AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1120190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1120190 MtrunA17_Chr7g0251661 79.114 158 33 0 1 158 1 158 2.64e-95 273
Msa1120190 MtrunA17_Chr7g0251671 71.975 157 44 0 1 157 1 157 4.01e-81 237
Msa1120190 MtrunA17_Chr7g0251651 64.246 179 56 3 8 178 16 194 1.93e-80 237
Msa1120190 MtrunA17_Chr3g0124761 34.868 152 89 5 6 154 3 147 4.64e-22 90.1
Msa1120190 MtrunA17_Chr3g0139331 35.338 133 80 5 7 135 8 138 2.28e-21 89.0
Msa1120190 MtrunA17_Chr6g0461661 37.692 130 75 4 8 133 16 143 3.91e-21 89.0
Msa1120190 MtrunA17_Chr7g0253011 36.364 132 76 5 8 133 15 144 2.47e-20 86.7
Msa1120190 MtrunA17_Chr4g0042561 32.051 156 96 6 3 154 2 151 8.59e-20 84.0
Msa1120190 MtrunA17_Chr7g0252971 31.544 149 89 4 7 144 5 151 9.64e-20 84.7
Msa1120190 MtrunA17_Chr1g0147421 35.821 134 79 5 7 135 24 155 7.15e-19 82.4
Msa1120190 MtrunA17_Chr8g0359921 31.206 141 88 5 6 143 10 144 7.35e-19 82.0
Msa1120190 MtrunA17_Chr2g0318101 34.109 129 79 4 9 133 14 140 9.74e-19 82.0
Msa1120190 MtrunA17_Chr3g0130731 33.088 136 84 4 6 138 3 134 1.14e-18 81.3
Msa1120190 MtrunA17_Chr4g0047841 34.375 128 78 4 10 133 15 140 1.18e-18 81.6
Msa1120190 MtrunA17_Chr2g0318081 31.206 141 88 5 6 143 12 146 1.30e-18 81.6
Msa1120190 MtrunA17_Chr6g0484441 35.385 130 78 5 7 133 2 128 1.94e-18 80.9
Msa1120190 MtrunA17_Chr2g0309891 28.804 184 108 5 6 168 2 183 2.24e-18 80.9
Msa1120190 MtrunA17_Chr7g0214071 33.824 136 81 6 2 133 7 137 2.66e-18 79.7
Msa1120190 MtrunA17_Chr3g0144271 37.500 136 78 6 3 133 16 149 3.07e-18 80.9
Msa1120190 MtrunA17_Chr6g0453451 33.333 135 84 4 2 133 12 143 3.61e-18 80.1
Msa1120190 MtrunA17_Chr8g0384721 32.090 134 84 4 8 138 5 134 4.04e-18 79.3
Msa1120190 MtrunA17_Chr5g0433781 33.588 131 83 3 6 133 2 131 8.68e-18 80.1
Msa1120190 MtrunA17_Chr2g0316971 33.594 128 79 5 10 133 23 148 1.40e-17 79.0
Msa1120190 MtrunA17_Chr7g0217711 33.117 154 97 5 5 155 14 164 1.41e-17 75.9
Msa1120190 MtrunA17_Chr5g0417791 31.126 151 94 6 8 154 1 145 1.59e-17 77.4
Msa1120190 MtrunA17_Chr5g0417001 32.374 139 87 5 7 141 12 147 3.25e-17 78.2
Msa1120190 MtrunA17_Chr3g0109821 32.824 131 84 3 6 133 2 131 6.15e-17 77.8
Msa1120190 MtrunA17_Chr3g0091921 30.882 136 88 5 1 133 7 139 1.08e-16 75.9
Msa1120190 MtrunA17_Chr2g0309331 31.387 137 90 3 1 133 1 137 1.26e-16 75.9
Msa1120190 MtrunA17_Chr4g0060931 32.308 130 82 5 7 133 14 140 1.32e-16 76.3
Msa1120190 MtrunA17_Chr6g0481091 33.333 129 82 3 8 133 4 131 1.57e-16 74.3
Msa1120190 MtrunA17_Chr2g0311651 33.571 140 83 6 1 133 14 150 2.95e-16 74.7
Msa1120190 MtrunA17_Chr6g0453131 31.206 141 92 3 3 140 8 146 3.23e-16 75.1
Msa1120190 MtrunA17_Chr2g0326361 31.333 150 93 6 10 154 14 158 4.43e-16 74.3
Msa1120190 MtrunA17_Chr5g0442771 34.091 132 79 5 7 133 16 144 4.46e-16 74.3
Msa1120190 MtrunA17_Chr7g0217721 34.091 132 81 5 5 133 14 142 4.65e-16 74.7
Msa1120190 MtrunA17_Chr8g0385991 31.250 128 80 4 10 133 14 137 6.83e-16 72.4
Msa1120190 MtrunA17_Chr8g0361731 31.469 143 91 5 2 140 7 146 9.03e-16 73.9
Msa1120190 MtrunA17_Chr1g0197351 32.283 127 82 3 10 133 42 167 1.44e-15 73.6
Msa1120190 MtrunA17_Chr2g0311661 35.338 133 76 6 9 133 19 149 2.02e-15 72.4
Msa1120190 MtrunA17_Chr7g0263931 30.827 133 86 4 5 133 6 136 2.84e-15 72.0
Msa1120190 MtrunA17_Chr1g0201021 31.746 126 82 3 13 135 22 146 5.55e-15 72.0
Msa1120190 MtrunA17_Chr3g0130101 33.083 133 84 4 4 133 2 132 5.76e-15 71.6
Msa1120190 MtrunA17_Chr4g0017921 29.630 189 110 8 1 172 1 183 2.16e-14 69.7
Msa1120190 MtrunA17_Chr1g0183741 29.375 160 100 6 10 162 10 163 2.43e-14 68.9
Msa1120190 MtrunA17_Chr5g0405211 32.031 128 82 4 10 133 8 134 2.70e-14 69.7
Msa1120190 MtrunA17_Chr4g0018391 31.250 128 83 4 10 133 7 133 2.88e-14 69.3
Msa1120190 MtrunA17_Chr5g0428991 34.646 127 79 3 10 133 25 150 5.00e-14 69.3
Msa1120190 MtrunA17_Chr7g0261411 32.812 128 80 4 10 133 10 135 6.05e-14 68.2
Msa1120190 MtrunA17_Chr8g0345671 30.070 143 94 4 9 146 10 151 6.41e-14 68.6
Msa1120190 MtrunA17_Chr8g0372121 31.250 128 83 4 10 133 6 132 6.57e-14 68.2
Msa1120190 MtrunA17_Chr5g0398971 29.688 128 86 3 10 133 7 134 1.02e-13 67.8
Msa1120190 MtrunA17_Chr4g0076731 29.839 124 82 3 13 133 57 178 1.35e-13 67.8
Msa1120190 MtrunA17_Chr3g0128151 33.071 127 81 3 10 133 22 147 1.48e-13 67.8
Msa1120190 MtrunA17_Chr2g0312321 29.078 141 94 4 6 144 7 143 1.68e-13 67.0
Msa1120190 MtrunA17_Chr8g0345701 26.573 143 83 5 10 133 7 146 3.31e-13 64.3
Msa1120190 MtrunA17_Chr1g0197361 31.343 134 84 5 13 142 11 140 4.45e-13 66.2
Msa1120190 MtrunA17_Chr8g0364791 30.709 127 84 3 10 133 6 131 5.09e-13 66.2
Msa1120190 MtrunA17_Chr1g0200561 29.688 128 85 4 10 133 19 145 5.38e-13 65.9
Msa1120190 MtrunA17_Chr8g0345711 30.882 136 88 5 10 140 12 146 7.42e-13 63.5
Msa1120190 MtrunA17_Chr8g0345691 29.452 146 89 5 10 146 12 152 3.03e-12 63.9
Msa1120190 MtrunA17_Chr2g0321631 31.061 132 84 3 9 133 3 134 4.95e-12 62.4
Msa1120190 MtrunA17_Chr3g0092671 28.000 125 85 3 13 133 8 131 2.41e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1120190 AT5G13180.1 38.346 133 73 5 5 133 9 136 2.14e-23 93.2
Msa1120190 AT5G18270.1 37.209 129 76 4 8 133 19 145 1.12e-21 90.1
Msa1120190 AT5G18270.2 36.434 129 77 4 8 133 19 145 2.04e-21 89.4
Msa1120190 AT1G77450.1 33.758 157 94 5 1 154 1 150 2.67e-21 87.8
Msa1120190 AT3G10500.1 35.036 137 84 4 10 142 9 144 5.42e-21 89.7
Msa1120190 AT5G04410.1 35.433 127 78 3 10 133 9 134 7.16e-21 89.4
Msa1120190 AT5G63790.2 33.824 136 83 4 6 138 56 187 2.51e-20 86.3
Msa1120190 AT5G63790.1 33.824 136 83 4 6 138 46 177 2.58e-20 86.3
Msa1120190 AT1G76420.1 36.029 136 81 5 2 133 14 147 2.60e-20 86.7
Msa1120190 AT5G08790.1 33.088 136 84 4 6 138 3 134 3.05e-20 85.5
Msa1120190 AT3G04070.2 30.464 151 96 4 1 144 1 149 8.00e-20 85.1
Msa1120190 AT2G24430.2 35.878 131 78 5 7 133 13 141 9.02e-20 84.7
Msa1120190 AT2G24430.1 35.878 131 78 5 7 133 13 141 9.02e-20 84.7
Msa1120190 AT3G04070.1 30.464 151 96 4 1 144 1 149 1.34e-19 84.7
Msa1120190 AT1G61110.1 33.858 127 79 3 10 133 16 140 4.90e-19 82.8
Msa1120190 AT3G29035.1 34.074 135 83 5 2 133 16 147 8.06e-19 82.4
Msa1120190 AT5G53950.1 31.013 158 84 6 10 144 17 172 2.18e-18 81.6
Msa1120190 AT1G01720.1 32.836 134 83 4 8 138 5 134 2.21e-18 80.5
Msa1120190 AT5G39610.1 33.824 136 84 5 1 133 11 143 2.93e-18 80.1
Msa1120190 AT5G61430.1 30.857 175 88 7 7 151 13 184 3.34e-18 80.9
Msa1120190 AT5G07680.1 33.582 134 83 5 3 133 10 140 3.48e-18 80.5
Msa1120190 AT3G15500.1 31.206 141 88 5 6 143 10 144 3.69e-18 80.5
Msa1120190 AT3G10490.1 34.586 133 83 4 4 133 21 152 9.55e-18 78.2
Msa1120190 AT1G52890.1 31.206 141 88 5 6 143 10 144 1.02e-17 79.3
Msa1120190 AT4G27410.2 31.298 131 84 4 6 133 10 137 1.07e-17 79.0
Msa1120190 AT5G07680.2 34.109 129 79 5 8 133 1 126 1.65e-17 78.6
Msa1120190 AT3G04060.1 33.333 129 81 4 8 133 18 144 2.24e-17 78.6
Msa1120190 AT2G33480.2 34.615 130 74 5 8 133 13 135 2.33e-17 77.4
Msa1120190 AT5G17260.1 32.824 131 84 3 6 133 2 131 2.36e-17 79.0
Msa1120190 AT2G33480.1 34.615 130 74 5 8 133 13 135 2.37e-17 77.4
Msa1120190 AT3G10480.1 35.433 127 78 4 10 133 27 152 5.27e-17 78.2
Msa1120190 AT3G10480.2 35.433 127 78 4 10 133 27 152 5.52e-17 77.8
Msa1120190 AT3G03200.1 33.588 131 83 3 6 133 2 131 5.99e-17 77.8
Msa1120190 AT3G15170.1 32.812 128 80 5 10 133 20 145 6.21e-17 77.0
Msa1120190 AT1G69490.1 31.343 134 86 4 3 133 2 132 6.27e-17 76.3
Msa1120190 AT3G10490.2 34.586 133 83 4 4 133 21 152 7.75e-17 77.4
Msa1120190 AT3G17730.1 33.588 131 83 3 6 133 2 131 7.78e-17 75.9
Msa1120190 AT1G65910.1 32.061 131 85 3 6 133 2 131 8.07e-17 77.8
Msa1120190 AT3G15510.1 33.065 124 77 4 14 133 21 142 9.87e-17 77.0
Msa1120190 AT3G18400.1 31.056 161 103 6 7 162 2 159 1.38e-16 75.9
Msa1120190 AT1G54330.1 32.824 131 84 3 6 133 2 131 2.35e-16 75.1
Msa1120190 AT1G34180.1 31.746 126 82 3 13 135 19 143 5.30e-16 75.1
Msa1120190 AT3G12910.1 35.252 139 79 5 2 133 14 148 5.63e-16 74.3
Msa1120190 AT2G43000.1 33.582 134 82 5 3 133 12 141 5.63e-16 73.9
Msa1120190 AT5G39820.1 29.545 132 87 4 13 140 23 152 6.10e-16 74.3
Msa1120190 AT1G34180.3 31.746 126 82 3 13 135 19 143 8.31e-16 74.7
Msa1120190 AT4G36160.2 30.657 137 90 4 1 133 5 140 9.03e-16 74.3
Msa1120190 AT4G36160.1 30.657 137 90 4 1 133 1 136 1.00e-15 73.9
Msa1120190 AT4G36160.3 30.657 137 90 4 1 133 1 136 1.00e-15 73.9
Msa1120190 AT1G34190.1 31.387 137 85 4 13 141 19 154 1.17e-15 74.3
Msa1120190 AT1G26870.1 31.008 129 81 5 10 133 16 141 1.74e-15 73.6
Msa1120190 AT4G27410.3 28.767 146 85 5 6 133 10 154 3.66e-15 72.0
Msa1120190 AT5G66300.1 30.882 136 89 4 2 133 4 138 4.43e-15 71.6
Msa1120190 AT4G17980.1 28.986 138 94 3 10 143 6 143 9.95e-15 70.5
Msa1120190 AT2G27300.1 34.400 125 79 3 13 135 17 140 1.07e-14 70.9
Msa1120190 AT1G33060.1 33.858 127 80 4 10 133 24 149 1.07e-14 71.6
Msa1120190 AT1G33060.2 33.858 127 80 4 10 133 24 149 1.13e-14 71.6
Msa1120190 AT5G62380.2 29.771 131 81 5 10 133 7 133 1.79e-14 70.5
Msa1120190 AT5G62380.1 29.771 131 81 5 10 133 7 133 1.79e-14 70.5
Msa1120190 AT4G17980.2 28.986 138 94 3 10 143 6 143 2.51e-14 69.7
Msa1120190 AT4G35580.1 33.071 127 81 3 10 133 9 134 4.73e-14 69.7
Msa1120190 AT1G79580.3 29.104 134 91 3 4 133 11 144 5.01e-14 69.3
Msa1120190 AT1G79580.5 29.104 134 91 3 4 133 11 144 5.01e-14 69.3
Msa1120190 AT1G79580.4 29.104 134 91 3 4 133 11 144 5.01e-14 69.3
Msa1120190 AT1G79580.2 29.104 134 91 3 4 133 11 144 5.01e-14 69.3
Msa1120190 AT1G79580.1 29.104 134 91 3 4 133 11 144 5.01e-14 69.3
Msa1120190 AT5G46590.1 29.134 127 87 2 10 133 6 132 5.76e-14 68.6
Msa1120190 AT5G46590.2 29.134 127 87 2 10 133 6 132 5.80e-14 68.6
Msa1120190 AT4G35580.2 33.071 127 81 3 10 133 9 134 6.26e-14 69.3
Msa1120190 AT4G35580.3 33.071 127 81 3 10 133 9 134 6.32e-14 69.3
Msa1120190 AT1G34180.2 29.710 138 81 4 13 135 19 155 6.44e-14 69.3
Msa1120190 AT2G18060.3 31.250 128 83 4 10 133 9 135 6.45e-14 68.9
Msa1120190 AT2G18060.1 31.250 128 83 4 10 133 9 135 6.45e-14 68.9
Msa1120190 AT1G34180.4 29.710 138 81 4 13 135 19 155 1.06e-13 68.6
Msa1120190 AT1G33280.1 31.655 139 88 5 1 135 1 136 1.12e-13 67.8
Msa1120190 AT4G10350.1 32.353 136 87 4 10 141 9 143 1.35e-13 67.8
Msa1120190 AT5G24590.2 31.496 127 84 2 10 133 13 139 2.79e-13 67.4
Msa1120190 AT3G12977.1 30.147 136 85 5 1 133 13 141 4.48e-13 65.9
Msa1120190 AT2G02450.1 28.226 124 84 3 13 133 54 175 8.26e-13 65.9
Msa1120190 AT1G71930.2 29.688 128 85 4 10 133 9 135 1.49e-12 64.7
Msa1120190 AT1G71930.1 29.688 128 85 4 10 133 9 135 1.49e-12 64.7
Msa1120190 AT2G02450.2 28.226 124 84 3 13 133 54 175 1.55e-12 65.1
Msa1120190 AT5G64060.1 29.771 131 88 4 6 133 2 131 1.59e-12 65.1
Msa1120190 AT5G04395.1 28.758 153 93 5 1 137 19 171 1.75e-12 63.2
Msa1120190 AT5G14000.2 27.206 136 93 4 4 136 9 141 2.47e-12 62.0
Msa1120190 AT1G32870.1 30.709 127 86 2 10 135 10 135 4.99e-12 63.9
Msa1120190 AT1G32870.3 30.709 127 86 2 10 135 46 171 5.69e-12 63.5
Msa1120190 AT1G32870.2 30.709 127 86 2 10 135 10 135 5.85e-12 63.5
Msa1120190 AT1G02230.1 26.708 161 108 4 11 164 4 161 1.01e-11 62.8
Msa1120190 AT5G14000.1 27.206 136 93 4 4 136 9 141 1.34e-11 60.8
Msa1120190 AT3G49530.2 28.346 127 88 2 10 133 13 139 1.58e-11 62.0
Msa1120190 AT3G49530.1 28.346 127 88 2 10 133 13 139 1.94e-11 62.0
Msa1120190 AT3G44350.1 28.244 131 88 3 9 133 4 134 6.41e-11 58.2
Msa1120190 AT5G64530.2 29.231 130 78 5 8 135 1 118 6.86e-11 58.2
Msa1120190 AT2G46770.1 25.676 148 104 5 4 146 10 156 9.42e-11 59.7

Find 47 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATCTACCTGCTGGATTC+AGG 0.237662 7_3:+69418185 Msa1120190:CDS
GAACAGTCGGTTCGAAAATA+TGG 0.258410 7_3:+69418694 Msa1120190:CDS
GAAAGAGCACATTTGTTTAC+TGG 0.275802 7_3:+69418801 Msa1120190:CDS
TGCAGGTAATAGGTACTCTT+TGG 0.340271 7_3:+69418640 Msa1120190:intron
TCAAATTCAGACATGACATT+TGG 0.359524 7_3:-69418274 None:intergenic
ATTGTATTCCTTACCCTAGT+TGG 0.360335 7_3:-69418896 None:intergenic
AGTATTATTTCTTTGATCTC+AGG 0.394952 7_3:+69418672 Msa1120190:CDS
AATATGGACGCAAGACGAGC+TGG 0.462643 7_3:+69418710 Msa1120190:CDS
GACGCAAGACGAGCTGGAAA+TGG 0.464716 7_3:+69418716 Msa1120190:CDS
GCACATTTGTTTACTGGAGT+GGG 0.466777 7_3:+69418807 Msa1120190:CDS
TGAGTTCATGGAGAGGGAGG+AGG 0.476558 7_3:-69419143 None:intergenic
TCAGACATGACATTTGGAAA+AGG 0.488819 7_3:-69418268 None:intergenic
GAAGAAGGCCACCACACTAA+TGG 0.491907 7_3:+69419100 Msa1120190:CDS
ACTGAGCCATGGAGGCTGCC+AGG 0.492712 7_3:+69418307 Msa1120190:CDS
ACATGACATTTGGAAAAGGT+TGG 0.502196 7_3:-69418264 None:intergenic
AAACCTCCTGGCAGCCTCCA+TGG 0.506870 7_3:-69418313 None:intergenic
AGCACATTTGTTTACTGGAG+TGG 0.509000 7_3:+69418806 Msa1120190:CDS
TGTCAGCTGTTGGTGCCTAT+CGG 0.513489 7_3:+69419047 Msa1120190:intron
AAATTCATGCATCCACCATC+GGG 0.513722 7_3:-69418852 None:intergenic
TTTGTTTACTGGAGTGGGCA+AGG 0.516244 7_3:+69418812 Msa1120190:CDS
GAGTTCATGGAGAGGGAGGA+GGG 0.516664 7_3:-69419142 None:intergenic
CAAATTCATGCATCCACCAT+CGG 0.517794 7_3:-69418853 None:intergenic
GCTGAGCTTGATCTACCTGC+TGG 0.518168 7_3:+69418178 Msa1120190:CDS
GACGAGCTGGAAATGGTGAA+TGG 0.521079 7_3:+69418723 Msa1120190:CDS
ATCGGATCTTTAAGATATGA+AGG 0.521757 7_3:+69419065 Msa1120190:CDS
ATAGGTACTCTTTGGAGCAT+AGG 0.529909 7_3:+69418648 Msa1120190:CDS
AGGTGGCAAAACTGAGAAGA+AGG 0.540768 7_3:+69419085 Msa1120190:CDS
AGTTCATGGAGAGGGAGGAG+GGG 0.541559 7_3:-69419141 None:intergenic
ATATATTTCAAACTGAGCCA+TGG 0.550284 7_3:+69418296 Msa1120190:CDS
TGCATCCTCCATTAGTGTGG+TGG 0.554338 7_3:-69419108 None:intergenic
TGAAACATGAGTTCATGGAG+AGG 0.563520 7_3:-69419150 None:intergenic
GAGAGTAGTTGAAAAGAACA+AGG 0.579351 7_3:+69418745 Msa1120190:CDS
TCTTTGATCTCAGGAACAGT+CGG 0.584323 7_3:+69418681 Msa1120190:CDS
GGATCAAACCTGAATCCAGC+AGG 0.586690 7_3:-69418193 None:intergenic
ATCGAGCTCTGATGACCCGA+TGG 0.606095 7_3:+69418837 Msa1120190:CDS
ACATGAGTTCATGGAGAGGG+AGG 0.614894 7_3:-69419146 None:intergenic
GAAACATGAGTTCATGGAGA+GGG 0.632009 7_3:-69419149 None:intergenic
TCAACAAGTTGACGATCGGT+CGG 0.636753 7_3:-69418214 None:intergenic
GAAGGCCACCACACTAATGG+AGG 0.652157 7_3:+69419103 Msa1120190:CDS
TCATATCTTAAAGATCCGAT+AGG 0.652337 7_3:-69419062 None:intergenic
GAGCTCTGATGACCCGATGG+TGG 0.653753 7_3:+69418840 Msa1120190:CDS
GCACTCAACAAGTTGACGAT+CGG 0.655422 7_3:-69418218 None:intergenic
TATTTCAAACTGAGCCATGG+AGG 0.675314 7_3:+69418299 Msa1120190:CDS
ACTTGCATCCTCCATTAGTG+TGG 0.678198 7_3:-69419111 None:intergenic
GAGCCATGGAGGCTGCCAGG+AGG 0.693460 7_3:+69418310 Msa1120190:CDS
TGGATGCATGAATTTGTCAT+CGG 0.708626 7_3:+69418860 Msa1120190:CDS
GGATCTTTAAGATATGAAGG+TGG 0.724453 7_3:+69419068 Msa1120190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATGATATTTAATTTTATG+TGG + chr7_3:69418552-69418571 Msa1120190:intron 10.0%
!!! TATGATATTTAATTTTATGT+GGG + chr7_3:69418553-69418572 Msa1120190:intron 10.0%
!!! ATATGATATTTAATTTTATG+TGG + chr7_3:69418552-69418571 Msa1120190:intron 10.0%
!!! TATGATATTTAATTTTATGT+GGG + chr7_3:69418553-69418572 Msa1120190:intron 10.0%
!!! AAATTTTTTGATATTGATAG+TGG - chr7_3:69418927-69418946 None:intergenic 15.0%
!! TATCAATATCAAAAAATTTG+AGG + chr7_3:69418928-69418947 Msa1120190:intron 15.0%
!!! AAATTTTTTGATATTGATAG+TGG - chr7_3:69418927-69418946 None:intergenic 15.0%
!! TATCAATATCAAAAAATTTG+AGG + chr7_3:69418928-69418947 Msa1120190:intron 15.0%
!! TCATAAAGAAAGAGAATAAA+GGG - chr7_3:69418380-69418399 None:intergenic 20.0%
!! TATTGATAAAACATGTACAA+TGG - chr7_3:69418403-69418422 None:intergenic 20.0%
!! ATATCATATAAGCATTTCAT+GGG - chr7_3:69418541-69418560 None:intergenic 20.0%
!! AATATCATATAAGCATTTCA+TGG - chr7_3:69418542-69418561 None:intergenic 20.0%
!!! AATTTGTTTTTTTATGTTGC+AGG + chr7_3:69418623-69418642 Msa1120190:intron 20.0%
!!! TTTTGAACTCAAATTAATGT+AGG + chr7_3:69419024-69419043 Msa1120190:intron 20.0%
!! TCATAAAGAAAGAGAATAAA+GGG - chr7_3:69418380-69418399 None:intergenic 20.0%
!! TATTGATAAAACATGTACAA+TGG - chr7_3:69418403-69418422 None:intergenic 20.0%
!! ATATCATATAAGCATTTCAT+GGG - chr7_3:69418541-69418560 None:intergenic 20.0%
!! AATATCATATAAGCATTTCA+TGG - chr7_3:69418542-69418561 None:intergenic 20.0%
!!! AATTTGTTTTTTTATGTTGC+AGG + chr7_3:69418623-69418642 Msa1120190:intron 20.0%
!!! TTTTGAACTCAAATTAATGT+AGG + chr7_3:69419024-69419043 Msa1120190:intron 20.0%
! GTCATAAAGAAAGAGAATAA+AGG - chr7_3:69418381-69418400 None:intergenic 25.0%
!! ATTTTCTTAGTGTTCTTTGA+TGG + chr7_3:69418431-69418450 Msa1120190:intron 25.0%
!!! TTTTTTATGTTGCAGGTAAT+AGG + chr7_3:69418630-69418649 Msa1120190:intron 25.0%
!! AGTATTATTTCTTTGATCTC+AGG + chr7_3:69418672-69418691 Msa1120190:CDS 25.0%
!! TAGAATTTTTGATCCAACTA+GGG + chr7_3:69418883-69418902 Msa1120190:CDS 25.0%
!!! TGCTAGTTTTATGTAAACTT+TGG + chr7_3:69418971-69418990 Msa1120190:intron 25.0%
! GTCATAAAGAAAGAGAATAA+AGG - chr7_3:69418381-69418400 None:intergenic 25.0%
!! ATTTTCTTAGTGTTCTTTGA+TGG + chr7_3:69418431-69418450 Msa1120190:intron 25.0%
!!! TTTTTTATGTTGCAGGTAAT+AGG + chr7_3:69418630-69418649 Msa1120190:intron 25.0%
!! AGTATTATTTCTTTGATCTC+AGG + chr7_3:69418672-69418691 Msa1120190:CDS 25.0%
!! TAGAATTTTTGATCCAACTA+GGG + chr7_3:69418883-69418902 Msa1120190:CDS 25.0%
!!! TGCTAGTTTTATGTAAACTT+TGG + chr7_3:69418971-69418990 Msa1120190:intron 25.0%
TCAAATTCAGACATGACATT+TGG - chr7_3:69418277-69418296 None:intergenic 30.0%
ATATATTTCAAACTGAGCCA+TGG + chr7_3:69418296-69418315 Msa1120190:CDS 30.0%
! GTAGAATTTTTGATCCAACT+AGG + chr7_3:69418882-69418901 Msa1120190:CDS 30.0%
TCATATCTTAAAGATCCGAT+AGG - chr7_3:69419065-69419084 None:intergenic 30.0%
!! ATCGGATCTTTAAGATATGA+AGG + chr7_3:69419065-69419084 Msa1120190:CDS 30.0%
TCAAATTCAGACATGACATT+TGG - chr7_3:69418277-69418296 None:intergenic 30.0%
ATATATTTCAAACTGAGCCA+TGG + chr7_3:69418296-69418315 Msa1120190:CDS 30.0%
! GTAGAATTTTTGATCCAACT+AGG + chr7_3:69418882-69418901 Msa1120190:CDS 30.0%
TCATATCTTAAAGATCCGAT+AGG - chr7_3:69419065-69419084 None:intergenic 30.0%
!! ATCGGATCTTTAAGATATGA+AGG + chr7_3:69419065-69419084 Msa1120190:CDS 30.0%
ACATGACATTTGGAAAAGGT+TGG - chr7_3:69418267-69418286 None:intergenic 35.0%
TCAGACATGACATTTGGAAA+AGG - chr7_3:69418271-69418290 None:intergenic 35.0%
TTGTGTCTTAGACATGCAAT+TGG + chr7_3:69418480-69418499 Msa1120190:intron 35.0%
GAGAGTAGTTGAAAAGAACA+AGG + chr7_3:69418745-69418764 Msa1120190:CDS 35.0%
! GAAAGAGCACATTTGTTTAC+TGG + chr7_3:69418801-69418820 Msa1120190:CDS 35.0%
TGGATGCATGAATTTGTCAT+CGG + chr7_3:69418860-69418879 Msa1120190:CDS 35.0%
! TTTTTGATCCAACTAGGGTA+AGG + chr7_3:69418888-69418907 Msa1120190:intron 35.0%
ATTGTATTCCTTACCCTAGT+TGG - chr7_3:69418899-69418918 None:intergenic 35.0%
!! GGATCTTTAAGATATGAAGG+TGG + chr7_3:69419068-69419087 Msa1120190:CDS 35.0%
ACATGACATTTGGAAAAGGT+TGG - chr7_3:69418267-69418286 None:intergenic 35.0%
TCAGACATGACATTTGGAAA+AGG - chr7_3:69418271-69418290 None:intergenic 35.0%
TTGTGTCTTAGACATGCAAT+TGG + chr7_3:69418480-69418499 Msa1120190:intron 35.0%
GAGAGTAGTTGAAAAGAACA+AGG + chr7_3:69418745-69418764 Msa1120190:CDS 35.0%
! GAAAGAGCACATTTGTTTAC+TGG + chr7_3:69418801-69418820 Msa1120190:CDS 35.0%
TGGATGCATGAATTTGTCAT+CGG + chr7_3:69418860-69418879 Msa1120190:CDS 35.0%
! TTTTTGATCCAACTAGGGTA+AGG + chr7_3:69418888-69418907 Msa1120190:intron 35.0%
ATTGTATTCCTTACCCTAGT+TGG - chr7_3:69418899-69418918 None:intergenic 35.0%
!! GGATCTTTAAGATATGAAGG+TGG + chr7_3:69419068-69419087 Msa1120190:CDS 35.0%
TATTTCAAACTGAGCCATGG+AGG + chr7_3:69418299-69418318 Msa1120190:CDS 40.0%
! GTTTTCTTTCACAAACCTCC+TGG - chr7_3:69418328-69418347 None:intergenic 40.0%
TGTCTTAGACATGCAATTGG+AGG + chr7_3:69418483-69418502 Msa1120190:intron 40.0%
GTCTTAGACATGCAATTGGA+GGG + chr7_3:69418484-69418503 Msa1120190:intron 40.0%
TCTTAGACATGCAATTGGAG+GGG + chr7_3:69418485-69418504 Msa1120190:intron 40.0%
TGCAGGTAATAGGTACTCTT+TGG + chr7_3:69418640-69418659 Msa1120190:intron 40.0%
!! ATAGGTACTCTTTGGAGCAT+AGG + chr7_3:69418648-69418667 Msa1120190:CDS 40.0%
TCTTTGATCTCAGGAACAGT+CGG + chr7_3:69418681-69418700 Msa1120190:CDS 40.0%
!! GAACAGTCGGTTCGAAAATA+TGG + chr7_3:69418694-69418713 Msa1120190:CDS 40.0%
AGCACATTTGTTTACTGGAG+TGG + chr7_3:69418806-69418825 Msa1120190:CDS 40.0%
GCACATTTGTTTACTGGAGT+GGG + chr7_3:69418807-69418826 Msa1120190:CDS 40.0%
AAATTCATGCATCCACCATC+GGG - chr7_3:69418855-69418874 None:intergenic 40.0%
CAAATTCATGCATCCACCAT+CGG - chr7_3:69418856-69418875 None:intergenic 40.0%
TTAATGTAGGTGTCAGCTGT+TGG + chr7_3:69419037-69419056 Msa1120190:intron 40.0%
TATTTCAAACTGAGCCATGG+AGG + chr7_3:69418299-69418318 Msa1120190:CDS 40.0%
! GTTTTCTTTCACAAACCTCC+TGG - chr7_3:69418328-69418347 None:intergenic 40.0%
TGTCTTAGACATGCAATTGG+AGG + chr7_3:69418483-69418502 Msa1120190:intron 40.0%
GTCTTAGACATGCAATTGGA+GGG + chr7_3:69418484-69418503 Msa1120190:intron 40.0%
TCTTAGACATGCAATTGGAG+GGG + chr7_3:69418485-69418504 Msa1120190:intron 40.0%
TGCAGGTAATAGGTACTCTT+TGG + chr7_3:69418640-69418659 Msa1120190:intron 40.0%
!! ATAGGTACTCTTTGGAGCAT+AGG + chr7_3:69418648-69418667 Msa1120190:CDS 40.0%
TCTTTGATCTCAGGAACAGT+CGG + chr7_3:69418681-69418700 Msa1120190:CDS 40.0%
!! GAACAGTCGGTTCGAAAATA+TGG + chr7_3:69418694-69418713 Msa1120190:CDS 40.0%
AGCACATTTGTTTACTGGAG+TGG + chr7_3:69418806-69418825 Msa1120190:CDS 40.0%
GCACATTTGTTTACTGGAGT+GGG + chr7_3:69418807-69418826 Msa1120190:CDS 40.0%
AAATTCATGCATCCACCATC+GGG - chr7_3:69418855-69418874 None:intergenic 40.0%
CAAATTCATGCATCCACCAT+CGG - chr7_3:69418856-69418875 None:intergenic 40.0%
TTAATGTAGGTGTCAGCTGT+TGG + chr7_3:69419037-69419056 Msa1120190:intron 40.0%
TTGATCTACCTGCTGGATTC+AGG + chr7_3:69418185-69418204 Msa1120190:CDS 45.0%
! TCAACAAGTTGACGATCGGT+CGG - chr7_3:69418217-69418236 None:intergenic 45.0%
! GCACTCAACAAGTTGACGAT+CGG - chr7_3:69418221-69418240 None:intergenic 45.0%
! GGTTGCAGATTTTCTTCGTC+AGG - chr7_3:69418246-69418265 None:intergenic 45.0%
TTTGTTTACTGGAGTGGGCA+AGG + chr7_3:69418812-69418831 Msa1120190:CDS 45.0%
AGGTGGCAAAACTGAGAAGA+AGG + chr7_3:69419085-69419104 Msa1120190:CDS 45.0%
ACTTGCATCCTCCATTAGTG+TGG - chr7_3:69419114-69419133 None:intergenic 45.0%
TTGATCTACCTGCTGGATTC+AGG + chr7_3:69418185-69418204 Msa1120190:CDS 45.0%
! TCAACAAGTTGACGATCGGT+CGG - chr7_3:69418217-69418236 None:intergenic 45.0%
! GCACTCAACAAGTTGACGAT+CGG - chr7_3:69418221-69418240 None:intergenic 45.0%
! GGTTGCAGATTTTCTTCGTC+AGG - chr7_3:69418246-69418265 None:intergenic 45.0%
TTTGTTTACTGGAGTGGGCA+AGG + chr7_3:69418812-69418831 Msa1120190:CDS 45.0%
AGGTGGCAAAACTGAGAAGA+AGG + chr7_3:69419085-69419104 Msa1120190:CDS 45.0%
ACTTGCATCCTCCATTAGTG+TGG - chr7_3:69419114-69419133 None:intergenic 45.0%
!!! AAAATTTAATTTATTCATTT+TGG + chr7_3:69418592-69418611 Msa1120190:intron 5.0%
!!! AAATTTAATTTATTCATTTT+GGG + chr7_3:69418593-69418612 Msa1120190:intron 5.0%
!!! AAAATTTAATTTATTCATTT+TGG + chr7_3:69418592-69418611 Msa1120190:intron 5.0%
!!! AAATTTAATTTATTCATTTT+GGG + chr7_3:69418593-69418612 Msa1120190:intron 5.0%
GGATCAAACCTGAATCCAGC+AGG - chr7_3:69418196-69418215 None:intergenic 50.0%
TAGACATGCAATTGGAGGGG+AGG + chr7_3:69418488-69418507 Msa1120190:intron 50.0%
AATATGGACGCAAGACGAGC+TGG + chr7_3:69418710-69418729 Msa1120190:CDS 50.0%
GACGAGCTGGAAATGGTGAA+TGG + chr7_3:69418723-69418742 Msa1120190:CDS 50.0%
! TGTCAGCTGTTGGTGCCTAT+CGG + chr7_3:69419047-69419066 Msa1120190:intron 50.0%
GAAGAAGGCCACCACACTAA+TGG + chr7_3:69419100-69419119 Msa1120190:CDS 50.0%
TGCATCCTCCATTAGTGTGG+TGG - chr7_3:69419111-69419130 None:intergenic 50.0%
GGATCAAACCTGAATCCAGC+AGG - chr7_3:69418196-69418215 None:intergenic 50.0%
TAGACATGCAATTGGAGGGG+AGG + chr7_3:69418488-69418507 Msa1120190:intron 50.0%
AATATGGACGCAAGACGAGC+TGG + chr7_3:69418710-69418729 Msa1120190:CDS 50.0%
GACGAGCTGGAAATGGTGAA+TGG + chr7_3:69418723-69418742 Msa1120190:CDS 50.0%
! TGTCAGCTGTTGGTGCCTAT+CGG + chr7_3:69419047-69419066 Msa1120190:intron 50.0%
GAAGAAGGCCACCACACTAA+TGG + chr7_3:69419100-69419119 Msa1120190:CDS 50.0%
TGCATCCTCCATTAGTGTGG+TGG - chr7_3:69419111-69419130 None:intergenic 50.0%
GCTGAGCTTGATCTACCTGC+TGG + chr7_3:69418178-69418197 Msa1120190:CDS 55.0%
GACGCAAGACGAGCTGGAAA+TGG + chr7_3:69418716-69418735 Msa1120190:CDS 55.0%
! ATCGAGCTCTGATGACCCGA+TGG + chr7_3:69418837-69418856 Msa1120190:CDS 55.0%
GAAGGCCACCACACTAATGG+AGG + chr7_3:69419103-69419122 Msa1120190:CDS 55.0%
GCTGAGCTTGATCTACCTGC+TGG + chr7_3:69418178-69418197 Msa1120190:CDS 55.0%
GACGCAAGACGAGCTGGAAA+TGG + chr7_3:69418716-69418735 Msa1120190:CDS 55.0%
! ATCGAGCTCTGATGACCCGA+TGG + chr7_3:69418837-69418856 Msa1120190:CDS 55.0%
GAAGGCCACCACACTAATGG+AGG + chr7_3:69419103-69419122 Msa1120190:CDS 55.0%
AAACCTCCTGGCAGCCTCCA+TGG - chr7_3:69418316-69418335 None:intergenic 60.0%
! GAGCTCTGATGACCCGATGG+TGG + chr7_3:69418840-69418859 Msa1120190:CDS 60.0%
AAACCTCCTGGCAGCCTCCA+TGG - chr7_3:69418316-69418335 None:intergenic 60.0%
! GAGCTCTGATGACCCGATGG+TGG + chr7_3:69418840-69418859 Msa1120190:CDS 60.0%
ACTGAGCCATGGAGGCTGCC+AGG + chr7_3:69418307-69418326 Msa1120190:CDS 65.0%
ACTGAGCCATGGAGGCTGCC+AGG + chr7_3:69418307-69418326 Msa1120190:CDS 65.0%
GAGCCATGGAGGCTGCCAGG+AGG + chr7_3:69418310-69418329 Msa1120190:CDS 70.0%
GAGCCATGGAGGCTGCCAGG+AGG + chr7_3:69418310-69418329 Msa1120190:CDS 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 69418163 69419160 69418163 ID=Msa1120190;Name=Msa1120190
chr7_3 mRNA 69418163 69419160 69418163 ID=Msa1120190-mRNA-1;Parent=Msa1120190;Name=Msa1120190-mRNA-1;_AED=0.16;_eAED=0.27;_QI=0|0|0|1|1|1|3|0|178
chr7_3 exon 69418163 69418331 69418163 ID=Msa1120190-mRNA-1:exon:17577;Parent=Msa1120190-mRNA-1
chr7_3 exon 69418645 69418904 69418645 ID=Msa1120190-mRNA-1:exon:17578;Parent=Msa1120190-mRNA-1
chr7_3 exon 69419053 69419160 69419053 ID=Msa1120190-mRNA-1:exon:17579;Parent=Msa1120190-mRNA-1
chr7_3 CDS 69418163 69418331 69418163 ID=Msa1120190-mRNA-1:cds;Parent=Msa1120190-mRNA-1
chr7_3 CDS 69418645 69418904 69418645 ID=Msa1120190-mRNA-1:cds;Parent=Msa1120190-mRNA-1
chr7_3 CDS 69419053 69419160 69419053 ID=Msa1120190-mRNA-1:cds;Parent=Msa1120190-mRNA-1
Gene Sequence

>Msa1120190

ATGGCTGAAGCTAATGCTGAGCTTGATCTACCTGCTGGATTCAGGTTTGATCCGACCGATCGTCAACTTGTTGAGTGCTACCTGACGAAGAAAATCTGCAACCAACCTTTTCCAAATGTCATGTCTGAATTTGATATATTTCAAACTGAGCCATGGAGGCTGCCAGGAGGTAATAGGTACTCTTTGGAGCATAGGAAGTATTATTTCTTTGATCTCAGGAACAGTCGGTTCGAAAATATGGACGCAAGACGAGCTGGAAATGGTGAATGGAGAGTAGTTGAAAAGAACAAGGAGTATGCTCATTCAAACAATCAATTGATTGTGAGAAAGAGCACATTTGTTTACTGGAGTGGGCAAGGAAATCGAGCTCTGATGACCCGATGGTGGATGCATGAATTTGTCATCGGTAGAATTTTTGATCCAACTAGGCTGTTGGTGCCTATCGGATCTTTAAGATATGAAGGTGGCAAAACTGAGAAGAAGGCCACCACACTAATGGAGGATGCAAGTGTTTCTGCCCCTCCTCCCTCTCCATGA
Protein sequence

>Msa1120190

MAEANAELDLPAGFRFDPTDRQLVECYLTKKICNQPFPNVMSEFDIFQTEPWRLPGGNRYSLEHRKYYFFDLRNSRFENMDARRAGNGEWRVVEKNKEYAHSNNQLIVRKSTFVYWSGQGNRALMTRWWMHEFVIGRIFDPTRLLVPIGSLRYEGGKTEKKATTLMEDASVSAPPPSP*