AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1120000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1120000 MtrunA17_Chr7g0251891 98.485 198 3 0 118 315 7 204 5.62e-147 411
Msa1120000 MtrunA17_Chr7g0251881 87.597 129 8 3 2 127 1 124 3.68e-68 209
Msa1120000 MtrunA17_Chr7g0237841 36.937 222 84 5 67 232 507 728 1.65e-33 130
Msa1120000 MtrunA17_Chr2g0281191 39.394 132 71 3 127 253 352 479 1.80e-20 92.0
Msa1120000 MtrunA17_Chr5g0432721 49.412 85 42 1 127 211 321 404 9.88e-20 89.7
Msa1120000 MtrunA17_Chr4g0012861 39.806 103 61 1 127 229 314 415 1.10e-19 89.7
Msa1120000 MtrunA17_Chr3g0107701 40.777 103 60 1 127 229 338 439 1.77e-19 89.4
Msa1120000 MtrunA17_Chr4g0047031 34.104 173 93 6 67 231 120 279 2.67e-19 87.0
Msa1120000 MtrunA17_Chr7g0244871 43.182 88 49 1 127 214 12 98 1.11e-18 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1120000 AT3G26790.1 46.479 355 108 14 1 315 1 313 1.18e-86 263
Msa1120000 AT3G24650.1 38.318 214 80 4 67 228 459 672 1.05e-35 137
Msa1120000 AT1G28300.1 49.606 127 64 0 119 245 162 288 2.52e-34 129
Msa1120000 AT2G30470.1 39.806 103 61 1 127 229 294 395 8.09e-21 93.6
Msa1120000 AT4G32010.2 34.104 173 98 5 127 296 281 440 1.65e-20 92.4
Msa1120000 AT4G32010.1 34.104 173 98 5 127 296 285 444 1.68e-20 92.4
Msa1120000 AT4G21550.2 38.835 103 60 2 127 229 327 426 7.70e-17 81.3
Msa1120000 AT4G21550.1 38.835 103 60 2 127 229 327 426 8.30e-17 81.6
Msa1120000 AT4G21550.3 38.835 103 60 2 127 229 327 426 8.74e-17 81.3
Msa1120000 AT1G13260.1 38.000 100 55 4 122 217 182 278 7.59e-11 62.8

Find 58 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTAGTTGCTGCTGCATT+TGG 0.326686 7_3:-69169208 Msa1120000:CDS
ATTGGATCAGTTGAAACATT+TGG 0.328924 7_3:-69166037 Msa1120000:CDS
TGCATTGCTACAGGTAATTC+AGG 0.347992 7_3:-69166272 Msa1120000:intron
ATGACAAACTACTCAAGAAT+TGG 0.349320 7_3:-69166055 Msa1120000:CDS
ACGCTTTGGAGACTCCATTA+TGG 0.373590 7_3:-69166421 Msa1120000:CDS
CTGCATTTGGAACTGTTCAT+AGG 0.379357 7_3:-69169195 Msa1120000:CDS
AACCGAGGCGTGTGGTTTCA+TGG 0.390478 7_3:-69169326 Msa1120000:CDS
ATTATCTTGGTAAACCATAA+TGG 0.396701 7_3:+69166407 None:intergenic
TACATCCGACTGTTATTGTT+AGG 0.408158 7_3:+69166576 None:intergenic
TATCTTTGTTGATGATTATG+AGG 0.427462 7_3:-69166191 Msa1120000:CDS
AACAACAACAACAAGATTAA+CGG 0.429296 7_3:-69169259 Msa1120000:CDS
AGCACGCATGGTCTACGCTT+TGG 0.431980 7_3:-69166435 Msa1120000:CDS
CCTTCCTGTCCTTGAGTCAA+AGG 0.435965 7_3:-69167006 Msa1120000:CDS
TTGTGATCAAGATGAGTATA+TGG 0.437591 7_3:-69166239 Msa1120000:CDS
AATTGTGATTGTGATTATCT+TGG 0.442600 7_3:+69166394 None:intergenic
AGAGGTGGAATTGGAGAGAT+TGG 0.443660 7_3:+69169070 None:intergenic
GAACTGTTCATAGGAAGAAA+AGG 0.451838 7_3:-69169186 Msa1120000:CDS
GATAGCAATGATGATGGATG+AGG 0.453480 7_3:-69169380 None:intergenic
AAAGTAGATAGCAATGATGA+TGG 0.461976 7_3:-69169386 None:intergenic
ATATAGATGGTCTTCATGTA+TGG 0.471743 7_3:-69166959 Msa1120000:CDS
ACAACAACAACAAGATTAAC+GGG 0.472192 7_3:-69169258 Msa1120000:CDS
TAGGAGAATGGTGTTGCCTA+AGG 0.474134 7_3:-69167125 Msa1120000:intron
CCTTCCTTTGACTCAAGGAC+AGG 0.475486 7_3:+69167002 None:intergenic
ATGTATGGAGTTTCAAGTAC+AGG 0.489515 7_3:-69166944 Msa1120000:intron
GAGGCGTGTGGTTTCATGGC+AGG 0.489847 7_3:-69169322 Msa1120000:CDS
GAATTCCTTCCTTTGACTCA+AGG 0.492444 7_3:+69166997 None:intergenic
CTCAGCATGAATGATATAGA+TGG 0.492894 7_3:-69166972 Msa1120000:CDS
TCACTTGATGACTTCTATTG+AGG 0.496600 7_3:-69165998 None:intergenic
ATGCTTGTATCATTCATTGC+AGG 0.499638 7_3:+69166139 None:intergenic
TAGGCAACACCATTCTCCTA+AGG 0.515359 7_3:+69167128 None:intergenic
AGATTAACGGGTCGGGTCAA+CGG 0.518369 7_3:-69169246 Msa1120000:CDS
TGCCATGAAACCACACGCCT+CGG 0.521021 7_3:+69169324 None:intergenic
GGAGACTTTGTTAGCACGCA+TGG 0.528164 7_3:-69166447 Msa1120000:CDS
TGTTAGCTCCCTTAGGAGAA+TGG 0.536299 7_3:-69167137 Msa1120000:CDS
CAACGGATCCATCATGATCA+TGG 0.538714 7_3:-69169229 Msa1120000:CDS
CTAGGGTTTGTCAACGTGAA+AGG 0.540011 7_3:-69169286 Msa1120000:CDS
GCAGGTGTTGAAGATGAACT+AGG 0.544873 7_3:-69169304 Msa1120000:CDS
CAACTAAACCATGATCATGA+TGG 0.545398 7_3:+69169221 None:intergenic
AACAACAAGATTAACGGGTC+GGG 0.549708 7_3:-69169253 Msa1120000:CDS
ATAGGAAGAAAAGGATGGCT+AGG 0.560627 7_3:-69169177 Msa1120000:CDS
CAACAACAAGATTAACGGGT+CGG 0.560654 7_3:-69169254 Msa1120000:CDS
ATAGCAATGATGATGGATGA+GGG 0.562602 7_3:-69169379 None:intergenic
ACAGTGATGTTAGCTCCCTT+AGG 0.565931 7_3:-69167144 Msa1120000:CDS
TTTAGGAAATCGATCATAGG+AGG 0.568666 7_3:-69167198 Msa1120000:intron
CAGGTGTTGAAGATGAACTA+GGG 0.583873 7_3:-69169303 Msa1120000:CDS
CCTGTCCTTGAGTCAAAGGA+AGG 0.585425 7_3:-69167002 Msa1120000:CDS
CCTTTGACTCAAGGACAGGA+AGG 0.601949 7_3:+69167006 None:intergenic
AGAAGGTAAGAAGAAAGTTG+TGG 0.604435 7_3:-69169356 Msa1120000:CDS
AGGCAACACCATTCTCCTAA+GGG 0.607377 7_3:+69167129 None:intergenic
ATGATGATGGATGAGGGAGA+AGG 0.610410 7_3:-69169373 Msa1120000:CDS
TGGAGAGATTGGCACGTGCG+AGG 0.625104 7_3:+69169081 None:intergenic
GTTCAGAAAACCGAGGCGTG+TGG 0.632033 7_3:-69169334 Msa1120000:CDS
TGTTCATAGGAAGAAAAGGA+TGG 0.632115 7_3:-69169182 Msa1120000:CDS
AAGTGGTGAATCATTTGAGA+TGG 0.633283 7_3:+69166096 None:intergenic
TAACTTGCAGAAAGCAGCAG+AGG 0.635910 7_3:-69167033 Msa1120000:intron
ATGTATGTACTTGAAAACAC+TGG 0.640244 7_3:-69166558 Msa1120000:intron
TTTGGCCTAACAATAACAGT+CGG 0.655066 7_3:-69166581 Msa1120000:CDS
AGTTGTGGTTCAGAAAACCG+AGG 0.753509 7_3:-69169341 Msa1120000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATACAAATAATTACATAAA+AGG - chr7_3:69166835-69166854 Msa1120000:intron 10.0%
!! ATACAAATAATTACATAAAA+GGG - chr7_3:69166836-69166855 Msa1120000:intron 10.0%
!! AGTAATAATATGTATAAAAA+TGG - chr7_3:69166989-69167008 Msa1120000:CDS 10.0%
!!! TGAATGAATAAAAATTATTT+TGG + chr7_3:69167102-69167121 None:intergenic 10.0%
!! AAAATCTATACATTTAACAT+CGG + chr7_3:69166510-69166529 None:intergenic 15.0%
!! TTATACATATTATTACTACT+TGG + chr7_3:69166986-69167005 None:intergenic 15.0%
!! ATATGTATAAAAATGGAAAA+AGG - chr7_3:69166996-69167015 Msa1120000:CDS 15.0%
!! TGTTGGATATAAATATAATA+TGG - chr7_3:69167981-69168000 Msa1120000:intron 15.0%
!! AAATTAGTTAATACCATAAA+TGG - chr7_3:69168130-69168149 Msa1120000:intron 15.0%
!! AAATTTAATGCAATTACATT+TGG - chr7_3:69168510-69168529 Msa1120000:intron 15.0%
!!! TTAGTTTGTTAAATATTTTC+AGG - chr7_3:69168905-69168924 Msa1120000:intron 15.0%
!!! AAGTAATGCTTATAGATTTT+TGG - chr7_3:69167205-69167224 Msa1120000:intron 20.0%
!! GTTTAACTATATCAATGTAT+AGG - chr7_3:69167244-69167263 Msa1120000:intron 20.0%
!!! ATTTTGGAAGTTGATTTTAT+GGG - chr7_3:69167469-69167488 Msa1120000:intron 20.0%
!!! AATTACGAAAGTTGATTTTA+CGG - chr7_3:69167635-69167654 Msa1120000:intron 20.0%
!! AATTTGTGTATTTCAAGATA+AGG - chr7_3:69167897-69167916 Msa1120000:intron 20.0%
!!! ACAATTTTCATTCTGTTTTT+AGG - chr7_3:69168158-69168177 Msa1120000:intron 20.0%
!! TATGACAGGAAAAAAAATAT+TGG + chr7_3:69169081-69169100 None:intergenic 20.0%
! AACAACAACAACAAGATTAA+CGG - chr7_3:69166114-69166133 Msa1120000:CDS 25.0%
!!! ATTTTTGAGATGAATAGTGA+TGG + chr7_3:69166244-69166263 None:intergenic 25.0%
! TACTAGTAGTTTGATCTTAA+TGG - chr7_3:69166568-69166587 Msa1120000:CDS 25.0%
! TTGTATGCAAATAGATCAAT+AGG - chr7_3:69166598-69166617 Msa1120000:intron 25.0%
! AAAGACTAATCTCTTGAATT+CGG - chr7_3:69166682-69166701 Msa1120000:intron 25.0%
!! TTATCTTCATTTTCTTGTGT+GGG + chr7_3:69166789-69166808 None:intergenic 25.0%
!! TTTATCTTCATTTTCTTGTG+TGG + chr7_3:69166790-69166809 None:intergenic 25.0%
! ATAATTACATAAAAGGGTAG+CGG - chr7_3:69166842-69166861 Msa1120000:intron 25.0%
! ATAAAAATGGAAAAAGGAAG+AGG - chr7_3:69167002-69167021 Msa1120000:CDS 25.0%
! ATAGTTAAACCAAAAAGACA+AGG + chr7_3:69167234-69167253 None:intergenic 25.0%
! AATTCAGAACTCTATTCAAT+GGG - chr7_3:69167273-69167292 Msa1120000:intron 25.0%
!! AATTCATAAGTCGATTTCAT+AGG - chr7_3:69167420-69167439 Msa1120000:intron 25.0%
!!! CATTTTGGAAGTTGATTTTA+TGG - chr7_3:69167468-69167487 Msa1120000:intron 25.0%
!!! ATTTCGAAAGTCGATTTTAT+TGG - chr7_3:69167567-69167586 Msa1120000:intron 25.0%
!!! ATTACGAAAGTTGATTTTAC+GGG - chr7_3:69167636-69167655 Msa1120000:intron 25.0%
! AAACAGAATGAAAATTGTCA+AGG + chr7_3:69168156-69168175 None:intergenic 25.0%
!!! ATCGTACTTTAAAGTTGATT+TGG - chr7_3:69168612-69168631 Msa1120000:intron 25.0%
!!! TCGTACTTTAAAGTTGATTT+GGG - chr7_3:69168613-69168632 Msa1120000:intron 25.0%
! ATAAGGAGAAAAATTTCTCA+AGG + chr7_3:69168686-69168705 None:intergenic 25.0%
!! TGAGAAATTTTTCTCCTTAT+TGG - chr7_3:69168686-69168705 Msa1120000:intron 25.0%
! TAAAGGAATGTAAACCAATA+AGG + chr7_3:69168703-69168722 None:intergenic 25.0%
!! TATTTCTATTTCAATACTGC+AGG - chr7_3:69168768-69168787 Msa1120000:intron 25.0%
! ATTATCTTGGTAAACCATAA+TGG + chr7_3:69168969-69168988 None:intergenic 25.0%
! AATTGTGATTGTGATTATCT+TGG + chr7_3:69168982-69169001 None:intergenic 25.0%
!!! TATCTTTGTTGATGATTATG+AGG - chr7_3:69169182-69169201 Msa1120000:CDS 25.0%
ACAACAACAACAAGATTAAC+GGG - chr7_3:69166115-69166134 Msa1120000:CDS 30.0%
! TTTTTATTATCTGTCCTCGT+TGG - chr7_3:69166406-69166425 Msa1120000:CDS 30.0%
!! TTTTAACTCATTGCTAGATG+AGG - chr7_3:69167026-69167045 Msa1120000:CDS 30.0%
!!! AAATTATTTTGGTCATTGCC+CGG + chr7_3:69167091-69167110 None:intergenic 30.0%
CAATTCAGAACTCTATTCAA+TGG - chr7_3:69167272-69167291 Msa1120000:intron 30.0%
ATTTCAGAAGTCCATTTCAT+GGG - chr7_3:69167322-69167341 Msa1120000:intron 30.0%
!! ATTTCGGAAGTCGATTTTAT+AGG - chr7_3:69167371-69167390 Msa1120000:intron 30.0%
!! ATTTCGGAAGTCGATTTTAT+GGG - chr7_3:69167371-69167390 Msa1120000:intron 30.0%
!! ATAAGAGCATCAAAGAGTTA+CGG + chr7_3:69167962-69167981 None:intergenic 30.0%
AGTAAATGCTGCAAAAAGTT+GGG + chr7_3:69168077-69168096 None:intergenic 30.0%
TAGTAAATGCTGCAAAAAGT+TGG + chr7_3:69168078-69168097 None:intergenic 30.0%
AAAATTGTCAAGGCCATTTA+TGG + chr7_3:69168146-69168165 None:intergenic 30.0%
!! GTTTTTAGGAAATCGATCAT+AGG - chr7_3:69168172-69168191 Msa1120000:intron 30.0%
!! TTTTCAACTCCTTTTGGAAA+AGG + chr7_3:69168209-69168228 None:intergenic 30.0%
GAAATGAAGTCTATAAGCAA+AGG + chr7_3:69168279-69168298 None:intergenic 30.0%
ATATAGATGGTCTTCATGTA+TGG - chr7_3:69168414-69168433 Msa1120000:intron 30.0%
GTCAATCATTCTTTATCTGA+CGG + chr7_3:69168581-69168600 None:intergenic 30.0%
! TGGTTTACATTCCTTTAAGA+TGG - chr7_3:69168706-69168725 Msa1120000:intron 30.0%
!! GGTTTACATTCCTTTAAGAT+GGG - chr7_3:69168707-69168726 Msa1120000:intron 30.0%
! TATTTCAATACTGCAGGTTT+TGG - chr7_3:69168774-69168793 Msa1120000:intron 30.0%
ATGTATGTACTTGAAAACAC+TGG - chr7_3:69168815-69168834 Msa1120000:intron 30.0%
AGCAAAGTTTAACTCTCAAA+TGG + chr7_3:69168874-69168893 None:intergenic 30.0%
TGAGCTTAACAAGAAAACTA+AGG - chr7_3:69169007-69169026 Msa1120000:intron 30.0%
TTGTGATCAAGATGAGTATA+TGG - chr7_3:69169134-69169153 Msa1120000:CDS 30.0%
!!! TGATTCACCACTTGATTTTT+TGG - chr7_3:69169287-69169306 Msa1120000:CDS 30.0%
!!! GATTCACCACTTGATTTTTT+GGG - chr7_3:69169288-69169307 Msa1120000:CDS 30.0%
ATGACAAACTACTCAAGAAT+TGG - chr7_3:69169318-69169337 Msa1120000:CDS 30.0%
ATTGGATCAGTTGAAACATT+TGG - chr7_3:69169336-69169355 Msa1120000:CDS 30.0%
AGAAGGTAAGAAGAAAGTTG+TGG - chr7_3:69166017-69166036 Msa1120000:CDS 35.0%
CAACTAAACCATGATCATGA+TGG + chr7_3:69166155-69166174 None:intergenic 35.0%
GAACTGTTCATAGGAAGAAA+AGG - chr7_3:69166187-69166206 Msa1120000:CDS 35.0%
TGTTCATAGGAAGAAAAGGA+TGG - chr7_3:69166191-69166210 Msa1120000:CDS 35.0%
GTAGTAGTAGTAGTAGAAGA+AGG + chr7_3:69166270-69166289 None:intergenic 35.0%
GAATGAAAAAGAGGTGGAAT+TGG + chr7_3:69166315-69166334 None:intergenic 35.0%
AGAAAAAAAACTCACACGTG+CGG + chr7_3:69166346-69166365 None:intergenic 35.0%
! AAGTTTTTGCAAATCCAACG+AGG + chr7_3:69166423-69166442 None:intergenic 35.0%
GGGTGTGTATAAATTAGTGT+AGG + chr7_3:69166769-69166788 None:intergenic 35.0%
!! TTTTGGTGACCTTGTCTTTT+TGG - chr7_3:69167222-69167241 Msa1120000:intron 35.0%
AACTCTATTCAATGGGTCAA+TGG - chr7_3:69167280-69167299 Msa1120000:intron 35.0%
CATTTCAGAAGTCCATTTCA+TGG - chr7_3:69167321-69167340 Msa1120000:intron 35.0%
CATGTATACGCTACTCATTT+CGG - chr7_3:69167306-69167325 Msa1120000:intron 35.0%
! CATGTATACGACACTCATTT+TGG - chr7_3:69167453-69167472 Msa1120000:intron 35.0%
CATGTATACACCACTCATTT+CGG - chr7_3:69167502-69167521 Msa1120000:intron 35.0%
!! CATTTCGGAAGTCGATTTTA+TGG - chr7_3:69167370-69167389 Msa1120000:intron 35.0%
!! AAGTTGATTTTACGGGTCAA+CGG - chr7_3:69167643-69167662 Msa1120000:intron 35.0%
! AAGCTGAAAACTCCTACTTT+TGG - chr7_3:69167849-69167868 Msa1120000:intron 35.0%
AGATTTAGGGAACCAAAAGT+AGG + chr7_3:69167864-69167883 None:intergenic 35.0%
!!! CTTTTGGTTCCCTAAATCTT+TGG - chr7_3:69167865-69167884 Msa1120000:intron 35.0%
ACTCTTTGATGCTCTTATGT+TGG - chr7_3:69167964-69167983 Msa1120000:intron 35.0%
TTTAGGAAATCGATCATAGG+AGG - chr7_3:69168175-69168194 Msa1120000:intron 35.0%
! GTTGAGATTCCTTTTCCAAA+AGG - chr7_3:69168197-69168216 Msa1120000:intron 35.0%
!! CACTGTTTTTCAACTCCTTT+TGG + chr7_3:69168215-69168234 None:intergenic 35.0%
ATAAGCAAAGGTCAAACCTT+AGG + chr7_3:69168267-69168286 None:intergenic 35.0%
CTCAGCATGAATGATATAGA+TGG - chr7_3:69168401-69168420 Msa1120000:intron 35.0%
! ATGTATGGAGTTTCAAGTAC+AGG - chr7_3:69168429-69168448 Msa1120000:intron 35.0%
CTATGTAAGTGTGTGACTAA+TGG + chr7_3:69168545-69168564 None:intergenic 35.0%
GTCAATCTTTCCCATCTTAA+AGG + chr7_3:69168720-69168739 None:intergenic 35.0%
AAGATGGGAAAGATTGACAA+TGG - chr7_3:69168722-69168741 Msa1120000:intron 35.0%
TTTGGCCTAACAATAACAGT+CGG - chr7_3:69168792-69168811 Msa1120000:intron 35.0%
TACATCCGACTGTTATTGTT+AGG + chr7_3:69168800-69168819 None:intergenic 35.0%
ATGCTTGTATCATTCATTGC+AGG + chr7_3:69169237-69169256 None:intergenic 35.0%
AAGTGGTGAATCATTTGAGA+TGG + chr7_3:69169280-69169299 None:intergenic 35.0%
!! CAGGTGTTGAAGATGAACTA+GGG - chr7_3:69166070-69166089 Msa1120000:CDS 40.0%
! CAACAACAAGATTAACGGGT+CGG - chr7_3:69166119-69166138 Msa1120000:CDS 40.0%
! AACAACAAGATTAACGGGTC+GGG - chr7_3:69166120-69166139 Msa1120000:CDS 40.0%
! CTGCATTTGGAACTGTTCAT+AGG - chr7_3:69166178-69166197 Msa1120000:CDS 40.0%
ATAGGAAGAAAAGGATGGCT+AGG - chr7_3:69166196-69166215 Msa1120000:CDS 40.0%
GAAAAAAAACTCACACGTGC+GGG + chr7_3:69166345-69166364 None:intergenic 40.0%
TGAATTCGGTTAGACCACAA+TGG - chr7_3:69166696-69166715 Msa1120000:intron 40.0%
AAGAACTTCGTCATCCATTG+TGG + chr7_3:69166713-69166732 None:intergenic 40.0%
GTGTAGGTCTGAGAATACAA+CGG + chr7_3:69166753-69166772 None:intergenic 40.0%
!! GTAGCGGATGTATCATGTAT+GGG - chr7_3:69166858-69166877 Msa1120000:intron 40.0%
AGGCAGAATCTATGTCTGTA+CGG - chr7_3:69167046-69167065 Msa1120000:intron 40.0%
GATATCGTTGACCCATGAAA+TGG + chr7_3:69167336-69167355 None:intergenic 40.0%
! AAATCGACTTCCGAAATGAG+TGG + chr7_3:69167368-69167387 None:intergenic 40.0%
TCTCGATCGTTTGTAGAGAT+TGG - chr7_3:69167682-69167701 Msa1120000:intron 40.0%
CTGATCTCTGTCCAATAACT+TGG - chr7_3:69167752-69167771 Msa1120000:intron 40.0%
ATCGAGTCGATCCAAGTTAT+TGG + chr7_3:69167766-69167785 None:intergenic 40.0%
CTTAGTCAATGTCTAGTCCT+TGG - chr7_3:69167826-69167845 Msa1120000:intron 40.0%
TTGGTTCCCTAAATCTTTGG+TGG - chr7_3:69167868-69167887 Msa1120000:intron 40.0%
AAAGAGTTACGGTGGTAAAG+AGG + chr7_3:69167951-69167970 None:intergenic 40.0%
GAATTCCTTCCTTTGACTCA+AGG + chr7_3:69168379-69168398 None:intergenic 40.0%
CTGCTATGTGCATACATTGT+TGG - chr7_3:69168453-69168472 Msa1120000:intron 40.0%
TGCATTGCTACAGGTAATTC+AGG - chr7_3:69169101-69169120 Msa1120000:CDS 40.0%
!!! TCACCACTTGATTTTTTGGG+TGG - chr7_3:69169291-69169310 Msa1120000:CDS 40.0%
GATCCACCCAAAAAATCAAG+TGG + chr7_3:69169297-69169316 None:intergenic 40.0%
!! ATGATGATGGATGAGGGAGA+AGG - chr7_3:69166000-69166019 Msa1120000:CDS 45.0%
AGTTGTGGTTCAGAAAACCG+AGG - chr7_3:69166032-69166051 Msa1120000:CDS 45.0%
!! GCAGGTGTTGAAGATGAACT+AGG - chr7_3:69166069-69166088 Msa1120000:CDS 45.0%
CTAGGGTTTGTCAACGTGAA+AGG - chr7_3:69166087-69166106 Msa1120000:CDS 45.0%
CAACGGATCCATCATGATCA+TGG - chr7_3:69166144-69166163 Msa1120000:CDS 45.0%
GGTTTAGTTGCTGCTGCATT+TGG - chr7_3:69166165-69166184 Msa1120000:CDS 45.0%
AGAGGTGGAATTGGAGAGAT+TGG + chr7_3:69166306-69166325 None:intergenic 45.0%
GGGAGAGAATGAAAAAGAGG+TGG + chr7_3:69166321-69166340 None:intergenic 45.0%
GGAGGGAGAGAATGAAAAAG+AGG + chr7_3:69166324-69166343 None:intergenic 45.0%
!! GGTAGCGGATGTATCATGTA+TGG - chr7_3:69166857-69166876 Msa1120000:intron 45.0%
ATGCGCAGTATTCTGACACA+AGG + chr7_3:69166963-69166982 None:intergenic 45.0%
!!! TTATTTTGGTCATTGCCCGG+TGG + chr7_3:69167088-69167107 None:intergenic 45.0%
CTCACGTGACTTTCGCAAAT+TGG + chr7_3:69167728-69167747 None:intergenic 45.0%
! GTAGGAGTTTTCAGCTTCCA+AGG + chr7_3:69167846-69167865 None:intergenic 45.0%
!! TCCCTAAATCTTTGGTGGTG+TGG - chr7_3:69167873-69167892 Msa1120000:intron 45.0%
CCCACACCACCAAAGATTTA+GGG + chr7_3:69167877-69167896 None:intergenic 45.0%
!! CCCTAAATCTTTGGTGGTGT+GGG - chr7_3:69167874-69167893 Msa1120000:intron 45.0%
TCCCACACCACCAAAGATTT+AGG + chr7_3:69167878-69167897 None:intergenic 45.0%
AGGCATGCTAGACAATGCTT+TGG - chr7_3:69167917-69167936 Msa1120000:intron 45.0%
GGCATGCTAGACAATGCTTT+GGG - chr7_3:69167918-69167937 Msa1120000:intron 45.0%
! AGAGCATCAAAGAGTTACGG+TGG + chr7_3:69167959-69167978 None:intergenic 45.0%
ACAGTGATGTTAGCTCCCTT+AGG - chr7_3:69168229-69168248 Msa1120000:intron 45.0%
TGTTAGCTCCCTTAGGAGAA+TGG - chr7_3:69168236-69168255 Msa1120000:intron 45.0%
AGGCAACACCATTCTCCTAA+GGG + chr7_3:69168247-69168266 None:intergenic 45.0%
TAGGCAACACCATTCTCCTA+AGG + chr7_3:69168248-69168267 None:intergenic 45.0%
!! TAGGAGAATGGTGTTGCCTA+AGG - chr7_3:69168248-69168267 Msa1120000:intron 45.0%
! TAACTTGCAGAAAGCAGCAG+AGG - chr7_3:69168340-69168359 Msa1120000:intron 45.0%
!! ACGCTTTGGAGACTCCATTA+TGG - chr7_3:69168952-69168971 Msa1120000:intron 45.0%
CCTGTAGCAATGCATATGAC+AGG + chr7_3:69169095-69169114 None:intergenic 45.0%
CCTGTCATATGCATTGCTAC+AGG - chr7_3:69169092-69169111 Msa1120000:CDS 45.0%
!! ATTCAAAAAAAAAAAAAAAT+TGG + chr7_3:69166631-69166650 None:intergenic 5.0%
! AGATTAACGGGTCGGGTCAA+CGG - chr7_3:69166127-69166146 Msa1120000:CDS 50.0%
AAAAACTCACACGTGCGGGA+GGG + chr7_3:69166341-69166360 None:intergenic 50.0%
AAAAAACTCACACGTGCGGG+AGG + chr7_3:69166342-69166361 None:intergenic 50.0%
TCTGTACGGAATTTGCCTCG+TGG - chr7_3:69167060-69167079 Msa1120000:intron 50.0%
AATTTGCCTCGTGGATCCAC+CGG - chr7_3:69167069-69167088 Msa1120000:intron 50.0%
CCTTTGACTCAAGGACAGGA+AGG + chr7_3:69168370-69168389 None:intergenic 50.0%
CCTTCCTGTCCTTGAGTCAA+AGG - chr7_3:69168367-69168386 Msa1120000:intron 50.0%
CCTTCCTTTGACTCAAGGAC+AGG + chr7_3:69168374-69168393 None:intergenic 50.0%
CCTGTCCTTGAGTCAAAGGA+AGG - chr7_3:69168371-69168390 Msa1120000:intron 50.0%
GGAGACTTTGTTAGCACGCA+TGG - chr7_3:69168926-69168945 Msa1120000:intron 50.0%
GTTCAGAAAACCGAGGCGTG+TGG - chr7_3:69166039-69166058 Msa1120000:CDS 55.0%
AACCGAGGCGTGTGGTTTCA+TGG - chr7_3:69166047-69166066 Msa1120000:CDS 55.0%
TGCCATGAAACCACACGCCT+CGG + chr7_3:69166052-69166071 None:intergenic 55.0%
ATTTGCCTCGTGGATCCACC+GGG - chr7_3:69167070-69167089 Msa1120000:intron 55.0%
AGCACGCATGGTCTACGCTT+TGG - chr7_3:69168938-69168957 Msa1120000:intron 55.0%
GAGGCGTGTGGTTTCATGGC+AGG - chr7_3:69166051-69166070 Msa1120000:CDS 60.0%
TGGAGAGATTGGCACGTGCG+AGG + chr7_3:69166295-69166314 None:intergenic 60.0%
CATTGCCCGGTGGATCCACG+AGG + chr7_3:69167078-69167097 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 69166000 69169395 69166000 ID=Msa1120000;Name=Msa1120000
chr7_3 mRNA 69166000 69169395 69166000 ID=Msa1120000-mRNA-1;Parent=Msa1120000;Name=Msa1120000-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0|1|1|1|6|0|315
chr7_3 exon 69169045 69169395 69169045 ID=Msa1120000-mRNA-1:exon:17492;Parent=Msa1120000-mRNA-1
chr7_3 exon 69167126 69167215 69167126 ID=Msa1120000-mRNA-1:exon:17491;Parent=Msa1120000-mRNA-1
chr7_3 exon 69166945 69167045 69166945 ID=Msa1120000-mRNA-1:exon:17490;Parent=Msa1120000-mRNA-1
chr7_3 exon 69166559 69166605 69166559 ID=Msa1120000-mRNA-1:exon:17489;Parent=Msa1120000-mRNA-1
chr7_3 exon 69166392 69166468 69166392 ID=Msa1120000-mRNA-1:exon:17488;Parent=Msa1120000-mRNA-1
chr7_3 exon 69166000 69166281 69166000 ID=Msa1120000-mRNA-1:exon:17487;Parent=Msa1120000-mRNA-1
chr7_3 CDS 69169045 69169395 69169045 ID=Msa1120000-mRNA-1:cds;Parent=Msa1120000-mRNA-1
chr7_3 CDS 69167126 69167215 69167126 ID=Msa1120000-mRNA-1:cds;Parent=Msa1120000-mRNA-1
chr7_3 CDS 69166945 69167045 69166945 ID=Msa1120000-mRNA-1:cds;Parent=Msa1120000-mRNA-1
chr7_3 CDS 69166559 69166605 69166559 ID=Msa1120000-mRNA-1:cds;Parent=Msa1120000-mRNA-1
chr7_3 CDS 69166392 69166468 69166392 ID=Msa1120000-mRNA-1:cds;Parent=Msa1120000-mRNA-1
chr7_3 CDS 69166000 69166281 69166000 ID=Msa1120000-mRNA-1:cds;Parent=Msa1120000-mRNA-1
Gene Sequence

>Msa1120000

ATGATGATGGATGAGGGAGAAGGTAAGAAGAAAGTTGTGGTTCAGAAAACCGAGGCGTGTGGTTTCATGGCAGGTGTTGAAGATGAACTAGGGTTTGTCAACGTGAAAGGAGACAACAACAACAACAAGATTAACGGGTCGGGTCAACGGATCCATCATGATCATGGTTTAGTTGCTGCTGCATTTGGAACTGTTCATAGGAAGAAAAGGATGGCTAGGCAAAGAAGATCTTCATCTTCTACCATCACTATTCATCTCAAAAATCTTCCTTCTTCTACTACTACTACTACTACCTCGCACGTGCCAATCTCTCCAATTCCACCTCTTTTTCATTCTCTCCCTCCCGCACGTGAAATCGATCATAGGAGGTTGAGATTCCTTTTCCAAAAGGAGTTGAAAAACAGTGATGTTAGCTCCCTTAGGAGAATGGTGTTGCCTAAGAAAGCAGCAGAGGCTTTCCTTCCTGTCCTTGAGTCAAAGGAAGGAATTCTTCTCAGCATGAATGATATAGATGGTCTTCATGTATGGAGTTTCAAGTACAGGTTTTGGCCTAACAATAACAGTCGGATGTATGTACTTGAAAACACTGGAGACTTTGTTAGCACGCATGGTCTACGCTTTGGAGACTCCATTATGGTTTACCAAGATAATCACAATCACAATTATGTAATTCAGGCAAAGAAAGCTTGTGATCAAGATGAGTATATGGAAGAAGCTAATGATACAATCAATCATATCTTTGTTGATGATTATGAGGTGAACAAAAGTTGCTTTGATGTAGCTTATCCTGCAATGAATGATACAAGCATGTCATTTATATATGACACAACCATCTCAAATGATTCACCACTTGATTTTTTGGGTGGATCAATGACAAACTACTCAAGAATTGGATCAGTTGAAACATTTGGCTCTGTTGAGAATCTGTCACTTGATGACTTCTATTGA
Protein sequence

>Msa1120000

MMMDEGEGKKKVVVQKTEACGFMAGVEDELGFVNVKGDNNNNKINGSGQRIHHDHGLVAAAFGTVHRKKRMARQRRSSSSTITIHLKNLPSSTTTTTTSHVPISPIPPLFHSLPPAREIDHRRLRFLFQKELKNSDVSSLRRMVLPKKAAEAFLPVLESKEGILLSMNDIDGLHVWSFKYRFWPNNNSRMYVLENTGDFVSTHGLRFGDSIMVYQDNHNHNYVIQAKKACDQDEYMEEANDTINHIFVDDYEVNKSCFDVAYPAMNDTSMSFIYDTTISNDSPLDFLGGSMTNYSRIGSVETFGSVENLSLDDFY*