AlfalfaGEDB | Msa1110340

M. sativa cultivar zhongmu-4 / Msa1110340

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1110340	AES79839.1	94.659	337	17	1	1	336	17	353	0.0	664

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1110340	sp\|Q5CCK4\|VAL2_ARATH	45.373	335	146	7	24	325	203	533	4.66e-82	268

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1110340	G7KY51	94.659	337	17	1	1	336	17	353	0.0	664

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TFs/TRs:

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Gene ID	Type	Classification
Msa1110340	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1110340	MtrunA17_Chr2g0281191	43.871	310	138	5	73	346	320	629	2.64e-73	244
Msa1110340	MtrunA17_Chr4g0012861	53.302	212	96	2	1	212	213	421	8.43e-61	209
Msa1110340	MtrunA17_Chr4g0012861	51.471	136	52	3	214	336	484	618	1.38e-36	140
Msa1110340	MtrunA17_Chr7g0244881	92.784	97	7	0	1	97	17	113	1.15e-60	191
Msa1110340	MtrunA17_Chr7g0244871	94.681	94	5	0	97	190	4	97	2.22e-60	189
Msa1110340	MtrunA17_Chr3g0107701	46.781	233	111	8	1	225	233	460	3.47e-54	191
Msa1110340	MtrunA17_Chr3g0107701	49.587	121	48	2	229	336	522	642	2.49e-28	116
Msa1110340	MtrunA17_Chr5g0432721	48.649	185	90	3	36	219	254	434	1.05e-50	181
Msa1110340	MtrunA17_Chr5g0432721	55.556	72	31	1	231	301	507	578	1.18e-17	84.7
Msa1110340	MtrunA17_Chr7g0251891	38.835	103	62	1	105	206	16	118	5.31e-18	81.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1110340	AT4G32010.2	45.921	331	146	6	24	325	203	529	6.09e-85	273
Msa1110340	AT4G32010.1	45.373	335	146	7	24	325	203	533	4.74e-83	268
Msa1110340	AT2G30470.1	42.059	340	148	6	34	325	218	556	1.21e-72	241
Msa1110340	AT4G21550.3	38.849	278	149	7	38	299	254	526	1.72e-48	172
Msa1110340	AT4G21550.1	44.053	227	116	4	84	301	304	528	2.50e-43	160
Msa1110340	AT4G21550.2	37.879	264	143	7	38	285	254	512	1.48e-42	155
Msa1110340	AT3G26790.1	40.385	104	61	1	105	207	91	194	4.10e-18	84.3
Msa1110340	AT1G28300.1	39.669	121	69	3	72	189	136	255	7.19e-17	81.3
Msa1110340	AT3G24650.1	42.857	98	54	2	105	200	571	668	1.47e-16	81.6
Msa1110340	AT5G06250.4	39.695	131	60	5	105	218	45	173	5.78e-15	74.7

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Find 75 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATTTAACCGGATGGACC+CGG	0.514859	7_3:-55148064	Msa1110340:CDS
AACTGATAATATGGTTTCGA+AGG	0.505578	7_3:-55148035	Msa1110340:CDS
AAGAAGGGTATATTCATAAA+TGG	0.214466	7_3:-55147232	Msa1110340:CDS
AAGGAATTTGAATTTCCAAA+AGG	0.480284	7_3:+55149137	None:intergenic
AATAACATTGCTATGTCTAA+AGG	0.470824	7_3:-55147756	Msa1110340:CDS
AATATGGTTTCGAAGGGCAT+CGG	0.550897	7_3:-55148028	Msa1110340:CDS
AATCGCCTGTAAGACGACAA+AGG	0.682009	7_3:-55148950	Msa1110340:CDS
AATTCAAATTCCTTTGGTGG+TGG	0.581836	7_3:-55149128	Msa1110340:CDS
AATTCTTGATCGGTAATCTT+AGG	0.277029	7_3:+55148903	None:intergenic
AATTTGCAGTAACATTTAAC+CGG	0.402045	7_3:-55148074	Msa1110340:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTCTTGATCGGTAATCTT+AGG	+	chr7_3:55146640-55146659	None:intergenic	30.0%
GTTTGTATGATTAGTACTCT+TGG	-	chr7_3:55146885-55146904	Msa1110340:intron	30.0%
ATTTGTAAATAAATACCCCC+TGG	+	chr7_3:55147172-55147191	None:intergenic	30.0%
TATATCCTGCTGCTATTATT+TGG	+	chr7_3:55147241-55147260	None:intergenic	30.0%
AACTGATAATATGGTTTCGA+AGG	-	chr7_3:55147505-55147524	Msa1110340:intron	30.0%
ACTGATAATATGGTTTCGAA+GGG	-	chr7_3:55147506-55147525	Msa1110340:intron	30.0%
ATAGGAAGTTTCATTTCAGT+TGG	+	chr7_3:55147729-55147748	None:intergenic	30.0%
ATTGATGCTGAAACTTACTT+GGG	-	chr7_3:55147918-55147937	Msa1110340:intron	30.0%
ACAATGTTTGAATTAACCGT+TGG	+	chr7_3:55147973-55147992	None:intergenic	30.0%
TTCAAACATTGTAGTGATTG+AGG	-	chr7_3:55147981-55148000	Msa1110340:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	55146388	55149174	55146388	ID=Msa1110340;Name=Msa1110340
chr7_3	mRNA	55146388	55149174	55146388	ID=Msa1110340-mRNA-1;Parent=Msa1110340;Name=Msa1110340-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0\|0\|0\|0.87\|1\|1\|8\|0\|363
chr7_3	exon	55148885	55149174	55148885	ID=Msa1110340-mRNA-1:exon:11615;Parent=Msa1110340-mRNA-1
chr7_3	exon	55148518	55148614	55148518	ID=Msa1110340-mRNA-1:exon:11614;Parent=Msa1110340-mRNA-1
chr7_3	exon	55148243	55148417	55148243	ID=Msa1110340-mRNA-1:exon:11613;Parent=Msa1110340-mRNA-1
chr7_3	exon	55148014	55148087	55148014	ID=Msa1110340-mRNA-1:exon:11612;Parent=Msa1110340-mRNA-1
chr7_3	exon	55147536	55147781	55147536	ID=Msa1110340-mRNA-1:exon:11611;Parent=Msa1110340-mRNA-1
chr7_3	exon	55147232	55147272	55147232	ID=Msa1110340-mRNA-1:exon:11610;Parent=Msa1110340-mRNA-1
chr7_3	exon	55146813	55146896	55146813	ID=Msa1110340-mRNA-1:exon:11609;Parent=Msa1110340-mRNA-1
chr7_3	exon	55146388	55146472	55146388	ID=Msa1110340-mRNA-1:exon:11608;Parent=Msa1110340-mRNA-1
chr7_3	CDS	55148885	55149174	55148885	ID=Msa1110340-mRNA-1:cds;Parent=Msa1110340-mRNA-1
chr7_3	CDS	55148518	55148614	55148518	ID=Msa1110340-mRNA-1:cds;Parent=Msa1110340-mRNA-1
chr7_3	CDS	55148243	55148417	55148243	ID=Msa1110340-mRNA-1:cds;Parent=Msa1110340-mRNA-1
chr7_3	CDS	55148014	55148087	55148014	ID=Msa1110340-mRNA-1:cds;Parent=Msa1110340-mRNA-1
chr7_3	CDS	55147536	55147781	55147536	ID=Msa1110340-mRNA-1:cds;Parent=Msa1110340-mRNA-1
chr7_3	CDS	55147232	55147272	55147232	ID=Msa1110340-mRNA-1:cds;Parent=Msa1110340-mRNA-1
chr7_3	CDS	55146813	55146896	55146813	ID=Msa1110340-mRNA-1:cds;Parent=Msa1110340-mRNA-1
chr7_3	CDS	55146388	55146472	55146388	ID=Msa1110340-mRNA-1:cds;Parent=Msa1110340-mRNA-1

Gene Sequence

>Msa1110340

ATGACCTTTAATACTCCTTTTGGAAATTCAAATTCCTTTGGTGGTGGTGAAAAGCAACAACAAACTGAAAATTCAATTCCAAATCTCTCAGAGGGAGAGCCTAAGTCTCAGAATCTTTTGCACAAGCTTCCCAAGTCAACTCTTGCTATTGAGTCAGAGAAAAATGTGTGCATGGCATCTCCAATTCATCATGTCGCAAGGAAATCGCCTGTAAGACGACAAAGGAAACACATATCTGCACGTAATTTGCCTAAGATTACCGATCAAGAATTACAACAATTATTTGGAGACTTAAATTCCGCCATTGTGCCACTATTCGAGAAGACATTAACTGCTAGTGATGTTGGTCGACTTGGTCGCATGGTTCTTCCAAAATCATGTGTTGAAACATATTTTCCACCTATTTCGGAACCAGGGGGTATTTATTTACAAATCGAAGACGTGAAAGGGAAGAAATTGGTGTTTAAATTTAGATTTTGGCCAAATAATAGCAGCAGGATATATGTTTTAGAAGGAGTACATGCTTGGATTCAGTCTATGCAATTGCAAGTTGGAGATTTTGTAACATTTAACCGGATGGACCCGGGGGAGAAACTGATAATATGGTTTCGAAGGGCATCGGTTTCTTCTCTACAGGTGAATAACATTGCTATGTCTAAAGGCATGAAAAGAAAGAGAATGTGTTTGCCACAAGAGCTAGTAATTCCTAAGAAGAAAAGAACATGTGACATTGTGTCAAAAAGTAAGAGCTTGCTTATTGATAACCAAGAAGCATTGATGCTGAAACTTACTTGGGAAGAGGCTCAAGATTTTCTTCATCCACCTCCAACGGTTAATTCAAACATTGTAGTGATTGAGGATCATGTCTTTGAAGAATATGAAGAACCTCCTATCATTGCAAAGAAGGGTATATTCATAAATGGGATGAACGAGCAATGGATACAGTGTGAGAAGTGCTCAGAATGGAGAAAGTTGGAATCTGTGGAGAATTTATGGGGTCAAAGAAGATTCTTTGAAACCATTAAAAAGACTTTGACCTATATGATCTATGCAAAGAACTGGTTTTTGATAGAAGACAGTGATATCAATTGA

Protein sequence

>Msa1110340

MTFNTPFGNSNSFGGGEKQQQTENSIPNLSEGEPKSQNLLHKLPKSTLAIESEKNVCMASPIHHVARKSPVRRQRKHISARNLPKITDQELQQLFGDLNSAIVPLFEKTLTASDVGRLGRMVLPKSCVETYFPPISEPGGIYLQIEDVKGKKLVFKFRFWPNNSSRIYVLEGVHAWIQSMQLQVGDFVTFNRMDPGEKLIIWFRRASVSSLQVNNIAMSKGMKRKRMCLPQELVIPKKKRTCDIVSKSKSLLIDNQEALMLKLTWEEAQDFLHPPPTVNSNIVVIEDHVFEEYEEPPIIAKKGIFINGMNEQWIQCEKCSEWRKLESVENLWGQRRFFETIKKTLTYMIYAKNWFLIEDSDIN*