AlfalfaGEDB | Msa1117050

M. sativa cultivar zhongmu-4 / Msa1117050

KEGG: K14328
GO: GO:0000184 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0031974 GO:0031981 GO:0032870 GO:0034641 GO:0034655 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046677 GO:0046700 GO:0048519 GO:0048571 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1117050	XP_003598025.2	48.936	282	114	5	399	651	255	535	3.21e-65	239
Msa1117050	XP_003598025.2	51.119	268	101	5	3	241	269	535	7.64e-65	238
Msa1117050	XP_003598025.2	55.629	151	56	4	259	400	35	183	1.02e-36	158
Msa1117050	XP_003598025.2	58.278	151	57	3	669	815	35	183	4.31e-41	171
Msa1117050	XP_003598025.2	84.466	412	56	4	401	804	8	419	0.0	708
Msa1117050	XP_003598025.2	84.500	400	51	4	1	389	20	419	0.0	688

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1117050	sp\|Q8L3W1\|VRN1_ARATH	45.455	99	54	0	674	772	6	104	5.86e-21	98.6
Msa1117050	sp\|Q8L3W1\|VRN1_ARATH	44.681	94	52	0	264	357	6	99	1.26e-19	94.7
Msa1117050	sp\|Q8L3W1\|VRN1_ARATH	38.384	99	57	1	21	115	6	104	6.90e-15	80.5
Msa1117050	sp\|Q8L3W1\|VRN1_ARATH	38.384	99	57	1	431	525	6	104	6.90e-15	80.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1117050	G7IYE4	48.936	282	114	5	399	651	255	535	1.53e-65	239
Msa1117050	G7IYE4	51.119	268	101	5	3	241	269	535	3.65e-65	238
Msa1117050	G7IYE4	55.629	151	56	4	259	400	35	183	4.89e-37	158
Msa1117050	G7IYE4	58.278	151	57	3	669	815	35	183	2.06e-41	171
Msa1117050	G7IYE4	84.466	412	56	4	401	804	8	419	0.0	708
Msa1117050	G7IYE4	84.500	400	51	4	1	389	20	419	0.0	688

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TFs/TRs:

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Gene ID	Type	Classification
Msa1117050	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1117050	MtrunA17_Chr3g0077361	76.699	412	53	5	401	804	8	384	0.0	624
Msa1117050	MtrunA17_Chr3g0077361	78.481	395	49	4	1	389	20	384	0.0	618
Msa1117050	MtrunA17_Chr3g0077361	47.656	256	121	5	399	651	255	500	1.39e-56	203
Msa1117050	MtrunA17_Chr3g0077361	50.000	242	108	5	3	241	269	500	3.20e-56	202
Msa1117050	MtrunA17_Chr3g0077361	58.278	151	57	3	669	815	35	183	1.63e-45	171
Msa1117050	MtrunA17_Chr3g0077361	55.629	151	56	4	259	400	35	183	4.29e-41	158
Msa1117050	MtrunA17_Chr4g0009491	71.944	360	76	3	445	799	11	350	1.97e-179	524
Msa1117050	MtrunA17_Chr4g0009491	70.556	360	76	4	35	384	11	350	1.03e-173	509
Msa1117050	MtrunA17_Chr4g0009491	43.750	416	146	9	278	605	11	426	1.82e-94	303
Msa1117050	MtrunA17_Chr4g0009491	50.450	222	81	4	3	195	205	426	4.81e-56	199

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1117050	AT3G18990.1	45.455	99	54	0	674	772	6	104	5.95e-22	98.6
Msa1117050	AT3G18990.1	44.681	94	52	0	264	357	6	99	1.28e-20	94.7
Msa1117050	AT3G18990.1	38.384	99	57	1	21	115	6	104	7.01e-16	80.5
Msa1117050	AT3G18990.1	38.384	99	57	1	431	525	6	104	7.01e-16	80.5
Msa1117050	AT3G18990.2	45.455	99	54	0	674	772	6	104	9.12e-22	98.6
Msa1117050	AT3G18990.2	44.681	94	52	0	264	357	6	99	1.87e-20	94.7
Msa1117050	AT3G18990.2	38.384	99	57	1	21	115	6	104	8.69e-16	80.5
Msa1117050	AT3G18990.2	38.384	99	57	1	431	525	6	104	8.69e-16	80.5
Msa1117050	AT4G01580.1	37.879	132	79	1	643	774	2	130	6.36e-21	92.0
Msa1117050	AT4G01580.1	37.121	132	75	2	233	359	2	130	2.30e-18	84.3

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Find 186 sgRNAs with CRISPR-Local

Find 845 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGCGTCAGGTGCATTG+TGG	0.605314	7_3:-65170890	Msa1117050:CDS
AAAATGAGATTGAAGAAACG+CGG	0.691481	7_3:-65171214	Msa1117050:CDS
AAAATGAGATTGAAGAAACG+CGG	0.691481	7_3:-65183480	Msa1117050:CDS
AAAATTCTCAAGGAAAGCAG+TGG	0.599450	7_3:-65172233	Msa1117050:CDS
AAAATTCTCAAGGAAAGCAG+TGG	0.599450	7_3:-65184499	Msa1117050:CDS
AAATACTATTCGCTAGGATA+TGG	0.326079	7_3:-65171151	Msa1117050:CDS
AAATACTATTCGCTAGGATA+TGG	0.326079	7_3:-65183417	Msa1117050:CDS
AAATGTTTGGAGACTTTCTC+CGG	0.480142	7_3:+65173530	None:intergenic
AAATGTTTGGAGACTTTCTC+CGG	0.480142	7_3:+65185796	None:intergenic
AAATTCTCAAGGAAAGCAGT+GGG	0.637805	7_3:-65172232	Msa1117050:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTTTAACTCTTCAACTTTC+TGG	-	chr7_3:65171011-65171030	Msa1117050:CDS	30.0%
TAGGATCAAACTTCAAAAGA+GGG	+	chr7_3:65171510-65171529	None:intergenic	30.0%
ATAGGATCAAACTTCAAAAG+AGG	+	chr7_3:65171511-65171530	None:intergenic	30.0%
CTCATCAGTTATACTACTAT+AGG	+	chr7_3:65171529-65171548	None:intergenic	30.0%
AATCTTCTTATAAACTCGTC+TGG	+	chr7_3:65171573-65171592	None:intergenic	30.0%
GACGAGTTTATAAGAAGATT+TGG	-	chr7_3:65171573-65171592	Msa1117050:intron	30.0%
TAGTGGTGAGAAAAACATAA+AGG	-	chr7_3:65171836-65171855	Msa1117050:CDS	30.0%
AGTGGTGAGAAAAACATAAA+GGG	-	chr7_3:65171837-65171856	Msa1117050:CDS	30.0%
TTTCATTCAAAATACATGGG+CGG	-	chr7_3:65171924-65171943	Msa1117050:intron	30.0%
TATTGTGTATTGCATGCAAA+AGG	-	chr7_3:65172128-65172147	Msa1117050:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	65170763	65185857	65170763	ID=Msa1117050;Name=Msa1117050
chr7_3	mRNA	65170763	65185857	65170763	ID=Msa1117050-mRNA-1;Parent=Msa1117050;Name=Msa1117050-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|0.33\|0.15\|1\|0.83\|0.61\|13\|0\|873
chr7_3	exon	65185753	65185857	65185753	ID=Msa1117050-mRNA-1:exon:15471;Parent=Msa1117050-mRNA-1
chr7_3	exon	65184888	65185056	65184888	ID=Msa1117050-mRNA-1:exon:15470;Parent=Msa1117050-mRNA-1
chr7_3	exon	65184667	65184875	65184667	ID=Msa1117050-mRNA-1:exon:15469;Parent=Msa1117050-mRNA-1
chr7_3	exon	65184334	65184579	65184334	ID=Msa1117050-mRNA-1:exon:15468;Parent=Msa1117050-mRNA-1
chr7_3	exon	65184102	65184206	65184102	ID=Msa1117050-mRNA-1:exon:15467;Parent=Msa1117050-mRNA-1
chr7_3	exon	65183359	65183586	65183359	ID=Msa1117050-mRNA-1:exon:15466;Parent=Msa1117050-mRNA-1
chr7_3	exon	65183205	65183343	65183205	ID=Msa1117050-mRNA-1:exon:15465;Parent=Msa1117050-mRNA-1
chr7_3	exon	65173487	65173620	65173487	ID=Msa1117050-mRNA-1:exon:15464;Parent=Msa1117050-mRNA-1
chr7_3	exon	65172622	65172790	65172622	ID=Msa1117050-mRNA-1:exon:15463;Parent=Msa1117050-mRNA-1
chr7_3	exon	65172401	65172609	65172401	ID=Msa1117050-mRNA-1:exon:15462;Parent=Msa1117050-mRNA-1
chr7_3	exon	65172068	65172313	65172068	ID=Msa1117050-mRNA-1:exon:15461;Parent=Msa1117050-mRNA-1
chr7_3	exon	65171836	65171940	65171836	ID=Msa1117050-mRNA-1:exon:15460;Parent=Msa1117050-mRNA-1
chr7_3	exon	65170763	65171320	65170763	ID=Msa1117050-mRNA-1:exon:15459;Parent=Msa1117050-mRNA-1
chr7_3	CDS	65185753	65185857	65185753	ID=Msa1117050-mRNA-1:cds;Parent=Msa1117050-mRNA-1
chr7_3	CDS	65184888	65185056	65184888	ID=Msa1117050-mRNA-1:cds;Parent=Msa1117050-mRNA-1
chr7_3	CDS	65184667	65184875	65184667	ID=Msa1117050-mRNA-1:cds;Parent=Msa1117050-mRNA-1
chr7_3	CDS	65184334	65184579	65184334	ID=Msa1117050-mRNA-1:cds;Parent=Msa1117050-mRNA-1
chr7_3	CDS	65184102	65184206	65184102	ID=Msa1117050-mRNA-1:cds;Parent=Msa1117050-mRNA-1
chr7_3	CDS	65183359	65183586	65183359	ID=Msa1117050-mRNA-1:cds;Parent=Msa1117050-mRNA-1
chr7_3	CDS	65183205	65183343	65183205	ID=Msa1117050-mRNA-1:cds;Parent=Msa1117050-mRNA-1
chr7_3	CDS	65173487	65173620	65173487	ID=Msa1117050-mRNA-1:cds;Parent=Msa1117050-mRNA-1
chr7_3	CDS	65172622	65172790	65172622	ID=Msa1117050-mRNA-1:cds;Parent=Msa1117050-mRNA-1
chr7_3	CDS	65172401	65172609	65172401	ID=Msa1117050-mRNA-1:cds;Parent=Msa1117050-mRNA-1
chr7_3	CDS	65172068	65172313	65172068	ID=Msa1117050-mRNA-1:cds;Parent=Msa1117050-mRNA-1
chr7_3	CDS	65171836	65171940	65171836	ID=Msa1117050-mRNA-1:cds;Parent=Msa1117050-mRNA-1
chr7_3	CDS	65170763	65171320	65170763	ID=Msa1117050-mRNA-1:cds;Parent=Msa1117050-mRNA-1

Gene Sequence

>Msa1117050

ATGAAGCCTCGCCAGAGAAGATGTGTTCCGTCACCGGCGCCGGAGAAAGTCTCCAAACATTTTATGAAAGCCATACTTCCTCCTCCTGATCATATAAAAGAAATACGAATTCCAGACGAGTTTATAAGAAGATTTGGGAACGAACTCAAGAATGTTGCTATGATTACTGTGCCTGATGGCCGTGAATGGGAAATGGGACTGACGAAATGCGGCGAGCACATTTTCCTTAGCAACAATTGGCAACAGTTTGCAGAATACTATTCTATTCATTATGATTTCAATTATCAAGGGAATTCAAAGTTTAATGTTGTTATATATGATACAACTTCTGTTGAGATTTCTTATCCATTCCAAACTCAGAGTACTAGTGGTGAGAAAAACATAAAGGGTCCTAACTTTGCTTCCAGAAGAGCAAAATGTGCTGCCAGTGAATTCAACCCAAAAAACCCTTATTTTCATTCAAAATACATGGGCGGTTTTGCATATGTTCCTTCTAGTTTTGCTGAAAAGTATCTGACGGTAAAGGTTCCGTTCAAGCTCCAAAATTCTCAAGGAAAGCAGTGGGAAGTTTATTGTGTATTGCATGCAAAAGGAAAATCACAAATGCGAATAAGCTGTGGATTTTGTAAATTTGCAAGGGAAAACAATCTATTAAAGGGAGTTACATATGTGTTTGAGCTGACCAAGAGGAAGCCTGTTGTTGTTCTACAAGTCACAGCGCTTTGCACGCCTCCACAGGGAAGGTCTGCCCAGTCAATGACAGAAAAGGAAGTAAGGGAATCCAAACATTTTAAGAAGGCCATACTTCCCTCGCCCATTCATGACAAAGAGATAAGAATTCCGGATGAGTTTATAACAAGATTTGGGAACGAACTCAAAGATGTTGCTACAGTTACAGTTCCCGATGGTCGTGATTGGAAAATGAGATTGAAGAAACGCGGCAATGACATTTTCTTTTACAATGAATGGCAAGAGTTTGCAAAATACTATTCGCTAGGATATGGTTGCTACCTATCTTTCGAATATGAAGGGAATTCAAAGTTTAGTGTTATTCTGTTTGACATTTGCTATCCATTAAAAACTCCAAGTGTTAATGGAGAACCAAACACAAAGTGTGCTAGTCCTAGGAAAGGGTCCAAGGTTGAAACAAGTGAGTGTCATGGGAAAAATCCTGTCACTGCTAGGGCCAAGGGTCGTGGAGTTATCATTATTATTTGCAACAAACAAACAATGAAGCCTCGCCAGAGAAGATGTGTTCCGTCACCGGCGCCGGAGAAAGTCTCCAAACATTTTATGAAAGCCATACTTCCTCCTCCTGATCATATAAAAGAAATACGAATTCCAGACGAGTTTATAAGAAGATTTGGGAACGAACTCAAGAATGTTGCTATGATTACTGTGCCTGATGGCCGTGAATGGGAAATGGGACTGACGAAATGCGGCGAGCACATTTTCCTTAGCAACAATTGGCAACAGTTTGCAGAATACTATTCTATTCATTATGATTTCAATTATCAAGGGAATTCAAAGTTTAATGTTGTTATATATGATACAACTTCTGTTGAGATTTCTTATCCATTCCAAACTCAGAGTACTAGTGGTGAGAAAAACATAAAGGGTCCTAACTTTGCTTCCAGAAGAGCAAAATGTGCTGCCAGTGAATTCAACCCAAAAAACCCTTATTTTCATTCAAAATACATGGGCGGTTTTGCATATGTTCCTTCTAGTTTTGCTGAAAAGTATCTGACGGTAAAGGTTCCGTTCAAGCTCCAAAATTCTCAAGGAAAGCAGTGGGAAGTTTATTGTGTATTGCATGCAAAAGGAAAATCACAAATGCGAATAAGCTGTGGATTTTGTAAATTTGCAAGGGAAAACAATCTATTAAAGGGAGTTACATATGTGTTTGAGCTGACCAAGAGGAAGCCTGTTGTTGTTCTACAAGTCACAGCGCTTTGCACGCCTCCACAGGGAAGGTCTGCCCAGTCAATGACAGAAAAGGAAGTAAGGGAATCCAAACATTTTAAGAAGGCCATACTTCCCTCGCCCATTCATGACAAAGAGATAAGAATTCCGGATGAGTTTATAACAAGATTTGGGAACGAACTCAAAGATGTTGCTACAGTTACAGTTCCCGATGGTCGTGATTGGAAAATGAGATTGAAGAAACGCGGCAATGACATTTTCTTTTACAATGAATGGCAAGAGTTTGCAAAATACTATTCGCTAGGATATGGTTGCTACCTATCTTTCGAATATGAAGGGAATTCAAAGTTTAGTGTTATTCTGTTTGACGTAACTTCTGTTGAGATTTGCTATCCATTAAAAACTCCAAGTGTTAATGGAGAACCAAACACAAAGTGTGCTAGTCCTAGGAAAGGGTCCAAGGTTGAAACAAGTGAGTGTCATGGGAAAAATCCTGTCACTGCTAGGGCCAAGGGTCGTGGAGGTGCAGGAACCTCAAAACGCCAAAAAAAAAGGCGTCAGGTGCATTGTGGATCATGTGGGCTTGTGGGGCATAACAGACAGACTTGTACCGGTTTGACAAATGGCAAAGGACTGGATGATGAAAGTGAATTTACCAATGGGGACGAGGATTATGACGATGATGGTTACTCTGAATAA

Protein sequence

>Msa1117050

MKPRQRRCVPSPAPEKVSKHFMKAILPPPDHIKEIRIPDEFIRRFGNELKNVAMITVPDGREWEMGLTKCGEHIFLSNNWQQFAEYYSIHYDFNYQGNSKFNVVIYDTTSVEISYPFQTQSTSGEKNIKGPNFASRRAKCAASEFNPKNPYFHSKYMGGFAYVPSSFAEKYLTVKVPFKLQNSQGKQWEVYCVLHAKGKSQMRISCGFCKFARENNLLKGVTYVFELTKRKPVVVLQVTALCTPPQGRSAQSMTEKEVRESKHFKKAILPSPIHDKEIRIPDEFITRFGNELKDVATVTVPDGRDWKMRLKKRGNDIFFYNEWQEFAKYYSLGYGCYLSFEYEGNSKFSVILFDICYPLKTPSVNGEPNTKCASPRKGSKVETSECHGKNPVTARAKGRGVIIIICNKQTMKPRQRRCVPSPAPEKVSKHFMKAILPPPDHIKEIRIPDEFIRRFGNELKNVAMITVPDGREWEMGLTKCGEHIFLSNNWQQFAEYYSIHYDFNYQGNSKFNVVIYDTTSVEISYPFQTQSTSGEKNIKGPNFASRRAKCAASEFNPKNPYFHSKYMGGFAYVPSSFAEKYLTVKVPFKLQNSQGKQWEVYCVLHAKGKSQMRISCGFCKFARENNLLKGVTYVFELTKRKPVVVLQVTALCTPPQGRSAQSMTEKEVRESKHFKKAILPSPIHDKEIRIPDEFITRFGNELKDVATVTVPDGRDWKMRLKKRGNDIFFYNEWQEFAKYYSLGYGCYLSFEYEGNSKFSVILFDVTSVEICYPLKTPSVNGEPNTKCASPRKGSKVETSECHGKNPVTARAKGRGGAGTSKRQKKRRQVHCGSCGLVGHNRQTCTGLTNGKGLDDESEFTNGDEDYDDDGYSE*