AlfalfaGEDB | Msa1141160

M. sativa cultivar zhongmu-4 / Msa1141160

KEGG: K14491
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1141160	AES78253.1	86.364	374	42	2	1	366	69	441	0.0	608

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1141160	sp\|Q9ZWJ9\|ARR2_ARATH	37.989	179	83	5	1	157	106	278	1.14e-25	112

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1141160	G7L033	86.364	374	42	2	1	366	69	441	0.0	608

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TFs/TRs:

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Gene ID	Type	Classification
Msa1141160	TF	GARP-G2-like

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1141160	MtrunA17_Chr7g0223971	79.115	407	43	3	1	366	1	406	0.0	589
Msa1141160	MtrunA17_Chr7g0223991	70.233	215	35	5	156	360	24	219	1.51e-90	272
Msa1141160	MtrunA17_Chr6g0473731	45.430	372	145	10	1	357	12	340	1.33e-79	251
Msa1141160	MtrunA17_Chr4g0067981	36.478	159	87	1	1	145	113	271	7.91e-26	109
Msa1141160	MtrunA17_Chr2g0294911	38.750	160	86	4	1	148	111	270	5.91e-25	106
Msa1141160	MtrunA17_Chr4g0009981	28.862	246	129	7	1	229	39	255	1.21e-24	101
Msa1141160	MtrunA17_Chr1g0161431	31.771	192	106	5	1	168	93	283	3.79e-24	104
Msa1141160	MtrunA17_Chr4g0009851	31.799	239	131	9	1	229	92	308	6.16e-24	100
Msa1141160	MtrunA17_Chr1g0149841	34.118	170	82	3	2	148	88	250	5.46e-23	100
Msa1141160	MtrunA17_Chr8g0376881	33.690	187	106	5	1	169	107	293	6.05e-23	100

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1141160	AT3G16857.3	32.218	239	112	6	1	195	45	277	1.59e-26	111
Msa1141160	AT3G16857.1	35.079	191	84	4	1	157	115	299	3.26e-26	110
Msa1141160	AT3G16857.2	35.079	191	84	4	1	157	115	299	3.90e-26	110
Msa1141160	AT5G07210.2	60.465	86	34	0	80	165	213	298	2.12e-25	108
Msa1141160	AT5G07210.1	60.465	86	34	0	80	165	221	306	2.14e-25	108
Msa1141160	AT4G31920.1	34.211	152	100	0	1	152	95	246	1.81e-22	99.4
Msa1141160	AT2G01760.3	31.214	173	91	3	1	148	25	194	9.88e-22	95.1
Msa1141160	AT2G25180.1	35.625	160	91	2	1	148	95	254	1.48e-21	97.1
Msa1141160	AT2G27070.1	43.609	133	71	2	37	165	169	301	4.41e-21	95.5
Msa1141160	AT2G01760.1	31.214	173	91	3	1	148	90	259	4.64e-21	94.0

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Find 61 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATAACAATGAAACGGGGA+GGG	0.644017	7_4:-14204690	Msa1141160:CDS
AAATGTAGCAAGTCACTTAC+AGG	0.277878	7_4:-14205550	Msa1141160:intron
AACTATGAACAATGGATTAA+GGG	0.373260	7_4:-14204723	Msa1141160:CDS
AATAATGGAGCAGGAATGGT+TGG	0.529804	7_4:-14204752	Msa1141160:CDS
AATTGGTCAACATGATGTGT+TGG	0.569003	7_4:+14205139	None:intergenic
ACAATGAAACGGGGAGGGAG+TGG	0.514140	7_4:-14204685	Msa1141160:CDS
ACACTGGCATATCAAACAAG+AGG	0.654807	7_4:-14205196	Msa1141160:CDS
ACATATTGGGAAATATGTTA+TGG	0.400575	7_4:-14206523	Msa1141160:CDS
ACATCAGAAGTAAATTTATG+AGG	0.469959	7_4:+14204806	None:intergenic
ACTTACTTTCCAATCCAATT+TGG	0.301438	7_4:+14206298	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTGAAGACTTCAAACATATT+GGG	-	chr7_4:14204740-14204759	Msa1141160:CDS	30.0%
AGAAAGAATAAAGAATGCCA+AGG	-	chr7_4:14204874-14204893	Msa1141160:CDS	30.0%
ACTTACTTTCCAATCCAATT+TGG	+	chr7_4:14204981-14205000	None:intergenic	30.0%
TACTTCTTCCAACCATTAAT+CGG	-	chr7_4:14205071-14205090	Msa1141160:CDS	30.0%
TTTCATTGTTGGATTGATAG+TGG	+	chr7_4:14205151-14205170	None:intergenic	30.0%
CCTCAAGTATATTTCATTGT+TGG	+	chr7_4:14205162-14205181	None:intergenic	30.0%
CCAACAATGAAATATACTTG+AGG	-	chr7_4:14205159-14205178	Msa1141160:CDS	30.0%
CATTTGACAACCATATCATA+TGG	-	chr7_4:14205250-14205269	Msa1141160:CDS	30.0%
ACACCACTATCATTGTTTAT+AGG	-	chr7_4:14205341-14205360	Msa1141160:CDS	30.0%
ATAACAGCTATGATCAAAAC+AGG	-	chr7_4:14206249-14206268	Msa1141160:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	14204658	14206640	14204658	ID=Msa1141160;Name=Msa1141160
chr7_4	mRNA	14204658	14206640	14204658	ID=Msa1141160-mRNA-1;Parent=Msa1141160;Name=Msa1141160-mRNA-1;_AED=0.12;_eAED=0.16;_QI=0\|0\|0\|1\|1\|1\|3\|0\|380
chr7_4	exon	14206304	14206640	14206304	ID=Msa1141160-mRNA-1:exon:3334;Parent=Msa1141160-mRNA-1
chr7_4	exon	14205551	14205627	14205551	ID=Msa1141160-mRNA-1:exon:3333;Parent=Msa1141160-mRNA-1
chr7_4	exon	14204658	14205386	14204658	ID=Msa1141160-mRNA-1:exon:3332;Parent=Msa1141160-mRNA-1
chr7_4	CDS	14206304	14206640	14206304	ID=Msa1141160-mRNA-1:cds;Parent=Msa1141160-mRNA-1
chr7_4	CDS	14205551	14205627	14205551	ID=Msa1141160-mRNA-1:cds;Parent=Msa1141160-mRNA-1
chr7_4	CDS	14204658	14205386	14204658	ID=Msa1141160-mRNA-1:cds;Parent=Msa1141160-mRNA-1

Gene Sequence

>Msa1141160

ATGTCTGAAGACAATAGGCCAAGTTTAATCTCGAAGACACTAGAAAATGGAGCTGCACAGTACATTGTGAAACCTTTTTGTGCTGAAGACTTCAAACATATTGGGAAATATGTTATGGAAGCAAAGAAAGAGAAACTTTTCATTGAAAGTTTATTTGTGAGAAGCGAAGAAGAAGAGATATCAAGTGAACCTAAGAACGAAAAGAAGCATTGCAAGAGAAAGAATAAAGAATGCCAAGGAGAGAGAGATATTCAAGTTGTTAAGAAACAAAAGCTTGTTTGGACACCTTATCTCCACAAAATGTTTCTGCTTGCTATTGACCAAATTGGATTGGAAAAGGCTGTGCCAAAGAAAATTCTGGAGATCATGAATATACCTAACGTGACCAGAGAAAATGTAGCAAGTCACTTACAGAAATATCGCATATTTCTGCGAGATGTTGCAGAGAAAGGAATGGTTGGAGGCATATCTCACAGAGCTCTAAGATCAAGGTTTGCATCAAGTCTTCCGATGTCTATGATAAGAGAAATCGAAGAAAAAAGAACAAACAAACTTCATGCGCCAACACTTCCATATTTGCAAACACTGGCATATCAAACAAGAGGTGCAAACAATGTTTTTAACCATTTCAACCAATTTCCAACACATCATGTTGACCAATTTCCTTATATACAAAAAGGGCTGGCTCTTCAAGATTTGGAACAAGTTAGGCTTGGAAAATCAAAGTTGGAAGCCGATGTTGTTGCAGAGAATAACAGCTATGATCAAAACAGGTTTGGAATAAGCTCTTCAAGCCTTCCAATTTTTCAAGCGAAAAACTCATTCAATGATGGTGCTTCCACTAGCTTTTCATCATATAGAGCTGAACAAGGATTGATGGTTTCTTCTTCTTCTTCAAGTTTCTTAACAAGGGATTTTAAATTTGGTGATATTAGAAACCAAAATTATGGTGTTAAAAGCACTAATGTCATGCCTCATAAATTTACTTCTGATGTAAGAAATAACAATCCTTTTGGAATTCAGCTGAATAATGGAGCAGGAATGGTTGGAATTGGAACTATGAACAATGGATTAAGGGCTTCTAGTCCAAATAACAATGAAACGGGGAGGGAGTGGAAGAGAGTTTCTAGCAGGATCAGTTAA

Protein sequence

>Msa1141160

MSEDNRPSLISKTLENGAAQYIVKPFCAEDFKHIGKYVMEAKKEKLFIESLFVRSEEEEISSEPKNEKKHCKRKNKECQGERDIQVVKKQKLVWTPYLHKMFLLAIDQIGLEKAVPKKILEIMNIPNVTRENVASHLQKYRIFLRDVAEKGMVGGISHRALRSRFASSLPMSMIREIEEKRTNKLHAPTLPYLQTLAYQTRGANNVFNHFNQFPTHHVDQFPYIQKGLALQDLEQVRLGKSKLEADVVAENNSYDQNRFGISSSSLPIFQAKNSFNDGASTSFSSYRAEQGLMVSSSSSSFLTRDFKFGDIRNQNYGVKSTNVMPHKFTSDVRNNNPFGIQLNNGAGMVGIGTMNNGLRASSPNNNETGREWKRVSSRIS*