AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1168630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1168630 MtrunA17_Chr3g0136701 97.931 290 6 0 1 290 1 290 0.0 592
Msa1168630 MtrunA17_Chr1g0150471 54.717 318 114 7 1 290 1 316 9.55e-107 313
Msa1168630 MtrunA17_Chr4g0072871 51.506 332 100 11 4 280 20 345 8.29e-90 271
Msa1168630 MtrunA17_Chr7g0220721 63.043 92 34 0 3 94 23 114 1.52e-37 137
Msa1168630 MtrunA17_Chr7g0220721 71.429 70 9 2 231 289 326 395 4.94e-23 97.8
Msa1168630 MtrunA17_Chr5g0405151 50.602 83 33 1 5 79 4 86 1.00e-20 87.4
Msa1168630 MtrunA17_Chr5g0429341 43.333 90 51 0 5 94 4 93 2.37e-19 87.4
Msa1168630 MtrunA17_Chr1g0155811 43.299 97 45 1 6 92 5 101 5.14e-19 84.7
Msa1168630 MtrunA17_Chr2g0314671 44.792 96 44 1 6 92 5 100 1.09e-18 84.0
Msa1168630 MtrunA17_Chr4g0033641 43.750 96 45 1 6 92 5 100 1.14e-18 83.2
Msa1168630 MtrunA17_Chr2g0329511 42.708 96 46 1 6 92 5 100 1.62e-17 80.1
Msa1168630 MtrunA17_Chr4g0035651 39.175 97 49 1 6 92 5 101 5.31e-17 78.2
Msa1168630 MtrunA17_Chr3g0145061 38.144 97 50 1 6 92 5 101 6.06e-17 78.2
Msa1168630 MtrunA17_Chr7g0268691 39.326 89 53 1 6 94 20 107 3.25e-16 77.8
Msa1168630 MtrunA17_Chr3g0120711 39.216 102 61 1 5 106 4 104 3.54e-16 78.2
Msa1168630 MtrunA17_Chr3g0120711 44.872 78 40 1 202 279 327 401 3.25e-11 63.5
Msa1168630 MtrunA17_Chr2g0322941 45.122 82 45 0 5 86 43 124 3.09e-15 73.6
Msa1168630 MtrunA17_Chr3g0142221 38.298 94 47 1 6 88 5 98 9.45e-15 72.0
Msa1168630 MtrunA17_Chr7g0251781 44.444 72 40 0 6 77 5 76 1.38e-13 70.5
Msa1168630 MtrunA17_Chr2g0322951 38.318 107 46 2 189 279 37 139 1.25e-12 64.3
Msa1168630 MtrunA17_Chr1g0209071 39.189 74 45 0 4 77 3 76 1.46e-12 67.0
Msa1168630 MtrunA17_Chr1g0209861 61.818 55 17 1 224 274 370 424 3.03e-12 66.6
Msa1168630 MtrunA17_Chr8g0390971 70.455 44 13 0 232 275 358 401 4.48e-12 65.9
Msa1168630 MtrunA17_Chr5g0431351 66.667 45 15 0 230 274 172 216 6.93e-12 63.5
Msa1168630 MtrunA17_Chr3g0127941 49.231 65 32 1 219 283 617 680 7.03e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1168630 AT5G24930.1 52.267 375 79 13 1 289 1 361 1.78e-95 286
Msa1168630 AT2G24790.1 53.767 292 98 10 2 277 4 274 1.79e-93 278
Msa1168630 AT5G57660.1 42.775 346 131 10 2 289 18 354 5.69e-74 231
Msa1168630 AT3G02380.1 40.058 342 142 9 3 289 13 346 2.87e-72 226
Msa1168630 AT5G15850.1 41.499 347 142 8 3 289 9 354 4.46e-70 221
Msa1168630 AT5G15840.1 39.058 361 135 14 6 289 20 372 6.91e-65 208
Msa1168630 AT2G24790.2 55.367 177 58 5 2 171 4 166 3.64e-57 183
Msa1168630 AT5G15840.2 41.398 186 83 7 6 166 20 204 1.01e-36 132
Msa1168630 AT1G49130.2 28.435 313 176 10 4 280 19 319 6.30e-23 96.7
Msa1168630 AT1G49130.1 28.435 313 176 10 4 280 26 326 6.68e-23 96.7
Msa1168630 AT4G38960.1 35.570 149 74 3 5 138 4 145 3.48e-20 86.3
Msa1168630 AT4G38960.6 35.570 149 74 3 5 138 4 145 3.48e-20 86.3
Msa1168630 AT2G21320.1 49.398 83 34 1 5 79 4 86 5.96e-20 85.1
Msa1168630 AT1G78600.1 44.792 96 44 1 6 92 5 100 4.73e-19 85.5
Msa1168630 AT1G78600.2 42.017 119 56 3 6 111 5 123 5.12e-19 85.9
Msa1168630 AT2G47890.3 42.857 91 50 1 4 94 11 99 4.41e-18 82.8
Msa1168630 AT2G47890.1 42.857 91 50 1 4 94 11 99 4.93e-18 83.2
Msa1168630 AT2G47890.1 27.273 308 161 14 3 276 53 331 8.56e-14 70.9
Msa1168630 AT2G47890.2 42.857 91 50 1 4 94 11 99 6.38e-18 81.3
Msa1168630 AT1G06040.1 43.750 96 45 1 6 92 5 100 7.00e-18 81.6
Msa1168630 AT1G06040.2 43.750 96 45 1 6 92 5 100 1.05e-17 79.3
Msa1168630 AT1G75540.1 40.404 99 47 1 6 92 5 103 1.23e-17 82.0
Msa1168630 AT2G33500.1 42.697 89 51 0 6 94 12 100 3.07e-17 81.6
Msa1168630 AT2G33500.1 52.632 57 27 0 220 276 345 401 1.05e-12 68.2
Msa1168630 AT2G33500.2 42.697 89 51 0 6 94 12 100 3.08e-17 81.6
Msa1168630 AT2G33500.2 52.632 57 27 0 220 276 344 400 1.10e-12 68.2
Msa1168630 AT2G31380.1 34.177 158 87 4 6 153 5 155 1.32e-16 77.8
Msa1168630 AT1G28050.1 41.573 89 52 0 6 94 9 97 4.33e-16 78.2
Msa1168630 AT1G28050.1 56.000 50 22 0 228 277 381 430 8.98e-11 62.4
Msa1168630 AT5G48250.1 38.043 92 55 1 5 94 4 95 1.12e-14 73.9
Msa1168630 AT3G07650.3 45.205 73 40 0 5 77 4 76 3.15e-14 72.4
Msa1168630 AT3G07650.3 58.333 48 20 0 232 279 315 362 9.40e-11 62.4
Msa1168630 AT3G07650.2 45.205 73 40 0 5 77 4 76 3.15e-14 72.4
Msa1168630 AT3G07650.2 58.333 48 20 0 232 279 315 362 9.40e-11 62.4
Msa1168630 AT3G07650.4 45.205 73 40 0 5 77 4 76 3.15e-14 72.4
Msa1168630 AT3G07650.4 58.333 48 20 0 232 279 315 362 9.40e-11 62.4
Msa1168630 AT3G07650.1 45.205 73 40 0 5 77 4 76 3.15e-14 72.4
Msa1168630 AT3G07650.1 58.333 48 20 0 232 279 315 362 9.40e-11 62.4
Msa1168630 AT4G10240.1 33.714 175 81 7 6 165 5 159 4.33e-14 68.9
Msa1168630 AT4G39070.1 37.755 98 51 1 5 92 4 101 1.75e-13 68.9
Msa1168630 AT1G73870.1 55.556 63 27 1 213 275 327 388 2.42e-13 70.1
Msa1168630 AT4G15250.1 40.476 84 50 0 6 89 5 88 4.70e-13 68.9
Msa1168630 AT1G68190.2 38.824 85 50 1 4 88 41 123 6.61e-13 68.9
Msa1168630 AT1G68190.1 38.824 85 50 1 4 88 12 94 7.87e-13 68.6
Msa1168630 AT1G68190.3 38.824 85 50 1 4 88 12 94 7.87e-13 68.6
Msa1168630 AT1G25440.1 36.029 136 72 5 158 286 288 415 4.92e-12 66.2
Msa1168630 AT3G21880.1 38.202 89 53 1 6 94 5 91 1.41e-11 64.7
Msa1168630 AT3G21880.3 38.202 89 53 1 6 94 9 95 1.42e-11 64.7
Msa1168630 AT3G21880.2 38.202 89 53 1 6 94 9 95 1.42e-11 64.7
Msa1168630 AT5G14370.1 63.043 46 17 0 232 277 294 339 1.82e-11 64.3
Msa1168630 AT4G25990.1 60.870 46 18 0 232 277 341 386 2.37e-11 64.3
Msa1168630 AT1G68520.1 68.182 44 14 0 232 275 357 400 2.46e-11 64.3

Find 83 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGCAGTTGACTCCATTTC+TGG 0.143468 7_4:+61365027 Msa1168630:CDS
TCTTGCTTGCCAAACGATTT+TGG 0.181015 7_4:+61365206 Msa1168630:three_prime_UTR
ACAGAGTTTAGAAGCCATTT+TGG 0.188521 7_4:-61364091 None:intergenic
GTAATAATGTTATGGTAAAA+AGG 0.244996 7_4:+61365239 Msa1168630:three_prime_UTR
AACAGTAACCATAACCAAAA+AGG 0.245028 7_4:-61365301 None:intergenic
GAAACTAGGCCTAGGATTAA+AGG 0.280004 7_4:+61364985 Msa1168630:CDS
CCGCAGAGGAAGGCGGAGTC+AGG 0.296878 7_4:-61364153 None:intergenic
TTCCGAAGCTATTCCGTTCA+TGG 0.298068 7_4:+61364524 Msa1168630:CDS
AGGCTAGGGTTATGAGGTAT+AGG 0.358381 7_4:+61364899 Msa1168630:CDS
GACGGTGTTGTTCCCGTTCA+TGG 0.369225 7_4:+61364579 Msa1168630:CDS
GCAAACCTCCCTTTAATCCT+AGG 0.392261 7_4:-61364994 None:intergenic
CCGTTCATGGATCTCGATTA+CGG 0.395344 7_4:+61364537 Msa1168630:CDS
TTTGTTAGCGGCGTGGACTT+TGG 0.416071 7_4:-61364187 None:intergenic
GGATCGAAGTTGCCATGAAC+GGG 0.430806 7_4:-61364591 None:intergenic
TGGCGGCGGATCGTGAGGCT+AGG 0.437396 7_4:+61364884 Msa1168630:CDS
GATTCGAAGAACGGAGTAAC+GGG 0.439450 7_4:-61364363 None:intergenic
AAATTCACCCCAAAATCGTT+TGG 0.440965 7_4:-61365215 None:intergenic
AGATATCGAACTGTGGTTAC+GGG 0.444139 7_4:+61364857 Msa1168630:CDS
GAGATATCGAACTGTGGTTA+CGG 0.445942 7_4:+61364856 Msa1168630:CDS
AACGATGCCGTATCCAGAAA+TGG 0.452434 7_4:-61365040 None:intergenic
GATGAGTTGAGATCTGCTTT+CGG 0.461772 7_4:-61364483 None:intergenic
AGGGTCTGAAAGACTGCAAC+CGG 0.468857 7_4:-61365281 None:intergenic
GAGAGTGACGCGAGGGTGAC+GGG 0.479132 7_4:-61364217 None:intergenic
TCCGACACGGTGTTCCCGTC+AGG 0.479338 7_4:-61364835 None:intergenic
TTTAACAGAGTGAGAAGTGT+TGG 0.480554 7_4:-61364385 None:intergenic
GGCTAGGGTTATGAGGTATA+GGG 0.482522 7_4:+61364900 Msa1168630:CDS
TGGTTACGGGACGGTGGCGG+CGG 0.488159 7_4:+61364870 Msa1168630:CDS
AAACTAGGCCTAGGATTAAA+GGG 0.490422 7_4:+61364986 Msa1168630:CDS
AGTGTGGATGTCACGGTCAC+AGG 0.492186 7_4:-61364307 None:intergenic
ATCCATGAACGGAATAGCTT+CGG 0.492715 7_4:-61364526 None:intergenic
TTATGAGGTATAGGGAGAAG+AGG 0.496368 7_4:+61364908 Msa1168630:CDS
TTCTTGTAAGCTGAAACAAA+TGG 0.496560 7_4:-61364612 None:intergenic
GTTGACTCCATTTCTGGATA+CGG 0.500668 7_4:+61365033 Msa1168630:CDS
GGCGGCGGATCGTGAGGCTA+GGG 0.502731 7_4:+61364885 Msa1168630:CDS
TTAGGTATCGTCTTCGTCGA+TGG 0.503016 7_4:+61364798 Msa1168630:intron
ATCTGTAATAACATTCGCTT+AGG 0.508607 7_4:+61365353 Msa1168630:three_prime_UTR
GTAAAGCATATGCGGAAACT+AGG 0.510714 7_4:+61364971 Msa1168630:CDS
CTGTGGTTACGGGACGGTGG+CGG 0.513006 7_4:+61364867 Msa1168630:CDS
CGCAGAGGAAGGCGGAGTCA+GGG 0.518288 7_4:-61364152 None:intergenic
TCGTCTTCGTCGATGGAGGT+TGG 0.523017 7_4:+61364805 Msa1168630:CDS
AGGTTGGAGTTGTTCCTGAC+GGG 0.524585 7_4:+61364821 Msa1168630:CDS
ATGAAGCTGAAGCTGCTTCG+TGG 0.533741 7_4:+61364448 Msa1168630:CDS
GGATTCGAAGAACGGAGTAA+CGG 0.536432 7_4:-61364364 None:intergenic
GCTTTACACGTGACATGTGC+AGG 0.538227 7_4:-61364261 None:intergenic
AGAGAGTGACGCGAGGGTGA+CGG 0.539503 7_4:-61364218 None:intergenic
GGCGTGGACTTTGGAGTCAC+AGG 0.543388 7_4:-61364178 None:intergenic
GTCACAGGCGCCGCAGAGGA+AGG 0.545693 7_4:-61364163 None:intergenic
TCGGAATCAGAGAAAAGATA+AGG 0.547155 7_4:-61364507 None:intergenic
GACGGTGGCGGCGGATCGTG+AGG 0.549105 7_4:+61364879 Msa1168630:CDS
TGGATCGAAGTTGCCATGAA+CGG 0.558189 7_4:-61364592 None:intergenic
CGCTCGTGACGTGCGGCGAG+AGG 0.558417 7_4:-61364336 None:intergenic
GCAGTAGAGAGTAGCGGTGG+CGG 0.563031 7_4:-61364127 None:intergenic
GAAGTGTTGGATTCGAAGAA+CGG 0.565969 7_4:-61364372 None:intergenic
AAGAAGCGTTGTTATGTGCA+AGG 0.573457 7_4:-61364022 None:intergenic
GATGCAGAGAGAAGCTGCAT+CGG 0.576026 7_4:-61364283 None:intergenic
CATATGCGGAAACTAGGCCT+AGG 0.577094 7_4:+61364977 Msa1168630:CDS
ACGGGAGGCGAGTTTGTTAG+CGG 0.583367 7_4:-61364199 None:intergenic
GGTATCGTCTTCGTCGATGG+AGG 0.585063 7_4:+61364801 Msa1168630:intron
ACAGGCGCCGCAGAGGAAGG+CGG 0.586506 7_4:-61364160 None:intergenic
GATTAGCAGTGTGGATGTCA+CGG 0.589251 7_4:-61364314 None:intergenic
CGCTTCTCGTAAAGCATATG+CGG 0.592961 7_4:+61364963 Msa1168630:CDS
GAGGTTGGAGTTGTTCCTGA+CGG 0.607148 7_4:+61364820 Msa1168630:CDS
GGGAACACGCTCGTGACGTG+CGG 0.610507 7_4:-61364343 None:intergenic
ATCGTGAGGCTAGGGTTATG+AGG 0.613658 7_4:+61364893 Msa1168630:CDS
AGAAGCGTTGTTATGTGCAA+GGG 0.615745 7_4:-61364021 None:intergenic
TGGAGTCACAGGCGCCGCAG+AGG 0.616423 7_4:-61364167 None:intergenic
AGGCGAGTTTGTTAGCGGCG+TGG 0.630051 7_4:-61364194 None:intergenic
TGACTAATCTGCGACTGTGA+CGG 0.630286 7_4:-61364669 None:intergenic
GCGGCAGTAGAGAGTAGCGG+TGG 0.638763 7_4:-61364130 None:intergenic
AGGGCGGCAGTAGAGAGTAG+CGG 0.646899 7_4:-61364133 None:intergenic
CGGCGAGAGGATTAGCAGTG+TGG 0.659805 7_4:-61364323 None:intergenic
AGAGGAAGGCGGAGTCAGGG+CGG 0.663446 7_4:-61364149 None:intergenic
AGTGACGCGAGGGTGACGGG+AGG 0.667459 7_4:-61364214 None:intergenic
CCTGACTCCGCCTTCCTCTG+CGG 0.671751 7_4:+61364153 Msa1168630:CDS
ATCGAACTGTGGTTACGGGA+CGG 0.672790 7_4:+61364861 Msa1168630:CDS
TCCTGACGGGAACACCGTGT+CGG 0.682213 7_4:+61364834 Msa1168630:CDS
GAACTGTGGTTACGGGACGG+TGG 0.687113 7_4:+61364864 Msa1168630:CDS
CTTCGCAGAGAGTGACGCGA+GGG 0.694593 7_4:-61364224 None:intergenic
CTAGGCCTAGGATTAAAGGG+AGG 0.700310 7_4:+61364989 Msa1168630:CDS
ACTTCGCAGAGAGTGACGCG+AGG 0.710854 7_4:-61364225 None:intergenic
CCGTAATCGAGATCCATGAA+CGG 0.730276 7_4:-61364537 None:intergenic
ACAGTTCGATATCTCCGACA+CGG 0.744977 7_4:-61364848 None:intergenic
GTGTCGGAGATATCGAACTG+TGG 0.760977 7_4:+61364850 Msa1168630:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTGTTGTGATTAATT+AGG + chr7_4:61364780-61364799 Msa1168630:intron 15.0%
!!! TGAATTTTGTAATAATGTTA+TGG + chr7_4:61365231-61365250 Msa1168630:three_prime_UTR 15.0%
!!! TTTTTTTGTTGTGATTAATT+AGG + chr7_4:61364780-61364799 Msa1168630:intron 15.0%
!!! TGAATTTTGTAATAATGTTA+TGG + chr7_4:61365231-61365250 Msa1168630:three_prime_UTR 15.0%
!!! GAAGGAAGTTTTTTTTTTTT+TGG - chr7_4:61364072-61364091 None:intergenic 20.0%
!!! TAAGTTTGTTTTTATTGACA+TGG + chr7_4:61364721-61364740 Msa1168630:intron 20.0%
!! GTAATAATGTTATGGTAAAA+AGG + chr7_4:61365239-61365258 Msa1168630:three_prime_UTR 20.0%
!!! GAAGGAAGTTTTTTTTTTTT+TGG - chr7_4:61364072-61364091 None:intergenic 20.0%
!!! TAAGTTTGTTTTTATTGACA+TGG + chr7_4:61364721-61364740 Msa1168630:intron 20.0%
!! GTAATAATGTTATGGTAAAA+AGG + chr7_4:61365239-61365258 Msa1168630:three_prime_UTR 20.0%
! AAAAAAACTTCCTTCCAAAA+TGG + chr7_4:61364077-61364096 Msa1168630:exon 25.0%
! GTAATTGAACATAAAAACGA+CGG + chr7_4:61364561-61364580 Msa1168630:CDS 25.0%
! CAAAAAAGAAATCAACAAGT+GGG - chr7_4:61365066-61365085 None:intergenic 25.0%
! AAAAAAACTTCCTTCCAAAA+TGG + chr7_4:61364077-61364096 Msa1168630:exon 25.0%
! GTAATTGAACATAAAAACGA+CGG + chr7_4:61364561-61364580 Msa1168630:CDS 25.0%
! CAAAAAAGAAATCAACAAGT+GGG - chr7_4:61365066-61365085 None:intergenic 25.0%
TTCTTGTAAGCTGAAACAAA+TGG - chr7_4:61364615-61364634 None:intergenic 30.0%
GCAAAAAAGAAATCAACAAG+TGG - chr7_4:61365067-61365086 None:intergenic 30.0%
TATGGTAAAAAGGAAGATGA+TGG + chr7_4:61365249-61365268 Msa1168630:three_prime_UTR 30.0%
ACAGTAACCATAACCAAAAA+GGG - chr7_4:61365303-61365322 None:intergenic 30.0%
AACAGTAACCATAACCAAAA+AGG - chr7_4:61365304-61365323 None:intergenic 30.0%
ATCTGTAATAACATTCGCTT+AGG + chr7_4:61365353-61365372 Msa1168630:three_prime_UTR 30.0%
TTCTTGTAAGCTGAAACAAA+TGG - chr7_4:61364615-61364634 None:intergenic 30.0%
GCAAAAAAGAAATCAACAAG+TGG - chr7_4:61365067-61365086 None:intergenic 30.0%
TATGGTAAAAAGGAAGATGA+TGG + chr7_4:61365249-61365268 Msa1168630:three_prime_UTR 30.0%
ACAGTAACCATAACCAAAAA+GGG - chr7_4:61365303-61365322 None:intergenic 30.0%
AACAGTAACCATAACCAAAA+AGG - chr7_4:61365304-61365323 None:intergenic 30.0%
!! AGTTTAGAAGCCATTTTGGA+AGG - chr7_4:61364090-61364109 None:intergenic 35.0%
! ACAGAGTTTAGAAGCCATTT+TGG - chr7_4:61364094-61364113 None:intergenic 35.0%
! TTTAACAGAGTGAGAAGTGT+TGG - chr7_4:61364388-61364407 None:intergenic 35.0%
TCGGAATCAGAGAAAAGATA+AGG - chr7_4:61364510-61364529 None:intergenic 35.0%
AAACTAGGCCTAGGATTAAA+GGG + chr7_4:61364986-61365005 Msa1168630:CDS 35.0%
AAATTCACCCCAAAATCGTT+TGG - chr7_4:61365218-61365237 None:intergenic 35.0%
! AAGATGATGGCGAAAAATAC+CGG + chr7_4:61365262-61365281 Msa1168630:three_prime_UTR 35.0%
!!! TTTCAGACCCTTTTTGGTTA+TGG + chr7_4:61365293-61365312 Msa1168630:three_prime_UTR 35.0%
!! AGTTTAGAAGCCATTTTGGA+AGG - chr7_4:61364090-61364109 None:intergenic 35.0%
! ACAGAGTTTAGAAGCCATTT+TGG - chr7_4:61364094-61364113 None:intergenic 35.0%
! TTTAACAGAGTGAGAAGTGT+TGG - chr7_4:61364388-61364407 None:intergenic 35.0%
TCGGAATCAGAGAAAAGATA+AGG - chr7_4:61364510-61364529 None:intergenic 35.0%
AAACTAGGCCTAGGATTAAA+GGG + chr7_4:61364986-61365005 Msa1168630:CDS 35.0%
AAATTCACCCCAAAATCGTT+TGG - chr7_4:61365218-61365237 None:intergenic 35.0%
! AAGATGATGGCGAAAAATAC+CGG + chr7_4:61365262-61365281 Msa1168630:three_prime_UTR 35.0%
!!! TTTCAGACCCTTTTTGGTTA+TGG + chr7_4:61365293-61365312 Msa1168630:three_prime_UTR 35.0%
!! AGAAGCGTTGTTATGTGCAA+GGG - chr7_4:61364024-61364043 None:intergenic 40.0%
!! AAGAAGCGTTGTTATGTGCA+AGG - chr7_4:61364025-61364044 None:intergenic 40.0%
!! GAAGTGTTGGATTCGAAGAA+CGG - chr7_4:61364375-61364394 None:intergenic 40.0%
! GATGAGTTGAGATCTGCTTT+CGG - chr7_4:61364486-61364505 None:intergenic 40.0%
ATCCATGAACGGAATAGCTT+CGG - chr7_4:61364529-61364548 None:intergenic 40.0%
GAGATATCGAACTGTGGTTA+CGG + chr7_4:61364856-61364875 Msa1168630:CDS 40.0%
AGATATCGAACTGTGGTTAC+GGG + chr7_4:61364857-61364876 Msa1168630:CDS 40.0%
TTATGAGGTATAGGGAGAAG+AGG + chr7_4:61364908-61364927 Msa1168630:CDS 40.0%
GTAAAGCATATGCGGAAACT+AGG + chr7_4:61364971-61364990 Msa1168630:CDS 40.0%
GAAACTAGGCCTAGGATTAA+AGG + chr7_4:61364985-61365004 Msa1168630:CDS 40.0%
! GTTGACTCCATTTCTGGATA+CGG + chr7_4:61365033-61365052 Msa1168630:CDS 40.0%
! TCTTGCTTGCCAAACGATTT+TGG + chr7_4:61365206-61365225 Msa1168630:three_prime_UTR 40.0%
! CTTGCTTGCCAAACGATTTT+GGG + chr7_4:61365207-61365226 Msa1168630:three_prime_UTR 40.0%
! TTGCTTGCCAAACGATTTTG+GGG + chr7_4:61365208-61365227 Msa1168630:three_prime_UTR 40.0%
!! AGAAGCGTTGTTATGTGCAA+GGG - chr7_4:61364024-61364043 None:intergenic 40.0%
!! AAGAAGCGTTGTTATGTGCA+AGG - chr7_4:61364025-61364044 None:intergenic 40.0%
!! GAAGTGTTGGATTCGAAGAA+CGG - chr7_4:61364375-61364394 None:intergenic 40.0%
! GATGAGTTGAGATCTGCTTT+CGG - chr7_4:61364486-61364505 None:intergenic 40.0%
ATCCATGAACGGAATAGCTT+CGG - chr7_4:61364529-61364548 None:intergenic 40.0%
GAGATATCGAACTGTGGTTA+CGG + chr7_4:61364856-61364875 Msa1168630:CDS 40.0%
AGATATCGAACTGTGGTTAC+GGG + chr7_4:61364857-61364876 Msa1168630:CDS 40.0%
TTATGAGGTATAGGGAGAAG+AGG + chr7_4:61364908-61364927 Msa1168630:CDS 40.0%
GTAAAGCATATGCGGAAACT+AGG + chr7_4:61364971-61364990 Msa1168630:CDS 40.0%
GAAACTAGGCCTAGGATTAA+AGG + chr7_4:61364985-61365004 Msa1168630:CDS 40.0%
! GTTGACTCCATTTCTGGATA+CGG + chr7_4:61365033-61365052 Msa1168630:CDS 40.0%
! TCTTGCTTGCCAAACGATTT+TGG + chr7_4:61365206-61365225 Msa1168630:three_prime_UTR 40.0%
! CTTGCTTGCCAAACGATTTT+GGG + chr7_4:61365207-61365226 Msa1168630:three_prime_UTR 40.0%
! TTGCTTGCCAAACGATTTTG+GGG + chr7_4:61365208-61365227 Msa1168630:three_prime_UTR 40.0%
GATTAGCAGTGTGGATGTCA+CGG - chr7_4:61364317-61364336 None:intergenic 45.0%
GATTCGAAGAACGGAGTAAC+GGG - chr7_4:61364366-61364385 None:intergenic 45.0%
GGATTCGAAGAACGGAGTAA+CGG - chr7_4:61364367-61364386 None:intergenic 45.0%
TTCCGAAGCTATTCCGTTCA+TGG + chr7_4:61364524-61364543 Msa1168630:CDS 45.0%
CCGTAATCGAGATCCATGAA+CGG - chr7_4:61364540-61364559 None:intergenic 45.0%
CCGTTCATGGATCTCGATTA+CGG + chr7_4:61364537-61364556 Msa1168630:CDS 45.0%
TGGATCGAAGTTGCCATGAA+CGG - chr7_4:61364595-61364614 None:intergenic 45.0%
TGACTAATCTGCGACTGTGA+CGG - chr7_4:61364672-61364691 None:intergenic 45.0%
! TTAGGTATCGTCTTCGTCGA+TGG + chr7_4:61364798-61364817 Msa1168630:intron 45.0%
ACAGTTCGATATCTCCGACA+CGG - chr7_4:61364851-61364870 None:intergenic 45.0%
AGGCTAGGGTTATGAGGTAT+AGG + chr7_4:61364899-61364918 Msa1168630:CDS 45.0%
GGCTAGGGTTATGAGGTATA+GGG + chr7_4:61364900-61364919 Msa1168630:CDS 45.0%
CGCTTCTCGTAAAGCATATG+CGG + chr7_4:61364963-61364982 Msa1168630:CDS 45.0%
GCAAACCTCCCTTTAATCCT+AGG - chr7_4:61364997-61365016 None:intergenic 45.0%
! GATGCAGTTGACTCCATTTC+TGG + chr7_4:61365027-61365046 Msa1168630:CDS 45.0%
AACGATGCCGTATCCAGAAA+TGG - chr7_4:61365043-61365062 None:intergenic 45.0%
! GCAGTCTTTCAGACCCTTTT+TGG + chr7_4:61365287-61365306 Msa1168630:three_prime_UTR 45.0%
GATTAGCAGTGTGGATGTCA+CGG - chr7_4:61364317-61364336 None:intergenic 45.0%
GATTCGAAGAACGGAGTAAC+GGG - chr7_4:61364366-61364385 None:intergenic 45.0%
GGATTCGAAGAACGGAGTAA+CGG - chr7_4:61364367-61364386 None:intergenic 45.0%
TTCCGAAGCTATTCCGTTCA+TGG + chr7_4:61364524-61364543 Msa1168630:CDS 45.0%
CCGTAATCGAGATCCATGAA+CGG - chr7_4:61364540-61364559 None:intergenic 45.0%
CCGTTCATGGATCTCGATTA+CGG + chr7_4:61364537-61364556 Msa1168630:CDS 45.0%
TGGATCGAAGTTGCCATGAA+CGG - chr7_4:61364595-61364614 None:intergenic 45.0%
TGACTAATCTGCGACTGTGA+CGG - chr7_4:61364672-61364691 None:intergenic 45.0%
! TTAGGTATCGTCTTCGTCGA+TGG + chr7_4:61364798-61364817 Msa1168630:intron 45.0%
ACAGTTCGATATCTCCGACA+CGG - chr7_4:61364851-61364870 None:intergenic 45.0%
AGGCTAGGGTTATGAGGTAT+AGG + chr7_4:61364899-61364918 Msa1168630:CDS 45.0%
GGCTAGGGTTATGAGGTATA+GGG + chr7_4:61364900-61364919 Msa1168630:CDS 45.0%
CGCTTCTCGTAAAGCATATG+CGG + chr7_4:61364963-61364982 Msa1168630:CDS 45.0%
GCAAACCTCCCTTTAATCCT+AGG - chr7_4:61364997-61365016 None:intergenic 45.0%
! GATGCAGTTGACTCCATTTC+TGG + chr7_4:61365027-61365046 Msa1168630:CDS 45.0%
AACGATGCCGTATCCAGAAA+TGG - chr7_4:61365043-61365062 None:intergenic 45.0%
! GCAGTCTTTCAGACCCTTTT+TGG + chr7_4:61365287-61365306 Msa1168630:three_prime_UTR 45.0%
TTTGTTAGCGGCGTGGACTT+TGG - chr7_4:61364190-61364209 None:intergenic 50.0%
GCTTTACACGTGACATGTGC+AGG - chr7_4:61364264-61364283 None:intergenic 50.0%
GATGCAGAGAGAAGCTGCAT+CGG - chr7_4:61364286-61364305 None:intergenic 50.0%
ATGAAGCTGAAGCTGCTTCG+TGG + chr7_4:61364448-61364467 Msa1168630:CDS 50.0%
GGATCGAAGTTGCCATGAAC+GGG - chr7_4:61364594-61364613 None:intergenic 50.0%
GAGGTTGGAGTTGTTCCTGA+CGG + chr7_4:61364820-61364839 Msa1168630:CDS 50.0%
AGGTTGGAGTTGTTCCTGAC+GGG + chr7_4:61364821-61364840 Msa1168630:CDS 50.0%
GTGTCGGAGATATCGAACTG+TGG + chr7_4:61364850-61364869 Msa1168630:CDS 50.0%
ATCGAACTGTGGTTACGGGA+CGG + chr7_4:61364861-61364880 Msa1168630:CDS 50.0%
ATCGTGAGGCTAGGGTTATG+AGG + chr7_4:61364893-61364912 Msa1168630:CDS 50.0%
CATATGCGGAAACTAGGCCT+AGG + chr7_4:61364977-61364996 Msa1168630:CDS 50.0%
CTAGGCCTAGGATTAAAGGG+AGG + chr7_4:61364989-61365008 Msa1168630:CDS 50.0%
AGGGTCTGAAAGACTGCAAC+CGG - chr7_4:61365284-61365303 None:intergenic 50.0%
TTTGTTAGCGGCGTGGACTT+TGG - chr7_4:61364190-61364209 None:intergenic 50.0%
GCTTTACACGTGACATGTGC+AGG - chr7_4:61364264-61364283 None:intergenic 50.0%
GATGCAGAGAGAAGCTGCAT+CGG - chr7_4:61364286-61364305 None:intergenic 50.0%
ATGAAGCTGAAGCTGCTTCG+TGG + chr7_4:61364448-61364467 Msa1168630:CDS 50.0%
GGATCGAAGTTGCCATGAAC+GGG - chr7_4:61364594-61364613 None:intergenic 50.0%
GAGGTTGGAGTTGTTCCTGA+CGG + chr7_4:61364820-61364839 Msa1168630:CDS 50.0%
AGGTTGGAGTTGTTCCTGAC+GGG + chr7_4:61364821-61364840 Msa1168630:CDS 50.0%
GTGTCGGAGATATCGAACTG+TGG + chr7_4:61364850-61364869 Msa1168630:CDS 50.0%
ATCGAACTGTGGTTACGGGA+CGG + chr7_4:61364861-61364880 Msa1168630:CDS 50.0%
ATCGTGAGGCTAGGGTTATG+AGG + chr7_4:61364893-61364912 Msa1168630:CDS 50.0%
CATATGCGGAAACTAGGCCT+AGG + chr7_4:61364977-61364996 Msa1168630:CDS 50.0%
CTAGGCCTAGGATTAAAGGG+AGG + chr7_4:61364989-61365008 Msa1168630:CDS 50.0%
AGGGTCTGAAAGACTGCAAC+CGG - chr7_4:61365284-61365303 None:intergenic 50.0%
!! ACGGGAGGCGAGTTTGTTAG+CGG - chr7_4:61364202-61364221 None:intergenic 55.0%
AGTGTGGATGTCACGGTCAC+AGG - chr7_4:61364310-61364329 None:intergenic 55.0%
!! GACGGTGTTGTTCCCGTTCA+TGG + chr7_4:61364579-61364598 Msa1168630:CDS 55.0%
GGTATCGTCTTCGTCGATGG+AGG + chr7_4:61364801-61364820 Msa1168630:intron 55.0%
TCGTCTTCGTCGATGGAGGT+TGG + chr7_4:61364805-61364824 Msa1168630:CDS 55.0%
!! ACGGGAGGCGAGTTTGTTAG+CGG - chr7_4:61364202-61364221 None:intergenic 55.0%
AGTGTGGATGTCACGGTCAC+AGG - chr7_4:61364310-61364329 None:intergenic 55.0%
!! GACGGTGTTGTTCCCGTTCA+TGG + chr7_4:61364579-61364598 Msa1168630:CDS 55.0%
GGTATCGTCTTCGTCGATGG+AGG + chr7_4:61364801-61364820 Msa1168630:intron 55.0%
TCGTCTTCGTCGATGGAGGT+TGG + chr7_4:61364805-61364824 Msa1168630:CDS 55.0%
GCAGTAGAGAGTAGCGGTGG+CGG - chr7_4:61364130-61364149 None:intergenic 60.0%
AGGGCGGCAGTAGAGAGTAG+CGG - chr7_4:61364136-61364155 None:intergenic 60.0%
!! GGCGTGGACTTTGGAGTCAC+AGG - chr7_4:61364181-61364200 None:intergenic 60.0%
!! AGGCGAGTTTGTTAGCGGCG+TGG - chr7_4:61364197-61364216 None:intergenic 60.0%
AGAGAGTGACGCGAGGGTGA+CGG - chr7_4:61364221-61364240 None:intergenic 60.0%
CTTCGCAGAGAGTGACGCGA+GGG - chr7_4:61364227-61364246 None:intergenic 60.0%
ACTTCGCAGAGAGTGACGCG+AGG - chr7_4:61364228-61364247 None:intergenic 60.0%
! CGGCGAGAGGATTAGCAGTG+TGG - chr7_4:61364326-61364345 None:intergenic 60.0%
TCCTGACGGGAACACCGTGT+CGG + chr7_4:61364834-61364853 Msa1168630:CDS 60.0%
GAACTGTGGTTACGGGACGG+TGG + chr7_4:61364864-61364883 Msa1168630:CDS 60.0%
GCAGTAGAGAGTAGCGGTGG+CGG - chr7_4:61364130-61364149 None:intergenic 60.0%
AGGGCGGCAGTAGAGAGTAG+CGG - chr7_4:61364136-61364155 None:intergenic 60.0%
!! GGCGTGGACTTTGGAGTCAC+AGG - chr7_4:61364181-61364200 None:intergenic 60.0%
!! AGGCGAGTTTGTTAGCGGCG+TGG - chr7_4:61364197-61364216 None:intergenic 60.0%
AGAGAGTGACGCGAGGGTGA+CGG - chr7_4:61364221-61364240 None:intergenic 60.0%
CTTCGCAGAGAGTGACGCGA+GGG - chr7_4:61364227-61364246 None:intergenic 60.0%
ACTTCGCAGAGAGTGACGCG+AGG - chr7_4:61364228-61364247 None:intergenic 60.0%
! CGGCGAGAGGATTAGCAGTG+TGG - chr7_4:61364326-61364345 None:intergenic 60.0%
TCCTGACGGGAACACCGTGT+CGG + chr7_4:61364834-61364853 Msa1168630:CDS 60.0%
GAACTGTGGTTACGGGACGG+TGG + chr7_4:61364864-61364883 Msa1168630:CDS 60.0%
GCGGCAGTAGAGAGTAGCGG+TGG - chr7_4:61364133-61364152 None:intergenic 65.0%
AGAGGAAGGCGGAGTCAGGG+CGG - chr7_4:61364152-61364171 None:intergenic 65.0%
CGCAGAGGAAGGCGGAGTCA+GGG - chr7_4:61364155-61364174 None:intergenic 65.0%
CCTGACTCCGCCTTCCTCTG+CGG + chr7_4:61364153-61364172 Msa1168630:CDS 65.0%
GAGAGTGACGCGAGGGTGAC+GGG - chr7_4:61364220-61364239 None:intergenic 65.0%
GGGAACACGCTCGTGACGTG+CGG - chr7_4:61364346-61364365 None:intergenic 65.0%
TCCGACACGGTGTTCCCGTC+AGG - chr7_4:61364838-61364857 None:intergenic 65.0%
CTGTGGTTACGGGACGGTGG+CGG + chr7_4:61364867-61364886 Msa1168630:CDS 65.0%
GCGGCAGTAGAGAGTAGCGG+TGG - chr7_4:61364133-61364152 None:intergenic 65.0%
AGAGGAAGGCGGAGTCAGGG+CGG - chr7_4:61364152-61364171 None:intergenic 65.0%
CGCAGAGGAAGGCGGAGTCA+GGG - chr7_4:61364155-61364174 None:intergenic 65.0%
CCTGACTCCGCCTTCCTCTG+CGG + chr7_4:61364153-61364172 Msa1168630:CDS 65.0%
GAGAGTGACGCGAGGGTGAC+GGG - chr7_4:61364220-61364239 None:intergenic 65.0%
GGGAACACGCTCGTGACGTG+CGG - chr7_4:61364346-61364365 None:intergenic 65.0%
TCCGACACGGTGTTCCCGTC+AGG - chr7_4:61364838-61364857 None:intergenic 65.0%
CTGTGGTTACGGGACGGTGG+CGG + chr7_4:61364867-61364886 Msa1168630:CDS 65.0%
CCGCAGAGGAAGGCGGAGTC+AGG - chr7_4:61364156-61364175 None:intergenic 70.0%
! ACAGGCGCCGCAGAGGAAGG+CGG - chr7_4:61364163-61364182 None:intergenic 70.0%
! GTCACAGGCGCCGCAGAGGA+AGG - chr7_4:61364166-61364185 None:intergenic 70.0%
! TGGAGTCACAGGCGCCGCAG+AGG - chr7_4:61364170-61364189 None:intergenic 70.0%
!! AGTGACGCGAGGGTGACGGG+AGG - chr7_4:61364217-61364236 None:intergenic 70.0%
! TGGTTACGGGACGGTGGCGG+CGG + chr7_4:61364870-61364889 Msa1168630:CDS 70.0%
TGGCGGCGGATCGTGAGGCT+AGG + chr7_4:61364884-61364903 Msa1168630:CDS 70.0%
GGCGGCGGATCGTGAGGCTA+GGG + chr7_4:61364885-61364904 Msa1168630:CDS 70.0%
CCGCAGAGGAAGGCGGAGTC+AGG - chr7_4:61364156-61364175 None:intergenic 70.0%
! ACAGGCGCCGCAGAGGAAGG+CGG - chr7_4:61364163-61364182 None:intergenic 70.0%
! GTCACAGGCGCCGCAGAGGA+AGG - chr7_4:61364166-61364185 None:intergenic 70.0%
! TGGAGTCACAGGCGCCGCAG+AGG - chr7_4:61364170-61364189 None:intergenic 70.0%
!! AGTGACGCGAGGGTGACGGG+AGG - chr7_4:61364217-61364236 None:intergenic 70.0%
! TGGTTACGGGACGGTGGCGG+CGG + chr7_4:61364870-61364889 Msa1168630:CDS 70.0%
TGGCGGCGGATCGTGAGGCT+AGG + chr7_4:61364884-61364903 Msa1168630:CDS 70.0%
GGCGGCGGATCGTGAGGCTA+GGG + chr7_4:61364885-61364904 Msa1168630:CDS 70.0%
CGCTCGTGACGTGCGGCGAG+AGG - chr7_4:61364339-61364358 None:intergenic 75.0%
! GACGGTGGCGGCGGATCGTG+AGG + chr7_4:61364879-61364898 Msa1168630:CDS 75.0%
CGCTCGTGACGTGCGGCGAG+AGG - chr7_4:61364339-61364358 None:intergenic 75.0%
! GACGGTGGCGGCGGATCGTG+AGG + chr7_4:61364879-61364898 Msa1168630:CDS 75.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 61364013 61365375 61364013 ID=Msa1168630;Name=Msa1168630
chr7_4 mRNA 61364013 61365375 61364013 ID=Msa1168630-mRNA-1;Parent=Msa1168630;Name=Msa1168630-mRNA-1;_AED=0.00;_eAED=0.00;_QI=83|1|1|1|1|1|2|301|290
chr7_4 exon 61364013 61364695 61364013 ID=Msa1168630-mRNA-1:exon:19374;Parent=Msa1168630-mRNA-1
chr7_4 exon 61364802 61365375 61364802 ID=Msa1168630-mRNA-1:exon:19375;Parent=Msa1168630-mRNA-1
chr7_4 five_prime_UTR 61364013 61364095 61364013 ID=Msa1168630-mRNA-1:five_prime_utr;Parent=Msa1168630-mRNA-1
chr7_4 CDS 61364096 61364695 61364096 ID=Msa1168630-mRNA-1:cds;Parent=Msa1168630-mRNA-1
chr7_4 CDS 61364802 61365074 61364802 ID=Msa1168630-mRNA-1:cds;Parent=Msa1168630-mRNA-1
chr7_4 three_prime_UTR 61365075 61365375 61365075 ID=Msa1168630-mRNA-1:three_prime_utr;Parent=Msa1168630-mRNA-1
Gene Sequence

>Msa1168630

ATGGCTTCTAAACTCTGTGACTCATGCAAATCCGCCACCGCTACTCTCTACTGCCGCCCTGACTCCGCCTTCCTCTGCGGCGCCTGTGACTCCAAAGTCCACGCCGCTAACAAACTCGCCTCCCGTCACCCTCGCGTCACTCTCTGCGAAGTCTGCGAACAAGCACCTGCACATGTCACGTGTAAAGCCGATGCAGCTTCTCTCTGCATCACCTGTGACCGTGACATCCACACTGCTAATCCTCTCGCCGCACGTCACGAGCGTGTTCCCGTTACTCCGTTCTTCGAATCCAACACTTCTCACTCTGTTAAATCGCTTCAAAACAATAACAACAACTACGATGCTGTTAAAGATGAAGCTGAAGCTGCTTCGTGGTTGATCTCTGATCCGAAAGCAGATCTCAACTCATCTCCTTATCTTTTCTCTGATTCCGAAGCTATTCCGTTCATGGATCTCGATTACGGTGTAATTGAACATAAAAACGACGGTGTTGTTCCCGTTCATGGCAACTTCGATCCATTTGTTTCAGCTTACAAGAACAACAACGTGCACTTGCATACAGAGTTAGAAACACCGTCACAGTCGCAGATTAGTCAGAGTGTATCGTCTTCGTCGATGGAGGTTGGAGTTGTTCCTGACGGGAACACCGTGTCGGAGATATCGAACTGTGGTTACGGGACGGTGGCGGCGGATCGTGAGGCTAGGGTTATGAGGTATAGGGAGAAGAGGAAGAATAGAAGATTCGAGAAGACGATTCGTTACGCTTCTCGTAAAGCATATGCGGAAACTAGGCCTAGGATTAAAGGGAGGTTTGCAAAACGCACTGATGCAGTTGACTCCATTTCTGGATACGGCATCGTTCCCACTTGTTGA
Protein sequence

>Msa1168630

MASKLCDSCKSATATLYCRPDSAFLCGACDSKVHAANKLASRHPRVTLCEVCEQAPAHVTCKADAASLCITCDRDIHTANPLAARHERVPVTPFFESNTSHSVKSLQNNNNNYDAVKDEAEAASWLISDPKADLNSSPYLFSDSEAIPFMDLDYGVIEHKNDGVVPVHGNFDPFVSAYKNNNVHLHTELETPSQSQISQSVSSSSMEVGVVPDGNTVSEISNCGYGTVAADREARVMRYREKRKNRRFEKTIRYASRKAYAETRPRIKGRFAKRTDAVDSISGYGIVPTC*