Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1149970 | AES97386.1 | 88.991 | 109 | 12 | 0 | 1 | 109 | 1 | 109 | 3.36e-65 | 202 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1149970 | G7JX45 | 88.991 | 109 | 12 | 0 | 1 | 109 | 1 | 109 | 1.61e-65 | 202 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1149970 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 130 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGACTTCAATGATTTCTTA+TGG | 0.226332 | 7_4:+32668247 | Msa1149970:CDS |
| AATATATACAAAACCTGATA+TGG | 0.287652 | 7_4:-32667645 | None:intergenic |
| AACTGTACATCTTCCATATC+AGG | 0.290482 | 7_4:+32667632 | Msa1149970:CDS |
| GAGACATTCTGTAACAATAA+TGG | 0.322182 | 7_4:+32668387 | Msa1149970:CDS |
| TGTACTCAAACGACAAGTTT+TGG | 0.337821 | 7_4:+32667599 | Msa1149970:CDS |
| AGACTTCAATGATTTCTTAT+GGG | 0.387413 | 7_4:+32668248 | Msa1149970:CDS |
| TTCTTTCTACTTCAAATGGC+TGG | 0.419263 | 7_4:+32667567 | None:intergenic |
| TATTCAGGGATTCATTGCTT+TGG | 0.427325 | 7_4:-32668344 | None:intergenic |
| AACGGATAGTGTCTCCAGTC+TGG | 0.449616 | 7_4:-32668413 | None:intergenic |
| CATAAGAAATCATTGAAGTC+TGG | 0.456714 | 7_4:-32668246 | None:intergenic |
| TCCCATCGAAGAACTTGAAA+CGG | 0.464329 | 7_4:-32668431 | None:intergenic |
| GTCCGTTTGTTCATGTCTAC+AGG | 0.467630 | 7_4:+32668460 | Msa1149970:CDS |
| ATCCGTTTCAAGTTCTTCGA+TGG | 0.488393 | 7_4:+32668429 | Msa1149970:CDS |
| TCCGTTTCAAGTTCTTCGAT+GGG | 0.508162 | 7_4:+32668430 | Msa1149970:CDS |
| AACAATAATGGATTCCAGAC+TGG | 0.518151 | 7_4:+32668399 | Msa1149970:CDS |
| GCTGTAACGAACTCATCATT+CGG | 0.528661 | 7_4:-32668309 | None:intergenic |
| TTCCTGTAGACATGAACAAA+CGG | 0.548025 | 7_4:-32668462 | None:intergenic |
| AAGAAATCATTGAAGTCTGG+TGG | 0.613745 | 7_4:-32668243 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATAATTTGGTTATAATAA+AGG | - | chr7_4:32668174-32668193 | None:intergenic | 15.0% |
| !! | ACATAATTTGGTTATAATAA+AGG | - | chr7_4:32668174-32668193 | None:intergenic | 15.0% |
| !! | AATATATACAAAACCTGATA+TGG | - | chr7_4:32667648-32667667 | None:intergenic | 20.0% |
| !!! | TCAAATTAAACAGAGTATTT+AGG | - | chr7_4:32667678-32667697 | None:intergenic | 20.0% |
| !! | TGTCAAAAAAAAATGATTTG+CGG | - | chr7_4:32668079-32668098 | None:intergenic | 20.0% |
| !! | AAAACATGTATTTGAGAATT+TGG | - | chr7_4:32668206-32668225 | None:intergenic | 20.0% |
| !!! | GTTTTTTAACTTAGTATTCA+GGG | - | chr7_4:32668361-32668380 | None:intergenic | 20.0% |
| !! | CTGAATACTAAGTTAAAAAA+CGG | + | chr7_4:32668360-32668379 | Msa1149970:CDS | 20.0% |
| !! | AATATATACAAAACCTGATA+TGG | - | chr7_4:32667648-32667667 | None:intergenic | 20.0% |
| !!! | TCAAATTAAACAGAGTATTT+AGG | - | chr7_4:32667678-32667697 | None:intergenic | 20.0% |
| !! | TGTCAAAAAAAAATGATTTG+CGG | - | chr7_4:32668079-32668098 | None:intergenic | 20.0% |
| !! | AAAACATGTATTTGAGAATT+TGG | - | chr7_4:32668206-32668225 | None:intergenic | 20.0% |
| !!! | GTTTTTTAACTTAGTATTCA+GGG | - | chr7_4:32668361-32668380 | None:intergenic | 20.0% |
| !! | CTGAATACTAAGTTAAAAAA+CGG | + | chr7_4:32668360-32668379 | Msa1149970:CDS | 20.0% |
| !! | TTAAACAGAGTATTTAGGAA+AGG | - | chr7_4:32667673-32667692 | None:intergenic | 25.0% |
| ! | AATGTAACTTTCAACAATTC+AGG | + | chr7_4:32667873-32667892 | Msa1149970:intron | 25.0% |
| !! | AAAAAAAATGATTTGCGGTT+CGG | - | chr7_4:32668074-32668093 | None:intergenic | 25.0% |
| ! | TGGTGAATAAGTACATAATT+TGG | - | chr7_4:32668186-32668205 | None:intergenic | 25.0% |
| !! | ATACATGTTTTGCAAATTAC+AGG | + | chr7_4:32668215-32668234 | Msa1149970:intron | 25.0% |
| ! | AGACTTCAATGATTTCTTAT+GGG | + | chr7_4:32668248-32668267 | Msa1149970:CDS | 25.0% |
| !!! | CGTTTTTTAACTTAGTATTC+AGG | - | chr7_4:32668362-32668381 | None:intergenic | 25.0% |
| ! | ATACTAAGTTAAAAAACGGT+TGG | + | chr7_4:32668364-32668383 | Msa1149970:CDS | 25.0% |
| ! | TACTAAGTTAAAAAACGGTT+GGG | + | chr7_4:32668365-32668384 | Msa1149970:CDS | 25.0% |
| !! | TTAAACAGAGTATTTAGGAA+AGG | - | chr7_4:32667673-32667692 | None:intergenic | 25.0% |
| ! | AATGTAACTTTCAACAATTC+AGG | + | chr7_4:32667873-32667892 | Msa1149970:intron | 25.0% |
| !! | AAAAAAAATGATTTGCGGTT+CGG | - | chr7_4:32668074-32668093 | None:intergenic | 25.0% |
| ! | TGGTGAATAAGTACATAATT+TGG | - | chr7_4:32668186-32668205 | None:intergenic | 25.0% |
| !! | ATACATGTTTTGCAAATTAC+AGG | + | chr7_4:32668215-32668234 | Msa1149970:intron | 25.0% |
| ! | AGACTTCAATGATTTCTTAT+GGG | + | chr7_4:32668248-32668267 | Msa1149970:CDS | 25.0% |
| !!! | CGTTTTTTAACTTAGTATTC+AGG | - | chr7_4:32668362-32668381 | None:intergenic | 25.0% |
| ! | ATACTAAGTTAAAAAACGGT+TGG | + | chr7_4:32668364-32668383 | Msa1149970:CDS | 25.0% |
| ! | TACTAAGTTAAAAAACGGTT+GGG | + | chr7_4:32668365-32668384 | Msa1149970:CDS | 25.0% |
| TCTGATTAAGTTAGACAACA+GGG | - | chr7_4:32667713-32667732 | None:intergenic | 30.0% | |
| ATCTGATTAAGTTAGACAAC+AGG | - | chr7_4:32667714-32667733 | None:intergenic | 30.0% | |
| CAAATGTTTATTATGTCCAC+AGG | + | chr7_4:32667761-32667780 | Msa1149970:intron | 30.0% | |
| TTATTATGTCCACAGGAATT+CGG | + | chr7_4:32667768-32667787 | Msa1149970:intron | 30.0% | |
| GGAATTCTTCAAGAAACTTT+GGG | - | chr7_4:32667803-32667822 | None:intergenic | 30.0% | |
| TGGAATTCTTCAAGAAACTT+TGG | - | chr7_4:32667804-32667823 | None:intergenic | 30.0% | |
| ! | CACTTTGACTAATTTGCATA+TGG | + | chr7_4:32668100-32668119 | Msa1149970:intron | 30.0% |
| CATAAGAAATCATTGAAGTC+TGG | - | chr7_4:32668249-32668268 | None:intergenic | 30.0% | |
| CAGACTTCAATGATTTCTTA+TGG | + | chr7_4:32668247-32668266 | Msa1149970:CDS | 30.0% | |
| ! | GATTTTCATTGCACAGAAAA+AGG | - | chr7_4:32668290-32668309 | None:intergenic | 30.0% |
| GAGACATTCTGTAACAATAA+TGG | + | chr7_4:32668387-32668406 | Msa1149970:CDS | 30.0% | |
| TCTGATTAAGTTAGACAACA+GGG | - | chr7_4:32667713-32667732 | None:intergenic | 30.0% | |
| ATCTGATTAAGTTAGACAAC+AGG | - | chr7_4:32667714-32667733 | None:intergenic | 30.0% | |
| CAAATGTTTATTATGTCCAC+AGG | + | chr7_4:32667761-32667780 | Msa1149970:intron | 30.0% | |
| TTATTATGTCCACAGGAATT+CGG | + | chr7_4:32667768-32667787 | Msa1149970:intron | 30.0% | |
| GGAATTCTTCAAGAAACTTT+GGG | - | chr7_4:32667803-32667822 | None:intergenic | 30.0% | |
| TGGAATTCTTCAAGAAACTT+TGG | - | chr7_4:32667804-32667823 | None:intergenic | 30.0% | |
| ! | CACTTTGACTAATTTGCATA+TGG | + | chr7_4:32668100-32668119 | Msa1149970:intron | 30.0% |
| CATAAGAAATCATTGAAGTC+TGG | - | chr7_4:32668249-32668268 | None:intergenic | 30.0% | |
| CAGACTTCAATGATTTCTTA+TGG | + | chr7_4:32668247-32668266 | Msa1149970:CDS | 30.0% | |
| ! | GATTTTCATTGCACAGAAAA+AGG | - | chr7_4:32668290-32668309 | None:intergenic | 30.0% |
| GAGACATTCTGTAACAATAA+TGG | + | chr7_4:32668387-32668406 | Msa1149970:CDS | 30.0% | |
| ! | TGTACTCAAACGACAAGTTT+TGG | + | chr7_4:32667599-32667618 | Msa1149970:CDS | 35.0% |
| ! | GTACTCAAACGACAAGTTTT+GGG | + | chr7_4:32667600-32667619 | Msa1149970:CDS | 35.0% |
| AACTGTACATCTTCCATATC+AGG | + | chr7_4:32667632-32667651 | Msa1149970:CDS | 35.0% | |
| ! | TCCATGATTTGAAACCAGTT+TGG | + | chr7_4:32667820-32667839 | Msa1149970:intron | 35.0% |
| ! | TCCAAACTGGTTTCAAATCA+TGG | - | chr7_4:32667824-32667843 | None:intergenic | 35.0% |
| ACATCTATCAGATTCCAAAC+TGG | - | chr7_4:32667837-32667856 | None:intergenic | 35.0% | |
| TGGAATCTGATAGATGTTGA+TGG | + | chr7_4:32667840-32667859 | Msa1149970:intron | 35.0% | |
| ! | ATCTATCCTTTACTGACTAC+AGG | + | chr7_4:32667897-32667916 | Msa1149970:intron | 35.0% |
| GAAATGGAAAGAATCTCTGA+TGG | - | chr7_4:32667931-32667950 | None:intergenic | 35.0% | |
| AGAGATTCTTTCCATTTCGA+AGG | + | chr7_4:32667933-32667952 | Msa1149970:intron | 35.0% | |
| TATGGCAGTTAGATCTGAAT+TGG | + | chr7_4:32668118-32668137 | Msa1149970:intron | 35.0% | |
| AAGAAATCATTGAAGTCTGG+TGG | - | chr7_4:32668246-32668265 | None:intergenic | 35.0% | |
| TATTCAGGGATTCATTGCTT+TGG | - | chr7_4:32668347-32668366 | None:intergenic | 35.0% | |
| !! | AACAATAATGGATTCCAGAC+TGG | + | chr7_4:32668399-32668418 | Msa1149970:CDS | 35.0% |
| TTCCTGTAGACATGAACAAA+CGG | - | chr7_4:32668465-32668484 | None:intergenic | 35.0% | |
| ! | TGTACTCAAACGACAAGTTT+TGG | + | chr7_4:32667599-32667618 | Msa1149970:CDS | 35.0% |
| ! | GTACTCAAACGACAAGTTTT+GGG | + | chr7_4:32667600-32667619 | Msa1149970:CDS | 35.0% |
| AACTGTACATCTTCCATATC+AGG | + | chr7_4:32667632-32667651 | Msa1149970:CDS | 35.0% | |
| ! | TCCATGATTTGAAACCAGTT+TGG | + | chr7_4:32667820-32667839 | Msa1149970:intron | 35.0% |
| ! | TCCAAACTGGTTTCAAATCA+TGG | - | chr7_4:32667824-32667843 | None:intergenic | 35.0% |
| ACATCTATCAGATTCCAAAC+TGG | - | chr7_4:32667837-32667856 | None:intergenic | 35.0% | |
| TGGAATCTGATAGATGTTGA+TGG | + | chr7_4:32667840-32667859 | Msa1149970:intron | 35.0% | |
| ! | ATCTATCCTTTACTGACTAC+AGG | + | chr7_4:32667897-32667916 | Msa1149970:intron | 35.0% |
| GAAATGGAAAGAATCTCTGA+TGG | - | chr7_4:32667931-32667950 | None:intergenic | 35.0% | |
| AGAGATTCTTTCCATTTCGA+AGG | + | chr7_4:32667933-32667952 | Msa1149970:intron | 35.0% | |
| TATGGCAGTTAGATCTGAAT+TGG | + | chr7_4:32668118-32668137 | Msa1149970:intron | 35.0% | |
| AAGAAATCATTGAAGTCTGG+TGG | - | chr7_4:32668246-32668265 | None:intergenic | 35.0% | |
| TATTCAGGGATTCATTGCTT+TGG | - | chr7_4:32668347-32668366 | None:intergenic | 35.0% | |
| !! | AACAATAATGGATTCCAGAC+TGG | + | chr7_4:32668399-32668418 | Msa1149970:CDS | 35.0% |
| TTCCTGTAGACATGAACAAA+CGG | - | chr7_4:32668465-32668484 | None:intergenic | 35.0% | |
| ! | ATCCTTTACTGACTACAGGA+TGG | + | chr7_4:32667901-32667920 | Msa1149970:intron | 40.0% |
| TTTGTCTCATGCCTTCGAAA+TGG | - | chr7_4:32667947-32667966 | None:intergenic | 40.0% | |
| CAAATCGTGCTACACTATGT+TGG | + | chr7_4:32667963-32667982 | Msa1149970:intron | 40.0% | |
| GCTGTAACGAACTCATCATT+CGG | - | chr7_4:32668312-32668331 | None:intergenic | 40.0% | |
| !! | ATCCGTTTCAAGTTCTTCGA+TGG | + | chr7_4:32668429-32668448 | Msa1149970:CDS | 40.0% |
| !! | TCCGTTTCAAGTTCTTCGAT+GGG | + | chr7_4:32668430-32668449 | Msa1149970:CDS | 40.0% |
| TCCCATCGAAGAACTTGAAA+CGG | - | chr7_4:32668434-32668453 | None:intergenic | 40.0% | |
| ! | ATCCTTTACTGACTACAGGA+TGG | + | chr7_4:32667901-32667920 | Msa1149970:intron | 40.0% |
| TTTGTCTCATGCCTTCGAAA+TGG | - | chr7_4:32667947-32667966 | None:intergenic | 40.0% | |
| CAAATCGTGCTACACTATGT+TGG | + | chr7_4:32667963-32667982 | Msa1149970:intron | 40.0% | |
| GCTGTAACGAACTCATCATT+CGG | - | chr7_4:32668312-32668331 | None:intergenic | 40.0% | |
| !! | ATCCGTTTCAAGTTCTTCGA+TGG | + | chr7_4:32668429-32668448 | Msa1149970:CDS | 40.0% |
| !! | TCCGTTTCAAGTTCTTCGAT+GGG | + | chr7_4:32668430-32668449 | Msa1149970:CDS | 40.0% |
| TCCCATCGAAGAACTTGAAA+CGG | - | chr7_4:32668434-32668453 | None:intergenic | 40.0% | |
| !!! | CTCAAACGACAAGTTTTGGG+AGG | + | chr7_4:32667603-32667622 | Msa1149970:CDS | 45.0% |
| GTCCATCCTGTAGTCAGTAA+AGG | - | chr7_4:32667906-32667925 | None:intergenic | 45.0% | |
| TTCCAAGAGCGAGGATGAAT+CGG | - | chr7_4:32667995-32668014 | None:intergenic | 45.0% | |
| TTTGTGCTTCTGCTGTGGAA+GGG | - | chr7_4:32668047-32668066 | None:intergenic | 45.0% | |
| GTCCGTTTGTTCATGTCTAC+AGG | + | chr7_4:32668460-32668479 | Msa1149970:CDS | 45.0% | |
| !!! | CTCAAACGACAAGTTTTGGG+AGG | + | chr7_4:32667603-32667622 | Msa1149970:CDS | 45.0% |
| GTCCATCCTGTAGTCAGTAA+AGG | - | chr7_4:32667906-32667925 | None:intergenic | 45.0% | |
| TTCCAAGAGCGAGGATGAAT+CGG | - | chr7_4:32667995-32668014 | None:intergenic | 45.0% | |
| TTTGTGCTTCTGCTGTGGAA+GGG | - | chr7_4:32668047-32668066 | None:intergenic | 45.0% | |
| GTCCGTTTGTTCATGTCTAC+AGG | + | chr7_4:32668460-32668479 | Msa1149970:CDS | 45.0% | |
| TTCACTTCGCCGAATTCCTG+TGG | - | chr7_4:32667780-32667799 | None:intergenic | 50.0% | |
| GATTCATCCTCGCTCTTGGA+AGG | + | chr7_4:32667994-32668013 | Msa1149970:intron | 50.0% | |
| GAAGGGAGGGAAATCATCAG+TGG | - | chr7_4:32668030-32668049 | None:intergenic | 50.0% | |
| CTTTGTGCTTCTGCTGTGGA+AGG | - | chr7_4:32668048-32668067 | None:intergenic | 50.0% | |
| !! | GTAGCTTTGTGCTTCTGCTG+TGG | - | chr7_4:32668052-32668071 | None:intergenic | 50.0% |
| AACGGATAGTGTCTCCAGTC+TGG | - | chr7_4:32668416-32668435 | None:intergenic | 50.0% | |
| TTCACTTCGCCGAATTCCTG+TGG | - | chr7_4:32667780-32667799 | None:intergenic | 50.0% | |
| GATTCATCCTCGCTCTTGGA+AGG | + | chr7_4:32667994-32668013 | Msa1149970:intron | 50.0% | |
| GAAGGGAGGGAAATCATCAG+TGG | - | chr7_4:32668030-32668049 | None:intergenic | 50.0% | |
| CTTTGTGCTTCTGCTGTGGA+AGG | - | chr7_4:32668048-32668067 | None:intergenic | 50.0% | |
| !! | GTAGCTTTGTGCTTCTGCTG+TGG | - | chr7_4:32668052-32668071 | None:intergenic | 50.0% |
| AACGGATAGTGTCTCCAGTC+TGG | - | chr7_4:32668416-32668435 | None:intergenic | 50.0% | |
| ! | GAGCGAGGATGAATCGGCTT+TGG | - | chr7_4:32667989-32668008 | None:intergenic | 55.0% |
| ! | AGCCGATTCATCCTCGCTCT+TGG | + | chr7_4:32667990-32668009 | Msa1149970:intron | 55.0% |
| ! | GAAGTGTCCTTCCAAGAGCG+AGG | - | chr7_4:32668004-32668023 | None:intergenic | 55.0% |
| TGCTTCTGCTGTGGAAGGGA+GGG | - | chr7_4:32668043-32668062 | None:intergenic | 55.0% | |
| ! | GAGCGAGGATGAATCGGCTT+TGG | - | chr7_4:32667989-32668008 | None:intergenic | 55.0% |
| ! | AGCCGATTCATCCTCGCTCT+TGG | + | chr7_4:32667990-32668009 | Msa1149970:intron | 55.0% |
| ! | GAAGTGTCCTTCCAAGAGCG+AGG | - | chr7_4:32668004-32668023 | None:intergenic | 55.0% |
| TGCTTCTGCTGTGGAAGGGA+GGG | - | chr7_4:32668043-32668062 | None:intergenic | 55.0% | |
| GTGCTTCTGCTGTGGAAGGG+AGG | - | chr7_4:32668044-32668063 | None:intergenic | 60.0% | |
| GTGCTTCTGCTGTGGAAGGG+AGG | - | chr7_4:32668044-32668063 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 32667582 | 32668494 | 32667582 | ID=Msa1149970;Name=Msa1149970 |
| chr7_4 | mRNA | 32667582 | 32668494 | 32667582 | ID=Msa1149970-mRNA-1;Parent=Msa1149970;Name=Msa1149970-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0|1|0|1|2|0|109 |
| chr7_4 | exon | 32667582 | 32667653 | 32667582 | ID=Msa1149970-mRNA-1:exon:7774;Parent=Msa1149970-mRNA-1 |
| chr7_4 | exon | 32668237 | 32668494 | 32668237 | ID=Msa1149970-mRNA-1:exon:7775;Parent=Msa1149970-mRNA-1 |
| chr7_4 | CDS | 32667582 | 32667653 | 32667582 | ID=Msa1149970-mRNA-1:cds;Parent=Msa1149970-mRNA-1 |
| chr7_4 | CDS | 32668237 | 32668494 | 32668237 | ID=Msa1149970-mRNA-1:cds;Parent=Msa1149970-mRNA-1 |
| Gene Sequence |
| Protein sequence |
