AlfalfaGEDB | Msa1163080

M. sativa cultivar zhongmu-4 / Msa1163080

KEGG: -
GO: GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0036211 GO:0038023 GO:0040007 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0044706 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051716 GO:0060089 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:0140096 GO:1901564 GO:1905392
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1163080	AES81514.2	89.011	364	8	1	1	332	1	364	0.0	660

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1163080	sp\|P93749\|Y2197_ARATH	75.477	367	53	5	1	332	1	365	0.0	532

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1163080	G7KWA4	89.011	364	8	1	1	332	1	364	0.0	660

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1163080	MtrunA17_Chr7g0258951	88.043	368	8	1	1	332	1	368	0.0	659
Msa1163080	MtrunA17_Chr1g0175731	74.462	372	55	3	1	332	1	372	0.0	515
Msa1163080	MtrunA17_Chr1g0213501	76.531	294	68	1	37	330	111	403	5.13e-167	471
Msa1163080	MtrunA17_Chr1g0194101	72.727	297	76	2	37	331	68	361	1.00e-155	440
Msa1163080	MtrunA17_Chr4g0069241	70.979	286	80	1	37	322	67	349	8.97e-147	417
Msa1163080	MtrunA17_Chr3g0084191	59.386	293	97	3	37	329	227	497	2.67e-118	350
Msa1163080	MtrunA17_Chr0c20g0493811	59.109	247	60	2	85	322	29	243	2.79e-96	286
Msa1163080	MtrunA17_Chr2g0289161	45.455	286	150	2	37	321	419	699	5.02e-82	263
Msa1163080	MtrunA17_Chr7g0262521	47.552	286	142	4	38	322	346	624	3.73e-81	261
Msa1163080	MtrunA17_Chr7g0270121	47.719	285	139	5	37	318	379	656	7.74e-81	260

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1163080	AT2G41970.1	75.477	367	53	5	1	332	1	365	0.0	532
Msa1163080	AT3G59350.2	75.945	291	69	1	37	327	70	359	7.96e-162	456
Msa1163080	AT3G59350.6	75.945	291	69	1	37	327	127	416	2.12e-161	457
Msa1163080	AT3G59350.3	75.945	291	69	1	37	327	112	401	5.47e-161	456
Msa1163080	AT3G59350.1	75.945	291	69	1	37	327	112	401	5.47e-161	456
Msa1163080	AT1G06700.1	75.524	286	69	1	37	322	67	351	1.19e-160	453
Msa1163080	AT1G06700.3	75.524	286	69	1	37	322	67	351	1.19e-160	453
Msa1163080	AT1G06700.2	75.524	286	69	1	37	322	67	351	1.19e-160	453
Msa1163080	AT2G43230.1	75.601	291	70	1	37	327	110	399	2.28e-160	454
Msa1163080	AT2G30740.5	75.175	286	68	2	37	321	70	353	2.42e-160	452

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Find 78 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGATGTTTATAGTTT+TGG	0.205463	7_4:-54148409	Msa1163080:CDS
AAACCTTAGCAATTGCCTTT+GGG	0.341988	7_4:+54148122	None:intergenic
AACAGGAAACTTTGGTTCAA+AGG	0.456129	7_4:-54149594	Msa1163080:intron
AACCTTAGCAATTGCCTTTG+GGG	0.525433	7_4:+54148123	None:intergenic
AAGTCGTTGGAGTCAGAATC+AGG	0.418607	7_4:+54149476	None:intergenic
AATACGCTGCACCATCAAGA+GGG	0.602640	7_4:-54150013	Msa1163080:CDS
AATAGATTAACAGGAAACTT+TGG	0.423697	7_4:-54149602	Msa1163080:intron
AATGCATATGGTGGCGGCGG+CGG	0.413682	7_4:-54149987	Msa1163080:CDS
ACAAGACTTTGTTGCCCTTT+AGG	0.278654	7_4:+54148334	None:intergenic
ACAAGAGTCTTGGGAACATT+TGG	0.353209	7_4:-54148737	Msa1163080:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCATTGCCTATATTCATACA+AGG	+	chr7_4:54147497-54147516	None:intergenic	30.0%
ATGAATATAGGCAATGATAG+AGG	-	chr7_4:54147500-54147519	Msa1163080:intron	30.0%
GCTGAGTTAAATAGATTAAC+AGG	-	chr7_4:54147601-54147620	Msa1163080:intron	30.0%
GTATTGAAGTTGCAATCAAA+AGG	-	chr7_4:54147695-54147714	Msa1163080:intron	30.0%
GGTTTATCAATATGCAAGTT+TGG	-	chr7_4:54147932-54147951	Msa1163080:intron	30.0%
TCTTTGCATGATGTATTACA+TGG	-	chr7_4:54147957-54147976	Msa1163080:intron	30.0%
ATGTGAATCTCACATAAAGT+TGG	+	chr7_4:54148079-54148098	None:intergenic	30.0%
ATTGAATAACAAGACATTGC+TGG	+	chr7_4:54148383-54148402	None:intergenic	30.0%
CTGAAGTTTAATGAGCTAAT+TGG	-	chr7_4:54148937-54148956	Msa1163080:CDS	30.0%
ATTTGTACAAACGAGTGAAT+AGG	-	chr7_4:54149240-54149259	Msa1163080:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	54147147	54150087	54147147	ID=Msa1163080;Name=Msa1163080
chr7_4	mRNA	54147147	54150087	54147147	ID=Msa1163080-mRNA-1;Parent=Msa1163080;Name=Msa1163080-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0\|0\|0\|1\|1\|1\|7\|0\|332
chr7_4	exon	54149982	54150087	54149982	ID=Msa1163080-mRNA-1:exon:15711;Parent=Msa1163080-mRNA-1
chr7_4	exon	54149466	54149611	54149466	ID=Msa1163080-mRNA-1:exon:15710;Parent=Msa1163080-mRNA-1
chr7_4	exon	54149256	54149382	54149256	ID=Msa1163080-mRNA-1:exon:15709;Parent=Msa1163080-mRNA-1
chr7_4	exon	54148722	54148995	54148722	ID=Msa1163080-mRNA-1:exon:15708;Parent=Msa1163080-mRNA-1
chr7_4	exon	54148327	54148459	54148327	ID=Msa1163080-mRNA-1:exon:15707;Parent=Msa1163080-mRNA-1
chr7_4	exon	54148126	54148212	54148126	ID=Msa1163080-mRNA-1:exon:15706;Parent=Msa1163080-mRNA-1
chr7_4	exon	54147147	54147272	54147147	ID=Msa1163080-mRNA-1:exon:15705;Parent=Msa1163080-mRNA-1
chr7_4	CDS	54149982	54150087	54149982	ID=Msa1163080-mRNA-1:cds;Parent=Msa1163080-mRNA-1
chr7_4	CDS	54149466	54149611	54149466	ID=Msa1163080-mRNA-1:cds;Parent=Msa1163080-mRNA-1
chr7_4	CDS	54149256	54149382	54149256	ID=Msa1163080-mRNA-1:cds;Parent=Msa1163080-mRNA-1
chr7_4	CDS	54148722	54148995	54148722	ID=Msa1163080-mRNA-1:cds;Parent=Msa1163080-mRNA-1
chr7_4	CDS	54148327	54148459	54148327	ID=Msa1163080-mRNA-1:cds;Parent=Msa1163080-mRNA-1
chr7_4	CDS	54148126	54148212	54148126	ID=Msa1163080-mRNA-1:cds;Parent=Msa1163080-mRNA-1
chr7_4	CDS	54147147	54147272	54147147	ID=Msa1163080-mRNA-1:cds;Parent=Msa1163080-mRNA-1

Gene Sequence

>Msa1163080

ATGTTGTGTTGCGGAGGTGCAGAAGAAGATACCAGCGGTCCACCTTCAAACCAATACGCTGCACCATCAAGAGGGGGTAATGCATATGGTGGCGGCGGCGGTGGAGGAAACTTTGGTTCAAAGGCCTTCATTGGTGAAGGTTCGTATGGAAGGGTTTACTATGCAAAAATGAATGATGGTATTGAAGTTGCAATCAAAAGGCTGGATACAAGTTCTTCGCCTGATTCTGACTCCAACGACTTCGCTGCTCAGTTATCAGTTGTTTCAAGACTCAAGCATGACAATTTTGTAGAGTTGACTGGATATTGTCTAGAAGCAGACAACAGAATTTTGGTTTATCAATATGCAAGTTTGGGTTCTTTGCATGATGTATTACATGGAAGGAAGGGTGTACAAGGTGCCGAACCTGGTCCAGTTTTAAGCTGGAACCAGAGAGCGAAAATTGCATTTGGTGCGGCAAAAGGCCTAGAGTTTCTTCATGAAAAGGTTCAGCCTTCTATAGTTCACCGCGATGTTCGATCCAGCAATGTCTTGTTATTCAATGACTATGAGGCCAAGATTGCTGATTTCAGCTTGACAAATCAGTCTTCAGATACGGCAGCTCGGCTACATTCAACAAGAGTCTTGGGAACATTTGGCTATCATGCTCCAGAGTATGCAATGACAGGGCAGATAACACAAAAAAGTGATGTTTATAGTTTTGGAGTTGTGCTTTTAGAACTCTTGACTGGAAGAAAGCCAGTAGACCACACAATGCCTAAAGGGCAACAAAGTCTTGTGACTTGGGCAACTCCAAGGTTGAGTGAGGACAAAGTGAAACAATGTGTGGATCCTAAGCTAAACAATGACTACCCCCCAAAGGCAATTGCTAAGTTGGCAGCTGTTGCAGCACTGTGTGTGCAATATGAAGCTGATTTCCGGCCAAACATGACAATTGTTGTCAAAGCTCTCCAGCCACTTCTCAATGCAAAACCGGCTGGTCCTGACTCTAATGCTTGA

Protein sequence

>Msa1163080

MLCCGGAEEDTSGPPSNQYAAPSRGGNAYGGGGGGGNFGSKAFIGEGSYGRVYYAKMNDGIEVAIKRLDTSSSPDSDSNDFAAQLSVVSRLKHDNFVELTGYCLEADNRILVYQYASLGSLHDVLHGRKGVQGAEPGPVLSWNQRAKIAFGAAKGLEFLHEKVQPSIVHRDVRSSNVLLFNDYEAKIADFSLTNQSSDTAARLHSTRVLGTFGYHAPEYAMTGQITQKSDVYSFGVVLLELLTGRKPVDHTMPKGQQSLVTWATPRLSEDKVKQCVDPKLNNDYPPKAIAKLAAVAALCVQYEADFRPNMTIVVKALQPLLNAKPAGPDSNA*