AlfalfaGEDB | Msa1162350

M. sativa cultivar zhongmu-4 / Msa1162350

KEGG: -
GO: GO:0000003 GO:0000976 GO:0000981 GO:0001046 GO:0001047 GO:0001067 GO:0001228 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1162350	XP_003625199.1	89.752	322	23	3	1	315	1	319	0.0	586

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1162350	sp\|Q66GR3\|BH130_ARATH	48.133	241	106	6	84	315	125	355	5.53e-49	170

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1162350	G7KUF7	89.752	322	23	3	1	315	1	319	0.0	586

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TFs/TRs:

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Gene ID	Type	Classification
Msa1162350	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1162350	MtrunA17_Chr7g0258181	89.752	322	23	3	1	315	1	319	0.0	586
Msa1162350	MtrunA17_Chr1g0174271	46.505	372	128	9	4	311	6	370	8.07e-85	260
Msa1162350	MtrunA17_Chr4g0032951	39.015	264	126	6	81	315	174	431	2.17e-50	172
Msa1162350	MtrunA17_Chr2g0329041	43.137	255	119	8	83	315	157	407	8.99e-48	165
Msa1162350	MtrunA17_Chr3g0087771	38.618	246	112	6	88	311	166	394	4.69e-42	150
Msa1162350	MtrunA17_Chr8g0351881	39.200	250	111	5	88	311	154	388	2.51e-35	131
Msa1162350	MtrunA17_Chr4g0014771	69.620	79	24	0	233	311	112	190	1.64e-33	122
Msa1162350	MtrunA17_Chr1g0152131	52.113	71	34	0	239	309	260	330	4.61e-16	78.2
Msa1162350	MtrunA17_Chr4g0074161	54.412	68	30	1	239	306	206	272	5.53e-16	78.2
Msa1162350	MtrunA17_Chr1g0190731	50.667	75	36	1	232	306	186	259	1.09e-15	76.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1162350	AT2G42280.1	48.133	241	106	6	84	315	125	355	5.62e-50	170
Msa1162350	AT2G42280.3	48.458	227	98	6	84	301	125	341	6.32e-44	154
Msa1162350	AT1G51140.1	40.083	242	110	8	89	311	151	376	1.46e-40	145
Msa1162350	AT2G43140.1	57.143	105	44	1	208	311	102	206	7.01e-35	126
Msa1162350	AT2G43140.2	57.143	105	44	1	208	311	199	303	2.53e-34	127
Msa1162350	AT1G35460.1	61.176	85	33	0	231	315	173	257	3.08e-32	120
Msa1162350	AT1G05805.1	56.731	104	43	2	209	311	253	355	9.47e-31	119
Msa1162350	AT4G09180.1	59.341	91	35	1	225	315	172	260	3.87e-30	115
Msa1162350	AT1G35460.2	76.596	47	11	0	231	277	173	219	4.56e-18	82.0
Msa1162350	AT2G24260.2	50.000	78	38	1	229	306	25	101	2.86e-16	77.4

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Find 56 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGAAGCAACCTTGAATT+TGG	0.214893	7_4:-53030209	None:intergenic
AAAGGAGATGATGTTGTTGT+TGG	0.459546	7_4:-53030471	None:intergenic
AAAGGTGTTGAAAACTATGA+TGG	0.487777	7_4:+53031291	Msa1162350:CDS
AACAAAACCAACCAAATTCA+AGG	0.457834	7_4:+53030198	Msa1162350:CDS
AACAACAACATCATCTCCTT+TGG	0.393746	7_4:+53030473	Msa1162350:CDS
AACATATTGAACAATCAAAT+TGG	0.290694	7_4:+53031351	Msa1162350:CDS
AACATCATCTCCTTTGGAAA+TGG	0.414195	7_4:+53030479	Msa1162350:CDS
AACATGGGGAGTGATGGCGT+TGG	0.638657	7_4:+53031423	Msa1162350:CDS
AACTCTAACATGGGGAGTGA+TGG	0.529452	7_4:+53031417	Msa1162350:CDS
AAGACACATGAATTCTCAGC+AGG	0.502231	7_4:+53030373	Msa1162350:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAAAACCAACCAAATTCA+AGG	+	chr7_4:53030198-53030217	Msa1162350:CDS	30.0%
TACTACAATACCATTTCCAA+AGG	-	chr7_4:53030492-53030511	None:intergenic	30.0%
AAGTTATTTATGTACAAGGG+AGG	+	chr7_4:53031032-53031051	Msa1162350:intron	30.0%
AGTTATTTATGTACAAGGGA+GGG	+	chr7_4:53031033-53031052	Msa1162350:intron	30.0%
CTAATGTGATTCATATTGCT+AGG	-	chr7_4:53031209-53031228	None:intergenic	30.0%
TTAACACAGAACTCTAACAT+GGG	+	chr7_4:53031408-53031427	Msa1162350:CDS	30.0%
TTCTGTTATACTCAGAATGT+AGG	+	chr7_4:53031613-53031632	Msa1162350:intron	30.0%
TCTGTTATACTCAGAATGTA+GGG	+	chr7_4:53031614-53031633	Msa1162350:intron	30.0%
GATTGTTCCAAACATTGATA+AGG	+	chr7_4:53031843-53031862	Msa1162350:CDS	30.0%
ATTCTGACCTTATCAATGTT+TGG	-	chr7_4:53031853-53031872	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	53030167	53032189	53030167	ID=Msa1162350;Name=Msa1162350
chr7_4	mRNA	53030167	53032189	53030167	ID=Msa1162350-mRNA-1;Parent=Msa1162350;Name=Msa1162350-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0\|0\|0\|1\|1\|1\|5\|0\|315
chr7_4	exon	53030167	53030500	53030167	ID=Msa1162350-mRNA-1:exon:15275;Parent=Msa1162350-mRNA-1
chr7_4	exon	53031277	53031536	53031277	ID=Msa1162350-mRNA-1:exon:15276;Parent=Msa1162350-mRNA-1
chr7_4	exon	53031628	53031864	53031628	ID=Msa1162350-mRNA-1:exon:15277;Parent=Msa1162350-mRNA-1
chr7_4	exon	53031961	53032029	53031961	ID=Msa1162350-mRNA-1:exon:15278;Parent=Msa1162350-mRNA-1
chr7_4	exon	53032142	53032189	53032142	ID=Msa1162350-mRNA-1:exon:15279;Parent=Msa1162350-mRNA-1
chr7_4	CDS	53030167	53030500	53030167	ID=Msa1162350-mRNA-1:cds;Parent=Msa1162350-mRNA-1
chr7_4	CDS	53031277	53031536	53031277	ID=Msa1162350-mRNA-1:cds;Parent=Msa1162350-mRNA-1
chr7_4	CDS	53031628	53031864	53031628	ID=Msa1162350-mRNA-1:cds;Parent=Msa1162350-mRNA-1
chr7_4	CDS	53031961	53032029	53031961	ID=Msa1162350-mRNA-1:cds;Parent=Msa1162350-mRNA-1
chr7_4	CDS	53032142	53032189	53032142	ID=Msa1162350-mRNA-1:cds;Parent=Msa1162350-mRNA-1

Gene Sequence

>Msa1162350

ATGGATTTGAATTCTCATTATCAACAACTCCAACAAAACCAACCAAATTCAAGGTTGCTTCGTTTTCGTTCAAGTTTCAAACAAGCTGAAGGTTGTGCTACAAATTCTGGTGAAACTTCTTCATCTACCTTTAGAGAATTCATGGACCATAATCCTTTTAATCATAAAGGTGACAACAACAACAACAACGAGTCTTCATTATCTCAAAGACACATGAATTCTCAGCAGGGTTGTAGAATGGATCAACACAAAGGTTTTTATACCAATCTTTTAAGACAGAGTGGTTCCCATGCTGGTCATTTCTCCAACAACAACATCATCTCCTTTGGAAATGGGTATGAATCTATGAAAGGTGTTGAAAACTATGATGGGGTGAATGACAGTGATGGTGAACTTACTCTATCTATGAACATATTGAACAATCAAATTGGCTTCTCACCAAGAAGTCCTTCTTCCTTGAGAATGTTAACACAGAACTCTAACATGGGGAGTGATGGCGTTGGAGCAACTAGCCGTGATGATCGAAGACAAGTAGGTAGCAATGACGATGCTCAACATTATGGTCATAAATTCATTTATGATTCTAATGATAAGAATGTAGGGGTACGAAATCAGGTTGATACACTATCACATCACTTGAGTTTGCCAAGAAAATCATCAGAAATGTTTGTTGTGGAGAATTTGATTCAGTTTCCAGATTCTGTTCCAAGCAGTATCAGAGCAAAGAGAGGCTTTGCAACACATCCTAGAAGCCTTGCCGAAAGGGTAAGAAGAACTCGGATAAGTGAACGAATGAGAAAATTGCAAGAGATTGTTCCAAACATTGATAAGCAAACTTGCACATCAGAAATGTTAGACTTGGCTGTAGAGTACATTAAAGATCTTCAAAAACAATTAAAGACTATGAGTGCAAAAAGAGCTAAGTGCAGATGCAGAAACAAGAAATGA

Protein sequence

>Msa1162350

MDLNSHYQQLQQNQPNSRLLRFRSSFKQAEGCATNSGETSSSTFREFMDHNPFNHKGDNNNNNESSLSQRHMNSQQGCRMDQHKGFYTNLLRQSGSHAGHFSNNNIISFGNGYESMKGVENYDGVNDSDGELTLSMNILNNQIGFSPRSPSSLRMLTQNSNMGSDGVGATSRDDRRQVGSNDDAQHYGHKFIYDSNDKNVGVRNQVDTLSHHLSLPRKSSEMFVVENLIQFPDSVPSSIRAKRGFATHPRSLAERVRRTRISERMRKLQEIVPNIDKQTCTSEMLDLAVEYIKDLQKQLKTMSAKRAKCRCRNKK*