AlfalfaGEDB | Msa1138330

M. sativa cultivar zhongmu-4 / Msa1138330

KEGG: -
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1138330	KEH21732.1	57.759	116	48	1	720	835	208	322	6.29e-26	122
Msa1138330	KEH21732.1	71.512	344	88	2	370	713	1	334	1.82e-167	499
Msa1138330	KEH21732.1	94.798	346	18	0	1	346	1	346	0.0	682

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1138330	sp\|Q9FID9\|Y5389_ARATH	41.284	327	172	7	10	328	496	810	3.97e-58	217
Msa1138330	sp\|Q9FID9\|Y5389_ARATH	38.855	332	183	6	379	707	496	810	1.39e-51	198

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1138330	A0A072TWA5	57.759	116	48	1	720	835	208	322	3.00e-26	122
Msa1138330	A0A072TWA5	71.512	344	88	2	370	713	1	334	8.69e-168	499
Msa1138330	A0A072TWA5	94.798	346	18	0	1	346	1	346	0.0	682

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1138330	MtrunA17_Chr7g0219831	92.550	349	25	1	367	714	1	349	0.0	670
Msa1138330	MtrunA17_Chr7g0219831	73.851	348	80	3	1	337	4	351	3.00e-177	514
Msa1138330	MtrunA17_Chr7g0219831	61.475	122	46	1	714	835	216	336	1.51e-38	147
Msa1138330	MtrunA17_Chr7g0219841	91.040	346	17	1	1	346	1	332	0.0	645
Msa1138330	MtrunA17_Chr7g0219841	69.186	344	82	1	370	713	1	320	8.39e-164	479
Msa1138330	MtrunA17_Chr7g0219841	54.783	115	51	1	721	835	195	308	6.17e-28	115
Msa1138330	MtrunA17_Chr7g0220711	76.036	338	78	1	1	335	1	338	3.78e-178	516
Msa1138330	MtrunA17_Chr7g0220711	70.435	345	95	2	370	714	1	338	1.49e-169	494
Msa1138330	MtrunA17_Chr7g0220711	59.829	117	44	2	720	835	211	325	6.35e-34	133
Msa1138330	MtrunA17_Chr7g0219871	61.828	372	114	7	1	347	1	369	1.80e-139	418

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1138330	AT5G38990.1	41.284	327	172	7	10	328	496	810	4.04e-59	217
Msa1138330	AT5G38990.1	38.855	332	183	6	379	707	496	810	1.41e-52	198
Msa1138330	AT3G04690.1	34.722	432	246	11	289	708	391	798	5.28e-59	217
Msa1138330	AT3G04690.1	39.939	328	176	7	8	329	486	798	2.31e-57	212
Msa1138330	AT5G39000.1	40.979	327	173	7	10	328	489	803	1.09e-58	216
Msa1138330	AT5G39000.1	39.157	332	182	6	379	707	489	803	9.63e-53	198
Msa1138330	AT5G28680.1	39.144	327	186	4	8	332	490	805	1.71e-58	215
Msa1138330	AT5G28680.1	36.364	374	206	8	340	711	462	805	6.68e-58	214
Msa1138330	AT5G28680.2	39.144	327	186	4	8	332	490	805	1.71e-58	215
Msa1138330	AT5G28680.2	36.364	374	206	8	340	711	462	805	6.68e-58	214

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Find 178 sgRNAs with CRISPR-Local

Find 582 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGCTAGAAATATGCAT+TGG	0.544976	7_4:+7739957	Msa1138330:CDS
AAAAGGCTTGAAATATGCAT+TGG	0.505376	7_4:+7744585	Msa1138330:CDS
AAACGACAAGTATACGATCT+TGG	0.467235	7_4:+7746542	Msa1138330:CDS
AAAGGTTATCTAAAACATAA+TGG	0.469888	7_4:+7744336	Msa1138330:CDS
AAAGGTTATCTCAAACATAA+TGG	0.424274	7_4:+7739708	Msa1138330:CDS
AAATACTATCTTTGATAAGA+TGG	0.443976	7_4:+7745319	Msa1138330:CDS
AAATATTGTGCGCTTTGCTC+CGG	0.508431	7_4:-7744637	None:intergenic
AAATCTGACGTTTACTCCTT+TGG	0.337259	7_4:+7741142	Msa1138330:CDS
AAATGAGTATCCAGTAGCAT+TGG	0.541336	7_4:+7744362	Msa1138330:CDS
AAATGTGATGTCTATTCCTT+TGG	0.386619	7_4:+7745260	Msa1138330:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CGAAGATGCATAAATGAATA+AGG	+	chr7_4:7737746-7737765	Msa1138330:five_prime_UTR	30.0%
TCAATGAAGATAAGCACTTA+AGG	+	chr7_4:7737847-7737866	Msa1138330:five_prime_UTR	30.0%
ACTTAAGGATTTATTGATCC+AGG	+	chr7_4:7737862-7737881	Msa1138330:five_prime_UTR	30.0%
AGACATTGAAACAGTAGTTA+AGG	-	chr7_4:7738211-7738230	None:intergenic	30.0%
CAATCTTTCAGCAAGTAAAA+AGG	-	chr7_4:7738316-7738335	None:intergenic	30.0%
AAATCATTAAGAGAAACCAC+TGG	+	chr7_4:7738750-7738769	Msa1138330:intron	30.0%
CATACAACAATTTACTGGTA+TGG	+	chr7_4:7738782-7738801	Msa1138330:intron	30.0%
TGTTAAGTGAATTATGTTGC+AGG	-	chr7_4:7738870-7738889	None:intergenic	30.0%
CATACAACTACACTGAAATT+GGG	-	chr7_4:7738971-7738990	None:intergenic	30.0%
ACATACAACTACACTGAAAT+TGG	-	chr7_4:7738972-7738991	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	7737587	7747302	7737587	ID=Msa1138330;Name=Msa1138330
chr7_4	mRNA	7737587	7747302	7737587	ID=Msa1138330-mRNA-1;Parent=Msa1138330;Name=Msa1138330-mRNA-1;_AED=0.15;_eAED=0.15;_QI=315\|0.16\|0.28\|0.85\|0.83\|0.71\|7\|0\|852
chr7_4	exon	7737587	7737883	7737587	ID=Msa1138330-mRNA-1:exon:2138;Parent=Msa1138330-mRNA-1
chr7_4	exon	7739528	7740164	7739528	ID=Msa1138330-mRNA-1:exon:2139;Parent=Msa1138330-mRNA-1
chr7_4	exon	7741098	7741515	7741098	ID=Msa1138330-mRNA-1:exon:2140;Parent=Msa1138330-mRNA-1
chr7_4	exon	7744104	7744807	7744104	ID=Msa1138330-mRNA-1:exon:2141;Parent=Msa1138330-mRNA-1
chr7_4	exon	7745201	7745599	7745201	ID=Msa1138330-mRNA-1:exon:2142;Parent=Msa1138330-mRNA-1
chr7_4	exon	7746433	7746776	7746433	ID=Msa1138330-mRNA-1:exon:2143;Parent=Msa1138330-mRNA-1
chr7_4	exon	7747228	7747302	7747228	ID=Msa1138330-mRNA-1:exon:2144;Parent=Msa1138330-mRNA-1
chr7_4	five_prime_UTR	7737587	7737883	7737587	ID=Msa1138330-mRNA-1:five_prime_utr;Parent=Msa1138330-mRNA-1
chr7_4	five_prime_UTR	7739528	7739545	7739528	ID=Msa1138330-mRNA-1:five_prime_utr;Parent=Msa1138330-mRNA-1
chr7_4	CDS	7739546	7740164	7739546	ID=Msa1138330-mRNA-1:cds;Parent=Msa1138330-mRNA-1
chr7_4	CDS	7741098	7741515	7741098	ID=Msa1138330-mRNA-1:cds;Parent=Msa1138330-mRNA-1
chr7_4	CDS	7744104	7744807	7744104	ID=Msa1138330-mRNA-1:cds;Parent=Msa1138330-mRNA-1
chr7_4	CDS	7745201	7745599	7745201	ID=Msa1138330-mRNA-1:cds;Parent=Msa1138330-mRNA-1
chr7_4	CDS	7746433	7746776	7746433	ID=Msa1138330-mRNA-1:cds;Parent=Msa1138330-mRNA-1
chr7_4	CDS	7747228	7747302	7747228	ID=Msa1138330-mRNA-1:cds;Parent=Msa1138330-mRNA-1

Gene Sequence

>Msa1138330

ATGCTTGCAAAATACTCAAAGGGTAGAAGTTCGTCCAAGAAACAATATCCAACAGTTATTGAAGAGCTATGCCGTCAATTTTCTCTGGACGATCTTAAGAAATCTACCAATAACTTTGACGAACATCGAAGAATAGGGTTAACAGAATTCAGTATAGCTTACAAAGGTTATCTCAAACATAATGGTGAAACTGATTATCCAATAGCAGTGAAACGGATGATACATATATTTAATGAATGGAAGTTCAAGAAGGAAATTGAACTCAACTGCCAGCTTCATCACCCGAATTTGACGTCTTTTATAGGATTCTGTGACCACAAACATGAGAAAATTCTTGTGTATGAGTACATGTCCAATGGCTCTCTCAAAGATCACTTACTTTCGAGGGATATGGAATCGTTGTCATGGAAGAAAAGGCTAGAAATATGCATTGGAGCTGCAAAAGGACTACACTACCTTCACACTGGAACAAAGCGCGCAATTTTTCACTGTGACATAAAGCCTCAAACCATTCTTTTAGATAAGAATATGGTACCAAAACTATCGCATCTTGGGTTTTCCTTACAAGGGAAGCTTCCTAACTCAAAGCCAAAACCAATTAAAGACAACATGCTTAATGGTTACATGGCACCAAAGTATGTCCTAACCAACACTTTCACAGATAAATCTGACGTTTACTCCTTTGGTATGGTTCTACTTCAAGTGGCATGCACAAATTACAAGAATACCATCTTTGATAAGATGATCATGTTAGATGACACATATTGGTTTTCAGAAAAGTCATTTAATCCAGCTAACTTTTTGGAGAGGTTCCCAGCTAATGAGATTATTGATCCCATTCTCATGAGATTGATTGCGCCGCAAAGTTTGGAAGTGTTCATGGATATCATGAAAAGATGCTTGAATATAGAACCAAATGAAAGACCAGCTATGGGTGAGGTCGAGGTTGAACTTGAGCATGCTCTCGCATTGCAGGAGGAAGCAGATTGTGGAAAAAGTAATGGTGATTTTTACCTTTTTCCAAGCACCAGTTCTACGATTGGTTTTTATTTACCTCTGAATGTGAACCTTGATCATTGGCTATCAAACAGAAATATTATGGGAAATATGCTTGTAAAATACTCAAACCATAAAAGTTCATCCAGGAAGCAATGTCCAACATTTATAAAAGAACTATGCCATCAATTTTCTCTTGAAGATCTTAAGAAATCAACCAATAACTTTGATGAAACTCGAAAACTTGGACAATCACTATACAGTATAGTGTACAAAGGTTATCTAAAACATAATGGTGAAAATGAGTATCCAGTAGCATTGGAAAGGATGACACTTAGATTGGGCGATTGGATGTTCAAGAAGGTAATTAAACTCCACAGCCAACTTCATCACCCGAATTTGATATCTTTAATAGGATTTTGTGACCAGAAAGATGAGAAAATTATAGTGTATGAGTACATGTTCAATGGTTCTCTCTATGATCACTTACGTTCAAGGGATCTCGAATCACTCTCATGGAAGAAAAGGCTTGAAATATGCATTGGAGTTGCAAGAGGACTACACTATCTTCACACCGGAGCAAAGCGCACAATATTTCACTGTGATATAAAGCCTCAAACCATTCTTTTAGATAACCAAATGGTACCAAAACTCGCGCAACTTGGAATTTCCTTGCAAGAGAAGCGTTCTAAATCAAAGCCAACAATGATTAAACTCGACACCGATGAACTCCACAACATTATTGGTTCGACTGGCCATGGATACATGGCGCCAGAGTGTGTCCGAACCAATGCTTTCACAAATAAATGTGATGTCTATTCCTTTGGTACGGTTCTACTTGAAGTAGCAGGAACAGATTACAAAAATACTATCTTTGATAAGATGGTCAAGTTAGGGAACACAGATTGGTTTTTAGAAAAGTCATCTAATCCAGCTAACATTTTGGAGAGGTTACCAACTGATGAGATTATTGATCCCATTCTCATGAGATTGATCGCGCCGCAATGTTTGGAAGTGTTCATGGATATCATGAAAAGATGTTTGAATATAGAACCAAATGAAAGGCCGACGATAGGTGAGGTTGAGGTTGAACTTGAGCATGCTCTGGCACTGCAGGAGGAAGCAGATTGTGGAAAAAGTAATGGTACAACTGGCCATTGTTACACGGCGCCAGAGTGTGTCCGAACAAATGCTTTCACAAATAAATGTGATGTTTACTCCTTTGGTACGGTTCTACTTGATGTAGCATGCAAAAACGACAAGTATACGATCTTGGATAAGATGAAAGTGTTCATGGACAAAATTGAGTTCTTAGAAAAAGATATAATTAATGAAGTTAGCTTTTTCGAGAAGTTCCTAGATGCTGAGATTATGGATCCCATTCTTATAAGATTGATTGCACCAATATCTTTGGCAGTGTTCATGGACATCATGAAAATACGTTTGAATATAGAACCAAATGAAAAACCAACAATGGGTGACGTTGAGGCTATTCTAGGCTATGTTGTACTCACCATAACAATTAACAATCCTATGGTTGATTGGCTAGAAGAGGAACGTTAG

Protein sequence

>Msa1138330

MLAKYSKGRSSSKKQYPTVIEELCRQFSLDDLKKSTNNFDEHRRIGLTEFSIAYKGYLKHNGETDYPIAVKRMIHIFNEWKFKKEIELNCQLHHPNLTSFIGFCDHKHEKILVYEYMSNGSLKDHLLSRDMESLSWKKRLEICIGAAKGLHYLHTGTKRAIFHCDIKPQTILLDKNMVPKLSHLGFSLQGKLPNSKPKPIKDNMLNGYMAPKYVLTNTFTDKSDVYSFGMVLLQVACTNYKNTIFDKMIMLDDTYWFSEKSFNPANFLERFPANEIIDPILMRLIAPQSLEVFMDIMKRCLNIEPNERPAMGEVEVELEHALALQEEADCGKSNGDFYLFPSTSSTIGFYLPLNVNLDHWLSNRNIMGNMLVKYSNHKSSSRKQCPTFIKELCHQFSLEDLKKSTNNFDETRKLGQSLYSIVYKGYLKHNGENEYPVALERMTLRLGDWMFKKVIKLHSQLHHPNLISLIGFCDQKDEKIIVYEYMFNGSLYDHLRSRDLESLSWKKRLEICIGVARGLHYLHTGAKRTIFHCDIKPQTILLDNQMVPKLAQLGISLQEKRSKSKPTMIKLDTDELHNIIGSTGHGYMAPECVRTNAFTNKCDVYSFGTVLLEVAGTDYKNTIFDKMVKLGNTDWFLEKSSNPANILERLPTDEIIDPILMRLIAPQCLEVFMDIMKRCLNIEPNERPTIGEVEVELEHALALQEEADCGKSNGTTGHCYTAPECVRTNAFTNKCDVYSFGTVLLDVACKNDKYTILDKMKVFMDKIEFLEKDIINEVSFFEKFLDAEIMDPILIRLIAPISLAVFMDIMKIRLNIEPNEKPTMGDVEAILGYVVLTITINNPMVDWLEEER*