AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1134900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1134900 MtrunA17_Chr7g0223971 82.046 518 53 2 60 537 1 518 0.0 795
Msa1134900 MtrunA17_Chr7g0223991 75.587 213 32 3 208 418 24 218 7.79e-101 303
Msa1134900 MtrunA17_Chr6g0473731 44.601 426 171 12 58 465 10 388 3.09e-94 294
Msa1134900 MtrunA17_Chr4g0067981 36.818 220 114 3 1 197 54 271 1.09e-40 156
Msa1134900 MtrunA17_Chr2g0294911 34.259 216 123 1 1 197 52 267 2.38e-40 154
Msa1134900 MtrunA17_Chr1g0161431 34.694 245 128 6 6 219 39 282 1.44e-39 152
Msa1134900 MtrunA17_Chr1g0149841 35.945 217 116 2 6 199 33 249 3.92e-38 146
Msa1134900 MtrunA17_Chr8g0376881 34.468 235 129 4 6 215 53 287 7.76e-37 143
Msa1134900 MtrunA17_Chr3g0123131 32.319 263 143 4 6 236 41 300 8.69e-37 144
Msa1134900 MtrunA17_Chr3g0137101 34.483 232 103 3 6 199 36 256 3.96e-36 142
Msa1134900 MtrunA17_Chr4g0009851 31.142 289 175 8 6 285 39 312 8.20e-35 133
Msa1134900 MtrunA17_Chr4g0009981 29.478 268 174 5 23 285 2 259 2.04e-34 130
Msa1134900 MtrunA17_Chr4g0009901 36.458 192 117 2 6 197 38 224 4.57e-34 130
Msa1134900 MtrunA17_Chr7g0275401 35.648 216 115 3 6 197 33 248 9.14e-34 129
Msa1134900 MtrunA17_Chr7g0275891 32.609 230 119 4 6 199 33 262 9.02e-33 128
Msa1134900 MtrunA17_Chr6g0471041 37.255 204 111 5 6 203 42 234 1.02e-32 129
Msa1134900 MtrunA17_Chr3g0134481 34.225 187 84 2 6 192 36 183 8.48e-32 121
Msa1134900 MtrunA17_Chr2g0304841 33.193 238 123 4 6 208 46 282 1.30e-31 129
Msa1134900 MtrunA17_Chr4g0074411 33.186 226 129 4 2 205 40 265 6.28e-31 127
Msa1134900 MtrunA17_Chr3g0134461 36.022 186 89 3 6 190 36 192 3.68e-30 117
Msa1134900 MtrunA17_Chr1g0149851 33.043 230 116 3 6 197 33 262 3.74e-30 124
Msa1134900 MtrunA17_Chr4g0074401 33.333 225 127 3 6 207 45 269 8.05e-29 120
Msa1134900 MtrunA17_Chr4g0074421 35.294 187 103 3 27 195 1 187 2.29e-26 106
Msa1134900 MtrunA17_Chr1g0149831 34.574 188 89 4 35 199 7 183 1.76e-24 102
Msa1134900 MtrunA17_Chr4g0009991 37.705 122 67 2 71 190 2 116 2.01e-20 87.0
Msa1134900 MtrunA17_Chr4g0031961 42.857 112 48 4 118 219 101 206 9.74e-19 87.4
Msa1134900 MtrunA17_Chr7g0255711 49.296 71 36 0 130 200 80 150 3.09e-18 85.5
Msa1134900 MtrunA17_Chr4g0011071 29.861 144 89 3 28 167 39 174 8.83e-18 81.3
Msa1134900 MtrunA17_Chr8g0391911 28.194 227 130 7 6 205 37 257 1.10e-16 80.1
Msa1134900 MtrunA17_Chr4g0040481 61.111 54 21 0 140 193 157 210 6.91e-15 77.0
Msa1134900 MtrunA17_Chr2g0324891 47.619 84 43 1 110 193 131 213 9.71e-15 76.3
Msa1134900 MtrunA17_Chr5g0400241 53.333 60 28 0 140 199 310 369 6.71e-14 74.3
Msa1134900 MtrunA17_Chr8g0383961 28.718 195 103 6 6 197 42 203 8.05e-14 71.6
Msa1134900 MtrunA17_Chr4g0054981 50.794 63 31 0 140 202 309 371 8.41e-14 73.9
Msa1134900 MtrunA17_Chr5g0417521 54.545 55 24 1 141 194 15 69 1.28e-11 66.2
Msa1134900 MtrunA17_Chr6g0461641 54.545 55 24 1 141 194 42 96 1.44e-11 66.2
Msa1134900 MtrunA17_Chr3g0091641 32.110 109 71 1 4 109 46 154 4.44e-11 65.5
Msa1134900 MtrunA17_Chr2g0321461 50.909 55 26 1 141 194 45 99 8.11e-11 63.9
Msa1134900 MtrunA17_Chr6g0469321 62.791 43 16 0 157 199 70 112 9.18e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1134900 AT3G16857.1 33.460 263 126 4 1 220 56 312 1.10e-41 159
Msa1134900 AT3G16857.2 33.460 263 126 4 1 220 56 312 1.51e-41 159
Msa1134900 AT3G16857.3 34.008 247 114 4 17 220 2 242 5.51e-40 154
Msa1134900 AT5G07210.2 36.398 261 115 7 6 217 40 298 5.34e-39 150
Msa1134900 AT5G07210.1 36.154 260 115 7 7 217 49 306 3.09e-38 149
Msa1134900 AT2G01760.1 33.040 227 116 4 4 197 33 256 4.91e-36 138
Msa1134900 AT2G25180.1 37.089 213 115 3 6 199 41 253 2.79e-35 140
Msa1134900 AT2G01760.2 33.040 227 116 4 4 197 61 284 5.08e-35 136
Msa1134900 AT4G31920.1 37.321 209 118 3 6 204 41 246 2.36e-34 137
Msa1134900 AT2G27070.1 34.231 260 120 7 7 217 44 301 1.65e-32 131
Msa1134900 AT1G67710.1 34.199 231 124 4 6 208 35 265 1.78e-31 128
Msa1134900 AT5G58080.1 31.329 316 166 11 6 277 42 350 3.36e-31 128
Msa1134900 AT2G01760.3 32.474 194 95 4 37 197 1 191 2.83e-28 115
Msa1134900 AT3G62670.1 33.495 206 119 5 6 193 63 268 4.01e-26 111
Msa1134900 AT5G49240.1 30.918 207 121 3 6 190 66 272 4.35e-25 105
Msa1134900 AT1G67710.2 34.010 197 102 4 40 208 4 200 6.69e-25 108
Msa1134900 AT1G49190.1 31.532 222 120 5 6 198 58 276 4.61e-23 102
Msa1134900 AT1G67710.3 34.444 180 90 4 57 208 12 191 1.04e-20 95.5
Msa1134900 AT3G10760.1 47.887 71 37 0 130 200 94 164 2.39e-18 87.0
Msa1134900 AT2G40970.1 50.725 69 34 0 132 200 96 164 4.86e-18 84.3
Msa1134900 AT5G05090.1 49.275 69 35 0 129 197 69 137 5.04e-18 84.7
Msa1134900 AT5G59570.2 38.261 115 58 3 97 200 88 200 6.30e-18 85.1
Msa1134900 AT5G59570.1 38.261 115 58 3 97 200 88 200 6.30e-18 85.1
Msa1134900 AT3G46640.2 49.296 71 34 1 132 200 133 203 8.47e-18 85.1
Msa1134900 AT3G46640.1 49.296 71 34 1 132 200 133 203 8.47e-18 85.1
Msa1134900 AT3G46640.3 49.296 71 34 1 132 200 133 203 1.38e-17 84.3
Msa1134900 AT5G44190.1 62.295 61 21 1 133 193 141 199 1.75e-16 82.0
Msa1134900 AT5G44190.2 62.295 61 21 1 133 193 141 199 1.93e-16 81.3
Msa1134900 AT2G20570.1 59.259 54 22 0 140 193 152 205 3.95e-14 75.1
Msa1134900 AT4G18020.8 36.975 119 64 3 97 215 260 367 6.14e-14 74.7
Msa1134900 AT4G18020.11 36.975 119 64 3 97 215 260 367 6.14e-14 74.7
Msa1134900 AT4G18020.4 36.975 119 64 3 97 215 260 367 6.14e-14 74.7
Msa1134900 AT4G18020.5 36.975 119 64 3 97 215 260 367 6.14e-14 74.7
Msa1134900 AT4G18020.10 36.975 119 64 3 97 215 260 367 6.14e-14 74.7
Msa1134900 AT4G18020.7 36.975 119 64 3 97 215 260 367 6.14e-14 74.7
Msa1134900 AT4G18020.3 36.975 119 64 3 97 215 260 367 8.49e-14 74.3
Msa1134900 AT4G18020.2 36.975 119 64 3 97 215 260 367 8.49e-14 74.3
Msa1134900 AT4G18020.1 36.975 119 64 3 97 215 260 367 8.49e-14 74.3
Msa1134900 AT4G18020.6 36.975 119 64 3 97 215 260 367 8.49e-14 74.3
Msa1134900 AT4G18020.9 36.975 119 64 3 97 215 260 367 8.49e-14 74.3
Msa1134900 AT2G20570.2 60.784 51 20 0 143 193 171 221 1.93e-13 72.8
Msa1134900 AT1G49190.2 27.857 140 94 2 6 145 58 190 3.41e-12 69.7
Msa1134900 AT3G04450.3 52.632 57 26 1 138 193 235 291 1.34e-11 66.2
Msa1134900 AT3G04450.1 46.479 71 31 2 138 201 235 305 2.09e-11 66.6
Msa1134900 AT3G04450.4 46.479 71 31 2 138 201 195 265 2.12e-11 66.2
Msa1134900 AT3G04450.2 46.479 71 31 2 138 201 195 265 2.12e-11 66.2
Msa1134900 AT5G29000.4 44.286 70 38 1 138 206 228 297 3.14e-11 65.1
Msa1134900 AT5G29000.1 40.506 79 46 1 138 215 185 263 3.54e-11 65.5
Msa1134900 AT5G29000.3 40.506 79 46 1 138 215 185 263 3.54e-11 65.5
Msa1134900 AT5G29000.2 40.506 79 46 1 138 215 228 306 3.64e-11 65.9
Msa1134900 AT3G04030.1 52.727 55 25 1 141 194 45 99 4.74e-11 65.1
Msa1134900 AT3G04030.3 52.727 55 25 1 141 194 45 99 4.95e-11 65.1

Find 85 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATTCTATTTCTGATATT+AGG 0.142363 7_4:+1599959 Msa1134900:CDS
CTGAAGACTTCAAAGATATT+TGG 0.172247 7_4:+1598526 Msa1134900:CDS
GTAAGAAACAATAACCATTT+TGG 0.206140 7_4:+1599760 Msa1134900:CDS
AAATTCTAACTTTGGATCTT+TGG 0.211667 7_4:+1599699 Msa1134900:CDS
GAACTATGAATAATGGATTA+AGG 0.228641 7_4:+1599821 Msa1134900:CDS
CAATTCCCTTACATACAAAA+AGG 0.229371 7_4:+1599403 Msa1134900:CDS
ACTTACTTTCCAATCCAATT+TGG 0.246719 7_4:-1598764 None:intergenic
CTGCTTGCTATTAACCAAAT+TGG 0.262572 7_4:+1598750 Msa1134900:CDS
GGATTCTATTTCTGATATTA+GGG 0.272190 7_4:+1599960 Msa1134900:CDS
AACTTGTGAGGCATAATATT+AGG 0.274422 7_4:-1599727 None:intergenic
AAATGTAGCAAGTCACTTAC+AGG 0.277878 7_4:+1598967 Msa1134900:CDS
ATGACACACTTACTTATTAT+AGG 0.282733 7_4:-1597628 None:intergenic
CAAAGCATGACGAGCATCTT+TGG 0.288698 7_4:-1597494 None:intergenic
TTTGGAATTCAGCTGAATAA+TGG 0.297513 7_4:+1599778 Msa1134900:CDS
CAACATGATGCGTTGGAAAT+TGG 0.299414 7_4:-1599378 None:intergenic
ACTTACTTATTATAGGTATC+TGG 0.300133 7_4:-1597621 None:intergenic
TATTCAGCTGAATTCCAAAA+TGG 0.301790 7_4:-1599774 None:intergenic
TTAAGAAACAAAAGCTTGTT+TGG 0.309580 7_4:+1598703 Msa1134900:CDS
GGAATTGGAACTATGAATAA+TGG 0.315352 7_4:+1599814 Msa1134900:CDS
TTGAAGTCTTCAGCACAAAA+AGG 0.324880 7_4:-1598516 None:intergenic
ATCTCAAAGACACTAGAAAA+TGG 0.331289 7_4:+1598471 Msa1134900:CDS
TTTACTCAACGTGACTTAGA+TGG 0.347030 7_4:+1600051 Msa1134900:CDS
TTCAAGATTGGGAACAAGTT+AGG 0.352069 7_4:+1599434 Msa1134900:CDS
AGATATTTGGAAATATGTTA+TGG 0.355548 7_4:+1598539 Msa1134900:CDS
GGCTGTTCCAAAGAAAATTC+TGG 0.359742 7_4:+1598913 Msa1134900:CDS
AACTATGAATAATGGATTAA+GGG 0.363152 7_4:+1599822 Msa1134900:CDS
TTTCATCTGGTGGGAATCAA+TGG 0.369590 7_4:+1597565 Msa1134900:CDS
AAGGGTTGGCTCTTCAAGAT+TGG 0.372343 7_4:+1599422 Msa1134900:CDS
ACTTGTGAGGCATAATATTA+GGG 0.374491 7_4:-1599726 None:intergenic
GGAGCAGAAATGGTTGGAAT+TGG 0.379114 7_4:+1599799 Msa1134900:CDS
TGCTATTAACCAAATTGGAT+TGG 0.390260 7_4:+1598755 Msa1134900:CDS
CGTTATTGCAGAATTTCATC+TGG 0.397811 7_4:+1597552 Msa1134900:CDS
GGCCATGGTCAAAACAGGTT+TGG 0.410656 7_4:+1599502 Msa1134900:CDS
TAGAGGAGAACAAGGATTGA+TGG 0.421210 7_4:+1599603 Msa1134900:CDS
CTACTTTCACTAATGAGGTT+GGG 0.423849 7_4:+1600109 Msa1134900:CDS
ATCACTCTTCAAGAAAATGA+AGG 0.424779 7_4:+1597517 Msa1134900:CDS
GCTGAATAATGGAGCAGAAA+TGG 0.428747 7_4:+1599789 Msa1134900:CDS
CTGCGAGATGTTGAAGAGAA+AGG 0.429447 7_4:+1599175 Msa1134900:CDS
CAGTGATGTCTGAAGACAAT+AGG 0.437039 7_4:+1598439 Msa1134900:intron
AGGGTTGGCTCTTCAAGATT+GGG 0.442206 7_4:+1599423 Msa1134900:CDS
GCGTTGGAAATTGGTTGAAA+TGG 0.443167 7_4:-1599369 None:intergenic
ATAATGGCCATGGTCAAAAC+AGG 0.444362 7_4:+1599497 Msa1134900:CDS
CTTCTTCAAGTTTGTTAACA+AGG 0.448083 7_4:+1599632 Msa1134900:CDS
GCTACTTTCACTAATGAGGT+TGG 0.473474 7_4:+1600108 Msa1134900:CDS
GGTTCAAGGAGGGTTTGCTC+TGG 0.484414 7_4:+1599939 Msa1134900:CDS
GCAGGATCAGTTAATAATGA+AGG 0.491730 7_4:+1600006 Msa1134900:CDS
TTCATCTGGTGGGAATCAAT+GGG 0.491841 7_4:+1597566 Msa1134900:CDS
AGTGTCTTTGAGATTAAACT+TGG 0.491853 7_4:-1598462 None:intergenic
GTTGAAGAGAAAGGAATGGT+TGG 0.491902 7_4:+1599184 Msa1134900:CDS
TCTGATATTAGGGGAGGGAG+TGG 0.493957 7_4:+1599970 Msa1134900:CDS
CTTGAATATCTCTCTCTCCT+TGG 0.495918 7_4:-1598677 None:intergenic
GATTGGGAACAAGTTAGGCT+TGG 0.497594 7_4:+1599439 Msa1134900:CDS
TAGTGCTACTTTCACTAATG+AGG 0.497764 7_4:+1600104 Msa1134900:CDS
TAGGCTTGGAAAATCAAAGT+TGG 0.508790 7_4:+1599453 Msa1134900:CDS
ATTTCTCTGATCACAGAACT+TGG 0.510262 7_4:-1599253 None:intergenic
GATGTTGTTGCGGAGAATAA+TGG 0.517615 7_4:+1599481 Msa1134900:CDS
ATTGCAGAATTTCATCTGGT+GGG 0.533922 7_4:+1597556 Msa1134900:CDS
TTCTTCAAGTTTGTTAACAA+GGG 0.536372 7_4:+1599633 Msa1134900:CDS
ACTTCAATATTTGCAAACAC+TGG 0.542849 7_4:+1599312 Msa1134900:CDS
AGTGGAAGAAAGTTTCTAGC+AGG 0.545083 7_4:+1599988 Msa1134900:CDS
AGCTAGTGGAAGCACCATCA+TGG 0.545478 7_4:-1599570 None:intergenic
GACTTAGATGGAAATGTTGT+GGG 0.546520 7_4:+1600063 Msa1134900:CDS
TATTGCAGAATTTCATCTGG+TGG 0.554479 7_4:+1597555 Msa1134900:CDS
AATAATGGAGCAGAAATGGT+TGG 0.555811 7_4:+1599793 Msa1134900:CDS
CTATTTCTGATATTAGGGGA+GGG 0.562145 7_4:+1599965 Msa1134900:CDS
TCTATATGATGAAAAGCTAG+TGG 0.562508 7_4:-1599584 None:intergenic
AATTTGGTTTGGTTCAAGGA+GGG 0.563542 7_4:+1599929 Msa1134900:CDS
GAAGAGAAAGGAATGGTTGG+AGG 0.566539 7_4:+1599187 Msa1134900:CDS
GTTTGCAAATATTGAAGTGT+TGG 0.578096 7_4:-1599307 None:intergenic
AATTGGCCAACATGATGCGT+TGG 0.583725 7_4:-1599385 None:intergenic
TCTATTTCTGATATTAGGGG+AGG 0.587568 7_4:+1599964 Msa1134900:CDS
AAATTTGGTTTGGTTCAAGG+AGG 0.591953 7_4:+1599928 Msa1134900:CDS
TCATCATATAGAGGAGAACA+AGG 0.594028 7_4:+1599595 Msa1134900:CDS
GACATTAGAAACCAGACTCA+TGG 0.599860 7_4:+1599670 Msa1134900:CDS
GATTCTATTTCTGATATTAG+GGG 0.603781 7_4:+1599961 Msa1134900:CDS
AGAAAGAGTAAAGAATGCCA+AGG 0.604346 7_4:+1598660 Msa1134900:CDS
CAATTTCCAACGCATCATGT+TGG 0.607673 7_4:+1599379 Msa1134900:CDS
TGACTTAGATGGAAATGTTG+TGG 0.614635 7_4:+1600062 Msa1134900:CDS
GTTGGAAGCTGATGTTGTTG+CGG 0.645784 7_4:+1599471 Msa1134900:CDS
AAAGTCTTCATACCTTGTTG+CGG 0.645913 7_4:-1600188 None:intergenic
TGACAAGAATCTCCGCAACA+AGG 0.646714 7_4:+1600176 Msa1134900:CDS
ACACTGGCATATCAAACAAG+AGG 0.654807 7_4:+1599328 Msa1134900:CDS
AGATGTTGAAGAGAAAGGAA+TGG 0.660542 7_4:+1599180 Msa1134900:CDS
ACATCAGAAGTAAACTTGTG+AGG 0.669577 7_4:-1599739 None:intergenic
GTTGCGGAGAATAATGGCCA+TGG 0.690083 7_4:+1599487 Msa1134900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATATTTTTCTTATGAA+AGG + chr7_4:1598795-1598814 Msa1134900:intron 10.0%
!!! AAATATATTTTTGCATGTTA+CGG - chr7_4:1597390-1597409 None:intergenic 15.0%
!!! TATAATATTTTTGTGTCATT+TGG + chr7_4:1597956-1597975 Msa1134900:intron 15.0%
!! TAATAAAGTTTAATATAACG+TGG + chr7_4:1598158-1598177 Msa1134900:intron 15.0%
!! TATAAACAACTTTAATAAGT+TGG + chr7_4:1598197-1598216 Msa1134900:intron 15.0%
!! TTATTCACCAATTGTATTTA+AGG - chr7_4:1597333-1597352 None:intergenic 20.0%
!!! AATATATTTTTGCATGTTAC+GGG - chr7_4:1597389-1597408 None:intergenic 20.0%
!! ACATGCAAAAATATATTTCA+TGG + chr7_4:1597392-1597411 Msa1134900:intron 20.0%
!!! AACTGGATTTCTAAAATAAA+TGG - chr7_4:1597803-1597822 None:intergenic 20.0%
!!! TATTTTTCATGAAAATCGTA+AGG - chr7_4:1597840-1597859 None:intergenic 20.0%
!!! TCATTAACTCTTTAAAAATG+TGG + chr7_4:1597915-1597934 Msa1134900:intron 20.0%
!!! TTAAAAATGTGGTCAAATTT+TGG + chr7_4:1597926-1597945 Msa1134900:intron 20.0%
!! AGATATTTGGAAATATGTTA+TGG + chr7_4:1598539-1598558 Msa1134900:CDS 20.0%
!!! TTAACAAGGGATTTTAAATT+TGG + chr7_4:1599646-1599665 Msa1134900:CDS 20.0%
!!! AACTATGAATAATGGATTAA+GGG + chr7_4:1599822-1599841 Msa1134900:CDS 20.0%
!!! TCTTTTGTGAATGAAAAATT+TGG + chr7_4:1599913-1599932 Msa1134900:CDS 20.0%
!!! CAACAATTTTGATTACTTAA+TGG + chr7_4:1600149-1600168 Msa1134900:CDS 20.0%
!!! ATGCATTTGATTTTGTATTC+AGG + chr7_4:1597287-1597306 Msa1134900:CDS 25.0%
! AAAGAATTTAATGAGGCATA+AGG - chr7_4:1597364-1597383 None:intergenic 25.0%
! TGCAAAAATATATTTCATGG+TGG + chr7_4:1597395-1597414 Msa1134900:intron 25.0%
! ACTTACTTATTATAGGTATC+TGG - chr7_4:1597624-1597643 None:intergenic 25.0%
! ATGACACACTTACTTATTAT+AGG - chr7_4:1597631-1597650 None:intergenic 25.0%
! AACAAGTAAGCATTAAAAAC+TGG - chr7_4:1597708-1597727 None:intergenic 25.0%
! TTATTATGCAAACATGCTTA+AGG + chr7_4:1597727-1597746 Msa1134900:intron 25.0%
! AGGTTAAGATAATAAAGAAC+TGG - chr7_4:1597820-1597839 None:intergenic 25.0%
!! TGTCATTTGGATAGATAATT+TGG + chr7_4:1597969-1597988 Msa1134900:intron 25.0%
!!! AGACATTATTTGTGTCATTT+TGG + chr7_4:1598069-1598088 Msa1134900:intron 25.0%
!! TTAAGAAACAAAAGCTTGTT+TGG + chr7_4:1598703-1598722 Msa1134900:CDS 25.0%
!! TTATATTTTCCCTAATTCAG+AGG + chr7_4:1598892-1598911 Msa1134900:intron 25.0%
! AATTCCCTTACATACAAAAA+GGG + chr7_4:1599404-1599423 Msa1134900:CDS 25.0%
!!! GAGTTTTTTGCTTGAAAAAA+TGG - chr7_4:1599547-1599566 None:intergenic 25.0%
!!! TTCTTCAAGTTTGTTAACAA+GGG + chr7_4:1599633-1599652 Msa1134900:CDS 25.0%
! GGTGTTAAAAATTCTAACTT+TGG + chr7_4:1599691-1599710 Msa1134900:CDS 25.0%
! AAATTCTAACTTTGGATCTT+TGG + chr7_4:1599699-1599718 Msa1134900:CDS 25.0%
!! GTAAGAAACAATAACCATTT+TGG + chr7_4:1599760-1599779 Msa1134900:CDS 25.0%
!!! GAACTATGAATAATGGATTA+AGG + chr7_4:1599821-1599840 Msa1134900:CDS 25.0%
! TGTGAATGAAAAATTTGGTT+TGG + chr7_4:1599918-1599937 Msa1134900:CDS 25.0%
!!! TGGATTCTATTTCTGATATT+AGG + chr7_4:1599959-1599978 Msa1134900:CDS 25.0%
!!! GGATTCTATTTCTGATATTA+GGG + chr7_4:1599960-1599979 Msa1134900:CDS 25.0%
!!! GATTCTATTTCTGATATTAG+GGG + chr7_4:1599961-1599980 Msa1134900:CDS 25.0%
! ATTGTATTTAAGGAGAGCAT+AGG - chr7_4:1597323-1597342 None:intergenic 30.0%
ATGCTCTCCTTAAATACAAT+TGG + chr7_4:1597323-1597342 Msa1134900:intron 30.0%
ACGGGAAAAAGAATTTAATG+AGG - chr7_4:1597371-1597390 None:intergenic 30.0%
ATCACTCTTCAAGAAAATGA+AGG + chr7_4:1597517-1597536 Msa1134900:CDS 30.0%
TTTAGTGCACCTATCAATTT+AGG - chr7_4:1597762-1597781 None:intergenic 30.0%
AGTCAAACTCAAAATTACTG+AGG - chr7_4:1597863-1597882 None:intergenic 30.0%
AAGAAGCTTAAAGAACTGTA+TGG - chr7_4:1598308-1598327 None:intergenic 30.0%
TGCATAAGAACTAAAGTAGT+GGG - chr7_4:1598384-1598403 None:intergenic 30.0%
ATGCATAAGAACTAAAGTAG+TGG - chr7_4:1598385-1598404 None:intergenic 30.0%
!! AGTGTCTTTGAGATTAAACT+TGG - chr7_4:1598465-1598484 None:intergenic 30.0%
ATCTCAAAGACACTAGAAAA+TGG + chr7_4:1598471-1598490 Msa1134900:CDS 30.0%
CTGAAGACTTCAAAGATATT+TGG + chr7_4:1598526-1598545 Msa1134900:CDS 30.0%
! CAATGCTTCTTTTTGTTCTT+AGG - chr7_4:1598636-1598655 None:intergenic 30.0%
! AGAAACATTTTGTGCAGATA+AGG - chr7_4:1598732-1598751 None:intergenic 30.0%
TGCTATTAACCAAATTGGAT+TGG + chr7_4:1598755-1598774 Msa1134900:CDS 30.0%
ACTTACTTTCCAATCCAATT+TGG - chr7_4:1598767-1598786 None:intergenic 30.0%
! ATGATCTCCAGAATTTTCTT+TGG - chr7_4:1598923-1598942 None:intergenic 30.0%
AAGACAACGACAAAAATTAG+TGG - chr7_4:1599079-1599098 None:intergenic 30.0%
! ATAAGATCAAGTTTTGCATC+AGG + chr7_4:1599226-1599245 Msa1134900:CDS 30.0%
TCAGAGAAATCGAAGAAAAA+AGG + chr7_4:1599266-1599285 Msa1134900:CDS 30.0%
GTTTGCAAATATTGAAGTGT+TGG - chr7_4:1599310-1599329 None:intergenic 30.0%
ACTTCAATATTTGCAAACAC+TGG + chr7_4:1599312-1599331 Msa1134900:CDS 30.0%
! CTTTTTGTATGTAAGGGAAT+TGG - chr7_4:1599405-1599424 None:intergenic 30.0%
CAATTCCCTTACATACAAAA+AGG + chr7_4:1599403-1599422 Msa1134900:CDS 30.0%
TCTATATGATGAAAAGCTAG+TGG - chr7_4:1599587-1599606 None:intergenic 30.0%
!!! ACTAGCTTTTCATCATATAG+AGG + chr7_4:1599586-1599605 Msa1134900:CDS 30.0%
!! CTTCTTCAAGTTTGTTAACA+AGG + chr7_4:1599632-1599651 Msa1134900:CDS 30.0%
! AATTTTTAACACCATGAGTC+TGG - chr7_4:1599684-1599703 None:intergenic 30.0%
ACTTGTGAGGCATAATATTA+GGG - chr7_4:1599729-1599748 None:intergenic 30.0%
AACTTGTGAGGCATAATATT+AGG - chr7_4:1599730-1599749 None:intergenic 30.0%
TATTCAGCTGAATTCCAAAA+TGG - chr7_4:1599777-1599796 None:intergenic 30.0%
! TTTGGAATTCAGCTGAATAA+TGG + chr7_4:1599778-1599797 Msa1134900:CDS 30.0%
GGAATTGGAACTATGAATAA+TGG + chr7_4:1599814-1599833 Msa1134900:CDS 30.0%
! ATAGTTTTTGCTTCAACGAA+AGG + chr7_4:1599863-1599882 Msa1134900:CDS 30.0%
! TAGTTTTTGCTTCAACGAAA+GGG + chr7_4:1599864-1599883 Msa1134900:CDS 30.0%
!! GAAAAATTTGGTTTGGTTCA+AGG + chr7_4:1599925-1599944 Msa1134900:CDS 30.0%
CGTTATTGCAGAATTTCATC+TGG + chr7_4:1597552-1597571 Msa1134900:CDS 35.0%
TATTGCAGAATTTCATCTGG+TGG + chr7_4:1597555-1597574 Msa1134900:CDS 35.0%
ATTGCAGAATTTCATCTGGT+GGG + chr7_4:1597556-1597575 Msa1134900:CDS 35.0%
!! TTGTTTTTCTTTGCATGCAC+AGG + chr7_4:1597677-1597696 Msa1134900:intron 35.0%
! TGTTTTTCTTTGCATGCACA+GGG + chr7_4:1597678-1597697 Msa1134900:intron 35.0%
TGCAAACATGCTTAAGGATT+TGG + chr7_4:1597733-1597752 Msa1134900:intron 35.0%
GCAAACATGCTTAAGGATTT+GGG + chr7_4:1597734-1597753 Msa1134900:intron 35.0%
ATTTGGGCTCCTAAATTGAT+AGG + chr7_4:1597750-1597769 Msa1134900:intron 35.0%
TTGAAGTCTTCAGCACAAAA+AGG - chr7_4:1598519-1598538 None:intergenic 35.0%
AGAAAGAGTAAAGAATGCCA+AGG + chr7_4:1598660-1598679 Msa1134900:CDS 35.0%
!! CTGCTTGCTATTAACCAAAT+TGG + chr7_4:1598750-1598769 Msa1134900:CDS 35.0%
AAATGTAGCAAGTCACTTAC+AGG + chr7_4:1598967-1598986 Msa1134900:CDS 35.0%
AGATGTTGAAGAGAAAGGAA+TGG + chr7_4:1599180-1599199 Msa1134900:CDS 35.0%
ATTTCTCTGATCACAGAACT+TGG - chr7_4:1599256-1599275 None:intergenic 35.0%
! CCAACCCTTTTTGTATGTAA+GGG - chr7_4:1599411-1599430 None:intergenic 35.0%
TTCAAGATTGGGAACAAGTT+AGG + chr7_4:1599434-1599453 Msa1134900:CDS 35.0%
TAGGCTTGGAAAATCAAAGT+TGG + chr7_4:1599453-1599472 Msa1134900:CDS 35.0%
GCAAAAAACTCATTCCATGA+TGG + chr7_4:1599556-1599575 Msa1134900:CDS 35.0%
TCATCATATAGAGGAGAACA+AGG + chr7_4:1599595-1599614 Msa1134900:CDS 35.0%
ACATCAGAAGTAAACTTGTG+AGG - chr7_4:1599742-1599761 None:intergenic 35.0%
! AATAATGGAGCAGAAATGGT+TGG + chr7_4:1599793-1599812 Msa1134900:CDS 35.0%
! AGTTTTTGCTTCAACGAAAG+GGG + chr7_4:1599865-1599884 Msa1134900:CDS 35.0%
!! AAATTTGGTTTGGTTCAAGG+AGG + chr7_4:1599928-1599947 Msa1134900:CDS 35.0%
!! AATTTGGTTTGGTTCAAGGA+GGG + chr7_4:1599929-1599948 Msa1134900:CDS 35.0%
!! TCTATTTCTGATATTAGGGG+AGG + chr7_4:1599964-1599983 Msa1134900:CDS 35.0%
!! CTATTTCTGATATTAGGGGA+GGG + chr7_4:1599965-1599984 Msa1134900:CDS 35.0%
GCAGGATCAGTTAATAATGA+AGG + chr7_4:1600006-1600025 Msa1134900:CDS 35.0%
TTTACTCAACGTGACTTAGA+TGG + chr7_4:1600051-1600070 Msa1134900:CDS 35.0%
TGACTTAGATGGAAATGTTG+TGG + chr7_4:1600062-1600081 Msa1134900:CDS 35.0%
GACTTAGATGGAAATGTTGT+GGG + chr7_4:1600063-1600082 Msa1134900:CDS 35.0%
!! TAGTGCTACTTTCACTAATG+AGG + chr7_4:1600104-1600123 Msa1134900:CDS 35.0%
CTACTTTCACTAATGAGGTT+GGG + chr7_4:1600109-1600128 Msa1134900:CDS 35.0%
TTTCATCTGGTGGGAATCAA+TGG + chr7_4:1597565-1597584 Msa1134900:CDS 40.0%
TTCATCTGGTGGGAATCAAT+GGG + chr7_4:1597566-1597585 Msa1134900:CDS 40.0%
CAGTGATGTCTGAAGACAAT+AGG + chr7_4:1598439-1598458 Msa1134900:intron 40.0%
CTTGAATATCTCTCTCTCCT+TGG - chr7_4:1598680-1598699 None:intergenic 40.0%
TTGGAACAGCCTCTGAATTA+GGG - chr7_4:1598904-1598923 None:intergenic 40.0%
! TTTGGAACAGCCTCTGAATT+AGG - chr7_4:1598905-1598924 None:intergenic 40.0%
GGCTGTTCCAAAGAAAATTC+TGG + chr7_4:1598913-1598932 Msa1134900:CDS 40.0%
! ACATTTTCTCTGGTCAGGTT+TGG - chr7_4:1598953-1598972 None:intergenic 40.0%
! TTGCTACATTTTCTCTGGTC+AGG - chr7_4:1598958-1598977 None:intergenic 40.0%
!!! GTGACTTGCTACATTTTCTC+TGG - chr7_4:1598963-1598982 None:intergenic 40.0%
GTTGAAGAGAAAGGAATGGT+TGG + chr7_4:1599184-1599203 Msa1134900:CDS 40.0%
ACACTGGCATATCAAACAAG+AGG + chr7_4:1599328-1599347 Msa1134900:CDS 40.0%
GCGTTGGAAATTGGTTGAAA+TGG - chr7_4:1599372-1599391 None:intergenic 40.0%
CAACATGATGCGTTGGAAAT+TGG - chr7_4:1599381-1599400 None:intergenic 40.0%
CAATTTCCAACGCATCATGT+TGG + chr7_4:1599379-1599398 Msa1134900:CDS 40.0%
CCCTTACATACAAAAAGGGT+TGG + chr7_4:1599408-1599427 Msa1134900:CDS 40.0%
! GCCAACCCTTTTTGTATGTA+AGG - chr7_4:1599412-1599431 None:intergenic 40.0%
GATGTTGTTGCGGAGAATAA+TGG + chr7_4:1599481-1599500 Msa1134900:CDS 40.0%
ATAATGGCCATGGTCAAAAC+AGG + chr7_4:1599497-1599516 Msa1134900:CDS 40.0%
!!! TTCCAAACCTGTTTTGACCA+TGG - chr7_4:1599507-1599526 None:intergenic 40.0%
TAGAGGAGAACAAGGATTGA+TGG + chr7_4:1599603-1599622 Msa1134900:CDS 40.0%
GACATTAGAAACCAGACTCA+TGG + chr7_4:1599670-1599689 Msa1134900:CDS 40.0%
! GCTGAATAATGGAGCAGAAA+TGG + chr7_4:1599789-1599808 Msa1134900:CDS 40.0%
!! AGTGGAAGAAAGTTTCTAGC+AGG + chr7_4:1599988-1600007 Msa1134900:CDS 40.0%
! GCTACTTTCACTAATGAGGT+TGG + chr7_4:1600108-1600127 Msa1134900:CDS 40.0%
CAAAGCATGACGAGCATCTT+TGG - chr7_4:1597497-1597516 None:intergenic 45.0%
CTGCGAGATGTTGAAGAGAA+AGG + chr7_4:1599175-1599194 Msa1134900:CDS 45.0%
GAAGAGAAAGGAATGGTTGG+AGG + chr7_4:1599187-1599206 Msa1134900:CDS 45.0%
AATTGGCCAACATGATGCGT+TGG - chr7_4:1599388-1599407 None:intergenic 45.0%
!! AAGGGTTGGCTCTTCAAGAT+TGG + chr7_4:1599422-1599441 Msa1134900:CDS 45.0%
!! AGGGTTGGCTCTTCAAGATT+GGG + chr7_4:1599423-1599442 Msa1134900:CDS 45.0%
GATTGGGAACAAGTTAGGCT+TGG + chr7_4:1599439-1599458 Msa1134900:CDS 45.0%
GTTGGAAGCTGATGTTGTTG+CGG + chr7_4:1599471-1599490 Msa1134900:CDS 45.0%
GGAGCAGAAATGGTTGGAAT+TGG + chr7_4:1599799-1599818 Msa1134900:CDS 45.0%
TGACAAGAATCTCCGCAACA+AGG + chr7_4:1600176-1600195 Msa1134900:CDS 45.0%
!!! TATTTTGATAAATAATTTAT+AGG + chr7_4:1598102-1598121 Msa1134900:intron 5.0%
!!! ATTTTGATAAATAATTTATA+GGG + chr7_4:1598103-1598122 Msa1134900:intron 5.0%
GTTGCGGAGAATAATGGCCA+TGG + chr7_4:1599487-1599506 Msa1134900:CDS 50.0%
GGCCATGGTCAAAACAGGTT+TGG + chr7_4:1599502-1599521 Msa1134900:CDS 50.0%
!! AGCTAGTGGAAGCACCATCA+TGG - chr7_4:1599573-1599592 None:intergenic 50.0%
TCTGATATTAGGGGAGGGAG+TGG + chr7_4:1599970-1599989 Msa1134900:CDS 50.0%
! GGTTCAAGGAGGGTTTGCTC+TGG + chr7_4:1599939-1599958 Msa1134900:CDS 55.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 1597287 1600203 1597287 ID=Msa1134900;Name=Msa1134900
chr7_4 mRNA 1597287 1600203 1597287 ID=Msa1134900-mRNA-1;Parent=Msa1134900;Name=Msa1134900-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|0.83|0.6|0.5|6|0|537
chr7_4 exon 1597287 1597308 1597287 ID=Msa1134900-mRNA-1:exon:580;Parent=Msa1134900-mRNA-1
chr7_4 exon 1597485 1597637 1597485 ID=Msa1134900-mRNA-1:exon:581;Parent=Msa1134900-mRNA-1
chr7_4 exon 1598442 1598591 1598442 ID=Msa1134900-mRNA-1:exon:582;Parent=Msa1134900-mRNA-1
chr7_4 exon 1598613 1598780 1598613 ID=Msa1134900-mRNA-1:exon:583;Parent=Msa1134900-mRNA-1
chr7_4 exon 1598912 1598988 1598912 ID=Msa1134900-mRNA-1:exon:584;Parent=Msa1134900-mRNA-1
chr7_4 exon 1599160 1600203 1599160 ID=Msa1134900-mRNA-1:exon:585;Parent=Msa1134900-mRNA-1
chr7_4 CDS 1597287 1597308 1597287 ID=Msa1134900-mRNA-1:cds;Parent=Msa1134900-mRNA-1
chr7_4 CDS 1597485 1597637 1597485 ID=Msa1134900-mRNA-1:cds;Parent=Msa1134900-mRNA-1
chr7_4 CDS 1598442 1598591 1598442 ID=Msa1134900-mRNA-1:cds;Parent=Msa1134900-mRNA-1
chr7_4 CDS 1598613 1598780 1598613 ID=Msa1134900-mRNA-1:cds;Parent=Msa1134900-mRNA-1
chr7_4 CDS 1598912 1598988 1598912 ID=Msa1134900-mRNA-1:cds;Parent=Msa1134900-mRNA-1
chr7_4 CDS 1599160 1600203 1599160 ID=Msa1134900-mRNA-1:cds;Parent=Msa1134900-mRNA-1
Gene Sequence

>Msa1134900

ATGCATTTGATTTTGTATTCAGTTGTTACTGCCAAAGATGCTCGTCATGCTTTGATCACTCTTCAAGAAAATGAAGGCTTGTTTGATCTCGTTATTGCAGAATTTCATCTGGTGGGAATCAATGGGTTAGAATTTCAAAAGCATATTAAAGATCAATTCCAGATACCTATAATAATGATGTCTGAAGACAATAGGCCAAGTTTAATCTCAAAGACACTAGAAAATGGAGCTGCACAATATATTGTGAAACCTTTTTGTGCTGAAGACTTCAAAGATATTTGGAAATATGTTATGGAAGCAAAGAAAGAGAAACTTTTCATTGAAAAAGAAGAGATATCAAGTGAACCTAAGAACAAAAAGAAGCATTGCAAGAGAAAGAGTAAAGAATGCCAAGGAGAGAGAGATATTCAAGTTGTTAAGAAACAAAAGCTTGTTTGGACACCTTATCTGCACAAAATGTTTCTGCTTGCTATTAACCAAATTGGATTGGAAAAGGCTGTTCCAAAGAAAATTCTGGAGATCATGAATATACCAAACCTGACCAGAGAAAATGTAGCAAGTCACTTACAGAAGTATCGCATATTTCTGCGAGATGTTGAAGAGAAAGGAATGGTTGGAGGCATATCTCACAGAGCTATAAGATCAAGTTTTGCATCAGGTCTACCAAGTTCTGTGATCAGAGAAATCGAAGAAAAAAGGACAAACAAACTTCATGCGCCAACACTTCAATATTTGCAAACACTGGCATATCAAACAAGAGGTGCAAACAATGTTTTTAACCATTTCAACCAATTTCCAACGCATCATGTTGGCCAATTCCCTTACATACAAAAAGGGTTGGCTCTTCAAGATTGGGAACAAGTTAGGCTTGGAAAATCAAAGTTGGAAGCTGATGTTGTTGCGGAGAATAATGGCCATGGTCAAAACAGGTTTGGAATAAGCTCTTCAAAGCTTCCATTTTTTCAAGCAAAAAACTCATTCCATGATGGTGCTTCCACTAGCTTTTCATCATATAGAGGAGAACAAGGATTGATGGTTTCTTCTTCTTCAAGTTTGTTAACAAGGGATTTTAAATTTGGTGACATTAGAAACCAGACTCATGGTGTTAAAAATTCTAACTTTGGATCTTTGGCAAGCCCTAATATTATGCCTCACAAGTTTACTTCTGATGTAAGAAACAATAACCATTTTGGAATTCAGCTGAATAATGGAGCAGAAATGGTTGGAATTGGAACTATGAATAATGGATTAAGGGATTCTAGTGCAAATAACAATAGTTTTTGCTTCAACGAAAGGGGTCAAAATGAAAATATAAATTTTAGATCTTTTGTGAATGAAAAATTTGGTTTGGTTCAAGGAGGGTTTGCTCTGGATTCTATTTCTGATATTAGGGGAGGGAGTGGAAGAAAGTTTCTAGCAGGATCAGTTAATAATGAAGGAAATGTTTCAAAGAATAGACAATTTACTCAACGTGACTTAGATGGAAATGTTGTGGGAAATGAGCAAAAGTATAATAGTGCTACTTTCACTAATGAGGTTGGGTTCAACAAAATTGAATTCAACAATTTTGATTACTTAATGGATGATGACAAGAATCTCCGCAACAAGGTATGA
Protein sequence

>Msa1134900

MHLILYSVVTAKDARHALITLQENEGLFDLVIAEFHLVGINGLEFQKHIKDQFQIPIIMMSEDNRPSLISKTLENGAAQYIVKPFCAEDFKDIWKYVMEAKKEKLFIEKEEISSEPKNKKKHCKRKSKECQGERDIQVVKKQKLVWTPYLHKMFLLAINQIGLEKAVPKKILEIMNIPNLTRENVASHLQKYRIFLRDVEEKGMVGGISHRAIRSSFASGLPSSVIREIEEKRTNKLHAPTLQYLQTLAYQTRGANNVFNHFNQFPTHHVGQFPYIQKGLALQDWEQVRLGKSKLEADVVAENNGHGQNRFGISSSKLPFFQAKNSFHDGASTSFSSYRGEQGLMVSSSSSLLTRDFKFGDIRNQTHGVKNSNFGSLASPNIMPHKFTSDVRNNNHFGIQLNNGAEMVGIGTMNNGLRDSSANNNSFCFNERGQNENINFRSFVNEKFGLVQGGFALDSISDIRGGSGRKFLAGSVNNEGNVSKNRQFTQRDLDGNVVGNEQKYNSATFTNEVGFNKIEFNNFDYLMDDDKNLRNKV*