Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1171550 | AES61431.2 | 91.007 | 278 | 10 | 1 | 1 | 263 | 1 | 278 | 0.0 | 515 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1171550 | sp|Q9SZL8|FRS5_ARATH | 25.328 | 229 | 154 | 5 | 35 | 263 | 72 | 283 | 4.29e-13 | 72.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa1171550 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1171550 | MtrunA17_Chr1g0172971 | 88.889 | 117 | 13 | 0 | 1 | 117 | 1 | 117 | 1.09e-68 | 221 |
| Msa1171550 | MtrunA17_Chr6g0475591 | 93.151 | 73 | 5 | 0 | 189 | 261 | 1 | 73 | 1.37e-43 | 142 |
| Msa1171550 | MtrunA17_Chr7g0258861 | 27.500 | 240 | 144 | 6 | 31 | 263 | 2 | 218 | 5.38e-23 | 95.9 |
| Msa1171550 | MtrunA17_Chr2g0324011 | 24.686 | 239 | 149 | 7 | 32 | 263 | 3 | 217 | 2.35e-17 | 81.6 |
| Msa1171550 | MtrunA17_Chr1g0202341 | 28.085 | 235 | 145 | 6 | 35 | 267 | 50 | 262 | 9.37e-17 | 79.7 |
| Msa1171550 | MtrunA17_Chr3g0082121 | 23.874 | 222 | 142 | 4 | 42 | 263 | 49 | 243 | 2.08e-14 | 72.8 |
| Msa1171550 | MtrunA17_Chr5g0448761 | 23.810 | 231 | 160 | 4 | 35 | 265 | 59 | 273 | 1.48e-12 | 67.4 |
| Msa1171550 | MtrunA17_Chr8g0363841 | 23.556 | 225 | 149 | 6 | 41 | 263 | 22 | 225 | 5.56e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1171550 | AT4G38180.1 | 25.328 | 229 | 154 | 5 | 35 | 263 | 72 | 283 | 4.36e-14 | 72.4 |
Find 41 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGATGTTGGCCGGTTGTT+AGG | 0.327171 | 8_1:-1232845 | None:intergenic |
| TTTAAGTCTGAAAACCAAAA+AGG | 0.346249 | 8_1:-1233163 | None:intergenic |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 8_1:+1233111 | Msa1171550:CDS |
| CACAGTCCTAATAGAAAATC+CGG | 0.413322 | 8_1:-1232720 | None:intergenic |
| GAAGTTATGGGTCAAGGAGT+TGG | 0.428685 | 8_1:+1233124 | Msa1171550:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 8_1:+1233049 | Msa1171550:CDS |
| ATGAGTATAAGATATATAGA+TGG | 0.430188 | 8_1:+1232966 | Msa1171550:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 8_1:+1232914 | Msa1171550:CDS |
| GCTCTAATAAATTCTAAAGC+TGG | 0.462891 | 8_1:+1233079 | Msa1171550:CDS |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 8_1:+1233112 | Msa1171550:CDS |
| TCTAATGACAATTCATCAAA+AGG | 0.474120 | 8_1:+1233192 | Msa1171550:CDS |
| CCCCTAGCAATAACGAATCA+TGG | 0.476678 | 8_1:+1232633 | Msa1171550:CDS |
| TCCATGATTCGTTATTGCTA+GGG | 0.479627 | 8_1:-1232634 | None:intergenic |
| TTACTTCTCGAAATGATCTC+AGG | 0.488412 | 8_1:-1233036 | None:intergenic |
| GTTTGCTTAATCCTAACAAC+CGG | 0.493754 | 8_1:+1232834 | Msa1171550:CDS |
| CTCAGGTAACACAAATGCTC+TGG | 0.496027 | 8_1:-1233019 | None:intergenic |
| AGCTGGAATATCTTTGAAGC+TGG | 0.496270 | 8_1:+1232931 | Msa1171550:CDS |
| TGTCTTCCGCAGCATCAACT+TGG | 0.498648 | 8_1:-1233297 | None:intergenic |
| TTACAACCAACCCTTTCCTC+AGG | 0.505082 | 8_1:-1232908 | None:intergenic |
| TTCCATGATTCGTTATTGCT+AGG | 0.509536 | 8_1:-1232635 | None:intergenic |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | 8_1:+1232808 | Msa1171550:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.514358 | 8_1:-1233007 | None:intergenic |
| TGAGTATAAGATATATAGAT+GGG | 0.523615 | 8_1:+1232967 | Msa1171550:CDS |
| ACAAAATAAGAAATCAGATA+AGG | 0.530342 | 8_1:+1232763 | Msa1171550:CDS |
| GACAATTCATCAAAAGGAGA+TGG | 0.553152 | 8_1:+1233198 | Msa1171550:CDS |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | 8_1:+1232809 | Msa1171550:CDS |
| GGTAATTATGCATATAACAC+CGG | 0.561940 | 8_1:+1232701 | Msa1171550:CDS |
| CATCAAAAGGAGATGGTGGT+TGG | 0.563657 | 8_1:+1233205 | Msa1171550:CDS |
| TAAGATATATAGATGGGATG+TGG | 0.566406 | 8_1:+1232973 | Msa1171550:CDS |
| CCATGATTCGTTATTGCTAG+GGG | 0.572877 | 8_1:-1232633 | None:intergenic |
| GTTATGGGTCAAGGAGTTGG+AGG | 0.578191 | 8_1:+1233127 | Msa1171550:CDS |
| TCTTATGAAGTTATGGGTCA+AGG | 0.580120 | 8_1:+1233118 | Msa1171550:CDS |
| ATATCAACTTCATTTGAGAG+TGG | 0.601371 | 8_1:+1232554 | Msa1171550:CDS |
| AATTCATCAAAAGGAGATGG+TGG | 0.606871 | 8_1:+1233201 | Msa1171550:CDS |
| GTGGACTGTCTGATGTTGGC+CGG | 0.608598 | 8_1:-1232853 | None:intergenic |
| GCCAACATCAGACAGTCCAC+TGG | 0.611268 | 8_1:+1232856 | Msa1171550:CDS |
| TTCACAAACTCAATAACCAG+TGG | 0.633415 | 8_1:-1232872 | None:intergenic |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.637387 | 8_1:+1233132 | Msa1171550:CDS |
| ACCAGTGGACTGTCTGATGT+TGG | 0.641808 | 8_1:-1232857 | None:intergenic |
| TGTTATGTTTGTAACAAACA+AGG | 0.647419 | 8_1:+1232797 | Msa1171550:CDS |
| TGACATCCAAGTTGATGCTG+CGG | 0.654084 | 8_1:+1233291 | Msa1171550:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGAGATTGAAGATTTTTA+TGG | + | chr8_1:1232680-1232699 | Msa1171550:CDS | 20.0% |
| !! | ACAAAATAAGAAATCAGATA+AGG | + | chr8_1:1232763-1232782 | Msa1171550:CDS | 20.0% |
| !! | ATGAGTATAAGATATATAGA+TGG | + | chr8_1:1232966-1232985 | Msa1171550:CDS | 20.0% |
| !! | TGAGTATAAGATATATAGAT+GGG | + | chr8_1:1232967-1232986 | Msa1171550:CDS | 20.0% |
| !!! | AAAGAGATTGAAGATTTTTA+TGG | + | chr8_1:1232680-1232699 | Msa1171550:CDS | 20.0% |
| !! | ACAAAATAAGAAATCAGATA+AGG | + | chr8_1:1232763-1232782 | Msa1171550:CDS | 20.0% |
| !! | ATGAGTATAAGATATATAGA+TGG | + | chr8_1:1232966-1232985 | Msa1171550:CDS | 20.0% |
| !! | TGAGTATAAGATATATAGAT+GGG | + | chr8_1:1232967-1232986 | Msa1171550:CDS | 20.0% |
| ! | TGTTATGTTTGTAACAAACA+AGG | + | chr8_1:1232797-1232816 | Msa1171550:CDS | 25.0% |
| ! | TTTAAGTCTGAAAACCAAAA+AGG | - | chr8_1:1233166-1233185 | None:intergenic | 25.0% |
| ! | TCTAATGACAATTCATCAAA+AGG | + | chr8_1:1233192-1233211 | Msa1171550:CDS | 25.0% |
| ! | TGTTATGTTTGTAACAAACA+AGG | + | chr8_1:1232797-1232816 | Msa1171550:CDS | 25.0% |
| ! | TTTAAGTCTGAAAACCAAAA+AGG | - | chr8_1:1233166-1233185 | None:intergenic | 25.0% |
| ! | TCTAATGACAATTCATCAAA+AGG | + | chr8_1:1233192-1233211 | Msa1171550:CDS | 25.0% |
| ATATCAACTTCATTTGAGAG+TGG | + | chr8_1:1232554-1232573 | Msa1171550:CDS | 30.0% | |
| GGTAATTATGCATATAACAC+CGG | + | chr8_1:1232701-1232720 | Msa1171550:CDS | 30.0% | |
| ! | ATAACACCGGATTTTCTATT+AGG | + | chr8_1:1232714-1232733 | Msa1171550:CDS | 30.0% |
| TAACAAACAAGGTTTCAAGA+AGG | + | chr8_1:1232808-1232827 | Msa1171550:CDS | 30.0% | |
| AACAAACAAGGTTTCAAGAA+GGG | + | chr8_1:1232809-1232828 | Msa1171550:CDS | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | + | chr8_1:1232973-1232992 | Msa1171550:CDS | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | - | chr8_1:1233010-1233029 | None:intergenic | 30.0% |
| !! | GCTCTAATAAATTCTAAAGC+TGG | + | chr8_1:1233079-1233098 | Msa1171550:CDS | 30.0% |
| AGAACGTCTTATGAAGTTAT+GGG | + | chr8_1:1233112-1233131 | Msa1171550:CDS | 30.0% | |
| TGGATGTCATAGTAAAAAGA+TGG | - | chr8_1:1233280-1233299 | None:intergenic | 30.0% | |
| ATATCAACTTCATTTGAGAG+TGG | + | chr8_1:1232554-1232573 | Msa1171550:CDS | 30.0% | |
| GGTAATTATGCATATAACAC+CGG | + | chr8_1:1232701-1232720 | Msa1171550:CDS | 30.0% | |
| ! | ATAACACCGGATTTTCTATT+AGG | + | chr8_1:1232714-1232733 | Msa1171550:CDS | 30.0% |
| TAACAAACAAGGTTTCAAGA+AGG | + | chr8_1:1232808-1232827 | Msa1171550:CDS | 30.0% | |
| AACAAACAAGGTTTCAAGAA+GGG | + | chr8_1:1232809-1232828 | Msa1171550:CDS | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | + | chr8_1:1232973-1232992 | Msa1171550:CDS | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | - | chr8_1:1233010-1233029 | None:intergenic | 30.0% |
| !! | GCTCTAATAAATTCTAAAGC+TGG | + | chr8_1:1233079-1233098 | Msa1171550:CDS | 30.0% |
| AGAACGTCTTATGAAGTTAT+GGG | + | chr8_1:1233112-1233131 | Msa1171550:CDS | 30.0% | |
| TGGATGTCATAGTAAAAAGA+TGG | - | chr8_1:1233280-1233299 | None:intergenic | 30.0% | |
| TCCATGATTCGTTATTGCTA+GGG | - | chr8_1:1232637-1232656 | None:intergenic | 35.0% | |
| TTCCATGATTCGTTATTGCT+AGG | - | chr8_1:1232638-1232657 | None:intergenic | 35.0% | |
| CACAGTCCTAATAGAAAATC+CGG | - | chr8_1:1232723-1232742 | None:intergenic | 35.0% | |
| GTTTGCTTAATCCTAACAAC+CGG | + | chr8_1:1232834-1232853 | Msa1171550:CDS | 35.0% | |
| TTCACAAACTCAATAACCAG+TGG | - | chr8_1:1232875-1232894 | None:intergenic | 35.0% | |
| GAAAGTGAAAGAAAAACCTG+AGG | + | chr8_1:1232892-1232911 | Msa1171550:CDS | 35.0% | |
| TGAAAGAAAAACCTGAGGAA+AGG | + | chr8_1:1232897-1232916 | Msa1171550:CDS | 35.0% | |
| GAAAGAAAAACCTGAGGAAA+GGG | + | chr8_1:1232898-1232917 | Msa1171550:CDS | 35.0% | |
| TTACTTCTCGAAATGATCTC+AGG | - | chr8_1:1233039-1233058 | None:intergenic | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | + | chr8_1:1233049-1233068 | Msa1171550:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | + | chr8_1:1233111-1233130 | Msa1171550:CDS | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | + | chr8_1:1233118-1233137 | Msa1171550:CDS | 35.0% | |
| GACAATTCATCAAAAGGAGA+TGG | + | chr8_1:1233198-1233217 | Msa1171550:CDS | 35.0% | |
| AATTCATCAAAAGGAGATGG+TGG | + | chr8_1:1233201-1233220 | Msa1171550:CDS | 35.0% | |
| TCCATGATTCGTTATTGCTA+GGG | - | chr8_1:1232637-1232656 | None:intergenic | 35.0% | |
| TTCCATGATTCGTTATTGCT+AGG | - | chr8_1:1232638-1232657 | None:intergenic | 35.0% | |
| CACAGTCCTAATAGAAAATC+CGG | - | chr8_1:1232723-1232742 | None:intergenic | 35.0% | |
| GTTTGCTTAATCCTAACAAC+CGG | + | chr8_1:1232834-1232853 | Msa1171550:CDS | 35.0% | |
| TTCACAAACTCAATAACCAG+TGG | - | chr8_1:1232875-1232894 | None:intergenic | 35.0% | |
| GAAAGTGAAAGAAAAACCTG+AGG | + | chr8_1:1232892-1232911 | Msa1171550:CDS | 35.0% | |
| TGAAAGAAAAACCTGAGGAA+AGG | + | chr8_1:1232897-1232916 | Msa1171550:CDS | 35.0% | |
| GAAAGAAAAACCTGAGGAAA+GGG | + | chr8_1:1232898-1232917 | Msa1171550:CDS | 35.0% | |
| TTACTTCTCGAAATGATCTC+AGG | - | chr8_1:1233039-1233058 | None:intergenic | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | + | chr8_1:1233049-1233068 | Msa1171550:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | + | chr8_1:1233111-1233130 | Msa1171550:CDS | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | + | chr8_1:1233118-1233137 | Msa1171550:CDS | 35.0% | |
| GACAATTCATCAAAAGGAGA+TGG | + | chr8_1:1233198-1233217 | Msa1171550:CDS | 35.0% | |
| AATTCATCAAAAGGAGATGG+TGG | + | chr8_1:1233201-1233220 | Msa1171550:CDS | 35.0% | |
| CCATGATTCGTTATTGCTAG+GGG | - | chr8_1:1232636-1232655 | None:intergenic | 40.0% | |
| !! | AGCTGGAATATCTTTGAAGC+TGG | + | chr8_1:1232931-1232950 | Msa1171550:CDS | 40.0% |
| CCATGATTCGTTATTGCTAG+GGG | - | chr8_1:1232636-1232655 | None:intergenic | 40.0% | |
| !! | AGCTGGAATATCTTTGAAGC+TGG | + | chr8_1:1232931-1232950 | Msa1171550:CDS | 40.0% |
| CCCCTAGCAATAACGAATCA+TGG | + | chr8_1:1232633-1232652 | Msa1171550:CDS | 45.0% | |
| GAAAAACCTGAGGAAAGGGT+TGG | + | chr8_1:1232902-1232921 | Msa1171550:CDS | 45.0% | |
| TTACAACCAACCCTTTCCTC+AGG | - | chr8_1:1232911-1232930 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | + | chr8_1:1232914-1232933 | Msa1171550:CDS | 45.0% |
| CTCAGGTAACACAAATGCTC+TGG | - | chr8_1:1233022-1233041 | None:intergenic | 45.0% | |
| GAAGTTATGGGTCAAGGAGT+TGG | + | chr8_1:1233124-1233143 | Msa1171550:CDS | 45.0% | |
| ! | GCACGGAGAATCTTCCTTTT+TGG | + | chr8_1:1233149-1233168 | Msa1171550:CDS | 45.0% |
| ! | CATCAAAAGGAGATGGTGGT+TGG | + | chr8_1:1233205-1233224 | Msa1171550:CDS | 45.0% |
| !! | TGACATCCAAGTTGATGCTG+CGG | + | chr8_1:1233291-1233310 | Msa1171550:CDS | 45.0% |
| CCCCTAGCAATAACGAATCA+TGG | + | chr8_1:1232633-1232652 | Msa1171550:CDS | 45.0% | |
| GAAAAACCTGAGGAAAGGGT+TGG | + | chr8_1:1232902-1232921 | Msa1171550:CDS | 45.0% | |
| TTACAACCAACCCTTTCCTC+AGG | - | chr8_1:1232911-1232930 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | + | chr8_1:1232914-1232933 | Msa1171550:CDS | 45.0% |
| CTCAGGTAACACAAATGCTC+TGG | - | chr8_1:1233022-1233041 | None:intergenic | 45.0% | |
| GAAGTTATGGGTCAAGGAGT+TGG | + | chr8_1:1233124-1233143 | Msa1171550:CDS | 45.0% | |
| ! | GCACGGAGAATCTTCCTTTT+TGG | + | chr8_1:1233149-1233168 | Msa1171550:CDS | 45.0% |
| ! | CATCAAAAGGAGATGGTGGT+TGG | + | chr8_1:1233205-1233224 | Msa1171550:CDS | 45.0% |
| !! | TGACATCCAAGTTGATGCTG+CGG | + | chr8_1:1233291-1233310 | Msa1171550:CDS | 45.0% |
| TCTGATGTTGGCCGGTTGTT+AGG | - | chr8_1:1232848-1232867 | None:intergenic | 50.0% | |
| !! | ACCAGTGGACTGTCTGATGT+TGG | - | chr8_1:1232860-1232879 | None:intergenic | 50.0% |
| GTTATGGGTCAAGGAGTTGG+AGG | + | chr8_1:1233127-1233146 | Msa1171550:CDS | 50.0% | |
| TGTCTTCCGCAGCATCAACT+TGG | - | chr8_1:1233300-1233319 | None:intergenic | 50.0% | |
| TCTGATGTTGGCCGGTTGTT+AGG | - | chr8_1:1232848-1232867 | None:intergenic | 50.0% | |
| !! | ACCAGTGGACTGTCTGATGT+TGG | - | chr8_1:1232860-1232879 | None:intergenic | 50.0% |
| GTTATGGGTCAAGGAGTTGG+AGG | + | chr8_1:1233127-1233146 | Msa1171550:CDS | 50.0% | |
| TGTCTTCCGCAGCATCAACT+TGG | - | chr8_1:1233300-1233319 | None:intergenic | 50.0% | |
| ! | GTGGACTGTCTGATGTTGGC+CGG | - | chr8_1:1232856-1232875 | None:intergenic | 55.0% |
| GCCAACATCAGACAGTCCAC+TGG | + | chr8_1:1232856-1232875 | Msa1171550:CDS | 55.0% | |
| ! | GTGGACTGTCTGATGTTGGC+CGG | - | chr8_1:1232856-1232875 | None:intergenic | 55.0% |
| GCCAACATCAGACAGTCCAC+TGG | + | chr8_1:1232856-1232875 | Msa1171550:CDS | 55.0% | |
| GGGTCAAGGAGTTGGAGGCA+CGG | + | chr8_1:1233132-1233151 | Msa1171550:CDS | 60.0% | |
| GGGTCAAGGAGTTGGAGGCA+CGG | + | chr8_1:1233132-1233151 | Msa1171550:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 1232545 | 1233354 | 1232545 | ID=Msa1171550;Name=Msa1171550 |
| chr8_1 | mRNA | 1232545 | 1233354 | 1232545 | ID=Msa1171550-mRNA-1;Parent=Msa1171550;Name=Msa1171550-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|269 |
| chr8_1 | exon | 1232545 | 1233354 | 1232545 | ID=Msa1171550-mRNA-1:exon:406;Parent=Msa1171550-mRNA-1 |
| chr8_1 | CDS | 1232545 | 1233354 | 1232545 | ID=Msa1171550-mRNA-1:cds;Parent=Msa1171550-mRNA-1 |
| Gene Sequence |
| Protein sequence |
