AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1157710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1157710 MtrunA17_Chr7g0250931 69.841 63 19 0 1 63 547 609 1.88e-24 94.7
Msa1157710 MtrunA17_Chr7g0238401 69.841 63 19 0 1 63 345 407 2.83e-24 94.4
Msa1157710 MtrunA17_Chr7g0251001 66.667 63 21 0 1 63 57 119 8.04e-24 92.0
Msa1157710 MtrunA17_Chr1g0212821 66.667 63 21 0 1 63 80 142 4.57e-23 90.1
Msa1157710 MtrunA17_Chr7g0251051 66.667 63 21 0 1 63 311 373 5.12e-23 90.9
Msa1157710 MtrunA17_Chr7g0250741 67.742 62 20 0 2 63 533 594 1.51e-22 89.4
Msa1157710 MtrunA17_Chr7g0250901 61.905 63 24 0 1 63 1 63 1.78e-22 84.3
Msa1157710 MtrunA17_Chr7g0251071 66.667 63 21 0 1 63 351 413 1.79e-22 89.4
Msa1157710 MtrunA17_Chr7g0251061 66.667 63 21 0 1 63 346 408 2.79e-22 88.6
Msa1157710 MtrunA17_Chr7g0232421 58.730 63 26 0 1 63 61 123 2.38e-21 85.1
Msa1157710 MtrunA17_Chr1g0209401 60.317 63 25 0 1 63 52 114 3.19e-21 84.7
Msa1157710 MtrunA17_Chr7g0251101 61.905 63 24 0 1 63 357 419 3.76e-21 85.5
Msa1157710 MtrunA17_Chr4g0022331 61.290 62 24 0 2 63 531 592 4.47e-21 85.1
Msa1157710 MtrunA17_Chr7g0251081 61.905 63 24 0 1 63 123 185 9.78e-21 84.0
Msa1157710 MtrunA17_Chr7g0232441 58.730 63 26 0 1 63 321 383 1.30e-20 84.0
Msa1157710 MtrunA17_Chr1g0209471 57.143 63 27 0 1 63 61 123 5.53e-20 81.6
Msa1157710 MtrunA17_Chr6g0483791 60.317 63 25 0 1 63 368 430 1.61e-19 80.9
Msa1157710 MtrunA17_Chr6g0483981 57.143 63 27 0 1 63 266 328 1.69e-19 80.9
Msa1157710 MtrunA17_Chr6g0483821 60.317 63 25 0 1 63 358 420 2.18e-19 80.5
Msa1157710 MtrunA17_Chr6g0484051 60.317 63 25 0 1 63 347 409 2.38e-19 80.1
Msa1157710 MtrunA17_Chr6g0484021 60.317 63 25 0 1 63 305 367 3.30e-19 79.7
Msa1157710 MtrunA17_Chr6g0484041 57.143 63 27 0 1 63 321 383 6.16e-19 79.0
Msa1157710 MtrunA17_Chr6g0483851 57.143 63 27 0 1 63 409 471 7.31e-19 79.0
Msa1157710 MtrunA17_Chr1g0209511 58.065 62 26 0 2 63 316 377 7.57e-19 79.0
Msa1157710 MtrunA17_Chr7g0253461 64.815 54 19 0 1 54 202 255 1.04e-18 77.0
Msa1157710 MtrunA17_Chr1g0209481 57.143 63 27 0 1 63 206 268 1.50e-18 77.8
Msa1157710 MtrunA17_Chr1g0209501 57.143 63 27 0 1 63 307 369 1.60e-18 77.8
Msa1157710 MtrunA17_Chr6g0484071 60.317 63 25 0 1 63 57 119 3.69e-18 76.6
Msa1157710 MtrunA17_Chr6g0483811 57.143 63 27 0 1 63 57 119 7.14e-18 75.9
Msa1157710 MtrunA17_Chr1g0209411 52.381 63 30 0 1 63 292 354 3.44e-17 73.9
Msa1157710 MtrunA17_Chr8g0391231 55.556 63 28 0 1 63 333 395 9.42e-17 72.8
Msa1157710 MtrunA17_Chr6g0484091 53.968 63 29 0 1 63 1 63 1.05e-16 68.2
Msa1157710 MtrunA17_Chr1g0158091 49.206 63 32 0 1 63 70 132 4.24e-16 70.9
Msa1157710 MtrunA17_Chr1g0158041 49.206 63 32 0 1 63 389 451 5.97e-16 70.5
Msa1157710 MtrunA17_Chr1g0158131 47.619 63 33 0 1 63 202 264 1.26e-15 69.7
Msa1157710 MtrunA17_Chr3g0088321 47.619 63 33 0 1 63 51 113 1.66e-15 69.3
Msa1157710 MtrunA17_Chr1g0157991 47.619 63 33 0 1 63 392 454 4.00e-15 68.2
Msa1157710 MtrunA17_Chr1g0157951 46.032 63 34 0 1 63 179 241 4.20e-15 68.2
Msa1157710 MtrunA17_Chr1g0160561 46.032 63 34 0 1 63 344 406 5.28e-15 67.8
Msa1157710 MtrunA17_Chr1g0158391 47.619 63 33 0 1 63 255 317 7.10e-15 67.4
Msa1157710 MtrunA17_Chr1g0160531 46.032 63 34 0 1 63 122 184 7.47e-15 67.4
Msa1157710 MtrunA17_Chr4g0037001 44.444 63 35 0 1 63 306 368 1.44e-14 66.6
Msa1157710 MtrunA17_Chr1g0160681 49.206 63 32 0 1 63 63 125 1.89e-14 66.2
Msa1157710 MtrunA17_Chr1g0157911 46.154 65 33 1 1 63 395 459 2.02e-14 66.2
Msa1157710 MtrunA17_Chr1g0157791 44.444 63 35 0 1 63 385 447 2.33e-14 66.2
Msa1157710 MtrunA17_Chr1g0208841 60.317 63 25 0 1 63 61 123 2.35e-14 65.9
Msa1157710 MtrunA17_Chr1g0158341 47.619 63 33 0 1 63 27 89 2.41e-14 65.9
Msa1157710 MtrunA17_Chr1g0157811 44.444 63 35 0 1 63 198 260 2.49e-14 65.9
Msa1157710 MtrunA17_Chr1g0157931 49.231 65 31 1 1 63 269 333 2.95e-14 65.9
Msa1157710 MtrunA17_Chr6g0467661 47.619 63 33 0 1 63 262 324 3.19e-14 65.9
Msa1157710 MtrunA17_Chr6g0467681 47.619 63 33 0 1 63 262 324 3.65e-14 65.5
Msa1157710 MtrunA17_Chr1g0158411 47.619 63 33 0 1 63 403 465 3.81e-14 65.5
Msa1157710 MtrunA17_Chr1g0158151 44.444 63 35 0 1 63 384 446 4.03e-14 65.5
Msa1157710 MtrunA17_Chr1g0158191 46.032 63 34 0 1 63 389 451 4.58e-14 65.1
Msa1157710 MtrunA17_Chr1g0157971 47.619 63 33 0 1 63 300 362 5.20e-14 65.1
Msa1157710 MtrunA17_Chr1g0158441 46.032 63 34 0 1 63 27 89 6.57e-14 64.7
Msa1157710 MtrunA17_Chr1g0157941 47.619 63 33 0 1 63 257 319 8.37e-14 64.7
Msa1157710 MtrunA17_Chr1g0158051 43.662 71 40 0 1 71 302 372 8.91e-14 64.3
Msa1157710 MtrunA17_Chr1g0157781 47.619 63 33 0 1 63 301 363 1.08e-13 64.3
Msa1157710 MtrunA17_Chr1g0158511 47.619 63 33 0 1 63 276 338 1.10e-13 64.3
Msa1157710 MtrunA17_Chr1g0160761 42.857 63 36 0 1 63 333 395 1.26e-13 63.9
Msa1157710 MtrunA17_Chr4g0036991 44.444 63 35 0 1 63 312 374 1.34e-13 63.9
Msa1157710 MtrunA17_Chr3g0088341 46.032 63 34 0 1 63 276 338 1.66e-13 63.5
Msa1157710 MtrunA17_Chr1g0160721 42.857 63 36 0 1 63 349 411 1.72e-13 63.5
Msa1157710 MtrunA17_Chr1g0160711 42.857 63 36 0 1 63 326 388 1.78e-13 63.5
Msa1157710 MtrunA17_Chr1g0157881 46.154 65 33 1 1 63 462 526 2.06e-13 63.5
Msa1157710 MtrunA17_Chr6g0467711 46.032 63 34 0 1 63 399 461 2.52e-13 63.2
Msa1157710 MtrunA17_Chr1g0160731 46.032 63 34 0 1 63 295 357 2.74e-13 63.2
Msa1157710 MtrunA17_Chr1g0160701 42.857 63 36 0 1 63 343 405 3.15e-13 62.8
Msa1157710 MtrunA17_Chr1g0160581 46.032 63 34 0 1 63 303 365 3.20e-13 62.8
Msa1157710 MtrunA17_Chr1g0158471 46.032 63 34 0 1 63 276 338 3.88e-13 62.8
Msa1157710 MtrunA17_Chr1g0158761 45.312 64 34 1 1 63 332 395 4.02e-13 62.8
Msa1157710 MtrunA17_Chr1g0157801 46.032 63 34 0 1 63 296 358 5.16e-13 62.4
Msa1157710 MtrunA17_Chr1g0157861 46.154 65 33 1 1 63 431 495 5.86e-13 62.0
Msa1157710 MtrunA17_Chr6g0467701 43.421 76 41 1 1 74 32 107 7.71e-13 61.6
Msa1157710 MtrunA17_Chr1g0160771 44.444 63 35 0 1 63 299 361 7.85e-13 61.6
Msa1157710 MtrunA17_Chr1g0158651 42.857 63 36 0 1 63 305 367 8.91e-13 61.6
Msa1157710 MtrunA17_Chr6g0467641 47.619 63 33 0 1 63 191 253 1.11e-12 61.2
Msa1157710 MtrunA17_Chr1g0158101 44.444 63 35 0 1 63 306 368 1.26e-12 61.2
Msa1157710 MtrunA17_Chr1g0158301 46.875 64 33 1 1 63 391 454 1.30e-12 61.2
Msa1157710 MtrunA17_Chr1g0158491 45.763 59 32 0 1 59 100 158 2.24e-12 58.5
Msa1157710 MtrunA17_Chr1g0158611 42.857 63 36 0 1 63 49 111 2.31e-12 60.5
Msa1157710 MtrunA17_Chr1g0158321 43.421 76 41 1 1 74 32 107 3.50e-12 59.7
Msa1157710 MtrunA17_Chr1g0158711 46.032 63 34 0 1 63 259 321 3.80e-12 59.7
Msa1157710 MtrunA17_Chr1g0160661 44.444 63 35 0 1 63 350 412 4.15e-12 59.7
Msa1157710 MtrunA17_Chr1g0206291 47.692 65 31 2 2 63 351 415 4.65e-12 59.7
Msa1157710 MtrunA17_Chr7g0236121 43.038 79 35 3 2 77 502 573 4.79e-12 59.7
Msa1157710 MtrunA17_Chr7g0236161 43.038 79 35 3 2 77 468 539 5.06e-12 59.3
Msa1157710 MtrunA17_Chr1g0157871 46.032 63 34 0 1 63 259 321 5.15e-12 59.3
Msa1157710 MtrunA17_Chr1g0206231 43.750 80 36 3 2 78 344 417 5.29e-12 59.3
Msa1157710 MtrunA17_Chr7g0236201 43.038 79 35 3 2 77 468 539 6.73e-12 59.3
Msa1157710 MtrunA17_Chr1g0158311 46.032 63 34 0 1 63 286 348 7.48e-12 58.9
Msa1157710 MtrunA17_Chr1g0158771 42.857 63 36 0 1 63 287 349 8.00e-12 58.9
Msa1157710 MtrunA17_Chr1g0158541 45.312 64 34 1 1 63 393 456 8.31e-12 58.9
Msa1157710 MtrunA17_Chr1g0206351 46.154 65 32 2 2 63 351 415 1.92e-11 57.8
Msa1157710 MtrunA17_Chr7g0263591 41.270 63 36 1 2 63 603 665 2.51e-11 57.4
Msa1157710 MtrunA17_Chr8g0340111 45.000 80 35 3 2 78 502 575 2.54e-11 57.4
Msa1157710 MtrunA17_Chr8g0340091 41.333 75 37 2 5 78 534 602 2.70e-11 57.4
Msa1157710 MtrunA17_Chr1g0158571 43.750 64 35 1 1 63 210 273 2.91e-11 57.4
Msa1157710 MtrunA17_Chr3g0123141 39.683 63 37 1 2 63 607 669 3.29e-11 57.0
Msa1157710 MtrunA17_Chr8g0354421 49.231 65 30 2 2 63 361 425 3.45e-11 57.0
Msa1157710 MtrunA17_Chr1g0158531 44.444 63 35 0 1 63 288 350 4.09e-11 57.0
Msa1157710 MtrunA17_Chr1g0181731 44.444 63 34 1 2 63 597 659 4.29e-11 57.0
Msa1157710 MtrunA17_Chr8g0354271 49.231 65 30 2 2 63 333 397 4.75e-11 56.6
Msa1157710 MtrunA17_Chr1g0158751 43.750 64 35 1 1 63 93 156 4.95e-11 56.6
Msa1157710 MtrunA17_Chr1g0158181 39.130 69 42 0 1 69 324 392 5.83e-11 56.6
Msa1157710 MtrunA17_Chr6g0484031 57.447 47 20 0 1 47 221 267 6.10e-11 56.2
Msa1157710 MtrunA17_Chr1g0206421 49.153 59 29 1 6 63 232 290 7.10e-11 56.2
Msa1157710 MtrunA17_Chr7g0236471 46.667 60 31 1 5 63 343 402 7.53e-11 56.2
Msa1157710 MtrunA17_Chr8g0340051 42.500 80 37 3 2 78 26 99 8.02e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1157710 AT1G66910.1 57.143 63 27 0 1 63 346 408 7.47e-20 82.0
Msa1157710 AT5G38250.1 58.730 63 26 0 1 63 312 374 7.52e-20 82.0
Msa1157710 AT1G66910.2 57.143 63 27 0 1 63 343 405 9.53e-20 81.6
Msa1157710 AT1G66980.2 57.143 63 27 0 1 63 832 894 1.05e-19 81.6
Msa1157710 AT1G66980.4 57.143 63 27 0 1 63 786 848 1.20e-19 81.6
Msa1157710 AT1G66980.3 57.143 63 27 0 1 63 830 892 1.20e-19 81.6
Msa1157710 AT1G66980.1 57.143 63 27 0 1 63 803 865 1.23e-19 81.6
Msa1157710 AT5G38240.2 61.905 63 24 0 1 63 315 377 2.05e-19 80.9
Msa1157710 AT5G38240.1 61.905 63 24 0 1 63 333 395 2.05e-19 80.9
Msa1157710 AT5G38240.3 61.905 63 24 0 1 63 269 331 2.32e-19 80.9
Msa1157710 AT5G38260.1 60.317 63 25 0 1 63 319 381 4.70e-19 79.7
Msa1157710 AT1G66920.1 57.812 64 26 1 1 63 297 360 1.03e-18 79.0
Msa1157710 AT1G66920.2 57.812 64 26 1 1 63 305 368 1.03e-18 79.0
Msa1157710 AT1G67000.1 59.375 64 25 1 1 63 554 617 1.11e-18 79.0
Msa1157710 AT5G38280.2 62.500 64 23 1 1 63 329 392 1.18e-18 78.6
Msa1157710 AT5G38280.1 62.500 64 23 1 1 63 329 392 1.99e-18 78.2
Msa1157710 AT1G70250.1 59.375 64 25 1 1 63 457 520 3.08e-18 77.4
Msa1157710 AT1G66930.2 57.812 64 26 1 1 63 553 616 3.98e-18 77.4
Msa1157710 AT1G66930.1 57.812 64 26 1 1 63 344 407 7.87e-18 76.3
Msa1157710 AT5G39030.1 55.556 63 28 0 1 63 496 558 8.82e-18 76.3
Msa1157710 AT5G39030.2 55.556 63 28 0 1 63 490 552 1.22e-17 75.9
Msa1157710 AT4G18250.1 53.846 65 28 1 1 63 353 417 1.54e-17 75.5
Msa1157710 AT5G39020.1 57.143 63 27 0 1 63 494 556 2.10e-17 75.1
Msa1157710 AT1G36005.1 53.968 63 29 0 1 63 16 78 4.22e-17 71.6
Msa1157710 AT1G61550.1 47.692 65 31 2 2 63 486 550 9.38e-13 62.0
Msa1157710 AT1G61370.2 45.455 66 33 2 1 63 346 411 2.01e-12 60.8
Msa1157710 AT1G61430.9 49.231 65 30 2 2 63 486 550 2.35e-12 60.8
Msa1157710 AT1G61370.1 45.455 66 33 2 1 63 497 562 2.37e-12 60.8
Msa1157710 AT1G61500.3 47.692 65 31 2 2 63 493 557 2.44e-12 60.8
Msa1157710 AT1G61500.1 47.692 65 31 2 2 63 488 552 2.46e-12 60.8
Msa1157710 AT1G61420.3 47.692 65 31 2 2 63 341 405 2.47e-12 60.8
Msa1157710 AT1G61420.5 47.692 65 31 2 2 63 343 407 2.49e-12 60.8
Msa1157710 AT1G61430.11 49.231 65 30 2 2 63 463 527 2.58e-12 60.8
Msa1157710 AT1G61430.12 49.231 65 30 2 2 63 463 527 2.58e-12 60.8
Msa1157710 AT1G61430.13 49.231 65 30 2 2 63 463 527 2.58e-12 60.8
Msa1157710 AT1G61490.3 47.692 65 31 2 2 63 337 401 2.86e-12 60.5
Msa1157710 AT1G61460.1 47.692 65 31 2 2 63 451 515 2.93e-12 60.5
Msa1157710 AT4G20450.1 43.750 64 35 1 1 63 589 652 2.98e-12 60.5
Msa1157710 AT4G20450.2 43.750 64 35 1 1 63 589 652 3.13e-12 60.5
Msa1157710 AT1G61500.2 47.692 65 31 2 2 63 470 534 3.24e-12 60.5
Msa1157710 AT1G61430.8 49.231 65 30 2 2 63 486 550 3.45e-12 60.5
Msa1157710 AT1G61430.7 49.231 65 30 2 2 63 486 550 3.45e-12 60.5
Msa1157710 AT1G61430.2 49.231 65 30 2 2 63 486 550 3.59e-12 60.5
Msa1157710 AT1G61430.6 49.231 65 30 2 2 63 486 550 3.59e-12 60.5
Msa1157710 AT1G61490.6 47.692 65 31 2 2 63 461 525 3.93e-12 60.1
Msa1157710 AT1G61490.7 47.692 65 31 2 2 63 461 525 3.93e-12 60.1
Msa1157710 AT1G61430.10 49.231 65 30 2 2 63 463 527 4.15e-12 60.1
Msa1157710 AT1G61490.4 47.692 65 31 2 2 63 487 551 4.38e-12 60.1
Msa1157710 AT1G61490.1 47.692 65 31 2 2 63 487 551 4.38e-12 60.1
Msa1157710 AT1G61490.2 47.692 65 31 2 2 63 487 551 4.38e-12 60.1
Msa1157710 AT1G61490.5 47.692 65 31 2 2 63 487 551 4.38e-12 60.1
Msa1157710 AT1G61420.1 47.692 65 31 2 2 63 491 555 4.60e-12 60.1
Msa1157710 AT1G61420.2 47.692 65 31 2 2 63 493 557 4.60e-12 60.1
Msa1157710 AT1G61420.4 47.692 65 31 2 2 63 493 557 4.60e-12 60.1
Msa1157710 AT1G61430.4 49.231 65 30 2 2 63 463 527 4.62e-12 60.1
Msa1157710 AT1G61430.3 49.231 65 30 2 2 63 495 559 6.03e-12 59.7
Msa1157710 AT5G16900.1 45.312 64 34 1 1 63 571 634 6.82e-12 59.7
Msa1157710 AT5G16900.3 45.312 64 34 1 1 63 571 634 6.82e-12 59.7
Msa1157710 AT1G61390.2 47.692 65 31 2 2 63 349 413 7.15e-12 59.3
Msa1157710 AT1G61390.1 47.692 65 31 2 2 63 517 581 7.22e-12 59.3
Msa1157710 AT1G61480.1 45.902 61 32 1 4 63 497 557 8.86e-12 59.3
Msa1157710 AT5G16900.4 45.312 64 34 1 1 63 385 448 9.23e-12 59.3
Msa1157710 AT4G35030.8 44.286 70 32 2 6 74 107 170 1.35e-11 58.5
Msa1157710 AT4G35030.1 44.286 70 32 2 6 74 107 170 1.35e-11 58.5
Msa1157710 AT4G35030.2 44.286 70 32 2 6 74 107 170 2.55e-11 57.8
Msa1157710 AT1G61430.1 48.529 68 29 3 2 63 486 553 2.70e-11 57.8
Msa1157710 AT4G35030.4 44.286 70 32 2 6 74 107 170 2.71e-11 57.8
Msa1157710 AT3G46400.1 43.750 64 35 1 1 63 574 637 3.16e-11 57.8
Msa1157710 AT1G61400.2 47.692 65 31 2 2 63 497 561 3.25e-11 57.8
Msa1157710 AT1G11280.2 41.772 79 36 3 2 77 502 573 3.72e-11 57.4
Msa1157710 AT1G61400.1 47.692 65 31 2 2 63 497 561 3.83e-11 57.4
Msa1157710 AT1G11280.3 41.772 79 36 3 2 77 490 561 3.91e-11 57.4
Msa1157710 AT1G61430.5 48.529 68 29 3 2 63 486 553 3.97e-11 57.4
Msa1157710 AT1G11280.1 41.772 79 36 3 2 77 512 583 4.07e-11 57.4
Msa1157710 AT1G11280.4 41.772 79 36 3 2 77 500 571 4.11e-11 57.4
Msa1157710 AT4G35030.7 44.286 70 32 2 6 74 107 170 4.38e-11 57.0
Msa1157710 AT4G35030.3 44.286 70 32 2 6 74 107 170 4.38e-11 57.0
Msa1157710 AT4G35030.6 44.286 70 32 2 6 74 107 170 4.38e-11 57.0
Msa1157710 AT4G35030.5 44.286 70 32 2 6 74 107 170 4.38e-11 57.0
Msa1157710 AT2G04300.2 45.312 64 34 1 1 63 580 643 5.11e-11 57.0
Msa1157710 AT1G11280.5 41.772 79 36 3 2 77 502 573 5.67e-11 57.0
Msa1157710 AT1G61440.3 45.902 61 32 1 4 63 484 544 5.72e-11 57.0
Msa1157710 AT1G61440.1 45.902 61 32 1 4 63 479 539 5.77e-11 57.0
Msa1157710 AT1G61380.2 46.154 65 32 2 2 63 338 402 5.78e-11 57.0
Msa1157710 AT1G61380.1 46.154 65 32 2 2 63 485 549 6.49e-11 56.6
Msa1157710 AT2G04300.1 45.312 64 34 1 1 63 539 602 7.04e-11 56.6
Msa1157710 AT2G16750.2 43.662 71 33 2 4 73 278 342 9.12e-11 56.2
Msa1157710 AT2G16750.1 43.662 71 33 2 4 73 278 342 9.12e-11 56.2

Find 15 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGAACATCGCTTCACTTT+TGG 0.209908 7_4:+46789881 Msa1157710:CDS
AATACCTTTGAAGAGAAATT+AGG 0.274194 7_4:+46789723 Msa1157710:CDS
AAATTAGGTGAAGGAGGATT+TGG 0.351930 7_4:+46790449 Msa1157710:intron
TTTACCTAATTTCTCTTCAA+AGG 0.460863 7_4:-46789727 None:intergenic
GAAGAGAAATTAGGTAAAGG+AGG 0.467581 7_4:+46789732 Msa1157710:CDS
GAAAATAATAAATGATTCAA+AGG 0.476405 7_4:+46789806 Msa1157710:CDS
CGAGATAAATTAGGTGAAGG+AGG 0.506838 7_4:+46790443 Msa1157710:intron
ATAAATGATTCAAAGGGAGA+TGG 0.530038 7_4:+46789813 Msa1157710:CDS
TTTGAAGAGAAATTAGGTAA+AGG 0.533222 7_4:+46789729 Msa1157710:CDS
GGTGTTGTGTGCTTGCATGA+TGG 0.533478 7_4:+46790470 Msa1157710:CDS
AAAATAATAAATGATTCAAA+GGG 0.544205 7_4:+46789807 Msa1157710:CDS
ACGTGATGGTCGTCAAGTAG+CGG 0.612643 7_4:+46789782 Msa1157710:CDS
TGAAGCGATGTTCACATGTG+AGG 0.622079 7_4:-46789874 None:intergenic
TACAATTCAAGCTTACGTGA+TGG 0.643167 7_4:+46789768 Msa1157710:CDS
TGCATGATGGTCGTCAAGTG+CGG 0.682103 7_4:+46790483 Msa1157710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATAAATGATTCAAA+GGG + chr7_4:46789807-46789826 Msa1157710:CDS 10.0%
!!! TATTTTTAATCTTATTTAGT+TGG + chr7_4:46790231-46790250 Msa1157710:intron 10.0%
!! AAAATAATAAATGATTCAAA+GGG + chr7_4:46789807-46789826 Msa1157710:CDS 10.0%
!!! TATTTTTAATCTTATTTAGT+TGG + chr7_4:46790231-46790250 Msa1157710:intron 10.0%
!! GAAAATAATAAATGATTCAA+AGG + chr7_4:46789806-46789825 Msa1157710:CDS 15.0%
!! GAAAATAATAAATGATTCAA+AGG + chr7_4:46789806-46789825 Msa1157710:CDS 15.0%
!! CATAGATTAACATAAAAATG+AGG - chr7_4:46790020-46790039 None:intergenic 20.0%
!!! CTCATTTTTATGTTAATCTA+TGG + chr7_4:46790018-46790037 Msa1157710:intron 20.0%
!!! TAGTTTATGTTTTGTAAGTT+AGG + chr7_4:46790135-46790154 Msa1157710:intron 20.0%
!! CATAGATTAACATAAAAATG+AGG - chr7_4:46790020-46790039 None:intergenic 20.0%
!!! CTCATTTTTATGTTAATCTA+TGG + chr7_4:46790018-46790037 Msa1157710:intron 20.0%
!!! TAGTTTATGTTTTGTAAGTT+AGG + chr7_4:46790135-46790154 Msa1157710:intron 20.0%
!! AATACCTTTGAAGAGAAATT+AGG + chr7_4:46789723-46789742 Msa1157710:CDS 25.0%
! TTTACCTAATTTCTCTTCAA+AGG - chr7_4:46789730-46789749 None:intergenic 25.0%
! TTTGAAGAGAAATTAGGTAA+AGG + chr7_4:46789729-46789748 Msa1157710:CDS 25.0%
!! TTTTAATCTTTGCTTAGCAT+TGG + chr7_4:46790273-46790292 Msa1157710:intron 25.0%
! CGTATACAAATAATCACAAT+AGG - chr7_4:46790309-46790328 None:intergenic 25.0%
! TGATTATTTGTATACGTAAG+AGG + chr7_4:46790313-46790332 Msa1157710:intron 25.0%
!! AACTTGTTTCGAGATAAATT+AGG + chr7_4:46790434-46790453 Msa1157710:intron 25.0%
!! AATACCTTTGAAGAGAAATT+AGG + chr7_4:46789723-46789742 Msa1157710:CDS 25.0%
! TTTACCTAATTTCTCTTCAA+AGG - chr7_4:46789730-46789749 None:intergenic 25.0%
! TTTGAAGAGAAATTAGGTAA+AGG + chr7_4:46789729-46789748 Msa1157710:CDS 25.0%
!! TTTTAATCTTTGCTTAGCAT+TGG + chr7_4:46790273-46790292 Msa1157710:intron 25.0%
! CGTATACAAATAATCACAAT+AGG - chr7_4:46790309-46790328 None:intergenic 25.0%
! TGATTATTTGTATACGTAAG+AGG + chr7_4:46790313-46790332 Msa1157710:intron 25.0%
!! AACTTGTTTCGAGATAAATT+AGG + chr7_4:46790434-46790453 Msa1157710:intron 25.0%
ATAAATGATTCAAAGGGAGA+TGG + chr7_4:46789813-46789832 Msa1157710:CDS 30.0%
TTCACTAATAGACCTTCTAA+GGG + chr7_4:46789980-46789999 Msa1157710:intron 30.0%
! TATGTTTTGTAAGTTAGGAC+TGG + chr7_4:46790140-46790159 Msa1157710:intron 30.0%
!! TCATCTATTTGTTAGTCTTG+TGG + chr7_4:46790164-46790183 Msa1157710:intron 30.0%
TTTCGAGATAAATTAGGTGA+AGG + chr7_4:46790440-46790459 Msa1157710:intron 30.0%
ATAAATGATTCAAAGGGAGA+TGG + chr7_4:46789813-46789832 Msa1157710:CDS 30.0%
TTCACTAATAGACCTTCTAA+GGG + chr7_4:46789980-46789999 Msa1157710:intron 30.0%
! TATGTTTTGTAAGTTAGGAC+TGG + chr7_4:46790140-46790159 Msa1157710:intron 30.0%
!! TCATCTATTTGTTAGTCTTG+TGG + chr7_4:46790164-46790183 Msa1157710:intron 30.0%
TTTCGAGATAAATTAGGTGA+AGG + chr7_4:46790440-46790459 Msa1157710:intron 30.0%
GAAGAGAAATTAGGTAAAGG+AGG + chr7_4:46789732-46789751 Msa1157710:CDS 35.0%
TACAATTCAAGCTTACGTGA+TGG + chr7_4:46789768-46789787 Msa1157710:CDS 35.0%
ATCTATGACTTCATGTCCAA+AGG + chr7_4:46789938-46789957 Msa1157710:intron 35.0%
GAACTTATCCAATGATCCTT+TGG - chr7_4:46789957-46789976 None:intergenic 35.0%
CTTCACTAATAGACCTTCTA+AGG + chr7_4:46789979-46789998 Msa1157710:intron 35.0%
!! TTGTTAGTCTTGTGGTTAGT+TGG + chr7_4:46790172-46790191 Msa1157710:intron 35.0%
AAATTAGGTGAAGGAGGATT+TGG + chr7_4:46790449-46790468 Msa1157710:intron 35.0%
GAAGAGAAATTAGGTAAAGG+AGG + chr7_4:46789732-46789751 Msa1157710:CDS 35.0%
TACAATTCAAGCTTACGTGA+TGG + chr7_4:46789768-46789787 Msa1157710:CDS 35.0%
ATCTATGACTTCATGTCCAA+AGG + chr7_4:46789938-46789957 Msa1157710:intron 35.0%
GAACTTATCCAATGATCCTT+TGG - chr7_4:46789957-46789976 None:intergenic 35.0%
CTTCACTAATAGACCTTCTA+AGG + chr7_4:46789979-46789998 Msa1157710:intron 35.0%
!! TTGTTAGTCTTGTGGTTAGT+TGG + chr7_4:46790172-46790191 Msa1157710:intron 35.0%
AAATTAGGTGAAGGAGGATT+TGG + chr7_4:46790449-46790468 Msa1157710:intron 35.0%
! TGTGAACATCGCTTCACTTT+TGG + chr7_4:46789881-46789900 Msa1157710:CDS 40.0%
!! GTGAACATCGCTTCACTTTT+GGG + chr7_4:46789882-46789901 Msa1157710:CDS 40.0%
CTTCATGTCCAAAGGATCAT+TGG + chr7_4:46789946-46789965 Msa1157710:intron 40.0%
TTGACACAGAAACCCTTAGA+AGG - chr7_4:46789995-46790014 None:intergenic 40.0%
CGAGATAAATTAGGTGAAGG+AGG + chr7_4:46790443-46790462 Msa1157710:intron 40.0%
! TGTGAACATCGCTTCACTTT+TGG + chr7_4:46789881-46789900 Msa1157710:CDS 40.0%
!! GTGAACATCGCTTCACTTTT+GGG + chr7_4:46789882-46789901 Msa1157710:CDS 40.0%
CTTCATGTCCAAAGGATCAT+TGG + chr7_4:46789946-46789965 Msa1157710:intron 40.0%
TTGACACAGAAACCCTTAGA+AGG - chr7_4:46789995-46790014 None:intergenic 40.0%
CGAGATAAATTAGGTGAAGG+AGG + chr7_4:46790443-46790462 Msa1157710:intron 40.0%
TGAAGCGATGTTCACATGTG+AGG - chr7_4:46789877-46789896 None:intergenic 45.0%
TGAAGCGATGTTCACATGTG+AGG - chr7_4:46789877-46789896 None:intergenic 45.0%
ACGTGATGGTCGTCAAGTAG+CGG + chr7_4:46789782-46789801 Msa1157710:CDS 50.0%
! GGTGTTGTGTGCTTGCATGA+TGG + chr7_4:46790470-46790489 Msa1157710:CDS 50.0%
TGCATGATGGTCGTCAAGTG+CGG + chr7_4:46790483-46790502 Msa1157710:CDS 50.0%
ACGTGATGGTCGTCAAGTAG+CGG + chr7_4:46789782-46789801 Msa1157710:CDS 50.0%
! GGTGTTGTGTGCTTGCATGA+TGG + chr7_4:46790470-46790489 Msa1157710:CDS 50.0%
TGCATGATGGTCGTCAAGTG+CGG + chr7_4:46790483-46790502 Msa1157710:CDS 50.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 46789717 46790508 46789717 ID=Msa1157710;Name=Msa1157710
chr7_4 mRNA 46789717 46790508 46789717 ID=Msa1157710-mRNA-1;Parent=Msa1157710;Name=Msa1157710-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|0|0|1|0|0|2|0|79
chr7_4 exon 46789717 46789903 46789717 ID=Msa1157710-mRNA-1:exon:12405;Parent=Msa1157710-mRNA-1
chr7_4 exon 46790456 46790508 46790456 ID=Msa1157710-mRNA-1:exon:12406;Parent=Msa1157710-mRNA-1
chr7_4 CDS 46789717 46789903 46789717 ID=Msa1157710-mRNA-1:cds;Parent=Msa1157710-mRNA-1
chr7_4 CDS 46790456 46790508 46790456 ID=Msa1157710-mRNA-1:cds;Parent=Msa1157710-mRNA-1
Gene Sequence

>Msa1157710

ATGACAAATACCTTTGAAGAGAAATTAGGTAAAGGAGGATTCAATGATGTGTACAATTCAAGCTTACGTGATGGTCGTCAAGTAGCGGTGAAAATAATAAATGATTCAAAGGGAGATGGAAAAAATTTCATAAATGAAGTAGATAAAATTAGTAAAACCTCACATGTGAACATCGCTTCACTTTTGGGTGAAGGAGGATTTGGTGTTGTGTGCTTGCATGATGGTCGTCAAGTGCGGTAA
Protein sequence

>Msa1157710

MTNTFEEKLGKGGFNDVYNSSLRDGRQVAVKIINDSKGDGKNFINEVDKISKTSHVNIASLLGEGGFGVVCLHDGRQVR*