AlfalfaGEDB | Msa1171660

M. sativa cultivar zhongmu-4 / Msa1171660

KEGG: -
GO: -
PFAM: PF02042.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1171660	sp\|Q9LVU8\|RKD4_ARATH	52.381	105	50	0	191	295	148	252	2.27e-31	120

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1171660	G7J7G0	65.552	299	64	4	1	298	1	261	1.17e-132	386

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TFs/TRs:

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Gene ID	Type	Classification
Msa1171660	TF	RWP-RK

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1171660	MtrunA17_Chr3g0127001	65.552	299	64	4	1	298	1	261	2.26e-136	386
Msa1171660	MtrunA17_Chr1g0182631	64.074	270	60	4	30	298	1	234	3.08e-118	339
Msa1171660	MtrunA17_Chr3g0092141	47.321	112	53	1	191	296	59	170	3.70e-29	110
Msa1171660	MtrunA17_Chr4g0060761	45.370	108	50	1	196	294	160	267	1.65e-27	107
Msa1171660	MtrunA17_Chr5g0406901	37.600	125	60	2	185	291	190	314	5.54e-20	88.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1171660	AT5G53040.1	52.381	105	50	0	191	295	148	252	2.31e-32	120
Msa1171660	AT5G66990.1	49.550	111	45	1	196	295	164	274	1.91e-31	119
Msa1171660	AT1G18790.1	45.161	124	54	2	179	292	120	239	8.70e-28	108
Msa1171660	AT1G74480.1	46.364	110	49	1	189	288	133	242	3.37e-27	107
Msa1171660	AT4G35590.2	34.328	134	68	2	179	292	173	306	1.76e-17	81.6
Msa1171660	AT4G35590.1	34.328	134	68	2	179	292	223	356	2.52e-17	81.6
Msa1171660	AT5G16100.2	37.363	91	49	1	185	267	348	438	6.26e-12	66.2

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Find 56 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCGCTCAAGGCTACAAC+TGG	0.479818	8_1:-1494308	Msa1171660:CDS
AAATGTTGCTTTACCTGAAA+TGG	0.239415	8_1:-1493706	Msa1171660:CDS
AACTACTTTGATCAAGGTAT+TGG	0.343714	8_1:+1493751	None:intergenic
AACTCAATGAAATCATCAAA+TGG	0.477184	8_1:+1493864	None:intergenic
AAGATCATTGAACCACCCAT+TGG	0.537266	8_1:+1493797	None:intergenic
AATGTTGCTTTACCTGAAAT+GGG	0.356567	8_1:-1493705	Msa1171660:CDS
ACATTGAGTGCTGCTGAGAG+TGG	0.594289	8_1:-1493552	Msa1171660:CDS
ACCCAACCTGAAATCGCTCA+AGG	0.612787	8_1:-1494318	Msa1171660:CDS
AGCCTTGAGCGATTTCAGGT+TGG	0.544838	8_1:+1494316	None:intergenic
AGGTTGAAGCTCAAGGGTTG+AGG	0.489859	8_1:+1492826	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATCATGGTTGAAATGAAAC+TGG	+	chr8_1:1492935-1492954	None:intergenic	30.0%
AAAGAATGAACATCGAATCA+TGG	+	chr8_1:1492950-1492969	None:intergenic	30.0%
GGTAAAAAACAGAACAAGTT+AGG	+	chr8_1:1493020-1493039	None:intergenic	30.0%
AACTACTTTGATCAAGGTAT+TGG	+	chr8_1:1493418-1493437	None:intergenic	30.0%
TCTCAAAACTACTTTGATCA+AGG	+	chr8_1:1493424-1493443	None:intergenic	30.0%
TCAGGTAAAGCAACATTTAA+GGG	+	chr8_1:1493458-1493477	None:intergenic	30.0%
TTCAGGTAAAGCAACATTTA+AGG	+	chr8_1:1493459-1493478	None:intergenic	30.0%
TTGTGAACAAACAAGAGAAA+TGG	-	chr8_1:1493567-1493586	Msa1171660:CDS	30.0%
TGTGAACAAACAAGAGAAAT+GGG	-	chr8_1:1493568-1493587	Msa1171660:CDS	30.0%
GTACTAGTATCATAAAGAAG+AGG	-	chr8_1:1493741-1493760	Msa1171660:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	1492836	1494352	1492836	ID=Msa1171660;Name=Msa1171660
chr8_1	mRNA	1492836	1494352	1492836	ID=Msa1171660-mRNA-1;Parent=Msa1171660;Name=Msa1171660-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|0\|0\|1\|1\|1\|3\|0\|298
chr8_1	exon	1494270	1494352	1494270	ID=Msa1171660-mRNA-1:exon:467;Parent=Msa1171660-mRNA-1
chr8_1	exon	1493248	1493902	1493248	ID=Msa1171660-mRNA-1:exon:466;Parent=Msa1171660-mRNA-1
chr8_1	exon	1492836	1492994	1492836	ID=Msa1171660-mRNA-1:exon:465;Parent=Msa1171660-mRNA-1
chr8_1	CDS	1494270	1494352	1494270	ID=Msa1171660-mRNA-1:cds;Parent=Msa1171660-mRNA-1
chr8_1	CDS	1493248	1493902	1493248	ID=Msa1171660-mRNA-1:cds;Parent=Msa1171660-mRNA-1
chr8_1	CDS	1492836	1492994	1492836	ID=Msa1171660-mRNA-1:cds;Parent=Msa1171660-mRNA-1

Gene Sequence

>Msa1171660

ATGGAGTTCTTCACCCAACCTGAAATCGCTCAAGGCTACAACTGGTTTTGCAATGTTCAATCTCAACCAAACAACACAGACAAAATCATGGATATTTCACCATTTGATGATTTCATTGAGTTTGATTGGGCAACTCAGTTTTCATACCAAAGTAATCATTTTTACACCAATGGGTGGTTCAATGATCTTGAAAACTTCAACTTTGATTTCAACCAATACCTTGATCAAAGTAGTTTTGAGATCGATCAAAAGCCCTTAAATGTTGCTTTACCTGAAATGGGGCATTACACTGACAAAGGGAAGAGTATTGTTGAGAGTGGTTTTGTTGAGGCTTCCAAGAAAACAGGAAATCAGTTTGTGAGTTTTGTGAACAAACAAGAGAAATGGGAGAATCAAGAGCCATTGTCATTGACATTGAGTGCTGCTGAGAGTGGATTTGTCGAAGCTTCGGCGAAAACTGAAAGTGGGTTTGTGGATTTTGTGAAGAAAGAGGTTGAAGGGGAAAATCAAGAATTGCCATTACCATTGCCAAGTAGCAGTACTAGTATCATAAAGAAGAGGTCAATTTCATTGCAATTTGATGATATAAGGAAACATTTTGATGTTCCTTTAAAAATGGCTGCTAAGAAAATGAATGTTGGTGTGACCCTTTTGAAGAATAGATGCAGGGAGCTCAACATCACTAGGTGGCCTCATAGAAAATTGAGGAGCTTGACTGTACTCATCGACAACCTTAAGGAAATGGGTTTGGCAAACGAGGTTGCTATGATGGAGAAGAAGAAAAGGATGTTAGAGAAACTTCCTGAAATGGAATTGAATGAAGAAATCAAGAAGTTGAGGCAAGCATGCTTTAAAGCTAAATACAAGAAGAGAAGATTAATTGCCCTCAACCCTTGA

Protein sequence

>Msa1171660

MEFFTQPEIAQGYNWFCNVQSQPNNTDKIMDISPFDDFIEFDWATQFSYQSNHFYTNGWFNDLENFNFDFNQYLDQSSFEIDQKPLNVALPEMGHYTDKGKSIVESGFVEASKKTGNQFVSFVNKQEKWENQEPLSLTLSAAESGFVEASAKTESGFVDFVKKEVEGENQELPLPLPSSSTSIIKKRSISLQFDDIRKHFDVPLKMAAKKMNVGVTLLKNRCRELNITRWPHRKLRSLTVLIDNLKEMGLANEVAMMEKKKRMLEKLPEMELNEEIKKLRQACFKAKYKKRRLIALNP*