AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1121980


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1121980 MtrunA17_Chr7g0255841 100.000 92 0 0 1 92 1 92 7.13e-65 190
Msa1121980 MtrunA17_Chr3g0081631 72.222 90 24 1 4 92 2 91 1.26e-44 139
Msa1121980 MtrunA17_Chr6g0449111 72.059 68 19 0 7 74 5 72 6.26e-35 114
Msa1121980 MtrunA17_Chr3g0144631 68.354 79 21 1 1 75 1 79 5.32e-34 112
Msa1121980 MtrunA17_Chr1g0155181 59.302 86 29 1 6 85 1 86 6.14e-33 109
Msa1121980 MtrunA17_Chr3g0141601 57.143 91 35 2 6 92 1 91 3.14e-32 107
Msa1121980 MtrunA17_Chr5g0404101 63.014 73 27 0 4 76 11 83 1.24e-30 103
Msa1121980 MtrunA17_Chr8g0373131 56.716 67 28 1 9 75 2 67 7.81e-25 88.6
Msa1121980 MtrunA17_Chr8g0373111 56.716 67 28 1 9 75 2 67 8.21e-25 88.6
Msa1121980 MtrunA17_Chr8g0373121 56.716 67 28 1 9 75 2 67 8.21e-25 88.6
Msa1121980 MtrunA17_Chr3g0141641 45.055 91 41 2 11 92 2 92 3.32e-24 87.4
Msa1121980 MtrunA17_Chr5g0404301 60.317 63 24 1 10 72 2 63 9.33e-24 85.5
Msa1121980 MtrunA17_Chr8g0373101 56.452 62 26 1 11 72 4 64 6.78e-23 83.6
Msa1121980 MtrunA17_Chr5g0436501 54.930 71 32 0 12 82 27 97 1.30e-20 82.8
Msa1121980 MtrunA17_Chr8g0364741 50.667 75 37 0 6 80 4 78 2.62e-20 78.6
Msa1121980 MtrunA17_Chr1g0162081 50.000 78 39 0 1 78 1 78 1.38e-19 80.5
Msa1121980 MtrunA17_Chr8g0365011 54.167 72 33 0 9 80 5 76 2.84e-19 79.3
Msa1121980 MtrunA17_Chr3g0104071 50.685 73 36 0 2 74 33 105 9.65e-19 78.2
Msa1121980 MtrunA17_Chr5g0429521 47.826 69 36 0 11 79 28 96 5.02e-18 76.3
Msa1121980 MtrunA17_Chr7g0241761 52.174 69 33 0 12 80 35 103 5.19e-18 76.3
Msa1121980 MtrunA17_Chr1g0194071 57.627 59 24 1 11 68 10 68 6.75e-16 66.6
Msa1121980 MtrunA17_Chr1g0210471 47.619 63 33 0 12 74 24 86 7.82e-15 67.0
Msa1121980 MtrunA17_Chr7g0270711 55.000 60 26 1 11 69 10 69 3.19e-14 62.0
Msa1121980 MtrunA17_Chr4g0062741 45.205 73 36 2 6 74 1 73 4.56e-14 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1121980 AT2G21650.1 64.773 88 24 1 12 92 14 101 4.16e-38 123
Msa1121980 AT4G39250.1 64.368 87 25 1 12 92 14 100 1.32e-37 122
Msa1121980 AT1G75250.2 55.670 97 37 1 1 91 1 97 5.60e-36 117
Msa1121980 AT1G75250.1 69.333 75 23 0 1 75 1 75 1.47e-35 117
Msa1121980 AT1G19510.1 59.783 92 37 0 1 92 1 92 2.64e-29 100
Msa1121980 AT2G18328.1 62.295 61 23 0 15 75 14 74 1.84e-24 88.2
Msa1121980 AT5G05790.2 52.857 70 33 0 9 78 28 97 3.54e-20 82.0
Msa1121980 AT5G05790.1 52.857 70 33 0 9 78 28 97 3.54e-20 82.0
Msa1121980 AT4G36570.1 75.000 44 11 0 12 55 12 55 4.92e-20 76.3
Msa1121980 AT3G11280.2 52.239 67 32 0 12 78 33 99 1.03e-18 77.8
Msa1121980 AT3G11280.1 52.239 67 32 0 12 78 33 99 1.03e-18 77.8
Msa1121980 AT2G38090.1 52.174 69 33 0 12 80 29 97 2.27e-18 77.4
Msa1121980 AT5G58900.1 50.000 74 34 1 4 74 23 96 3.03e-17 74.3
Msa1121980 AT5G08520.1 52.381 63 30 0 12 74 12 74 5.44e-16 70.9
Msa1121980 AT1G49010.1 46.479 71 35 1 7 74 3 73 1.43e-14 67.4
Msa1121980 AT5G23650.1 46.377 69 37 0 6 74 7 75 2.01e-14 67.0
Msa1121980 AT5G04760.1 41.791 67 38 1 8 74 2 67 4.55e-13 62.4
Msa1121980 AT5G01200.1 42.424 66 37 1 10 74 28 93 2.05e-11 58.2

Find 33 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAATTAATAAGCTAGTTT+TGG 0.255773 7_3:+72175664 None:intergenic
AAACAGTTGTTGATTCTTCT+AGG 0.290374 7_3:+72174928 None:intergenic
ACCCCTCAATACTAGTAATT+AGG 0.339755 7_3:+72175088 None:intergenic
TTGCTGTTGATTGCACTTGT+TGG 0.365127 7_3:+72175154 None:intergenic
TCTAGCTCAATGTCAGCTTC+TGG 0.383535 7_3:-72175608 Msa1121980:CDS
GATATTAAACATATTGAGTC+AGG 0.394581 7_3:-72175437 Msa1121980:CDS
TAATGTTTCTTAACTTCCTC+AGG 0.405406 7_3:+72175476 None:intergenic
ACCTTGAATTAATTAAGAAA+TGG 0.426803 7_3:-72175633 Msa1121980:exon
GCCATTTCTTAATTAATTCA+AGG 0.445884 7_3:+72175632 None:intergenic
CAATGTCAGCTTCTGGCTCA+TGG 0.458214 7_3:-72175601 Msa1121980:CDS
ATCCTAATTACTAGTATTGA+GGG 0.459377 7_3:-72175090 Msa1121980:three_prime_UTR
CAGTGTCTCATGAGGAGAAA+AGG 0.467404 7_3:-72175382 Msa1121980:intron
TATCCTAATTACTAGTATTG+AGG 0.475214 7_3:-72175091 Msa1121980:three_prime_UTR
TTCTGGCTCATGGAGTGTTA+AGG 0.488975 7_3:-72175591 Msa1121980:CDS
TTGCACTTGTTGGTCAATGA+AGG 0.513934 7_3:+72175164 None:intergenic
TGAGAATTACTTATAAAGAG+TGG 0.517508 7_3:+72175689 None:intergenic
GCTACATTGTACCAACGATC+TGG 0.520197 7_3:+72175521 None:intergenic
TTTCTTAATTAATTCAAGGT+AGG 0.525340 7_3:+72175636 None:intergenic
TAATTAATTCAAGGTAGGAA+AGG 0.540977 7_3:+72175641 None:intergenic
AACAGCTAAAGCTCTTTCAA+AGG 0.549456 7_3:+72175559 None:intergenic
TACTTATAAAGAGTGGTAAG+TGG 0.568013 7_3:+72175696 None:intergenic
ACATTATGAACTTCTTGTTG+AGG 0.570098 7_3:-72175459 Msa1121980:CDS
ACAACAAGAAAAGCAAAGGA+TGG 0.575663 7_3:+72174959 None:intergenic
TTCAACAACAAGAAAAGCAA+AGG 0.583360 7_3:+72174955 None:intergenic
GAAAATTTCAGTGTCTCATG+AGG 0.590467 7_3:-72175390 Msa1121980:CDS
TACAATGTAGCTCATGCTGT+TGG 0.593737 7_3:-72175509 Msa1121980:CDS
AATGTAGCTCATGCTGTTGG+TGG 0.602491 7_3:-72175506 Msa1121980:CDS
ACAAAGACACTCCAGATCGT+TGG 0.612332 7_3:-72175532 Msa1121980:CDS
CAACTTTATTAAGAACGAGG+AGG 0.657197 7_3:+72174903 None:intergenic
ATATTAAACATATTGAGTCA+GGG 0.667795 7_3:-72175436 Msa1121980:CDS
TTACAACTTTATTAAGAACG+AGG 0.684701 7_3:+72174900 None:intergenic
TCCTAATTACTAGTATTGAG+GGG 0.688545 7_3:-72175089 Msa1121980:three_prime_UTR
TGTTGGTGGCAAAACTCCTG+AGG 0.702099 7_3:-72175492 Msa1121980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACAATTAATAAGCTAGTTT+TGG + chr7_3:72174844-72174863 None:intergenic 20.0%
!! TTTCTTAATTAATTCAAGGT+AGG + chr7_3:72174872-72174891 None:intergenic 20.0%
!! ACCTTGAATTAATTAAGAAA+TGG - chr7_3:72174872-72174891 Msa1121980:three_prime_UTR 20.0%
!! ATATTAAACATATTGAGTCA+GGG - chr7_3:72175069-72175088 Msa1121980:three_prime_UTR 20.0%
!!! ATTTTCTTGTAATTTGGAAA+TGG + chr7_3:72175101-72175120 None:intergenic 20.0%
!!! ACTGAAATTTTCTTGTAATT+TGG + chr7_3:72175107-72175126 None:intergenic 20.0%
!!! TACTCTTTACTTTCTTTTAT+AGG - chr7_3:72175304-72175323 Msa1121980:intron 20.0%
!!! GCTTTACTCTTTTTTTTTTT+TGG - chr7_3:72175515-72175534 Msa1121980:CDS 20.0%
!!! AACAATTAATAAGCTAGTTT+TGG + chr7_3:72174844-72174863 None:intergenic 20.0%
!! TTTCTTAATTAATTCAAGGT+AGG + chr7_3:72174872-72174891 None:intergenic 20.0%
!! ACCTTGAATTAATTAAGAAA+TGG - chr7_3:72174872-72174891 Msa1121980:three_prime_UTR 20.0%
!! ATATTAAACATATTGAGTCA+GGG - chr7_3:72175069-72175088 Msa1121980:three_prime_UTR 20.0%
!!! ATTTTCTTGTAATTTGGAAA+TGG + chr7_3:72175101-72175120 None:intergenic 20.0%
!!! ACTGAAATTTTCTTGTAATT+TGG + chr7_3:72175107-72175126 None:intergenic 20.0%
!!! TACTCTTTACTTTCTTTTAT+AGG - chr7_3:72175304-72175323 Msa1121980:intron 20.0%
!!! GCTTTACTCTTTTTTTTTTT+TGG - chr7_3:72175515-72175534 Msa1121980:CDS 20.0%
! TGAGAATTACTTATAAAGAG+TGG + chr7_3:72174819-72174838 None:intergenic 25.0%
! TAATTAATTCAAGGTAGGAA+AGG + chr7_3:72174867-72174886 None:intergenic 25.0%
! GCCATTTCTTAATTAATTCA+AGG + chr7_3:72174876-72174895 None:intergenic 25.0%
! GATATTAAACATATTGAGTC+AGG - chr7_3:72175068-72175087 Msa1121980:three_prime_UTR 25.0%
!! TTACTTTCTTTTATAGGATG+AGG - chr7_3:72175310-72175329 Msa1121980:intron 25.0%
! TATCCTAATTACTAGTATTG+AGG - chr7_3:72175414-72175433 Msa1121980:CDS 25.0%
!! ATCCTAATTACTAGTATTGA+GGG - chr7_3:72175415-72175434 Msa1121980:CDS 25.0%
! TTACAACTTTATTAAGAACG+AGG + chr7_3:72175608-72175627 None:intergenic 25.0%
! TGAGAATTACTTATAAAGAG+TGG + chr7_3:72174819-72174838 None:intergenic 25.0%
! TAATTAATTCAAGGTAGGAA+AGG + chr7_3:72174867-72174886 None:intergenic 25.0%
! GCCATTTCTTAATTAATTCA+AGG + chr7_3:72174876-72174895 None:intergenic 25.0%
! GATATTAAACATATTGAGTC+AGG - chr7_3:72175068-72175087 Msa1121980:three_prime_UTR 25.0%
!! TTACTTTCTTTTATAGGATG+AGG - chr7_3:72175310-72175329 Msa1121980:intron 25.0%
! TATCCTAATTACTAGTATTG+AGG - chr7_3:72175414-72175433 Msa1121980:CDS 25.0%
!! ATCCTAATTACTAGTATTGA+GGG - chr7_3:72175415-72175434 Msa1121980:CDS 25.0%
! TTACAACTTTATTAAGAACG+AGG + chr7_3:72175608-72175627 None:intergenic 25.0%
TACTTATAAAGAGTGGTAAG+TGG + chr7_3:72174812-72174831 None:intergenic 30.0%
TAATGTTTCTTAACTTCCTC+AGG + chr7_3:72175032-72175051 None:intergenic 30.0%
ACATTATGAACTTCTTGTTG+AGG - chr7_3:72175046-72175065 Msa1121980:three_prime_UTR 30.0%
TTATTTAACAAGCAACACGT+AGG + chr7_3:72175194-72175213 None:intergenic 30.0%
! TCCTAATTACTAGTATTGAG+GGG - chr7_3:72175416-72175435 Msa1121980:CDS 30.0%
TTCAACAACAAGAAAAGCAA+AGG + chr7_3:72175553-72175572 None:intergenic 30.0%
!! AAACAGTTGTTGATTCTTCT+AGG + chr7_3:72175580-72175599 None:intergenic 30.0%
TACTTATAAAGAGTGGTAAG+TGG + chr7_3:72174812-72174831 None:intergenic 30.0%
TAATGTTTCTTAACTTCCTC+AGG + chr7_3:72175032-72175051 None:intergenic 30.0%
ACATTATGAACTTCTTGTTG+AGG - chr7_3:72175046-72175065 Msa1121980:three_prime_UTR 30.0%
TTATTTAACAAGCAACACGT+AGG + chr7_3:72175194-72175213 None:intergenic 30.0%
! TCCTAATTACTAGTATTGAG+GGG - chr7_3:72175416-72175435 Msa1121980:CDS 30.0%
TTCAACAACAAGAAAAGCAA+AGG + chr7_3:72175553-72175572 None:intergenic 30.0%
!! AAACAGTTGTTGATTCTTCT+AGG + chr7_3:72175580-72175599 None:intergenic 30.0%
AACAGCTAAAGCTCTTTCAA+AGG + chr7_3:72174949-72174968 None:intergenic 35.0%
GAAAATTTCAGTGTCTCATG+AGG - chr7_3:72175115-72175134 Msa1121980:three_prime_UTR 35.0%
ACCCCTCAATACTAGTAATT+AGG + chr7_3:72175420-72175439 None:intergenic 35.0%
ACAACAAGAAAAGCAAAGGA+TGG + chr7_3:72175549-72175568 None:intergenic 35.0%
CAACTTTATTAAGAACGAGG+AGG + chr7_3:72175605-72175624 None:intergenic 35.0%
AACAGCTAAAGCTCTTTCAA+AGG + chr7_3:72174949-72174968 None:intergenic 35.0%
GAAAATTTCAGTGTCTCATG+AGG - chr7_3:72175115-72175134 Msa1121980:three_prime_UTR 35.0%
ACCCCTCAATACTAGTAATT+AGG + chr7_3:72175420-72175439 None:intergenic 35.0%
ACAACAAGAAAAGCAAAGGA+TGG + chr7_3:72175549-72175568 None:intergenic 35.0%
CAACTTTATTAAGAACGAGG+AGG + chr7_3:72175605-72175624 None:intergenic 35.0%
TACAATGTAGCTCATGCTGT+TGG - chr7_3:72174996-72175015 Msa1121980:three_prime_UTR 40.0%
! TTGCACTTGTTGGTCAATGA+AGG + chr7_3:72175344-72175363 None:intergenic 40.0%
! TTGCTGTTGATTGCACTTGT+TGG + chr7_3:72175354-72175373 None:intergenic 40.0%
TACAATGTAGCTCATGCTGT+TGG - chr7_3:72174996-72175015 Msa1121980:three_prime_UTR 40.0%
! TTGCACTTGTTGGTCAATGA+AGG + chr7_3:72175344-72175363 None:intergenic 40.0%
! TTGCTGTTGATTGCACTTGT+TGG + chr7_3:72175354-72175373 None:intergenic 40.0%
! TCTAGCTCAATGTCAGCTTC+TGG - chr7_3:72174897-72174916 Msa1121980:three_prime_UTR 45.0%
!! TTCTGGCTCATGGAGTGTTA+AGG - chr7_3:72174914-72174933 Msa1121980:three_prime_UTR 45.0%
ACAAAGACACTCCAGATCGT+TGG - chr7_3:72174973-72174992 Msa1121980:three_prime_UTR 45.0%
GCTACATTGTACCAACGATC+TGG + chr7_3:72174987-72175006 None:intergenic 45.0%
AATGTAGCTCATGCTGTTGG+TGG - chr7_3:72174999-72175018 Msa1121980:three_prime_UTR 45.0%
CAGTGTCTCATGAGGAGAAA+AGG - chr7_3:72175123-72175142 Msa1121980:three_prime_UTR 45.0%
! TCTAGCTCAATGTCAGCTTC+TGG - chr7_3:72174897-72174916 Msa1121980:three_prime_UTR 45.0%
!! TTCTGGCTCATGGAGTGTTA+AGG - chr7_3:72174914-72174933 Msa1121980:three_prime_UTR 45.0%
ACAAAGACACTCCAGATCGT+TGG - chr7_3:72174973-72174992 Msa1121980:three_prime_UTR 45.0%
GCTACATTGTACCAACGATC+TGG + chr7_3:72174987-72175006 None:intergenic 45.0%
AATGTAGCTCATGCTGTTGG+TGG - chr7_3:72174999-72175018 Msa1121980:three_prime_UTR 45.0%
CAGTGTCTCATGAGGAGAAA+AGG - chr7_3:72175123-72175142 Msa1121980:three_prime_UTR 45.0%
!! CAATGTCAGCTTCTGGCTCA+TGG - chr7_3:72174904-72174923 Msa1121980:three_prime_UTR 50.0%
!! TGTTGGTGGCAAAACTCCTG+AGG - chr7_3:72175013-72175032 Msa1121980:three_prime_UTR 50.0%
!! CAATGTCAGCTTCTGGCTCA+TGG - chr7_3:72174904-72174923 Msa1121980:three_prime_UTR 50.0%
!! TGTTGGTGGCAAAACTCCTG+AGG - chr7_3:72175013-72175032 Msa1121980:three_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 72174806 72175721 72174806 ID=Msa1121980;Name=Msa1121980
chr7_3 mRNA 72174806 72175721 72174806 ID=Msa1121980-mRNA-1;Parent=Msa1121980;Name=Msa1121980-mRNA-1;_AED=0.01;_eAED=0.01;_QI=85|1|1|1|1|1|2|371|92
chr7_3 exon 72174806 72175201 72174806 ID=Msa1121980-mRNA-1:exon:18665;Parent=Msa1121980-mRNA-1
chr7_3 exon 72175383 72175721 72175383 ID=Msa1121980-mRNA-1:exon:18664;Parent=Msa1121980-mRNA-1
chr7_3 five_prime_UTR 72175637 72175721 72175637 ID=Msa1121980-mRNA-1:five_prime_utr;Parent=Msa1121980-mRNA-1
chr7_3 CDS 72175383 72175636 72175383 ID=Msa1121980-mRNA-1:cds;Parent=Msa1121980-mRNA-1
chr7_3 CDS 72175177 72175201 72175177 ID=Msa1121980-mRNA-1:cds;Parent=Msa1121980-mRNA-1
chr7_3 three_prime_UTR 72174806 72175176 72174806 ID=Msa1121980-mRNA-1:three_prime_utr;Parent=Msa1121980-mRNA-1
Gene Sequence

>Msa1121980

ATGGCATCTAGCTCAATGTCAGCTTCTGGCTCATGGAGTGTTAAGGAGAATAAAGCCTTTGAAAGAGCTTTAGCTGTTTATGACAAAGACACTCCAGATCGTTGGTACAATGTAGCTCATGCTGTTGGTGGCAAAACTCCTGAGGAAGTTAAGAAACATTATGAACTTCTTGTTGAGGATATTAAACATATTGAGTCAGGGAAAGTTCCATTTCCAAATTACAAGAAAATTTCAGTGTCTCATGAGGAGAAAAGGATGAGGAACATGAGCCTTCATTGA

Protein sequence

>Msa1121980

MASSSMSASGSWSVKENKAFERALAVYDKDTPDRWYNVAHAVGGKTPEEVKKHYELLVEDIKHIESGKVPFPNYKKISVSHEEKRMRNMSLH*