AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1120940


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1120940 MtrunA17_Chr1g0197421 60.000 130 20 2 1 98 1 130 1.01e-43 138
Msa1120940 MtrunA17_Chr3g0116441 100.000 47 0 0 52 98 219 265 2.69e-28 106
Msa1120940 MtrunA17_Chr3g0116441 86.957 46 6 0 1 46 15 60 5.75e-24 94.4
Msa1120940 MtrunA17_Chr1g0199511 60.417 48 19 0 52 99 338 385 3.84e-17 75.1
Msa1120940 MtrunA17_Chr1g0199511 66.667 45 14 1 1 44 13 57 8.15e-16 71.2
Msa1120940 MtrunA17_Chr5g0410721 63.636 44 16 0 52 95 280 323 2.05e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1120940 AT3G48430.1 87.234 47 6 0 52 98 247 293 8.96e-25 97.1
Msa1120940 AT3G48430.1 64.865 37 13 0 1 37 17 53 6.37e-12 60.5
Msa1120940 AT5G04240.1 58.333 48 20 0 52 99 306 353 4.62e-14 66.6
Msa1120940 AT5G04240.1 64.444 45 15 1 1 44 13 57 5.50e-14 66.2
Msa1120940 AT5G46910.1 59.091 44 18 0 52 95 294 337 6.00e-11 57.8
Msa1120940 AT5G46910.2 59.091 44 18 0 52 95 294 337 7.55e-11 57.4

Find 34 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACCCTTTGGCCCTTGAATT+TGG 0.187974 7_3:-70813398 None:intergenic
AGCTGGAGTGGGTCGGTATT+CGG 0.239347 7_3:-70813284 None:intergenic
GAGGATGTTGTTAGAGTTCA+TGG 0.296735 7_3:+70813602 Msa1120940:CDS
TAGCTTGAATTATCTTCATA+TGG 0.324265 7_3:+70813529 Msa1120940:CDS
GTTGTTAGAGTTCATGGTTA+TGG 0.343745 7_3:+70813608 Msa1120940:CDS
GCTGGAGTGGGTCGGTATTC+GGG 0.344330 7_3:-70813283 None:intergenic
GAAAACCAAGCTTCCAAATT+CGG 0.381270 7_3:+70813343 Msa1120940:CDS
GGTCGGTATTCGGGTGCTAC+TGG 0.434393 7_3:-70813274 None:intergenic
CTTTGGCCCTTGAATTTGGA+AGG 0.452571 7_3:-70813394 None:intergenic
TTGAATTTGGAAGGTGGAAA+AGG 0.454957 7_3:-70813385 None:intergenic
ATTGGATCTTCGAATTCAGC+TGG 0.456740 7_3:-70813301 None:intergenic
AGCTTGAATTATCTTCATAT+GGG 0.462001 7_3:+70813530 Msa1120940:CDS
AGAGTCCGAATTTGGAAGCT+TGG 0.462536 7_3:-70813348 None:intergenic
ATCATGAAAATGTATTCGAT+TGG 0.478299 7_3:-70813319 None:intergenic
AAACCTCAACAAAGACCCTT+TGG 0.483798 7_3:-70813411 None:intergenic
GGTGGAGAGATTAACCCTCT+TGG 0.496887 7_3:+70813629 Msa1120940:CDS
GGTGCTTCTAAGACCTGGTA+TGG 0.509028 7_3:+70813551 Msa1120940:CDS
ATTTGGAAGGTGGAAAAGGA+GGG 0.511614 7_3:-70813381 None:intergenic
ATCTCTAGGAACACCATACC+AGG 0.515561 7_3:-70813564 None:intergenic
TTCCAAATTCAAGGGCCAAA+GGG 0.528642 7_3:+70813396 Msa1120940:CDS
GGGCCAAAGGGTCTTTGTTG+AGG 0.534359 7_3:+70813408 Msa1120940:CDS
AAAGCAACAGCAGCATCTCT+AGG 0.535968 7_3:-70813578 None:intergenic
AATTTGGAAGGTGGAAAAGG+AGG 0.540672 7_3:-70813382 None:intergenic
ATCTTCGAATTCAGCTGGAG+TGG 0.545061 7_3:-70813296 None:intergenic
GTATTCGGGTGCTACTGGCA+TGG 0.554461 7_3:-70813269 None:intergenic
GTTAGAGTTCATGGTTATGG+TGG 0.562896 7_3:+70813611 Msa1120940:CDS
TTTCCACCTTCCAAATTCAA+GGG 0.562964 7_3:+70813388 Msa1120940:CDS
CTTCCAAATTCAAGGGCCAA+AGG 0.564784 7_3:+70813395 Msa1120940:CDS
TCTTCGAATTCAGCTGGAGT+GGG 0.577536 7_3:-70813295 None:intergenic
ATATGGGTGCTTCTAAGACC+TGG 0.590963 7_3:+70813546 Msa1120940:CDS
CGAATTCAGCTGGAGTGGGT+CGG 0.600042 7_3:-70813291 None:intergenic
TCCATGAACTCTAACCAAGA+GGG 0.604035 7_3:-70813643 None:intergenic
TGGCCCTTGAATTTGGAAGG+TGG 0.617375 7_3:-70813391 None:intergenic
ATCCATGAACTCTAACCAAG+AGG 0.634497 7_3:-70813644 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATCATGAAAATGTATTCGAT+TGG - chr7_3:70813322-70813341 None:intergenic 25.0%
! TAGCTTGAATTATCTTCATA+TGG + chr7_3:70813529-70813548 Msa1120940:CDS 25.0%
! AGCTTGAATTATCTTCATAT+GGG + chr7_3:70813530-70813549 Msa1120940:CDS 25.0%
GAAAACCAAGCTTCCAAATT+CGG + chr7_3:70813343-70813362 Msa1120940:CDS 35.0%
TTGAATTTGGAAGGTGGAAA+AGG - chr7_3:70813388-70813407 None:intergenic 35.0%
! TTTTCCACCTTCCAAATTCA+AGG + chr7_3:70813387-70813406 Msa1120940:CDS 35.0%
TTTCCACCTTCCAAATTCAA+GGG + chr7_3:70813388-70813407 Msa1120940:CDS 35.0%
GTCTTTGTTGAGGTTTATGA+AGG + chr7_3:70813418-70813437 Msa1120940:intron 35.0%
TTTGTTGAGGTTTATGAAGG+AGG + chr7_3:70813421-70813440 Msa1120940:intron 35.0%
!! ATCTTGCCATGCTTTTTAGT+TGG + chr7_3:70813474-70813493 Msa1120940:intron 35.0%
AGCAAACCAACTAAAAAGCA+TGG - chr7_3:70813483-70813502 None:intergenic 35.0%
GTTGTTAGAGTTCATGGTTA+TGG + chr7_3:70813608-70813627 Msa1120940:CDS 35.0%
ATTGGATCTTCGAATTCAGC+TGG - chr7_3:70813304-70813323 None:intergenic 40.0%
! GATTTTGCAGAGTCCGAATT+TGG - chr7_3:70813359-70813378 None:intergenic 40.0%
ATTTGGAAGGTGGAAAAGGA+GGG - chr7_3:70813384-70813403 None:intergenic 40.0%
AATTTGGAAGGTGGAAAAGG+AGG - chr7_3:70813385-70813404 None:intergenic 40.0%
TTCCAAATTCAAGGGCCAAA+GGG + chr7_3:70813396-70813415 Msa1120940:CDS 40.0%
AAACCTCAACAAAGACCCTT+TGG - chr7_3:70813414-70813433 None:intergenic 40.0%
GTTTATGAAGGAGGAGATAC+CGG + chr7_3:70813430-70813449 Msa1120940:intron 40.0%
TTTATGAAGGAGGAGATACC+GGG + chr7_3:70813431-70813450 Msa1120940:intron 40.0%
AGCATGGCAAGATAAACCAT+AGG - chr7_3:70813467-70813486 None:intergenic 40.0%
!! TAGTTGGTTTGCTTGACATG+TGG + chr7_3:70813490-70813509 Msa1120940:intron 40.0%
GAGGATGTTGTTAGAGTTCA+TGG + chr7_3:70813602-70813621 Msa1120940:CDS 40.0%
GTTAGAGTTCATGGTTATGG+TGG + chr7_3:70813611-70813630 Msa1120940:CDS 40.0%
TCTTCGAATTCAGCTGGAGT+GGG - chr7_3:70813298-70813317 None:intergenic 45.0%
ATCTTCGAATTCAGCTGGAG+TGG - chr7_3:70813299-70813318 None:intergenic 45.0%
AGAGTCCGAATTTGGAAGCT+TGG - chr7_3:70813351-70813370 None:intergenic 45.0%
CTTTGGCCCTTGAATTTGGA+AGG - chr7_3:70813397-70813416 None:intergenic 45.0%
CTTCCAAATTCAAGGGCCAA+AGG + chr7_3:70813395-70813414 Msa1120940:CDS 45.0%
!! TTGGTTTGCTTGACATGTGG+AGG + chr7_3:70813493-70813512 Msa1120940:intron 45.0%
!! ATATGGGTGCTTCTAAGACC+TGG + chr7_3:70813546-70813565 Msa1120940:CDS 45.0%
! ATCTCTAGGAACACCATACC+AGG - chr7_3:70813567-70813586 None:intergenic 45.0%
AAAGCAACAGCAGCATCTCT+AGG - chr7_3:70813581-70813600 None:intergenic 45.0%
!!! AGATGCTGCTGTTGCTTTTG+AGG + chr7_3:70813583-70813602 Msa1120940:CDS 45.0%
TGGCCCTTGAATTTGGAAGG+TGG - chr7_3:70813394-70813413 None:intergenic 50.0%
GACCCTTTGGCCCTTGAATT+TGG - chr7_3:70813401-70813420 None:intergenic 50.0%
ACCATAGGCGAAGTAACTCC+CGG - chr7_3:70813452-70813471 None:intergenic 50.0%
!! GGTGCTTCTAAGACCTGGTA+TGG + chr7_3:70813551-70813570 Msa1120940:CDS 50.0%
GGTGGAGAGATTAACCCTCT+TGG + chr7_3:70813629-70813648 Msa1120940:CDS 50.0%
AGCTGGAGTGGGTCGGTATT+CGG - chr7_3:70813287-70813306 None:intergenic 55.0%
CGAATTCAGCTGGAGTGGGT+CGG - chr7_3:70813294-70813313 None:intergenic 55.0%
GGGCCAAAGGGTCTTTGTTG+AGG + chr7_3:70813408-70813427 Msa1120940:CDS 55.0%
ACCGGGAGTTACTTCGCCTA+TGG + chr7_3:70813448-70813467 Msa1120940:intron 55.0%
! GGTCGGTATTCGGGTGCTAC+TGG - chr7_3:70813277-70813296 None:intergenic 60.0%
GCTGGAGTGGGTCGGTATTC+GGG - chr7_3:70813286-70813305 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 70813271 70813655 70813271 ID=Msa1120940;Name=Msa1120940
chr7_3 mRNA 70813271 70813655 70813271 ID=Msa1120940-mRNA-1;Parent=Msa1120940;Name=Msa1120940-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0|1|1|1|2|0|99
chr7_3 exon 70813271 70813429 70813271 ID=Msa1120940-mRNA-1:exon:18029;Parent=Msa1120940-mRNA-1
chr7_3 exon 70813515 70813655 70813515 ID=Msa1120940-mRNA-1:exon:18030;Parent=Msa1120940-mRNA-1
chr7_3 CDS 70813271 70813429 70813271 ID=Msa1120940-mRNA-1:cds;Parent=Msa1120940-mRNA-1
chr7_3 CDS 70813515 70813655 70813515 ID=Msa1120940-mRNA-1:cds;Parent=Msa1120940-mRNA-1
Gene Sequence

>Msa1120940

ATGCCAGTAGCACCCGAATACCGACCCACTCCAGCTGAATTCGAAGATCCAATCGAATACATTTTCATGATCGAAAACCAAGCTTCCAAATTCGGACTCTGCAAAATCATCCCTCCTTTTCCACCTTCCAAATTCAAGGGCCAAAGGGTCTTTGTTGAGGATCATGATTTGCATAGCTTGAATTATCTTCATATGGGTGCTTCTAAGACCTGGTATGGTGTTCCTAGAGATGCTGCTGTTGCTTTTGAGGATGTTGTTAGAGTTCATGGTTATGGTGGAGAGATTAACCCTCTTGGTTAG

Protein sequence

>Msa1120940

MPVAPEYRPTPAEFEDPIEYIFMIENQASKFGLCKIIPPFPPSKFKGQRVFVEDHDLHSLNYLHMGASKTWYGVPRDAAVAFEDVVRVHGYGGEINPLG*