Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1147840 | AES79096.1 | 66.355 | 107 | 9 | 2 | 1 | 107 | 1 | 80 | 1.05e-37 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1147840 | sp|Q9FJK3|AGL80_ARATH | 52.830 | 106 | 49 | 1 | 1 | 105 | 1 | 106 | 9.42e-27 | 103 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1147840 | G7L067 | 66.355 | 107 | 9 | 2 | 1 | 107 | 1 | 80 | 5.01e-38 | 132 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1147840 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1147840 | MtrunA17_Chr4g0014321 | 59.259 | 108 | 43 | 1 | 1 | 107 | 1 | 108 | 1.40e-36 | 124 |
| Msa1147840 | MtrunA17_Chr8g0351751 | 61.386 | 101 | 36 | 1 | 1 | 98 | 1 | 101 | 4.62e-36 | 122 |
| Msa1147840 | MtrunA17_Chr4g0014031 | 62.037 | 108 | 40 | 1 | 1 | 107 | 1 | 108 | 1.42e-35 | 121 |
| Msa1147840 | MtrunA17_Chr2g0296831 | 63.889 | 108 | 38 | 1 | 1 | 107 | 1 | 108 | 2.51e-35 | 117 |
| Msa1147840 | MtrunA17_Chr3g0107381 | 52.778 | 108 | 50 | 1 | 1 | 107 | 1 | 108 | 2.59e-34 | 117 |
| Msa1147840 | MtrunA17_Chr3g0110131 | 54.630 | 108 | 48 | 1 | 1 | 107 | 1 | 108 | 5.18e-32 | 112 |
| Msa1147840 | MtrunA17_Chr4g0013041 | 51.852 | 108 | 49 | 2 | 1 | 107 | 1 | 106 | 6.29e-30 | 106 |
| Msa1147840 | MtrunA17_Chr4g0014121 | 58.333 | 108 | 44 | 1 | 1 | 107 | 29 | 136 | 2.94e-29 | 103 |
| Msa1147840 | MtrunA17_Chr4g0014131 | 58.333 | 108 | 44 | 1 | 1 | 107 | 1 | 108 | 4.52e-29 | 104 |
| Msa1147840 | MtrunA17_Chr3g0107331 | 45.283 | 106 | 57 | 1 | 3 | 107 | 2 | 107 | 2.99e-28 | 100 |
| Msa1147840 | MtrunA17_Chr3g0107461 | 43.137 | 102 | 57 | 1 | 1 | 101 | 1 | 102 | 1.07e-24 | 91.7 |
| Msa1147840 | MtrunA17_Chr3g0107361 | 42.453 | 106 | 60 | 1 | 3 | 107 | 2 | 107 | 4.17e-24 | 90.1 |
| Msa1147840 | MtrunA17_Chr3g0107341 | 43.396 | 106 | 59 | 1 | 3 | 107 | 2 | 107 | 7.64e-24 | 89.4 |
| Msa1147840 | MtrunA17_Chr5g0420351 | 42.157 | 102 | 59 | 0 | 1 | 102 | 1 | 102 | 2.08e-23 | 88.2 |
| Msa1147840 | MtrunA17_Chr3g0107351 | 41.509 | 106 | 61 | 1 | 3 | 107 | 2 | 107 | 3.51e-23 | 87.8 |
| Msa1147840 | MtrunA17_Chr1g0188661 | 43.925 | 107 | 60 | 0 | 1 | 107 | 1 | 107 | 1.85e-22 | 85.9 |
| Msa1147840 | MtrunA17_Chr7g0218151 | 44.860 | 107 | 59 | 0 | 1 | 107 | 1 | 107 | 2.37e-22 | 85.5 |
| Msa1147840 | MtrunA17_Chr1g0197261 | 37.383 | 107 | 67 | 0 | 1 | 107 | 1 | 107 | 1.85e-21 | 83.2 |
| Msa1147840 | MtrunA17_Chr1g0188681 | 39.252 | 107 | 65 | 0 | 1 | 107 | 1 | 107 | 2.60e-21 | 82.8 |
| Msa1147840 | MtrunA17_Chr4g0012761 | 42.991 | 107 | 61 | 0 | 1 | 107 | 1 | 107 | 4.20e-21 | 82.4 |
| Msa1147840 | MtrunA17_Chr2g0283941 | 41.284 | 109 | 61 | 2 | 1 | 107 | 1 | 108 | 9.80e-21 | 83.2 |
| Msa1147840 | MtrunA17_Chr1g0197461 | 39.000 | 100 | 61 | 0 | 3 | 102 | 2 | 101 | 1.03e-20 | 81.3 |
| Msa1147840 | MtrunA17_Chr5g0432691 | 37.963 | 108 | 66 | 1 | 1 | 107 | 1 | 108 | 2.91e-20 | 80.1 |
| Msa1147840 | MtrunA17_Chr1g0193311 | 42.056 | 107 | 62 | 0 | 1 | 107 | 1 | 107 | 4.29e-20 | 79.7 |
| Msa1147840 | MtrunA17_Chr1g0197321 | 35.922 | 103 | 64 | 2 | 1 | 102 | 1 | 102 | 1.71e-18 | 75.5 |
| Msa1147840 | MtrunA17_Chr1g0188651 | 43.137 | 102 | 58 | 0 | 1 | 102 | 1 | 102 | 5.08e-18 | 74.3 |
| Msa1147840 | MtrunA17_Chr3g0105791 | 44.872 | 78 | 42 | 1 | 29 | 105 | 1 | 78 | 1.07e-17 | 73.2 |
| Msa1147840 | MtrunA17_Chr4g0031421 | 42.857 | 77 | 43 | 1 | 29 | 104 | 1 | 77 | 3.09e-17 | 72.0 |
| Msa1147840 | MtrunA17_Chr2g0296861 | 34.737 | 95 | 61 | 1 | 1 | 94 | 1 | 95 | 5.02e-16 | 69.3 |
| Msa1147840 | MtrunA17_Chr7g0267601 | 36.036 | 111 | 67 | 3 | 1 | 107 | 1 | 111 | 2.39e-14 | 67.4 |
| Msa1147840 | MtrunA17_Chr4g0008701 | 36.937 | 111 | 66 | 3 | 1 | 107 | 1 | 111 | 4.99e-14 | 66.6 |
| Msa1147840 | MtrunA17_Chr3g0106421 | 37.838 | 74 | 45 | 1 | 31 | 103 | 3 | 76 | 1.02e-11 | 58.9 |
| Msa1147840 | MtrunA17_Chr3g0128721 | 30.841 | 107 | 71 | 2 | 4 | 107 | 5 | 111 | 4.73e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1147840 | AT5G48670.1 | 52.830 | 106 | 49 | 1 | 1 | 105 | 1 | 106 | 9.58e-28 | 103 |
| Msa1147840 | AT3G05860.2 | 43.617 | 94 | 52 | 1 | 1 | 93 | 1 | 94 | 2.59e-23 | 89.4 |
| Msa1147840 | AT3G05860.3 | 43.617 | 94 | 52 | 1 | 1 | 93 | 1 | 94 | 1.04e-22 | 89.0 |
| Msa1147840 | AT3G05860.1 | 43.617 | 94 | 52 | 1 | 1 | 93 | 1 | 94 | 1.41e-22 | 88.6 |
| Msa1147840 | AT5G27810.1 | 46.250 | 80 | 42 | 1 | 29 | 107 | 1 | 80 | 2.25e-21 | 82.0 |
| Msa1147840 | AT1G65300.1 | 42.453 | 106 | 60 | 1 | 3 | 107 | 2 | 107 | 5.68e-21 | 84.7 |
| Msa1147840 | AT1G65330.1 | 40.566 | 106 | 62 | 1 | 3 | 107 | 2 | 107 | 8.52e-20 | 81.6 |
| Msa1147840 | AT5G27960.1 | 41.414 | 99 | 57 | 1 | 4 | 101 | 2 | 100 | 1.68e-18 | 79.0 |
| Msa1147840 | AT5G26650.1 | 41.414 | 99 | 57 | 1 | 4 | 101 | 2 | 100 | 5.43e-18 | 77.8 |
| Msa1147840 | AT5G26630.1 | 42.593 | 108 | 61 | 1 | 1 | 107 | 1 | 108 | 1.91e-17 | 74.3 |
| Msa1147840 | AT1G31630.1 | 33.962 | 106 | 68 | 2 | 3 | 107 | 2 | 106 | 2.31e-15 | 70.5 |
| Msa1147840 | AT5G26580.1 | 36.190 | 105 | 48 | 2 | 1 | 102 | 1 | 89 | 3.09e-15 | 70.1 |
| Msa1147840 | AT2G28700.1 | 33.981 | 103 | 67 | 1 | 1 | 102 | 1 | 103 | 8.60e-15 | 68.6 |
| Msa1147840 | AT1G22590.2 | 33.333 | 105 | 65 | 2 | 1 | 102 | 1 | 103 | 3.26e-14 | 65.1 |
| Msa1147840 | AT1G31640.1 | 32.075 | 106 | 70 | 2 | 3 | 107 | 2 | 106 | 4.57e-12 | 61.2 |
| Msa1147840 | AT5G55690.1 | 46.269 | 67 | 26 | 2 | 1 | 59 | 1 | 65 | 6.16e-12 | 60.5 |
| Msa1147840 | AT5G55690.2 | 46.269 | 67 | 26 | 2 | 1 | 59 | 1 | 65 | 6.16e-12 | 60.5 |
| Msa1147840 | AT5G58890.1 | 38.655 | 119 | 58 | 4 | 1 | 106 | 1 | 117 | 8.57e-12 | 60.1 |
Find 20 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTAGCATCATTTACATTAG+AGG | 0.304340 | 7_4:-27358748 | None:intergenic |
| GTTGGATAGTTACAAGAAAA+TGG | 0.330675 | 7_4:+27358947 | Msa1147840:CDS |
| ATGAACCACAACTTGAGATT+TGG | 0.331935 | 7_4:+27358874 | Msa1147840:CDS |
| TTCTACATATTGTATTTCCT+TGG | 0.356053 | 7_4:-27359029 | None:intergenic |
| ATCTCAAGTTGTGGTTCATA+AGG | 0.360688 | 7_4:-27358870 | None:intergenic |
| ATAGCACTTTGAACTTCCTT+TGG | 0.406725 | 7_4:-27358903 | None:intergenic |
| CATGAAACATAGTGTTATGA+TGG | 0.436074 | 7_4:+27358986 | Msa1147840:CDS |
| TAAAAGCAACATACAAGAAA+AGG | 0.455660 | 7_4:+27358772 | Msa1147840:CDS |
| GATGAAATAAGCACCCTTTG+TGG | 0.460320 | 7_4:+27358819 | Msa1147840:CDS |
| TATGTTTCATGAAAGTCTCT+TGG | 0.462242 | 7_4:-27358974 | None:intergenic |
| TATCCAACTCATGTATAGTC+TGG | 0.466467 | 7_4:-27358932 | None:intergenic |
| TCTCAAGTTGTGGTTCATAA+GGG | 0.506587 | 7_4:-27358869 | None:intergenic |
| CTTTGAACTTCCTTTGGCGA+TGG | 0.511371 | 7_4:-27358897 | None:intergenic |
| AATCCAGACTATACATGAGT+TGG | 0.520722 | 7_4:+27358929 | Msa1147840:CDS |
| ACAAGCTTCAATTCCACAAA+GGG | 0.520783 | 7_4:-27358832 | None:intergenic |
| TGAGATTTGGCCATCGCCAA+AGG | 0.569018 | 7_4:+27358887 | Msa1147840:CDS |
| AAGAACAAGTTAAGAAGCCA+AGG | 0.574148 | 7_4:+27359012 | Msa1147840:CDS |
| GAAGCTTGTGCTATAGTGTA+TGG | 0.585884 | 7_4:+27358846 | Msa1147840:CDS |
| CACAAGCTTCAATTCCACAA+AGG | 0.628500 | 7_4:-27358833 | None:intergenic |
| GATGGCCAAATCTCAAGTTG+TGG | 0.647253 | 7_4:-27358879 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAAAGCAACATACAAGAAA+AGG | + | chr7_4:27358772-27358791 | Msa1147840:CDS | 25.0% |
| ACTAGCATCATTTACATTAG+AGG | - | chr7_4:27358751-27358770 | None:intergenic | 30.0% | |
| GTTGGATAGTTACAAGAAAA+TGG | + | chr7_4:27358947-27358966 | Msa1147840:CDS | 30.0% | |
| TATGTTTCATGAAAGTCTCT+TGG | - | chr7_4:27358977-27358996 | None:intergenic | 30.0% | |
| CATGAAACATAGTGTTATGA+TGG | + | chr7_4:27358986-27359005 | Msa1147840:CDS | 30.0% | |
| ACAAGCTTCAATTCCACAAA+GGG | - | chr7_4:27358835-27358854 | None:intergenic | 35.0% | |
| ! | TCTCAAGTTGTGGTTCATAA+GGG | - | chr7_4:27358872-27358891 | None:intergenic | 35.0% |
| ! | ATCTCAAGTTGTGGTTCATA+AGG | - | chr7_4:27358873-27358892 | None:intergenic | 35.0% |
| ATGAACCACAACTTGAGATT+TGG | + | chr7_4:27358874-27358893 | Msa1147840:CDS | 35.0% | |
| ATAGCACTTTGAACTTCCTT+TGG | - | chr7_4:27358906-27358925 | None:intergenic | 35.0% | |
| AATCCAGACTATACATGAGT+TGG | + | chr7_4:27358929-27358948 | Msa1147840:CDS | 35.0% | |
| TATCCAACTCATGTATAGTC+TGG | - | chr7_4:27358935-27358954 | None:intergenic | 35.0% | |
| AAGAACAAGTTAAGAAGCCA+AGG | + | chr7_4:27359012-27359031 | Msa1147840:CDS | 35.0% | |
| GATGAAATAAGCACCCTTTG+TGG | + | chr7_4:27358819-27358838 | Msa1147840:CDS | 40.0% | |
| CACAAGCTTCAATTCCACAA+AGG | - | chr7_4:27358836-27358855 | None:intergenic | 40.0% | |
| GAAGCTTGTGCTATAGTGTA+TGG | + | chr7_4:27358846-27358865 | Msa1147840:CDS | 40.0% | |
| ! | GATGGCCAAATCTCAAGTTG+TGG | - | chr7_4:27358882-27358901 | None:intergenic | 45.0% |
| CTTTGAACTTCCTTTGGCGA+TGG | - | chr7_4:27358900-27358919 | None:intergenic | 45.0% | |
| TGAGATTTGGCCATCGCCAA+AGG | + | chr7_4:27358887-27358906 | Msa1147840:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 27358723 | 27359049 | 27358723 | ID=Msa1147840;Name=Msa1147840 |
| chr7_4 | mRNA | 27358723 | 27359049 | 27358723 | ID=Msa1147840-mRNA-1;Parent=Msa1147840;Name=Msa1147840-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|108 |
| chr7_4 | exon | 27358723 | 27359049 | 27358723 | ID=Msa1147840-mRNA-1:exon:6708;Parent=Msa1147840-mRNA-1 |
| chr7_4 | CDS | 27358723 | 27359049 | 27358723 | ID=Msa1147840-mRNA-1:cds;Parent=Msa1147840-mRNA-1 |
| Gene Sequence |
| Protein sequence |
