AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1158460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1158460 MtrunA17_Chr7g0253141 99.620 263 1 0 1 263 1 263 0.0 553
Msa1158460 MtrunA17_Chr1g0169261 30.556 252 154 5 9 256 3 237 9.55e-32 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1158460 AT1G54390.4 78.707 263 51 4 1 261 1 260 5.20e-143 402
Msa1158460 AT1G54390.3 78.707 263 51 4 1 261 1 260 5.20e-143 402
Msa1158460 AT1G54390.7 75.111 225 51 4 39 261 19 240 7.17e-114 327
Msa1158460 AT1G54390.8 75.111 225 51 4 39 261 19 240 7.17e-114 327
Msa1158460 AT1G54390.5 75.111 225 51 4 39 261 19 240 7.17e-114 327
Msa1158460 AT1G54390.1 78.603 229 44 4 1 227 1 226 3.08e-113 328
Msa1158460 AT1G54390.2 77.729 229 46 4 1 227 1 226 3.90e-113 328
Msa1158460 AT1G54390.6 81.768 181 29 3 83 261 1 179 1.09e-99 289
Msa1158460 AT3G24010.1 31.452 248 144 6 9 256 3 224 7.87e-30 112

Find 91 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAATTTCAGGTATCTTT+TGG 0.176968 7_4:+47871248 Msa1158460:intron
ATTGCAATAGAAGAAATATT+AGG 0.220698 7_4:-47869278 None:intergenic
GTTGGGTTATGATTAATTAA+TGG 0.231604 7_4:+47871605 Msa1158460:three_prime_UTR
TGAACCTTGTCTCTTGTGTT+AGG 0.290251 7_4:-47871443 None:intergenic
TAGTCCTTCTTATGGCTTCC+TGG 0.301453 7_4:-47871056 None:intergenic
TTGCAATAGAAGAAATATTA+GGG 0.310592 7_4:-47869277 None:intergenic
TTGGGTTATGATTAATTAAT+GGG 0.310648 7_4:+47871606 Msa1158460:three_prime_UTR
AGTCTACGTCGATGACTATT+TGG 0.315508 7_4:+47869336 Msa1158460:CDS
CAATAATCATTCAATCAAAA+TGG 0.316662 7_4:+47869297 Msa1158460:exon
AATCGAAGAGACTTGATTAC+AGG 0.362393 7_4:+47870994 Msa1158460:CDS
ACACAGTATGTAGGTTCATT+TGG 0.368463 7_4:-47871113 None:intergenic
ACTTTGTCTGCTGCCTTGTC+TGG 0.368542 7_4:-47869697 None:intergenic
TGTAGGTTCATTTGGATCAA+TGG 0.378225 7_4:-47871105 None:intergenic
AAAAGAAATTGAGGCAAATC+AGG 0.389795 7_4:+47869820 Msa1158460:CDS
AACCCTGGAAGTGTTCTTGC+TGG 0.390369 7_4:-47869235 None:intergenic
GGATCATATAGTCCTTCTTA+TGG 0.391014 7_4:-47871064 None:intergenic
GAACCAGCAAGAACACTTCC+AGG 0.399537 7_4:+47869232 Msa1158460:five_prime_UTR
ACTTATGAACTTAGTTCAAC+AGG 0.416135 7_4:-47871528 None:intergenic
GTAGGTTCATTTGGATCAAT+GGG 0.417965 7_4:-47871104 None:intergenic
CTTTAAATCTTCAGCAAAGT+AGG 0.418687 7_4:-47870482 None:intergenic
AAATATTAGGGTTTGCGATT+GGG 0.421821 7_4:-47869265 None:intergenic
TACATACTGTGTTTGTCATC+AGG 0.423437 7_4:+47871124 Msa1158460:CDS
GAAATATTAGGGTTTGCGAT+TGG 0.424049 7_4:-47869266 None:intergenic
TTGATTACAGGGAGAGAGAC+TGG 0.434207 7_4:+47871006 Msa1158460:CDS
GAAAAGCGCAGGCACGTATA+TGG 0.434521 7_4:+47870963 Msa1158460:CDS
TTCTTATGGCTTCCTGGTGG+AGG 0.438248 7_4:-47871050 None:intergenic
CAAAACAACGTAAATATTCC+AGG 0.450586 7_4:+47869166 None:intergenic
TGTCACCGCATGGTGTGAAC+CGG 0.451384 7_4:-47871733 None:intergenic
AAGCTCAGCGGGCAATGTAT+TGG 0.453107 7_4:-47869459 None:intergenic
CGATTGGGATTTGCGAACCC+TGG 0.461059 7_4:-47869250 None:intergenic
GCAGCAGACAAAGTACTGTA+TGG 0.463935 7_4:+47869706 Msa1158460:CDS
AAAACGCTGTCGTTTGAGCC+TGG 0.464707 7_4:-47869184 None:intergenic
CTCATCTCATGGCTCTAAGA+AGG 0.470707 7_4:+47869733 Msa1158460:CDS
AGAAGTCTCTGAAGCTCAGC+GGG 0.472014 7_4:-47869470 None:intergenic
AACTAACCAGTTCATATGCT+TGG 0.476561 7_4:-47869886 None:intergenic
GTTTAGGGACAATAGGCAAT+GGG 0.494080 7_4:-47870938 None:intergenic
TTTGCTGAAGATTTAAAGCA+AGG 0.503317 7_4:+47870487 Msa1158460:CDS
GCGCGCCAACAAAACCTTCT+CGG 0.503510 7_4:-47869864 None:intergenic
GGCGCGCCAAGCATATGAAC+TGG 0.511810 7_4:+47869880 Msa1158460:CDS
TGTATGGGGTTCTCATCTCA+TGG 0.513570 7_4:+47869722 Msa1158460:CDS
AACACAAGAGACAAGGTTCA+AGG 0.514404 7_4:+47871446 Msa1158460:CDS
TTGCTTGACTATTTGTGTGC+CGG 0.515421 7_4:+47871714 Msa1158460:three_prime_UTR
GAAGGACTATATGATCCCTA+TGG 0.521889 7_4:+47871070 Msa1158460:CDS
CAGCAGACAAAGTACTGTAT+GGG 0.532458 7_4:+47869707 Msa1158460:CDS
CTGGGATTGGTCTGGTGTGA+GGG 0.532859 7_4:+47871837 Msa1158460:three_prime_UTR
ATGGGTTGATCAATATCCAT+AGG 0.533374 7_4:-47871086 None:intergenic
AAGGGAAAGAGTTACATAAG+AGG 0.534525 7_4:-47871648 None:intergenic
GATAGTGCCGAGGAGGTGAA+TGG 0.537405 7_4:+47871400 Msa1158460:intron
AAAATGGCAATTGCACGCAC+TGG 0.541228 7_4:+47869313 Msa1158460:exon
TAATCAAGTCTCTTCGATTG+AGG 0.541620 7_4:-47870990 None:intergenic
TTAGGCCAACACATGAATAG+TGG 0.541653 7_4:-47871425 None:intergenic
TTTAGGGACAATAGGCAATG+GGG 0.542772 7_4:-47870937 None:intergenic
TCCCTAAACCCGAAAAGCGC+AGG 0.544587 7_4:+47870952 Msa1158460:CDS
TAATAACAATTACATGAATG+AGG 0.550580 7_4:+47869769 Msa1158460:CDS
TCCACCAGGAAGCCATAAGA+AGG 0.551349 7_4:+47871052 Msa1158460:CDS
AAGATTTATCATTGACATTG+TGG 0.551966 7_4:-47871501 None:intergenic
CAGATACGAGGAAGCGAAGG+TGG 0.561608 7_4:-47869210 None:intergenic
TGGTTCCACTATTCATGTGT+TGG 0.563319 7_4:+47871420 Msa1158460:CDS
AGACAAGGTTCAAGGGAAAG+TGG 0.569480 7_4:+47871454 Msa1158460:CDS
ACACAAGAGACAAGGTTCAA+GGG 0.570451 7_4:+47871447 Msa1158460:CDS
TTAGGGACAATAGGCAATGG+GGG 0.570565 7_4:-47870936 None:intergenic
TCCTTCTTATGGCTTCCTGG+TGG 0.577394 7_4:-47871053 None:intergenic
GAAAATGCGAAAAGAAATTG+AGG 0.579696 7_4:+47869811 Msa1158460:CDS
ATTGAGTTTATGTACCGAGA+AGG 0.582404 7_4:+47869850 Msa1158460:CDS
TGTGGTAGTAATCTGCAAGT+TGG 0.587319 7_4:-47871483 None:intergenic
TTCTTGCTGGTTCAGATACG+AGG 0.590084 7_4:-47869222 None:intergenic
GGTTTAGGGACAATAGGCAA+TGG 0.590587 7_4:-47870939 None:intergenic
TGGGTTGATCAATATCCATA+GGG 0.596146 7_4:-47871085 None:intergenic
GAGAAGTCTCTGAAGCTCAG+CGG 0.598711 7_4:-47869471 None:intergenic
TCGTTCATCGAGTTCACGAA+CGG 0.599115 7_4:-47869498 None:intergenic
TCATCTCATGGCTCTAAGAA+GGG 0.599282 7_4:+47869734 Msa1158460:CDS
TGATGACAAACACAGTATGT+AGG 0.600981 7_4:-47871122 None:intergenic
TGGGTTATGATTAATTAATG+GGG 0.608426 7_4:+47871607 Msa1158460:three_prime_UTR
TTTGAGCTAATGCCTCCACC+AGG 0.610776 7_4:+47871038 Msa1158460:CDS
CCATGTAAAACGACTTGATG+AGG 0.613217 7_4:+47870453 Msa1158460:CDS
AGTGGAACCATTCACCTCCT+CGG 0.620021 7_4:-47871407 None:intergenic
TGATTACAGGGAGAGAGACT+GGG 0.622459 7_4:+47871007 Msa1158460:CDS
CCTTGACTAGAAAATACACG+TGG 0.624932 7_4:+47871667 Msa1158460:three_prime_UTR
ATGTCATCACTGTCACCGCA+TGG 0.625430 7_4:-47871743 None:intergenic
GTTCAGATACGAGGAAGCGA+AGG 0.630829 7_4:-47869213 None:intergenic
ATCGAAGAGACTTGATTACA+GGG 0.633832 7_4:+47870995 Msa1158460:CDS
ACTGGGATTGGTCTGGTGTG+AGG 0.634021 7_4:+47871836 Msa1158460:three_prime_UTR
AACCAGCAAGAACACTTCCA+GGG 0.642362 7_4:+47869233 Msa1158460:five_prime_UTR
AAGCTATGATAAACCAGACA+AGG 0.648028 7_4:+47869684 Msa1158460:intron
TATTATTCTTGATAGTGCCG+AGG 0.649316 7_4:+47871390 Msa1158460:intron
TATTCTTGATAGTGCCGAGG+AGG 0.654486 7_4:+47871393 Msa1158460:intron
AGCAGACAAAGTACTGTATG+GGG 0.659495 7_4:+47869708 Msa1158460:CDS
GGATTTGAGTAGTATGCACG+AGG 0.667663 7_4:+47871858 Msa1158460:three_prime_UTR
TTGGCCTAACACAAGAGACA+AGG 0.699970 7_4:+47871439 Msa1158460:CDS
GTGTGCCGGTTCACACCATG+CGG 0.732749 7_4:+47871728 Msa1158460:three_prime_UTR
CTTGACTAGAAAATACACGT+GGG 0.752169 7_4:+47871668 Msa1158460:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAAAAAAAGTAAATTTTTT+AGG - chr7_4:47869564-47869583 None:intergenic 10.0%
!!! AGTTTTTTTTTTAACTTTTA+TGG + chr7_4:47869905-47869924 Msa1158460:intron 10.0%
!!! TTTTATATATAGCATTTATA+AGG + chr7_4:47869941-47869960 Msa1158460:intron 10.0%
!! AAAAAAAAAGAAAATAAGAA+AGG - chr7_4:47870861-47870880 None:intergenic 10.0%
!! TGGAATAAAATTGAAAAATT+GGG - chr7_4:47869419-47869438 None:intergenic 15.0%
!! TTGGAATAAAATTGAAAAAT+TGG - chr7_4:47869420-47869439 None:intergenic 15.0%
!!! AATTTTTTAGGGTTAAAAAA+GGG - chr7_4:47869552-47869571 None:intergenic 15.0%
!!! AAATTTTTTAGGGTTAAAAA+AGG - chr7_4:47869553-47869572 None:intergenic 15.0%
!!! TTTTAATCATCATTTTTTGT+TGG + chr7_4:47869640-47869659 Msa1158460:intron 15.0%
!! AAAATATTAACAAAAAACAC+TGG + chr7_4:47871818-47871837 Msa1158460:three_prime_UTR 15.0%
!! AAATATTAACAAAAAACACT+GGG + chr7_4:47871819-47871838 Msa1158460:three_prime_UTR 15.0%
!! TTGCAATAGAAGAAATATTA+GGG - chr7_4:47869280-47869299 None:intergenic 20.0%
!! ATTGCAATAGAAGAAATATT+AGG - chr7_4:47869281-47869300 None:intergenic 20.0%
!! CAATAATCATTCAATCAAAA+TGG + chr7_4:47869297-47869316 Msa1158460:exon 20.0%
!! GAATAAAATTGAAAAATTGG+GGG - chr7_4:47869417-47869436 None:intergenic 20.0%
!! GGAATAAAATTGAAAAATTG+GGG - chr7_4:47869418-47869437 None:intergenic 20.0%
!!! AAATCTATTTTTAGATGCAA+TGG - chr7_4:47869620-47869639 None:intergenic 20.0%
!! TAATAACAATTACATGAATG+AGG + chr7_4:47869769-47869788 Msa1158460:CDS 20.0%
!!! TTTTTTTAACTTTTATGGCT+TGG + chr7_4:47869910-47869929 Msa1158460:intron 20.0%
!!! ATCAAAAATGAATGTTGTTT+TGG + chr7_4:47870745-47870764 Msa1158460:intron 20.0%
!!! TGTCAATGAAATTGTTTTTT+TGG + chr7_4:47870818-47870837 Msa1158460:intron 20.0%
!!! TTTTAGTGAATGCATAATTT+TGG + chr7_4:47871581-47871600 Msa1158460:three_prime_UTR 20.0%
!!! TTTAGTGAATGCATAATTTT+GGG + chr7_4:47871582-47871601 Msa1158460:three_prime_UTR 20.0%
!! TTGGGTTATGATTAATTAAT+GGG + chr7_4:47871606-47871625 Msa1158460:three_prime_UTR 20.0%
! CGTAGAGAATAGAAAATAAA+AGG - chr7_4:47869372-47869391 None:intergenic 25.0%
! AAAAATTGGGGGAAAAATTA+GGG - chr7_4:47869406-47869425 None:intergenic 25.0%
! CATCTATGGAATAGAAAAAT+TGG - chr7_4:47869439-47869458 None:intergenic 25.0%
! TTTGAGTAGGAATTTACATT+GGG - chr7_4:47869525-47869544 None:intergenic 25.0%
!!! TTTTGAGTAGGAATTTACAT+TGG - chr7_4:47869526-47869545 None:intergenic 25.0%
!!! AAAAAAGGGAATTTTTGAGT+AGG - chr7_4:47869538-47869557 None:intergenic 25.0%
! AGCAATTTCATATCAACAAA+GGG - chr7_4:47869587-47869606 None:intergenic 25.0%
! ACTTAATCACATTACCATTA+AGG - chr7_4:47870064-47870083 None:intergenic 25.0%
!!! AATTTTTTCCATATTGCTTC+TGG + chr7_4:47870356-47870375 Msa1158460:intron 25.0%
!!! GAAGAGTATGTTCAATATTT+TGG + chr7_4:47870646-47870665 Msa1158460:intron 25.0%
!!! TATTTTGGAAGTGCATAATT+TGG + chr7_4:47870661-47870680 Msa1158460:intron 25.0%
!!! TGAATGTTGTTTTGGAATTT+AGG + chr7_4:47870753-47870772 Msa1158460:intron 25.0%
!!! GAATGTTGTTTTGGAATTTA+GGG + chr7_4:47870754-47870773 Msa1158460:intron 25.0%
!!! AATGTTGTTTTGGAATTTAG+GGG + chr7_4:47870755-47870774 Msa1158460:intron 25.0%
! AACAATTTCATTGACAAAGA+AGG - chr7_4:47870814-47870833 None:intergenic 25.0%
! AACTCAGTCAAAATAACAAA+AGG - chr7_4:47871364-47871383 None:intergenic 25.0%
! AAGATTTATCATTGACATTG+TGG - chr7_4:47871504-47871523 None:intergenic 25.0%
!! TTAGTGAATGCATAATTTTG+GGG + chr7_4:47871583-47871602 Msa1158460:three_prime_UTR 25.0%
! GTTGGGTTATGATTAATTAA+TGG + chr7_4:47871605-47871624 Msa1158460:three_prime_UTR 25.0%
! TGGGTTATGATTAATTAATG+GGG + chr7_4:47871607-47871626 Msa1158460:three_prime_UTR 25.0%
AAATATTAGGGTTTGCGATT+GGG - chr7_4:47869268-47869287 None:intergenic 30.0%
GAAAAATTGGGGGAAAAATT+AGG - chr7_4:47869407-47869426 None:intergenic 30.0%
GCAATTTCATATCAACAAAG+GGG - chr7_4:47869586-47869605 None:intergenic 30.0%
GAGCAATTTCATATCAACAA+AGG - chr7_4:47869588-47869607 None:intergenic 30.0%
!!! ATCATCATTTTTTGTTGGTG+TGG + chr7_4:47869645-47869664 Msa1158460:intron 30.0%
! TTCTTTTCGCATTTTCTCAA+TGG - chr7_4:47869807-47869826 None:intergenic 30.0%
GAAAATGCGAAAAGAAATTG+AGG + chr7_4:47869811-47869830 Msa1158460:CDS 30.0%
AAAAGAAATTGAGGCAAATC+AGG + chr7_4:47869820-47869839 Msa1158460:CDS 30.0%
TGTGAATTATTGTTGCAACA+AGG + chr7_4:47869972-47869991 Msa1158460:intron 30.0%
GTGAATTATTGTTGCAACAA+GGG + chr7_4:47869973-47869992 Msa1158460:intron 30.0%
TGAATTATTGTTGCAACAAG+GGG + chr7_4:47869974-47869993 Msa1158460:intron 30.0%
ATAGTAATGAACGACCTTAA+TGG + chr7_4:47870047-47870066 Msa1158460:intron 30.0%
AGCTTAAACAAGAAGAATGA+TGG - chr7_4:47870237-47870256 None:intergenic 30.0%
ACAACTTATGTAACTGTCTT+CGG - chr7_4:47870283-47870302 None:intergenic 30.0%
AGTTACATAAGTTGTTTGAG+AGG + chr7_4:47870288-47870307 Msa1158460:intron 30.0%
CTTTAAATCTTCAGCAAAGT+AGG - chr7_4:47870485-47870504 None:intergenic 30.0%
! TTTGCTGAAGATTTAAAGCA+AGG + chr7_4:47870487-47870506 Msa1158460:CDS 30.0%
AAATCAGAATGCAAGTCAAA+TGG - chr7_4:47870521-47870540 None:intergenic 30.0%
CCAGATCATACTAATTGTAT+AGG - chr7_4:47870581-47870600 None:intergenic 30.0%
CCTATACAATTAGTATGATC+TGG + chr7_4:47870578-47870597 Msa1158460:intron 30.0%
CATTACATGTTTCTTTAGAC+AGG - chr7_4:47870697-47870716 None:intergenic 30.0%
TCATTGACAAAGAAGGAAAA+GGG - chr7_4:47870807-47870826 None:intergenic 30.0%
TTCATTGACAAAGAAGGAAA+AGG - chr7_4:47870808-47870827 None:intergenic 30.0%
!! TTCACATTTGATTTTGTCAG+AGG + chr7_4:47870881-47870900 Msa1158460:intron 30.0%
!! TCACATTTGATTTTGTCAGA+GGG + chr7_4:47870882-47870901 Msa1158460:intron 30.0%
AGTCAGTTTACTGAAATTTC+AGG + chr7_4:47871238-47871257 Msa1158460:intron 30.0%
!! CTGAAATTTCAGGTATCTTT+TGG + chr7_4:47871248-47871267 Msa1158460:intron 30.0%
GTCAAAATAACAAAAGGAGA+GGG - chr7_4:47871358-47871377 None:intergenic 30.0%
AGTCAAAATAACAAAAGGAG+AGG - chr7_4:47871359-47871378 None:intergenic 30.0%
ACTTATGAACTTAGTTCAAC+AGG - chr7_4:47871531-47871550 None:intergenic 30.0%
TTAACAAAAAACACTGGGAT+TGG + chr7_4:47871824-47871843 Msa1158460:three_prime_UTR 30.0%
GAAATATTAGGGTTTGCGAT+TGG - chr7_4:47869269-47869288 None:intergenic 35.0%
AAGCTATGATAAACCAGACA+AGG + chr7_4:47869684-47869703 Msa1158460:intron 35.0%
ATTGAGTTTATGTACCGAGA+AGG + chr7_4:47869850-47869869 Msa1158460:CDS 35.0%
AACTAACCAGTTCATATGCT+TGG - chr7_4:47869889-47869908 None:intergenic 35.0%
!! AACTTTTATGGCTTGGACTA+AGG + chr7_4:47869917-47869936 Msa1158460:intron 35.0%
!! ACTTTTATGGCTTGGACTAA+GGG + chr7_4:47869918-47869937 Msa1158460:intron 35.0%
GTTCAGAAATGTAAATCCCT+AGG - chr7_4:47870000-47870019 None:intergenic 35.0%
! TCACTGTACAATTTTTCTCG+AGG + chr7_4:47870207-47870226 Msa1158460:intron 35.0%
ATGCATAACCAGAAGCAATA+TGG - chr7_4:47870367-47870386 None:intergenic 35.0%
CATTGACAAAGAAGGAAAAG+GGG - chr7_4:47870806-47870825 None:intergenic 35.0%
!! CACATTTGATTTTGTCAGAG+GGG + chr7_4:47870883-47870902 Msa1158460:intron 35.0%
TAATCAAGTCTCTTCGATTG+AGG - chr7_4:47870993-47871012 None:intergenic 35.0%
AATCGAAGAGACTTGATTAC+AGG + chr7_4:47870994-47871013 Msa1158460:CDS 35.0%
ATCGAAGAGACTTGATTACA+GGG + chr7_4:47870995-47871014 Msa1158460:CDS 35.0%
GGATCATATAGTCCTTCTTA+TGG - chr7_4:47871067-47871086 None:intergenic 35.0%
!! TGGGTTGATCAATATCCATA+GGG - chr7_4:47871088-47871107 None:intergenic 35.0%
!! ATGGGTTGATCAATATCCAT+AGG - chr7_4:47871089-47871108 None:intergenic 35.0%
GTAGGTTCATTTGGATCAAT+GGG - chr7_4:47871107-47871126 None:intergenic 35.0%
TGTAGGTTCATTTGGATCAA+TGG - chr7_4:47871108-47871127 None:intergenic 35.0%
ACACAGTATGTAGGTTCATT+TGG - chr7_4:47871116-47871135 None:intergenic 35.0%
TGATGACAAACACAGTATGT+AGG - chr7_4:47871125-47871144 None:intergenic 35.0%
TACATACTGTGTTTGTCATC+AGG + chr7_4:47871124-47871143 Msa1158460:CDS 35.0%
! TATAGTTTCAAGTCTCCTTG+TGG + chr7_4:47871176-47871195 Msa1158460:intron 35.0%
ATATGACACAATTGTCCACA+AGG - chr7_4:47871194-47871213 None:intergenic 35.0%
!! TATTATTCTTGATAGTGCCG+AGG + chr7_4:47871390-47871409 Msa1158460:intron 35.0%
!!! TGAATGCATAATTTTGGGGT+TGG + chr7_4:47871587-47871606 Msa1158460:three_prime_UTR 35.0%
!!! GAATGCATAATTTTGGGGTT+GGG + chr7_4:47871588-47871607 Msa1158460:three_prime_UTR 35.0%
AAGGGAAAGAGTTACATAAG+AGG - chr7_4:47871651-47871670 None:intergenic 35.0%
!! CACGTGTATTTTCTAGTCAA+GGG - chr7_4:47871669-47871688 None:intergenic 35.0%
CTTGACTAGAAAATACACGT+GGG + chr7_4:47871668-47871687 Msa1158460:three_prime_UTR 35.0%
AGTCTACGTCGATGACTATT+TGG + chr7_4:47869336-47869355 Msa1158460:CDS 40.0%
TGGGGGAAAAATTAGGGTTA+GGG - chr7_4:47869400-47869419 None:intergenic 40.0%
TTGGGGGAAAAATTAGGGTT+AGG - chr7_4:47869401-47869420 None:intergenic 40.0%
!! CAGCAGACAAAGTACTGTAT+GGG + chr7_4:47869707-47869726 Msa1158460:CDS 40.0%
!! AGCAGACAAAGTACTGTATG+GGG + chr7_4:47869708-47869727 Msa1158460:CDS 40.0%
!! TCATCTCATGGCTCTAAGAA+GGG + chr7_4:47869734-47869753 Msa1158460:CDS 40.0%
!! ATGTACCGAGAAGGTTTTGT+TGG + chr7_4:47869859-47869878 Msa1158460:CDS 40.0%
ATGATTGCAGCTCCTATCTT+GGG + chr7_4:47870324-47870343 Msa1158460:intron 40.0%
ATTCACAAGACTCCCAAGAT+AGG - chr7_4:47870339-47870358 None:intergenic 40.0%
!! CCTCATCAAGTCGTTTTACA+TGG - chr7_4:47870456-47870475 None:intergenic 40.0%
CCATGTAAAACGACTTGATG+AGG + chr7_4:47870453-47870472 Msa1158460:CDS 40.0%
AAGGAAAAGGGGAATTTCAG+GGG - chr7_4:47870795-47870814 None:intergenic 40.0%
GAAGGAAAAGGGGAATTTCA+GGG - chr7_4:47870796-47870815 None:intergenic 40.0%
AGAAGGAAAAGGGGAATTTC+AGG - chr7_4:47870797-47870816 None:intergenic 40.0%
TTTAGGGACAATAGGCAATG+GGG - chr7_4:47870940-47870959 None:intergenic 40.0%
GTTTAGGGACAATAGGCAAT+GGG - chr7_4:47870941-47870960 None:intergenic 40.0%
!!! TTTTCGGGTTTAGGGACAAT+AGG - chr7_4:47870948-47870967 None:intergenic 40.0%
GAAGGACTATATGATCCCTA+TGG + chr7_4:47871070-47871089 Msa1158460:CDS 40.0%
TGGTTCCACTATTCATGTGT+TGG + chr7_4:47871420-47871439 Msa1158460:CDS 40.0%
TTAGGCCAACACATGAATAG+TGG - chr7_4:47871428-47871447 None:intergenic 40.0%
TGAACCTTGTCTCTTGTGTT+AGG - chr7_4:47871446-47871465 None:intergenic 40.0%
AACACAAGAGACAAGGTTCA+AGG + chr7_4:47871446-47871465 Msa1158460:CDS 40.0%
ACACAAGAGACAAGGTTCAA+GGG + chr7_4:47871447-47871466 Msa1158460:CDS 40.0%
TGTGGTAGTAATCTGCAAGT+TGG - chr7_4:47871486-47871505 None:intergenic 40.0%
!! CCACGTGTATTTTCTAGTCA+AGG - chr7_4:47871670-47871689 None:intergenic 40.0%
CCTTGACTAGAAAATACACG+TGG + chr7_4:47871667-47871686 Msa1158460:three_prime_UTR 40.0%
TTGCTTGACTATTTGTGTGC+CGG + chr7_4:47871714-47871733 Msa1158460:three_prime_UTR 40.0%
!! AAAAAACACTGGGATTGGTC+TGG + chr7_4:47871829-47871848 Msa1158460:three_prime_UTR 40.0%
!! TTCTTGCTGGTTCAGATACG+AGG - chr7_4:47869225-47869244 None:intergenic 45.0%
AACCAGCAAGAACACTTCCA+GGG + chr7_4:47869233-47869252 Msa1158460:five_prime_UTR 45.0%
AAAATGGCAATTGCACGCAC+TGG + chr7_4:47869313-47869332 Msa1158460:exon 45.0%
! GGGCAATGTATTGGCATCTA+TGG - chr7_4:47869453-47869472 None:intergenic 45.0%
TCGTTCATCGAGTTCACGAA+CGG - chr7_4:47869501-47869520 None:intergenic 45.0%
!! GCAGCAGACAAAGTACTGTA+TGG + chr7_4:47869706-47869725 Msa1158460:CDS 45.0%
TGTATGGGGTTCTCATCTCA+TGG + chr7_4:47869722-47869741 Msa1158460:CDS 45.0%
!! CTCATCTCATGGCTCTAAGA+AGG + chr7_4:47869733-47869752 Msa1158460:CDS 45.0%
GATGATTGCAGCTCCTATCT+TGG + chr7_4:47870323-47870342 Msa1158460:intron 45.0%
TTAGGGACAATAGGCAATGG+GGG - chr7_4:47870939-47870958 None:intergenic 45.0%
GGTTTAGGGACAATAGGCAA+TGG - chr7_4:47870942-47870961 None:intergenic 45.0%
! ATATACGTGCCTGCGCTTTT+CGG - chr7_4:47870964-47870983 None:intergenic 45.0%
TTGATTACAGGGAGAGAGAC+TGG + chr7_4:47871006-47871025 Msa1158460:CDS 45.0%
TGATTACAGGGAGAGAGACT+GGG + chr7_4:47871007-47871026 Msa1158460:CDS 45.0%
TAGTCCTTCTTATGGCTTCC+TGG - chr7_4:47871059-47871078 None:intergenic 45.0%
TGAACTTTGCTCTCTTGTGC+TGG + chr7_4:47871309-47871328 Msa1158460:intron 45.0%
!! TATTCTTGATAGTGCCGAGG+AGG + chr7_4:47871393-47871412 Msa1158460:intron 45.0%
TTGGCCTAACACAAGAGACA+AGG + chr7_4:47871439-47871458 Msa1158460:CDS 45.0%
AGACAAGGTTCAAGGGAAAG+TGG + chr7_4:47871454-47871473 Msa1158460:CDS 45.0%
GGATTTGAGTAGTATGCACG+AGG + chr7_4:47871858-47871877 Msa1158460:three_prime_UTR 45.0%
!!! TAAAAAAAGTAAATTTTTTA+GGG - chr7_4:47869563-47869582 None:intergenic 5.0%
! AAAACGCTGTCGTTTGAGCC+TGG - chr7_4:47869187-47869206 None:intergenic 50.0%
GTTCAGATACGAGGAAGCGA+AGG - chr7_4:47869216-47869235 None:intergenic 50.0%
GAACCAGCAAGAACACTTCC+AGG + chr7_4:47869232-47869251 Msa1158460:five_prime_UTR 50.0%
!! AACCCTGGAAGTGTTCTTGC+TGG - chr7_4:47869238-47869257 None:intergenic 50.0%
AAGCTCAGCGGGCAATGTAT+TGG - chr7_4:47869462-47869481 None:intergenic 50.0%
!! AGAAGTCTCTGAAGCTCAGC+GGG - chr7_4:47869473-47869492 None:intergenic 50.0%
!! GAGAAGTCTCTGAAGCTCAG+CGG - chr7_4:47869474-47869493 None:intergenic 50.0%
ACTTTGTCTGCTGCCTTGTC+TGG - chr7_4:47869700-47869719 None:intergenic 50.0%
ATTGTTGCAACAAGGGGCCT+AGG + chr7_4:47869980-47869999 Msa1158460:intron 50.0%
TTGTTGCAACAAGGGGCCTA+GGG + chr7_4:47869981-47870000 Msa1158460:intron 50.0%
! TATACGTGCCTGCGCTTTTC+GGG - chr7_4:47870963-47870982 None:intergenic 50.0%
! GAAAAGCGCAGGCACGTATA+TGG + chr7_4:47870963-47870982 Msa1158460:CDS 50.0%
TTTGAGCTAATGCCTCCACC+AGG + chr7_4:47871038-47871057 Msa1158460:CDS 50.0%
TTCTTATGGCTTCCTGGTGG+AGG - chr7_4:47871053-47871072 None:intergenic 50.0%
TCCACCAGGAAGCCATAAGA+AGG + chr7_4:47871052-47871071 Msa1158460:CDS 50.0%
TCCTTCTTATGGCTTCCTGG+TGG - chr7_4:47871056-47871075 None:intergenic 50.0%
AGTGGAACCATTCACCTCCT+CGG - chr7_4:47871410-47871429 None:intergenic 50.0%
ATGTCATCACTGTCACCGCA+TGG - chr7_4:47871746-47871765 None:intergenic 50.0%
CAGATACGAGGAAGCGAAGG+TGG - chr7_4:47869213-47869232 None:intergenic 55.0%
CGATTGGGATTTGCGAACCC+TGG - chr7_4:47869253-47869272 None:intergenic 55.0%
GCGCGCCAACAAAACCTTCT+CGG - chr7_4:47869867-47869886 None:intergenic 55.0%
GGCAATGGGGGAAGAATTGC+TGG - chr7_4:47870927-47870946 None:intergenic 55.0%
! TCCCTAAACCCGAAAAGCGC+AGG + chr7_4:47870952-47870971 Msa1158460:CDS 55.0%
!!! GCCTGCGCTTTTCGGGTTTA+GGG - chr7_4:47870956-47870975 None:intergenic 55.0%
!!! TGCCTGCGCTTTTCGGGTTT+AGG - chr7_4:47870957-47870976 None:intergenic 55.0%
! GATAGTGCCGAGGAGGTGAA+TGG + chr7_4:47871400-47871419 Msa1158460:intron 55.0%
TGTCACCGCATGGTGTGAAC+CGG - chr7_4:47871736-47871755 None:intergenic 55.0%
!! ACTGGGATTGGTCTGGTGTG+AGG + chr7_4:47871836-47871855 Msa1158460:three_prime_UTR 55.0%
!! CTGGGATTGGTCTGGTGTGA+GGG + chr7_4:47871837-47871856 Msa1158460:three_prime_UTR 55.0%
GGCGCGCCAAGCATATGAAC+TGG + chr7_4:47869880-47869899 Msa1158460:CDS 60.0%
!! GTGTGCCGGTTCACACCATG+CGG + chr7_4:47871728-47871747 Msa1158460:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 47869168 47871891 47869168 ID=Msa1158460;Name=Msa1158460
chr7_4 mRNA 47869168 47871891 47869168 ID=Msa1158460-mRNA-1;Parent=Msa1158460;Name=Msa1158460-mRNA-1;_AED=0.21;_eAED=0.21;_QI=148|1|1|1|1|1|7|376|263
chr7_4 exon 47869168 47869361 47869168 ID=Msa1158460-mRNA-1:exon:12783;Parent=Msa1158460-mRNA-1
chr7_4 exon 47869456 47869527 47869456 ID=Msa1158460-mRNA-1:exon:12784;Parent=Msa1158460-mRNA-1
chr7_4 exon 47869687 47869901 47869687 ID=Msa1158460-mRNA-1:exon:12785;Parent=Msa1158460-mRNA-1
chr7_4 exon 47870445 47870508 47870445 ID=Msa1158460-mRNA-1:exon:12786;Parent=Msa1158460-mRNA-1
chr7_4 exon 47870901 47871145 47870901 ID=Msa1158460-mRNA-1:exon:12787;Parent=Msa1158460-mRNA-1
chr7_4 exon 47871260 47871298 47871260 ID=Msa1158460-mRNA-1:exon:12788;Parent=Msa1158460-mRNA-1
chr7_4 exon 47871405 47871891 47871405 ID=Msa1158460-mRNA-1:exon:12789;Parent=Msa1158460-mRNA-1
chr7_4 five_prime_UTR 47869168 47869315 47869168 ID=Msa1158460-mRNA-1:five_prime_utr;Parent=Msa1158460-mRNA-1
chr7_4 CDS 47869316 47869361 47869316 ID=Msa1158460-mRNA-1:cds;Parent=Msa1158460-mRNA-1
chr7_4 CDS 47869456 47869527 47869456 ID=Msa1158460-mRNA-1:cds;Parent=Msa1158460-mRNA-1
chr7_4 CDS 47869687 47869901 47869687 ID=Msa1158460-mRNA-1:cds;Parent=Msa1158460-mRNA-1
chr7_4 CDS 47870445 47870508 47870445 ID=Msa1158460-mRNA-1:cds;Parent=Msa1158460-mRNA-1
chr7_4 CDS 47870901 47871145 47870901 ID=Msa1158460-mRNA-1:cds;Parent=Msa1158460-mRNA-1
chr7_4 CDS 47871260 47871298 47871260 ID=Msa1158460-mRNA-1:cds;Parent=Msa1158460-mRNA-1
chr7_4 CDS 47871405 47871515 47871405 ID=Msa1158460-mRNA-1:cds;Parent=Msa1158460-mRNA-1
chr7_4 three_prime_UTR 47871516 47871891 47871516 ID=Msa1158460-mRNA-1:three_prime_utr;Parent=Msa1158460-mRNA-1
Gene Sequence

>Msa1158460

ATGGCAATTGCACGCACTGGAGTCTACGTCGATGACTATTTGGAGTATGCCAATACATTGCCCGCTGAGCTTCAGAGACTTCTCAACACCGTTCGTGAACTCGATGAACGATCCCAATCTATGATAAACCAGACAAGGCAGCAGACAAAGTACTGTATGGGGTTCTCATCTCATGGCTCTAAGAAGGGTAATCATAATTATAATAACAATTACATGAATGAGGATGATGATGCTTCCATTGAGAAAATGCGAAAAGAAATTGAGGCAAATCAGGACAGTGCATTGAGTTTATGTACCGAGAAGGTTTTGTTGGCGCGCCAAGCATATGAACTGATAGACAGCCATGTAAAACGACTTGATGAGGATTTAACCTACTTTGCTGAAGATTTAAAGCAAGAGGGGAAAATATCACAAGATGAGCCAGCAATTCTTCCCCCATTGCCTATTGTCCCTAAACCCGAAAAGCGCAGGCACGTATATGGTACACCTCAATCGAAGAGACTTGATTACAGGGAGAGAGACTGGGATAGAGACTTTGAGCTAATGCCTCCACCAGGAAGCCATAAGAAGGACTATATGATCCCTATGGATATTGATCAACCCATTGATCCAAATGAACCTACATACTGTGTTTGTCATCAGGTATCTTTTGGAGATATGATTGCTTGTGACAACGAAAATTGCCGAGGAGGTGAATGGTTCCACTATTCATGTGTTGGCCTAACACAAGAGACAAGGTTCAAGGGAAAGTGGTATTGTCCAACTTGCAGATTACTACCACAATGTCAATGA
Protein sequence

>Msa1158460

MAIARTGVYVDDYLEYANTLPAELQRLLNTVRELDERSQSMINQTRQQTKYCMGFSSHGSKKGNHNYNNNYMNEDDDASIEKMRKEIEANQDSALSLCTEKVLLARQAYELIDSHVKRLDEDLTYFAEDLKQEGKISQDEPAILPPLPIVPKPEKRRHVYGTPQSKRLDYRERDWDRDFELMPPPGSHKKDYMIPMDIDQPIDPNEPTYCVCHQVSFGDMIACDNENCRGGEWFHYSCVGLTQETRFKGKWYCPTCRLLPQCQ*