AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1168800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1168800 MtrunA17_Chr7g0264171 93.820 178 11 0 1 178 87 264 1.26e-121 356
Msa1168800 MtrunA17_Chr7g0264161 88.750 160 18 0 1 160 48 207 6.53e-104 300
Msa1168800 MtrunA17_Chr2g0326761 61.364 176 63 2 1 176 67 237 2.57e-66 214
Msa1168800 MtrunA17_Chr1g0199681 66.216 148 50 0 1 148 149 296 2.45e-64 209
Msa1168800 MtrunA17_Chr1g0181211 67.320 153 42 2 1 148 83 232 4.44e-63 206
Msa1168800 MtrunA17_Chr4g0028971 58.889 180 70 3 1 177 66 244 1.83e-60 199
Msa1168800 MtrunA17_Chr3g0115271 54.667 150 61 2 1 148 76 220 5.75e-49 166
Msa1168800 MtrunA17_Chr8g0389081 52.000 150 66 2 2 149 95 240 2.50e-46 162
Msa1168800 MtrunA17_Chr1g0156321 51.007 149 70 1 2 150 106 251 5.14e-46 160
Msa1168800 MtrunA17_Chr4g0070181 48.667 150 71 2 2 149 85 230 2.61e-44 156
Msa1168800 MtrunA17_Chr2g0301251 49.333 150 70 2 2 149 85 230 5.93e-44 155
Msa1168800 MtrunA17_Chr2g0285461 48.026 152 75 2 2 152 86 234 4.20e-42 149
Msa1168800 MtrunA17_Chr2g0282961 48.684 152 71 1 2 146 108 259 8.38e-42 147
Msa1168800 MtrunA17_Chr5g0424931 50.000 150 61 2 1 145 78 218 9.93e-42 146
Msa1168800 MtrunA17_Chr2g0276781 47.682 151 74 2 2 151 85 231 4.34e-41 146
Msa1168800 MtrunA17_Chr4g0029671 48.428 159 74 1 1 151 114 272 8.24e-41 145
Msa1168800 MtrunA17_Chr3g0109391 48.366 153 73 3 2 152 89 237 1.44e-40 145
Msa1168800 MtrunA17_Chr5g0433301 47.712 153 74 3 2 152 87 235 1.96e-40 145
Msa1168800 MtrunA17_Chr2g0289221 48.649 148 70 3 1 145 80 224 4.78e-40 139
Msa1168800 MtrunA17_Chr8g0376771 46.980 149 73 2 2 148 85 229 6.15e-40 143
Msa1168800 MtrunA17_Chr4g0046371 43.182 176 88 2 2 165 94 269 6.88e-40 143
Msa1168800 MtrunA17_Chr5g0417281 46.753 154 69 3 1 148 70 216 9.25e-40 141
Msa1168800 MtrunA17_Chr2g0326281 45.349 172 73 3 1 151 116 287 9.45e-40 143
Msa1168800 MtrunA17_Chr4g0009751 47.333 150 74 2 1 149 80 225 6.71e-39 139
Msa1168800 MtrunA17_Chr8g0385791 45.238 168 85 4 2 166 121 284 9.39e-39 140
Msa1168800 MtrunA17_Chr8g0346711 46.711 152 76 2 1 151 90 237 1.29e-37 136
Msa1168800 MtrunA17_Chr7g0239061 46.528 144 74 2 1 144 75 215 4.52e-37 135
Msa1168800 MtrunA17_Chr5g0417211 45.513 156 69 3 1 147 71 219 3.20e-35 127
Msa1168800 MtrunA17_Chr5g0436761 43.312 157 72 3 1 147 71 220 1.54e-32 120
Msa1168800 MtrunA17_Chr1g0178011 41.892 148 78 2 1 145 71 213 2.90e-32 120
Msa1168800 MtrunA17_Chr2g0277461 41.781 146 82 3 1 145 74 217 1.81e-31 117
Msa1168800 MtrunA17_Chr5g0425661 38.028 142 70 3 1 133 93 225 5.14e-26 99.4
Msa1168800 MtrunA17_Chr8g0356211 42.424 99 57 0 53 151 2 100 3.69e-25 99.0
Msa1168800 MtrunA17_Chr2g0277531 37.838 148 85 5 1 145 81 224 2.84e-22 91.3
Msa1168800 MtrunA17_Chr5g0424801 33.333 147 73 5 6 145 76 204 1.64e-17 79.0
Msa1168800 MtrunA17_Chr5g0424741 31.977 172 88 6 1 145 71 240 6.75e-16 74.7
Msa1168800 MtrunA17_Chr5g0425261 32.000 150 77 5 6 148 76 207 1.95e-15 72.8
Msa1168800 MtrunA17_Chr8g0345771 30.709 127 83 2 40 162 167 292 9.40e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1168800 AT2G28350.2 63.636 154 50 1 1 148 65 218 8.73e-63 205
Msa1168800 AT2G28350.1 63.636 154 50 1 1 148 65 218 1.16e-62 205
Msa1168800 AT4G30080.1 59.524 168 59 3 1 159 74 241 8.64e-60 197
Msa1168800 AT1G77850.2 50.676 148 71 1 1 146 73 220 5.72e-46 159
Msa1168800 AT1G77850.1 50.676 148 71 1 1 146 73 220 6.39e-46 159
Msa1168800 AT1G19220.1 50.667 150 68 2 2 149 85 230 1.20e-44 157
Msa1168800 AT5G20730.3 49.007 151 71 2 2 150 86 232 9.97e-44 155
Msa1168800 AT5G20730.2 49.007 151 71 2 2 150 86 232 1.19e-43 154
Msa1168800 AT5G20730.1 49.007 151 71 2 2 150 86 232 1.19e-43 154
Msa1168800 AT1G19850.1 49.007 151 75 1 2 152 117 265 5.46e-43 152
Msa1168800 AT1G19850.2 49.007 151 75 1 2 152 106 254 5.73e-43 152
Msa1168800 AT1G30330.1 49.669 151 74 2 2 152 86 234 2.54e-41 148
Msa1168800 AT1G30330.3 49.669 151 74 2 2 152 88 236 2.54e-41 148
Msa1168800 AT1G30330.2 49.669 151 74 2 2 152 88 236 2.54e-41 148
Msa1168800 AT5G37020.2 47.712 153 74 3 2 152 85 233 3.60e-41 147
Msa1168800 AT5G60450.2 47.170 159 76 2 1 151 101 259 3.72e-41 147
Msa1168800 AT5G60450.1 47.170 159 76 2 1 151 125 283 4.03e-41 147
Msa1168800 AT5G37020.1 47.712 153 74 3 2 152 85 233 5.28e-41 147
Msa1168800 AT1G59750.4 50.000 150 72 3 2 151 84 230 1.41e-40 145
Msa1168800 AT1G59750.2 50.000 150 72 3 2 151 84 230 1.49e-40 145
Msa1168800 AT1G59750.1 50.000 150 72 3 2 151 84 230 1.72e-40 145
Msa1168800 AT1G59750.3 50.000 150 72 3 2 151 84 230 1.72e-40 145
Msa1168800 AT2G33860.1 45.455 154 77 1 2 148 109 262 7.87e-40 142
Msa1168800 AT3G61830.2 44.805 154 76 2 2 152 37 184 1.73e-38 138
Msa1168800 AT3G61830.1 44.805 154 76 2 2 152 88 235 3.08e-38 138
Msa1168800 AT5G62000.2 43.452 168 88 3 2 166 123 286 9.18e-38 137
Msa1168800 AT5G62000.5 43.452 168 88 3 2 166 123 286 9.18e-38 137
Msa1168800 AT5G62000.3 43.452 168 88 3 2 166 123 286 9.18e-38 137
Msa1168800 AT5G62000.1 43.452 168 88 3 2 166 123 286 9.18e-38 137
Msa1168800 AT5G62000.4 43.452 168 88 3 2 166 123 286 9.89e-38 137
Msa1168800 AT5G37020.3 54.630 108 49 0 45 152 31 138 1.72e-37 136
Msa1168800 AT4G23980.2 46.575 146 74 2 2 146 75 217 2.87e-37 135
Msa1168800 AT4G23980.1 46.575 146 74 2 2 146 75 217 3.05e-37 135
Msa1168800 AT2G33860.2 56.731 104 45 0 45 148 2 105 4.14e-37 133
Msa1168800 AT2G46530.1 41.420 169 89 3 2 166 84 246 6.55e-37 134
Msa1168800 AT2G46530.3 41.420 169 89 3 2 166 105 267 6.86e-37 134
Msa1168800 AT1G35520.1 44.295 149 77 2 2 150 89 231 4.37e-34 126
Msa1168800 AT1G35540.1 46.099 141 70 2 6 146 93 227 8.02e-34 125
Msa1168800 AT1G34310.1 43.448 145 76 1 6 150 93 231 3.57e-33 124
Msa1168800 AT1G35240.1 43.448 145 76 1 6 150 93 231 6.99e-33 123
Msa1168800 AT1G34410.1 43.709 151 75 2 2 150 89 231 1.16e-32 122
Msa1168800 AT2G46530.2 45.161 124 67 1 44 166 36 159 1.74e-32 121
Msa1168800 AT1G34390.1 44.138 145 73 2 2 146 89 225 6.67e-32 120
Msa1168800 AT1G34170.2 40.000 155 77 4 2 150 87 231 2.40e-28 110
Msa1168800 AT1G34170.1 40.000 155 77 4 2 150 87 231 2.87e-28 110
Msa1168800 AT1G34170.3 40.000 155 77 4 2 150 87 231 3.62e-28 110
Msa1168800 AT1G43950.1 39.568 139 75 4 6 144 93 222 3.34e-23 92.0
Msa1168800 AT2G46870.1 36.082 97 61 1 37 133 27 122 3.78e-13 66.6
Msa1168800 AT1G01030.2 34.545 110 66 2 40 144 51 159 3.13e-11 61.2
Msa1168800 AT3G25730.1 30.952 126 70 2 45 168 183 293 4.80e-11 60.5
Msa1168800 AT1G01030.1 34.545 110 66 2 40 144 51 159 4.83e-11 60.8
Msa1168800 AT4G01500.1 31.200 125 73 4 18 133 1 121 6.48e-11 60.1

Find 46 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTGTCAGGGTGGAAATT+TGG 0.238329 7_4:+61629884 Msa1168800:CDS
CATCAACGTGTCTGAAATTA+AGG 0.241218 7_4:+61629514 Msa1168800:CDS
GCATTTCAGGCATGTTTATA+GGG 0.274751 7_4:+61629703 Msa1168800:CDS
GGCATTTCAGGCATGTTTAT+AGG 0.293252 7_4:+61629702 Msa1168800:CDS
ATGGTGAAAAGTGGCATTTC+AGG 0.316984 7_4:+61629690 Msa1168800:CDS
TGGAAATTTGGTAGTGGAAT+TGG 0.321491 7_4:+61629896 Msa1168800:CDS
TTGGAATTCGGAGGGCTAAA+AGG 0.363443 7_4:+61629840 Msa1168800:CDS
TTTGATAATGATGGTGTTGC+TGG 0.376636 7_4:+61629491 Msa1168800:CDS
AGTGACAAGAAACTTGCTTC+TGG 0.385156 7_4:+61629773 Msa1168800:CDS
CTAACTTGGTTAATATTCAT+AGG 0.385591 7_4:-61629467 None:intergenic
AAGAGGCATTTGTTGACAAC+TGG 0.392584 7_4:+61629734 Msa1168800:CDS
AGTTTCTTGTCACTGACATA+TGG 0.394738 7_4:-61629764 None:intergenic
GTTGGAGTCAATCCTTTGTC+AGG 0.400283 7_4:+61629872 Msa1168800:CDS
GTGAACTTAATGTTGGAATT+CGG 0.402297 7_4:+61629828 Msa1168800:CDS
GTGACAAGAAACTTGCTTCT+GGG 0.423889 7_4:+61629774 Msa1168800:CDS
CATCATTATCAAAACTAACT+TGG 0.449861 7_4:-61629481 None:intergenic
GTTGTCAACAAATGCCTCTT+CGG 0.456311 7_4:-61629731 None:intergenic
ATTTGTCCTGCACCACCTTA+CGG 0.476461 7_4:+61629920 Msa1168800:CDS
GAAAACAGTGAACTTAATGT+TGG 0.494744 7_4:+61629821 Msa1168800:CDS
TCAGGGTGGAAATTTGGTAG+TGG 0.505938 7_4:+61629890 Msa1168800:CDS
TTGGGATAGATGTCCTGAAA+AGG 0.529804 7_4:-61629659 None:intergenic
AATCCTCCGTAAGGTGGTGC+AGG 0.533772 7_4:-61629926 None:intergenic
TGACAAGAAACTTGCTTCTG+GGG 0.545499 7_4:+61629775 Msa1168800:CDS
CCAAATTTCCACCCTGACAA+AGG 0.546733 7_4:-61629884 None:intergenic
GCTAAAAGGCGAAATAATGT+TGG 0.555377 7_4:+61629854 Msa1168800:CDS
CATTTCAGGCATGTTTATAG+GGG 0.556177 7_4:+61629704 Msa1168800:CDS
AACTATGGAAATCCTCCGTA+AGG 0.559080 7_4:-61629935 None:intergenic
AGCGTTTCGGCACAATATCT+AGG 0.559188 7_4:-61629605 None:intergenic
GGCATTTGTTGACAACTGGA+TGG 0.569244 7_4:+61629738 Msa1168800:CDS
AACTTAATGTTGGAATTCGG+AGG 0.572730 7_4:+61629831 Msa1168800:CDS
AGGATGTACATGGTGAAAAG+TGG 0.579569 7_4:+61629681 Msa1168800:CDS
TCTGATGCAAACACTGGTGG+TGG 0.592766 7_4:+61629572 Msa1168800:CDS
ATCTATCCCAAGGATGTACA+TGG 0.592936 7_4:+61629671 Msa1168800:CDS
GGATAGATGTCCTGAAAAGG+AGG 0.594077 7_4:-61629656 None:intergenic
TTGGAGTCAATCCTTTGTCA+GGG 0.598239 7_4:+61629873 Msa1168800:CDS
ATGAAGTGTATGCAAAACTC+AGG 0.607388 7_4:+61629438 Msa1168800:CDS
TTTATAGGGGCACGCCGAAG+AGG 0.616360 7_4:+61629717 Msa1168800:CDS
GTTTGCATCAGATTGTGTCA+AGG 0.616878 7_4:-61629561 None:intergenic
ACACAATCTGATGCAAACAC+TGG 0.627018 7_4:+61629566 Msa1168800:CDS
ACTTAATGTTGGAATTCGGA+GGG 0.629032 7_4:+61629832 Msa1168800:CDS
TATGGAAATCCTCCGTAAGG+TGG 0.634599 7_4:-61629932 None:intergenic
GAGTCAATCCTTTGTCAGGG+TGG 0.657457 7_4:+61629876 Msa1168800:CDS
TGTCCTGCACCACCTTACGG+AGG 0.685773 7_4:+61629923 Msa1168800:CDS
GATAGATGTCCTGAAAAGGA+GGG 0.691897 7_4:-61629655 None:intergenic
CAATCTGATGCAAACACTGG+TGG 0.725224 7_4:+61629569 Msa1168800:CDS
TTAGCTGAATAGTCCAAACG+AGG 0.756003 7_4:-61629632 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTAACTTGGTTAATATTCAT+AGG - chr7_4:61629470-61629489 None:intergenic 25.0%
! CATCATTATCAAAACTAACT+TGG - chr7_4:61629484-61629503 None:intergenic 25.0%
!!! CAAGTTAGTTTTGATAATGA+TGG + chr7_4:61629482-61629501 Msa1168800:CDS 25.0%
GAAAACAGTGAACTTAATGT+TGG + chr7_4:61629821-61629840 Msa1168800:CDS 30.0%
GTGAACTTAATGTTGGAATT+CGG + chr7_4:61629828-61629847 Msa1168800:CDS 30.0%
! GAGTTTTGCATACACTTCAT+CGG - chr7_4:61629438-61629457 None:intergenic 35.0%
ATGAAGTGTATGCAAAACTC+AGG + chr7_4:61629438-61629457 Msa1168800:CDS 35.0%
!! TTTGATAATGATGGTGTTGC+TGG + chr7_4:61629491-61629510 Msa1168800:CDS 35.0%
! CATCAACGTGTCTGAAATTA+AGG + chr7_4:61629514-61629533 Msa1168800:CDS 35.0%
! TTTTCACCATGTACATCCTT+GGG - chr7_4:61629680-61629699 None:intergenic 35.0%
GCATTTCAGGCATGTTTATA+GGG + chr7_4:61629703-61629722 Msa1168800:CDS 35.0%
CATTTCAGGCATGTTTATAG+GGG + chr7_4:61629704-61629723 Msa1168800:CDS 35.0%
AGTTTCTTGTCACTGACATA+TGG - chr7_4:61629767-61629786 None:intergenic 35.0%
AACTTAATGTTGGAATTCGG+AGG + chr7_4:61629831-61629850 Msa1168800:CDS 35.0%
ACTTAATGTTGGAATTCGGA+GGG + chr7_4:61629832-61629851 Msa1168800:CDS 35.0%
GCTAAAAGGCGAAATAATGT+TGG + chr7_4:61629854-61629873 Msa1168800:CDS 35.0%
!! TGGAAATTTGGTAGTGGAAT+TGG + chr7_4:61629896-61629915 Msa1168800:CDS 35.0%
GTTTGCATCAGATTGTGTCA+AGG - chr7_4:61629564-61629583 None:intergenic 40.0%
ACACAATCTGATGCAAACAC+TGG + chr7_4:61629566-61629585 Msa1168800:CDS 40.0%
!! CAAACGAGGAAAAAGCGTTT+CGG - chr7_4:61629621-61629640 None:intergenic 40.0%
TTAGCTGAATAGTCCAAACG+AGG - chr7_4:61629635-61629654 None:intergenic 40.0%
GATAGATGTCCTGAAAAGGA+GGG - chr7_4:61629658-61629677 None:intergenic 40.0%
TTGGGATAGATGTCCTGAAA+AGG - chr7_4:61629662-61629681 None:intergenic 40.0%
!! TTTTCAGGACATCTATCCCA+AGG + chr7_4:61629661-61629680 Msa1168800:CDS 40.0%
ATCTATCCCAAGGATGTACA+TGG + chr7_4:61629671-61629690 Msa1168800:CDS 40.0%
! CTTTTCACCATGTACATCCT+TGG - chr7_4:61629681-61629700 None:intergenic 40.0%
AGGATGTACATGGTGAAAAG+TGG + chr7_4:61629681-61629700 Msa1168800:CDS 40.0%
ATGGTGAAAAGTGGCATTTC+AGG + chr7_4:61629690-61629709 Msa1168800:CDS 40.0%
GGCATTTCAGGCATGTTTAT+AGG + chr7_4:61629702-61629721 Msa1168800:CDS 40.0%
GTTGTCAACAAATGCCTCTT+CGG - chr7_4:61629734-61629753 None:intergenic 40.0%
!! AAGAGGCATTTGTTGACAAC+TGG + chr7_4:61629734-61629753 Msa1168800:CDS 40.0%
! AGTGACAAGAAACTTGCTTC+TGG + chr7_4:61629773-61629792 Msa1168800:CDS 40.0%
! GTGACAAGAAACTTGCTTCT+GGG + chr7_4:61629774-61629793 Msa1168800:CDS 40.0%
! TGACAAGAAACTTGCTTCTG+GGG + chr7_4:61629775-61629794 Msa1168800:CDS 40.0%
TTGGAGTCAATCCTTTGTCA+GGG + chr7_4:61629873-61629892 Msa1168800:CDS 40.0%
CAATCTGATGCAAACACTGG+TGG + chr7_4:61629569-61629588 Msa1168800:CDS 45.0%
AGCGTTTCGGCACAATATCT+AGG - chr7_4:61629608-61629627 None:intergenic 45.0%
!!! CGAAACGCTTTTTCCTCGTT+TGG + chr7_4:61629619-61629638 Msa1168800:CDS 45.0%
! TTCAGCTAACCCTCCTTTTC+AGG + chr7_4:61629646-61629665 Msa1168800:CDS 45.0%
GGATAGATGTCCTGAAAAGG+AGG - chr7_4:61629659-61629678 None:intergenic 45.0%
! GGCATTTGTTGACAACTGGA+TGG + chr7_4:61629738-61629757 Msa1168800:CDS 45.0%
TTGGAATTCGGAGGGCTAAA+AGG + chr7_4:61629840-61629859 Msa1168800:CDS 45.0%
GTTGGAGTCAATCCTTTGTC+AGG + chr7_4:61629872-61629891 Msa1168800:CDS 45.0%
CCAAATTTCCACCCTGACAA+AGG - chr7_4:61629887-61629906 None:intergenic 45.0%
CCTTTGTCAGGGTGGAAATT+TGG + chr7_4:61629884-61629903 Msa1168800:CDS 45.0%
TCAGGGTGGAAATTTGGTAG+TGG + chr7_4:61629890-61629909 Msa1168800:CDS 45.0%
ATTTGTCCTGCACCACCTTA+CGG + chr7_4:61629920-61629939 Msa1168800:CDS 45.0%
TATGGAAATCCTCCGTAAGG+TGG - chr7_4:61629935-61629954 None:intergenic 45.0%
TCTGATGCAAACACTGGTGG+TGG + chr7_4:61629572-61629591 Msa1168800:CDS 50.0%
GAGTCAATCCTTTGTCAGGG+TGG + chr7_4:61629876-61629895 Msa1168800:CDS 50.0%
! TTTATAGGGGCACGCCGAAG+AGG + chr7_4:61629717-61629736 Msa1168800:CDS 55.0%
AATCCTCCGTAAGGTGGTGC+AGG - chr7_4:61629929-61629948 None:intergenic 55.0%
TGTCCTGCACCACCTTACGG+AGG + chr7_4:61629923-61629942 Msa1168800:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 61629419 61629955 61629419 ID=Msa1168800;Name=Msa1168800
chr7_4 mRNA 61629419 61629955 61629419 ID=Msa1168800-mRNA-1;Parent=Msa1168800;Name=Msa1168800-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|178
chr7_4 exon 61629419 61629955 61629419 ID=Msa1168800-mRNA-1:exon:19475;Parent=Msa1168800-mRNA-1
chr7_4 CDS 61629419 61629955 61629419 ID=Msa1168800-mRNA-1:cds;Parent=Msa1168800-mRNA-1
Gene Sequence

>Msa1168800

ATGGCTAATCATGAGACCGATGAAGTGTATGCAAAACTCAGGCTTGTTCCTATGAATATTAACCAAGTTAGTTTTGATAATGATGGTGTTGCTGGCATCAACGTGTCTGAAATTAAGGATAAACATCAATCTTTTGCGAAAACCTTGACACAATCTGATGCAAACACTGGTGGTGGCTTTTCTTGTCCTAGATATTGTGCCGAAACGCTTTTTCCTCGTTTGGACTATTCAGCTAACCCTCCTTTTCAGGACATCTATCCCAAGGATGTACATGGTGAAAAGTGGCATTTCAGGCATGTTTATAGGGGCACGCCGAAGAGGCATTTGTTGACAACTGGATGGAGTCCATATGTCAGTGACAAGAAACTTGCTTCTGGGGATTCAATTGTGTTTTTGAGATCTGAAAACAGTGAACTTAATGTTGGAATTCGGAGGGCTAAAAGGCGAAATAATGTTGGAGTCAATCCTTTGTCAGGGTGGAAATTTGGTAGTGGAATTGGAATTTGTCCTGCACCACCTTACGGAGGATTTCCATAG
Protein sequence

>Msa1168800

MANHETDEVYAKLRLVPMNINQVSFDNDGVAGINVSEIKDKHQSFAKTLTQSDANTGGGFSCPRYCAETLFPRLDYSANPPFQDIYPKDVHGEKWHFRHVYRGTPKRHLLTTGWSPYVSDKKLASGDSIVFLRSENSELNVGIRRAKRRNNVGVNPLSGWKFGSGIGICPAPPYGGFP*