AlfalfaGEDB | Msa1139160

M. sativa cultivar zhongmu-4 / Msa1139160

KEGG: K07560|K11644
GO: GO:0000003 GO:0001708 GO:0002119 GO:0002164 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0007275 GO:0007423 GO:0007548 GO:0008150 GO:0009653 GO:0009791 GO:0009887 GO:0009987 GO:0022414 GO:0030154 GO:0030539 GO:0032501 GO:0032502 GO:0035112 GO:0040011 GO:0040025 GO:0045165 GO:0046661 GO:0048513 GO:0048569 GO:0048608 GO:0048731 GO:0048806 GO:0048808 GO:0048856 GO:0048869 GO:0061458 GO:0090596 GO:0090597 GO:0090598
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1139160	XP_024626129.1	70.290	138	41	1	138	1	138	4.37e-51	174
Msa1139160	XP_024626129.1	71.765	85	24	6	90	178	262	1.45e-32	126
Msa1139160	XP_024626129.1	74.803	127	32	12	138	98	224	9.38e-56	185

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1139160	sp\|O04539\|SNL4_ARATH	37.405	131	75	4	3	130	4	130	5.86e-15	73.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1139160	A0A396GZV2	70.290	138	41	1	138	1	138	2.09e-51	174
Msa1139160	A0A396GZV2	71.765	85	24	6	90	178	262	6.93e-33	126
Msa1139160	A0A396GZV2	74.803	127	32	12	138	98	224	4.48e-56	185

TFs/TRs:

Gene ID	Type	Classification
Msa1139160	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1139160	MtrunA17_Chr7g0218891	74.803	127	32	0	12	138	98	224	8.63e-60	185
Msa1139160	MtrunA17_Chr7g0218891	70.290	138	41	0	1	138	1	138	4.02e-55	174
Msa1139160	MtrunA17_Chr7g0218891	71.765	85	24	0	6	90	178	262	1.33e-36	126
Msa1139160	MtrunA17_Chr8g0364231	43.269	104	55	2	29	130	56	157	4.55e-19	82.4
Msa1139160	MtrunA17_Chr3g0077891	39.062	128	70	3	14	135	9	134	4.74e-19	78.6
Msa1139160	MtrunA17_Chr3g0077881	35.938	128	74	3	14	135	9	134	9.70e-16	70.1
Msa1139160	MtrunA17_Chr5g0415031	41.176	102	56	2	31	130	59	158	2.54e-15	71.6
Msa1139160	MtrunA17_Chr6g0452021	36.752	117	70	2	14	130	44	156	3.74e-15	70.9
Msa1139160	MtrunA17_Chr3g0077941	36.029	136	76	4	14	140	9	142	8.31e-15	67.4
Msa1139160	MtrunA17_Chr6g0452001	37.815	119	68	4	14	130	64	178	3.76e-13	65.5
Msa1139160	MtrunA17_Chr1g0205801	41.026	78	44	1	13	90	12	87	8.26e-13	60.8
Msa1139160	MtrunA17_Chr1g0206781	31.707	123	56	3	8	130	6	100	1.10e-12	60.5
Msa1139160	MtrunA17_Chr1g0205811	43.590	78	42	2	13	90	14	89	1.28e-12	60.5
Msa1139160	MtrunA17_Chr3g0077911	35.185	108	68	2	32	138	22	128	1.38e-12	61.2
Msa1139160	MtrunA17_Chr1g0205821	33.065	124	77	4	14	135	14	133	1.38e-12	61.6
Msa1139160	MtrunA17_Chr3g0077861	35.185	108	68	2	32	138	22	128	1.66e-12	61.2
Msa1139160	MtrunA17_Chr1g0205861	41.558	77	43	2	14	90	17	91	4.23e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1139160	AT1G70060.2	37.405	131	75	4	3	130	4	130	5.96e-16	73.9
Msa1139160	AT1G70060.1	37.405	131	75	4	3	130	4	130	5.96e-16	73.9
Msa1139160	AT1G24190.3	36.923	130	76	4	3	130	4	129	7.60e-16	73.6
Msa1139160	AT1G24190.2	36.923	130	76	4	3	130	4	129	8.04e-16	73.6
Msa1139160	AT1G24190.1	36.923	130	76	4	3	130	4	129	8.12e-16	73.6
Msa1139160	AT5G15020.2	41.026	117	65	4	14	130	53	165	5.10e-14	68.2
Msa1139160	AT5G15020.1	41.026	117	65	4	14	130	53	165	5.20e-14	68.2
Msa1139160	AT1G59890.2	37.607	117	69	3	14	130	46	158	8.84e-14	67.8
Msa1139160	AT1G59890.3	37.607	117	69	3	14	130	46	158	9.18e-14	67.4
Msa1139160	AT1G59890.1	37.607	117	69	3	14	130	46	158	9.45e-14	67.4
Msa1139160	AT1G59890.4	37.607	117	69	3	14	130	46	158	1.02e-13	67.4
Msa1139160	AT3G01320.1	39.496	119	65	4	14	130	58	171	1.39e-13	67.0
Msa1139160	AT1G70030.1	35.714	126	75	5	14	138	12	132	1.43e-13	64.3
Msa1139160	AT3G01320.2	39.496	119	65	4	14	130	58	171	1.46e-13	67.0
Msa1139160	AT1G10450.3	37.000	100	61	1	31	130	100	197	3.24e-12	63.2
Msa1139160	AT1G10450.2	37.000	100	61	1	31	130	49	146	4.04e-12	62.8
Msa1139160	AT1G10450.1	37.000	100	61	1	31	130	49	146	4.04e-12	62.8
Msa1139160	AT1G70030.2	33.051	118	76	3	14	130	23	138	1.76e-11	58.2
Msa1139160	AT1G27220.1	32.727	110	62	3	14	113	15	122	9.60e-11	57.4

Find 23 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTTCATTGACAAATTCTT+TGG	0.276334	7_4:-9255827	None:intergenic
TAGGATTGATGCTGTGAATC+TGG	0.308646	7_4:+9256038	Msa1139160:intron
ATAGATTTATTCAAGATAAC+AGG	0.341070	7_4:+9256223	Msa1139160:CDS
GGAACATTGTGAAGCCAAAA+AGG	0.355327	7_4:+9255804	Msa1139160:CDS
CGACTTTCTGAAAGTCTTGA+AGG	0.360292	7_4:+9255891	Msa1139160:CDS
TGACTTTCTGAAAGTCTTGA+AGG	0.381266	7_4:+9256260	Msa1139160:CDS
AACTTCAATGACAAATTCTT+CGG	0.410704	7_4:-9256196	None:intergenic
ATCGGAGGAGGAACAATCTC+TGG	0.440798	7_4:+9256152	Msa1139160:CDS
AGCTTGATTTCATATCCATC+TGG	0.485942	7_4:-9256126	None:intergenic
GAATCTGGGTAAAATATTGA+AGG	0.487796	7_4:+9256053	Msa1139160:CDS
ATATTGAAGGAATTATTTGA+AGG	0.515550	7_4:+9256066	Msa1139160:CDS
AGAGATTGTTCCTCCTCCGA+TGG	0.550257	7_4:-9256150	None:intergenic
GACTTTCTGAAAGTCTTGAA+GGG	0.558807	7_4:+9255892	Msa1139160:CDS
TCGAAATACTACATGCCAGA+TGG	0.575885	7_4:+9256111	Msa1139160:CDS
AATCAAGCTTCCATCGGAGG+AGG	0.577026	7_4:+9256140	Msa1139160:CDS
AGGATTGATGCTGTGAATCT+GGG	0.599560	7_4:+9256039	Msa1139160:intron
GATTTATTCAAGATAACAGG+AGG	0.606846	7_4:+9256226	Msa1139160:CDS
TGAAGGATTATATGACACAA+AGG	0.612910	7_4:+9256277	Msa1139160:CDS
TGAAATCAAGCTTCCATCGG+AGG	0.622504	7_4:+9256137	Msa1139160:CDS
GGGTAACCCGAGATAATGAA+AGG	0.627344	7_4:+9255772	None:intergenic
TGAAGGGTTACAAGACACAA+AGG	0.630499	7_4:+9255908	Msa1139160:CDS
ATATGAAATCAAGCTTCCAT+CGG	0.662074	7_4:+9256134	Msa1139160:CDS
ATTTATTCAAGATAACAGGA+GGG	0.684609	7_4:+9256227	Msa1139160:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTATTATATTGTTAGA+TGG	+	chr7_4:9255959-9255978	Msa1139160:intron	10.0%
!!!	ATCTAATTTATCTATCCTTT+TGG	+	chr7_4:9256006-9256025	Msa1139160:intron	20.0%
!!	ATATTGAAGGAATTATTTGA+AGG	+	chr7_4:9256066-9256085	Msa1139160:CDS	20.0%
!!	ATAGATTTATTCAAGATAAC+AGG	+	chr7_4:9256223-9256242	Msa1139160:CDS	20.0%
!!!	TTTTTTTAATTCTCACAAGA+TGG	+	chr7_4:9256300-9256319	Msa1139160:intron	20.0%
!	AACTTCATTGACAAATTCTT+TGG	-	chr7_4:9255830-9255849	None:intergenic	25.0%
!	AACTTCAATGACAAATTCTT+CGG	-	chr7_4:9256199-9256218	None:intergenic	25.0%
!	ATTTATTCAAGATAACAGGA+GGG	+	chr7_4:9256227-9256246	Msa1139160:CDS	25.0%
!	ATCCTTTTGGTACATTTTGT+AGG	+	chr7_4:9256019-9256038	Msa1139160:intron	30.0%
	ATCCTACAAAATGTACCAAA+AGG	-	chr7_4:9256024-9256043	None:intergenic	30.0%
	GAATCTGGGTAAAATATTGA+AGG	+	chr7_4:9256053-9256072	Msa1139160:CDS	30.0%
	ATATGAAATCAAGCTTCCAT+CGG	+	chr7_4:9256134-9256153	Msa1139160:CDS	30.0%
	GATTTATTCAAGATAACAGG+AGG	+	chr7_4:9256226-9256245	Msa1139160:CDS	30.0%
	TGAAGGATTATATGACACAA+AGG	+	chr7_4:9256277-9256296	Msa1139160:CDS	30.0%
!!	GACAAATTCTTTGGCCTTTT+TGG	-	chr7_4:9255821-9255840	None:intergenic	35.0%
!	GACTTTCTGAAAGTCTTGAA+GGG	+	chr7_4:9255892-9255911	Msa1139160:CDS	35.0%
	AGCTTGATTTCATATCCATC+TGG	-	chr7_4:9256129-9256148	None:intergenic	35.0%
!	GACAAATTCTTCGGCATTTT+TGG	-	chr7_4:9256190-9256209	None:intergenic	35.0%
!	TGACTTTCTGAAAGTCTTGA+AGG	+	chr7_4:9256260-9256279	Msa1139160:CDS	35.0%
	CCTGAAGATCAATGACAAAA+TGG	-	chr7_4:9256364-9256383	None:intergenic	35.0%
!	CCATTTTGTCATTGATCTTC+AGG	+	chr7_4:9256361-9256380	Msa1139160:intron	35.0%
	GGAACATTGTGAAGCCAAAA+AGG	+	chr7_4:9255804-9255823	Msa1139160:CDS	40.0%
!	CGACTTTCTGAAAGTCTTGA+AGG	+	chr7_4:9255891-9255910	Msa1139160:CDS	40.0%
	TGAAGGGTTACAAGACACAA+AGG	+	chr7_4:9255908-9255927	Msa1139160:CDS	40.0%
	TAGGATTGATGCTGTGAATC+TGG	+	chr7_4:9256038-9256057	Msa1139160:intron	40.0%
	AGGATTGATGCTGTGAATCT+GGG	+	chr7_4:9256039-9256058	Msa1139160:intron	40.0%
	TCGAAATACTACATGCCAGA+TGG	+	chr7_4:9256111-9256130	Msa1139160:CDS	40.0%
	TTCTCACAAGATGGTGCAAA+AGG	+	chr7_4:9256309-9256328	Msa1139160:intron	40.0%
	TTGTCATTGATCTTCAGGTG+TGG	+	chr7_4:9256366-9256385	Msa1139160:intron	40.0%
	TGAAATCAAGCTTCCATCGG+AGG	+	chr7_4:9256137-9256156	Msa1139160:CDS	45.0%
	CACAAGATGGTGCAAAAGGA+TGG	+	chr7_4:9256313-9256332	Msa1139160:intron	45.0%
	AATCAAGCTTCCATCGGAGG+AGG	+	chr7_4:9256140-9256159	Msa1139160:CDS	50.0%
	AGAGATTGTTCCTCCTCCGA+TGG	-	chr7_4:9256153-9256172	None:intergenic	50.0%
	ATCGGAGGAGGAACAATCTC+TGG	+	chr7_4:9256152-9256171	Msa1139160:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	9255787	9256404	9255787	ID=Msa1139160;Name=Msa1139160
chr7_4	mRNA	9255787	9256404	9255787	ID=Msa1139160-mRNA-1;Parent=Msa1139160;Name=Msa1139160-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|0\|0\|0.66\|1\|1\|3\|0\|140
chr7_4	exon	9255787	9255929	9255787	ID=Msa1139160-mRNA-1:exon:2408;Parent=Msa1139160-mRNA-1
chr7_4	exon	9256041	9256298	9256041	ID=Msa1139160-mRNA-1:exon:2409;Parent=Msa1139160-mRNA-1
chr7_4	exon	9256383	9256404	9256383	ID=Msa1139160-mRNA-1:exon:2410;Parent=Msa1139160-mRNA-1
chr7_4	CDS	9255787	9255929	9255787	ID=Msa1139160-mRNA-1:cds;Parent=Msa1139160-mRNA-1
chr7_4	CDS	9256041	9256298	9256041	ID=Msa1139160-mRNA-1:cds;Parent=Msa1139160-mRNA-1
chr7_4	CDS	9256383	9256404	9256383	ID=Msa1139160-mRNA-1:cds;Parent=Msa1139160-mRNA-1

Gene Sequence

>Msa1139160

ATGAAAGGACATTTTCTGGAACATTGTGAAGCCAAAAAGGCCAAAGAATTTGTCAATGAAGTTAAGTGTAGATTTATTCAAGATAACATGAGAGATAAGTATGACGACTTTCTGAAAGTCTTGAAGGGTTACAAGACACAAAGGATTGATGCTGTGAATCTGGGTAAAATATTGAAGGAATTATTTGAAGGTCATAGAGATCTTATATCAAGATCGAAATACTACATGCCAGATGGATATGAAATCAAGCTTCCATCGGAGGAGGAACAATCTCTGGAACATTGCGAAGCCAAAAATGCCGAAGAATTTGTCATTGAAGTTAAACATAGATTTATTCAAGATAACAGGAGGGATATGTATGATGACTTTCTGAAAGTCTTGAAGGATTATATGACACAAAGGTGTGGTGCAGCAAGATCTTAA

Protein sequence

>Msa1139160

MKGHFLEHCEAKKAKEFVNEVKCRFIQDNMRDKYDDFLKVLKGYKTQRIDAVNLGKILKELFEGHRDLISRSKYYMPDGYEIKLPSEEEQSLEHCEAKNAEEFVIEVKHRFIQDNRRDMYDDFLKVLKDYMTQRCGAARS*