Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1165950 | KEH19403.1 | 71.429 | 133 | 34 | 2 | 11 | 143 | 18 | 146 | 3.77e-59 | 191 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1165950 | sp|Q9FNG6|C3H51_ARATH | 50.000 | 160 | 30 | 1 | 18 | 127 | 129 | 288 | 9.83e-45 | 153 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1165950 | A0A072U5J1 | 71.429 | 133 | 34 | 2 | 11 | 143 | 18 | 146 | 1.80e-59 | 191 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1165950 | TF | C3H |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1165950 | MtrunA17_Chr7g0269781 | 51.309 | 191 | 25 | 3 | 1 | 124 | 51 | 240 | 3.84e-55 | 176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1165950 | AT5G06420.1 | 50.000 | 160 | 30 | 1 | 18 | 127 | 129 | 288 | 9.99e-46 | 153 |
| Msa1165950 | AT5G06420.2 | 50.000 | 160 | 30 | 1 | 18 | 127 | 129 | 288 | 9.99e-46 | 153 |
| Msa1165950 | AT1G01350.1 | 49.375 | 160 | 31 | 1 | 18 | 127 | 94 | 253 | 1.15e-44 | 149 |
Find 29 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAGGGAAAAGGACTTTC+TGG | 0.207237 | 7_4:+57992954 | Msa1165950:CDS |
| GCGCACACTCTTTGCTTTCT+CGG | 0.276894 | 7_4:-57992905 | None:intergenic |
| GACCGAGGCGACTACAATTC+CGG | 0.339363 | 7_4:+57992853 | Msa1165950:CDS |
| TGAAGACTCAAAATGAAAGT+TGG | 0.349810 | 7_4:-57992614 | None:intergenic |
| AAAATGAAAGTTGGGCTTCT+CGG | 0.382812 | 7_4:-57992605 | None:intergenic |
| TTGAAGTCTTTGCATATGTC+AGG | 0.387439 | 7_4:-57992784 | None:intergenic |
| GAAGACTCAAAATGAAAGTT+GGG | 0.391037 | 7_4:-57992613 | None:intergenic |
| TGACATATGCAAAGACTTCA+AGG | 0.411330 | 7_4:+57992786 | Msa1165950:CDS |
| ACCGAGGCGACTACAATTCC+GGG | 0.427387 | 7_4:+57992854 | Msa1165950:CDS |
| TAGCAAAGCAACAGCAACTT+TGG | 0.436558 | 7_4:+57992660 | Msa1165950:CDS |
| TGCAAAGACTTCAAGGAGAC+CGG | 0.477457 | 7_4:+57992793 | Msa1165950:CDS |
| GGTGGCAGTTGGACAAAGAA+TGG | 0.496757 | 7_4:+57992875 | Msa1165950:CDS |
| AAAGAGTGTGCGCGCGCTGA+TGG | 0.507062 | 7_4:+57992915 | Msa1165950:CDS |
| TTCAAGGAGACCGGGTATTG+TGG | 0.545327 | 7_4:+57992802 | Msa1165950:CDS |
| CTACAATTCCGGGTGGCAGT+TGG | 0.548426 | 7_4:+57992864 | Msa1165950:CDS |
| ACCCGGAATTGTAGTCGCCT+CGG | 0.550971 | 7_4:-57992855 | None:intergenic |
| CGCGCTGATGGTTTGTCTGA+AGG | 0.556877 | 7_4:+57992927 | Msa1165950:CDS |
| TTCTTTGTCCAACTGCCACC+CGG | 0.562902 | 7_4:-57992872 | None:intergenic |
| TGCAAGTTTCTTCACGACCG+AGG | 0.564969 | 7_4:+57992838 | Msa1165950:CDS |
| GAGACCGGGTATTGTGGCTA+CGG | 0.578795 | 7_4:+57992808 | Msa1165950:CDS |
| ATCGCCGTAGCCACAATACC+CGG | 0.586901 | 7_4:-57992812 | None:intergenic |
| GAAACTCTAATATGAGTCGA+AGG | 0.595574 | 7_4:-57992742 | None:intergenic |
| ACATGTAAATTACAAACACC+CGG | 0.625741 | 7_4:-57992995 | None:intergenic |
| GCAAAGACTTCAAGGAGACC+GGG | 0.633254 | 7_4:+57992794 | Msa1165950:CDS |
| ATCCGTGGGTCATACTCACA+CGG | 0.649413 | 7_4:+57992709 | Msa1165950:CDS |
| GTGTGAGTATGACCCACGGA+TGG | 0.652577 | 7_4:-57992707 | None:intergenic |
| GACCGTGTGAGTATGACCCA+CGG | 0.674066 | 7_4:-57992711 | None:intergenic |
| ATATGAGTCGAAGGTCTGAG+AGG | 0.697705 | 7_4:-57992733 | None:intergenic |
| GAGGCGACTACAATTCCGGG+TGG | 0.758896 | 7_4:+57992857 | Msa1165950:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGAGAAAAAGAACATGAAGA+AGG | + | chr7_4:57992564-57992583 | Msa1165950:CDS | 30.0% | |
| GAAGACTCAAAATGAAAGTT+GGG | - | chr7_4:57992616-57992635 | None:intergenic | 30.0% | |
| TGAAGACTCAAAATGAAAGT+TGG | - | chr7_4:57992617-57992636 | None:intergenic | 30.0% | |
| TTTGTCTGAAGGAAAAAAGA+AGG | + | chr7_4:57992938-57992957 | Msa1165950:CDS | 30.0% | |
| TTGTCTGAAGGAAAAAAGAA+GGG | + | chr7_4:57992939-57992958 | Msa1165950:CDS | 30.0% | |
| AAAATGAAAGTTGGGCTTCT+CGG | - | chr7_4:57992608-57992627 | None:intergenic | 35.0% | |
| !!! | ATTTTTCAAGTTCCATCCGT+GGG | + | chr7_4:57992695-57992714 | Msa1165950:CDS | 35.0% |
| GAAACTCTAATATGAGTCGA+AGG | - | chr7_4:57992745-57992764 | None:intergenic | 35.0% | |
| TTGAAGTCTTTGCATATGTC+AGG | - | chr7_4:57992787-57992806 | None:intergenic | 35.0% | |
| TGACATATGCAAAGACTTCA+AGG | + | chr7_4:57992786-57992805 | Msa1165950:CDS | 35.0% | |
| GAAGGAAAAAAGAAGGGAAA+AGG | + | chr7_4:57992945-57992964 | Msa1165950:CDS | 35.0% | |
| !! | GATTTTGCTGACTACAATTC+CGG | + | chr7_4:57992976-57992995 | Msa1165950:CDS | 35.0% |
| !! | ATTTTGCTGACTACAATTCC+GGG | + | chr7_4:57992977-57992996 | Msa1165950:CDS | 35.0% |
| TAGCAAAGCAACAGCAACTT+TGG | + | chr7_4:57992660-57992679 | Msa1165950:CDS | 40.0% | |
| !!! | CATTTTTCAAGTTCCATCCG+TGG | + | chr7_4:57992694-57992713 | Msa1165950:CDS | 40.0% |
| AAGAAGGGAAAAGGACTTTC+TGG | + | chr7_4:57992954-57992973 | Msa1165950:CDS | 40.0% | |
| ATATGAGTCGAAGGTCTGAG+AGG | - | chr7_4:57992736-57992755 | None:intergenic | 45.0% | |
| TGCAAAGACTTCAAGGAGAC+CGG | + | chr7_4:57992793-57992812 | Msa1165950:CDS | 45.0% | |
| ATCCGTGGGTCATACTCACA+CGG | + | chr7_4:57992709-57992728 | Msa1165950:CDS | 50.0% | |
| GCAAAGACTTCAAGGAGACC+GGG | + | chr7_4:57992794-57992813 | Msa1165950:CDS | 50.0% | |
| ! | TTCAAGGAGACCGGGTATTG+TGG | + | chr7_4:57992802-57992821 | Msa1165950:CDS | 50.0% |
| TGCAAGTTTCTTCACGACCG+AGG | + | chr7_4:57992838-57992857 | Msa1165950:CDS | 50.0% | |
| TTCTTTGTCCAACTGCCACC+CGG | - | chr7_4:57992875-57992894 | None:intergenic | 50.0% | |
| GGTGGCAGTTGGACAAAGAA+TGG | + | chr7_4:57992875-57992894 | Msa1165950:CDS | 50.0% | |
| GCGCACACTCTTTGCTTTCT+CGG | - | chr7_4:57992908-57992927 | None:intergenic | 50.0% | |
| GTGTGAGTATGACCCACGGA+TGG | - | chr7_4:57992710-57992729 | None:intergenic | 55.0% | |
| GACCGTGTGAGTATGACCCA+CGG | - | chr7_4:57992714-57992733 | None:intergenic | 55.0% | |
| ! | GAGACCGGGTATTGTGGCTA+CGG | + | chr7_4:57992808-57992827 | Msa1165950:CDS | 55.0% |
| ATCGCCGTAGCCACAATACC+CGG | - | chr7_4:57992815-57992834 | None:intergenic | 55.0% | |
| GACCGAGGCGACTACAATTC+CGG | + | chr7_4:57992853-57992872 | Msa1165950:CDS | 55.0% | |
| ACCGAGGCGACTACAATTCC+GGG | + | chr7_4:57992854-57992873 | Msa1165950:CDS | 55.0% | |
| ACCCGGAATTGTAGTCGCCT+CGG | - | chr7_4:57992858-57992877 | None:intergenic | 55.0% | |
| CTACAATTCCGGGTGGCAGT+TGG | + | chr7_4:57992864-57992883 | Msa1165950:CDS | 55.0% | |
| ! | CGCGCTGATGGTTTGTCTGA+AGG | + | chr7_4:57992927-57992946 | Msa1165950:CDS | 55.0% |
| GAGGCGACTACAATTCCGGG+TGG | + | chr7_4:57992857-57992876 | Msa1165950:CDS | 60.0% | |
| ! | AAAGAGTGTGCGCGCGCTGA+TGG | + | chr7_4:57992915-57992934 | Msa1165950:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 57992556 | 57993008 | 57992556 | ID=Msa1165950;Name=Msa1165950 |
| chr7_4 | mRNA | 57992556 | 57993008 | 57992556 | ID=Msa1165950-mRNA-1;Parent=Msa1165950;Name=Msa1165950-mRNA-1;_AED=0.45;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|150 |
| chr7_4 | exon | 57992556 | 57993008 | 57992556 | ID=Msa1165950-mRNA-1:exon:17686;Parent=Msa1165950-mRNA-1 |
| chr7_4 | CDS | 57992556 | 57993008 | 57992556 | ID=Msa1165950-mRNA-1:cds;Parent=Msa1165950-mRNA-1 |
| Gene Sequence |
| Protein sequence |
