Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1139740 | PNX75333.1 | 99.310 | 145 | 1 | 0 | 1 | 145 | 35 | 179 | 3.08e-96 | 303 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1139740 | sp|Q9SZL8|FRS5_ARATH | 31.579 | 152 | 94 | 4 | 2 | 145 | 119 | 268 | 7.39e-20 | 88.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1139740 | A0A2K3L9Y4 | 99.310 | 145 | 1 | 0 | 1 | 145 | 35 | 179 | 1.47e-96 | 303 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1139740 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1139740 | MtrunA17_Chr5g0448761 | 32.680 | 153 | 94 | 3 | 2 | 146 | 106 | 257 | 1.56e-23 | 95.9 |
| Msa1139740 | MtrunA17_Chr7g0258861 | 28.387 | 155 | 104 | 4 | 2 | 150 | 55 | 208 | 3.88e-15 | 70.9 |
| Msa1139740 | MtrunA17_Chr1g0198761 | 29.814 | 161 | 97 | 5 | 2 | 150 | 99 | 255 | 2.22e-14 | 69.3 |
| Msa1139740 | MtrunA17_Chr1g0202341 | 32.812 | 128 | 81 | 3 | 26 | 150 | 123 | 248 | 5.84e-13 | 65.5 |
| Msa1139740 | MtrunA17_Chr2g0324011 | 26.452 | 155 | 106 | 3 | 2 | 150 | 55 | 207 | 8.67e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1139740 | AT4G38180.1 | 31.579 | 152 | 94 | 4 | 2 | 145 | 119 | 268 | 7.51e-21 | 88.6 |
Find 26 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGTTGCTACAATGTTTC+AGG | 0.177913 | 7_4:-10414916 | Msa1139740:CDS |
| AAGCAGGGAAGAGAAGTAAA+TGG | 0.304863 | 7_4:-10415079 | Msa1139740:CDS |
| CGCAGATAGGCCACACTCTT+AGG | 0.314469 | 7_4:+10415011 | None:intergenic |
| TTCTTCTGAAATTTCTAAGA+TGG | 0.394077 | 7_4:+10414856 | None:intergenic |
| GAAGGTACACATATAGTAAA+GGG | 0.439216 | 7_4:-10415173 | Msa1139740:CDS |
| TCACTTACTGTTTCAAAAGC+AGG | 0.452498 | 7_4:-10415095 | Msa1139740:CDS |
| ATAAACAATGTTCATAATCA+TGG | 0.455589 | 7_4:-10415044 | Msa1139740:CDS |
| GTTGAGAAGTTTGATGAAGA+AGG | 0.472036 | 7_4:-10414951 | Msa1139740:CDS |
| AGCAGGGAAGAGAAGTAAAT+GGG | 0.489503 | 7_4:-10415078 | Msa1139740:CDS |
| TGAAGGTACACATATAGTAA+AGG | 0.494494 | 7_4:-10415174 | Msa1139740:CDS |
| GATGAAGAAGGAGTACCTAC+AGG | 0.503271 | 7_4:-10414939 | Msa1139740:CDS |
| GTAAAGGGTGAAAATGAAGA+AGG | 0.507431 | 7_4:-10415158 | Msa1139740:CDS |
| TTTCTTATGAGACCGCAGAT+AGG | 0.520274 | 7_4:+10414998 | None:intergenic |
| AAAGTTGCTACAATGTTTCA+GGG | 0.533512 | 7_4:-10414915 | Msa1139740:CDS |
| TGCTTTACAATCAGTTCTTG+AGG | 0.537334 | 7_4:+10415118 | None:intergenic |
| CACTTACTGTTTCAAAAGCA+GGG | 0.542698 | 7_4:-10415094 | Msa1139740:CDS |
| AGAAGAAGAAATCTTGATGT+GGG | 0.546130 | 7_4:-10414840 | Msa1139740:CDS |
| TAAAGGGTGAAAATGAAGAA+GGG | 0.554329 | 7_4:-10415157 | Msa1139740:CDS |
| CAGAAGAAGAAATCTTGATG+TGG | 0.556138 | 7_4:-10414841 | Msa1139740:CDS |
| CAATGTTCATAATCATGGAA+TGG | 0.563943 | 7_4:-10415039 | Msa1139740:CDS |
| ATTGTAGCAACTTTCCCTGT+AGG | 0.569258 | 7_4:+10414924 | None:intergenic |
| ATGTTTGTATGCGCTAATGA+AGG | 0.600535 | 7_4:-10415191 | Msa1139740:CDS |
| ATGAAGAAGGAGTACCTACA+GGG | 0.636491 | 7_4:-10414938 | Msa1139740:CDS |
| CTTCATCAAACTTCTCAACG+AGG | 0.637235 | 7_4:+10414955 | None:intergenic |
| CTAAGAGTGTGGCCTATCTG+CGG | 0.648365 | 7_4:-10415010 | Msa1139740:CDS |
| AATGGTTAGTCCTAAGAGTG+TGG | 0.677899 | 7_4:-10415021 | Msa1139740:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTATTGCATAAAAAAATT+GGG | + | chr7_4:10415172-10415191 | None:intergenic | 15.0% |
| !! | ATTGTATTGCATAAAAAAAT+TGG | + | chr7_4:10415173-10415192 | None:intergenic | 15.0% |
| !! | ATAAACAATGTTCATAATCA+TGG | - | chr7_4:10414893-10414912 | Msa1139740:CDS | 20.0% |
| !!! | AAGATAATGCTTTTTCTAGT+CGG | - | chr7_4:10415047-10415066 | Msa1139740:CDS | 25.0% |
| !!! | TTCTTCTGAAATTTCTAAGA+TGG | + | chr7_4:10415084-10415103 | None:intergenic | 25.0% |
| ! | TGAAGGTACACATATAGTAA+AGG | - | chr7_4:10414763-10414782 | Msa1139740:CDS | 30.0% |
| ! | GAAGGTACACATATAGTAAA+GGG | - | chr7_4:10414764-10414783 | Msa1139740:CDS | 30.0% |
| TAAAGGGTGAAAATGAAGAA+GGG | - | chr7_4:10414780-10414799 | Msa1139740:CDS | 30.0% | |
| CAATGTTCATAATCATGGAA+TGG | - | chr7_4:10414898-10414917 | Msa1139740:CDS | 30.0% | |
| AAAGTTGCTACAATGTTTCA+GGG | - | chr7_4:10415022-10415041 | Msa1139740:CDS | 30.0% | |
| !!! | AGATAATGCTTTTTCTAGTC+GGG | - | chr7_4:10415048-10415067 | Msa1139740:CDS | 30.0% |
| AGAAGAAGAAATCTTGATGT+GGG | - | chr7_4:10415097-10415116 | Msa1139740:CDS | 30.0% | |
| ATGTTTGTATGCGCTAATGA+AGG | - | chr7_4:10414746-10414765 | Msa1139740:CDS | 35.0% | |
| GTAAAGGGTGAAAATGAAGA+AGG | - | chr7_4:10414779-10414798 | Msa1139740:CDS | 35.0% | |
| TGCTTTACAATCAGTTCTTG+AGG | + | chr7_4:10414822-10414841 | None:intergenic | 35.0% | |
| TCACTTACTGTTTCAAAAGC+AGG | - | chr7_4:10414842-10414861 | Msa1139740:CDS | 35.0% | |
| CACTTACTGTTTCAAAAGCA+GGG | - | chr7_4:10414843-10414862 | Msa1139740:CDS | 35.0% | |
| GTTGAGAAGTTTGATGAAGA+AGG | - | chr7_4:10414986-10415005 | Msa1139740:CDS | 35.0% | |
| GAAAGTTGCTACAATGTTTC+AGG | - | chr7_4:10415021-10415040 | Msa1139740:CDS | 35.0% | |
| CAGAAGAAGAAATCTTGATG+TGG | - | chr7_4:10415096-10415115 | Msa1139740:CDS | 35.0% | |
| AAGCAGGGAAGAGAAGTAAA+TGG | - | chr7_4:10414858-10414877 | Msa1139740:CDS | 40.0% | |
| AGCAGGGAAGAGAAGTAAAT+GGG | - | chr7_4:10414859-10414878 | Msa1139740:CDS | 40.0% | |
| ! | AATGGTTAGTCCTAAGAGTG+TGG | - | chr7_4:10414916-10414935 | Msa1139740:CDS | 40.0% |
| TTTCTTATGAGACCGCAGAT+AGG | + | chr7_4:10414942-10414961 | None:intergenic | 40.0% | |
| CTTCATCAAACTTCTCAACG+AGG | + | chr7_4:10414985-10415004 | None:intergenic | 40.0% | |
| !! | ATGAAGAAGGAGTACCTACA+GGG | - | chr7_4:10414999-10415018 | Msa1139740:CDS | 40.0% |
| ATTGTAGCAACTTTCCCTGT+AGG | + | chr7_4:10415016-10415035 | None:intergenic | 40.0% | |
| !! | GATGAAGAAGGAGTACCTAC+AGG | - | chr7_4:10414998-10415017 | Msa1139740:CDS | 45.0% |
| !! | CTTTTTCTAGTCGGGATTGC+TGG | - | chr7_4:10415056-10415075 | Msa1139740:CDS | 45.0% |
| ! | CTAAGAGTGTGGCCTATCTG+CGG | - | chr7_4:10414927-10414946 | Msa1139740:CDS | 50.0% |
| CGCAGATAGGCCACACTCTT+AGG | + | chr7_4:10414929-10414948 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 10414746 | 10415213 | 10414746 | ID=Msa1139740;Name=Msa1139740 |
| chr7_4 | mRNA | 10414746 | 10415213 | 10414746 | ID=Msa1139740-mRNA-1;Parent=Msa1139740;Name=Msa1139740-mRNA-1;_AED=0.37;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|155 |
| chr7_4 | exon | 10414746 | 10415213 | 10414746 | ID=Msa1139740-mRNA-1:exon:2569;Parent=Msa1139740-mRNA-1 |
| chr7_4 | CDS | 10414746 | 10415213 | 10414746 | ID=Msa1139740-mRNA-1:cds;Parent=Msa1139740-mRNA-1 |
| Gene Sequence |
| Protein sequence |
