AlfalfaGEDB | Msa1146400

M. sativa cultivar zhongmu-4 / Msa1146400

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009909 GO:0010048 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048580 GO:0048831 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0065007 GO:0070013 GO:0097159 GO:1901363 GO:2000026 GO:2000241
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1146400	AES78883.1	91.118	304	26	1	1	304	1	303	0.0	549

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1146400	sp\|Q9ZSH7\|Y4158_ARATH	43.269	104	59	0	13	116	30	133	1.38e-21	93.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1146400	G7KVV6	91.118	304	26	1	1	304	1	303	0.0	549

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TFs/TRs:

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Gene ID	Type	Classification
Msa1146400	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1146400	MtrunA17_Chr7g0233531	91.459	281	23	1	28	308	1	280	0.0	506
Msa1146400	MtrunA17_Chr7g0233561	71.692	325	74	4	1	308	6	329	3.46e-163	457
Msa1146400	MtrunA17_Chr3g0131891	69.524	315	88	3	1	308	7	320	8.34e-159	446
Msa1146400	MtrunA17_Chr7g0233541	69.231	299	72	6	28	308	1	297	1.04e-142	404
Msa1146400	MtrunA17_Chr7g0233521	65.993	297	68	8	28	308	1	280	3.54e-130	371
Msa1146400	MtrunA17_Chr7g0233571	57.674	215	70	6	7	202	4	216	5.89e-76	231
Msa1146400	MtrunA17_Chr8g0390841	35.016	317	148	9	11	291	37	331	6.87e-47	165
Msa1146400	MtrunA17_Chr8g0390841	25.784	287	195	10	13	291	242	518	1.01e-13	71.6
Msa1146400	MtrunA17_Chr1g0208771	33.333	303	151	9	29	298	13	297	1.88e-44	157
Msa1146400	MtrunA17_Chr1g0163071	33.007	306	158	8	28	298	1	294	5.24e-43	150

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1146400	AT3G18990.2	26.608	342	183	7	13	294	6	339	2.75e-30	118
Msa1146400	AT4G01580.1	43.269	104	59	0	13	116	30	133	1.40e-22	93.2
Msa1146400	AT1G49475.1	40.517	116	68	1	13	128	34	148	3.45e-22	92.0
Msa1146400	AT4G33280.1	27.586	319	185	14	11	298	23	326	5.36e-22	94.7
Msa1146400	AT3G18960.2	34.211	152	84	2	13	148	30	181	2.31e-21	90.1
Msa1146400	AT3G18990.1	41.414	99	58	0	13	111	6	104	4.02e-21	92.4
Msa1146400	AT3G18960.1	34.211	152	84	2	13	148	30	181	4.34e-21	89.7
Msa1146400	AT3G18960.3	35.294	136	72	2	29	148	9	144	7.32e-18	80.1
Msa1146400	AT3G53310.1	33.333	102	63	2	3	104	5	101	6.44e-12	65.5

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Find 40 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTAACTCCTTTGAATT+TGG	0.175508	7_4:+23940542	Msa1146400:CDS
AAAGGCTTACAAGCTTTCCT+TGG	0.474678	7_4:-23939415	None:intergenic
AAATCACAAAAGAAGTCCAA+AGG	0.633010	7_4:+23940590	Msa1146400:CDS
AATTTGATATCACAAGTGGA+TGG	0.625506	7_4:+23941321	Msa1146400:CDS
ACATGTCTAAAGACTCCAAA+TGG	0.505252	7_4:+23940251	Msa1146400:CDS
ACTACTACTTCCAAATTCAA+AGG	0.556173	7_4:-23940552	None:intergenic
AGAAAATTTGTGGAGAAATA+TGG	0.268462	7_4:+23940209	Msa1146400:CDS
AGACTCCAAATGGTGGAAAA+TGG	0.283126	7_4:+23940261	Msa1146400:CDS
AGATTCATTTCCTTGATCAA+TGG	0.441958	7_4:-23940483	None:intergenic
AGGTATGGTTTGAAAAGGGT+TGG	0.528620	7_4:+23940312	Msa1146400:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATGACTCAATTGCTTTC+AGG	-	chr7_4:23939641-23939660	None:intergenic	30.0%
AAAAATGATGGTTGCTCTTT+AGG	+	chr7_4:23939736-23939755	Msa1146400:intron	30.0%
ATGAAAAAATGAAGGGTTTC+TGG	+	chr7_4:23939972-23939991	Msa1146400:intron	30.0%
CATATCTAAAAAGCAGAAGA+TGG	-	chr7_4:23940373-23940392	None:intergenic	30.0%
ATATGTACCTCAAAATTGGA+AGG	-	chr7_4:23940401-23940420	None:intergenic	30.0%
AAAAAGATCTCCATTGATCA+AGG	+	chr7_4:23940473-23940492	Msa1146400:CDS	30.0%
AGATTCATTTCCTTGATCAA+TGG	-	chr7_4:23940486-23940505	None:intergenic	30.0%
CAAGGAAATGAATCTCTAAA+TGG	+	chr7_4:23940491-23940510	Msa1146400:CDS	30.0%
AAAGCTAACTCCTTTGAATT+TGG	+	chr7_4:23940542-23940561	Msa1146400:CDS	30.0%
ACTACTACTTCCAAATTCAA+AGG	-	chr7_4:23940555-23940574	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	23939347	23941496	23939347	ID=Msa1146400;Name=Msa1146400
chr7_4	mRNA	23939347	23941496	23939347	ID=Msa1146400-mRNA-1;Parent=Msa1146400;Name=Msa1146400-mRNA-1;_AED=0.08;_eAED=0.09;_QI=0\|0\|0\|1\|1\|0.75\|4\|0\|308
chr7_4	exon	23939347	23939427	23939347	ID=Msa1146400-mRNA-1:exon:5996;Parent=Msa1146400-mRNA-1
chr7_4	exon	23940200	23940611	23940200	ID=Msa1146400-mRNA-1:exon:5997;Parent=Msa1146400-mRNA-1
chr7_4	exon	23940755	23940882	23940755	ID=Msa1146400-mRNA-1:exon:5998;Parent=Msa1146400-mRNA-1
chr7_4	exon	23941191	23941496	23941191	ID=Msa1146400-mRNA-1:exon:5999;Parent=Msa1146400-mRNA-1
chr7_4	CDS	23939347	23939427	23939347	ID=Msa1146400-mRNA-1:cds;Parent=Msa1146400-mRNA-1
chr7_4	CDS	23940200	23940611	23940200	ID=Msa1146400-mRNA-1:cds;Parent=Msa1146400-mRNA-1
chr7_4	CDS	23940755	23940882	23940755	ID=Msa1146400-mRNA-1:cds;Parent=Msa1146400-mRNA-1
chr7_4	CDS	23941191	23941496	23941191	ID=Msa1146400-mRNA-1:cds;Parent=Msa1146400-mRNA-1

Gene Sequence

>Msa1146400

ATGGCTAGTGAAATTTATGAGGTGAAGCCAATTCACTTTTTCAAGATCATACTTGCTCAAAACCTTCACCAAGGAAAGCTTATGATGCCAAGAAAATTTGTGGAGAAATATGGAAAAAGTCTACCAAAAGAAACATGTCTAAAGACTCCAAATGGTGGAAAATGGAAATTAAATTTGGTAAAAAGTGATGGCAAGGTATGGTTTGAAAAGGGTTGGAAAGAATTTGCAGAGCATCACTCTCTAGCTCATGGCCATCTTCTGCTTTTTAGATATGAAAAACCTTCCAATTTTGAGGTACATATTTTTGAAAAGAGTGCGTTGGAGACAAATTACCCTACCAAAAGAGTTGAAGATAAAAAGATCTCCATTGATCAAGGAAATGAATCTCTAAATGGTGAAAATTGTAGAGCAGCTCAAAAGAGAAAAGCTAACTCCTTTGAATTTGGAAGTAGTAGTTGTGTTAAAGTTGGGAAATCACAAAAGAAGTCCAAAGGAAAACAAGTGATTACTGCTGCTGAAAAAGTTACAACTCTTGAAAGAGCAGAATCTTTCAAAATAATAAATCCATCATTTGTTGTTGTTGTCATGCGTGCATCATATGTTGAACATAGATTTCTGATGAATATACCACGTATGTTTGGTAATAGACACTTTGATTTGGATAAGAAAAGAGGAGATATTTATTTTCAGGTGTTAAATGAAGGAGTTTGGCCTACAAGGTTTTCTATCAGGAAGTCCAGTACAGGACTACAATTTGATATCACAAGTGGATGGAAGGCATTTGCAGAGGACAATAACTTGAAAGTTGGTGATGTTTGCAAATTTGAGCTCATCTCTAGCACTACACTTACCTTCATTGTTCATATCTTTAGAAATACAGACAATGACAACTCAAAGTGTTCAACTTCTCAAAGTAAGATCAATTGA

Protein sequence

>Msa1146400

MASEIYEVKPIHFFKIILAQNLHQGKLMMPRKFVEKYGKSLPKETCLKTPNGGKWKLNLVKSDGKVWFEKGWKEFAEHHSLAHGHLLLFRYEKPSNFEVHIFEKSALETNYPTKRVEDKKISIDQGNESLNGENCRAAQKRKANSFEFGSSSCVKVGKSQKKSKGKQVITAAEKVTTLERAESFKIINPSFVVVVMRASYVEHRFLMNIPRMFGNRHFDLDKKRGDIYFQVLNEGVWPTRFSIRKSSTGLQFDITSGWKAFAEDNNLKVGDVCKFELISSTTLTFIVHIFRNTDNDNSKCSTSQSKIN*