AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1148140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1148140 MtrunA17_Chr4g0055491 74.312 109 13 2 139 232 126 234 4.85e-46 155
Msa1148140 MtrunA17_Chr6g0476171 72.727 77 20 1 1 77 167 242 1.02e-34 124
Msa1148140 MtrunA17_Chr4g0028191 71.930 57 6 1 80 126 84 140 2.72e-16 77.4
Msa1148140 MtrunA17_Chr1g0163111 44.156 77 41 1 1 77 151 225 5.72e-15 72.0
Msa1148140 MtrunA17_Chr1g0163201 45.946 74 38 1 4 77 318 389 3.55e-14 71.2
Msa1148140 MtrunA17_Chr1g0163151 48.333 60 31 0 18 77 159 218 5.61e-14 68.9
Msa1148140 MtrunA17_Chr1g0163131 45.946 74 38 1 4 77 232 303 2.05e-13 68.6
Msa1148140 MtrunA17_Chr1g0163181 41.892 74 41 1 4 77 355 426 1.27e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1148140 AT4G17050.1 72.152 79 22 0 154 232 156 234 2.23e-33 122

Find 77 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACGTTAGATGTATGTTTC+TGG 0.212755 7_4:+28023698 None:intergenic
GAATTTACATAATAAATTAA+AGG 0.240898 7_4:-28023068 Msa1148140:five_prime_UTR
AAGGACGATCTCAGATAATA+AGG 0.259880 7_4:+28023583 None:intergenic
GAGCTTGGTGAAGGAGTAAA+TGG 0.325269 7_4:-28023388 Msa1148140:five_prime_UTR
TATGCTTAGTGTAAATTTAG+TGG 0.331972 7_4:-28022902 Msa1148140:CDS
AAAGCTACTTCCAATGTCTT+TGG 0.332406 7_4:-28021051 Msa1148140:CDS
AAGTTACAACTTCCAAGTTC+AGG 0.335044 7_4:+28022409 None:intergenic
CTTATCCAGTTGAAAGCCTT+TGG 0.336065 7_4:-28022611 Msa1148140:intron
ACTCGTGTTGAAGGAGTAAA+TGG 0.366685 7_4:-28023429 Msa1148140:five_prime_UTR
TGGAGAGTTTCTTAATGTTA+AGG 0.373459 7_4:-28020871 Msa1148140:CDS
CTGCTGTACTTCCCTGAACT+TGG 0.394540 7_4:-28022421 Msa1148140:CDS
GTCGAATTAACTAGGGGCTC+TGG 0.407908 7_4:+28021394 None:intergenic
AAGAATGAAGGTTTGGAGCT+TGG 0.431294 7_4:-28023403 Msa1148140:five_prime_UTR
TAGCTGTAGCCTCTGGTGCT+TGG 0.433968 7_4:-28021761 Msa1148140:CDS
AATTATGCATGGTTTACTAA+AGG 0.440805 7_4:-28022823 Msa1148140:CDS
GGGAAGTACAGCAGGGGAAA+GGG 0.467396 7_4:+28022430 None:intergenic
AGTTACAACTTCCAAGTTCA+GGG 0.469607 7_4:+28022410 None:intergenic
TTGCACGTCTTCCGCAGGCT+TGG 0.470773 7_4:-28020805 Msa1148140:three_prime_UTR
ATGGCAGAAGAATGAAGGTT+TGG 0.474130 7_4:-28023410 Msa1148140:five_prime_UTR
ATGCATGGTTTACTAAAGGT+TGG 0.475180 7_4:-28022819 Msa1148140:CDS
ACGGTTTGAACTCGTGTTGA+AGG 0.478800 7_4:-28023438 Msa1148140:five_prime_UTR
AGCAGCGATTATGCTGTGTC+TGG 0.479389 7_4:-28021917 Msa1148140:CDS
CAAGTTCAGGGAAGTACAGC+AGG 0.482827 7_4:+28022422 None:intergenic
TCGAATTAACTAGGGGCTCT+GGG 0.484257 7_4:+28021395 None:intergenic
AGGACGATCTCAGATAATAA+GGG 0.488352 7_4:+28023584 None:intergenic
GTACCTCCCCTGGAGTTTCA+AGG 0.489536 7_4:+28021356 None:intergenic
AATTTCATCTTCTTCTCCAA+AGG 0.492981 7_4:+28022595 None:intergenic
GTCTATCCGTCGAATTAACT+AGG 0.494132 7_4:+28021386 None:intergenic
AGGGAAGTACAGCAGGGGAA+AGG 0.494848 7_4:+28022429 None:intergenic
TGAAAGTAGAGGAAGGATCA+TGG 0.496049 7_4:-28022876 Msa1148140:CDS
TAGGAATAATGTACCTCCCC+TGG 0.496765 7_4:+28021346 None:intergenic
AGTAAATGGCAGAAGAATGA+AGG 0.500272 7_4:-28023415 Msa1148140:five_prime_UTR
CAGCCACTCCTTGAAACTCC+AGG 0.501354 7_4:-28021364 Msa1148140:CDS
AGGGAATGCAAAGTGAAGAT+TGG 0.504671 7_4:-28022784 Msa1148140:CDS
ACTAAATTCTGTGGTGGAGT+TGG 0.509338 7_4:-28022362 Msa1148140:CDS
AGACACAGCATAATCGCTGC+TGG 0.515058 7_4:+28021919 None:intergenic
GAAGGTTTGGAGCTTGGTGA+AGG 0.518665 7_4:-28023397 Msa1148140:five_prime_UTR
AGCACCAGAGGCTACAGCTA+TGG 0.521588 7_4:+28021764 None:intergenic
GCGATTATGCTGTGTCTGGA+GGG 0.522335 7_4:-28021913 Msa1148140:CDS
TCAAATACCACAATAATAGC+AGG 0.532548 7_4:+28021708 None:intergenic
AGAGCCATAGCTGTAGCCTC+TGG 0.535902 7_4:-28021768 Msa1148140:CDS
TTTAGTGGAGTTGAAAGTAG+AGG 0.536584 7_4:-28022887 Msa1148140:CDS
GTGGAGTTGAAAGTAGAGGA+AGG 0.538949 7_4:-28022883 Msa1148140:CDS
TCCCCTGGAGTTTCAAGGAG+TGG 0.539979 7_4:+28021361 None:intergenic
TCTATCCGTCGAATTAACTA+GGG 0.541970 7_4:+28021387 None:intergenic
AGCGATTATGCTGTGTCTGG+AGG 0.545559 7_4:-28021914 Msa1148140:CDS
ATGGCTCGTGTTCATTCTTG+AGG 0.545951 7_4:-28023369 Msa1148140:five_prime_UTR
TTCAAAATGCCAAGCACCAG+AGG 0.549393 7_4:+28021752 None:intergenic
GGAAGGATCATGGTTTGTAG+AGG 0.555796 7_4:-28022866 Msa1148140:CDS
ATCAACATCTCAATTATGCA+TGG 0.560159 7_4:-28022834 Msa1148140:CDS
AAGTCGTATGCCAAAGACAT+TGG 0.570777 7_4:+28021041 None:intergenic
CGTTTACATGACAAAGAAGA+CGG 0.571444 7_4:-28023457 Msa1148140:five_prime_UTR
TGAAGGGAAAACGATTGAGA+AGG 0.571929 7_4:+28023560 None:intergenic
CGATTATGCTGTGTCTGGAG+GGG 0.573490 7_4:-28021912 Msa1148140:intron
CGACGCTCCTGCTATTATTG+TGG 0.578943 7_4:-28021715 Msa1148140:CDS
AGCCACTCCTTGAAACTCCA+GGG 0.583132 7_4:-28021363 Msa1148140:CDS
CACAATAATAGCAGGAGCGT+CGG 0.583690 7_4:+28021716 None:intergenic
AAGGTTGGCAAGAATTTGTG+AGG 0.583765 7_4:-28022804 Msa1148140:CDS
TTATTGTGGTATTTGAACGA+AGG 0.594533 7_4:-28021701 Msa1148140:intron
TTAACATTAAGAAACTCTCC+AGG 0.597029 7_4:+28020873 None:intergenic
TGTCTCTTGAACAACTCACT+TGG 0.603863 7_4:+28022928 None:intergenic
GGGAAAACGATTGAGAAGGA+AGG 0.609715 7_4:+28023564 None:intergenic
GAACAACAGACTAAATTCTG+TGG 0.616625 7_4:-28022371 Msa1148140:CDS
TCATTCTTGAGGATCTGTTG+AGG 0.618909 7_4:-28023358 Msa1148140:intron
TTTCGTAACATCATTAGACA+TGG 0.633055 7_4:-28023044 Msa1148140:intron
TTTAACAGAAAACGAGAGAG+AGG 0.634959 7_4:+28023497 None:intergenic
GTGGAGTTGGCTGCAGACAA+AGG 0.637148 7_4:-28022349 Msa1148140:intron
CAACAGACTAAATTCTGTGG+TGG 0.639371 7_4:-28022368 Msa1148140:CDS
AGAGCCCCTAGTTAATTCGA+CGG 0.642003 7_4:-28021392 Msa1148140:CDS
TTTATTAGATACCAAGCCTG+CGG 0.649575 7_4:+28020794 None:intergenic
GAAAATGAGATGAAGAACAA+CGG 0.650333 7_4:+28023606 None:intergenic
AAGTTCAGGGAAGTACAGCA+GGG 0.655737 7_4:+28022423 None:intergenic
AGGTTGGCAAGAATTTGTGA+GGG 0.666130 7_4:-28022803 Msa1148140:CDS
GCCACTCCTTGAAACTCCAG+GGG 0.670430 7_4:-28021362 Msa1148140:CDS
ACTCCTTGAAACTCCAGGGG+AGG 0.676831 7_4:-28021359 Msa1148140:intron
CTATCCGTCGAATTAACTAG+GGG 0.696026 7_4:+28021388 None:intergenic
AGTTCAGGGAAGTACAGCAG+GGG 0.708443 7_4:+28022424 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAATTTACATAATAAATTAA+AGG - chr7_4:28021300-28021319 Msa1148140:intron 10.0%
!! TTAAAAACAAATGTAATAAC+AGG + chr7_4:28021684-28021703 None:intergenic 15.0%
!!! TAATTTTATTGAAAATGCAT+TGG - chr7_4:28022782-28022801 Msa1148140:CDS 15.0%
!! TTGTTGAATTTAAACAAAAA+AGG + chr7_4:28023077-28023096 None:intergenic 15.0%
!! ATAATATTAGAAAGATTAGA+CGG + chr7_4:28023409-28023428 None:intergenic 15.0%
!! TAATCTTTCTAATATTATCA+TGG - chr7_4:28023411-28023430 Msa1148140:five_prime_UTR 15.0%
!!! AGTTTATTTTGTTAGTTTTT+TGG - chr7_4:28023618-28023637 Msa1148140:five_prime_UTR 15.0%
!! TAACAACACAAAAACAAAAA+GGG + chr7_4:28021129-28021148 None:intergenic 20.0%
!! CACTAAATTAAGACATATTA+AGG - chr7_4:28022092-28022111 Msa1148140:intron 20.0%
!!! AATTATTGATTTGTGTAACT+AGG + chr7_4:28022178-28022197 None:intergenic 20.0%
!!! TAAATTCCATTCTTTTATCT+TGG - chr7_4:28022502-28022521 Msa1148140:intron 20.0%
!! GTTTATAAATAATAAAAGGG+TGG + chr7_4:28022709-28022728 None:intergenic 20.0%
!! TTTGTTGCATTAATTCAAAT+TGG - chr7_4:28022891-28022910 Msa1148140:CDS 20.0%
!! GATAGTTAATCGAAAAATTA+AGG - chr7_4:28023119-28023138 Msa1148140:intron 20.0%
!! CTTAAGTATATGAGAAATTT+GGG + chr7_4:28023368-28023387 None:intergenic 20.0%
!! TTCTAATATTATCATGGTTT+CGG - chr7_4:28023417-28023436 Msa1148140:five_prime_UTR 20.0%
! TAACAAAAAAACGAGATGAA+GGG + chr7_4:28020827-28020846 None:intergenic 25.0%
! TTAACAAAAAAACGAGATGA+AGG + chr7_4:28020828-28020847 None:intergenic 25.0%
! CAAAAAGGGAAAATCAATTA+GGG + chr7_4:28021115-28021134 None:intergenic 25.0%
! ACAAAAAGGGAAAATCAATT+AGG + chr7_4:28021116-28021135 None:intergenic 25.0%
! CTAACAACACAAAAACAAAA+AGG + chr7_4:28021130-28021149 None:intergenic 25.0%
! TATGCTTAGTGTAAATTTAG+TGG - chr7_4:28021466-28021485 Msa1148140:intron 25.0%
! AATTATGCATGGTTTACTAA+AGG - chr7_4:28021545-28021564 Msa1148140:intron 25.0%
! TCTAAGTAGTTCTCAAAATA+TGG - chr7_4:28021848-28021867 Msa1148140:intron 25.0%
!!! TGTTTAGTTTTCTGCATAAA+TGG - chr7_4:28022205-28022224 Msa1148140:intron 25.0%
! CAAAAACAGAATGAATGAAA+TGG + chr7_4:28022267-28022286 None:intergenic 25.0%
! TATAAATAATAAAAGGGTGG+AGG + chr7_4:28022706-28022725 None:intergenic 25.0%
! AGAATCTTCAAATGTGAAAT+GGG + chr7_4:28022812-28022831 None:intergenic 25.0%
! AAGAATCTTCAAATGTGAAA+TGG + chr7_4:28022813-28022832 None:intergenic 25.0%
!! ACCATTAACATTGATAATAG+TGG + chr7_4:28023145-28023164 None:intergenic 25.0%
! AAGCAATTCTTAAATGCTTT+GGG - chr7_4:28023173-28023192 Msa1148140:intron 25.0%
! CCTTAAGTATATGAGAAATT+TGG + chr7_4:28023369-28023388 None:intergenic 25.0%
! CCAAATTTCTCATATACTTA+AGG - chr7_4:28023366-28023385 Msa1148140:five_prime_UTR 25.0%
GAAAATGAGATGAAGAACAA+CGG + chr7_4:28020765-28020784 None:intergenic 30.0%
TTATTGTGCACAACTAATCA+AGG - chr7_4:28021230-28021249 Msa1148140:intron 30.0%
TTTCGTAACATCATTAGACA+TGG - chr7_4:28021324-28021343 Msa1148140:intron 30.0%
TTTGTTCTTATATATGCAGC+TGG - chr7_4:28021412-28021431 Msa1148140:CDS 30.0%
ATCAACATCTCAATTATGCA+TGG - chr7_4:28021534-28021553 Msa1148140:intron 30.0%
AATTTCATCTTCTTCTCCAA+AGG + chr7_4:28021776-28021795 None:intergenic 30.0%
AAGAAGATGAAATTCCAGTT+TGG - chr7_4:28021782-28021801 Msa1148140:intron 30.0%
AGAAGATGAAATTCCAGTTT+GGG - chr7_4:28021783-28021802 Msa1148140:intron 30.0%
! TTTTCTGCATAAATGGAAGT+TGG - chr7_4:28022212-28022231 Msa1148140:intron 30.0%
ACTTAGCAATGACTTTATTC+CGG - chr7_4:28022291-28022310 Msa1148140:intron 30.0%
CAAAAGCCAAGATAAAAGAA+TGG + chr7_4:28022511-28022530 None:intergenic 30.0%
!! TCTTTTATCTTGGCTTTTGT+TGG - chr7_4:28022512-28022531 Msa1148140:intron 30.0%
TCAAATACCACAATAATAGC+AGG + chr7_4:28022663-28022682 None:intergenic 30.0%
TTATTGTGGTATTTGAACGA+AGG - chr7_4:28022667-28022686 Msa1148140:intron 30.0%
TTTCATTCTGATGAATGATC+AGG + chr7_4:28022751-28022770 None:intergenic 30.0%
AGTAATGTAAATAACCTGTC+AGG - chr7_4:28022927-28022946 Msa1148140:CDS 30.0%
GGTACATTATTCCTATATTC+TGG - chr7_4:28023030-28023049 Msa1148140:intron 30.0%
CATTATTCCTATATTCTGGA+AGG - chr7_4:28023034-28023053 Msa1148140:intron 30.0%
GCCACTATTATCAATGTTAA+TGG - chr7_4:28023141-28023160 Msa1148140:intron 30.0%
GAAGCAATTCTTAAATGCTT+TGG - chr7_4:28023172-28023191 Msa1148140:intron 30.0%
TTAACATTAAGAAACTCTCC+AGG + chr7_4:28023498-28023517 None:intergenic 30.0%
TGGAGAGTTTCTTAATGTTA+AGG - chr7_4:28023497-28023516 Msa1148140:five_prime_UTR 30.0%
AGGACGATCTCAGATAATAA+GGG + chr7_4:28020787-28020806 None:intergenic 35.0%
AAGGACGATCTCAGATAATA+AGG + chr7_4:28020788-28020807 None:intergenic 35.0%
TTTAACAGAAAACGAGAGAG+AGG + chr7_4:28020874-28020893 None:intergenic 35.0%
CGTTTACATGACAAAGAAGA+CGG - chr7_4:28020911-28020930 Msa1148140:intron 35.0%
AGTAAATGGCAGAAGAATGA+AGG - chr7_4:28020953-28020972 Msa1148140:intron 35.0%
CAATACACACATCAGTATCA+GGG + chr7_4:28021178-28021197 None:intergenic 35.0%
TTTAGTGGAGTTGAAAGTAG+AGG - chr7_4:28021481-28021500 Msa1148140:intron 35.0%
ATGCATGGTTTACTAAAGGT+TGG - chr7_4:28021549-28021568 Msa1148140:intron 35.0%
GAAGATGAAATTCCAGTTTG+GGG - chr7_4:28021784-28021803 Msa1148140:intron 35.0%
AAGATGAAATTCCAGTTTGG+GGG - chr7_4:28021785-28021804 Msa1148140:intron 35.0%
!!! GGTAGAAGTTTTTGGTGAAA+AGG - chr7_4:28021806-28021825 Msa1148140:intron 35.0%
AGTTACAACTTCCAAGTTCA+GGG + chr7_4:28021961-28021980 None:intergenic 35.0%
AAGTTACAACTTCCAAGTTC+AGG + chr7_4:28021962-28021981 None:intergenic 35.0%
GAACAACAGACTAAATTCTG+TGG - chr7_4:28021997-28022016 Msa1148140:intron 35.0%
GGAATAAAGTCATTGCTAAG+TGG + chr7_4:28022292-28022311 None:intergenic 35.0%
CACTCATAAAACATACAAGC+CGG + chr7_4:28022313-28022332 None:intergenic 35.0%
! GTATGTTTTATGAGTGGAGT+TGG - chr7_4:28022317-28022336 Msa1148140:intron 35.0%
GTGTTTAGAGACTTAGCTAT+GGG - chr7_4:28022547-28022566 Msa1148140:intron 35.0%
! TTAGCTATGGGACTGAATTT+TGG - chr7_4:28022559-28022578 Msa1148140:intron 35.0%
TGATGAATGATCAGGTCATA+GGG + chr7_4:28022743-28022762 None:intergenic 35.0%
TCTATCCGTCGAATTAACTA+GGG + chr7_4:28022984-28023003 None:intergenic 35.0%
CATAATGCCTTCCAGAATAT+AGG + chr7_4:28023044-28023063 None:intergenic 35.0%
! TTCTTAAATGCTTTGGGTTC+AGG - chr7_4:28023179-28023198 Msa1148140:intron 35.0%
AAAGCTACTTCCAATGTCTT+TGG - chr7_4:28023317-28023336 Msa1148140:intron 35.0%
AAATCCATTATCTGCAAGCA+TGG + chr7_4:28023471-28023490 None:intergenic 35.0%
TTTATTAGATACCAAGCCTG+CGG + chr7_4:28023577-28023596 None:intergenic 35.0%
!!! GAGATGTGTTTTGAGAAGGA+AGG + chr7_4:28020743-28020762 None:intergenic 40.0%
!!! AACGGAGATGTGTTTTGAGA+AGG + chr7_4:28020747-28020766 None:intergenic 40.0%
TGAAGGGAAAACGATTGAGA+AGG + chr7_4:28020811-28020830 None:intergenic 40.0%
ACTCGTGTTGAAGGAGTAAA+TGG - chr7_4:28020939-28020958 Msa1148140:intron 40.0%
ATGGCAGAAGAATGAAGGTT+TGG - chr7_4:28020958-28020977 Msa1148140:intron 40.0%
! AAGAATGAAGGTTTGGAGCT+TGG - chr7_4:28020965-28020984 Msa1148140:intron 40.0%
TCATTCTTGAGGATCTGTTG+AGG - chr7_4:28021010-28021029 Msa1148140:intron 40.0%
CAGTATCAGGGTCTCAATTA+GGG + chr7_4:28021166-28021185 None:intergenic 40.0%
TCAGTATCAGGGTCTCAATT+AGG + chr7_4:28021167-28021186 None:intergenic 40.0%
GCAATACACACATCAGTATC+AGG + chr7_4:28021179-28021198 None:intergenic 40.0%
TGATGTGTGTATTGCTCAAG+TGG - chr7_4:28021184-28021203 Msa1148140:intron 40.0%
TGTCTCTTGAACAACTCACT+TGG + chr7_4:28021443-28021462 None:intergenic 40.0%
TGAAAGTAGAGGAAGGATCA+TGG - chr7_4:28021492-28021511 Msa1148140:intron 40.0%
AAGGTTGGCAAGAATTTGTG+AGG - chr7_4:28021564-28021583 Msa1148140:intron 40.0%
AGGTTGGCAAGAATTTGTGA+GGG - chr7_4:28021565-28021584 Msa1148140:intron 40.0%
AGGGAATGCAAAGTGAAGAT+TGG - chr7_4:28021584-28021603 Msa1148140:intron 40.0%
CTTATCCAGTTGAAAGCCTT+TGG - chr7_4:28021757-28021776 Msa1148140:CDS 40.0%
AAAAACTTCTACCCCCAAAC+TGG + chr7_4:28021799-28021818 None:intergenic 40.0%
! AGTTTGGGGGTAGAAGTTTT+TGG - chr7_4:28021798-28021817 Msa1148140:intron 40.0%
CAACAGACTAAATTCTGTGG+TGG - chr7_4:28022000-28022019 Msa1148140:intron 40.0%
! ACTAAATTCTGTGGTGGAGT+TGG - chr7_4:28022006-28022025 Msa1148140:intron 40.0%
TGTGTAACTAGGCACTTCTA+TGG + chr7_4:28022167-28022186 None:intergenic 40.0%
! CGGCTTGTATGTTTTATGAG+TGG - chr7_4:28022311-28022330 Msa1148140:intron 40.0%
!!! AGGGGTCTTAGCAGTTTTTT+TGG - chr7_4:28022474-28022493 Msa1148140:intron 40.0%
!!! GGGGTCTTAGCAGTTTTTTT+GGG - chr7_4:28022475-28022494 Msa1148140:intron 40.0%
!!! GGGTCTTAGCAGTTTTTTTG+GGG - chr7_4:28022476-28022495 Msa1148140:intron 40.0%
!!! GGTCTTAGCAGTTTTTTTGG+GGG - chr7_4:28022477-28022496 Msa1148140:intron 40.0%
!!! TCTTGGCTTTTGTTGGTCTT+TGG - chr7_4:28022519-28022538 Msa1148140:intron 40.0%
GGTGTTTAGAGACTTAGCTA+TGG - chr7_4:28022546-28022565 Msa1148140:intron 40.0%
CTGATGAATGATCAGGTCAT+AGG + chr7_4:28022744-28022763 None:intergenic 40.0%
CTATCCGTCGAATTAACTAG+GGG + chr7_4:28022983-28023002 None:intergenic 40.0%
GTCTATCCGTCGAATTAACT+AGG + chr7_4:28022985-28023004 None:intergenic 40.0%
AAGTCGTATGCCAAAGACAT+TGG + chr7_4:28023330-28023349 None:intergenic 40.0%
! TAGACGGACTTTGACAAAGT+AGG + chr7_4:28023393-28023412 None:intergenic 40.0%
TGTACCATGCTTGCAGATAA+TGG - chr7_4:28023464-28023483 Msa1148140:five_prime_UTR 40.0%
!!! CAGATAATGGATTTTCAGCC+TGG - chr7_4:28023477-28023496 Msa1148140:five_prime_UTR 40.0%
GGGAAAACGATTGAGAAGGA+AGG + chr7_4:28020807-28020826 None:intergenic 45.0%
ACGGTTTGAACTCGTGTTGA+AGG - chr7_4:28020930-28020949 Msa1148140:intron 45.0%
! GAGCTTGGTGAAGGAGTAAA+TGG - chr7_4:28020980-28020999 Msa1148140:intron 45.0%
ATGGCTCGTGTTCATTCTTG+AGG - chr7_4:28020999-28021018 Msa1148140:intron 45.0%
GTGGAGTTGAAAGTAGAGGA+AGG - chr7_4:28021485-28021504 Msa1148140:intron 45.0%
! GGAAGGATCATGGTTTGTAG+AGG - chr7_4:28021502-28021521 Msa1148140:intron 45.0%
CTTCTCCAAAGGCTTTCAAC+TGG + chr7_4:28021765-28021784 None:intergenic 45.0%
! AAGTTCAGGGAAGTACAGCA+GGG + chr7_4:28021948-28021967 None:intergenic 45.0%
ATGAGTGGAGTTGGAGTCAA+CGG - chr7_4:28022326-28022345 Msa1148140:intron 45.0%
TTGATACGATAGTGTGCAGC+AGG - chr7_4:28022355-28022374 Msa1148140:CDS 45.0%
!! TACTGCTATGTTGGTTCTGC+TGG - chr7_4:28022397-28022416 Msa1148140:CDS 45.0%
!!! GCTTTTGTTGGTCTTTGGCT+GGG - chr7_4:28022524-28022543 Msa1148140:intron 45.0%
!!! CTTTTGTTGGTCTTTGGCTG+GGG - chr7_4:28022525-28022544 Msa1148140:intron 45.0%
!!! TGGGACTGAATTTTGGTGCT+TGG - chr7_4:28022566-28022585 Msa1148140:intron 45.0%
TTCAAAATGCCAAGCACCAG+AGG + chr7_4:28022619-28022638 None:intergenic 45.0%
! CACAATAATAGCAGGAGCGT+CGG + chr7_4:28022655-28022674 None:intergenic 45.0%
TGCACTTTGTACAGCGTTCA+AGG - chr7_4:28022857-28022876 Msa1148140:CDS 45.0%
AGCAGATAAGCATACCTGAC+AGG + chr7_4:28022944-28022963 None:intergenic 45.0%
TCGAATTAACTAGGGGCTCT+GGG + chr7_4:28022976-28022995 None:intergenic 45.0%
AGAGCCCCTAGTTAATTCGA+CGG - chr7_4:28022976-28022995 Msa1148140:intron 45.0%
TAGGAATAATGTACCTCCCC+TGG + chr7_4:28023025-28023044 None:intergenic 45.0%
!! TTTGTTTATAAATAATAAAA+GGG + chr7_4:28022712-28022731 None:intergenic 5.0%
!! ATTTGTTTATAAATAATAAA+AGG + chr7_4:28022713-28022732 None:intergenic 5.0%
! GAAGGTTTGGAGCTTGGTGA+AGG - chr7_4:28020971-28020990 Msa1148140:intron 50.0%
! AGTTCAGGGAAGTACAGCAG+GGG + chr7_4:28021947-28021966 None:intergenic 50.0%
! CAAGTTCAGGGAAGTACAGC+AGG + chr7_4:28021949-28021968 None:intergenic 50.0%
CTGCTGTACTTCCCTGAACT+TGG - chr7_4:28021947-28021966 Msa1148140:CDS 50.0%
GTCAGTGGCTACTGCTATGT+TGG - chr7_4:28022388-28022407 Msa1148140:CDS 50.0%
AGACACAGCATAATCGCTGC+TGG + chr7_4:28022452-28022471 None:intergenic 50.0%
AGCAGCGATTATGCTGTGTC+TGG - chr7_4:28022451-28022470 Msa1148140:intron 50.0%
AGCGATTATGCTGTGTCTGG+AGG - chr7_4:28022454-28022473 Msa1148140:intron 50.0%
GCGATTATGCTGTGTCTGGA+GGG - chr7_4:28022455-28022474 Msa1148140:intron 50.0%
CGATTATGCTGTGTCTGGAG+GGG - chr7_4:28022456-28022475 Msa1148140:intron 50.0%
!!! GGCTTTTGTTGGTCTTTGGC+TGG - chr7_4:28022523-28022542 Msa1148140:intron 50.0%
CGACGCTCCTGCTATTATTG+TGG - chr7_4:28022653-28022672 Msa1148140:intron 50.0%
GTCGAATTAACTAGGGGCTC+TGG + chr7_4:28022977-28022996 None:intergenic 50.0%
AGCCACTCCTTGAAACTCCA+GGG - chr7_4:28023005-28023024 Msa1148140:intron 50.0%
!!! GTTTTTTGCACGTCTTCCGC+AGG - chr7_4:28023558-28023577 Msa1148140:five_prime_UTR 50.0%
! GGGAAGTACAGCAGGGGAAA+GGG + chr7_4:28021941-28021960 None:intergenic 55.0%
! AGGGAAGTACAGCAGGGGAA+AGG + chr7_4:28021942-28021961 None:intergenic 55.0%
GTGGAGTTGGCTGCAGACAA+AGG - chr7_4:28022019-28022038 Msa1148140:intron 55.0%
GGCTGCAGACAAAGGTTGCT+AGG - chr7_4:28022027-28022046 Msa1148140:intron 55.0%
GCAGGACTGTGATGAGTCAG+TGG - chr7_4:28022373-28022392 Msa1148140:CDS 55.0%
AGAGCCATAGCTGTAGCCTC+TGG - chr7_4:28022600-28022619 Msa1148140:CDS 55.0%
AGCACCAGAGGCTACAGCTA+TGG + chr7_4:28022607-28022626 None:intergenic 55.0%
TAGCTGTAGCCTCTGGTGCT+TGG - chr7_4:28022607-28022626 Msa1148140:intron 55.0%
CAGCCACTCCTTGAAACTCC+AGG - chr7_4:28023004-28023023 Msa1148140:intron 55.0%
GCCACTCCTTGAAACTCCAG+GGG - chr7_4:28023006-28023025 Msa1148140:intron 55.0%
TCCCCTGGAGTTTCAAGGAG+TGG + chr7_4:28023010-28023029 None:intergenic 55.0%
ACTCCTTGAAACTCCAGGGG+AGG - chr7_4:28023009-28023028 Msa1148140:intron 55.0%
GTACCTCCCCTGGAGTTTCA+AGG + chr7_4:28023015-28023034 None:intergenic 55.0%
TTGCACGTCTTCCGCAGGCT+TGG - chr7_4:28023563-28023582 Msa1148140:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 28020671 28023719 28020671 ID=Msa1148140;Name=Msa1148140
chr7_4 mRNA 28020671 28023719 28020671 ID=Msa1148140-mRNA-1;Parent=Msa1148140;Name=Msa1148140-mRNA-1;_AED=0.20;_eAED=0.20;_QI=417|0.55|0.7|1|0.88|0.8|10|162|244
chr7_4 exon 28020671 28020910 28020671 ID=Msa1148140-mRNA-1:exon:6869;Parent=Msa1148140-mRNA-1
chr7_4 exon 28021031 28021087 28021031 ID=Msa1148140-mRNA-1:exon:6868;Parent=Msa1148140-mRNA-1
chr7_4 exon 28021360 28021441 28021360 ID=Msa1148140-mRNA-1:exon:6867;Parent=Msa1148140-mRNA-1
chr7_4 exon 28021702 28021788 28021702 ID=Msa1148140-mRNA-1:exon:6866;Parent=Msa1148140-mRNA-1
chr7_4 exon 28021913 28021967 28021913 ID=Msa1148140-mRNA-1:exon:6865;Parent=Msa1148140-mRNA-1
chr7_4 exon 28022350 28022455 28022350 ID=Msa1148140-mRNA-1:exon:6864;Parent=Msa1148140-mRNA-1
chr7_4 exon 28022592 28022624 28022592 ID=Msa1148140-mRNA-1:exon:6863;Parent=Msa1148140-mRNA-1
chr7_4 exon 28022726 28022959 28022726 ID=Msa1148140-mRNA-1:exon:6862;Parent=Msa1148140-mRNA-1
chr7_4 exon 28023045 28023103 28023045 ID=Msa1148140-mRNA-1:exon:6861;Parent=Msa1148140-mRNA-1
chr7_4 exon 28023359 28023719 28023359 ID=Msa1148140-mRNA-1:exon:6860;Parent=Msa1148140-mRNA-1
chr7_4 five_prime_UTR 28023359 28023719 28023359 ID=Msa1148140-mRNA-1:five_prime_utr;Parent=Msa1148140-mRNA-1
chr7_4 five_prime_UTR 28023048 28023103 28023048 ID=Msa1148140-mRNA-1:five_prime_utr;Parent=Msa1148140-mRNA-1
chr7_4 CDS 28023045 28023047 28023045 ID=Msa1148140-mRNA-1:cds;Parent=Msa1148140-mRNA-1
chr7_4 CDS 28022726 28022959 28022726 ID=Msa1148140-mRNA-1:cds;Parent=Msa1148140-mRNA-1
chr7_4 CDS 28022592 28022624 28022592 ID=Msa1148140-mRNA-1:cds;Parent=Msa1148140-mRNA-1
chr7_4 CDS 28022350 28022455 28022350 ID=Msa1148140-mRNA-1:cds;Parent=Msa1148140-mRNA-1
chr7_4 CDS 28021913 28021967 28021913 ID=Msa1148140-mRNA-1:cds;Parent=Msa1148140-mRNA-1
chr7_4 CDS 28021702 28021788 28021702 ID=Msa1148140-mRNA-1:cds;Parent=Msa1148140-mRNA-1
chr7_4 CDS 28021360 28021441 28021360 ID=Msa1148140-mRNA-1:cds;Parent=Msa1148140-mRNA-1
chr7_4 CDS 28021031 28021087 28021031 ID=Msa1148140-mRNA-1:cds;Parent=Msa1148140-mRNA-1
chr7_4 CDS 28020833 28020910 28020833 ID=Msa1148140-mRNA-1:cds;Parent=Msa1148140-mRNA-1
chr7_4 three_prime_UTR 28020671 28020832 28020671 ID=Msa1148140-mRNA-1:three_prime_utr;Parent=Msa1148140-mRNA-1
Gene Sequence

>Msa1148140

ATGCTGGTTGTACCAAGTGAGTTGTTCAAGAGACATAATATGCTTAGTGTAAATTTAGTGGAGTTGAAAGTAGAGGAAGGATCATGGTTTGTAGAGGTGAGTTATAATCAACATCTCAATTATGCATGGTTTACTAAAGGTTGGCAAGAATTTGTGAGGGAATGCAAAGTGAAGATTGGAGAAACTTGTTTGTTTGAGATTATTGATGTGCAAAATCATGTGTTCAAGATTATTGATTTGAAAGCCTTTGGAGAAGAAGATGAAATTCCAAAGCCCTTTCCCCTGCTGTACTTCCCTGAACTTGGAAGTTGTAACTTTATACAATCTTCTTTGAACAACAGACTAAATTCTGTGGTGGAGTTGGCTGCAGACAAAGCGCTTCTAAGCTGCTGTCTTGACAGTCCAGCAGCGATTATGCTGTGTCTGGAGGGAGCCATAGCTGTAGCCTCTGGTGCTTGGCATTTTGAACATTCAATTGAATCCGACGCTCCTGCTATTATTGTGGTATTTGAACGAAGGTATGCTTATCTGCTAAATCATGTCCCAGAGCCCCTAGTTAATTCGACGGATAGACAGCCACTCCTTGAAACTCCAGGGGAGATTTTTGAACTGCGAAAGCTACTTCCAATGTCTTTGGCATACGACTTCAATATACATATAATGGATTTTCAGCCTGGAGAGTTTCTTAATGTTAAGGTATTCATATATGAGTCTACGAGACAGTTAGTTTGTTAA
Protein sequence

>Msa1148140

MLVVPSELFKRHNMLSVNLVELKVEEGSWFVEVSYNQHLNYAWFTKGWQEFVRECKVKIGETCLFEIIDVQNHVFKIIDLKAFGEEDEIPKPFPLLYFPELGSCNFIQSSLNNRLNSVVELAADKALLSCCLDSPAAIMLCLEGAIAVASGAWHFEHSIESDAPAIIVVFERRYAYLLNHVPEPLVNSTDRQPLLETPGEIFELRKLLPMSLAYDFNIHIMDFQPGEFLNVKVFIYESTRQLVC*