Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1165770 | KEH19403.1 | 69.343 | 137 | 34 | 2 | 11 | 147 | 18 | 146 | 5.21e-58 | 189 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1165770 | sp|Q8GX84|C3H1_ARATH | 48.428 | 159 | 32 | 1 | 19 | 127 | 95 | 253 | 2.87e-43 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1165770 | A0A072U5J1 | 69.343 | 137 | 34 | 2 | 11 | 147 | 18 | 146 | 2.49e-58 | 189 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1165770 | TF | C3H |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1165770 | MtrunA17_Chr7g0269781 | 48.947 | 190 | 31 | 2 | 1 | 124 | 51 | 240 | 1.02e-51 | 167 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1165770 | AT1G01350.1 | 48.428 | 159 | 32 | 1 | 19 | 127 | 95 | 253 | 2.92e-44 | 149 |
| Msa1165770 | AT5G06420.1 | 49.045 | 157 | 30 | 1 | 21 | 127 | 132 | 288 | 6.87e-44 | 148 |
| Msa1165770 | AT5G06420.2 | 49.045 | 157 | 30 | 1 | 21 | 127 | 132 | 288 | 6.87e-44 | 148 |
Find 28 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAGGGAAAAGGACTTTC+TGG | 0.207237 | 7_4:-57765274 | Msa1165770:CDS |
| AAAATGAAAGATGTGCTTCT+TGG | 0.227049 | 7_4:+57765635 | None:intergenic |
| GCGCACACTCTTTGCTTTCT+CGG | 0.276894 | 7_4:+57765335 | None:intergenic |
| GACCGAGGCGACTACAATTC+CGG | 0.339363 | 7_4:-57765387 | Msa1165770:CDS |
| TTGAAGTCTTTGCATATGTC+AGG | 0.387439 | 7_4:+57765456 | None:intergenic |
| TGACATATGCAAAGACTTCA+AGG | 0.411330 | 7_4:-57765454 | Msa1165770:CDS |
| ACCGAGGCGACTACAATTCC+GGG | 0.427387 | 7_4:-57765386 | Msa1165770:CDS |
| TAGCAAAGCAACAGCAACTT+TGG | 0.436558 | 7_4:-57765580 | Msa1165770:CDS |
| TGATGAAACTCTAATATAAG+TGG | 0.474441 | 7_4:+57765494 | None:intergenic |
| TGCAAAGACTTCAAGGAGAC+CGG | 0.477457 | 7_4:-57765447 | Msa1165770:CDS |
| GGTGGCAGTTGGACAAAGAA+TGG | 0.496757 | 7_4:-57765365 | Msa1165770:CDS |
| AAAGAGTGTGCGCGCGCTGA+TGG | 0.507062 | 7_4:-57765325 | Msa1165770:CDS |
| TTGTCTGTTTGTTTGTCTGA+AGG | 0.512605 | 7_4:-57765301 | Msa1165770:CDS |
| TTCAAGGAGACCGGGTATTG+TGG | 0.545327 | 7_4:-57765438 | Msa1165770:CDS |
| CTACAATTCCGGGTGGCAGT+TGG | 0.548426 | 7_4:-57765376 | Msa1165770:CDS |
| ACCCGGAATTGTAGTCGCCT+CGG | 0.550971 | 7_4:+57765385 | None:intergenic |
| TTCTTTGTCCAACTGCCACC+CGG | 0.562902 | 7_4:+57765368 | None:intergenic |
| TGCAAGTTTCTTCACGACCG+AGG | 0.564969 | 7_4:-57765402 | Msa1165770:CDS |
| GAGACCGGGTATTGTGGCTA+CGG | 0.578795 | 7_4:-57765432 | Msa1165770:CDS |
| ATCGCCGTAGCCACAATACC+CGG | 0.586901 | 7_4:+57765428 | None:intergenic |
| GAAACTCTAATATAAGTGGA+AGG | 0.596767 | 7_4:+57765498 | None:intergenic |
| ATCCGTGGGTCACACTCACA+CGG | 0.602004 | 7_4:-57765531 | Msa1165770:CDS |
| ACATGTAAATTACAAACACC+CGG | 0.625741 | 7_4:+57765233 | None:intergenic |
| GCAAAGACTTCAAGGAGACC+GGG | 0.633254 | 7_4:-57765446 | Msa1165770:CDS |
| GTGTGAGTGTGACCCACGGA+TGG | 0.640412 | 7_4:+57765533 | None:intergenic |
| ATATAAGTGGAAGGTCTGAG+AGG | 0.681694 | 7_4:+57765507 | None:intergenic |
| GACCGTGTGAGTGTGACCCA+CGG | 0.684896 | 7_4:+57765529 | None:intergenic |
| GAGGCGACTACAATTCCGGG+TGG | 0.758896 | 7_4:-57765383 | Msa1165770:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGATGAAACTCTAATATAAG+TGG | + | chr7_4:57765435-57765454 | None:intergenic | 25.0% |
| TGAGAAAAAGAACATGAAGA+AGG | - | chr7_4:57765250-57765269 | Msa1165770:CDS | 30.0% | |
| AAAATGAAAGATGTGCTTCT+TGG | + | chr7_4:57765294-57765313 | None:intergenic | 30.0% | |
| GAAACTCTAATATAAGTGGA+AGG | + | chr7_4:57765431-57765450 | None:intergenic | 30.0% | |
| TTTGTCTGAAGGAAAAAAGA+AGG | - | chr7_4:57765636-57765655 | Msa1165770:CDS | 30.0% | |
| TTGTCTGAAGGAAAAAAGAA+GGG | - | chr7_4:57765637-57765656 | Msa1165770:CDS | 30.0% | |
| !!! | ATTTTTCAAGTTCCATCCGT+GGG | - | chr7_4:57765381-57765400 | Msa1165770:CDS | 35.0% |
| TTGAAGTCTTTGCATATGTC+AGG | + | chr7_4:57765473-57765492 | None:intergenic | 35.0% | |
| TGACATATGCAAAGACTTCA+AGG | - | chr7_4:57765472-57765491 | Msa1165770:CDS | 35.0% | |
| TTGTCTGTTTGTTTGTCTGA+AGG | - | chr7_4:57765625-57765644 | Msa1165770:CDS | 35.0% | |
| GAAGGAAAAAAGAAGGGAAA+AGG | - | chr7_4:57765643-57765662 | Msa1165770:CDS | 35.0% | |
| !! | GATTTTGCTGACTACAATTC+CGG | - | chr7_4:57765674-57765693 | Msa1165770:CDS | 35.0% |
| !! | ATTTTGCTGACTACAATTCC+GGG | - | chr7_4:57765675-57765694 | Msa1165770:CDS | 35.0% |
| TAGCAAAGCAACAGCAACTT+TGG | - | chr7_4:57765346-57765365 | Msa1165770:CDS | 40.0% | |
| !!! | CATTTTTCAAGTTCCATCCG+TGG | - | chr7_4:57765380-57765399 | Msa1165770:CDS | 40.0% |
| ATATAAGTGGAAGGTCTGAG+AGG | + | chr7_4:57765422-57765441 | None:intergenic | 40.0% | |
| AAGAAGGGAAAAGGACTTTC+TGG | - | chr7_4:57765652-57765671 | Msa1165770:CDS | 40.0% | |
| TGCAAAGACTTCAAGGAGAC+CGG | - | chr7_4:57765479-57765498 | Msa1165770:CDS | 45.0% | |
| GCAAAGACTTCAAGGAGACC+GGG | - | chr7_4:57765480-57765499 | Msa1165770:CDS | 50.0% | |
| ! | TTCAAGGAGACCGGGTATTG+TGG | - | chr7_4:57765488-57765507 | Msa1165770:CDS | 50.0% |
| TGCAAGTTTCTTCACGACCG+AGG | - | chr7_4:57765524-57765543 | Msa1165770:CDS | 50.0% | |
| TTCTTTGTCCAACTGCCACC+CGG | + | chr7_4:57765561-57765580 | None:intergenic | 50.0% | |
| GGTGGCAGTTGGACAAAGAA+TGG | - | chr7_4:57765561-57765580 | Msa1165770:CDS | 50.0% | |
| GCGCACACTCTTTGCTTTCT+CGG | + | chr7_4:57765594-57765613 | None:intergenic | 50.0% | |
| ATCCGTGGGTCACACTCACA+CGG | - | chr7_4:57765395-57765414 | Msa1165770:CDS | 55.0% | |
| ! | GAGACCGGGTATTGTGGCTA+CGG | - | chr7_4:57765494-57765513 | Msa1165770:CDS | 55.0% |
| ATCGCCGTAGCCACAATACC+CGG | + | chr7_4:57765501-57765520 | None:intergenic | 55.0% | |
| GACCGAGGCGACTACAATTC+CGG | - | chr7_4:57765539-57765558 | Msa1165770:CDS | 55.0% | |
| ACCGAGGCGACTACAATTCC+GGG | - | chr7_4:57765540-57765559 | Msa1165770:CDS | 55.0% | |
| ACCCGGAATTGTAGTCGCCT+CGG | + | chr7_4:57765544-57765563 | None:intergenic | 55.0% | |
| CTACAATTCCGGGTGGCAGT+TGG | - | chr7_4:57765550-57765569 | Msa1165770:CDS | 55.0% | |
| GTGTGAGTGTGACCCACGGA+TGG | + | chr7_4:57765396-57765415 | None:intergenic | 60.0% | |
| GACCGTGTGAGTGTGACCCA+CGG | + | chr7_4:57765400-57765419 | None:intergenic | 60.0% | |
| GAGGCGACTACAATTCCGGG+TGG | - | chr7_4:57765543-57765562 | Msa1165770:CDS | 60.0% | |
| ! | AAAGAGTGTGCGCGCGCTGA+TGG | - | chr7_4:57765601-57765620 | Msa1165770:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 57765242 | 57765706 | 57765242 | ID=Msa1165770;Name=Msa1165770 |
| chr7_4 | mRNA | 57765242 | 57765706 | 57765242 | ID=Msa1165770-mRNA-1;Parent=Msa1165770;Name=Msa1165770-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|154 |
| chr7_4 | exon | 57765242 | 57765706 | 57765242 | ID=Msa1165770-mRNA-1:exon:17529;Parent=Msa1165770-mRNA-1 |
| chr7_4 | CDS | 57765242 | 57765706 | 57765242 | ID=Msa1165770-mRNA-1:cds;Parent=Msa1165770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
