AlfalfaGEDB | Msa1147850

M. sativa cultivar zhongmu-4 / Msa1147850

KEGG: K09260
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042995 GO:0043076 GO:0043078 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090406 GO:0097159 GO:0120025 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1147850	AES79096.1	69.444	72	9	1	1	72	1	59	1.57e-24	97.8

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1147850	sp\|Q9FJK3\|AGL80_ARATH	60.000	65	26	0	1	65	1	65	2.32e-14	68.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1147850	G7L067	69.444	72	9	1	1	72	1	59	7.48e-25	97.8

TFs/TRs:

Gene ID	Type	Classification
Msa1147850	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1147850	MtrunA17_Chr8g0351751	70.588	68	20	0	1	68	1	68	5.60e-27	98.2
Msa1147850	MtrunA17_Chr4g0014321	67.647	68	22	0	1	68	1	68	1.63e-24	91.3
Msa1147850	MtrunA17_Chr4g0014031	70.588	68	20	0	1	68	1	68	1.23e-22	86.3
Msa1147850	MtrunA17_Chr2g0296831	69.118	68	21	0	1	68	1	68	2.85e-22	82.8
Msa1147850	MtrunA17_Chr3g0107381	55.882	68	30	0	1	68	1	68	1.10e-21	84.0
Msa1147850	MtrunA17_Chr4g0013041	61.765	68	24	1	1	68	1	66	1.30e-21	83.2
Msa1147850	MtrunA17_Chr5g0420351	52.174	69	33	0	1	69	1	69	1.21e-20	79.7
Msa1147850	MtrunA17_Chr3g0110131	61.765	68	26	0	1	68	1	68	6.20e-20	79.7
Msa1147850	MtrunA17_Chr4g0014121	67.647	68	22	0	1	68	29	96	2.28e-19	77.0
Msa1147850	MtrunA17_Chr1g0197461	49.254	67	34	0	3	69	2	68	1.44e-18	74.3
Msa1147850	MtrunA17_Chr1g0197321	46.377	69	37	0	1	69	1	69	1.75e-18	73.9
Msa1147850	MtrunA17_Chr3g0107331	51.515	66	32	0	3	68	2	67	5.86e-18	72.8
Msa1147850	MtrunA17_Chr4g0014131	66.176	68	23	0	1	68	1	68	1.63e-17	73.2
Msa1147850	MtrunA17_Chr1g0197261	44.928	69	38	0	1	69	1	69	2.54e-17	70.9
Msa1147850	MtrunA17_Chr1g0188681	46.377	69	37	0	1	69	1	69	5.17e-17	70.1
Msa1147850	MtrunA17_Chr3g0107351	50.794	63	31	0	3	65	2	64	3.25e-16	68.2
Msa1147850	MtrunA17_Chr3g0107341	50.000	66	33	0	3	68	2	67	1.05e-15	67.0
Msa1147850	MtrunA17_Chr3g0107461	42.647	68	39	0	1	68	1	68	1.87e-15	66.2
Msa1147850	MtrunA17_Chr3g0107361	49.206	63	32	0	3	65	2	64	3.56e-15	65.5
Msa1147850	MtrunA17_Chr2g0283941	54.237	59	27	0	1	59	1	59	3.58e-15	67.0
Msa1147850	MtrunA17_Chr7g0218151	47.826	69	36	0	1	69	1	69	1.23e-14	64.3
Msa1147850	MtrunA17_Chr1g0188661	47.826	69	36	0	1	69	1	69	4.07e-14	62.8
Msa1147850	MtrunA17_Chr1g0193311	47.826	69	36	0	1	69	1	69	1.01e-13	61.6
Msa1147850	MtrunA17_Chr4g0012761	47.826	69	36	0	1	69	1	69	1.39e-13	61.6
Msa1147850	MtrunA17_Chr5g0432691	41.176	68	40	0	1	68	1	68	4.61e-13	60.1
Msa1147850	MtrunA17_Chr1g0188651	49.275	69	35	0	1	69	1	69	6.61e-13	59.7
Msa1147850	MtrunA17_Chr2g0296861	37.681	69	43	0	1	69	1	69	4.35e-12	57.4
Msa1147850	MtrunA17_Chr4g0008701	45.312	64	32	2	1	61	1	64	3.47e-11	57.0
Msa1147850	MtrunA17_Chr7g0267601	43.750	64	33	2	1	61	1	64	5.54e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1147850	AT3G05860.2	49.275	69	35	0	1	69	1	69	4.34e-19	77.0
Msa1147850	AT3G05860.3	49.275	69	35	0	1	69	1	69	1.28e-18	76.6
Msa1147850	AT3G05860.1	49.275	69	35	0	1	69	1	69	1.69e-18	76.6
Msa1147850	AT5G48670.1	60.000	65	26	0	1	65	1	65	2.36e-15	68.6
Msa1147850	AT5G27960.1	48.387	62	32	0	4	65	2	63	1.73e-13	63.5
Msa1147850	AT1G31630.1	44.118	68	37	1	3	70	2	68	2.79e-13	63.2
Msa1147850	AT5G26650.1	46.774	62	33	0	4	65	2	63	6.81e-13	62.0
Msa1147850	AT1G65300.1	43.939	66	37	0	3	68	2	67	1.67e-12	60.5
Msa1147850	AT1G65330.1	43.939	66	37	0	3	68	2	67	3.47e-12	59.7
Msa1147850	AT1G22590.2	39.706	68	41	0	1	68	1	68	8.53e-12	57.4

Find 15 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATGAACTAAGCATCCTTTA+TGG	0.192038	7_4:+27369132	Msa1147850:CDS
ACTAGCATCATTTACATTAG+AGG	0.291921	7_4:-27369061	None:intergenic
ATGAACCACAACTTGAGATT+TGG	0.331935	7_4:+27369187	Msa1147850:CDS
ATCTCAAGTTGTGGTTCATA+AGG	0.360688	7_4:-27369183	None:intergenic
CACAAGCTTCAATTCCATAA+AGG	0.419909	7_4:-27369146	None:intergenic
AGAGCACTTTGAACTCCCTT+TGG	0.428208	7_4:-27369216	None:intergenic
TCAAAGTGCTCTCGAAATCC+AGG	0.433865	7_4:+27369227	Msa1147850:CDS
TCTCAAGTTGTGGTTCATAA+GGG	0.506587	7_4:-27369182	None:intergenic
ATCCAACTCATGTATAGTCC+TGG	0.516845	7_4:-27369245	None:intergenic
CTTTGAACTCCCTTTGGCGA+TGG	0.523260	7_4:-27369210	None:intergenic
TGAGATTTGGCCATCGCCAA+AGG	0.527994	7_4:+27369200	Msa1147850:CDS
ACATACAATAAAATGAAGAA+TGG	0.549725	7_4:+27369093	Msa1147850:CDS
GAGATTTGGCCATCGCCAAA+GGG	0.554093	7_4:+27369201	Msa1147850:CDS
GAAGCTTGTGCTATAGTGTA+TGG	0.585884	7_4:+27369159	Msa1147850:CDS
GATGGCCAAATCTCAAGTTG+TGG	0.647253	7_4:-27369192	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACATACAATAAAATGAAGAA+TGG	+	chr7_4:27369093-27369112	Msa1147850:CDS	20.0%
	ACTAGCATCATTTACATTAG+AGG	-	chr7_4:27369064-27369083	None:intergenic	30.0%
	GATGAACTAAGCATCCTTTA+TGG	+	chr7_4:27369132-27369151	Msa1147850:CDS	35.0%
	CACAAGCTTCAATTCCATAA+AGG	-	chr7_4:27369149-27369168	None:intergenic	35.0%
!	TCTCAAGTTGTGGTTCATAA+GGG	-	chr7_4:27369185-27369204	None:intergenic	35.0%
!	ATCTCAAGTTGTGGTTCATA+AGG	-	chr7_4:27369186-27369205	None:intergenic	35.0%
	ATGAACCACAACTTGAGATT+TGG	+	chr7_4:27369187-27369206	Msa1147850:CDS	35.0%
	GAAGCTTGTGCTATAGTGTA+TGG	+	chr7_4:27369159-27369178	Msa1147850:CDS	40.0%
!	GATGGCCAAATCTCAAGTTG+TGG	-	chr7_4:27369195-27369214	None:intergenic	45.0%
!	AGAGCACTTTGAACTCCCTT+TGG	-	chr7_4:27369219-27369238	None:intergenic	45.0%
!	TCAAAGTGCTCTCGAAATCC+AGG	+	chr7_4:27369227-27369246	Msa1147850:CDS	45.0%
	TGAGATTTGGCCATCGCCAA+AGG	+	chr7_4:27369200-27369219	Msa1147850:CDS	50.0%
	GAGATTTGGCCATCGCCAAA+GGG	+	chr7_4:27369201-27369220	Msa1147850:CDS	50.0%
	CTTTGAACTCCCTTTGGCGA+TGG	-	chr7_4:27369213-27369232	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	27369036	27369260	27369036	ID=Msa1147850;Name=Msa1147850
chr7_4	mRNA	27369036	27369260	27369036	ID=Msa1147850-mRNA-1;Parent=Msa1147850;Name=Msa1147850-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|74
chr7_4	exon	27369036	27369260	27369036	ID=Msa1147850-mRNA-1:exon:6709;Parent=Msa1147850-mRNA-1
chr7_4	CDS	27369036	27369260	27369036	ID=Msa1147850-mRNA-1:cds;Parent=Msa1147850-mRNA-1

Gene Sequence	>Msa1147850 ATGACTAGAAAAAAGGTGAAACTTTCCTCTAATGTAAATGATGCTAGTATAAAAGCAACATACAATAAAATGAAGAATGGTGTATTAAATAAAGTTGATGAACTAAGCATCCTTTATGGAATTGAAGCTTGTGCTATAGTGTATGGCCCTTATGAACCACAACTTGAGATTTGGCCATCGCCAAAGGGAGTTCAAAGTGCTCTCGAAATCCAGGACTATACATGA
Protein sequence	>Msa1147850 MTRKKVKLSSNVNDASIKATYNKMKNGVLNKVDELSILYGIEACAIVYGPYEPQLEIWPSPKGVQSALEIQDYT*