AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1140820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1140820 MtrunA17_Chr7g0222661 72.619 252 35 4 191 412 41 288 8.49e-111 327
Msa1140820 MtrunA17_Chr7g0222661 70.996 231 34 2 1 198 1 231 1.11e-104 311
Msa1140820 MtrunA17_Chr3g0081131 52.590 251 89 8 188 412 50 296 3.18e-71 226
Msa1140820 MtrunA17_Chr3g0081131 47.321 224 78 6 13 198 13 234 9.09e-54 181
Msa1140820 MtrunA17_Chr3g0081141 50.958 261 87 9 188 412 50 305 1.66e-64 209
Msa1140820 MtrunA17_Chr3g0081141 48.163 245 76 7 1 198 1 241 7.11e-56 186
Msa1140820 MtrunA17_Chr7g0222671 58.730 189 24 5 1 155 1 169 8.93e-59 189
Msa1140820 MtrunA17_Chr7g0222671 62.759 145 16 4 191 313 41 169 5.37e-49 164
Msa1140820 MtrunA17_Chr3g0081121 52.381 252 87 6 192 412 54 303 5.39e-57 189
Msa1140820 MtrunA17_Chr3g0081121 47.107 242 82 5 1 198 1 240 1.61e-44 156
Msa1140820 MtrunA17_Chr3g0079671 47.510 261 98 10 188 412 50 307 1.47e-51 175
Msa1140820 MtrunA17_Chr3g0079671 42.735 234 84 7 14 198 14 246 5.56e-35 131
Msa1140820 MtrunA17_Chr7g0224221 46.606 221 86 5 188 384 109 321 7.85e-49 169
Msa1140820 MtrunA17_Chr7g0224221 37.979 287 76 8 1 191 1 281 5.90e-42 151
Msa1140820 MtrunA17_Chr5g0396031 38.491 265 74 11 193 374 4 262 1.40e-29 118
Msa1140820 MtrunA17_Chr5g0396031 42.857 154 56 5 51 174 14 165 6.81e-24 102
Msa1140820 MtrunA17_Chr5g0396031 50.000 128 51 7 296 412 262 387 1.99e-17 83.6
Msa1140820 MtrunA17_Chr7g0224071 44.521 146 66 5 278 412 19 160 9.89e-20 85.9
Msa1140820 MtrunA17_Chr7g0224071 47.059 119 54 5 123 239 22 133 2.52e-15 73.6
Msa1140820 MtrunA17_Chr7g0224061 41.104 163 76 6 267 412 2 161 5.74e-18 81.3
Msa1140820 MtrunA17_Chr7g0224061 54.930 71 30 1 123 191 22 92 1.46e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1140820 AT5G01860.1 38.346 133 51 7 227 331 59 188 6.27e-11 62.4

Find 97 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATGCACGTTCTGCTTTA+TGG 0.134628 7_4:-13192459 None:intergenic
TAACTTGAGAAATAATATTA+TGG 0.179541 7_4:+13194322 Msa1140820:CDS
ACCCCAAATGGTTCTGGATA+TGG 0.215001 7_4:+13192628 Msa1140820:CDS
CCAATGCACGTTCTGCCTTA+TGG 0.226140 7_4:-13194058 None:intergenic
TTACACCTTATAATAATTAT+AGG 0.239889 7_4:+13194171 Msa1140820:CDS
TACACCTTATAATAATTATA+GGG 0.282591 7_4:+13194172 Msa1140820:CDS
CAATGCACGTTCTGCTTTAT+GGG 0.289491 7_4:-13192458 None:intergenic
CAATGCACGTTCTGCCTTAT+GGG 0.298620 7_4:-13194057 None:intergenic
TTCCCTTCATTGCTGTTATT+AGG 0.299034 7_4:-13192328 None:intergenic
TTCCCTTCATTGCTGTTATT+AGG 0.299034 7_4:-13193939 None:intergenic
AACCATATCCAGAACCATTT+GGG 0.315679 7_4:-13192630 None:intergenic
GTTGGTACAAATAATCAAAT+TGG 0.317948 7_4:+13194399 Msa1140820:CDS
AATATTATGGGTAGTAATAC+TGG 0.320029 7_4:+13194335 Msa1140820:CDS
AGAAGAGATGCTGAAGGATT+TGG 0.323349 7_4:-13192140 None:intergenic
ATCCCAAATGGTTCTAGATA+TGG 0.324554 7_4:+13194254 Msa1140820:CDS
GATGTTGAACCAACTTGAAT+AGG 0.324751 7_4:-13193909 None:intergenic
CCTTCCGATATTGACTCTTC+AGG 0.333646 7_4:+13194558 Msa1140820:CDS
TATAATAATTATAGGGGCCT+TGG 0.364440 7_4:+13194179 Msa1140820:CDS
AGGATTACCCCAAATGGTTC+TGG 0.374244 7_4:+13192622 Msa1140820:CDS
AACTTGAGAAATAATATTAT+GGG 0.376401 7_4:+13194323 Msa1140820:CDS
TCTACTTTACAAGGGTTAAG+AGG 0.386184 7_4:+13192424 Msa1140820:CDS
TAACCATATCTAGAACCATT+TGG 0.390792 7_4:-13194257 None:intergenic
AGGCCCCTATAATTATTATA+AGG 0.391284 7_4:-13194176 None:intergenic
TTTCCTAATAACAGCAATGA+AGG 0.394049 7_4:+13192325 Msa1140820:CDS
TTTCCTAATAACAGCAATGA+AGG 0.394049 7_4:+13193936 Msa1140820:CDS
AATGTTGAACCAACATCAAC+AGG 0.401018 7_4:-13192298 None:intergenic
TAACCATATCCAGAACCATT+TGG 0.403075 7_4:-13192631 None:intergenic
AACCATATCTAGAACCATTT+GGG 0.417831 7_4:-13194256 None:intergenic
GAACCATTTGGGGTAATCCT+AGG 0.423288 7_4:-13192619 None:intergenic
CTTCCGATATTGACTCTTCA+GGG 0.426999 7_4:+13194559 Msa1140820:CDS
AATATTGTGGGTAGTAATGC+TGG 0.427577 7_4:+13192709 Msa1140820:CDS
TATAATAGTTATAGGGGCCT+TGG 0.427649 7_4:+13192553 Msa1140820:intron
GTAATCCTAGGGTTAGTATA+AGG 0.431036 7_4:-13192607 None:intergenic
ATAAACCTAGGGTTAGTATA+AGG 0.431036 7_4:-13194233 None:intergenic
AACCCTAGGTTTATCCCAAA+TGG 0.444045 7_4:+13194242 Msa1140820:CDS
TTCCAGCAAGTCTGTCGCTT+CGG 0.452569 7_4:+13193857 Msa1140820:intron
TAGGGGCCTTGGTGTTAGAA+TGG 0.459014 7_4:+13192564 Msa1140820:intron
ATTGGTGCTATTCTAAAACT+TGG 0.462470 7_4:+13194417 Msa1140820:CDS
AATGGTTCTGGATATGGTTA+TGG 0.462683 7_4:+13192634 Msa1140820:CDS
GGGCAATATTCTACTTTACA+AGG 0.465182 7_4:+13192415 Msa1140820:CDS
TAGGGGCCTTGGTGTTAGAA+TGG 0.466450 7_4:+13194190 Msa1140820:CDS
CGATATTGACTCTTCAGGGT+TGG 0.468089 7_4:+13194563 Msa1140820:CDS
TTTGATGCCCTCCTAAGCCT+TGG 0.469569 7_4:-13194031 None:intergenic
AATGGTTCTAGATATGGTTA+TGG 0.471531 7_4:+13194260 Msa1140820:CDS
TCTACTCTCCAAGGCTTAGG+AGG 0.479090 7_4:+13194023 Msa1140820:CDS
GATTCTTCCCACACAAAAGA+AGG 0.481537 7_4:+13192181 Msa1140820:CDS
ATGTTGAACCAACTTGAATA+GGG 0.488992 7_4:-13193908 None:intergenic
AACCCTAGGATTACCCCAAA+TGG 0.491901 7_4:+13192616 Msa1140820:CDS
TTCCGACGAGTCTGTCGGTT+CGG 0.497329 7_4:+13192246 Msa1140820:CDS
CAACCCTGAAGAGTCAATAT+CGG 0.497568 7_4:-13194562 None:intergenic
CTACTTTACAAGGGTTAAGA+GGG 0.499687 7_4:+13192425 Msa1140820:CDS
ATGGGTAGTAATACTGGAGT+TGG 0.502250 7_4:+13194341 Msa1140820:CDS
TAATTTGAGAAATAATATTG+TGG 0.503589 7_4:+13192696 Msa1140820:CDS
AATTTGAGAAATAATATTGT+GGG 0.513997 7_4:+13192697 Msa1140820:CDS
GTTGTTAGCCCTATTCAAGT+TGG 0.516320 7_4:+13193900 Msa1140820:CDS
AATGCTGGAGTTGGAACTCT+AGG 0.516510 7_4:+13192724 Msa1140820:CDS
AATACTGGAGTTGGAACTCT+AGG 0.517102 7_4:+13194350 Msa1140820:CDS
AGGGCATCAAAATGCCCATA+AGG 0.520640 7_4:+13194043 Msa1140820:CDS
GTTGTTAGCCCTGTTGATGT+TGG 0.521421 7_4:+13192289 Msa1140820:CDS
TCTTCTGCTTCAAAAGATGA+AGG 0.525240 7_4:+13192157 Msa1140820:CDS
CTGGAGTTGGAACTCTAGGT+TGG 0.525714 7_4:+13194354 Msa1140820:CDS
GAACCATTTGGGATAAACCT+AGG 0.527743 7_4:-13194245 None:intergenic
AACCATTTGGGGTAATCCTA+GGG 0.529509 7_4:-13192618 None:intergenic
AGTACTTCAAGAATTGAAGT+TGG 0.539526 7_4:+13194381 Msa1140820:intron
TTTGCATGCTCATATTGCAC+GGG 0.543119 7_4:+13192394 Msa1140820:CDS
ACACCTTATAATAATTATAG+GGG 0.545626 7_4:+13194173 Msa1140820:CDS
AACCATTTGGGATAAACCTA+GGG 0.548903 7_4:-13194244 None:intergenic
GGAGTTGGAACTCTAGGTAT+CGG 0.552317 7_4:+13192730 Msa1140820:CDS
TTTACATGCTCGTATTGCAA+GGG 0.553334 7_4:+13193993 Msa1140820:CDS
TGAAGCAGAAGAGATGCTGA+AGG 0.553491 7_4:-13192146 None:intergenic
CTACTCTCCAAGGCTTAGGA+GGG 0.554995 7_4:+13194024 Msa1140820:CDS
CCATAAAGCAGAACGTGCAT+TGG 0.559919 7_4:+13192459 Msa1140820:CDS
GTGGGTAGTAATGCTGGAGT+TGG 0.560705 7_4:+13192715 Msa1140820:CDS
AAACACCTTATACTAACCCT+AGG 0.561390 7_4:+13192602 Msa1140820:CDS
AACCACCTTATACTAACCCT+AGG 0.567577 7_4:+13194228 Msa1140820:CDS
CCATAAGGCAGAACGTGCAT+TGG 0.569036 7_4:+13194058 Msa1140820:CDS
TTCCTAATAACAGCAATGAA+GGG 0.573815 7_4:+13192326 Msa1140820:CDS
TTCCTAATAACAGCAATGAA+GGG 0.573815 7_4:+13193937 Msa1140820:CDS
GGCAATATTCTACTTTACAA+GGG 0.582435 7_4:+13192416 Msa1140820:CDS
CTAACAACATCATCTTTCGA+TGG 0.585876 7_4:-13192274 None:intergenic
TCAAATTCCGACGAGTCTGT+CGG 0.589416 7_4:+13192241 Msa1140820:CDS
AAAAGAAGGGGTGAACATGA+AGG 0.593081 7_4:+13192195 Msa1140820:CDS
ATTCTTCCCACACAAAAGAA+GGG 0.597895 7_4:+13192182 Msa1140820:CDS
TTACATGCTCGTATTGCAAG+GGG 0.602444 7_4:+13193994 Msa1140820:CDS
GCTGAAGGATTTGGATGCCA+TGG 0.606269 7_4:-13192131 None:intergenic
AACCTAGGGTTAGTATAAGG+TGG 0.606964 7_4:-13194230 None:intergenic
TGTGGCTACTACTTCTACAA+CGG 0.618902 7_4:+13194491 Msa1140820:CDS
CAGGGTTGGACTTAACACTC+AGG 0.623642 7_4:+13194577 Msa1140820:CDS
GAAGAACGAAGAGAAGAACT+CGG 0.630413 7_4:+13192360 Msa1140820:CDS
GTTGGAACTCTAGGTATCGG+TGG 0.643491 7_4:+13192733 Msa1140820:CDS
TTCTTCCCACACAAAAGAAG+GGG 0.645372 7_4:+13192183 Msa1140820:CDS
CCTGAAGAGTCAATATCGGA+AGG 0.646766 7_4:-13194558 None:intergenic
ACCATATCCAGAACCATTTG+GGG 0.652003 7_4:-13192629 None:intergenic
TTGACTCCATTCTAACACCA+AGG 0.669121 7_4:-13192570 None:intergenic
TTGCATGCTCATATTGCACG+GGG 0.676799 7_4:+13192395 Msa1140820:CDS
TTAACTCCATTCTAACACCA+AGG 0.686015 7_4:-13194196 None:intergenic
ATGTTGAACCAACATCAACA+GGG 0.686328 7_4:-13192297 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTAAAAAAAAAATATTGAT+TGG - chr7_4:13193561-13193580 None:intergenic 10.0%
!! TAATTTGAGAAATAATATTG+TGG + chr7_4:13192696-13192715 Msa1140820:CDS 15.0%
!! AATTTGAGAAATAATATTGT+GGG + chr7_4:13192697-13192716 Msa1140820:CDS 15.0%
!! ATATTCTTCTATTAATATAG+AGG - chr7_4:13193421-13193440 None:intergenic 15.0%
!! GCAGATTTATAAATTAATAT+AGG + chr7_4:13193491-13193510 Msa1140820:intron 15.0%
!! TTACACCTTATAATAATTAT+AGG + chr7_4:13194171-13194190 Msa1140820:CDS 15.0%
!! TACACCTTATAATAATTATA+GGG + chr7_4:13194172-13194191 Msa1140820:CDS 15.0%
!! TAACTTGAGAAATAATATTA+TGG + chr7_4:13194322-13194341 Msa1140820:CDS 15.0%
!! AACTTGAGAAATAATATTAT+GGG + chr7_4:13194323-13194342 Msa1140820:CDS 15.0%
!! TTACACCTTATAATAGTTAT+AGG + chr7_4:13192545-13192564 Msa1140820:intron 20.0%
!! TACACCTTATAATAGTTATA+GGG + chr7_4:13192546-13192565 Msa1140820:intron 20.0%
!! TTCTCAAATTAAAAAGTGAA+GGG - chr7_4:13192687-13192706 None:intergenic 20.0%
!! TTTCTCAAATTAAAAAGTGA+AGG - chr7_4:13192688-13192707 None:intergenic 20.0%
!! ATAAATTATTAGTGTCAATC+TGG + chr7_4:13193002-13193021 Msa1140820:intron 20.0%
!! CAAAATGAGATTAAAAAACA+GGG - chr7_4:13193039-13193058 None:intergenic 20.0%
!! TCAAAATGAGATTAAAAAAC+AGG - chr7_4:13193040-13193059 None:intergenic 20.0%
!! TAAGAAAATAATGACAATCA+AGG - chr7_4:13193069-13193088 None:intergenic 20.0%
!!! TAGTGTTTTTTACAAAAGTT+TGG - chr7_4:13193126-13193145 None:intergenic 20.0%
!! AACTTAAAACCTTATCTTAA+AGG + chr7_4:13193252-13193271 Msa1140820:intron 20.0%
!!! ATATATGTACCTTTAAGATA+AGG - chr7_4:13193264-13193283 None:intergenic 20.0%
!! TTAATAGCATGATTGTTTAA+AGG + chr7_4:13193351-13193370 Msa1140820:intron 20.0%
!!! CTGATACTTTGAAATTTATT+TGG + chr7_4:13193521-13193540 Msa1140820:intron 20.0%
!! GTACTAGAATAATTGAAATA+AGG - chr7_4:13194146-13194165 None:intergenic 20.0%
!! ACACCTTATAATAATTATAG+GGG + chr7_4:13194173-13194192 Msa1140820:CDS 20.0%
! ACACCTTATAATAGTTATAG+GGG + chr7_4:13192547-13192566 Msa1140820:intron 25.0%
!!! TATAATGTTTGCTATGTTGT+TGG - chr7_4:13193164-13193183 None:intergenic 25.0%
! GAAACAAAATGCTATATTGT+TGG - chr7_4:13193212-13193231 None:intergenic 25.0%
! TGTTTCAAAAACTATTTCCA+AGG + chr7_4:13193226-13193245 Msa1140820:intron 25.0%
! TCTTCTATTAATATAGAGGT+GGG - chr7_4:13193417-13193436 None:intergenic 25.0%
! TTCTTCTATTAATATAGAGG+TGG - chr7_4:13193418-13193437 None:intergenic 25.0%
!!! TTGTTTATTTTGTTTGCAAG+AGG + chr7_4:13193461-13193480 Msa1140820:intron 25.0%
! TAATAGCTAAAGTAATTCAG+AGG - chr7_4:13193757-13193776 None:intergenic 25.0%
! TAGCTATTAATACAAGATGA+AGG + chr7_4:13193768-13193787 Msa1140820:intron 25.0%
! TTCTCAAGTTAAAAAGTGAA+GGG - chr7_4:13194313-13194332 None:intergenic 25.0%
! TTTCTCAAGTTAAAAAGTGA+AGG - chr7_4:13194314-13194333 None:intergenic 25.0%
! AATATTATGGGTAGTAATAC+TGG + chr7_4:13194335-13194354 Msa1140820:CDS 25.0%
! GTTGGTACAAATAATCAAAT+TGG + chr7_4:13194399-13194418 Msa1140820:CDS 25.0%
TTTCCTAATAACAGCAATGA+AGG + chr7_4:13192325-13192344 Msa1140820:CDS 30.0%
TTCCTAATAACAGCAATGAA+GGG + chr7_4:13192326-13192345 Msa1140820:CDS 30.0%
GGCAATATTCTACTTTACAA+GGG + chr7_4:13192416-13192435 Msa1140820:CDS 30.0%
TGGAAAAACAACGTAGAAAA+AGG + chr7_4:13192479-13192498 Msa1140820:CDS 30.0%
AGTACTTCAAGAATTGAAGT+TGG + chr7_4:13192760-13192779 Msa1140820:intron 30.0%
TTCCTCTTGAAGTTATGTAT+TGG + chr7_4:13192939-13192958 Msa1140820:intron 30.0%
TACCAATACATAACTTCAAG+AGG - chr7_4:13192944-13192963 None:intergenic 30.0%
!!! TAAGGTTTTAAGTTAGTCCT+TGG - chr7_4:13193246-13193265 None:intergenic 30.0%
CTTGCATGCAATTAAACAAT+TGG - chr7_4:13193587-13193606 None:intergenic 30.0%
!!! ATTTGCACCTTTTTAAGTCA+GGG - chr7_4:13193717-13193736 None:intergenic 30.0%
!!! TATTTGCACCTTTTTAAGTC+AGG - chr7_4:13193718-13193737 None:intergenic 30.0%
CTATTAATACAAGATGAAGG+TGG + chr7_4:13193771-13193790 Msa1140820:intron 30.0%
!! TTGCTGGAATTTGATTTTGA+GGG - chr7_4:13193846-13193865 None:intergenic 30.0%
ATGTTGAACCAACTTGAATA+GGG - chr7_4:13193911-13193930 None:intergenic 30.0%
TTTCCTAATAACAGCAATGA+AGG + chr7_4:13192325-13192344 Msa1140820:CDS 30.0%
TTCCTAATAACAGCAATGAA+GGG + chr7_4:13192326-13192345 Msa1140820:CDS 30.0%
TGGAAAAACAACGTAAAGAA+AGG + chr7_4:13194078-13194097 Msa1140820:CDS 30.0%
AGGCCCCTATAATTATTATA+AGG - chr7_4:13194179-13194198 None:intergenic 30.0%
TATAATAATTATAGGGGCCT+TGG + chr7_4:13194179-13194198 Msa1140820:CDS 30.0%
! ATAAACCTAGGGTTAGTATA+AGG - chr7_4:13194236-13194255 None:intergenic 30.0%
! AACCATATCTAGAACCATTT+GGG - chr7_4:13194259-13194278 None:intergenic 30.0%
! TAACCATATCTAGAACCATT+TGG - chr7_4:13194260-13194279 None:intergenic 30.0%
!! AATGGTTCTAGATATGGTTA+TGG + chr7_4:13194260-13194279 Msa1140820:CDS 30.0%
AGTACTTCAAGAATTGAAGT+TGG + chr7_4:13192760-13192779 Msa1140820:intron 30.0%
!! ATTGGTGCTATTCTAAAACT+TGG + chr7_4:13194417-13194436 Msa1140820:CDS 30.0%
TCTTCTGCTTCAAAAGATGA+AGG + chr7_4:13192157-13192176 Msa1140820:CDS 35.0%
ATTCTTCCCACACAAAAGAA+GGG + chr7_4:13192182-13192201 Msa1140820:CDS 35.0%
!! TCGTCGGAATTTGATTTTGA+GGG - chr7_4:13192235-13192254 None:intergenic 35.0%
CTAACAACATCATCTTTCGA+TGG - chr7_4:13192277-13192296 None:intergenic 35.0%
ATGTTGAACCAACATCAACA+GGG - chr7_4:13192300-13192319 None:intergenic 35.0%
AATGTTGAACCAACATCAAC+AGG - chr7_4:13192301-13192320 None:intergenic 35.0%
! TTCCCTTCATTGCTGTTATT+AGG - chr7_4:13192331-13192350 None:intergenic 35.0%
! TTTTGCATGCTCATATTGCA+CGG + chr7_4:13192393-13192412 Msa1140820:CDS 35.0%
GGGCAATATTCTACTTTACA+AGG + chr7_4:13192415-13192434 Msa1140820:CDS 35.0%
TCTACTTTACAAGGGTTAAG+AGG + chr7_4:13192424-13192443 Msa1140820:CDS 35.0%
CTACTTTACAAGGGTTAAGA+GGG + chr7_4:13192425-13192444 Msa1140820:CDS 35.0%
! CAACGTAGAAAAAGGTATGA+TGG + chr7_4:13192487-13192506 Msa1140820:intron 35.0%
AGGCCCCTATAACTATTATA+AGG - chr7_4:13192553-13192572 None:intergenic 35.0%
TATAATAGTTATAGGGGCCT+TGG + chr7_4:13192553-13192572 Msa1140820:intron 35.0%
AAACACCTTATACTAACCCT+AGG + chr7_4:13192602-13192621 Msa1140820:CDS 35.0%
! GTAATCCTAGGGTTAGTATA+AGG - chr7_4:13192610-13192629 None:intergenic 35.0%
AACCATATCCAGAACCATTT+GGG - chr7_4:13192633-13192652 None:intergenic 35.0%
TAACCATATCCAGAACCATT+TGG - chr7_4:13192634-13192653 None:intergenic 35.0%
!! AATGGTTCTGGATATGGTTA+TGG + chr7_4:13192634-13192653 Msa1140820:CDS 35.0%
AATATTGTGGGTAGTAATGC+TGG + chr7_4:13192709-13192728 Msa1140820:CDS 35.0%
!! AAGTTGGTACAAAGATGTTG+TGG + chr7_4:13192776-13192795 Msa1140820:intron 35.0%
! TTTGTTTGCAAGAGGTCTTT+TGG + chr7_4:13193469-13193488 Msa1140820:intron 35.0%
! AGCTATTATTTTCTGATGCC+AGG + chr7_4:13193655-13193674 Msa1140820:intron 35.0%
! GCTATTATTTTCTGATGCCA+GGG + chr7_4:13193656-13193675 Msa1140820:intron 35.0%
! CTATTATTTTCTGATGCCAG+GGG + chr7_4:13193657-13193676 Msa1140820:intron 35.0%
!!! TTTGCACCTTTTTAAGTCAG+GGG - chr7_4:13193716-13193735 None:intergenic 35.0%
!! CTTGCTGGAATTTGATTTTG+AGG - chr7_4:13193847-13193866 None:intergenic 35.0%
GATGTTGAACCAACTTGAAT+AGG - chr7_4:13193912-13193931 None:intergenic 35.0%
! TTCCCTTCATTGCTGTTATT+AGG - chr7_4:13192331-13192350 None:intergenic 35.0%
! TTTTACATGCTCGTATTGCA+AGG + chr7_4:13193992-13194011 Msa1140820:CDS 35.0%
TTTACATGCTCGTATTGCAA+GGG + chr7_4:13193993-13194012 Msa1140820:CDS 35.0%
! CAACGTAAAGAAAGGTATGA+TGG + chr7_4:13194086-13194105 Msa1140820:intron 35.0%
TTAACTCCATTCTAACACCA+AGG - chr7_4:13194199-13194218 None:intergenic 35.0%
! AACCATTTGGGATAAACCTA+GGG - chr7_4:13194247-13194266 None:intergenic 35.0%
!! ATCCCAAATGGTTCTAGATA+TGG + chr7_4:13194254-13194273 Msa1140820:CDS 35.0%
GACAGACAAGAAAAATGTTG+TGG + chr7_4:13194473-13194492 Msa1140820:CDS 35.0%
AGAAGAGATGCTGAAGGATT+TGG - chr7_4:13192143-13192162 None:intergenic 40.0%
GATTCTTCCCACACAAAAGA+AGG + chr7_4:13192181-13192200 Msa1140820:CDS 40.0%
TTCTTCCCACACAAAAGAAG+GGG + chr7_4:13192183-13192202 Msa1140820:CDS 40.0%
AAAAGAAGGGGTGAACATGA+AGG + chr7_4:13192195-13192214 Msa1140820:CDS 40.0%
!! CTCGTCGGAATTTGATTTTG+AGG - chr7_4:13192236-13192255 None:intergenic 40.0%
GAAGAACGAAGAGAAGAACT+CGG + chr7_4:13192360-13192379 Msa1140820:CDS 40.0%
TTTGCATGCTCATATTGCAC+GGG + chr7_4:13192394-13192413 Msa1140820:CDS 40.0%
CAATGCACGTTCTGCTTTAT+GGG - chr7_4:13192461-13192480 None:intergenic 40.0%
! CGTAGAAAAAGGTATGATGG+CGG + chr7_4:13192490-13192509 Msa1140820:intron 40.0%
TTGACTCCATTCTAACACCA+AGG - chr7_4:13192573-13192592 None:intergenic 40.0%
! AACCATTTGGGGTAATCCTA+GGG - chr7_4:13192621-13192640 None:intergenic 40.0%
ACCATATCCAGAACCATTTG+GGG - chr7_4:13192632-13192651 None:intergenic 40.0%
CCAGAAGAGTCAAAATCAGA+GGG - chr7_4:13192864-13192883 None:intergenic 40.0%
TCCAGAAGAGTCAAAATCAG+AGG - chr7_4:13192865-13192884 None:intergenic 40.0%
! TAGAGGTGGGATACACTTTT+TGG - chr7_4:13193404-13193423 None:intergenic 40.0%
TGAACACCCCTGACTTAAAA+AGG + chr7_4:13193707-13193726 Msa1140820:intron 40.0%
GTTCTACCCACACAAAAGAA+GGG + chr7_4:13193793-13193812 Msa1140820:intron 40.0%
GTTGTTAGCCCTATTCAAGT+TGG + chr7_4:13193900-13193919 Msa1140820:CDS 40.0%
TTACATGCTCGTATTGCAAG+GGG + chr7_4:13193994-13194013 Msa1140820:CDS 40.0%
! GGGAAATTTTCTACTCTCCA+AGG + chr7_4:13194014-13194033 Msa1140820:CDS 40.0%
! TTTTCTACTCTCCAAGGCTT+AGG + chr7_4:13194020-13194039 Msa1140820:CDS 40.0%
! CGTAAAGAAAGGTATGATGG+TGG + chr7_4:13194089-13194108 Msa1140820:intron 40.0%
! ATGGTGGTGCTTTAGGTTTA+GGG + chr7_4:13194105-13194124 Msa1140820:intron 40.0%
AACCACCTTATACTAACCCT+AGG + chr7_4:13194228-13194247 Msa1140820:CDS 40.0%
! AACCTAGGGTTAGTATAAGG+TGG - chr7_4:13194233-13194252 None:intergenic 40.0%
AACCCTAGGTTTATCCCAAA+TGG + chr7_4:13194242-13194261 Msa1140820:CDS 40.0%
! GAACCATTTGGGATAAACCT+AGG - chr7_4:13194248-13194267 None:intergenic 40.0%
ATGGGTAGTAATACTGGAGT+TGG + chr7_4:13194341-13194360 Msa1140820:CDS 40.0%
AATACTGGAGTTGGAACTCT+AGG + chr7_4:13194350-13194369 Msa1140820:CDS 40.0%
TGTGGCTACTACTTCTACAA+CGG + chr7_4:13194491-13194510 Msa1140820:CDS 40.0%
!! CTTCCGATATTGACTCTTCA+GGG + chr7_4:13194559-13194578 Msa1140820:CDS 40.0%
CAACCCTGAAGAGTCAATAT+CGG - chr7_4:13194565-13194584 None:intergenic 40.0%
TGAAGCAGAAGAGATGCTGA+AGG - chr7_4:13192149-13192168 None:intergenic 45.0%
! GTTCACCCCTTCTTTTGTGT+GGG - chr7_4:13192191-13192210 None:intergenic 45.0%
! TGTTCACCCCTTCTTTTGTG+TGG - chr7_4:13192192-13192211 None:intergenic 45.0%
TCAAATTCCGACGAGTCTGT+CGG + chr7_4:13192241-13192260 Msa1140820:CDS 45.0%
GTTGTTAGCCCTGTTGATGT+TGG + chr7_4:13192289-13192308 Msa1140820:CDS 45.0%
TTGCATGCTCATATTGCACG+GGG + chr7_4:13192395-13192414 Msa1140820:CDS 45.0%
CCAATGCACGTTCTGCTTTA+TGG - chr7_4:13192462-13192481 None:intergenic 45.0%
CCATAAAGCAGAACGTGCAT+TGG + chr7_4:13192459-13192478 Msa1140820:CDS 45.0%
AACCCTAGGATTACCCCAAA+TGG + chr7_4:13192616-13192635 Msa1140820:CDS 45.0%
! GAACCATTTGGGGTAATCCT+AGG - chr7_4:13192622-13192641 None:intergenic 45.0%
AGGATTACCCCAAATGGTTC+TGG + chr7_4:13192622-13192641 Msa1140820:CDS 45.0%
!! ACCCCAAATGGTTCTGGATA+TGG + chr7_4:13192628-13192647 Msa1140820:CDS 45.0%
AATGCTGGAGTTGGAACTCT+AGG + chr7_4:13192724-13192743 Msa1140820:CDS 45.0%
GGAGTTGGAACTCTAGGTAT+CGG + chr7_4:13192730-13192749 Msa1140820:CDS 45.0%
!!! CCCTCTGATTTTGACTCTTC+TGG + chr7_4:13192861-13192880 Msa1140820:intron 45.0%
CTGGACTTGACTTAACACTC+AGG + chr7_4:13192880-13192899 Msa1140820:intron 45.0%
!! GTGGGATACACTTTTTGGTG+TGG - chr7_4:13193399-13193418 None:intergenic 45.0%
! TTATTTTCTGATGCCAGGGG+AGG + chr7_4:13193660-13193679 Msa1140820:intron 45.0%
GGTTCTACCCACACAAAAGA+AGG + chr7_4:13193792-13193811 Msa1140820:intron 45.0%
AAAAGAAGGGCTGAGCATGA+AGG + chr7_4:13193806-13193825 Msa1140820:intron 45.0%
! AGGGCATCAAAATGCCCATA+AGG + chr7_4:13192444-13192463 Msa1140820:CDS 45.0%
CAATGCACGTTCTGCCTTAT+GGG - chr7_4:13194060-13194079 None:intergenic 45.0%
!! AGGTATGATGGTGGTGCTTT+AGG + chr7_4:13194098-13194117 Msa1140820:intron 45.0%
! GATGGTGGTGCTTTAGGTTT+AGG + chr7_4:13194104-13194123 Msa1140820:intron 45.0%
CCTGAAGAGTCAATATCGGA+AGG - chr7_4:13194561-13194580 None:intergenic 45.0%
!! CCTTCCGATATTGACTCTTC+AGG + chr7_4:13194558-13194577 Msa1140820:CDS 45.0%
!! CGATATTGACTCTTCAGGGT+TGG + chr7_4:13194563-13194582 Msa1140820:CDS 45.0%
!!! TTAAATTTATTTTGAATTAA+TGG - chr7_4:13193620-13193639 None:intergenic 5.0%
!! TAGGGGCCTTGGTGTTAGAA+TGG + chr7_4:13192564-13192583 Msa1140820:intron 50.0%
GTGGGTAGTAATGCTGGAGT+TGG + chr7_4:13192715-13192734 Msa1140820:CDS 50.0%
!! GTTGGAACTCTAGGTATCGG+TGG + chr7_4:13192733-13192752 Msa1140820:CDS 50.0%
TGTGGCTACTGCTTCTGCAA+TGG + chr7_4:13192794-13192813 Msa1140820:intron 50.0%
! GCTCAGCCCTTCTTTTGTGT+GGG - chr7_4:13193802-13193821 None:intergenic 50.0%
! TGCTCAGCCCTTCTTTTGTG+TGG - chr7_4:13193803-13193822 None:intergenic 50.0%
! TTCCAGCAAGTCTGTCGCTT+CGG + chr7_4:13193857-13193876 Msa1140820:intron 50.0%
TCTACTCTCCAAGGCTTAGG+AGG + chr7_4:13194023-13194042 Msa1140820:CDS 50.0%
CTACTCTCCAAGGCTTAGGA+GGG + chr7_4:13194024-13194043 Msa1140820:CDS 50.0%
TTTGATGCCCTCCTAAGCCT+TGG - chr7_4:13194034-13194053 None:intergenic 50.0%
CCAATGCACGTTCTGCCTTA+TGG - chr7_4:13194061-13194080 None:intergenic 50.0%
CCATAAGGCAGAACGTGCAT+TGG + chr7_4:13194058-13194077 Msa1140820:CDS 50.0%
!! TAGGGGCCTTGGTGTTAGAA+TGG + chr7_4:13192564-13192583 Msa1140820:intron 50.0%
CTGGAGTTGGAACTCTAGGT+TGG + chr7_4:13192728-13192747 Msa1140820:CDS 50.0%
CAGGGTTGGACTTAACACTC+AGG + chr7_4:13194577-13194596 Msa1140820:CDS 50.0%
! TTCCGACGAGTCTGTCGGTT+CGG + chr7_4:13192246-13192265 Msa1140820:CDS 55.0%
TACCGAACCGACAGACTCGT+CGG - chr7_4:13192251-13192270 None:intergenic 55.0%
! GATGGCGGTGCTAGTGCTTT+AGG + chr7_4:13192505-13192524 Msa1140820:intron 55.0%
! TTTTCTGATGCCAGGGGAGG+AGG + chr7_4:13193663-13193682 Msa1140820:intron 55.0%
TTCCGAAGCGACAGACTTGC+TGG - chr7_4:13193862-13193881 None:intergenic 55.0%
TCTGATGCCAGGGGAGGAGG+CGG + chr7_4:13193666-13193685 Msa1140820:intron 65.0%
CTTAAACCCGCCTCCTCCCC+TGG - chr7_4:13193676-13193695 None:intergenic 65.0%
! CAGGGGAGGAGGCGGGTTTA+AGG + chr7_4:13193674-13193693 Msa1140820:intron 65.0%
! GGGAGGAGGCGGGTTTAAGG+TGG + chr7_4:13193677-13193696 Msa1140820:intron 65.0%
CTGATGCCAGGGGAGGAGGC+GGG + chr7_4:13193667-13193686 Msa1140820:intron 70.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 13192133 13194605 13192133 ID=Msa1140820;Name=Msa1140820
chr7_4 mRNA 13192133 13194605 13192133 ID=Msa1140820-mRNA-1;Parent=Msa1140820;Name=Msa1140820-mRNA-1;_AED=0.11;_eAED=0.12;_QI=0|0|0|1|0|0|6|0|412
chr7_4 exon 13192133 13192267 13192133 ID=Msa1140820-mRNA-1:exon:3151;Parent=Msa1140820-mRNA-1
chr7_4 exon 13192274 13192500 13192274 ID=Msa1140820-mRNA-1:exon:3152;Parent=Msa1140820-mRNA-1
chr7_4 exon 13192567 13192777 13192567 ID=Msa1140820-mRNA-1:exon:3153;Parent=Msa1140820-mRNA-1
chr7_4 exon 13193867 13194099 13193867 ID=Msa1140820-mRNA-1:exon:3154;Parent=Msa1140820-mRNA-1
chr7_4 exon 13194163 13194381 13194163 ID=Msa1140820-mRNA-1:exon:3155;Parent=Msa1140820-mRNA-1
chr7_4 exon 13194392 13194605 13194392 ID=Msa1140820-mRNA-1:exon:3156;Parent=Msa1140820-mRNA-1
chr7_4 CDS 13192133 13192267 13192133 ID=Msa1140820-mRNA-1:cds;Parent=Msa1140820-mRNA-1
chr7_4 CDS 13192274 13192500 13192274 ID=Msa1140820-mRNA-1:cds;Parent=Msa1140820-mRNA-1
chr7_4 CDS 13192567 13192777 13192567 ID=Msa1140820-mRNA-1:cds;Parent=Msa1140820-mRNA-1
chr7_4 CDS 13193867 13194099 13193867 ID=Msa1140820-mRNA-1:cds;Parent=Msa1140820-mRNA-1
chr7_4 CDS 13194163 13194381 13194163 ID=Msa1140820-mRNA-1:cds;Parent=Msa1140820-mRNA-1
chr7_4 CDS 13194392 13194605 13194392 ID=Msa1140820-mRNA-1:cds;Parent=Msa1140820-mRNA-1
Gene Sequence

>Msa1140820

ATGGCATCCAAATCCTTCAGCATCTCTTCTGCTTCAAAAGATGAAGGTGATTCTTCCCACACAAAAGAAGGGGTGAACATGAAGGCGAATGAAGATCAACCCTCAAAATCAAATTCCGACGAGTCTGTCGGTTCGCCATCGAAAGATGATGTTGTTAGCCCTGTTGATGTTGGTTCAACATTTTCTTTTCCTAATAACAGCAATGAAGGGAAAGATGAGAAGAAGAACGAAGAGAAGAACTCGGATGTGAAGTATTTTGCATGCTCATATTGCACGGGGCAATATTCTACTTTACAAGGGTTAAGAGGGCATCAAAATGCCCATAAAGCAGAACGTGCATTGGAAAAACAACGTAGAAAAAGGGGCCTTGGTGTTAGAATGGAGTCAACAATTCAAAAAACACCTTATACTAACCCTAGGATTACCCCAAATGGTTCTGGATATGGTTATGGTGCTTTGCGTTTGAATGATGTATTACACCCTTCACTTTTTAATTTGAGAAATAATATTGTGGGTAGTAATGCTGGAGTTGGAACTCTAGGTATCGGTGGAGCAAGTACTTCAAGAATTGAATCTGTCGCTTCGGAAAAGATATCGAAAGATGATGTTGTTAGCCCTATTCAAGTTGGTTCAACATCTTCTTTTCCTAATAACAGCAATGAAGGGAAAGATGAGAAGAAGAATGAAGAGAAGAAGTATTTTACATGCTCGTATTGCAAGGGGAAATTTTCTACTCTCCAAGGCTTAGGAGGGCATCAAAATGCCCATAAGGCAGAACGTGCATTGGAAAAACAACGTAAAGAAAGTACTCGTTTTACACCTTATAATAATTATAGGGGCCTTGGTGTTAGAATGGAGTTAACAATTCAAAAACCACCTTATACTAACCCTAGGTTTATCCCAAATGGTTCTAGATATGGTTATGGTGCTTTGCGTTTGAATGATGTATTACACCCTTCACTTTTTAACTTGAGAAATAATATTATGGGTAGTAATACTGGAGTTGGAACTCTAGGTTGGAGCAAAATTGAAGTTGGTACAAATAATCAAATTGGTGCTATTCTAAAACTTGGAGATTCTTCTACTGCTACAAGTTCAAGCTCAAAGACAGACAAGAAAAATGTTGTGGCTACTACTTCTACAACGGATGATGATACTGATCATGATTCAAAATCAAGCATTGAAGAAGAACCTTCCGATATTGACTCTTCAGGGTTGGACTTAACACTCAGGCTTTAG
Protein sequence

>Msa1140820

MASKSFSISSASKDEGDSSHTKEGVNMKANEDQPSKSNSDESVGSPSKDDVVSPVDVGSTFSFPNNSNEGKDEKKNEEKNSDVKYFACSYCTGQYSTLQGLRGHQNAHKAERALEKQRRKRGLGVRMESTIQKTPYTNPRITPNGSGYGYGALRLNDVLHPSLFNLRNNIVGSNAGVGTLGIGGASTSRIESVASEKISKDDVVSPIQVGSTSSFPNNSNEGKDEKKNEEKKYFTCSYCKGKFSTLQGLGGHQNAHKAERALEKQRKESTRFTPYNNYRGLGVRMELTIQKPPYTNPRFIPNGSRYGYGALRLNDVLHPSLFNLRNNIMGSNTGVGTLGWSKIEVGTNNQIGAILKLGDSSTATSSSSKTDKKNVVATTSTTDDDTDHDSKSSIEEEPSDIDSSGLDLTLRL*