AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1168870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1168870 MtrunA17_Chr7g0264171 94.944 178 9 0 1 178 87 264 4.67e-123 359
Msa1168870 MtrunA17_Chr7g0264161 88.750 160 18 0 1 160 48 207 5.61e-104 300
Msa1168870 MtrunA17_Chr2g0326761 61.932 176 62 2 1 176 67 237 1.58e-67 218
Msa1168870 MtrunA17_Chr1g0199681 66.892 148 49 0 1 148 149 296 1.97e-65 212
Msa1168870 MtrunA17_Chr1g0181211 68.627 153 40 2 1 148 83 232 1.87e-64 209
Msa1168870 MtrunA17_Chr4g0028971 59.444 180 69 3 1 177 66 244 1.17e-61 202
Msa1168870 MtrunA17_Chr3g0115271 56.000 150 59 2 1 148 76 220 1.70e-50 171
Msa1168870 MtrunA17_Chr8g0389081 51.333 150 67 2 2 149 95 240 8.38e-46 160
Msa1168870 MtrunA17_Chr1g0156321 49.669 151 73 1 2 152 106 253 1.21e-45 159
Msa1168870 MtrunA17_Chr4g0070181 48.000 150 72 2 2 149 85 230 7.63e-44 154
Msa1168870 MtrunA17_Chr2g0301251 48.667 150 71 2 2 149 85 230 2.27e-43 153
Msa1168870 MtrunA17_Chr5g0424931 50.667 150 60 2 1 145 78 218 1.27e-42 149
Msa1168870 MtrunA17_Chr2g0285461 47.368 152 76 2 2 152 86 234 1.12e-41 148
Msa1168870 MtrunA17_Chr2g0282961 48.026 152 72 1 2 146 108 259 4.03e-41 145
Msa1168870 MtrunA17_Chr2g0289221 48.344 151 72 3 1 148 80 227 4.68e-41 141
Msa1168870 MtrunA17_Chr4g0029671 47.799 159 75 1 1 151 114 272 3.50e-40 144
Msa1168870 MtrunA17_Chr2g0276781 47.020 151 75 2 2 151 85 231 3.98e-40 143
Msa1168870 MtrunA17_Chr3g0109391 47.712 153 74 3 2 152 89 237 4.09e-40 144
Msa1168870 MtrunA17_Chr5g0433301 47.059 153 75 3 2 152 87 235 4.97e-40 144
Msa1168870 MtrunA17_Chr5g0417281 46.753 154 69 3 1 148 70 216 8.61e-40 141
Msa1168870 MtrunA17_Chr8g0376771 46.309 149 74 2 2 148 85 229 1.93e-39 142
Msa1168870 MtrunA17_Chr4g0046371 42.614 176 89 2 2 165 94 269 3.01e-39 141
Msa1168870 MtrunA17_Chr2g0326281 44.767 172 74 3 1 151 116 287 4.27e-39 141
Msa1168870 MtrunA17_Chr4g0009751 46.667 150 75 2 1 149 80 225 1.87e-38 138
Msa1168870 MtrunA17_Chr8g0385791 44.643 168 86 4 2 166 121 284 3.29e-38 138
Msa1168870 MtrunA17_Chr8g0346711 46.053 152 77 2 1 151 90 237 3.49e-37 135
Msa1168870 MtrunA17_Chr7g0239061 45.833 144 75 2 1 144 75 215 1.17e-36 134
Msa1168870 MtrunA17_Chr5g0417211 46.154 156 68 3 1 147 71 219 4.60e-36 129
Msa1168870 MtrunA17_Chr5g0436761 43.949 157 71 3 1 147 71 220 2.00e-33 123
Msa1168870 MtrunA17_Chr1g0178011 42.568 148 77 2 1 145 71 213 4.45e-33 122
Msa1168870 MtrunA17_Chr2g0277461 42.466 146 81 3 1 145 74 217 2.51e-32 120
Msa1168870 MtrunA17_Chr5g0425661 38.028 142 70 3 1 133 93 225 6.56e-26 99.4
Msa1168870 MtrunA17_Chr8g0356211 41.414 99 58 0 53 151 2 100 9.13e-25 97.8
Msa1168870 MtrunA17_Chr2g0277531 37.838 148 85 5 1 145 81 224 2.72e-22 91.3
Msa1168870 MtrunA17_Chr5g0424801 33.333 147 73 5 6 145 76 204 1.22e-17 79.0
Msa1168870 MtrunA17_Chr5g0425261 32.000 150 77 5 6 148 76 207 1.41e-15 73.2
Msa1168870 MtrunA17_Chr5g0424741 31.395 172 89 6 1 145 71 240 2.16e-15 73.2
Msa1168870 MtrunA17_Chr8g0345771 30.709 127 83 2 40 162 167 292 9.95e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1168870 AT2G28350.2 64.286 154 49 1 1 148 65 218 6.01e-64 208
Msa1168870 AT2G28350.1 64.286 154 49 1 1 148 65 218 8.52e-64 208
Msa1168870 AT4G30080.1 60.119 168 58 3 1 159 74 241 7.82e-61 200
Msa1168870 AT1G77850.1 51.351 148 70 1 1 146 73 220 2.64e-47 162
Msa1168870 AT1G77850.2 51.351 148 70 1 1 146 73 220 3.02e-47 162
Msa1168870 AT1G19220.1 50.000 150 69 2 2 149 85 230 3.32e-44 156
Msa1168870 AT5G20730.3 48.344 151 72 2 2 150 86 232 2.89e-43 153
Msa1168870 AT5G20730.2 48.344 151 72 2 2 150 86 232 3.16e-43 153
Msa1168870 AT5G20730.1 48.344 151 72 2 2 150 86 232 3.22e-43 153
Msa1168870 AT1G19850.2 48.344 151 76 1 2 152 106 254 1.27e-42 151
Msa1168870 AT1G19850.1 48.344 151 76 1 2 152 117 265 1.32e-42 151
Msa1168870 AT1G30330.1 49.007 151 75 2 2 152 86 234 7.90e-41 146
Msa1168870 AT1G30330.3 49.007 151 75 2 2 152 88 236 8.29e-41 146
Msa1168870 AT1G30330.2 49.007 151 75 2 2 152 88 236 8.29e-41 146
Msa1168870 AT5G37020.2 47.059 153 75 3 2 152 85 233 1.05e-40 145
Msa1168870 AT5G37020.1 47.059 153 75 3 2 152 85 233 1.32e-40 145
Msa1168870 AT5G60450.2 46.541 159 77 2 1 151 101 259 1.46e-40 145
Msa1168870 AT5G60450.1 46.541 159 77 2 1 151 125 283 1.62e-40 145
Msa1168870 AT1G59750.4 49.333 150 73 3 2 151 84 230 1.22e-39 142
Msa1168870 AT1G59750.2 49.333 150 73 3 2 151 84 230 1.22e-39 142
Msa1168870 AT1G59750.1 49.333 150 73 3 2 151 84 230 1.28e-39 142
Msa1168870 AT1G59750.3 49.333 150 73 3 2 151 84 230 1.28e-39 142
Msa1168870 AT2G33860.1 44.805 154 78 1 2 148 109 262 2.92e-39 141
Msa1168870 AT3G61830.1 44.156 154 77 2 2 152 88 235 6.41e-38 137
Msa1168870 AT3G61830.2 45.333 150 79 1 2 151 37 183 2.78e-37 135
Msa1168870 AT5G62000.2 42.857 168 89 3 2 166 123 286 2.99e-37 136
Msa1168870 AT5G62000.5 42.857 168 89 3 2 166 123 286 2.99e-37 136
Msa1168870 AT5G62000.3 42.857 168 89 3 2 166 123 286 2.99e-37 136
Msa1168870 AT5G62000.1 42.857 168 89 3 2 166 123 286 2.99e-37 136
Msa1168870 AT5G62000.4 45.033 151 77 2 2 150 123 269 3.29e-37 136
Msa1168870 AT5G37020.3 53.704 108 50 0 45 152 31 138 5.38e-37 135
Msa1168870 AT4G23980.2 45.890 146 75 2 2 146 75 217 7.25e-37 134
Msa1168870 AT4G23980.1 45.890 146 75 2 2 146 75 217 7.77e-37 134
Msa1168870 AT2G46530.1 40.828 169 90 3 2 166 84 246 1.55e-36 133
Msa1168870 AT2G46530.3 40.828 169 90 3 2 166 105 267 1.60e-36 133
Msa1168870 AT2G33860.2 55.769 104 46 0 45 148 2 105 1.75e-36 131
Msa1168870 AT1G35520.1 45.517 145 73 2 6 150 93 231 5.28e-34 126
Msa1168870 AT1G35540.1 46.099 141 70 2 6 146 93 227 1.32e-33 125
Msa1168870 AT1G34310.1 43.448 145 76 1 6 150 93 231 4.40e-33 124
Msa1168870 AT1G35240.1 43.448 145 76 1 6 150 93 231 7.87e-33 123
Msa1168870 AT1G34410.1 44.898 147 71 2 6 150 93 231 1.26e-32 122
Msa1168870 AT2G46530.2 44.355 124 68 1 44 166 36 159 4.11e-32 120
Msa1168870 AT1G34390.1 44.681 141 70 2 6 146 93 225 1.62e-31 119
Msa1168870 AT1G34170.2 40.000 155 77 4 2 150 87 231 3.06e-28 109
Msa1168870 AT1G34170.1 40.000 155 77 4 2 150 87 231 3.48e-28 109
Msa1168870 AT1G34170.3 40.000 155 77 4 2 150 87 231 4.55e-28 109
Msa1168870 AT1G43950.1 39.568 139 75 4 6 144 93 222 3.41e-23 92.0
Msa1168870 AT2G46870.1 36.082 97 61 1 37 133 27 122 3.04e-13 66.6
Msa1168870 AT3G25730.1 30.952 126 70 2 45 168 183 293 2.42e-11 61.6
Msa1168870 AT4G01500.1 31.200 125 73 4 18 133 1 121 2.79e-11 61.2
Msa1168870 AT1G01030.2 34.545 110 66 2 40 144 51 159 3.69e-11 60.8
Msa1168870 AT1G01030.1 34.545 110 66 2 40 144 51 159 5.07e-11 60.5

Find 48 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTGTCAGGGTGGAAATT+TGG 0.238329 7_4:+61754708 Msa1168870:CDS
CATCAACGTGTCTGAAATTA+AGG 0.241218 7_4:+61754338 Msa1168870:CDS
GCATTTCAGGCATGTTTATA+GGG 0.274751 7_4:+61754527 Msa1168870:CDS
GGCATTTCAGGCATGTTTAT+AGG 0.293252 7_4:+61754526 Msa1168870:CDS
ATGGTGAAAAGTGGCATTTC+AGG 0.316984 7_4:+61754514 Msa1168870:CDS
TGGAAATTTGGTAGTGGAAT+TGG 0.321491 7_4:+61754720 Msa1168870:CDS
TTCAGCTAACCCTCCTCTTC+AGG 0.361334 7_4:+61754470 Msa1168870:CDS
TTGGAATTCGGAGGGCTAAA+AGG 0.363443 7_4:+61754664 Msa1168870:CDS
TTTGATAATGATGGTGTTGC+TGG 0.376636 7_4:+61754315 Msa1168870:CDS
AGTGACAAGAAACTTGCTTC+TGG 0.385156 7_4:+61754597 Msa1168870:CDS
CTAACTTGGTTAATATTCAT+AGG 0.385591 7_4:-61754291 None:intergenic
AAGAGGCATTTGTTGACAAC+TGG 0.392584 7_4:+61754558 Msa1168870:CDS
AGTTTCTTGTCACTGACATA+TGG 0.394738 7_4:-61754588 None:intergenic
GTTGGAGTCAATCCTTTGTC+AGG 0.400283 7_4:+61754696 Msa1168870:CDS
GTGAACTTAATGTTGGAATT+CGG 0.402297 7_4:+61754652 Msa1168870:CDS
GTGACAAGAAACTTGCTTCT+GGG 0.423889 7_4:+61754598 Msa1168870:CDS
CATCATTATCAAAACTAACT+TGG 0.449861 7_4:-61754305 None:intergenic
GTTGTCAACAAATGCCTCTT+CGG 0.456311 7_4:-61754555 None:intergenic
ATTTGTCCTGCACCACCTTA+CGG 0.476461 7_4:+61754744 Msa1168870:CDS
GAAAACAGTGAACTTAATGT+TGG 0.494744 7_4:+61754645 Msa1168870:CDS
TCAGGGTGGAAATTTGGTAG+TGG 0.505938 7_4:+61754714 Msa1168870:CDS
AATCCTCCGTAAGGTGGTGC+AGG 0.533772 7_4:-61754750 None:intergenic
AGCGTTTCTGCACAATATCT+AGG 0.540389 7_4:-61754429 None:intergenic
TGACAAGAAACTTGCTTCTG+GGG 0.545499 7_4:+61754599 Msa1168870:CDS
CCAAATTTCCACCCTGACAA+AGG 0.546733 7_4:-61754708 None:intergenic
GCTAAAAGGCGAAATAATGT+TGG 0.555377 7_4:+61754678 Msa1168870:CDS
CATTTCAGGCATGTTTATAG+GGG 0.556177 7_4:+61754528 Msa1168870:CDS
AACTATGGAAATCCTCCGTA+AGG 0.559080 7_4:-61754759 None:intergenic
GGCATTTGTTGACAACTGGA+TGG 0.569244 7_4:+61754562 Msa1168870:CDS
AACTTAATGTTGGAATTCGG+AGG 0.572730 7_4:+61754655 Msa1168870:CDS
AGGATGTACATGGTGAAAAG+TGG 0.579569 7_4:+61754505 Msa1168870:CDS
GGATAGATGTCCTGAAGAGG+AGG 0.591495 7_4:-61754480 None:intergenic
ATCTATCCCAAGGATGTACA+TGG 0.592936 7_4:+61754495 Msa1168870:CDS
TTGGAGTCAATCCTTTGTCA+GGG 0.598239 7_4:+61754697 Msa1168870:CDS
GTTTGCATCAGATTGTGTCA+AGG 0.603398 7_4:-61754385 None:intergenic
ATGAAGTGTATGCAAAACTC+AGG 0.607388 7_4:+61754262 Msa1168870:CDS
TTTATAGGGGCACGCCGAAG+AGG 0.616360 7_4:+61754541 Msa1168870:CDS
TCTTCAGGACATCTATCCCA+AGG 0.626591 7_4:+61754485 Msa1168870:CDS
ACTTAATGTTGGAATTCGGA+GGG 0.629032 7_4:+61754656 Msa1168870:CDS
TTGGGATAGATGTCCTGAAG+AGG 0.634276 7_4:-61754483 None:intergenic
TATGGAAATCCTCCGTAAGG+TGG 0.634599 7_4:-61754756 None:intergenic
TCTGATGCAAACAATGGTGG+TGG 0.655667 7_4:+61754396 Msa1168870:CDS
GAGTCAATCCTTTGTCAGGG+TGG 0.657457 7_4:+61754700 Msa1168870:CDS
TGTCCTGCACCACCTTACGG+AGG 0.685773 7_4:+61754747 Msa1168870:CDS
GATAGATGTCCTGAAGAGGA+GGG 0.691087 7_4:-61754479 None:intergenic
ACACAATCTGATGCAAACAA+TGG 0.696099 7_4:+61754390 Msa1168870:CDS
CAATCTGATGCAAACAATGG+TGG 0.722659 7_4:+61754393 Msa1168870:CDS
TTAGCTGAATAGTCCAAACG+AGG 0.756003 7_4:-61754456 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTAACTTGGTTAATATTCAT+AGG - chr7_4:61754294-61754313 None:intergenic 25.0%
! CATCATTATCAAAACTAACT+TGG - chr7_4:61754308-61754327 None:intergenic 25.0%
!!! CAAGTTAGTTTTGATAATGA+TGG + chr7_4:61754306-61754325 Msa1168870:CDS 25.0%
GAAAACAGTGAACTTAATGT+TGG + chr7_4:61754645-61754664 Msa1168870:CDS 30.0%
GTGAACTTAATGTTGGAATT+CGG + chr7_4:61754652-61754671 Msa1168870:CDS 30.0%
! GAGTTTTGCATACACTTCAT+CGG - chr7_4:61754262-61754281 None:intergenic 35.0%
ATGAAGTGTATGCAAAACTC+AGG + chr7_4:61754262-61754281 Msa1168870:CDS 35.0%
!! TTTGATAATGATGGTGTTGC+TGG + chr7_4:61754315-61754334 Msa1168870:CDS 35.0%
! CATCAACGTGTCTGAAATTA+AGG + chr7_4:61754338-61754357 Msa1168870:CDS 35.0%
ACACAATCTGATGCAAACAA+TGG + chr7_4:61754390-61754409 Msa1168870:CDS 35.0%
! TTTTCACCATGTACATCCTT+GGG - chr7_4:61754504-61754523 None:intergenic 35.0%
GCATTTCAGGCATGTTTATA+GGG + chr7_4:61754527-61754546 Msa1168870:CDS 35.0%
CATTTCAGGCATGTTTATAG+GGG + chr7_4:61754528-61754547 Msa1168870:CDS 35.0%
AGTTTCTTGTCACTGACATA+TGG - chr7_4:61754591-61754610 None:intergenic 35.0%
AACTTAATGTTGGAATTCGG+AGG + chr7_4:61754655-61754674 Msa1168870:CDS 35.0%
ACTTAATGTTGGAATTCGGA+GGG + chr7_4:61754656-61754675 Msa1168870:CDS 35.0%
GCTAAAAGGCGAAATAATGT+TGG + chr7_4:61754678-61754697 Msa1168870:CDS 35.0%
!! TGGAAATTTGGTAGTGGAAT+TGG + chr7_4:61754720-61754739 Msa1168870:CDS 35.0%
GTTTGCATCAGATTGTGTCA+AGG - chr7_4:61754388-61754407 None:intergenic 40.0%
CAATCTGATGCAAACAATGG+TGG + chr7_4:61754393-61754412 Msa1168870:CDS 40.0%
AGCGTTTCTGCACAATATCT+AGG - chr7_4:61754432-61754451 None:intergenic 40.0%
!!! AGAAACGCTTTTTCCTCGTT+TGG + chr7_4:61754443-61754462 Msa1168870:CDS 40.0%
TTAGCTGAATAGTCCAAACG+AGG - chr7_4:61754459-61754478 None:intergenic 40.0%
ATCTATCCCAAGGATGTACA+TGG + chr7_4:61754495-61754514 Msa1168870:CDS 40.0%
! CTTTTCACCATGTACATCCT+TGG - chr7_4:61754505-61754524 None:intergenic 40.0%
AGGATGTACATGGTGAAAAG+TGG + chr7_4:61754505-61754524 Msa1168870:CDS 40.0%
ATGGTGAAAAGTGGCATTTC+AGG + chr7_4:61754514-61754533 Msa1168870:CDS 40.0%
GGCATTTCAGGCATGTTTAT+AGG + chr7_4:61754526-61754545 Msa1168870:CDS 40.0%
GTTGTCAACAAATGCCTCTT+CGG - chr7_4:61754558-61754577 None:intergenic 40.0%
!! AAGAGGCATTTGTTGACAAC+TGG + chr7_4:61754558-61754577 Msa1168870:CDS 40.0%
! AGTGACAAGAAACTTGCTTC+TGG + chr7_4:61754597-61754616 Msa1168870:CDS 40.0%
! GTGACAAGAAACTTGCTTCT+GGG + chr7_4:61754598-61754617 Msa1168870:CDS 40.0%
! TGACAAGAAACTTGCTTCTG+GGG + chr7_4:61754599-61754618 Msa1168870:CDS 40.0%
TTGGAGTCAATCCTTTGTCA+GGG + chr7_4:61754697-61754716 Msa1168870:CDS 40.0%
TCTGATGCAAACAATGGTGG+TGG + chr7_4:61754396-61754415 Msa1168870:CDS 45.0%
GATAGATGTCCTGAAGAGGA+GGG - chr7_4:61754482-61754501 None:intergenic 45.0%
TTGGGATAGATGTCCTGAAG+AGG - chr7_4:61754486-61754505 None:intergenic 45.0%
TCTTCAGGACATCTATCCCA+AGG + chr7_4:61754485-61754504 Msa1168870:CDS 45.0%
! GGCATTTGTTGACAACTGGA+TGG + chr7_4:61754562-61754581 Msa1168870:CDS 45.0%
TTGGAATTCGGAGGGCTAAA+AGG + chr7_4:61754664-61754683 Msa1168870:CDS 45.0%
GTTGGAGTCAATCCTTTGTC+AGG + chr7_4:61754696-61754715 Msa1168870:CDS 45.0%
CCAAATTTCCACCCTGACAA+AGG - chr7_4:61754711-61754730 None:intergenic 45.0%
CCTTTGTCAGGGTGGAAATT+TGG + chr7_4:61754708-61754727 Msa1168870:CDS 45.0%
TCAGGGTGGAAATTTGGTAG+TGG + chr7_4:61754714-61754733 Msa1168870:CDS 45.0%
ATTTGTCCTGCACCACCTTA+CGG + chr7_4:61754744-61754763 Msa1168870:CDS 45.0%
TATGGAAATCCTCCGTAAGG+TGG - chr7_4:61754759-61754778 None:intergenic 45.0%
TTCAGCTAACCCTCCTCTTC+AGG + chr7_4:61754470-61754489 Msa1168870:CDS 50.0%
GGATAGATGTCCTGAAGAGG+AGG - chr7_4:61754483-61754502 None:intergenic 50.0%
GAGTCAATCCTTTGTCAGGG+TGG + chr7_4:61754700-61754719 Msa1168870:CDS 50.0%
! TTTATAGGGGCACGCCGAAG+AGG + chr7_4:61754541-61754560 Msa1168870:CDS 55.0%
AATCCTCCGTAAGGTGGTGC+AGG - chr7_4:61754753-61754772 None:intergenic 55.0%
TGTCCTGCACCACCTTACGG+AGG + chr7_4:61754747-61754766 Msa1168870:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 61754243 61754779 61754243 ID=Msa1168870;Name=Msa1168870
chr7_4 mRNA 61754243 61754779 61754243 ID=Msa1168870-mRNA-1;Parent=Msa1168870;Name=Msa1168870-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|178
chr7_4 exon 61754243 61754779 61754243 ID=Msa1168870-mRNA-1:exon:19497;Parent=Msa1168870-mRNA-1
chr7_4 CDS 61754243 61754779 61754243 ID=Msa1168870-mRNA-1:cds;Parent=Msa1168870-mRNA-1
Gene Sequence

>Msa1168870

ATGGCTAATCATGAGACCGATGAAGTGTATGCAAAACTCAGGCTTGTTCCTATGAATATTAACCAAGTTAGTTTTGATAATGATGGTGTTGCTGGCATCAACGTGTCTGAAATTAAGGATAAACATCAATCTTTTGCGAAAACCTTGACACAATCTGATGCAAACAATGGTGGTGGCTTTTCTTGTCCTAGATATTGTGCAGAAACGCTTTTTCCTCGTTTGGACTATTCAGCTAACCCTCCTCTTCAGGACATCTATCCCAAGGATGTACATGGTGAAAAGTGGCATTTCAGGCATGTTTATAGGGGCACGCCGAAGAGGCATTTGTTGACAACTGGATGGAGTCCATATGTCAGTGACAAGAAACTTGCTTCTGGGGATTCAATTGTGTTTTTGAGATCTGAAAACAGTGAACTTAATGTTGGAATTCGGAGGGCTAAAAGGCGAAATAATGTTGGAGTCAATCCTTTGTCAGGGTGGAAATTTGGTAGTGGAATTGGAATTTGTCCTGCACCACCTTACGGAGGATTTCCATAG
Protein sequence

>Msa1168870

MANHETDEVYAKLRLVPMNINQVSFDNDGVAGINVSEIKDKHQSFAKTLTQSDANNGGGFSCPRYCAETLFPRLDYSANPPLQDIYPKDVHGEKWHFRHVYRGTPKRHLLTTGWSPYVSDKKLASGDSIVFLRSENSELNVGIRRAKRRNNVGVNPLSGWKFGSGIGICPAPPYGGFP*