Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1117490 | XP_039683935.1 | 87.607 | 234 | 25 | 2 | 1 | 230 | 1 | 234 | 1.78e-141 | 405 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1117490 | A0A396H4J8 | 87.607 | 234 | 25 | 2 | 1 | 230 | 1 | 234 | 8.52e-142 | 405 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1117490 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1117490 | MtrunA17_Chr7g0255511 | 87.607 | 234 | 25 | 2 | 1 | 230 | 1 | 234 | 1.64e-145 | 405 |
| Msa1117490 | MtrunA17_Chr7g0225091 | 77.778 | 234 | 34 | 3 | 1 | 230 | 1 | 220 | 2.18e-127 | 358 |
| Msa1117490 | MtrunA17_Chr7g0225121 | 75.641 | 234 | 31 | 3 | 1 | 230 | 1 | 212 | 5.65e-123 | 347 |
| Msa1117490 | MtrunA17_Chr7g0222361 | 76.733 | 202 | 45 | 2 | 31 | 230 | 1 | 202 | 4.89e-114 | 324 |
| Msa1117490 | MtrunA17_Chr7g0222371 | 74.380 | 121 | 26 | 3 | 19 | 134 | 1 | 121 | 1.51e-60 | 186 |
| Msa1117490 | MtrunA17_Chr1g0149811 | 32.558 | 172 | 97 | 6 | 76 | 230 | 93 | 262 | 3.56e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1117490 | AT1G66140.1 | 29.121 | 182 | 99 | 4 | 76 | 230 | 82 | 260 | 5.27e-11 | 61.2 |
Find 47 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAATTTCTTTGGAAGAAT+TGG | 0.283176 | 7_3:-65891504 | None:intergenic |
| AAGAAATGGAGAAATTTCTT+TGG | 0.320928 | 7_3:-65891513 | None:intergenic |
| CTTAAGGGTCAAATCAATCT+TGG | 0.345189 | 7_3:-65892011 | None:intergenic |
| TATATATTAATCAAAGCTTA+AGG | 0.360864 | 7_3:-65892027 | None:intergenic |
| TCCAAGCATTGTTAAAAGCT+TGG | 0.375606 | 7_3:-65891679 | None:intergenic |
| CACCATCTCTCCATTCCATA+TGG | 0.384400 | 7_3:+65891739 | Msa1117490:CDS |
| ATTGATATCCACACCCCATA+TGG | 0.402297 | 7_3:-65891754 | None:intergenic |
| AATTACTCAAATGGTGATCA+AGG | 0.411277 | 7_3:+65891704 | Msa1117490:CDS |
| ATGCTGAAACTCAGAATTCA+AGG | 0.417689 | 7_3:+65891450 | Msa1117490:CDS |
| CTCCTTCCCCTTGTTACACA+TGG | 0.424416 | 7_3:-65891345 | None:intergenic |
| AACAATATGATTCAGCATGC+AGG | 0.425567 | 7_3:+65891788 | Msa1117490:CDS |
| TGAAGTTGGTTCAATTTCAA+AGG | 0.450392 | 7_3:+65891928 | Msa1117490:CDS |
| GCTTGGATAAATTACTCAAA+TGG | 0.450589 | 7_3:+65891695 | Msa1117490:CDS |
| TGCTAGTGATGAAGTTGATC+AGG | 0.453101 | 7_3:+65891958 | Msa1117490:CDS |
| AATATGTCTGAGATTGAAGT+TGG | 0.464395 | 7_3:+65891914 | Msa1117490:CDS |
| ACCATCTCTCCATTCCATAT+GGG | 0.464694 | 7_3:+65891740 | Msa1117490:CDS |
| TTCACTAGTCCACAGGCATT+AGG | 0.474635 | 7_3:+65891608 | Msa1117490:CDS |
| TTGGATTCATTGTTGATCAC+AGG | 0.479894 | 7_3:+65891540 | Msa1117490:CDS |
| GGTAGCCAATTTGAAGATGA+TGG | 0.482136 | 7_3:+65891385 | Msa1117490:CDS |
| GAAGTTGGTTCAATTTCAAA+GGG | 0.492022 | 7_3:+65891929 | Msa1117490:CDS |
| CTCTGACGCCATGTGTAACA+AGG | 0.498945 | 7_3:+65891337 | None:intergenic |
| GGAGAAGGTGAATGAAGAAT+CGG | 0.503708 | 7_3:+65891364 | Msa1117490:CDS |
| CAAGAGAAAGGCAATCGTGC+TGG | 0.504921 | 7_3:+65891418 | Msa1117490:CDS |
| TCACAGGAAAGGAATTCAAA+AGG | 0.505285 | 7_3:+65891556 | Msa1117490:CDS |
| ATATATTAATCAAAGCTTAA+GGG | 0.526886 | 7_3:-65892026 | None:intergenic |
| TCTCCATTCCATATGGGGTG+TGG | 0.536840 | 7_3:+65891746 | Msa1117490:CDS |
| GGAAAATCAGAACAAGAGAA+AGG | 0.539450 | 7_3:+65891406 | Msa1117490:CDS |
| TTCATTGTTGATCACAGGAA+AGG | 0.555662 | 7_3:+65891545 | Msa1117490:CDS |
| TTGAATTCTGAGTTTCAGCA+TGG | 0.556814 | 7_3:-65891448 | None:intergenic |
| ACAATATGATTCAGCATGCA+GGG | 0.561522 | 7_3:+65891789 | Msa1117490:CDS |
| CCCCATATGGAATGGAGAGA+TGG | 0.561790 | 7_3:-65891741 | None:intergenic |
| GGGTGTGGATATCAATATTG+TGG | 0.571463 | 7_3:+65891761 | Msa1117490:CDS |
| CGCCATGTGTAACAAGGGGA+AGG | 0.573945 | 7_3:+65891343 | None:intergenic |
| CAGAAAGTTCACTAGTCCAC+AGG | 0.575345 | 7_3:+65891601 | Msa1117490:CDS |
| TGAATTCTGAGTTTCAGCAT+GGG | 0.580885 | 7_3:-65891447 | None:intergenic |
| ATTGCAACATATTTCAGAGA+TGG | 0.581211 | 7_3:+65891847 | Msa1117490:CDS |
| GTGTAACAAGGGGAAGGAGA+AGG | 0.590077 | 7_3:+65891349 | Msa1117490:CDS |
| GTTGGTTCAATTTCAAAGGG+AGG | 0.592252 | 7_3:+65891932 | Msa1117490:CDS |
| TATCCACACCCCATATGGAA+TGG | 0.594771 | 7_3:-65891749 | None:intergenic |
| CCATCTCTCCATTCCATATG+GGG | 0.598148 | 7_3:+65891741 | Msa1117490:CDS |
| ATTACTCAAATGGTGATCAA+GGG | 0.605537 | 7_3:+65891705 | Msa1117490:CDS |
| GGAAGACAAAGAGACTCAAG+AGG | 0.634668 | 7_3:+65891979 | Msa1117490:CDS |
| TCTGACGCCATGTGTAACAA+GGG | 0.635062 | 7_3:+65891338 | None:intergenic |
| TGATGACCACCTAATGCCTG+TGG | 0.637178 | 7_3:-65891617 | None:intergenic |
| ACTAGTCCACAGGCATTAGG+TGG | 0.661075 | 7_3:+65891611 | Msa1117490:CDS |
| AGACAAAGAGACTCAAGAGG+AGG | 0.673262 | 7_3:+65891982 | Msa1117490:CDS |
| CTGACGCCATGTGTAACAAG+GGG | 0.676472 | 7_3:+65891339 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTTCCATCATCTTCAAAT+TGG | - | chr7_3:65891393-65891412 | None:intergenic | 25.0% |
| !! | TTCTTCTTCAACATCTTTTT+GGG | - | chr7_3:65891480-65891499 | None:intergenic | 25.0% |
| !! | ATTCTTCTTCAACATCTTTT+TGG | - | chr7_3:65891481-65891500 | None:intergenic | 25.0% |
| !! | AGAAATTTCTTTGGAAGAAT+TGG | - | chr7_3:65891507-65891526 | None:intergenic | 25.0% |
| ! | AAGAAATGGAGAAATTTCTT+TGG | - | chr7_3:65891516-65891535 | None:intergenic | 25.0% |
| !! | ATTTCTCCATTTCTTCTTTT+TGG | + | chr7_3:65891521-65891540 | Msa1117490:CDS | 25.0% |
| ! | ATGAATCCAAAAAGAAGAAA+TGG | - | chr7_3:65891530-65891549 | None:intergenic | 25.0% |
| ! | TCTTCTTCAACATCTTTTTG+GGG | - | chr7_3:65891479-65891498 | None:intergenic | 30.0% |
| TAGAGAGAAGTCTCAAAAAA+AGG | + | chr7_3:65891654-65891673 | Msa1117490:CDS | 30.0% | |
| GCTTGGATAAATTACTCAAA+TGG | + | chr7_3:65891695-65891714 | Msa1117490:CDS | 30.0% | |
| AATTACTCAAATGGTGATCA+AGG | + | chr7_3:65891704-65891723 | Msa1117490:CDS | 30.0% | |
| ATTACTCAAATGGTGATCAA+GGG | + | chr7_3:65891705-65891724 | Msa1117490:CDS | 30.0% | |
| ! | TTTTATGCAGGGAATTTGTA+TGG | + | chr7_3:65891821-65891840 | Msa1117490:CDS | 30.0% |
| ATTGCAACATATTTCAGAGA+TGG | + | chr7_3:65891847-65891866 | Msa1117490:CDS | 30.0% | |
| ! | AATATGTCTGAGATTGAAGT+TGG | + | chr7_3:65891914-65891933 | Msa1117490:CDS | 30.0% |
| ! | TGAAGTTGGTTCAATTTCAA+AGG | + | chr7_3:65891928-65891947 | Msa1117490:CDS | 30.0% |
| ! | GAAGTTGGTTCAATTTCAAA+GGG | + | chr7_3:65891929-65891948 | Msa1117490:CDS | 30.0% |
| GGAAAATCAGAACAAGAGAA+AGG | + | chr7_3:65891406-65891425 | Msa1117490:CDS | 35.0% | |
| TGAATTCTGAGTTTCAGCAT+GGG | - | chr7_3:65891450-65891469 | None:intergenic | 35.0% | |
| TTGAATTCTGAGTTTCAGCA+TGG | - | chr7_3:65891451-65891470 | None:intergenic | 35.0% | |
| ATGCTGAAACTCAGAATTCA+AGG | + | chr7_3:65891450-65891469 | Msa1117490:CDS | 35.0% | |
| TTGGATTCATTGTTGATCAC+AGG | + | chr7_3:65891540-65891559 | Msa1117490:CDS | 35.0% | |
| TTCATTGTTGATCACAGGAA+AGG | + | chr7_3:65891545-65891564 | Msa1117490:CDS | 35.0% | |
| TCACAGGAAAGGAATTCAAA+AGG | + | chr7_3:65891556-65891575 | Msa1117490:CDS | 35.0% | |
| ! | TCCAAGCTTTTAACAATGCT+TGG | + | chr7_3:65891678-65891697 | Msa1117490:CDS | 35.0% |
| ! | TCCAAGCATTGTTAAAAGCT+TGG | - | chr7_3:65891682-65891701 | None:intergenic | 35.0% |
| AACAATATGATTCAGCATGC+AGG | + | chr7_3:65891788-65891807 | Msa1117490:CDS | 35.0% | |
| ACAATATGATTCAGCATGCA+GGG | + | chr7_3:65891789-65891808 | Msa1117490:CDS | 35.0% | |
| ! | GGTCATCAAACTTTTATGCA+GGG | + | chr7_3:65891810-65891829 | Msa1117490:CDS | 35.0% |
| CTTAAGGGTCAAATCAATCT+TGG | - | chr7_3:65892014-65892033 | None:intergenic | 35.0% | |
| GGAGAAGGTGAATGAAGAAT+CGG | + | chr7_3:65891364-65891383 | Msa1117490:CDS | 40.0% | |
| GGTAGCCAATTTGAAGATGA+TGG | + | chr7_3:65891385-65891404 | Msa1117490:CDS | 40.0% | |
| ACCATCTCTCCATTCCATAT+GGG | + | chr7_3:65891740-65891759 | Msa1117490:CDS | 40.0% | |
| ATTGATATCCACACCCCATA+TGG | - | chr7_3:65891757-65891776 | None:intergenic | 40.0% | |
| GGGTGTGGATATCAATATTG+TGG | + | chr7_3:65891761-65891780 | Msa1117490:CDS | 40.0% | |
| ! | GGGTCATCAAACTTTTATGC+AGG | + | chr7_3:65891809-65891828 | Msa1117490:CDS | 40.0% |
| ! | GTTGGTTCAATTTCAAAGGG+AGG | + | chr7_3:65891932-65891951 | Msa1117490:CDS | 40.0% |
| ! | TGCTAGTGATGAAGTTGATC+AGG | + | chr7_3:65891958-65891977 | Msa1117490:CDS | 40.0% |
| CAGAAAGTTCACTAGTCCAC+AGG | + | chr7_3:65891601-65891620 | Msa1117490:CDS | 45.0% | |
| TTCACTAGTCCACAGGCATT+AGG | + | chr7_3:65891608-65891627 | Msa1117490:CDS | 45.0% | |
| CACCATCTCTCCATTCCATA+TGG | + | chr7_3:65891739-65891758 | Msa1117490:CDS | 45.0% | |
| CCATCTCTCCATTCCATATG+GGG | + | chr7_3:65891741-65891760 | Msa1117490:CDS | 45.0% | |
| TATCCACACCCCATATGGAA+TGG | - | chr7_3:65891752-65891771 | None:intergenic | 45.0% | |
| GGAAGACAAAGAGACTCAAG+AGG | + | chr7_3:65891979-65891998 | Msa1117490:CDS | 45.0% | |
| AGACAAAGAGACTCAAGAGG+AGG | + | chr7_3:65891982-65892001 | Msa1117490:CDS | 45.0% | |
| GTGTAACAAGGGGAAGGAGA+AGG | + | chr7_3:65891349-65891368 | Msa1117490:CDS | 50.0% | |
| CAAGAGAAAGGCAATCGTGC+TGG | + | chr7_3:65891418-65891437 | Msa1117490:CDS | 50.0% | |
| ACTAGTCCACAGGCATTAGG+TGG | + | chr7_3:65891611-65891630 | Msa1117490:CDS | 50.0% | |
| TGATGACCACCTAATGCCTG+TGG | - | chr7_3:65891620-65891639 | None:intergenic | 50.0% | |
| CCCCATATGGAATGGAGAGA+TGG | - | chr7_3:65891744-65891763 | None:intergenic | 50.0% | |
| TCTCCATTCCATATGGGGTG+TGG | + | chr7_3:65891746-65891765 | Msa1117490:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 65891347 | 65892039 | 65891347 | ID=Msa1117490;Name=Msa1117490 |
| chr7_3 | mRNA | 65891347 | 65892039 | 65891347 | ID=Msa1117490-mRNA-1;Parent=Msa1117490;Name=Msa1117490-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|230 |
| chr7_3 | exon | 65891347 | 65892039 | 65891347 | ID=Msa1117490-mRNA-1:exon:15809;Parent=Msa1117490-mRNA-1 |
| chr7_3 | CDS | 65891347 | 65892039 | 65891347 | ID=Msa1117490-mRNA-1:cds;Parent=Msa1117490-mRNA-1 |
| Gene Sequence |
| Protein sequence |
