Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1147860 | AES79095.2 | 61.053 | 95 | 8 | 2 | 1 | 72 | 13 | 101 | 1.73e-22 | 94.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1147860 | G7L066 | 61.053 | 95 | 8 | 2 | 1 | 72 | 13 | 101 | 8.28e-23 | 94.7 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1147860 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1147860 | MtrunA17_Chr8g0351751 | 70.833 | 48 | 14 | 0 | 35 | 82 | 6 | 53 | 3.70e-15 | 67.4 |
| Msa1147860 | MtrunA17_Chr4g0014321 | 60.417 | 48 | 19 | 0 | 35 | 82 | 6 | 53 | 1.15e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCACATAAGGACCTTTAAA+AGG | 0.247852 | 7_4:+27370006 | None:intergenic |
| GAAGAAGGGTCTGTTGAATA+AGG | 0.356989 | 7_4:-27369741 | Msa1147860:CDS |
| ATAGTTACTCCTCCCACATA+AGG | 0.498334 | 7_4:+27369994 | None:intergenic |
| AACATACAAGAAAACGAAGA+AGG | 0.502252 | 7_4:-27369756 | Msa1147860:CDS |
| ATCTCAGGTTATGGTTCACA+AGG | 0.529214 | 7_4:+27369665 | None:intergenic |
| TTTAAAGGTCCTTATGTGGG+AGG | 0.591138 | 7_4:-27370003 | Msa1147860:CDS |
| GGGAGGAGTAACTATGTTGG+TGG | 0.612331 | 7_4:-27369986 | Msa1147860:intron |
| ACATACAAGAAAACGAAGAA+GGG | 0.616612 | 7_4:-27369755 | Msa1147860:CDS |
| TTGGTTTATGCAGGGGAACG+AGG | 0.621187 | 7_4:-27370081 | None:intergenic |
| TGTGGGAGGAGTAACTATGT+TGG | 0.636800 | 7_4:-27369989 | Msa1147860:CDS |
| CACTAACTACAATTCTACAA+AGG | 0.644458 | 7_4:+27369702 | None:intergenic |
| AAGTTAAAGAGATTCTTGAA+CGG | 0.689895 | 7_4:-27370035 | Msa1147860:CDS |
| TCTCAGGTTATGGTTCACAA+GGG | 0.698973 | 7_4:+27369666 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGAAAACAAAATTTGAT+TGG | + | chr7_4:27369884-27369903 | None:intergenic | 15.0% |
| ! | AAGTTAAAGAGATTCTTGAA+CGG | - | chr7_4:27369707-27369726 | Msa1147860:CDS | 25.0% |
| !!! | ATATTTTTGTGCCATAAGAA+TGG | - | chr7_4:27369800-27369819 | Msa1147860:CDS | 25.0% |
| ! | GAACTTTTCATCCATTCTTA+TGG | + | chr7_4:27369814-27369833 | None:intergenic | 30.0% |
| ! | TTTTACCTTTTCTCTAGTCA+TGG | + | chr7_4:27369931-27369950 | None:intergenic | 30.0% |
| AACATACAAGAAAACGAAGA+AGG | - | chr7_4:27369986-27370005 | Msa1147860:intron | 30.0% | |
| ACATACAAGAAAACGAAGAA+GGG | - | chr7_4:27369987-27370006 | Msa1147860:CDS | 30.0% | |
| CACTAACTACAATTCTACAA+AGG | + | chr7_4:27370043-27370062 | None:intergenic | 30.0% | |
| ! | GAACGGTGTAATCCTTTTAA+AGG | - | chr7_4:27369724-27369743 | Msa1147860:CDS | 35.0% |
| !! | TCCTTTTAAAGGTCCTTATG+TGG | - | chr7_4:27369735-27369754 | Msa1147860:CDS | 35.0% |
| !! | CCTTTTAAAGGTCCTTATGT+GGG | - | chr7_4:27369736-27369755 | Msa1147860:CDS | 35.0% |
| !!! | CATGGTTTTGACAAGAACAA+AGG | + | chr7_4:27369913-27369932 | None:intergenic | 35.0% |
| CAAAACCATGACTAGAGAAA+AGG | - | chr7_4:27369923-27369942 | Msa1147860:intron | 35.0% | |
| CCCACATAAGGACCTTTAAA+AGG | + | chr7_4:27369739-27369758 | None:intergenic | 40.0% | |
| TTTAAAGGTCCTTATGTGGG+AGG | - | chr7_4:27369739-27369758 | Msa1147860:CDS | 40.0% | |
| ATAGTTACTCCTCCCACATA+AGG | + | chr7_4:27369751-27369770 | None:intergenic | 40.0% | |
| GAAGAAGGGTCTGTTGAATA+AGG | - | chr7_4:27370001-27370020 | Msa1147860:CDS | 40.0% | |
| TGTGGGAGGAGTAACTATGT+TGG | - | chr7_4:27369753-27369772 | Msa1147860:CDS | 45.0% | |
| GGGAGGAGTAACTATGTTGG+TGG | - | chr7_4:27369756-27369775 | Msa1147860:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 27369676 | 27370088 | 27369676 | ID=Msa1147860;Name=Msa1147860 |
| chr7_4 | mRNA | 27369676 | 27370088 | 27369676 | ID=Msa1147860-mRNA-1;Parent=Msa1147860;Name=Msa1147860-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|0|0|1|0|0|2|0|82 |
| chr7_4 | exon | 27369987 | 27370088 | 27369987 | ID=Msa1147860-mRNA-1:exon:6717;Parent=Msa1147860-mRNA-1 |
| chr7_4 | exon | 27369676 | 27369819 | 27369676 | ID=Msa1147860-mRNA-1:exon:6716;Parent=Msa1147860-mRNA-1 |
| chr7_4 | CDS | 27369987 | 27370088 | 27369987 | ID=Msa1147860-mRNA-1:cds;Parent=Msa1147860-mRNA-1 |
| chr7_4 | CDS | 27369676 | 27369819 | 27369676 | ID=Msa1147860-mRNA-1:cds;Parent=Msa1147860-mRNA-1 |
| Gene Sequence |
| Protein sequence |
