AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1167790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1167790 MtrunA17_Chr7g0265291 100.000 206 0 0 1 206 1 206 9.32e-156 429
Msa1167790 MtrunA17_Chr1g0179781 78.922 204 40 2 1 203 1 202 3.05e-115 326
Msa1167790 MtrunA17_Chr5g0395181 56.098 205 81 4 2 206 16 211 1.16e-76 229
Msa1167790 MtrunA17_Chr5g0411151 57.767 206 69 5 7 203 35 231 1.41e-75 227
Msa1167790 MtrunA17_Chr4g0040071 55.392 204 75 6 7 203 15 209 6.31e-74 222
Msa1167790 MtrunA17_Chr8g0366581 58.333 204 71 5 7 203 33 229 2.94e-73 221
Msa1167790 MtrunA17_Chr2g0320521 40.249 241 95 11 1 202 1 231 1.73e-44 148
Msa1167790 MtrunA17_Chr4g0020811 51.034 145 59 6 1 136 1 142 9.88e-40 133
Msa1167790 MtrunA17_Chr2g0308401 49.296 142 61 5 4 136 1 140 8.78e-39 130
Msa1167790 MtrunA17_Chr2g0297251 35.242 227 112 5 7 203 37 258 1.87e-36 128
Msa1167790 MtrunA17_Chr2g0320561 47.651 149 67 4 4 143 1 147 4.48e-36 126
Msa1167790 MtrunA17_Chr2g0320551 47.183 142 64 4 4 136 1 140 1.80e-35 122
Msa1167790 MtrunA17_Chr7g0230511 42.754 138 74 3 5 142 7 139 8.83e-35 123
Msa1167790 MtrunA17_Chr4g0065041 42.647 136 74 1 7 142 8 139 2.68e-33 119
Msa1167790 MtrunA17_Chr8g0386511 33.333 159 93 4 48 202 4 153 4.93e-21 85.1
Msa1167790 MtrunA17_Chr8g0392151 34.615 156 76 6 9 143 15 165 3.98e-18 79.7
Msa1167790 MtrunA17_Chr4g0052451 43.902 82 40 1 61 136 10 91 8.57e-18 75.1
Msa1167790 MtrunA17_Chr5g0434331 27.363 201 110 6 4 202 40 206 1.48e-16 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1167790 AT1G32700.1 55.941 202 78 5 7 203 12 207 2.23e-71 216
Msa1167790 AT4G17900.1 53.659 205 76 6 7 203 34 227 1.06e-67 207
Msa1167790 AT4G17900.2 53.659 205 76 6 7 203 20 213 1.06e-67 207
Msa1167790 AT2G27930.1 55.072 207 63 4 7 204 4 189 4.61e-67 204
Msa1167790 AT2G27930.4 55.072 207 63 4 7 204 4 189 4.61e-67 204
Msa1167790 AT1G76590.1 48.918 231 80 6 7 202 18 245 6.97e-67 206
Msa1167790 AT1G21000.2 49.362 235 76 10 7 202 13 243 2.03e-66 204
Msa1167790 AT1G21000.1 49.362 235 76 10 7 202 16 246 2.93e-66 204
Msa1167790 AT1G43000.2 46.154 221 91 7 2 202 11 223 3.40e-62 193
Msa1167790 AT1G43000.1 46.154 221 91 7 2 202 4 216 4.62e-62 192
Msa1167790 AT2G27930.3 66.187 139 36 2 7 136 4 140 2.09e-59 184
Msa1167790 AT2G27930.2 66.187 139 36 2 7 136 4 140 2.09e-59 184
Msa1167790 AT1G32700.2 56.322 174 65 5 35 203 1 168 2.85e-57 179
Msa1167790 AT5G46710.3 48.259 201 96 5 7 202 12 209 3.17e-55 175
Msa1167790 AT5G46710.1 48.259 201 96 5 7 202 28 225 5.03e-55 175
Msa1167790 AT5G46710.2 57.252 131 52 3 7 136 28 155 1.34e-46 151
Msa1167790 AT2G27930.5 52.318 151 44 3 63 204 61 192 7.45e-40 135
Msa1167790 AT2G27930.6 52.318 151 44 3 63 204 61 192 7.45e-40 135
Msa1167790 AT3G60670.1 36.979 192 104 5 1 179 1 188 9.65e-34 121
Msa1167790 AT1G31040.1 34.348 230 111 7 7 202 19 242 3.46e-33 119
Msa1167790 AT2G12646.3 43.796 137 73 2 5 141 1 133 8.60e-33 119
Msa1167790 AT2G12646.1 44.444 135 71 2 7 141 6 136 9.72e-33 119
Msa1167790 AT2G12646.2 44.444 135 71 2 7 141 6 136 9.72e-33 119
Msa1167790 AT2G01818.1 31.905 210 126 4 9 202 13 221 5.57e-26 100
Msa1167790 AT2G01818.2 30.667 150 87 4 69 202 9 157 3.65e-12 62.4

Find 86 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATTTCGGTTCTGTTCTT+TGG 0.144073 7_4:+60256073 Msa1167790:CDS
GCTACTCCTTTCCCAGATTT+TGG 0.169958 7_4:-60256014 None:intergenic
TTTCTTCTCCTTGCATTTAA+AGG 0.172328 7_4:-60257248 None:intergenic
AAAAGAGAGTGTAGTGTAAT+AGG 0.211550 7_4:-60255136 None:intergenic
GAATACAATACAACCCTATT+TGG 0.214365 7_4:+60257368 Msa1167790:three_prime_UTR
ATTCCTTCTAAACTCAAAAT+TGG 0.257186 7_4:+60257315 Msa1167790:three_prime_UTR
GTCCAAGGTACTTCTTGATT+TGG 0.284024 7_4:+60257470 Msa1167790:three_prime_UTR
TGGCTCAATGCGGTGGGATT+TGG 0.304487 7_4:-60255280 None:intergenic
TCATTTCGGTTCTGTTCTTT+GGG 0.305955 7_4:+60256074 Msa1167790:CDS
GGATTCAATGGACGGAGATT+TGG 0.315477 7_4:-60255259 None:intergenic
TTACTACCTGAATGACTTGA+TGG 0.329584 7_4:-60255598 None:intergenic
TAGTGTAATAGGGACGAAAA+AGG 0.345244 7_4:-60255125 None:intergenic
GTTAGGCCTCAACCAAAATC+TGG 0.351742 7_4:+60256002 Msa1167790:CDS
CAAAGACCATCAAGTCATTC+AGG 0.358735 7_4:+60255592 Msa1167790:CDS
GTCGTTGAATTCTAAGTTGA+AGG 0.367643 7_4:+60255169 Msa1167790:five_prime_UTR
GAAGAAAAGGTATACCTCAT+AGG 0.379000 7_4:+60257264 Msa1167790:CDS
ATACCTCATAGGGCACCTTT+TGG 0.387290 7_4:+60257275 Msa1167790:CDS
GTTCTGTTCTTTGGGTTGTA+AGG 0.391861 7_4:+60256082 Msa1167790:CDS
TGGAGATGCAAGCTTTGCTT+TGG 0.396373 7_4:+60257115 Msa1167790:CDS
GGAGCTTGTTGGATTCATTT+CGG 0.401131 7_4:+60256060 Msa1167790:CDS
ACACAGTGTTGGTGCCTCCT+TGG 0.407393 7_4:+60255429 Msa1167790:CDS
CTCCTTGCATTTAAAGGAGG+TGG 0.416107 7_4:-60257242 None:intergenic
CAGAAAGTGTTGGACATTAG+TGG 0.416506 7_4:+60255933 Msa1167790:CDS
AAGTAAAGGAAAGGGTATAT+AGG 0.426584 7_4:+60257495 Msa1167790:three_prime_UTR
GAGATTGAAAGTGGTGGCTG+AGG 0.430293 7_4:-60255065 None:intergenic
TATTAAATTAAAGAAGTAAA+GGG 0.434227 7_4:-60255099 None:intergenic
AAAGAGAGTGTAGTGTAATA+GGG 0.435032 7_4:-60255135 None:intergenic
GCGGTGGGATTTGGATTCAA+TGG 0.442150 7_4:-60255271 None:intergenic
GGAGGTGGAGGAGGCGTGAG+AGG 0.445087 7_4:-60257227 None:intergenic
GCTCACATTTGTGAGATTTG+TGG 0.456800 7_4:+60256035 Msa1167790:CDS
AGATGCAAGCTTTGCTTTGG+AGG 0.464996 7_4:+60257118 Msa1167790:CDS
GCTTTGGAGGGTAAGAATGA+AGG 0.469335 7_4:+60257131 Msa1167790:CDS
TTAAATGCAAGGAGAAGAAA+AGG 0.487088 7_4:+60257251 Msa1167790:CDS
TTCTTGATTTGGAAAAGTAA+AGG 0.488767 7_4:+60257481 Msa1167790:three_prime_UTR
TTCAACAATGGTTCAAGCCA+AGG 0.490118 7_4:-60255446 None:intergenic
TTATTAAATTAAAGAAGTAA+AGG 0.491405 7_4:-60255100 None:intergenic
GCATTTAAAGGAGGTGGAGG+AGG 0.494494 7_4:-60257236 None:intergenic
CTTCTCCTTGCATTTAAAGG+AGG 0.503493 7_4:-60257245 None:intergenic
TACAATACAACCCTATTTGG+TGG 0.509396 7_4:+60257371 Msa1167790:three_prime_UTR
TTCCAAATCAAGAAGTACCT+TGG 0.510498 7_4:-60257472 None:intergenic
ATTTGGAAAAGTAAAGGAAA+GGG 0.516123 7_4:+60257487 Msa1167790:three_prime_UTR
AATGAAGGATTGACAATGGA+AGG 0.518953 7_4:+60257146 Msa1167790:CDS
AAGAAAAGGTATACCTCATA+GGG 0.519252 7_4:+60257265 Msa1167790:CDS
ATTGAAAGTGGTGGCTGAGG+GGG 0.521162 7_4:-60255062 None:intergenic
ACAATACAACCCTATTTGGT+GGG 0.523862 7_4:+60257372 Msa1167790:three_prime_UTR
GATGCAAGCTTTGCTTTGGA+GGG 0.525049 7_4:+60257119 Msa1167790:CDS
TAGGGTTGTATTGTATTCCA+AGG 0.525651 7_4:-60257363 None:intergenic
GAATTATGGACGTTGGATGA+AGG 0.525828 7_4:-60255040 None:intergenic
GATTTGTGGAAGGAGCTTGT+TGG 0.527361 7_4:+60256049 Msa1167790:CDS
GGGACCCAAACCGGAAAAGA+AGG 0.530188 7_4:-60257528 None:intergenic
AGATTGAAAGTGGTGGCTGA+GGG 0.531242 7_4:-60255064 None:intergenic
GAAGGAATCCCATTAGAATG+AGG 0.534651 7_4:-60257300 None:intergenic
AGATTAGGAGCCTTTGGAGG+GGG 0.542302 7_4:+60257443 Msa1167790:three_prime_UTR
CTCCACCTCCTTTAAATGCA+AGG 0.542768 7_4:+60257240 Msa1167790:CDS
AAAAGATTAGGAGCCTTTGG+AGG 0.548418 7_4:+60257440 Msa1167790:three_prime_UTR
CTTGCATTTAAAGGAGGTGG+AGG 0.548902 7_4:-60257239 None:intergenic
AATTAAAGAAGTAAAGGGAA+TGG 0.552187 7_4:-60255094 None:intergenic
TTAGGCCTCAACCAAAATCT+GGG 0.556600 7_4:+60256003 Msa1167790:CDS
CATTAGAATGAGGAACCAAA+AGG 0.564563 7_4:-60257290 None:intergenic
GATTTGGAAAAGTAAAGGAA+AGG 0.564681 7_4:+60257486 Msa1167790:three_prime_UTR
AAAGATTAGGAGCCTTTGGA+GGG 0.565639 7_4:+60257441 Msa1167790:three_prime_UTR
TGGGATTTGGATTCAATGGA+CGG 0.571190 7_4:-60255267 None:intergenic
GAACCAAAAGGTGCCCTATG+AGG 0.572485 7_4:-60257278 None:intergenic
TAAGAATGAAGGATTGACAA+TGG 0.573703 7_4:+60257142 Msa1167790:CDS
TGGACGGATGAGATTGAAAG+TGG 0.574045 7_4:-60255074 None:intergenic
AAAAGAATTATATAATGACT+AGG 0.588212 7_4:-60257400 None:intergenic
GTCAGAGATTCAGAAAGTGT+TGG 0.588527 7_4:+60255923 Msa1167790:CDS
TATTGCTTAGATTGCAACAA+TGG 0.589105 7_4:+60255530 Msa1167790:CDS
GATAATGTGGCTCAATGCGG+TGG 0.592110 7_4:-60255287 None:intergenic
AAAGAAGTAAAGGGAATGGA+CGG 0.596085 7_4:-60255090 None:intergenic
AGGGTTGTATTGTATTCCAA+GGG 0.597466 7_4:-60257362 None:intergenic
CCTCAACCAAAATCTGGGAA+AGG 0.603599 7_4:+60256008 Msa1167790:CDS
TACCATTGTGGTTGATAATG+TGG 0.604405 7_4:-60255300 None:intergenic
TAAATACTTAAATACCATTG+TGG 0.605312 7_4:-60255312 None:intergenic
ACATTTGTGAGATTTGTGGA+AGG 0.623549 7_4:+60256039 Msa1167790:CDS
CAATGGACGGAGATTTGGAG+AGG 0.625790 7_4:-60255254 None:intergenic
AACAATGGTTCAAGCCAAGG+AGG 0.647379 7_4:-60255443 None:intergenic
TTAGATTGCAACAATGGTGA+TGG 0.649321 7_4:+60255536 Msa1167790:CDS
TTTGAAACAGGACACAGTGT+TGG 0.663854 7_4:+60255418 Msa1167790:intron
TCAGAAGAAGAATTGCGTGA+AGG 0.679765 7_4:+60257185 Msa1167790:CDS
GATTGAAAGTGGTGGCTGAG+GGG 0.679935 7_4:-60255063 None:intergenic
ATAATGTGGCTCAATGCGGT+GGG 0.680644 7_4:-60255286 None:intergenic
AAGATTAGGAGCCTTTGGAG+GGG 0.685066 7_4:+60257442 Msa1167790:three_prime_UTR
AGCCACATTATCAACCACAA+TGG 0.705584 7_4:+60255298 Msa1167790:exon
ACGGATGAGATTGAAAGTGG+TGG 0.723086 7_4:-60255071 None:intergenic
GTTGATAATGTGGCTCAATG+CGG 0.757064 7_4:-60255290 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAATTAAAGAAGTAAA+GGG - chr7_4:60255102-60255121 None:intergenic 10.0%
!! TTATTAAATTAAAGAAGTAA+AGG - chr7_4:60255103-60255122 None:intergenic 10.0%
!!! TAATAATATTCTCTTTTTTA+CGG + chr7_4:60256415-60256434 Msa1167790:intron 10.0%
!! TTAAACTATATTTAGATAAA+GGG - chr7_4:60256761-60256780 None:intergenic 10.0%
!! ATTAAACTATATTTAGATAA+AGG - chr7_4:60256762-60256781 None:intergenic 10.0%
!!! AATTCTTTTTACAATAATAA+AGG + chr7_4:60257414-60257433 Msa1167790:three_prime_UTR 10.0%
!!! TTTTTTTCTTCATTTTTTTG+TGG + chr7_4:60255343-60255362 Msa1167790:intron 15.0%
!!! TTTGATGTTTTTAAGAATAA+TGG + chr7_4:60255387-60255406 Msa1167790:intron 15.0%
!!! TTGATGTTTTTAAGAATAAT+GGG + chr7_4:60255388-60255407 Msa1167790:intron 15.0%
!! TAAACATTCATATATGAAAT+GGG - chr7_4:60255688-60255707 None:intergenic 15.0%
!! TTAAACATTCATATATGAAA+TGG - chr7_4:60255689-60255708 None:intergenic 15.0%
!!! TATATGAATGTTTAATTTTC+TGG + chr7_4:60255694-60255713 Msa1167790:intron 15.0%
!!! ATATGAATGTTTAATTTTCT+GGG + chr7_4:60255695-60255714 Msa1167790:intron 15.0%
!!! TATTATGAGTTTTATAAACA+TGG + chr7_4:60256448-60256467 Msa1167790:intron 15.0%
!!! ATTATGAGTTTTATAAACAT+GGG + chr7_4:60256449-60256468 Msa1167790:intron 15.0%
!! TTTGTTTATCTATCATAATT+AGG + chr7_4:60256515-60256534 Msa1167790:intron 15.0%
!!! TGATAATGTACAAAATTTAT+TGG - chr7_4:60256605-60256624 None:intergenic 15.0%
!! GATAATATATAATATTTGCT+TGG + chr7_4:60256695-60256714 Msa1167790:intron 15.0%
!! AAAAGAATTATATAATGACT+AGG - chr7_4:60257403-60257422 None:intergenic 15.0%
!! TAAATACTTAAATACCATTG+TGG - chr7_4:60255315-60255334 None:intergenic 20.0%
!! TTTCTTTATTGAAAACCTAA+CGG + chr7_4:60255627-60255646 Msa1167790:intron 20.0%
!!! TATTAATGTATTTGGTGAAA+TGG + chr7_4:60255730-60255749 Msa1167790:intron 20.0%
!!! ATTAATGTATTTGGTGAAAT+GGG + chr7_4:60255731-60255750 Msa1167790:intron 20.0%
!! TTGATGTATAATGTAATGTA+AGG + chr7_4:60255773-60255792 Msa1167790:intron 20.0%
!! TGATGTATAATGTAATGTAA+GGG + chr7_4:60255774-60255793 Msa1167790:intron 20.0%
!! ATAATTATGATGTTGAATGA+TGG - chr7_4:60256299-60256318 None:intergenic 20.0%
!!! TTATGAGTTTTATAAACATG+GGG + chr7_4:60256450-60256469 Msa1167790:intron 20.0%
!! AAAAAGAAAACTCAAAATGT+AGG - chr7_4:60256490-60256509 None:intergenic 20.0%
!!! TGAAAAATAATATGATGATG+TGG + chr7_4:60256673-60256692 Msa1167790:intron 20.0%
!! GTAAAAATGATTTACAAAGT+CGG + chr7_4:60256726-60256745 Msa1167790:intron 20.0%
!!! ATTTAATTTATATACCACGT+TGG - chr7_4:60256950-60256969 None:intergenic 20.0%
!! CTTGAAAGAATAAAGAAAAA+TGG + chr7_4:60257095-60257114 Msa1167790:CDS 20.0%
!! TAATAAAGGTGAAAAAGATT+AGG + chr7_4:60257428-60257447 Msa1167790:three_prime_UTR 20.0%
! AATTAAAGAAGTAAAGGGAA+TGG - chr7_4:60255097-60255116 None:intergenic 25.0%
!!! CATTTTTTTGTGGTATACTA+TGG + chr7_4:60255353-60255372 Msa1167790:intron 25.0%
!!! GATTTGTTTGTTGTTTTTGT+TGG + chr7_4:60255857-60255876 Msa1167790:intron 25.0%
! ACTCAATTATACTCATCATA+TGG + chr7_4:60256223-60256242 Msa1167790:intron 25.0%
! CTCAATTATACTCATCATAT+GGG + chr7_4:60256224-60256243 Msa1167790:intron 25.0%
! GCATGATGAATAATTAAAAG+CGG - chr7_4:60256329-60256348 None:intergenic 25.0%
! GGTAAATGTACTAATTATGA+AGG - chr7_4:60256351-60256370 None:intergenic 25.0%
! GTAATTAATCGAAATGTGAA+TGG - chr7_4:60256372-60256391 None:intergenic 25.0%
! TCTATCATAATTAGGACATT+TGG + chr7_4:60256523-60256542 Msa1167790:intron 25.0%
! GCATGTCATATACAAATAAT+TGG + chr7_4:60257019-60257038 Msa1167790:intron 25.0%
! ATTCCTTCTAAACTCAAAAT+TGG + chr7_4:60257315-60257334 Msa1167790:three_prime_UTR 25.0%
!!! CAATATTCAATTTTTTCCCT+TGG + chr7_4:60257346-60257365 Msa1167790:three_prime_UTR 25.0%
!!! TTCTTGATTTGGAAAAGTAA+AGG + chr7_4:60257481-60257500 Msa1167790:three_prime_UTR 25.0%
! ATTTGGAAAAGTAAAGGAAA+GGG + chr7_4:60257487-60257506 Msa1167790:three_prime_UTR 25.0%
! AAAGAGAGTGTAGTGTAATA+GGG - chr7_4:60255138-60255157 None:intergenic 30.0%
! AAAAGAGAGTGTAGTGTAAT+AGG - chr7_4:60255139-60255158 None:intergenic 30.0%
! AAAAACGGTGTTTTCAACAA+TGG - chr7_4:60255461-60255480 None:intergenic 30.0%
ATCCGACAAATGTTGAAAAA+CGG - chr7_4:60255476-60255495 None:intergenic 30.0%
TATTGCTTAGATTGCAACAA+TGG + chr7_4:60255530-60255549 Msa1167790:CDS 30.0%
AAAACCTAACGGCAAAATAT+AGG + chr7_4:60255638-60255657 Msa1167790:intron 30.0%
GCATTGCAGATTCAAAAAAT+GGG + chr7_4:60255660-60255679 Msa1167790:intron 30.0%
TGTATAATGTAATGTAAGGG+TGG + chr7_4:60255777-60255796 Msa1167790:intron 30.0%
!!! GAGTTTTGTTCTTGAATGTT+AGG + chr7_4:60255985-60256004 Msa1167790:CDS 30.0%
!! GTTGATAATGATATGGACAT+TGG - chr7_4:60256187-60256206 None:intergenic 30.0%
!! TGTGCATGTTGATAATGATA+TGG - chr7_4:60256194-60256213 None:intergenic 30.0%
! AAATGTAGGTGATGATGTAT+GGG - chr7_4:60256264-60256283 None:intergenic 30.0%
GATGAATAATTAAAAGCGGT+GGG - chr7_4:60256325-60256344 None:intergenic 30.0%
TGATGAATAATTAAAAGCGG+TGG - chr7_4:60256326-60256345 None:intergenic 30.0%
AAAATCACAATGCGTCAATT+AGG - chr7_4:60256552-60256571 None:intergenic 30.0%
CAGAAACTATGAGATTTGAT+TGG - chr7_4:60256629-60256648 None:intergenic 30.0%
!! TGGTGTAAAGGCTTTTAAAA+CGG - chr7_4:60256930-60256949 None:intergenic 30.0%
TAAGAATGAAGGATTGACAA+TGG + chr7_4:60257142-60257161 Msa1167790:CDS 30.0%
TTTCTTCTCCTTGCATTTAA+AGG - chr7_4:60257251-60257270 None:intergenic 30.0%
TTAAATGCAAGGAGAAGAAA+AGG + chr7_4:60257251-60257270 Msa1167790:CDS 30.0%
! AAGAAAAGGTATACCTCATA+GGG + chr7_4:60257265-60257284 Msa1167790:CDS 30.0%
!!! TTTTGGTTCCTCATTCTAAT+GGG + chr7_4:60257292-60257311 Msa1167790:exon 30.0%
!!! GTACCAATTTTGAGTTTAGA+AGG - chr7_4:60257321-60257340 None:intergenic 30.0%
GAATACAATACAACCCTATT+TGG + chr7_4:60257368-60257387 Msa1167790:three_prime_UTR 30.0%
GATTTGGAAAAGTAAAGGAA+AGG + chr7_4:60257486-60257505 Msa1167790:three_prime_UTR 30.0%
! AAGTAAAGGAAAGGGTATAT+AGG + chr7_4:60257495-60257514 Msa1167790:three_prime_UTR 30.0%
! AAAGAAAACTCCTTCTTTTC+CGG + chr7_4:60257518-60257537 Msa1167790:three_prime_UTR 30.0%
AAAGAAGTAAAGGGAATGGA+CGG - chr7_4:60255093-60255112 None:intergenic 35.0%
AGTGTAATAGGGACGAAAAA+GGG - chr7_4:60255127-60255146 None:intergenic 35.0%
TAGTGTAATAGGGACGAAAA+AGG - chr7_4:60255128-60255147 None:intergenic 35.0%
! GTCGTTGAATTCTAAGTTGA+AGG + chr7_4:60255169-60255188 Msa1167790:five_prime_UTR 35.0%
!! TACCATTGTGGTTGATAATG+TGG - chr7_4:60255303-60255322 None:intergenic 35.0%
! ATGGGTCAAATGTTTGAAAC+AGG + chr7_4:60255406-60255425 Msa1167790:intron 35.0%
!! CACCGTTTTTCAACATTTGT+CGG + chr7_4:60255471-60255490 Msa1167790:CDS 35.0%
TTAGATTGCAACAATGGTGA+TGG + chr7_4:60255536-60255555 Msa1167790:CDS 35.0%
TTACTACCTGAATGACTTGA+TGG - chr7_4:60255601-60255620 None:intergenic 35.0%
! AATGCCTATATTTTGCCGTT+AGG - chr7_4:60255645-60255664 None:intergenic 35.0%
GGCATTGCAGATTCAAAAAA+TGG + chr7_4:60255659-60255678 Msa1167790:intron 35.0%
ACATTTGTGAGATTTGTGGA+AGG + chr7_4:60256039-60256058 Msa1167790:CDS 35.0%
TTCATTTCGGTTCTGTTCTT+TGG + chr7_4:60256073-60256092 Msa1167790:CDS 35.0%
TCATTTCGGTTCTGTTCTTT+GGG + chr7_4:60256074-60256093 Msa1167790:CDS 35.0%
! CAAATGTAGGTGATGATGTA+TGG - chr7_4:60256265-60256284 None:intergenic 35.0%
! TGATGTTGAATGATGGAGAT+GGG - chr7_4:60256292-60256311 None:intergenic 35.0%
! ATGATGTTGAATGATGGAGA+TGG - chr7_4:60256293-60256312 None:intergenic 35.0%
TATATACCACGTTGGTGTAA+AGG - chr7_4:60256942-60256961 None:intergenic 35.0%
! ATATATTCTGCTGCATTGAC+AGG + chr7_4:60256988-60257007 Msa1167790:intron 35.0%
! TATATTCTGCTGCATTGACA+GGG + chr7_4:60256989-60257008 Msa1167790:intron 35.0%
AATGAAGGATTGACAATGGA+AGG + chr7_4:60257146-60257165 Msa1167790:CDS 35.0%
! GAAGAAAAGGTATACCTCAT+AGG + chr7_4:60257264-60257283 Msa1167790:CDS 35.0%
CATTAGAATGAGGAACCAAA+AGG - chr7_4:60257293-60257312 None:intergenic 35.0%
!!! CTTTTGGTTCCTCATTCTAA+TGG + chr7_4:60257291-60257310 Msa1167790:CDS 35.0%
AGGGTTGTATTGTATTCCAA+GGG - chr7_4:60257365-60257384 None:intergenic 35.0%
TAGGGTTGTATTGTATTCCA+AGG - chr7_4:60257366-60257385 None:intergenic 35.0%
TACAATACAACCCTATTTGG+TGG + chr7_4:60257371-60257390 Msa1167790:three_prime_UTR 35.0%
ACAATACAACCCTATTTGGT+GGG + chr7_4:60257372-60257391 Msa1167790:three_prime_UTR 35.0%
! TAGGTTTTTCCCACCAAATA+GGG - chr7_4:60257384-60257403 None:intergenic 35.0%
TGAAAAAGATTAGGAGCCTT+TGG + chr7_4:60257437-60257456 Msa1167790:three_prime_UTR 35.0%
!! TTCCAAATCAAGAAGTACCT+TGG - chr7_4:60257475-60257494 None:intergenic 35.0%
!!! TTTTTTTTAGGGACCCAAAC+CGG - chr7_4:60257540-60257559 None:intergenic 35.0%
GAATTATGGACGTTGGATGA+AGG - chr7_4:60255043-60255062 None:intergenic 40.0%
GTGTAATAGGGACGAAAAAG+GGG - chr7_4:60255126-60255145 None:intergenic 40.0%
AGAGAGAGAGAGAGAGTAAA+CGG - chr7_4:60255195-60255214 None:intergenic 40.0%
TGGGATTTGGATTCAATGGA+CGG - chr7_4:60255270-60255289 None:intergenic 40.0%
!! GTTGATAATGTGGCTCAATG+CGG - chr7_4:60255293-60255312 None:intergenic 40.0%
AGCCACATTATCAACCACAA+TGG + chr7_4:60255298-60255317 Msa1167790:exon 40.0%
! TTTGAAACAGGACACAGTGT+TGG + chr7_4:60255418-60255437 Msa1167790:intron 40.0%
TTCAACAATGGTTCAAGCCA+AGG - chr7_4:60255449-60255468 None:intergenic 40.0%
CAAAGACCATCAAGTCATTC+AGG + chr7_4:60255592-60255611 Msa1167790:CDS 40.0%
! GTCAGAGATTCAGAAAGTGT+TGG + chr7_4:60255923-60255942 Msa1167790:CDS 40.0%
!! CAGAAAGTGTTGGACATTAG+TGG + chr7_4:60255933-60255952 Msa1167790:CDS 40.0%
TTAGGCCTCAACCAAAATCT+GGG + chr7_4:60256003-60256022 Msa1167790:CDS 40.0%
GCTCACATTTGTGAGATTTG+TGG + chr7_4:60256035-60256054 Msa1167790:CDS 40.0%
!! GGAGCTTGTTGGATTCATTT+CGG + chr7_4:60256060-60256079 Msa1167790:CDS 40.0%
!! GTTCTGTTCTTTGGGTTGTA+AGG + chr7_4:60256082-60256101 Msa1167790:CDS 40.0%
TAAAAGCCTTTACACCAACG+TGG + chr7_4:60256933-60256952 Msa1167790:intron 40.0%
! ATATTCTGCTGCATTGACAG+GGG + chr7_4:60256990-60257009 Msa1167790:intron 40.0%
TCAGAAGAAGAATTGCGTGA+AGG + chr7_4:60257185-60257204 Msa1167790:CDS 40.0%
CTTCTCCTTGCATTTAAAGG+AGG - chr7_4:60257248-60257267 None:intergenic 40.0%
GAAGGAATCCCATTAGAATG+AGG - chr7_4:60257303-60257322 None:intergenic 40.0%
! CTAGGTTTTTCCCACCAAAT+AGG - chr7_4:60257385-60257404 None:intergenic 40.0%
AAAAGATTAGGAGCCTTTGG+AGG + chr7_4:60257440-60257459 Msa1167790:three_prime_UTR 40.0%
AAAGATTAGGAGCCTTTGGA+GGG + chr7_4:60257441-60257460 Msa1167790:three_prime_UTR 40.0%
! GTCCAAGGTACTTCTTGATT+TGG + chr7_4:60257470-60257489 Msa1167790:three_prime_UTR 40.0%
! AAACTCCTTCTTTTCCGGTT+TGG + chr7_4:60257523-60257542 Msa1167790:three_prime_UTR 40.0%
! AACTCCTTCTTTTCCGGTTT+GGG + chr7_4:60257524-60257543 Msa1167790:three_prime_UTR 40.0%
AGATTGAAAGTGGTGGCTGA+GGG - chr7_4:60255067-60255086 None:intergenic 45.0%
ACGGATGAGATTGAAAGTGG+TGG - chr7_4:60255074-60255093 None:intergenic 45.0%
TGGACGGATGAGATTGAAAG+TGG - chr7_4:60255077-60255096 None:intergenic 45.0%
GGATTCAATGGACGGAGATT+TGG - chr7_4:60255262-60255281 None:intergenic 45.0%
ATAATGTGGCTCAATGCGGT+GGG - chr7_4:60255289-60255308 None:intergenic 45.0%
AACAATGGTTCAAGCCAAGG+AGG - chr7_4:60255446-60255465 None:intergenic 45.0%
GTTAGGCCTCAACCAAAATC+TGG + chr7_4:60256002-60256021 Msa1167790:CDS 45.0%
!!! CCTTTCCCAGATTTTGGTTG+AGG - chr7_4:60256011-60256030 None:intergenic 45.0%
CCTCAACCAAAATCTGGGAA+AGG + chr7_4:60256008-60256027 Msa1167790:CDS 45.0%
! GCTACTCCTTTCCCAGATTT+TGG - chr7_4:60256017-60256036 None:intergenic 45.0%
GATTTGTGGAAGGAGCTTGT+TGG + chr7_4:60256049-60256068 Msa1167790:CDS 45.0%
! TGTAGGTGATGATGTATGGG+TGG - chr7_4:60256261-60256280 None:intergenic 45.0%
! GGAGATGGGCTTTCAAATGT+AGG - chr7_4:60256278-60256297 None:intergenic 45.0%
TGGAGATGCAAGCTTTGCTT+TGG + chr7_4:60257115-60257134 Msa1167790:CDS 45.0%
AGATGCAAGCTTTGCTTTGG+AGG + chr7_4:60257118-60257137 Msa1167790:CDS 45.0%
! GATGCAAGCTTTGCTTTGGA+GGG + chr7_4:60257119-60257138 Msa1167790:CDS 45.0%
!! GCTTTGGAGGGTAAGAATGA+AGG + chr7_4:60257131-60257150 Msa1167790:CDS 45.0%
CTTGCATTTAAAGGAGGTGG+AGG - chr7_4:60257242-60257261 None:intergenic 45.0%
CTCCACCTCCTTTAAATGCA+AGG + chr7_4:60257240-60257259 Msa1167790:CDS 45.0%
CTCCTTGCATTTAAAGGAGG+TGG - chr7_4:60257245-60257264 None:intergenic 45.0%
!! ATACCTCATAGGGCACCTTT+TGG + chr7_4:60257275-60257294 Msa1167790:CDS 45.0%
! AAGATTAGGAGCCTTTGGAG+GGG + chr7_4:60257442-60257461 Msa1167790:three_prime_UTR 45.0%
!!! TTTTTATTTATTAATGTATT+TGG + chr7_4:60255722-60255741 Msa1167790:intron 5.0%
TGAGGGGGAATTATGGACGT+TGG - chr7_4:60255050-60255069 None:intergenic 50.0%
ATTGAAAGTGGTGGCTGAGG+GGG - chr7_4:60255065-60255084 None:intergenic 50.0%
GATTGAAAGTGGTGGCTGAG+GGG - chr7_4:60255066-60255085 None:intergenic 50.0%
GAGATTGAAAGTGGTGGCTG+AGG - chr7_4:60255068-60255087 None:intergenic 50.0%
CAATGGACGGAGATTTGGAG+AGG - chr7_4:60255257-60255276 None:intergenic 50.0%
GCGGTGGGATTTGGATTCAA+TGG - chr7_4:60255274-60255293 None:intergenic 50.0%
GATAATGTGGCTCAATGCGG+TGG - chr7_4:60255290-60255309 None:intergenic 50.0%
! GGTGATGATGTATGGGTGGT+GGG - chr7_4:60256257-60256276 None:intergenic 50.0%
! AGGTGATGATGTATGGGTGG+TGG - chr7_4:60256258-60256277 None:intergenic 50.0%
GCATTTAAAGGAGGTGGAGG+AGG - chr7_4:60257239-60257258 None:intergenic 50.0%
!! GAACCAAAAGGTGCCCTATG+AGG - chr7_4:60257281-60257300 None:intergenic 50.0%
! AGATTAGGAGCCTTTGGAGG+GGG + chr7_4:60257443-60257462 Msa1167790:three_prime_UTR 50.0%
TTGGACAAAACCCCCTCCAA+AGG - chr7_4:60257456-60257475 None:intergenic 50.0%
!! TTTGGAGGGGGTTTTGTCCA+AGG + chr7_4:60257455-60257474 Msa1167790:three_prime_UTR 50.0%
TGGTGGCTGAGGGGGAATTA+TGG - chr7_4:60255057-60255076 None:intergenic 55.0%
TGGCTCAATGCGGTGGGATT+TGG - chr7_4:60255283-60255302 None:intergenic 55.0%
!! ACACAGTGTTGGTGCCTCCT+TGG + chr7_4:60255429-60255448 Msa1167790:CDS 55.0%
! GCTGCATTGACAGGGGAACA+TGG + chr7_4:60256997-60257016 Msa1167790:intron 55.0%
GGGACCCAAACCGGAAAAGA+AGG - chr7_4:60257531-60257550 None:intergenic 55.0%
! GGAGGTGGAGGAGGCGTGAG+AGG - chr7_4:60257230-60257249 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 60255039 60257567 60255039 ID=Msa1167790;Name=Msa1167790
chr7_4 mRNA 60255039 60257567 60255039 ID=Msa1167790-mRNA-1;Parent=Msa1167790;Name=Msa1167790-mRNA-1;_AED=0.11;_eAED=0.11;_QI=258|1|1|1|0.75|0.6|5|257|206
chr7_4 exon 60255039 60255222 60255039 ID=Msa1167790-mRNA-1:exon:18783;Parent=Msa1167790-mRNA-1
chr7_4 exon 60255243 60255319 60255243 ID=Msa1167790-mRNA-1:exon:18784;Parent=Msa1167790-mRNA-1
chr7_4 exon 60255428 60255613 60255428 ID=Msa1167790-mRNA-1:exon:18785;Parent=Msa1167790-mRNA-1
chr7_4 exon 60255888 60256103 60255888 ID=Msa1167790-mRNA-1:exon:18786;Parent=Msa1167790-mRNA-1
chr7_4 exon 60257095 60257567 60257095 ID=Msa1167790-mRNA-1:exon:18787;Parent=Msa1167790-mRNA-1
chr7_4 five_prime_UTR 60255039 60255222 60255039 ID=Msa1167790-mRNA-1:five_prime_utr;Parent=Msa1167790-mRNA-1
chr7_4 five_prime_UTR 60255243 60255316 60255243 ID=Msa1167790-mRNA-1:five_prime_utr;Parent=Msa1167790-mRNA-1
chr7_4 CDS 60255317 60255319 60255317 ID=Msa1167790-mRNA-1:cds;Parent=Msa1167790-mRNA-1
chr7_4 CDS 60255428 60255613 60255428 ID=Msa1167790-mRNA-1:cds;Parent=Msa1167790-mRNA-1
chr7_4 CDS 60255888 60256103 60255888 ID=Msa1167790-mRNA-1:cds;Parent=Msa1167790-mRNA-1
chr7_4 CDS 60257095 60257310 60257095 ID=Msa1167790-mRNA-1:cds;Parent=Msa1167790-mRNA-1
chr7_4 three_prime_UTR 60257311 60257567 60257311 ID=Msa1167790-mRNA-1:three_prime_utr;Parent=Msa1167790-mRNA-1
Gene Sequence

>Msa1167790

ATGGACACAGTGTTGGTGCCTCCTTGGCTTGAACCATTGTTGAAAACACCGTTTTTCAACATTTGTCGGATTCACGCTGATGCAGCTAGAAATGAGTGTAACATGTATTGCTTAGATTGCAACAATGGTGATGGTGCTTTTTGCTTTTATTGTCGTTCTTCAAGACACAAAGACCATCAAGTCATTCAGATAAGAAGATCTTCATATCATGATGTAGTTCGTGTGTCAGAGATTCAGAAAGTGTTGGACATTAGTGGAGTGCAAACATATGTGATAAACAGTGCTAGAGTTTTGTTCTTGAATGTTAGGCCTCAACCAAAATCTGGGAAAGGAGTAGCTCACATTTGTGAGATTTGTGGAAGGAGCTTGTTGGATTCATTTCGGTTCTGTTCTTTGGGTTGTAAGCTTGAAAGAATAAAGAAAAATGGAGATGCAAGCTTTGCTTTGGAGGGTAAGAATGAAGGATTGACAATGGAAGGAGTGTCAAGTAGTAGATCAGAAGAAGAATTGCGTGAAGGCTCAACACAAGATATGTATCCTCTCACGCCTCCTCCACCTCCTTTAAATGCAAGGAGAAGAAAAGGTATACCTCATAGGGCACCTTTTGGTTCCTCATTCTAA
Protein sequence

>Msa1167790

MDTVLVPPWLEPLLKTPFFNICRIHADAARNECNMYCLDCNNGDGAFCFYCRSSRHKDHQVIQIRRSSYHDVVRVSEIQKVLDISGVQTYVINSARVLFLNVRPQPKSGKGVAHICEICGRSLLDSFRFCSLGCKLERIKKNGDASFALEGKNEGLTMEGVSSSRSEEELREGSTQDMYPLTPPPPPLNARRRKGIPHRAPFGSSF*