g0187351 79.070 43 9 0 18 60 1 43 4.16e-16 69.3
Msa1110240 MtrunA17_Chr3g0097101 45.679 81 43 1 3 83 11 90 4.64e-15 70.5
Msa1110240 MtrunA17_Chr2g0284911 47.945 73 37 1 9 81 17 88 1.64e-14 69.3
Msa1110240 MtrunA17_Chr3g0077501 45.946 74 39 1 9 82 2 74 5.26e-14 67.4
Msa1110240 MtrunA17_Chr8g0380021 46.575 73 38 1 9 81 18 89 5.96e-14 67.4
Msa1110240 MtrunA17_Chr1g0173161 55.769 52 23 0 9 60 2 53 6.85e-14 63.9
Msa1110240 MtrunA17_Chr2g0308251 55.769 52 23 0 9 60 2 53 9.87e-14 63.5
Msa1110240 MtrunA17_Chr8g0376821 55.769 52 23 0 9 60 2 53 9.89e-14 65.9
Msa1110240 MtrunA17_Chr7g0246381 53.846 52 24 0 9 60 2 53 1.54e-13 62.4
Msa1110240 MtrunA17_Chr1g0169761 55.769 52 23 0 9 60 2 53 1.69e-13 65.1
Msa1110240 MtrunA17_Chr1g0175601 57.143 49 21 0 9 57 2 50 1.83e-13 65.1
Msa1110240 MtrunA17_Chr5g0425191 55.769 52 23 0 9 60 2 53 1.96e-13 63.2
Msa1110240 MtrunA17_Chr4g0049901 62.745 51 19 0 10 60 29 79 2.86e-13 65.5
Msa1110240 MtrunA17_Chr6g0474371 55.556 54 22 1 7 60 2 53 2.86e-13 64.7
Msa1110240 MtrunA17_Chr5g0412271 54.098 61 26 1 9 69 2 60 3.17e-13 65.1
Msa1110240 MtrunA17_Chr4g0057151 60.784 51 20 0 10 60 3 53 3.45e-13 64.7
Msa1110240 MtrunA17_Chr5g0412461 55.000 60 25 1 10 69 3 60 3.61e-13 65.1
Msa1110240 MtrunA17_Chr8g0377561 51.923 52 25 0 9 60 2 53 4.77e-13 62.0
Msa1110240 MtrunA17_Chr4g0044071 53.846 52 24 0 9 60 2 53 5.06e-13 62.4
Msa1110240 MtrunA17_Chr1g0159661 39.506 81 40 1 9 80 2 82 5.80e-13 63.5
Msa1110240 MtrunA17_Chr1g0180591 55.102 49 22 0 9 57 2 50 7.55e-13 63.5
Msa1110240 MtrunA17_Chr3g0134451 55.769 52 23 0 9 60 2 53 7.73e-13 65.1
Msa1110240 MtrunA17_Chr1g0164791 55.769 52 23 0 9 60 2 53 8.48e-13 63.9
Msa1110240 MtrunA17_Chr1g0175611 55.102 49 22 0 9 57 2 50 8.65e-13 63.2
Msa1110240 MtrunA17_Chr4g0018951 33.129 163 54 3 5 117 8 165 8.65e-13 63.2
Msa1110240 MtrunA17_Chr4g0074101 53.846 52 24 0 9 60 2 53 1.06e-12 63.2
Msa1110240 MtrunA17_Chr5g0427401 55.769 52 23 0 9 60 2 53 1.06e-12 63.9
Msa1110240 MtrunA17_Chr8g0357771 55.769 52 23 0 9 60 2 53 1.39e-12 62.8
Msa1110240 MtrunA17_Chr4g0020221 55.769 52 23 0 9 60 2 53 1.46e-12 63.9
Msa1110240 MtrunA17_Chr8g0351051 53.846 52 24 0 9 60 2 53 1.71e-12 63.5
Msa1110240 MtrunA17_Chr4g0061691 51.923 52 25 0 9 60 2 53 1.89e-12 63.2
Msa1110240 MtrunA17_Chr4g0061671 51.852 54 24 1 7 60 2 53 2.34e-12 62.0
Msa1110240 MtrunA17_Chr7g0220111 53.704 54 23 1 7 60 2 53 2.71e-12 62.8
Msa1110240 MtrunA17_Chr5g0420091 51.923 52 25 0 9 60 2 53 3.14e-12 62.4
Msa1110240 MtrunA17_Chr8g0366231 51.923 52 25 0 9 60 2 53 3.86e-12 62.4
Msa1110240 MtrunA17_Chr5g0420081 50.000 52 26 0 9 60 2 53 3.89e-12 62.4
Msa1110240 MtrunA17_Chr3g0122251 53.846 52 24 0 9 60 2 53 4.10e-12 62.4
Msa1110240 MtrunA17_Chr3g0111831 63.830 47 16 1 16 62 11 56 4.45e-12 58.9
Msa1110240 MtrunA17_Chr8g0351041 54.902 51 23 0 10 60 3 53 5.48e-12 60.8
Msa1110240 MtrunA17_Chr6g0455511 51.923 52 25 0 9 60 2 53 6.31e-12 62.0
Msa1110240 MtrunA17_Chr3g0125241 50.000 52 26 0 9 60 27 78 6.56e-12 61.2
Msa1110240 MtrunA17_Chr4g0052081 56.863 51 22 0 9 59 2 52 6.59e-12 60.8
Msa1110240 MtrunA17_Chr1g0204111 45.205 73 39 1 9 81 18 89 6.73e-12 62.0
Msa1110240 MtrunA17_Chr7g0246431 54.902 51 23 0 10 60 3 53 7.10e-12 61.2
Msa1110240 MtrunA17_Chr2g0279821 51.923 52 25 0 9 60 2 53 7.82e-12 61.6
Msa1110240 MtrunA17_Chr8g0351031 54.902 51 23 0 10 60 3 53 1.03e-11 61.2
Msa1110240 MtrunA17_Chr3g0142191 50.000 52 26 0 9 60 2 53 1.05e-11 61.2
Msa1110240 MtrunA17_Chr4g0060841 40.426 94 51 3 9 98 2 94 1.28e-11 61.6
Msa1110240 MtrunA17_Chr4g0052061 53.846 52 24 0 9 60 2 53 1.37e-11 59.7
Msa1110240 MtrunA17_Chr5g0427921 46.154 52 28 0 9 60 2 53 3.25e-11 56.2
Msa1110240 MtrunA17_Chr8g0356071 53.846 52 24 0 9 60 2 53 5.29e-11 58.2
Msa1110240 MtrunA17_Chr5g0404951 51.923 52 25 0 9 60 2 53 8.90e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1110240 AT5G60440.1 79.310 58 11 1 5 62 3 59 8.63e-24 95.1
Msa1110240 AT1G01530.1 73.684 57 15 0 4 60 2 58 2.06e-22 90.5
Msa1110240 AT4G36590.1 75.000 56 14 0 5 60 3 58 5.89e-22 89.4
Msa1110240 AT1G65360.1 66.071 56 19 0 5 60 3 58 3.41e-19 81.6
Msa1110240 AT2G24840.1 67.308 52 17 0 9 60 63 114 4.91e-17 76.6
Msa1110240 AT2G42830.1 45.349 86 43 3 2 87 12 93 2.06e-14 68.9
Msa1110240 AT2G42830.2 45.349 86 43 3 2 87 12 93 2.27e-14 68.9
Msa1110240 AT3G58780.1 45.349 86 43 3 2 87 12 93 3.12e-14 68.6
Msa1110240 AT3G58780.4 45.349 86 43 3 2 87 12 93 3.24e-14 68.9
Msa1110240 AT3G58780.5 45.349 86 43 3 2 87 18 99 3.34e-14 68.6
Msa1110240 AT4G09960.1 45.570 79 41 2 9 87 2 78 4.22e-14 68.2
Msa1110240 AT4G09960.6 45.570 79 41 2 9 87 3 79 4.24e-14 68.2
Msa1110240 AT4G09960.2 45.570 79 41 2 9 87 2 78 4.49e-14 67.8
Msa1110240 AT4G09960.5 45.570 79 41 2 9 87 12 88 4.85e-14 68.2
Msa1110240 AT3G61120.1 40.476 84 48 1 9 92 2 83 6.31e-14 67.8
Msa1110240 AT4G09960.3 45.570 79 41 2 9 87 28 104 6.34e-14 67.8
Msa1110240 AT2G34440.1 59.615 52 21 0 9 60 2 53 6.73e-14 66.2
Msa1110240 AT1G72350.1 29.870 154 77 3 9 131 43 196 1.05e-13 67.0
Msa1110240 AT2G22540.2 56.863 51 22 0 10 60 3 53 1.79e-13 66.2
Msa1110240 AT2G22540.3 55.769 52 23 0 9 60 2 53 2.58e-13 65.9
Msa1110240 AT2G22540.1 55.769 52 23 0 9 60 2 53 2.58e-13 65.9
Msa1110240 AT3G04100.1 41.558 77 35 1 2 68 7 83 2.81e-13 65.5
Msa1110240 AT3G66656.1 59.615 52 21 0 9 60 2 53 3.20e-13 64.7
Msa1110240 AT5G51870.2 60.784 51 20 0 10 60 3 53 4.22e-13 64.3
Msa1110240 AT5G13790.1 59.615 52 21 0 9 60 2 53 4.35e-13 65.9
Msa1110240 AT5G13790.2 59.615 52 21 0 9 60 2 53 4.65e-13 65.9
Msa1110240 AT3G58780.2 55.932 59 24 1 2 60 12 68 5.26e-13 65.1
Msa1110240 AT3G58780.3 55.932 59 24 1 2 60 12 68 5.60e-13 65.5
Msa1110240 AT5G20240.2 41.975 81 38 1 9 80 2 82 6.60e-13 63.2
Msa1110240 AT4G09960.4 45.570 79 41 2 9 87 97 173 7.66e-13 65.5
Msa1110240 AT5G23260.1 55.357 56 25 0 5 60 14 69 8.65e-13 64.7
Msa1110240 AT5G20240.1 41.975 81 38 1 9 80 2 82 8.66e-13 63.9
Msa1110240 AT5G23260.4 55.357 56 25 0 5 60 14 69 8.68e-13 65.1
Msa1110240 AT5G51870.5 57.143 56 24 0 5 60 21 76 1.03e-12 63.5
Msa1110240 AT4G24540.1 55.769 52 23 0 9 60 2 53 1.04e-12 63.9
Msa1110240 AT5G51870.1 60.784 51 20 0 10 60 3 53 1.09e-12 63.9
Msa1110240 AT2G45650.1 55.769 52 23 0 9 60 2 53 1.14e-12 64.3
Msa1110240 AT5G23260.3 57.692 52 22 0 9 60 2 53 1.40e-12 63.9
Msa1110240 AT1G22130.1 55.769 52 23 0 9 60 2 53 1.45e-12 64.7
Msa1110240 AT5G23260.2 57.692 52 22 0 9 60 2 53 1.47e-12 63.9
Msa1110240 AT4G11880.2 56.863 51 22 0 10 60 3 53 1.56e-12 62.8
Msa1110240 AT4G11880.3 56.863 51 22 0 10 60 3 53 1.56e-12 62.8
Msa1110240 AT4G18960.1 55.769 52 23 0 9 60 18 69 1.61e-12 63.9
Msa1110240 AT3G57390.3 55.769 52 23 0 9 60 2 53 1.76e-12 63.5
Msa1110240 AT1G77980.1 55.769 52 23 0 9 60 2 53 1.85e-12 64.3
Msa1110240 AT5G51870.4 60.784 51 20 0 10 60 3 53 1.91e-12 63.2
Msa1110240 AT5G51870.3 60.784 51 20 0 10 60 13 63 1.94e-12 63.5
Msa1110240 AT1G69120.2 55.556 54 22 1 7 60 2 53 2.51e-12 63.2
Msa1110240 AT4G11880.4 56.863 51 22 0 10 60 3 53 2.61e-12 63.2
Msa1110240 AT4G11880.1 56.863 51 22 0 10 60 3 53 2.61e-12 63.2
Msa1110240 AT2G03710.3 53.846 52 24 0 9 60 2 53 2.92e-12 62.4
Msa1110240 AT1G26310.1 53.704 54 23 1 7 60 2 53 3.24e-12 63.2
Msa1110240 AT5G60910.1 53.846 52 24 0 9 60 2 53 3.42e-12 63.2
Msa1110240 AT3G57390.1 55.769 52 23 0 9 60 2 53 3.58e-12 63.2
Msa1110240 AT5G10140.2 53.846 52 24 0 9 60 2 53 3.69e-12 61.6
Msa1110240 AT1G24260.3 31.176 170 99 5 9 163 2 168 4.35e-12 62.8
Msa1110240 AT1G69120.1 53.846 52 24 0 9 60 2 53 4.42e-12 62.8
Msa1110240 AT5G10140.4 53.846 52 24 0 9 60 2 53 4.62e-12 61.6
Msa1110240 AT5G10140.1 53.846 52 24 0 9 60 2 53 5.33e-12 61.6
Msa1110240 AT5G15800.1 51.923 52 25 0 9 60 2 53 6.00e-12 62.4
Msa1110240 AT1G24260.2 51.923 52 25 0 9 60 2 53 7.71e-12 62.0
Msa1110240 AT5G10140.3 53.846 52 24 0 9 60 2 53 7.91e-12 61.2
Msa1110240 AT3G30260.1 50.000 52 26 0 9 60 2 53 8.20e-12 62.0
Msa1110240 AT1G24260.1 51.923 52 25 0 9 60 2 53 8.45e-12 62.0
Msa1110240 AT2G03710.2 53.846 52 24 0 9 60 2 53 8.55e-12 62.0
Msa1110240 AT4G22950.2 56.863 51 22 0 10 60 3 53 8.85e-12 60.8
Msa1110240 AT5G15800.2 51.923 52 25 0 9 60 2 53 9.10e-12 62.0
Msa1110240 AT2G03710.1 53.846 52 24 0 9 60 2 53 9.16e-12 62.0
Msa1110240 AT5G62165.6 54.902 51 23 0 10 60 3 53 9.43e-12 60.1
Msa1110240 AT5G62165.9 54.902 51 23 0 10 60 3 53 9.43e-12 60.1
Msa1110240 AT5G62165.7 54.902 51 23 0 10 60 3 53 9.43e-12 60.1
Msa1110240 AT5G62165.8 54.902 51 23 0 10 60 3 53 9.43e-12 60.1
Msa1110240 AT5G62165.13 54.902 51 23 0 10 60 3 53 9.43e-12 60.1
Msa1110240 AT5G62165.14 54.902 51 23 0 10 60 3 53 1.03e-11 58.9
Msa1110240 AT3G02310.2 51.923 52 25 0 9 60 2 53 1.10e-11 60.8
Msa1110240 AT3G54340.1 34.579 107 58 2 9 107 2 104 1.23e-11 61.2
Msa1110240 AT3G02310.1 51.923 52 25 0 9 60 2 53 1.29e-11 61.6
Msa1110240 AT3G57230.2 48.077 52 27 0 9 60 2 53 1.29e-11 59.7
Msa1110240 AT2G45660.2 52.941 51 24 0 10 60 3 53 1.33e-11 60.1
Msa1110240 AT5G62165.17 54.902 51 23 0 10 60 3 53 1.45e-11 59.7
Msa1110240 AT5G62165.12 54.902 51 23 0 10 60 3 53 1.45e-11 59.7
Msa1110240 AT5G62165.11 54.902 51 23 0 10 60 3 53 1.45e-11 59.7
Msa1110240 AT4G22950.3 56.863 51 22 0 10 60 3 53 1.59e-11 60.8
Msa1110240 AT4G22950.1 56.863 51 22 0 10 60 3 53 1.59e-11 60.8
Msa1110240 AT5G51860.2 49.231 65 33 0 10 74 3 67 2.33e-11 60.1
Msa1110240 AT3G57230.1 48.077 52 27 0 9 60 2 53 2.54e-11 60.5
Msa1110240 AT3G57230.4 48.077 52 27 0 9 60 2 53 2.54e-11 60.5
Msa1110240 AT5G51860.1 49.231 65 33 0 10 74 3 67 2.65e-11 60.1
Msa1110240 AT2G14210.1 50.000 52 26 0 9 60 2 53 2.72e-11 60.5
Msa1110240 AT1G77950.3 51.923 52 25 0 9 60 2 53 2.74e-11 60.5
Msa1110240 AT1G77950.2 51.923 52 25 0 9 60 2 53 2.74e-11 60.5
Msa1110240 AT1G77950.1 51.923 52 25 0 9 60 2 53 2.74e-11 60.5
Msa1110240 AT1G77950.4 51.923 52 25 0 9 60 2 53 2.74e-11 60.5
Msa1110240 AT5G62165.10 54.902 51 23 0 10 60 3 53 3.20e-11 59.7
Msa1110240 AT5G62165.4 54.902 51 23 0 10 60 3 53 3.20e-11 59.7
Msa1110240 AT2G14210.2 50.000 52 26 0 9 60 2 53 3.30e-11 60.1
Msa1110240 AT5G62165.3 54.902 51 23 0 10 60 3 53 3.70e-11 59.7
Msa1110240 AT5G62165.1 54.902 51 23 0 10 60 3 53 3.70e-11 59.7
Msa1110240 AT5G62165.2 54.902 51 23 0 10 60 3 53 3.70e-11 59.7
Msa1110240 AT2G45660.1 52.941 51 24 0 10 60 3 53 3.77e-11 59.7
Msa1110240 AT5G55690.1 43.103 58 31 1 9 66 2 57 7.49e-11 59.7
Msa1110240 AT5G55690.2 43.103 58 31 1 9 66 2 57 7.49e-11 59.7

Find 34 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAAATCTGAGAAGAAAAT+TGG 0.264401 7_3:+54933691 Msa1110240:intron
TTGCAGCCTCATTTGTTTGA+TGG 0.293834 7_3:+54933979 Msa1110240:CDS
TGGTGGGCTTGTCCGGTTGA+TGG 0.317499 7_3:+54933757 Msa1110240:CDS
ATTTCAGCTTGTTGAGCATT+TGG 0.333852 7_3:-54933919 None:intergenic
TCACCACGGCTTCAATAATA+TGG 0.342966 7_3:+54934014 Msa1110240:CDS
TGAATTATTCAAGAAAGCTT+TGG 0.360061 7_3:+54933798 Msa1110240:CDS
GCTTTGAGAAAGTCACTTGC+AGG 0.373101 7_3:-54933438 None:intergenic
TCCGGTTGATGGGATGAATA+GGG 0.429925 7_3:+54933768 Msa1110240:CDS
GTCCGGTTGATGGGATGAAT+AGG 0.433236 7_3:+54933767 Msa1110240:CDS
AGTGAGCTTTGCACCCTTTG+TGG 0.435393 7_3:+54933487 Msa1110240:CDS
GGTGGGCTTGTCCGGTTGAT+GGG 0.441656 7_3:+54933758 Msa1110240:CDS
AGGCAACGAAGGCTAAGTTT+TGG 0.466330 7_3:+54933737 Msa1110240:CDS
TCCCTATTCATCCCATCAAC+CGG 0.481029 7_3:-54933769 None:intergenic
AGCAGTATCTGCACCACAAA+GGG 0.482731 7_3:-54933500 None:intergenic
ATTATTCAAGAAAGCTTTGG+AGG 0.484545 7_3:+54933801 Msa1110240:CDS
TGAGGCTGCAATTGCAACAT+AGG 0.486430 7_3:-54933967 None:intergenic
GCCTTTCTTTCCGCTCGACA+TGG 0.505646 7_3:-54933368 None:intergenic
ATGATTGAGATATATGTTTG+AGG 0.515672 7_3:-54933893 None:intergenic
ACAAAGGGTGCAAAGCTCAC+TGG 0.517842 7_3:-54933485 None:intergenic
GCCATGTCGAGCGGAAAGAA+AGG 0.520307 7_3:+54933367 None:intergenic
TCTCGATCTTCTGGCGACCT+CGG 0.542422 7_3:-54933390 None:intergenic
CAATTTGCGCAAGGCAACGA+AGG 0.559033 7_3:+54933726 Msa1110240:CDS
ATTGATCCATCAAACAAATG+AGG 0.563188 7_3:-54933985 None:intergenic
GAGGCTGCAATTGCAACATA+GGG 0.578323 7_3:-54933966 None:intergenic
CTCGTTGTATTCTCACCTAG+TGG 0.580084 7_3:+54933523 Msa1110240:CDS
GCACCATATTATTGAAGCCG+TGG 0.584289 7_3:-54934017 None:intergenic
TCGAGCGGAAAGAAAGGCCG+AGG 0.597603 7_3:+54933373 Msa1110240:CDS
TGTATTCTCACCTAGTGGAA+AGG 0.611194 7_3:+54933528 Msa1110240:CDS
TGAGTTGAGCAATTTGCGCA+AGG 0.614191 7_3:+54933717 Msa1110240:CDS
ATATTATTGAAGCCGTGGTG+AGG 0.616210 7_3:-54934012 None:intergenic
AAGGAAAATACCTTTCCACT+AGG 0.624124 7_3:-54933538 None:intergenic
GAGCAGTATCTGCACCACAA+AGG 0.630236 7_3:-54933501 None:intergenic
TGTGCGTCAGCCATGTCGAG+CGG 0.650334 7_3:+54933358 None:intergenic
GGATCAATGATGCCTCACCA+CGG 0.697568 7_3:+54934000 Msa1110240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGAATTATTCAAGAAAGCTT+TGG + chr7_3:54933798-54933817 Msa1110240:CDS 25.0%
! ATGATTGAGATATATGTTTG+AGG - chr7_3:54933896-54933915 None:intergenic 25.0%
!!! ATAGGGTTTTGAATAAATTG+TGG - chr7_3:54933952-54933971 None:intergenic 25.0%
CTCAAATCTGAGAAGAAAAT+TGG + chr7_3:54933691-54933710 Msa1110240:intron 30.0%
! ATTATTCAAGAAAGCTTTGG+AGG + chr7_3:54933801-54933820 Msa1110240:CDS 30.0%
ATTGATCCATCAAACAAATG+AGG - chr7_3:54933988-54934007 None:intergenic 30.0%
!! TCTTTTTCATCTCGATCTTC+TGG - chr7_3:54933402-54933421 None:intergenic 35.0%
AAGGAAAATACCTTTCCACT+AGG - chr7_3:54933541-54933560 None:intergenic 35.0%
!!! AGTGGAAAGGTATTTTCCTT+TGG + chr7_3:54933541-54933560 Msa1110240:intron 35.0%
TCTATGACCGTATCAAGATT+GGG - chr7_3:54933571-54933590 None:intergenic 35.0%
! ATTGCATAGTGCCATTGTTT+GGG - chr7_3:54933620-54933639 None:intergenic 35.0%
! AATTGCATAGTGCCATTGTT+TGG - chr7_3:54933621-54933640 None:intergenic 35.0%
!! GAAGCGTTGTCAACAAAAAA+TGG - chr7_3:54933871-54933890 None:intergenic 35.0%
!! ATTTCAGCTTGTTGAGCATT+TGG - chr7_3:54933922-54933941 None:intergenic 35.0%
!!! GGGTTTTGAATAAATTGTGG+TGG - chr7_3:54933949-54933968 None:intergenic 35.0%
TGTATTCTCACCTAGTGGAA+AGG + chr7_3:54933528-54933547 Msa1110240:CDS 40.0%
CTATGACCGTATCAAGATTG+GGG - chr7_3:54933570-54933589 None:intergenic 40.0%
GTCTATGACCGTATCAAGAT+TGG - chr7_3:54933572-54933591 None:intergenic 40.0%
TGGCACTATGCAATTCATTG+AGG + chr7_3:54933626-54933645 Msa1110240:intron 40.0%
!! AACGAAGGCTAAGTTTTGGT+GGG + chr7_3:54933741-54933760 Msa1110240:CDS 40.0%
TTGCAGCCTCATTTGTTTGA+TGG + chr7_3:54933979-54933998 Msa1110240:CDS 40.0%
ATATTATTGAAGCCGTGGTG+AGG - chr7_3:54934015-54934034 None:intergenic 40.0%
TCACCACGGCTTCAATAATA+TGG + chr7_3:54934014-54934033 Msa1110240:CDS 40.0%
!! GCTTTGAGAAAGTCACTTGC+AGG - chr7_3:54933441-54933460 None:intergenic 45.0%
AGCAGTATCTGCACCACAAA+GGG - chr7_3:54933503-54933522 None:intergenic 45.0%
CTCGTTGTATTCTCACCTAG+TGG + chr7_3:54933523-54933542 Msa1110240:CDS 45.0%
ATCAAGATTGGGGTGACCAA+AGG - chr7_3:54933560-54933579 None:intergenic 45.0%
TGGTCACCCCAATCTTGATA+CGG + chr7_3:54933561-54933580 Msa1110240:intron 45.0%
GTGAGACGAGGGAAAGATAA+CGG - chr7_3:54933594-54933613 None:intergenic 45.0%
! CATAGTGCCATTGTTTGGGA+GGG - chr7_3:54933616-54933635 None:intergenic 45.0%
TTCATTGAGGCTAACCGCAA+TGG + chr7_3:54933639-54933658 Msa1110240:intron 45.0%
GTTCATGCACGTTTCCATTG+CGG - chr7_3:54933656-54933675 None:intergenic 45.0%
! TGAGTTGAGCAATTTGCGCA+AGG + chr7_3:54933717-54933736 Msa1110240:CDS 45.0%
! AGGCAACGAAGGCTAAGTTT+TGG + chr7_3:54933737-54933756 Msa1110240:CDS 45.0%
!! CAACGAAGGCTAAGTTTTGG+TGG + chr7_3:54933740-54933759 Msa1110240:CDS 45.0%
!!! TAAGTTTTGGTGGGCTTGTC+CGG + chr7_3:54933750-54933769 Msa1110240:CDS 45.0%
!! TCCGGTTGATGGGATGAATA+GGG + chr7_3:54933768-54933787 Msa1110240:CDS 45.0%
TCCCTATTCATCCCATCAAC+CGG - chr7_3:54933772-54933791 None:intergenic 45.0%
GAGGCTGCAATTGCAACATA+GGG - chr7_3:54933969-54933988 None:intergenic 45.0%
TGAGGCTGCAATTGCAACAT+AGG - chr7_3:54933970-54933989 None:intergenic 45.0%
GCACCATATTATTGAAGCCG+TGG - chr7_3:54934020-54934039 None:intergenic 45.0%
! ACAAAGGGTGCAAAGCTCAC+TGG - chr7_3:54933488-54933507 None:intergenic 50.0%
AGTGAGCTTTGCACCCTTTG+TGG + chr7_3:54933487-54933506 Msa1110240:CDS 50.0%
GAGCAGTATCTGCACCACAA+AGG - chr7_3:54933504-54933523 None:intergenic 50.0%
! GCATAGTGCCATTGTTTGGG+AGG - chr7_3:54933617-54933636 None:intergenic 50.0%
CAATTTGCGCAAGGCAACGA+AGG + chr7_3:54933726-54933745 Msa1110240:CDS 50.0%
!! GTCCGGTTGATGGGATGAAT+AGG + chr7_3:54933767-54933786 Msa1110240:CDS 50.0%
GGATCAATGATGCCTCACCA+CGG + chr7_3:54934000-54934019 Msa1110240:CDS 50.0%
TCTCGATCTTCTGGCGACCT+CGG - chr7_3:54933393-54933412 None:intergenic 55.0%
TGTTTGGGAGGGTGAGACGA+GGG - chr7_3:54933605-54933624 None:intergenic 55.0%
TTGTTTGGGAGGGTGAGACG+AGG - chr7_3:54933606-54933625 None:intergenic 55.0%
CGTCTCACCCTCCCAAACAA+TGG + chr7_3:54933606-54933625 Msa1110240:intron 55.0%
TCGAGCGGAAAGAAAGGCCG+AGG + chr7_3:54933373-54933392 Msa1110240:CDS 60.0%
!! TGGTGGGCTTGTCCGGTTGA+TGG + chr7_3:54933757-54933776 Msa1110240:CDS 60.0%
! GGTGGGCTTGTCCGGTTGAT+GGG + chr7_3:54933758-54933777 Msa1110240:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 54933370 54934044 54933370 ID=Msa1110240;Name=Msa1110240
chr7_3 mRNA 54933370 54934044 54933370 ID=Msa1110240-mRNA-1;Parent=Msa1110240;Name=Msa1110240-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|0|0|1|1|1|2|0|172
chr7_3 exon 54933370 54933549 54933370 ID=Msa1110240-mRNA-1:exon:11564;Parent=Msa1110240-mRNA-1
chr7_3 exon 54933706 54934044 54933706 ID=Msa1110240-mRNA-1:exon:11565;Parent=Msa1110240-mRNA-1
chr7_3 CDS 54933370 54933549 54933370 ID=Msa1110240-mRNA-1:cds;Parent=Msa1110240-mRNA-1
chr7_3 CDS 54933706 54934044 54933706 ID=Msa1110240-mRNA-1:cds;Parent=Msa1110240-mRNA-1
Gene Sequence

>Msa1110240

ATGTCGAGCGGAAAGAAAGGCCGAGGTCGCCAGAAGATCGAGATGAAAAAGATGAGCAACGAGAGCAACCTGCAAGTGACTTTCTCAAAGCGTCGCAGCAGACTCTTCAAGAAATCCAGTGAGCTTTGCACCCTTTGTGGTGCAGATACTGCTCTCGTTGTATTCTCACCTAGTGGAAAGAAAATTGGTGATGAGTTGAGCAATTTGCGCAAGGCAACGAAGGCTAAGTTTTGGTGGGCTTGTCCGGTTGATGGGATGAATAGGGATCAACTTGAATTATTCAAGAAAGCTTTGGAGGAGCATAAGAAGCTTGTAATTCAGCATGCTGCAACTCGAACTCTTCCATTTTTTGTTGACAACGCTTCTTCCTCAAACATATATCTCAATCATCAGCCAAATGCTCAACAAGCTGAAATGTTGCCACCACAATTTATTCAAAACCCTATGTTGCAATTGCAGCCTCATTTGTTTGATGGATCAATGATGCCTCACCACGGCTTCAATAATATGGTGCAATAA
Protein sequence

>Msa1110240

MSSGKKGRGRQKIEMKKMSNESNLQVTFSKRRSRLFKKSSELCTLCGADTALVVFSPSGKKIGDELSNLRKATKAKFWWACPVDGMNRDQLELFKKALEEHKKLVIQHAATRTLPFFVDNASSSNIYLNHQPNAQQAEMLPPQFIQNPMLQLQPHLFDGSMMPHHGFNNMVQ*