AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1149050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1149050 MtrunA17_Chr7g0237921 92.113 355 21 2 1 355 1 348 0.0 555
Msa1149050 MtrunA17_Chr4g0010151 48.052 385 125 16 1 342 1 353 9.19e-88 268
Msa1149050 MtrunA17_Chr4g0031411 43.343 353 156 11 1 331 1 331 2.35e-72 228
Msa1149050 MtrunA17_Chr7g0223311 55.479 146 57 4 1 142 1 142 1.25e-44 157
Msa1149050 MtrunA17_Chr2g0328021 40.076 262 118 11 1 260 1 225 3.64e-44 154
Msa1149050 MtrunA17_Chr8g0374251 56.000 100 38 1 77 170 69 168 1.12e-31 122
Msa1149050 MtrunA17_Chr3g0126201 63.750 80 29 0 57 136 32 111 1.14e-30 119
Msa1149050 MtrunA17_Chr3g0127201 66.667 75 21 1 57 131 12 82 5.03e-30 116
Msa1149050 MtrunA17_Chr5g0411951 59.633 109 39 1 73 181 40 143 9.62e-30 116
Msa1149050 MtrunA17_Chr3g0117601 63.636 88 25 3 43 126 27 111 1.23e-29 116
Msa1149050 MtrunA17_Chr2g0326001 78.689 61 13 0 70 130 34 94 1.35e-29 115
Msa1149050 MtrunA17_Chr8g0367161 73.438 64 17 0 72 135 28 91 2.20e-29 115
Msa1149050 MtrunA17_Chr4g0030051 81.356 59 11 0 70 128 35 93 3.92e-29 114
Msa1149050 MtrunA17_Chr8g0374111 60.000 105 25 4 55 151 17 112 8.87e-29 111
Msa1149050 MtrunA17_Chr2g0282441 67.606 71 22 1 58 127 6 76 1.32e-28 113
Msa1149050 MtrunA17_Chr4g0061761 90.000 50 5 0 77 126 41 90 4.63e-28 111
Msa1149050 MtrunA17_Chr8g0341671 73.913 69 15 2 68 136 13 78 5.85e-28 108
Msa1149050 MtrunA17_Chr1g0177251 80.357 56 11 0 75 130 11 66 6.74e-28 110
Msa1149050 MtrunA17_Chr8g0346551 71.014 69 16 1 78 142 26 94 6.89e-28 110
Msa1149050 MtrunA17_Chr2g0282511 89.583 48 5 0 77 124 46 93 1.03e-26 107
Msa1149050 MtrunA17_Chr2g0282051 65.278 72 16 2 69 132 31 101 1.87e-26 106
Msa1149050 MtrunA17_Chr1g0188851 52.439 82 37 1 53 132 18 99 4.72e-26 105
Msa1149050 MtrunA17_Chr4g0047761 81.818 55 9 1 78 132 74 127 5.37e-26 106
Msa1149050 MtrunA17_Chr2g0308011 87.500 48 6 0 77 124 38 85 1.02e-25 102
Msa1149050 MtrunA17_Chr6g0453321 71.429 56 16 0 78 133 138 193 2.58e-23 100
Msa1149050 MtrunA17_Chr7g0254161 42.982 114 54 1 77 179 102 215 2.71e-23 100
Msa1149050 MtrunA17_Chr3g0092111 68.421 57 18 0 78 134 138 194 4.82e-23 99.8
Msa1149050 MtrunA17_Chr2g0283811 53.247 77 33 1 78 154 49 122 6.61e-23 93.6
Msa1149050 MtrunA17_Chr7g0217581 61.194 67 26 0 78 144 234 300 6.74e-23 99.8
Msa1149050 MtrunA17_Chr8g0355261 68.519 54 17 0 78 131 120 173 7.91e-23 99.0
Msa1149050 MtrunA17_Chr6g0460721 66.667 54 18 0 77 130 90 143 4.74e-22 95.5
Msa1149050 MtrunA17_Chr4g0042481 69.231 52 16 0 78 129 124 175 5.52e-22 96.7
Msa1149050 MtrunA17_Chr5g0417571 61.290 62 24 0 78 139 140 201 1.98e-21 93.6
Msa1149050 MtrunA17_Chr5g0417561 58.667 75 25 1 78 152 121 189 2.03e-21 94.0
Msa1149050 MtrunA17_Chr5g0417551 66.102 59 20 0 78 136 129 187 7.40e-21 92.4
Msa1149050 MtrunA17_Chr6g0460731 57.377 61 24 1 71 129 89 149 1.01e-20 92.0
Msa1149050 MtrunA17_Chr5g0417601 69.231 52 16 0 78 129 142 193 1.40e-20 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1149050 AT3G55370.2 38.120 383 156 18 1 340 1 345 4.01e-52 176
Msa1149050 AT3G55370.1 38.525 366 147 17 1 340 1 314 3.84e-51 173
Msa1149050 AT3G55370.3 40.385 312 123 14 48 340 92 359 3.27e-46 161
Msa1149050 AT2G37590.1 40.580 276 107 9 1 240 1 255 3.44e-45 158
Msa1149050 AT5G02460.1 86.842 76 10 0 55 130 74 149 7.94e-44 156
Msa1149050 AT1G07640.1 73.418 79 21 0 58 136 3 81 1.05e-36 134
Msa1149050 AT1G07640.2 73.418 79 21 0 58 136 59 137 1.54e-36 135
Msa1149050 AT1G07640.3 73.418 79 21 0 58 136 67 145 2.04e-36 135
Msa1149050 AT2G28810.1 46.711 152 49 6 1 127 1 145 2.33e-34 129
Msa1149050 AT5G60200.1 69.512 82 22 1 73 151 52 133 1.72e-33 125
Msa1149050 AT2G28810.2 74.648 71 17 1 58 127 58 128 3.17e-32 123
Msa1149050 AT5G60850.1 61.957 92 32 1 63 154 38 126 3.87e-32 122
Msa1149050 AT3G52440.1 72.464 69 19 0 73 141 22 90 5.01e-31 118
Msa1149050 AT3G52440.2 72.464 69 19 0 73 141 41 109 7.23e-31 118
Msa1149050 AT3G45610.1 68.571 70 22 0 73 142 37 106 1.27e-30 117
Msa1149050 AT3G21270.1 49.635 137 56 3 77 213 30 153 4.82e-30 114
Msa1149050 AT1G51700.1 67.500 80 24 2 69 147 27 105 5.02e-30 114
Msa1149050 AT5G65590.1 54.639 97 37 2 35 131 7 96 7.05e-30 117
Msa1149050 AT1G28310.1 83.929 56 9 0 77 132 28 83 3.06e-29 115
Msa1149050 AT1G28310.2 83.929 56 9 0 77 132 52 107 4.95e-29 115
Msa1149050 AT1G21340.1 61.176 85 31 1 78 162 40 122 6.72e-29 112
Msa1149050 AT4G21050.1 40.476 168 72 4 74 213 22 189 6.94e-29 111
Msa1149050 AT5G62940.1 47.656 128 60 4 1 126 1 123 5.93e-28 112
Msa1149050 AT5G66940.1 51.351 111 42 4 37 145 2 102 1.02e-27 108
Msa1149050 AT3G61850.1 45.985 137 55 5 10 133 1 131 1.04e-27 110
Msa1149050 AT3G50410.1 56.818 88 35 2 49 133 1 88 1.08e-27 109
Msa1149050 AT4G38000.1 61.446 83 31 1 70 152 35 116 1.31e-27 109
Msa1149050 AT3G61850.2 54.455 101 41 3 38 133 19 119 1.57e-27 109
Msa1149050 AT3G61850.3 54.455 101 41 3 38 133 19 119 1.57e-27 109
Msa1149050 AT3G61850.4 45.985 137 55 5 10 133 1 131 1.61e-27 110
Msa1149050 AT3G61850.5 54.455 101 41 3 38 133 19 119 2.16e-27 110
Msa1149050 AT4G24060.1 72.131 61 17 0 70 130 47 107 2.80e-27 110
Msa1149050 AT4G00940.1 74.576 59 14 1 72 130 65 122 6.42e-26 105
Msa1149050 AT4G00940.3 74.576 59 14 1 72 130 65 122 6.42e-26 105
Msa1149050 AT4G00940.2 74.576 59 14 1 72 130 65 122 6.42e-26 105
Msa1149050 AT2G28510.1 38.710 248 105 10 75 322 47 247 2.65e-25 103
Msa1149050 AT2G46590.1 77.358 53 10 1 73 123 63 115 9.54e-24 100
Msa1149050 AT2G46590.2 77.358 53 10 1 73 123 75 127 1.09e-23 100
Msa1149050 AT1G64620.1 47.020 151 59 5 70 213 43 179 1.24e-23 100
Msa1149050 AT5G62430.1 73.077 52 14 0 78 129 56 107 1.65e-23 99.4
Msa1149050 AT3G47500.1 63.636 66 24 0 78 143 112 177 2.84e-23 100
Msa1149050 AT1G47655.1 78.723 47 10 0 78 124 31 77 1.78e-22 94.4
Msa1149050 AT2G34140.1 71.154 52 15 0 78 129 60 111 3.22e-22 92.4
Msa1149050 AT1G26790.1 69.231 52 16 0 78 129 113 164 3.31e-22 96.7
Msa1149050 AT1G29160.1 71.154 52 15 0 78 129 64 115 3.60e-22 92.4
Msa1149050 AT4G21040.1 72.727 55 14 1 78 132 27 80 5.51e-22 93.6
Msa1149050 AT5G39660.3 71.154 52 15 0 78 129 140 191 1.10e-21 96.3
Msa1149050 AT5G39660.1 71.154 52 15 0 78 129 140 191 1.10e-21 96.3
Msa1149050 AT5G39660.2 71.154 52 15 0 78 129 140 191 1.10e-21 96.3
Msa1149050 AT1G69570.1 69.231 52 16 0 78 129 134 185 2.99e-21 94.4
Msa1149050 AT4G21080.1 53.165 79 33 1 78 156 27 101 7.82e-21 90.9
Msa1149050 AT4G21030.1 42.667 75 43 0 74 148 19 93 1.16e-13 69.3

Find 136 sgRNAs with CRISPR-Local

Find 329 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCAACCGGGACACTTCTA+AGG 0.192890 7_4:-29872997 None:intergenic
TACTTCTCTCCCACCATATA+TGG 0.222301 7_4:+29872497 Msa1149050:CDS
TTCCAATATTGTTCACTATT+AGG 0.263046 7_4:-29873598 None:intergenic
GTAAAGGTTGAAAGGTTTCT+AGG 0.265157 7_4:-29872438 None:intergenic
AAATAATTCTAGTGTGAAAA+TGG 0.280136 7_4:+29873501 Msa1149050:CDS
ATCTCATCGGTGGTGGCGTT+GGG 0.300347 7_4:-29873135 None:intergenic
TTCCATATCGGAAGCTCTTT+AGG 0.302834 7_4:+29873253 Msa1149050:CDS
GGTGATCACCTAAGTGAAAT+AGG 0.308942 7_4:+29873181 Msa1149050:CDS
AGTTTGAAACTCCACATATA+TGG 0.315984 7_4:+29873790 Msa1149050:three_prime_UTR
ATCCTAATAGTGAACAATAT+TGG 0.316935 7_4:+29873596 Msa1149050:CDS
GAATCAATCTAGACAATTCT+TGG 0.322625 7_4:+29873528 Msa1149050:CDS
GCAAGGCTTGTCGAAGGTAT+TGG 0.355195 7_4:+29872960 Msa1149050:CDS
CACCTAAAGAGCTTCCGATA+TGG 0.360566 7_4:-29873255 None:intergenic
AGTTGTTTGTAGAGCTAGTT+TGG 0.376308 7_4:-29873093 None:intergenic
TTCTTCTAATTATTAGGTTT+AGG 0.379272 7_4:+29873948 Msa1149050:three_prime_UTR
AGAGAGGGATCAAAGGGTTA+AGG 0.394747 7_4:-29872294 None:intergenic
CTTCGGCAACCACCGCCAAC+CGG 0.399525 7_4:-29873010 None:intergenic
CATGAGGTTGCGATGGTGTC+AGG 0.399816 7_4:-29872784 None:intergenic
ACCTGTTGATGCCAGTTGGC+TGG 0.401216 7_4:-29872522 None:intergenic
TAAACCATGGTTATTCCTCT+TGG 0.404319 7_4:-29872474 None:intergenic
CAGTTGGCTGGATCCATATA+TGG 0.404780 7_4:-29872510 None:intergenic
AAGAAAGAAAGAAAGAAGAG+AGG 0.409856 7_4:-29872310 None:intergenic
AGAAAGAAGAGAGGGATCAA+AGG 0.410619 7_4:-29872301 None:intergenic
AATCTCATCGGTGGTGGCGT+TGG 0.416328 7_4:-29873136 None:intergenic
GGAGACTTAAATTTCCATAT+CGG 0.426564 7_4:+29873241 Msa1149050:CDS
GTCTTGAGATTAATAATAGT+AGG 0.430412 7_4:-29873765 None:intergenic
TCCAGCCAACTGGCATCAAC+AGG 0.444071 7_4:+29872521 Msa1149050:CDS
GGATCAAAGGGTTAAGGAGG+TGG 0.451646 7_4:-29872288 None:intergenic
CTACTACAAATTCCAACTAT+AGG 0.452160 7_4:+29873692 Msa1149050:CDS
AAGGGAAATTGATGAGTTTG+TGG 0.457842 7_4:-29873358 None:intergenic
CCGCTACCACCACCTTCAAA+TGG 0.459662 7_4:-29873424 None:intergenic
AGTGAAATAGGACTAAACTA+TGG 0.460875 7_4:+29873193 Msa1149050:CDS
GCCCTATCTGCCATCGAACC+TGG 0.462544 7_4:-29872821 None:intergenic
TCCAGTCCAATGGGAGGTGT+TGG 0.464011 7_4:+29873220 Msa1149050:CDS
GCCACCACCGATGAGATTCA+TGG 0.464462 7_4:+29873141 Msa1149050:CDS
GAAAGAAGAGAGGGATCAAA+GGG 0.466314 7_4:-29872300 None:intergenic
AATCAATCTAGACAATTCTT+GGG 0.469852 7_4:+29873529 Msa1149050:CDS
GACCAGGTTCGATGGCAGAT+AGG 0.475722 7_4:+29872819 Msa1149050:CDS
TGCGTATGGTGGTGTGTCTA+AGG 0.477118 7_4:+29873471 Msa1149050:CDS
CTTAGAAGTGTCCCGGTTGG+CGG 0.481122 7_4:+29872998 Msa1149050:CDS
CATATATGGATCCAGCCAAC+TGG 0.482920 7_4:+29872511 Msa1149050:CDS
ATGGCTCCTTTGCATCATCT+AGG 0.483090 7_4:+29873160 Msa1149050:CDS
TAAGATAGAATGAAAACCCT+AGG 0.483769 7_4:+29873833 Msa1149050:three_prime_UTR
ATAAAGTTATGTCCTATAGT+TGG 0.484745 7_4:-29873704 None:intergenic
AGAATCTAAGTAACATCCCT+AGG 0.486444 7_4:-29873850 None:intergenic
AAAGAGAGCTATCGGGGAAT+GGG 0.486965 7_4:+29872172 Msa1149050:five_prime_UTR
TAAAGGTTGAAAGGTTTCTA+GGG 0.487752 7_4:-29872437 None:intergenic
ACCAGGTTCGATGGCAGATA+GGG 0.488883 7_4:+29872820 Msa1149050:CDS
GAAGGTGGTGGTAGCGGTGG+TGG 0.489455 7_4:+29873430 Msa1149050:CDS
AGGTTCGATGGTTGAGGCTG+TGG 0.490154 7_4:-29872764 None:intergenic
TTCGACAAGCCTTGCAGAAG+TGG 0.490902 7_4:-29872952 None:intergenic
CATTGCTCAAAATTGCCAAC+AGG 0.493075 7_4:-29873328 None:intergenic
AGAAGTGTCCCGGTTGGCGG+TGG 0.494695 7_4:+29873001 Msa1149050:CDS
CCATTTGAAGGTGGTGGTAG+CGG 0.496097 7_4:+29873424 Msa1149050:CDS
AGAGCTAGCCGGCGACTTGG+AGG 0.502693 7_4:-29873065 None:intergenic
GAGGGATCAAAGGGTTAAGG+AGG 0.504853 7_4:-29872291 None:intergenic
TCTTGAGATTAATAATAGTA+GGG 0.508512 7_4:-29873764 None:intergenic
AAAAGAGAGCTATCGGGGAA+TGG 0.509451 7_4:+29872171 Msa1149050:five_prime_UTR
TTAGGTGGAGGCGTTGGTAA+CGG 0.512562 7_4:+29873271 Msa1149050:CDS
AGTTTAGTCCTATTTCACTT+AGG 0.513240 7_4:-29873189 None:intergenic
AACGGTGGTGTCAACGGTGG+TGG 0.514797 7_4:+29873289 Msa1149050:CDS
AGAAAGAAAGAAAGAAGAGA+GGG 0.518141 7_4:-29872309 None:intergenic
GATGGTTGAGGCTGTGGTGG+TGG 0.518267 7_4:-29872758 None:intergenic
GGATTGTTGTATCCATTTGA+AGG 0.522126 7_4:+29873412 Msa1149050:CDS
TGGAATGCTTCTGCTGGTGG+TGG 0.522977 7_4:+29873616 Msa1149050:CDS
TCCAACACCTCCCATTGGAC+TGG 0.527498 7_4:-29873221 None:intergenic
TGCGATGGTGTCAGGTTCGA+TGG 0.530120 7_4:-29872776 None:intergenic
AGCTTCTAAAGTTGACAAGA+AGG 0.532028 7_4:-29873377 None:intergenic
CGGTGCCCTTAGAAGTGTCC+CGG 0.534033 7_4:+29872991 Msa1149050:CDS
GTAGCAAAACTTTGTGTTGG+TGG 0.539364 7_4:-29872900 None:intergenic
GTAGTAGCAAAACTTTGTGT+TGG 0.542073 7_4:-29872903 None:intergenic
TTCGGCAACCACCGCCAACC+GGG 0.543263 7_4:-29873009 None:intergenic
AAAGTACTAAACCATATATG+TGG 0.544412 7_4:-29873801 None:intergenic
AGCTCTTTAGGTGGAGGCGT+TGG 0.544707 7_4:+29873265 Msa1149050:CDS
CTGACACCATCGCAACCTCA+TGG 0.545229 7_4:+29872785 Msa1149050:CDS
GAGAGCTATCGGGGAATGGG+AGG 0.546285 7_4:+29872175 Msa1149050:five_prime_UTR
CTCGATCAGACCAGGTTCGA+TGG 0.546965 7_4:+29872811 Msa1149050:CDS
GCCATGAATCTCATCGGTGG+TGG 0.547604 7_4:-29873142 None:intergenic
CATGGAGGCTCGATCAGACC+AGG 0.549550 7_4:+29872803 Msa1149050:CDS
TGTCGAAGGTATTGGACAAA+GGG 0.551224 7_4:+29872968 Msa1149050:CDS
TTGTATCCATTTGAAGGTGG+TGG 0.552400 7_4:+29873418 Msa1149050:CDS
GCAGCCACGCCACTTCTGCA+AGG 0.554356 7_4:+29872943 Msa1149050:CDS
ATCGGAAGCTCTTTAGGTGG+AGG 0.559518 7_4:+29873259 Msa1149050:CDS
ATTTACCTGTTGATGCCAGT+TGG 0.559637 7_4:-29872526 None:intergenic
GTTGGTAACGGTGGTGTCAA+CGG 0.562708 7_4:+29873283 Msa1149050:CDS
GCCCTTAGAAGTGTCCCGGT+TGG 0.564826 7_4:+29872995 Msa1149050:CDS
CAATATTGGAATGCTTCTGC+TGG 0.566637 7_4:+29873610 Msa1149050:CDS
TTCGATGGTTGAGGCTGTGG+TGG 0.570012 7_4:-29872761 None:intergenic
GTTGGGGAACTGAGTACCGC+AGG 0.570283 7_4:-29873118 None:intergenic
CATATCGGAAGCTCTTTAGG+TGG 0.571600 7_4:+29873256 Msa1149050:CDS
AAGTCTCCAACACCTCCCAT+TGG 0.574092 7_4:-29873226 None:intergenic
TGTGGTGGTGGAATAAGTAG+AGG 0.574363 7_4:-29872746 None:intergenic
GATGGCAGATAGGGCAAGGA+TGG 0.575021 7_4:+29872829 Msa1149050:CDS
TAGACACACCACCATACGCA+TGG 0.575174 7_4:-29873468 None:intergenic
TCTCATCGGTGGTGGCGTTG+GGG 0.576658 7_4:-29873134 None:intergenic
GCCAACCGGGACACTTCTAA+GGG 0.580076 7_4:-29872996 None:intergenic
GTTTGGCGGTCAGAGCTAGC+CGG 0.580677 7_4:-29873076 None:intergenic
TTAGAAGCTTCTTCTTCACA+TGG 0.581519 7_4:+29873391 Msa1149050:CDS
TATTGGAATGCTTCTGCTGG+TGG 0.586587 7_4:+29873613 Msa1149050:CDS
GTGGTTGCCGAAGAAACAAA+AGG 0.588845 7_4:+29873020 Msa1149050:CDS
GCTTCTAAAGTTGACAAGAA+GGG 0.589264 7_4:-29873376 None:intergenic
TTGTCGAAGGTATTGGACAA+AGG 0.589503 7_4:+29872967 Msa1149050:CDS
TGTTTGTAGAGCTAGTTTGG+CGG 0.589509 7_4:-29873090 None:intergenic
TAACAACACCTCCAAGTCGC+CGG 0.589920 7_4:+29873057 Msa1149050:CDS
CAAGCCTTGCAGAAGTGGCG+TGG 0.593138 7_4:-29872947 None:intergenic
GCAGCAACAAAATCATCAAG+TGG 0.596192 7_4:+29872703 Msa1149050:intron
AAGATAGAATGAAAACCCTA+GGG 0.597350 7_4:+29873834 Msa1149050:three_prime_UTR
TTTGAAGGTGGTGGTAGCGG+TGG 0.603652 7_4:+29873427 Msa1149050:CDS
GAGCCTCCATGAGGTTGCGA+TGG 0.604696 7_4:-29872791 None:intergenic
AGGAGTGTTGACACTAGTGT+TGG 0.605835 7_4:-29872726 None:intergenic
AGAGCTATCGGGGAATGGGA+GGG 0.607461 7_4:+29872176 Msa1149050:five_prime_UTR
GGTGTCAGGTTCGATGGTTG+AGG 0.608734 7_4:-29872770 None:intergenic
TTGGCTGGATCCATATATGG+TGG 0.609224 7_4:-29872507 None:intergenic
GTTCGATGGCAGATAGGGCA+AGG 0.614474 7_4:+29872825 Msa1149050:CDS
TTGTTGTATCCATTTGAAGG+TGG 0.620036 7_4:+29873415 Msa1149050:CDS
TGATCACCTAGATGATGCAA+AGG 0.622493 7_4:-29873166 None:intergenic
TGGCTGGATCCATATATGGT+GGG 0.628650 7_4:-29872506 None:intergenic
GGTGGAGGCGTTGGTAACGG+TGG 0.630519 7_4:+29873274 Msa1149050:CDS
GAATCTAAGTAACATCCCTA+GGG 0.631813 7_4:-29873849 None:intergenic
AAAGGAGCCATGAATCTCAT+CGG 0.638198 7_4:-29873148 None:intergenic
ACTTCTGCAAGGCTTGTCGA+AGG 0.638852 7_4:+29872954 Msa1149050:CDS
GCTGTAGAAAGATCTGTCCA+AGG 0.641214 7_4:-29873652 None:intergenic
CGAAGGTATTGGACAAAGGG+CGG 0.642797 7_4:+29872971 Msa1149050:CDS
GTGGTGCTACTAATTCACCT+TGG 0.642878 7_4:+29873635 Msa1149050:CDS
AGACACACCACCATACGCAT+GGG 0.645170 7_4:-29873467 None:intergenic
AATGATCAATCCCATGCGTA+TGG 0.649437 7_4:+29873457 Msa1149050:CDS
ACAAAAGATAAAGCTCCAAG+AGG 0.661855 7_4:+29872459 Msa1149050:five_prime_UTR
GATCAATCCCATGCGTATGG+TGG 0.672453 7_4:+29873460 Msa1149050:CDS
CTCTACAAACAACTCTCCTG+CGG 0.682618 7_4:+29873102 Msa1149050:CDS
GTGTTGGTGGAATCACACCT+TGG 0.687306 7_4:-29872887 None:intergenic
GGTCTGATCGAGCCTCCATG+AGG 0.689453 7_4:-29872800 None:intergenic
GGAGCCATGAATCTCATCGG+TGG 0.692277 7_4:-29873145 None:intergenic
AGCTCCAAGAGGAATAACCA+TGG 0.692985 7_4:+29872470 Msa1149050:exon
GGTAACGGTGGTGTCAACGG+TGG 0.699310 7_4:+29873286 Msa1149050:CDS
TGGGAGAGAAGTATAAACCA+TGG 0.714065 7_4:-29872487 None:intergenic
ACACCATCGCAACCTCATGG+AGG 0.720835 7_4:+29872788 Msa1149050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGGAAATAAAAATTAA+TGG - chr7_4:29872682-29872701 None:intergenic 10.0%
!! GAAAAATTAAACTAAAAAAA+AGG - chr7_4:29873931-29873950 None:intergenic 10.0%
!!! TAATTTTTCTTCTAATTATT+AGG + chr7_4:29873942-29873961 Msa1149050:three_prime_UTR 10.0%
!! AAAAAGGAAATAAAAATTAA+TGG - chr7_4:29872682-29872701 None:intergenic 10.0%
!! GAAAAATTAAACTAAAAAAA+AGG - chr7_4:29873931-29873950 None:intergenic 10.0%
!!! TAATTTTTCTTCTAATTATT+AGG + chr7_4:29873942-29873961 Msa1149050:three_prime_UTR 10.0%
!! GATACATAAAATCAAATATT+TGG - chr7_4:29872655-29872674 None:intergenic 15.0%
!! GATACATAAAATCAAATATT+TGG - chr7_4:29872655-29872674 None:intergenic 15.0%
!! AAATAATTCTAGTGTGAAAA+TGG + chr7_4:29873501-29873520 Msa1149050:CDS 20.0%
!! TCTTGAGATTAATAATAGTA+GGG - chr7_4:29873767-29873786 None:intergenic 20.0%
!! TTCTTCTAATTATTAGGTTT+AGG + chr7_4:29873948-29873967 Msa1149050:three_prime_UTR 20.0%
!! AAATAATTCTAGTGTGAAAA+TGG + chr7_4:29873501-29873520 Msa1149050:CDS 20.0%
!! TCTTGAGATTAATAATAGTA+GGG - chr7_4:29873767-29873786 None:intergenic 20.0%
! TGATGAGAAGCTAAAAATAA+AGG - chr7_4:29872268-29872287 None:intergenic 25.0%
!! TATCTTTTGTAAAGGTTGAA+AGG - chr7_4:29872449-29872468 None:intergenic 25.0%
! ATTTGGTATATTCAAGAGAA+GGG - chr7_4:29872638-29872657 None:intergenic 25.0%
! TATTTGGTATATTCAAGAGA+AGG - chr7_4:29872639-29872658 None:intergenic 25.0%
!!! ATTTAGACCTTTTGTTTCTT+CGG - chr7_4:29873030-29873049 None:intergenic 25.0%
! AATCAATCTAGACAATTCTT+GGG + chr7_4:29873529-29873548 Msa1149050:CDS 25.0%
! ATCCTAATAGTGAACAATAT+TGG + chr7_4:29873596-29873615 Msa1149050:CDS 25.0%
! TTCCAATATTGTTCACTATT+AGG - chr7_4:29873601-29873620 None:intergenic 25.0%
! ATAAAGTTATGTCCTATAGT+TGG - chr7_4:29873707-29873726 None:intergenic 25.0%
! GTCTTGAGATTAATAATAGT+AGG - chr7_4:29873768-29873787 None:intergenic 25.0%
!! AAAGTACTAAACCATATATG+TGG - chr7_4:29873804-29873823 None:intergenic 25.0%
!!! CTTATTTTTCCACTTTTTTG+AGG + chr7_4:29873871-29873890 Msa1149050:three_prime_UTR 25.0%
! AGATTAAAACCTCAAAAAAG+TGG - chr7_4:29873883-29873902 None:intergenic 25.0%
! TGATGAGAAGCTAAAAATAA+AGG - chr7_4:29872268-29872287 None:intergenic 25.0%
!! TATCTTTTGTAAAGGTTGAA+AGG - chr7_4:29872449-29872468 None:intergenic 25.0%
! ATTTGGTATATTCAAGAGAA+GGG - chr7_4:29872638-29872657 None:intergenic 25.0%
! TATTTGGTATATTCAAGAGA+AGG - chr7_4:29872639-29872658 None:intergenic 25.0%
!!! ATTTAGACCTTTTGTTTCTT+CGG - chr7_4:29873030-29873049 None:intergenic 25.0%
! AATCAATCTAGACAATTCTT+GGG + chr7_4:29873529-29873548 Msa1149050:CDS 25.0%
! ATCCTAATAGTGAACAATAT+TGG + chr7_4:29873596-29873615 Msa1149050:CDS 25.0%
! TTCCAATATTGTTCACTATT+AGG - chr7_4:29873601-29873620 None:intergenic 25.0%
! ATAAAGTTATGTCCTATAGT+TGG - chr7_4:29873707-29873726 None:intergenic 25.0%
! GTCTTGAGATTAATAATAGT+AGG - chr7_4:29873768-29873787 None:intergenic 25.0%
!! AAAGTACTAAACCATATATG+TGG - chr7_4:29873804-29873823 None:intergenic 25.0%
!!! CTTATTTTTCCACTTTTTTG+AGG + chr7_4:29873871-29873890 Msa1149050:three_prime_UTR 25.0%
! AGATTAAAACCTCAAAAAAG+TGG - chr7_4:29873883-29873902 None:intergenic 25.0%
AAAACAAAAAGAGAGCTATC+GGG + chr7_4:29872165-29872184 Msa1149050:five_prime_UTR 30.0%
AGAAAGAAAGAAAGAAGAGA+GGG - chr7_4:29872312-29872331 None:intergenic 30.0%
AAGAAAGAAAGAAAGAAGAG+AGG - chr7_4:29872313-29872332 None:intergenic 30.0%
TAAAGGTTGAAAGGTTTCTA+GGG - chr7_4:29872440-29872459 None:intergenic 30.0%
!! TGGAGCTTTATCTTTTGTAA+AGG - chr7_4:29872457-29872476 None:intergenic 30.0%
ATCAACAGGTAAATCAACAA+GGG + chr7_4:29872535-29872554 Msa1149050:intron 30.0%
! TGATTTTGTTGCTGCAAAAA+AGG - chr7_4:29872698-29872717 None:intergenic 30.0%
AGTTTAGTCCTATTTCACTT+AGG - chr7_4:29873192-29873211 None:intergenic 30.0%
AGTGAAATAGGACTAAACTA+TGG + chr7_4:29873193-29873212 Msa1149050:CDS 30.0%
GGAGACTTAAATTTCCATAT+CGG + chr7_4:29873241-29873260 Msa1149050:CDS 30.0%
GAATCAATCTAGACAATTCT+TGG + chr7_4:29873528-29873547 Msa1149050:CDS 30.0%
CTACTACAAATTCCAACTAT+AGG + chr7_4:29873692-29873711 Msa1149050:CDS 30.0%
! AGTTTGAAACTCCACATATA+TGG + chr7_4:29873790-29873809 Msa1149050:three_prime_UTR 30.0%
TAAGATAGAATGAAAACCCT+AGG + chr7_4:29873833-29873852 Msa1149050:three_prime_UTR 30.0%
AAGATAGAATGAAAACCCTA+GGG + chr7_4:29873834-29873853 Msa1149050:three_prime_UTR 30.0%
AAAACAAAAAGAGAGCTATC+GGG + chr7_4:29872165-29872184 Msa1149050:five_prime_UTR 30.0%
AGAAAGAAAGAAAGAAGAGA+GGG - chr7_4:29872312-29872331 None:intergenic 30.0%
AAGAAAGAAAGAAAGAAGAG+AGG - chr7_4:29872313-29872332 None:intergenic 30.0%
TAAAGGTTGAAAGGTTTCTA+GGG - chr7_4:29872440-29872459 None:intergenic 30.0%
!! TGGAGCTTTATCTTTTGTAA+AGG - chr7_4:29872457-29872476 None:intergenic 30.0%
ATCAACAGGTAAATCAACAA+GGG + chr7_4:29872535-29872554 Msa1149050:intron 30.0%
! TGATTTTGTTGCTGCAAAAA+AGG - chr7_4:29872698-29872717 None:intergenic 30.0%
AGTTTAGTCCTATTTCACTT+AGG - chr7_4:29873192-29873211 None:intergenic 30.0%
AGTGAAATAGGACTAAACTA+TGG + chr7_4:29873193-29873212 Msa1149050:CDS 30.0%
GGAGACTTAAATTTCCATAT+CGG + chr7_4:29873241-29873260 Msa1149050:CDS 30.0%
GAATCAATCTAGACAATTCT+TGG + chr7_4:29873528-29873547 Msa1149050:CDS 30.0%
CTACTACAAATTCCAACTAT+AGG + chr7_4:29873692-29873711 Msa1149050:CDS 30.0%
! AGTTTGAAACTCCACATATA+TGG + chr7_4:29873790-29873809 Msa1149050:three_prime_UTR 30.0%
TAAGATAGAATGAAAACCCT+AGG + chr7_4:29873833-29873852 Msa1149050:three_prime_UTR 30.0%
AAGATAGAATGAAAACCCTA+GGG + chr7_4:29873834-29873853 Msa1149050:three_prime_UTR 30.0%
AAACAAAAAGAGAGCTATCG+GGG + chr7_4:29872166-29872185 Msa1149050:five_prime_UTR 35.0%
!!! GATTGTGTGTGTTTTGTTTG+TGG - chr7_4:29872377-29872396 None:intergenic 35.0%
GTAAAGGTTGAAAGGTTTCT+AGG - chr7_4:29872441-29872460 None:intergenic 35.0%
ACAAAAGATAAAGCTCCAAG+AGG + chr7_4:29872459-29872478 Msa1149050:five_prime_UTR 35.0%
TAAACCATGGTTATTCCTCT+TGG - chr7_4:29872477-29872496 None:intergenic 35.0%
CATCAACAGGTAAATCAACA+AGG + chr7_4:29872534-29872553 Msa1149050:intron 35.0%
TCAACAAGGGTTCTTCAATT+AGG + chr7_4:29872548-29872567 Msa1149050:intron 35.0%
! TTTTGTGATGGTTCTTGCAT+AGG - chr7_4:29872863-29872882 None:intergenic 35.0%
AACCATCACAAAAATGTCCA+AGG + chr7_4:29872870-29872889 Msa1149050:CDS 35.0%
GTAGTAGCAAAACTTTGTGT+TGG - chr7_4:29872906-29872925 None:intergenic 35.0%
AGTTGTTTGTAGAGCTAGTT+TGG - chr7_4:29873096-29873115 None:intergenic 35.0%
! TATGGTTTTTCCAGTCCAAT+GGG + chr7_4:29873211-29873230 Msa1149050:CDS 35.0%
! TCTACTTCGATTTTACCTGT+TGG + chr7_4:29873313-29873332 Msa1149050:CDS 35.0%
AAGGGAAATTGATGAGTTTG+TGG - chr7_4:29873361-29873380 None:intergenic 35.0%
!! GCTTCTAAAGTTGACAAGAA+GGG - chr7_4:29873379-29873398 None:intergenic 35.0%
!! AGCTTCTAAAGTTGACAAGA+AGG - chr7_4:29873380-29873399 None:intergenic 35.0%
TTAGAAGCTTCTTCTTCACA+TGG + chr7_4:29873391-29873410 Msa1149050:CDS 35.0%
GGATTGTTGTATCCATTTGA+AGG + chr7_4:29873412-29873431 Msa1149050:CDS 35.0%
TTGTTGTATCCATTTGAAGG+TGG + chr7_4:29873415-29873434 Msa1149050:CDS 35.0%
GAATCTAAGTAACATCCCTA+GGG - chr7_4:29873852-29873871 None:intergenic 35.0%
AGAATCTAAGTAACATCCCT+AGG - chr7_4:29873853-29873872 None:intergenic 35.0%
AAACAAAAAGAGAGCTATCG+GGG + chr7_4:29872166-29872185 Msa1149050:five_prime_UTR 35.0%
!!! GATTGTGTGTGTTTTGTTTG+TGG - chr7_4:29872377-29872396 None:intergenic 35.0%
GTAAAGGTTGAAAGGTTTCT+AGG - chr7_4:29872441-29872460 None:intergenic 35.0%
ACAAAAGATAAAGCTCCAAG+AGG + chr7_4:29872459-29872478 Msa1149050:five_prime_UTR 35.0%
TAAACCATGGTTATTCCTCT+TGG - chr7_4:29872477-29872496 None:intergenic 35.0%
CATCAACAGGTAAATCAACA+AGG + chr7_4:29872534-29872553 Msa1149050:intron 35.0%
TCAACAAGGGTTCTTCAATT+AGG + chr7_4:29872548-29872567 Msa1149050:intron 35.0%
! TTTTGTGATGGTTCTTGCAT+AGG - chr7_4:29872863-29872882 None:intergenic 35.0%
AACCATCACAAAAATGTCCA+AGG + chr7_4:29872870-29872889 Msa1149050:CDS 35.0%
GTAGTAGCAAAACTTTGTGT+TGG - chr7_4:29872906-29872925 None:intergenic 35.0%
AGTTGTTTGTAGAGCTAGTT+TGG - chr7_4:29873096-29873115 None:intergenic 35.0%
! TATGGTTTTTCCAGTCCAAT+GGG + chr7_4:29873211-29873230 Msa1149050:CDS 35.0%
! TCTACTTCGATTTTACCTGT+TGG + chr7_4:29873313-29873332 Msa1149050:CDS 35.0%
AAGGGAAATTGATGAGTTTG+TGG - chr7_4:29873361-29873380 None:intergenic 35.0%
!! GCTTCTAAAGTTGACAAGAA+GGG - chr7_4:29873379-29873398 None:intergenic 35.0%
!! AGCTTCTAAAGTTGACAAGA+AGG - chr7_4:29873380-29873399 None:intergenic 35.0%
TTAGAAGCTTCTTCTTCACA+TGG + chr7_4:29873391-29873410 Msa1149050:CDS 35.0%
GGATTGTTGTATCCATTTGA+AGG + chr7_4:29873412-29873431 Msa1149050:CDS 35.0%
TTGTTGTATCCATTTGAAGG+TGG + chr7_4:29873415-29873434 Msa1149050:CDS 35.0%
GAATCTAAGTAACATCCCTA+GGG - chr7_4:29873852-29873871 None:intergenic 35.0%
AGAATCTAAGTAACATCCCT+AGG - chr7_4:29873853-29873872 None:intergenic 35.0%
GAAAGAAGAGAGGGATCAAA+GGG - chr7_4:29872303-29872322 None:intergenic 40.0%
AGAAAGAAGAGAGGGATCAA+AGG - chr7_4:29872304-29872323 None:intergenic 40.0%
! TGGGAGAGAAGTATAAACCA+TGG - chr7_4:29872490-29872509 None:intergenic 40.0%
TACTTCTCTCCCACCATATA+TGG + chr7_4:29872497-29872516 Msa1149050:CDS 40.0%
ATTTACCTGTTGATGCCAGT+TGG - chr7_4:29872529-29872548 None:intergenic 40.0%
GCAGCAACAAAATCATCAAG+TGG + chr7_4:29872703-29872722 Msa1149050:intron 40.0%
! CACCTTGGACATTTTTGTGA+TGG - chr7_4:29872875-29872894 None:intergenic 40.0%
GTAGCAAAACTTTGTGTTGG+TGG - chr7_4:29872903-29872922 None:intergenic 40.0%
!! TTGTCGAAGGTATTGGACAA+AGG + chr7_4:29872967-29872986 Msa1149050:CDS 40.0%
!! TGTCGAAGGTATTGGACAAA+GGG + chr7_4:29872968-29872987 Msa1149050:CDS 40.0%
! TGTTTGTAGAGCTAGTTTGG+CGG - chr7_4:29873093-29873112 None:intergenic 40.0%
AAAGGAGCCATGAATCTCAT+CGG - chr7_4:29873151-29873170 None:intergenic 40.0%
TGATCACCTAGATGATGCAA+AGG - chr7_4:29873169-29873188 None:intergenic 40.0%
GGTGATCACCTAAGTGAAAT+AGG + chr7_4:29873181-29873200 Msa1149050:CDS 40.0%
! CTATGGTTTTTCCAGTCCAA+TGG + chr7_4:29873210-29873229 Msa1149050:CDS 40.0%
TTCCATATCGGAAGCTCTTT+AGG + chr7_4:29873253-29873272 Msa1149050:CDS 40.0%
CATTGCTCAAAATTGCCAAC+AGG - chr7_4:29873331-29873350 None:intergenic 40.0%
! CTGTTGGCAATTTTGAGCAA+TGG + chr7_4:29873329-29873348 Msa1149050:CDS 40.0%
! TTGGCAATTTTGAGCAATGG+AGG + chr7_4:29873332-29873351 Msa1149050:CDS 40.0%
TTGTATCCATTTGAAGGTGG+TGG + chr7_4:29873418-29873437 Msa1149050:CDS 40.0%
AATGATCAATCCCATGCGTA+TGG + chr7_4:29873457-29873476 Msa1149050:CDS 40.0%
CAATATTGGAATGCTTCTGC+TGG + chr7_4:29873610-29873629 Msa1149050:CDS 40.0%
GAAAGAAGAGAGGGATCAAA+GGG - chr7_4:29872303-29872322 None:intergenic 40.0%
AGAAAGAAGAGAGGGATCAA+AGG - chr7_4:29872304-29872323 None:intergenic 40.0%
! TGGGAGAGAAGTATAAACCA+TGG - chr7_4:29872490-29872509 None:intergenic 40.0%
TACTTCTCTCCCACCATATA+TGG + chr7_4:29872497-29872516 Msa1149050:CDS 40.0%
ATTTACCTGTTGATGCCAGT+TGG - chr7_4:29872529-29872548 None:intergenic 40.0%
GCAGCAACAAAATCATCAAG+TGG + chr7_4:29872703-29872722 Msa1149050:intron 40.0%
! CACCTTGGACATTTTTGTGA+TGG - chr7_4:29872875-29872894 None:intergenic 40.0%
GTAGCAAAACTTTGTGTTGG+TGG - chr7_4:29872903-29872922 None:intergenic 40.0%
!! TTGTCGAAGGTATTGGACAA+AGG + chr7_4:29872967-29872986 Msa1149050:CDS 40.0%
!! TGTCGAAGGTATTGGACAAA+GGG + chr7_4:29872968-29872987 Msa1149050:CDS 40.0%
! TGTTTGTAGAGCTAGTTTGG+CGG - chr7_4:29873093-29873112 None:intergenic 40.0%
AAAGGAGCCATGAATCTCAT+CGG - chr7_4:29873151-29873170 None:intergenic 40.0%
TGATCACCTAGATGATGCAA+AGG - chr7_4:29873169-29873188 None:intergenic 40.0%
GGTGATCACCTAAGTGAAAT+AGG + chr7_4:29873181-29873200 Msa1149050:CDS 40.0%
! CTATGGTTTTTCCAGTCCAA+TGG + chr7_4:29873210-29873229 Msa1149050:CDS 40.0%
TTCCATATCGGAAGCTCTTT+AGG + chr7_4:29873253-29873272 Msa1149050:CDS 40.0%
CATTGCTCAAAATTGCCAAC+AGG - chr7_4:29873331-29873350 None:intergenic 40.0%
! CTGTTGGCAATTTTGAGCAA+TGG + chr7_4:29873329-29873348 Msa1149050:CDS 40.0%
! TTGGCAATTTTGAGCAATGG+AGG + chr7_4:29873332-29873351 Msa1149050:CDS 40.0%
TTGTATCCATTTGAAGGTGG+TGG + chr7_4:29873418-29873437 Msa1149050:CDS 40.0%
AATGATCAATCCCATGCGTA+TGG + chr7_4:29873457-29873476 Msa1149050:CDS 40.0%
CAATATTGGAATGCTTCTGC+TGG + chr7_4:29873610-29873629 Msa1149050:CDS 40.0%
AAAAGAGAGCTATCGGGGAA+TGG + chr7_4:29872171-29872190 Msa1149050:five_prime_UTR 45.0%
AAAGAGAGCTATCGGGGAAT+GGG + chr7_4:29872172-29872191 Msa1149050:five_prime_UTR 45.0%
AGAGAGGGATCAAAGGGTTA+AGG - chr7_4:29872297-29872316 None:intergenic 45.0%
AGCTCCAAGAGGAATAACCA+TGG + chr7_4:29872470-29872489 Msa1149050:exon 45.0%
TGGCTGGATCCATATATGGT+GGG - chr7_4:29872509-29872528 None:intergenic 45.0%
TTGGCTGGATCCATATATGG+TGG - chr7_4:29872510-29872529 None:intergenic 45.0%
CAGTTGGCTGGATCCATATA+TGG - chr7_4:29872513-29872532 None:intergenic 45.0%
CATATATGGATCCAGCCAAC+TGG + chr7_4:29872511-29872530 Msa1149050:CDS 45.0%
!! AGGAGTGTTGACACTAGTGT+TGG - chr7_4:29872729-29872748 None:intergenic 45.0%
!! TGTGGTGGTGGAATAAGTAG+AGG - chr7_4:29872749-29872768 None:intergenic 45.0%
GTGGTTGCCGAAGAAACAAA+AGG + chr7_4:29873020-29873039 Msa1149050:CDS 45.0%
CTCTACAAACAACTCTCCTG+CGG + chr7_4:29873102-29873121 Msa1149050:CDS 45.0%
ATGGCTCCTTTGCATCATCT+AGG + chr7_4:29873160-29873179 Msa1149050:CDS 45.0%
CACCTAAAGAGCTTCCGATA+TGG - chr7_4:29873258-29873277 None:intergenic 45.0%
CATATCGGAAGCTCTTTAGG+TGG + chr7_4:29873256-29873275 Msa1149050:CDS 45.0%
TATTGGAATGCTTCTGCTGG+TGG + chr7_4:29873613-29873632 Msa1149050:CDS 45.0%
!! GTGGTGCTACTAATTCACCT+TGG + chr7_4:29873635-29873654 Msa1149050:CDS 45.0%
GCTGTAGAAAGATCTGTCCA+AGG - chr7_4:29873655-29873674 None:intergenic 45.0%
AAAAGAGAGCTATCGGGGAA+TGG + chr7_4:29872171-29872190 Msa1149050:five_prime_UTR 45.0%
AAAGAGAGCTATCGGGGAAT+GGG + chr7_4:29872172-29872191 Msa1149050:five_prime_UTR 45.0%
AGAGAGGGATCAAAGGGTTA+AGG - chr7_4:29872297-29872316 None:intergenic 45.0%
AGCTCCAAGAGGAATAACCA+TGG + chr7_4:29872470-29872489 Msa1149050:exon 45.0%
TGGCTGGATCCATATATGGT+GGG - chr7_4:29872509-29872528 None:intergenic 45.0%
TTGGCTGGATCCATATATGG+TGG - chr7_4:29872510-29872529 None:intergenic 45.0%
CAGTTGGCTGGATCCATATA+TGG - chr7_4:29872513-29872532 None:intergenic 45.0%
CATATATGGATCCAGCCAAC+TGG + chr7_4:29872511-29872530 Msa1149050:CDS 45.0%
!! AGGAGTGTTGACACTAGTGT+TGG - chr7_4:29872729-29872748 None:intergenic 45.0%
!! TGTGGTGGTGGAATAAGTAG+AGG - chr7_4:29872749-29872768 None:intergenic 45.0%
GTGGTTGCCGAAGAAACAAA+AGG + chr7_4:29873020-29873039 Msa1149050:CDS 45.0%
CTCTACAAACAACTCTCCTG+CGG + chr7_4:29873102-29873121 Msa1149050:CDS 45.0%
ATGGCTCCTTTGCATCATCT+AGG + chr7_4:29873160-29873179 Msa1149050:CDS 45.0%
CACCTAAAGAGCTTCCGATA+TGG - chr7_4:29873258-29873277 None:intergenic 45.0%
CATATCGGAAGCTCTTTAGG+TGG + chr7_4:29873256-29873275 Msa1149050:CDS 45.0%
TATTGGAATGCTTCTGCTGG+TGG + chr7_4:29873613-29873632 Msa1149050:CDS 45.0%
!! GTGGTGCTACTAATTCACCT+TGG + chr7_4:29873635-29873654 Msa1149050:CDS 45.0%
GCTGTAGAAAGATCTGTCCA+AGG - chr7_4:29873655-29873674 None:intergenic 45.0%
GGATCAAAGGGTTAAGGAGG+TGG - chr7_4:29872291-29872310 None:intergenic 50.0%
GAGGGATCAAAGGGTTAAGG+AGG - chr7_4:29872294-29872313 None:intergenic 50.0%
ACCAGGTTCGATGGCAGATA+GGG + chr7_4:29872820-29872839 Msa1149050:CDS 50.0%
! GTGTTGGTGGAATCACACCT+TGG - chr7_4:29872890-29872909 None:intergenic 50.0%
TTCGACAAGCCTTGCAGAAG+TGG - chr7_4:29872955-29872974 None:intergenic 50.0%
ACTTCTGCAAGGCTTGTCGA+AGG + chr7_4:29872954-29872973 Msa1149050:CDS 50.0%
! GCAAGGCTTGTCGAAGGTAT+TGG + chr7_4:29872960-29872979 Msa1149050:CDS 50.0%
!! CGAAGGTATTGGACAAAGGG+CGG + chr7_4:29872971-29872990 Msa1149050:CDS 50.0%
TAACAACACCTCCAAGTCGC+CGG + chr7_4:29873057-29873076 Msa1149050:CDS 50.0%
! GGTTTTTCCAGTCCAATGGG+AGG + chr7_4:29873214-29873233 Msa1149050:CDS 50.0%
AAGTCTCCAACACCTCCCAT+TGG - chr7_4:29873229-29873248 None:intergenic 50.0%
ATCGGAAGCTCTTTAGGTGG+AGG + chr7_4:29873259-29873278 Msa1149050:CDS 50.0%
!! TTAGGTGGAGGCGTTGGTAA+CGG + chr7_4:29873271-29873290 Msa1149050:CDS 50.0%
!! GTTGGTAACGGTGGTGTCAA+CGG + chr7_4:29873283-29873302 Msa1149050:CDS 50.0%
! CCATTTGAAGGTGGTGGTAG+CGG + chr7_4:29873424-29873443 Msa1149050:CDS 50.0%
GATCAATCCCATGCGTATGG+TGG + chr7_4:29873460-29873479 Msa1149050:CDS 50.0%
AGACACACCACCATACGCAT+GGG - chr7_4:29873470-29873489 None:intergenic 50.0%
TAGACACACCACCATACGCA+TGG - chr7_4:29873471-29873490 None:intergenic 50.0%
! TGCGTATGGTGGTGTGTCTA+AGG + chr7_4:29873471-29873490 Msa1149050:CDS 50.0%
GGATCAAAGGGTTAAGGAGG+TGG - chr7_4:29872291-29872310 None:intergenic 50.0%
GAGGGATCAAAGGGTTAAGG+AGG - chr7_4:29872294-29872313 None:intergenic 50.0%
ACCAGGTTCGATGGCAGATA+GGG + chr7_4:29872820-29872839 Msa1149050:CDS 50.0%
! GTGTTGGTGGAATCACACCT+TGG - chr7_4:29872890-29872909 None:intergenic 50.0%
TTCGACAAGCCTTGCAGAAG+TGG - chr7_4:29872955-29872974 None:intergenic 50.0%
ACTTCTGCAAGGCTTGTCGA+AGG + chr7_4:29872954-29872973 Msa1149050:CDS 50.0%
! GCAAGGCTTGTCGAAGGTAT+TGG + chr7_4:29872960-29872979 Msa1149050:CDS 50.0%
!! CGAAGGTATTGGACAAAGGG+CGG + chr7_4:29872971-29872990 Msa1149050:CDS 50.0%
TAACAACACCTCCAAGTCGC+CGG + chr7_4:29873057-29873076 Msa1149050:CDS 50.0%
! GGTTTTTCCAGTCCAATGGG+AGG + chr7_4:29873214-29873233 Msa1149050:CDS 50.0%
AAGTCTCCAACACCTCCCAT+TGG - chr7_4:29873229-29873248 None:intergenic 50.0%
ATCGGAAGCTCTTTAGGTGG+AGG + chr7_4:29873259-29873278 Msa1149050:CDS 50.0%
!! TTAGGTGGAGGCGTTGGTAA+CGG + chr7_4:29873271-29873290 Msa1149050:CDS 50.0%
!! GTTGGTAACGGTGGTGTCAA+CGG + chr7_4:29873283-29873302 Msa1149050:CDS 50.0%
! CCATTTGAAGGTGGTGGTAG+CGG + chr7_4:29873424-29873443 Msa1149050:CDS 50.0%
GATCAATCCCATGCGTATGG+TGG + chr7_4:29873460-29873479 Msa1149050:CDS 50.0%
AGACACACCACCATACGCAT+GGG - chr7_4:29873470-29873489 None:intergenic 50.0%
TAGACACACCACCATACGCA+TGG - chr7_4:29873471-29873490 None:intergenic 50.0%
! TGCGTATGGTGGTGTGTCTA+AGG + chr7_4:29873471-29873490 Msa1149050:CDS 50.0%
AGAGCTATCGGGGAATGGGA+GGG + chr7_4:29872176-29872195 Msa1149050:five_prime_UTR 55.0%
TCCAGCCAACTGGCATCAAC+AGG + chr7_4:29872521-29872540 Msa1149050:CDS 55.0%
ACCTGTTGATGCCAGTTGGC+TGG - chr7_4:29872525-29872544 None:intergenic 55.0%
TTCGATGGTTGAGGCTGTGG+TGG - chr7_4:29872764-29872783 None:intergenic 55.0%
AGGTTCGATGGTTGAGGCTG+TGG - chr7_4:29872767-29872786 None:intergenic 55.0%
GGTGTCAGGTTCGATGGTTG+AGG - chr7_4:29872773-29872792 None:intergenic 55.0%
! TGCGATGGTGTCAGGTTCGA+TGG - chr7_4:29872779-29872798 None:intergenic 55.0%
CATGAGGTTGCGATGGTGTC+AGG - chr7_4:29872787-29872806 None:intergenic 55.0%
CTGACACCATCGCAACCTCA+TGG + chr7_4:29872785-29872804 Msa1149050:CDS 55.0%
ACACCATCGCAACCTCATGG+AGG + chr7_4:29872788-29872807 Msa1149050:CDS 55.0%
CTCGATCAGACCAGGTTCGA+TGG + chr7_4:29872811-29872830 Msa1149050:CDS 55.0%
GACCAGGTTCGATGGCAGAT+AGG + chr7_4:29872819-29872838 Msa1149050:CDS 55.0%
GTTCGATGGCAGATAGGGCA+AGG + chr7_4:29872825-29872844 Msa1149050:CDS 55.0%
GATGGCAGATAGGGCAAGGA+TGG + chr7_4:29872829-29872848 Msa1149050:CDS 55.0%
GCCAACCGGGACACTTCTAA+GGG - chr7_4:29872999-29873018 None:intergenic 55.0%
!! CTTAGAAGTGTCCCGGTTGG+CGG + chr7_4:29872998-29873017 Msa1149050:CDS 55.0%
! ATCTCATCGGTGGTGGCGTT+GGG - chr7_4:29873138-29873157 None:intergenic 55.0%
! AATCTCATCGGTGGTGGCGT+TGG - chr7_4:29873139-29873158 None:intergenic 55.0%
GCCACCACCGATGAGATTCA+TGG + chr7_4:29873141-29873160 Msa1149050:CDS 55.0%
GCCATGAATCTCATCGGTGG+TGG - chr7_4:29873145-29873164 None:intergenic 55.0%
GGAGCCATGAATCTCATCGG+TGG - chr7_4:29873148-29873167 None:intergenic 55.0%
! TCCAGTCCAATGGGAGGTGT+TGG + chr7_4:29873220-29873239 Msa1149050:CDS 55.0%
TCCAACACCTCCCATTGGAC+TGG - chr7_4:29873224-29873243 None:intergenic 55.0%
! AGCTCTTTAGGTGGAGGCGT+TGG + chr7_4:29873265-29873284 Msa1149050:CDS 55.0%
CCGCTACCACCACCTTCAAA+TGG - chr7_4:29873427-29873446 None:intergenic 55.0%
!! TTTGAAGGTGGTGGTAGCGG+TGG + chr7_4:29873427-29873446 Msa1149050:CDS 55.0%
TGGAATGCTTCTGCTGGTGG+TGG + chr7_4:29873616-29873635 Msa1149050:CDS 55.0%
AGAGCTATCGGGGAATGGGA+GGG + chr7_4:29872176-29872195 Msa1149050:five_prime_UTR 55.0%
TCCAGCCAACTGGCATCAAC+AGG + chr7_4:29872521-29872540 Msa1149050:CDS 55.0%
ACCTGTTGATGCCAGTTGGC+TGG - chr7_4:29872525-29872544 None:intergenic 55.0%
TTCGATGGTTGAGGCTGTGG+TGG - chr7_4:29872764-29872783 None:intergenic 55.0%
AGGTTCGATGGTTGAGGCTG+TGG - chr7_4:29872767-29872786 None:intergenic 55.0%
GGTGTCAGGTTCGATGGTTG+AGG - chr7_4:29872773-29872792 None:intergenic 55.0%
! TGCGATGGTGTCAGGTTCGA+TGG - chr7_4:29872779-29872798 None:intergenic 55.0%
CATGAGGTTGCGATGGTGTC+AGG - chr7_4:29872787-29872806 None:intergenic 55.0%
CTGACACCATCGCAACCTCA+TGG + chr7_4:29872785-29872804 Msa1149050:CDS 55.0%
ACACCATCGCAACCTCATGG+AGG + chr7_4:29872788-29872807 Msa1149050:CDS 55.0%
CTCGATCAGACCAGGTTCGA+TGG + chr7_4:29872811-29872830 Msa1149050:CDS 55.0%
GACCAGGTTCGATGGCAGAT+AGG + chr7_4:29872819-29872838 Msa1149050:CDS 55.0%
GTTCGATGGCAGATAGGGCA+AGG + chr7_4:29872825-29872844 Msa1149050:CDS 55.0%
GATGGCAGATAGGGCAAGGA+TGG + chr7_4:29872829-29872848 Msa1149050:CDS 55.0%
GCCAACCGGGACACTTCTAA+GGG - chr7_4:29872999-29873018 None:intergenic 55.0%
!! CTTAGAAGTGTCCCGGTTGG+CGG + chr7_4:29872998-29873017 Msa1149050:CDS 55.0%
! ATCTCATCGGTGGTGGCGTT+GGG - chr7_4:29873138-29873157 None:intergenic 55.0%
! AATCTCATCGGTGGTGGCGT+TGG - chr7_4:29873139-29873158 None:intergenic 55.0%
GCCACCACCGATGAGATTCA+TGG + chr7_4:29873141-29873160 Msa1149050:CDS 55.0%
GCCATGAATCTCATCGGTGG+TGG - chr7_4:29873145-29873164 None:intergenic 55.0%
GGAGCCATGAATCTCATCGG+TGG - chr7_4:29873148-29873167 None:intergenic 55.0%
! TCCAGTCCAATGGGAGGTGT+TGG + chr7_4:29873220-29873239 Msa1149050:CDS 55.0%
TCCAACACCTCCCATTGGAC+TGG - chr7_4:29873224-29873243 None:intergenic 55.0%
! AGCTCTTTAGGTGGAGGCGT+TGG + chr7_4:29873265-29873284 Msa1149050:CDS 55.0%
CCGCTACCACCACCTTCAAA+TGG - chr7_4:29873427-29873446 None:intergenic 55.0%
!! TTTGAAGGTGGTGGTAGCGG+TGG + chr7_4:29873427-29873446 Msa1149050:CDS 55.0%
TGGAATGCTTCTGCTGGTGG+TGG + chr7_4:29873616-29873635 Msa1149050:CDS 55.0%
GAGAGCTATCGGGGAATGGG+AGG + chr7_4:29872175-29872194 Msa1149050:five_prime_UTR 60.0%
GATGGTTGAGGCTGTGGTGG+TGG - chr7_4:29872761-29872780 None:intergenic 60.0%
GAGCCTCCATGAGGTTGCGA+TGG - chr7_4:29872794-29872813 None:intergenic 60.0%
GGTCTGATCGAGCCTCCATG+AGG - chr7_4:29872803-29872822 None:intergenic 60.0%
CATGGAGGCTCGATCAGACC+AGG + chr7_4:29872803-29872822 Msa1149050:CDS 60.0%
GCCCTATCTGCCATCGAACC+TGG - chr7_4:29872824-29872843 None:intergenic 60.0%
CAAGCCTTGCAGAAGTGGCG+TGG - chr7_4:29872950-29872969 None:intergenic 60.0%
!! CGGTGCCCTTAGAAGTGTCC+CGG + chr7_4:29872991-29873010 Msa1149050:CDS 60.0%
!! GCCCTTAGAAGTGTCCCGGT+TGG + chr7_4:29872995-29873014 Msa1149050:CDS 60.0%
CGCCAACCGGGACACTTCTA+AGG - chr7_4:29873000-29873019 None:intergenic 60.0%
GTTTGGCGGTCAGAGCTAGC+CGG - chr7_4:29873079-29873098 None:intergenic 60.0%
! GTTGGGGAACTGAGTACCGC+AGG - chr7_4:29873121-29873140 None:intergenic 60.0%
! TCTCATCGGTGGTGGCGTTG+GGG - chr7_4:29873137-29873156 None:intergenic 60.0%
! GGTAACGGTGGTGTCAACGG+TGG + chr7_4:29873286-29873305 Msa1149050:CDS 60.0%
! AACGGTGGTGTCAACGGTGG+TGG + chr7_4:29873289-29873308 Msa1149050:CDS 60.0%
GAGAGCTATCGGGGAATGGG+AGG + chr7_4:29872175-29872194 Msa1149050:five_prime_UTR 60.0%
GATGGTTGAGGCTGTGGTGG+TGG - chr7_4:29872761-29872780 None:intergenic 60.0%
GAGCCTCCATGAGGTTGCGA+TGG - chr7_4:29872794-29872813 None:intergenic 60.0%
GGTCTGATCGAGCCTCCATG+AGG - chr7_4:29872803-29872822 None:intergenic 60.0%
CATGGAGGCTCGATCAGACC+AGG + chr7_4:29872803-29872822 Msa1149050:CDS 60.0%
GCCCTATCTGCCATCGAACC+TGG - chr7_4:29872824-29872843 None:intergenic 60.0%
CAAGCCTTGCAGAAGTGGCG+TGG - chr7_4:29872950-29872969 None:intergenic 60.0%
!! CGGTGCCCTTAGAAGTGTCC+CGG + chr7_4:29872991-29873010 Msa1149050:CDS 60.0%
!! GCCCTTAGAAGTGTCCCGGT+TGG + chr7_4:29872995-29873014 Msa1149050:CDS 60.0%
CGCCAACCGGGACACTTCTA+AGG - chr7_4:29873000-29873019 None:intergenic 60.0%
GTTTGGCGGTCAGAGCTAGC+CGG - chr7_4:29873079-29873098 None:intergenic 60.0%
! GTTGGGGAACTGAGTACCGC+AGG - chr7_4:29873121-29873140 None:intergenic 60.0%
! TCTCATCGGTGGTGGCGTTG+GGG - chr7_4:29873137-29873156 None:intergenic 60.0%
! GGTAACGGTGGTGTCAACGG+TGG + chr7_4:29873286-29873305 Msa1149050:CDS 60.0%
! AACGGTGGTGTCAACGGTGG+TGG + chr7_4:29873289-29873308 Msa1149050:CDS 60.0%
GCAGCCACGCCACTTCTGCA+AGG + chr7_4:29872943-29872962 Msa1149050:CDS 65.0%
!! AGAAGTGTCCCGGTTGGCGG+TGG + chr7_4:29873001-29873020 Msa1149050:CDS 65.0%
TTCGGCAACCACCGCCAACC+GGG - chr7_4:29873012-29873031 None:intergenic 65.0%
CTTCGGCAACCACCGCCAAC+CGG - chr7_4:29873013-29873032 None:intergenic 65.0%
! AGAGCTAGCCGGCGACTTGG+AGG - chr7_4:29873068-29873087 None:intergenic 65.0%
GTCAGAGCTAGCCGGCGACT+TGG - chr7_4:29873071-29873090 None:intergenic 65.0%
!! GGTGGAGGCGTTGGTAACGG+TGG + chr7_4:29873274-29873293 Msa1149050:CDS 65.0%
!! GAAGGTGGTGGTAGCGGTGG+TGG + chr7_4:29873430-29873449 Msa1149050:CDS 65.0%
GCAGCCACGCCACTTCTGCA+AGG + chr7_4:29872943-29872962 Msa1149050:CDS 65.0%
!! AGAAGTGTCCCGGTTGGCGG+TGG + chr7_4:29873001-29873020 Msa1149050:CDS 65.0%
TTCGGCAACCACCGCCAACC+GGG - chr7_4:29873012-29873031 None:intergenic 65.0%
CTTCGGCAACCACCGCCAAC+CGG - chr7_4:29873013-29873032 None:intergenic 65.0%
! AGAGCTAGCCGGCGACTTGG+AGG - chr7_4:29873068-29873087 None:intergenic 65.0%
GTCAGAGCTAGCCGGCGACT+TGG - chr7_4:29873071-29873090 None:intergenic 65.0%
!! GGTGGAGGCGTTGGTAACGG+TGG + chr7_4:29873274-29873293 Msa1149050:CDS 65.0%
!! GAAGGTGGTGGTAGCGGTGG+TGG + chr7_4:29873430-29873449 Msa1149050:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 29872165 29873970 29872165 ID=Msa1149050;Name=Msa1149050
chr7_4 mRNA 29872165 29873970 29872165 ID=Msa1149050-mRNA-1;Parent=Msa1149050;Name=Msa1149050-mRNA-1;_AED=0.00;_eAED=0.00;_QI=324|1|1|1|1|1|2|250|355
chr7_4 exon 29872165 29872542 29872165 ID=Msa1149050-mRNA-1:exon:7322;Parent=Msa1149050-mRNA-1
chr7_4 exon 29872707 29873970 29872707 ID=Msa1149050-mRNA-1:exon:7323;Parent=Msa1149050-mRNA-1
chr7_4 five_prime_UTR 29872165 29872488 29872165 ID=Msa1149050-mRNA-1:five_prime_utr;Parent=Msa1149050-mRNA-1
chr7_4 CDS 29872489 29872542 29872489 ID=Msa1149050-mRNA-1:cds;Parent=Msa1149050-mRNA-1
chr7_4 CDS 29872707 29873720 29872707 ID=Msa1149050-mRNA-1:cds;Parent=Msa1149050-mRNA-1
chr7_4 three_prime_UTR 29873721 29873970 29873721 ID=Msa1149050-mRNA-1:three_prime_utr;Parent=Msa1149050-mRNA-1
Gene Sequence

>Msa1149050

ATGGTTTATACTTCTCTCCCACCATATATGGATCCAGCCAACTGGCATCAACAGCAACAAAATCATCAAGTGGCCAACACTAGTGTCAACACTCCTCTACTTATTCCACCACCACAGCCTCAACCATCGAACCTGACACCATCGCAACCTCATGGAGGCTCGATCAGACCAGGTTCGATGGCAGATAGGGCAAGGATGGCAAACATACCTATGCAAGAACCATCACAAAAATGTCCAAGGTGTGATTCCACCAACACAAAGTTTTGCTACTACAACAACTATAGTCTCTCGCAGCCACGCCACTTCTGCAAGGCTTGTCGAAGGTATTGGACAAAGGGCGGTGCCCTTAGAAGTGTCCCGGTTGGCGGTGGTTGCCGAAGAAACAAAAGGTCTAAATCAAGCAGTAACAACACCTCCAAGTCGCCGGCTAGCTCTGACCGCCAAACTAGCTCTACAAACAACTCTCCTGCGGTACTCAGTTCCCCAACGCCACCACCGATGAGATTCATGGCTCCTTTGCATCATCTAGGTGATCACCTAAGTGAAATAGGACTAAACTATGGTTTTTCCAGTCCAATGGGAGGTGTTGGAGACTTAAATTTCCATATCGGAAGCTCTTTAGGTGGAGGCGTTGGTAACGGTGGTGTCAACGGTGGTGGTTCTACTTCGATTTTACCTGTTGGCAATTTTGAGCAATGGAGGATGCCACAAACTCATCAATTTCCCTTCTTGTCAACTTTAGAAGCTTCTTCTTCACATGGATTGTTGTATCCATTTGAAGGTGGTGGTAGCGGTGGTGGTGCTAATGATCAATCCCATGCGTATGGTGGTGTGTCTAAGGTATTATCAAATAATTCTAGTGTGAAAATGGAAGAGAATCAATCTAGACAATTCTTGGGAATGATCAACAAAAACAACATCAATAATAATAATAATAATAATAATCCTAATAGTGAACAATATTGGAATGCTTCTGCTGGTGGTGGTGCTACTAATTCACCTTGGACAGATCTTTCTACAGCTTATAACACTTCTTCAGCTACTACAAATTCCAACTATAGGACATAA
Protein sequence

>Msa1149050

MVYTSLPPYMDPANWHQQQQNHQVANTSVNTPLLIPPPQPQPSNLTPSQPHGGSIRPGSMADRARMANIPMQEPSQKCPRCDSTNTKFCYYNNYSLSQPRHFCKACRRYWTKGGALRSVPVGGGCRRNKRSKSSSNNTSKSPASSDRQTSSTNNSPAVLSSPTPPPMRFMAPLHHLGDHLSEIGLNYGFSSPMGGVGDLNFHIGSSLGGGVGNGGVNGGGSTSILPVGNFEQWRMPQTHQFPFLSTLEASSSHGLLYPFEGGGSGGGANDQSHAYGGVSKVLSNNSSVKMEENQSRQFLGMINKNNINNNNNNNNPNSEQYWNASAGGGATNSPWTDLSTAYNTSSATTNSNYRT*