AlfalfaGEDB | Msa1148400

M. sativa cultivar zhongmu-4 / Msa1148400

KEGG: K04733
GO: -
PFAM: PF00954.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1148400	KEH22734.1	86.691	556	49	2	1	531	204	759	0.0	983

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1148400	sp\|Q9SXB8\|Y1133_ARATH	50.178	562	236	3	14	531	247	808	0.0	570

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1148400	A0A072TZ74	86.306	555	47	3	1	531	252	801	0.0	985

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1148400	MtrunA17_Chr7g0236551	86.306	555	47	3	1	531	224	773	984
Msa1148400	MtrunA17_Chr7g0236511	80.610	557	61	2	1	531	230	765	914
Msa1148400	MtrunA17_Chr7g0236471	74.153	561	103	5	5	531	73	625	833
Msa1148400	MtrunA17_Chr7g0236341	68.718	585	123	5	1	531	224	802	814
Msa1148400	MtrunA17_Chr7g0236561	69.676	587	121	4	1	531	204	789	806
Msa1148400	MtrunA17_Chr7g0236651	71.949	549	136	3	1	531	222	770	803
Msa1148400	MtrunA17_Chr7g0236691	70.819	562	133	4	1	531	224	785	796
Msa1148400	MtrunA17_Chr7g0236571	70.310	549	141	4	1	531	76	620	779
Msa1148400	MtrunA17_Chr7g0236641	65.077	587	149	5	1	531	224	810	756
Msa1148400	MtrunA17_Chr7g0236121	67.021	564	152	8	1	531	226	788	748

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1148400	AT1G11330.2	50.178	562	236	3	14	531	247	808	0.0	570
Msa1148400	AT1G11330.1	50.446	561	234	4	14	531	247	806	0.0	570
Msa1148400	AT1G11300.2	51.391	539	231	6	14	531	243	771	0.0	554
Msa1148400	AT1G11300.1	49.458	554	234	5	14	531	243	786	0.0	549
Msa1148400	AT1G11303.1	48.917	554	237	6	14	531	243	786	0.0	542
Msa1148400	AT1G11350.1	50.368	544	227	6	31	531	253	796	0.0	529
Msa1148400	AT1G11330.3	51.515	495	196	3	14	464	247	741	8.90e-175	511
Msa1148400	AT1G61370.2	51.683	505	204	9	63	531	133	633	5.36e-173	504
Msa1148400	AT1G61370.1	51.683	505	204	9	63	531	284	784	3.08e-171	504
Msa1148400	AT4G21390.1	46.690	574	238	9	16	531	249	812	4.41e-163	484

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Find 99 sgRNAs with CRISPR-Local

Find 303 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAACTCCTAGATTGG+AGG	0.547566	7_4:-28528645	Msa1148400:CDS
AAAAGACTTTCTAGAGCATC+TGG	0.413825	7_4:-28529005	Msa1148400:CDS
AAAATAGTAAGTTACCTGGG+TGG	0.726008	7_4:+28529361	None:intergenic
AAACATCACATTCTGCGTTT+TGG	0.324742	7_4:+28530127	None:intergenic
AAACTTGGACAGGGTGGTTT+TGG	0.280733	7_4:-28529149	Msa1148400:CDS
AACCCAGAAATACTCGTGAA+TGG	0.494122	7_4:-28530032	Msa1148400:CDS
AACGCAGAATGTGATGTTTA+TGG	0.264759	7_4:-28530123	Msa1148400:CDS
AACTCCTAGATTGGAGGACA+CGG	0.634373	7_4:-28528639	Msa1148400:CDS
AAGATCAGGCCAATACTAGA+AGG	0.493595	7_4:-28528453	Msa1148400:CDS
AAGGAATATTTGAAGAAACA+TGG	0.647118	7_4:-28530191	Msa1148400:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATATATCACCTGAATTCACA+AGG	-	chr7_4:28527997-28528016	Msa1148400:CDS	30.0%
CAAATATTCCTTGTGAATTC+AGG	+	chr7_4:28528008-28528027	None:intergenic	30.0%
GAATATTTGAAGAAACATGG+TGG	-	chr7_4:28528019-28528038	Msa1148400:CDS	30.0%
AGAAAGAAATGAAAGTGACA+TGG	-	chr7_4:28528052-28528071	Msa1148400:CDS	30.0%
CAATAAAACCACAAGTAGAA+AGG	-	chr7_4:28528260-28528279	Msa1148400:CDS	30.0%
CACATGCAAAATCTTATGTT+TGG	-	chr7_4:28528353-28528372	Msa1148400:intron	30.0%
TATTCTCATCATGATGTGAT+TGG	-	chr7_4:28528396-28528415	Msa1148400:intron	30.0%
GATATACAACAATTCCAAAG+TGG	-	chr7_4:28528447-28528466	Msa1148400:CDS	30.0%
GTGAAAACAACTATACACAA+TGG	+	chr7_4:28529132-28529151	None:intergenic	30.0%
AATGCATTCAAACTGTTGTT+TGG	+	chr7_4:28529352-28529371	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	28527901	28530328	28527901	ID=Msa1148400;Name=Msa1148400
chr7_4	mRNA	28527901	28530328	28527901	ID=Msa1148400-mRNA-1;Parent=Msa1148400;Name=Msa1148400-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0\|0.83\|0.57\|1\|0.83\|0.57\|7\|0\|531
chr7_4	exon	28527901	28528104	28527901	ID=Msa1148400-mRNA-1:exon:7054;Parent=Msa1148400-mRNA-1
chr7_4	exon	28528198	28528348	28528198	ID=Msa1148400-mRNA-1:exon:7053;Parent=Msa1148400-mRNA-1
chr7_4	exon	28528439	28528676	28528439	ID=Msa1148400-mRNA-1:exon:7052;Parent=Msa1148400-mRNA-1
chr7_4	exon	28528850	28529060	28528850	ID=Msa1148400-mRNA-1:exon:7051;Parent=Msa1148400-mRNA-1
chr7_4	exon	28529136	28529284	28529136	ID=Msa1148400-mRNA-1:exon:7050;Parent=Msa1148400-mRNA-1
chr7_4	exon	28529376	28529495	28529376	ID=Msa1148400-mRNA-1:exon:7049;Parent=Msa1148400-mRNA-1
chr7_4	exon	28529809	28530328	28529809	ID=Msa1148400-mRNA-1:exon:7048;Parent=Msa1148400-mRNA-1
chr7_4	CDS	28529809	28530328	28529809	ID=Msa1148400-mRNA-1:cds;Parent=Msa1148400-mRNA-1
chr7_4	CDS	28529376	28529495	28529376	ID=Msa1148400-mRNA-1:cds;Parent=Msa1148400-mRNA-1
chr7_4	CDS	28529136	28529284	28529136	ID=Msa1148400-mRNA-1:cds;Parent=Msa1148400-mRNA-1
chr7_4	CDS	28528850	28529060	28528850	ID=Msa1148400-mRNA-1:cds;Parent=Msa1148400-mRNA-1
chr7_4	CDS	28528439	28528676	28528439	ID=Msa1148400-mRNA-1:cds;Parent=Msa1148400-mRNA-1
chr7_4	CDS	28528198	28528348	28528198	ID=Msa1148400-mRNA-1:cds;Parent=Msa1148400-mRNA-1
chr7_4	CDS	28527901	28528104	28527901	ID=Msa1148400-mRNA-1:cds;Parent=Msa1148400-mRNA-1

Gene Sequence

>Msa1148400

ATGAAATTTGAATATACTAATACTTTTCAAGGTGAACACAATGGAGAAGGAAATAATGTTCTATTTTATACGAGACCAAACGACAAGGAATTATATATATATCACCTGAATTCACAAGGAATATTTGAAGAAACATGGTGGGATGATAAGAAGAAAGAAATGAAAGTGACATGGAAAAGCCAAAACGCAGAATGTGATGTTTATGGTCTATGTGGAGAATTTACAAGCTGTAATTCATTGAGCTCACCAGTATGCAGTTGTTTGAAAGGATTTGAACCCAGAAATACTCGTGAATGGAATAGAAACAACTGGACTGGCGGGTGTGTTAGGCGGAGACCTTTGCAGTGTGAAAGGATCAGCAATAAAACCACAAGTAGAAAGGAAGACGGGTTTCTGAAACTACAGATGGTTAAAGTTCCAGATTTTGCTGAAGGTTTAGGAGTTACACCTGACACATGCAAAATCTTATGTTTGGAGAATTGCTCCTGTGTTGCGTATTCTCATCATGATGTGATTGGCTATACAGGGAGAAATACAGGGAGAAACAAGACAATCTTCGTCGCAAGTGCAGTGATAATTGGAACTCTTATTATTGTCATTTGCGCATATATCACGTGGAGAAGGACATCAAACCACCCAGCTAATTCTAGTGACAACATAATTGGAGAAATGTCGCAAGATAAACTACAAGAGCTATTAAAATTTGATTTTGAAAAAGTTGCAACTGCCACAAACAACTTTGACCTGTCGAACAAACTTGGACAGGGTGGTTTTGGTCCCGTATACAAGGGGAAACTGAAAGATGGTCGAGAAATTGCAGTTAAAAGACTTTCTAGAGCATCTGGACAAGGGTTGGAGGAATTCATGAACGAAGTATTGGTGATTTGTAAGCTTCAACACCGCAACCTCGTAAGACTTATTGGCTGCTGTGTTGAAGGTGATGAAAAGATGTTGATGTATGAATACATGCCAAACAACAGTTTGAATGCATTCATCTTTGATCCATCAAAAAACAAACTCCTAGATTGGAGGACACGGTATAGCATTATAGAAGGAATTGCTCGAGGGTTGCTATATCTTCACAGAGATTCTAGGCTAAGAATTATTCATAGAGATTTGAAGGCAAGTAATGTCTTGTTAGATGAGGAGTTGAATCCAAAAATTTCCGACTTTGGTATGGCTAGAATCTTTGGAGGAGGTGAAGATCAGGCCAATACTAGAAGGATTGTCGGAACTTATGGTTACATGTCTCCTGAATATGCAATGCAAGGATTATTTTCGGAGAAATCGGATGTTTTTAGCTTTGGTGTATTGCTACTAGAGATTGTTATCGGGAGAAGAAACTCAAGTTTTTATGACGATGAGCATAATCTGAGCCTTTTAGGATTCGTATGGACACAATGGAGGGAAGACAATATTTTGTCTCTAATAGATCAAGAAATATATGATCATAGTCATCATAATTATATTTCAAGGTGCATACATATAGGACTATTGTGTGCACAAGAACGTGCCGGAGATAGGCCCACTATGGCTGCAGTAATTTCTATGCTTAACAGTGAAACTTCATTACTTCCTCCTCCTAGTAAACCT

Protein sequence

>Msa1148400

MKFEYTNTFQGEHNGEGNNVLFYTRPNDKELYIYHLNSQGIFEETWWDDKKKEMKVTWKSQNAECDVYGLCGEFTSCNSLSSPVCSCLKGFEPRNTREWNRNNWTGGCVRRRPLQCERISNKTTSRKEDGFLKLQMVKVPDFAEGLGVTPDTCKILCLENCSCVAYSHHDVIGYTGRNTGRNKTIFVASAVIIGTLIIVICAYITWRRTSNHPANSSDNIIGEMSQDKLQELLKFDFEKVATATNNFDLSNKLGQGGFGPVYKGKLKDGREIAVKRLSRASGQGLEEFMNEVLVICKLQHRNLVRLIGCCVEGDEKMLMYEYMPNNSLNAFIFDPSKNKLLDWRTRYSIIEGIARGLLYLHRDSRLRIIHRDLKASNVLLDEELNPKISDFGMARIFGGGEDQANTRRIVGTYGYMSPEYAMQGLFSEKSDVFSFGVLLLEIVIGRRNSSFYDDEHNLSLLGFVWTQWREDNILSLIDQEIYDHSHHNYISRCIHIGLLCAQERAGDRPTMAAVISMLNSETSLLPPPSKP