AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1174270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1174270 MtrunA17_Chr8g0345671 63.000 400 50 8 1 302 1 400 3.94e-157 444
Msa1174270 MtrunA17_Chr8g0345691 48.449 419 97 12 1 300 1 419 3.71e-110 325
Msa1174270 MtrunA17_Chr8g0345731 60.000 145 42 4 8 136 22 166 1.49e-52 170
Msa1174270 MtrunA17_Chr8g0345711 57.333 150 49 3 1 135 1 150 2.32e-52 169
Msa1174270 MtrunA17_Chr8g0345721 50.857 175 45 2 1 134 26 200 2.65e-51 168
Msa1174270 MtrunA17_Chr8g0345741 58.730 126 40 2 21 134 2 127 1.78e-44 148
Msa1174270 MtrunA17_Chr8g0345701 51.656 151 43 6 10 134 7 153 1.22e-38 134
Msa1174270 MtrunA17_Chr5g0428991 51.880 133 50 4 8 128 23 153 8.43e-35 133
Msa1174270 MtrunA17_Chr3g0128151 52.632 133 49 5 8 128 20 150 1.14e-34 132
Msa1174270 MtrunA17_Chr3g0128111 43.850 187 80 7 13 176 45 229 1.22e-34 126
Msa1174270 MtrunA17_Chr6g0481091 44.737 152 66 4 10 149 6 151 9.73e-34 123
Msa1174270 MtrunA17_Chr2g0309891 48.837 129 50 5 10 125 6 131 3.45e-31 119
Msa1174270 MtrunA17_Chr4g0047841 43.624 149 70 3 6 142 11 157 4.68e-31 119
Msa1174270 MtrunA17_Chr7g0252971 39.877 163 77 4 6 149 4 164 9.75e-31 118
Msa1174270 MtrunA17_Chr5g0433781 49.219 128 51 4 10 125 6 131 6.18e-30 119
Msa1174270 MtrunA17_Chr5g0400461 36.325 234 114 7 10 217 6 230 1.44e-29 114
Msa1174270 MtrunA17_Chr3g0109821 50.000 128 50 4 10 125 6 131 2.37e-29 117
Msa1174270 MtrunA17_Chr2g0318101 46.094 128 55 3 10 125 15 140 2.46e-29 114
Msa1174270 MtrunA17_Chr3g0130731 42.763 152 69 5 10 149 7 152 3.55e-29 112
Msa1174270 MtrunA17_Chr1g0197361 42.945 163 76 4 13 162 11 169 4.24e-29 116
Msa1174270 MtrunA17_Chr2g0316971 47.287 129 54 3 9 125 22 148 1.03e-28 113
Msa1174270 MtrunA17_Chr5g0398971 42.857 140 64 4 3 129 2 138 2.15e-28 112
Msa1174270 MtrunA17_Chr8g0384721 40.789 152 73 4 10 149 7 153 5.00e-28 109
Msa1174270 MtrunA17_Chr4g0018391 39.375 160 74 5 3 149 2 151 5.63e-28 110
Msa1174270 MtrunA17_Chr1g0183741 44.615 130 62 3 10 129 10 139 1.25e-27 108
Msa1174270 MtrunA17_Chr4g0054791 46.269 134 55 4 10 128 6 137 1.59e-27 108
Msa1174270 MtrunA17_Chr6g0484441 46.094 128 53 4 10 125 5 128 1.78e-27 108
Msa1174270 MtrunA17_Chr3g0124761 42.105 152 70 5 10 149 7 152 5.58e-27 107
Msa1174270 MtrunA17_Chr8g0359921 40.789 152 72 5 10 149 14 159 1.10e-26 107
Msa1174270 MtrunA17_Chr7g0214071 44.361 133 63 3 10 133 15 145 2.28e-26 104
Msa1174270 MtrunA17_Chr4g0042561 34.579 214 117 6 10 204 9 218 2.90e-26 104
Msa1174270 MtrunA17_Chr4g0056541 40.000 140 67 4 3 129 2 137 3.02e-26 106
Msa1174270 MtrunA17_Chr4g0060931 45.736 129 54 5 10 126 17 141 3.08e-26 106
Msa1174270 MtrunA17_Chr3g0139331 45.714 140 59 5 10 134 11 148 3.17e-26 105
Msa1174270 MtrunA17_Chr7g0261411 43.077 130 64 2 10 129 10 139 4.16e-26 104
Msa1174270 MtrunA17_Chr8g0361731 40.972 144 71 3 13 144 17 158 4.59e-26 106
Msa1174270 MtrunA17_Chr1g0147421 43.284 134 51 3 10 125 27 153 6.11e-26 105
Msa1174270 MtrunA17_Chr3g0092671 42.353 170 75 6 9 162 4 166 1.18e-25 106
Msa1174270 MtrunA17_Chr2g0318081 45.312 128 54 4 10 125 16 139 1.36e-25 104
Msa1174270 MtrunA17_Chr1g0197351 46.400 125 53 3 13 125 45 167 1.62e-25 105
Msa1174270 MtrunA17_Chr4g0076731 44.776 134 53 5 7 125 51 178 1.64e-25 105
Msa1174270 MtrunA17_Chr3g0091921 45.238 126 57 4 10 125 16 139 1.85e-25 103
Msa1174270 MtrunA17_Chr8g0372121 38.971 136 68 3 10 132 6 139 1.91e-25 103
Msa1174270 MtrunA17_Chr5g0405211 41.538 130 61 3 10 126 8 135 2.48e-25 103
Msa1174270 MtrunA17_Chr4g0017921 43.411 129 58 4 10 125 8 134 2.50e-25 103
Msa1174270 MtrunA17_Chr8g0385991 38.411 151 80 4 10 149 14 162 3.54e-25 100
Msa1174270 MtrunA17_Chr3g0130101 36.158 177 99 3 13 177 10 184 4.05e-25 103
Msa1174270 MtrunA17_Chr6g0453451 45.238 126 57 3 10 125 20 143 4.27e-25 102
Msa1174270 MtrunA17_Chr3g0144271 43.704 135 61 4 10 130 23 156 4.66e-25 103
Msa1174270 MtrunA17_Chr1g0201021 47.619 126 50 4 13 125 22 144 6.57e-25 104
Msa1174270 MtrunA17_Chr8g0346001 40.881 159 72 7 10 155 15 164 6.59e-25 103
Msa1174270 MtrunA17_Chr2g0326361 42.969 128 59 3 11 126 15 140 8.35e-25 101
Msa1174270 MtrunA17_Chr2g0312321 44.186 129 54 4 10 126 11 133 1.03e-24 101
Msa1174270 MtrunA17_Chr7g0263931 41.045 134 63 4 6 125 5 136 1.64e-24 100
Msa1174270 MtrunA17_Chr5g0417001 35.593 177 96 4 13 173 17 191 2.28e-24 101
Msa1174270 MtrunA17_Chr8g0364791 38.000 150 73 6 13 149 9 151 2.91e-24 102
Msa1174270 MtrunA17_Chr7g0253011 41.406 128 63 3 10 125 17 144 2.99e-24 101
Msa1174270 MtrunA17_Chr7g0217721 46.774 124 54 5 12 125 21 142 3.86e-24 100
Msa1174270 MtrunA17_Chr6g0461661 40.157 127 66 2 10 126 18 144 4.48e-24 100
Msa1174270 MtrunA17_Chr6g0453131 38.732 142 76 3 13 144 18 158 5.86e-24 100
Msa1174270 MtrunA17_Chr7g0217711 46.032 126 52 5 12 125 21 142 6.23e-24 95.9
Msa1174270 MtrunA17_Chr4g0038751 41.935 124 61 3 13 125 18 141 7.51e-24 98.6
Msa1174270 MtrunA17_Chr1g0200561 35.294 153 78 4 10 149 19 163 7.77e-24 99.8
Msa1174270 MtrunA17_Chr2g0309331 40.426 141 67 5 10 137 10 146 9.21e-24 99.4
Msa1174270 MtrunA17_Chr5g0417791 42.636 129 58 5 10 126 3 127 2.09e-23 96.7
Msa1174270 MtrunA17_Chr4g0026141 40.909 132 62 3 11 127 6 136 8.99e-23 95.1
Msa1174270 MtrunA17_Chr8g0384201 37.333 150 74 6 13 149 9 151 4.79e-22 95.9
Msa1174270 MtrunA17_Chr2g0321631 39.855 138 66 3 11 132 5 141 1.09e-21 91.7
Msa1174270 MtrunA17_Chr2g0311661 42.424 132 58 5 10 125 20 149 3.15e-21 91.3
Msa1174270 MtrunA17_Chr2g0311651 42.424 132 58 5 10 125 21 150 3.33e-21 91.7
Msa1174270 MtrunA17_Chr2g0321741 55.696 79 31 3 48 125 41 116 4.09e-20 89.4
Msa1174270 MtrunA17_Chr5g0442771 40.476 126 60 4 13 125 21 144 2.25e-18 83.6
Msa1174270 MtrunA17_Chr3g0131911 37.405 131 72 4 10 130 14 144 4.67e-18 81.6
Msa1174270 MtrunA17_Chr7g0266701 33.333 153 82 5 10 143 4 155 1.13e-17 80.9
Msa1174270 MtrunA17_Chr3g0113001 44.444 90 47 3 38 126 2 89 6.49e-17 79.3
Msa1174270 MtrunA17_Chr7g0243371 41.860 86 48 2 43 126 5 90 6.62e-17 75.5
Msa1174270 MtrunA17_Chr4g0050701 30.469 128 67 4 10 125 11 128 4.47e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1174270 AT4G35580.2 54.478 134 47 3 10 131 9 140 4.79e-39 144
Msa1174270 AT4G35580.1 54.478 134 47 3 10 131 9 140 5.18e-39 144
Msa1174270 AT4G35580.3 54.478 134 47 3 10 131 9 140 5.56e-39 144
Msa1174270 AT4G01550.1 51.449 138 53 3 12 135 6 143 2.94e-36 135
Msa1174270 AT1G33060.1 51.908 131 49 3 10 128 24 152 3.66e-36 137
Msa1174270 AT1G33060.2 51.908 131 49 3 10 128 24 152 3.78e-36 137
Msa1174270 AT3G49530.2 48.507 134 56 3 10 131 13 145 9.74e-35 130
Msa1174270 AT3G49530.1 49.254 134 55 4 10 131 13 145 2.78e-34 130
Msa1174270 AT5G17260.1 52.344 128 47 4 10 125 6 131 1.26e-33 128
Msa1174270 AT3G17730.1 49.612 129 51 3 10 126 6 132 3.71e-33 122
Msa1174270 AT5G24590.2 48.507 134 56 4 10 131 13 145 5.20e-33 126
Msa1174270 AT4G01520.1 44.286 140 63 3 11 135 5 144 7.66e-33 123
Msa1174270 AT4G17980.1 44.203 138 65 2 10 135 6 143 2.74e-32 120
Msa1174270 AT3G03200.1 50.781 128 49 4 10 125 6 131 5.05e-32 124
Msa1174270 AT4G17980.2 44.203 138 65 2 10 135 6 143 8.35e-32 120
Msa1174270 AT4G01540.4 44.604 139 62 3 12 135 6 144 1.69e-31 121
Msa1174270 AT4G01540.2 44.604 139 62 3 12 135 6 144 2.25e-31 121
Msa1174270 AT4G01540.3 44.604 139 62 3 12 135 6 144 3.26e-31 121
Msa1174270 AT3G04070.2 47.143 140 57 4 1 125 1 138 3.83e-31 119
Msa1174270 AT4G01540.1 44.604 139 62 3 12 135 6 144 3.90e-31 121
Msa1174270 AT3G04070.1 47.143 140 57 4 1 125 1 138 7.02e-31 119
Msa1174270 AT1G65910.1 51.562 128 48 4 10 125 6 131 1.33e-30 121
Msa1174270 AT3G10480.1 46.565 131 56 3 13 131 30 158 3.85e-30 118
Msa1174270 AT3G10480.2 46.565 131 56 3 13 131 30 158 4.00e-30 118
Msa1174270 AT3G10490.1 38.788 165 87 3 13 165 30 192 7.70e-30 113
Msa1174270 AT5G46590.2 43.421 152 72 4 10 148 6 156 9.44e-30 114
Msa1174270 AT5G46590.1 44.286 140 65 3 10 137 6 144 1.43e-29 114
Msa1174270 AT5G08790.1 38.922 167 85 4 10 164 7 168 1.51e-29 114
Msa1174270 AT1G01720.1 42.105 152 71 4 10 149 7 153 2.23e-29 113
Msa1174270 AT3G10490.2 45.802 131 57 3 13 131 30 158 4.50e-29 115
Msa1174270 AT5G53950.1 48.438 128 56 3 8 125 15 142 5.24e-29 114
Msa1174270 AT1G12260.2 39.375 160 74 5 3 149 2 151 5.25e-29 114
Msa1174270 AT1G62700.1 39.375 160 74 5 3 149 2 151 5.37e-29 114
Msa1174270 AT1G62700.2 39.375 160 74 5 3 149 2 151 5.37e-29 114
Msa1174270 AT1G12260.1 39.375 160 74 5 3 149 2 151 1.09e-28 114
Msa1174270 AT3G15170.1 46.154 130 56 3 8 125 18 145 2.09e-28 111
Msa1174270 AT5G39610.1 44.444 135 63 4 1 125 11 143 7.20e-28 109
Msa1174270 AT1G34190.1 38.122 181 92 6 13 178 19 194 7.49e-28 113
Msa1174270 AT5G66300.1 42.748 131 60 3 10 127 12 140 1.44e-27 108
Msa1174270 AT1G34180.3 40.244 164 79 5 13 162 19 177 1.49e-27 111
Msa1174270 AT1G34180.1 40.244 164 79 5 13 162 19 177 1.67e-27 112
Msa1174270 AT5G61430.1 45.238 126 57 4 10 125 16 139 2.74e-27 108
Msa1174270 AT5G04410.1 43.558 163 75 5 13 162 12 170 3.43e-27 111
Msa1174270 AT1G61110.1 42.667 150 69 5 7 144 13 157 6.34e-27 107
Msa1174270 AT5G63790.1 37.725 167 87 4 10 164 50 211 7.14e-27 107
Msa1174270 AT5G63790.2 37.725 167 87 4 10 164 60 221 7.72e-27 107
Msa1174270 AT5G07680.1 46.032 126 56 4 10 125 17 140 1.14e-26 107
Msa1174270 AT5G07680.2 46.032 126 56 4 10 125 3 126 1.20e-26 107
Msa1174270 AT3G29035.1 44.444 126 58 4 10 125 24 147 1.38e-26 107
Msa1174270 AT1G71930.2 38.562 153 73 4 10 149 9 153 1.46e-26 107
Msa1174270 AT1G71930.1 38.562 153 73 4 10 149 9 153 1.46e-26 107
Msa1174270 AT3G04060.1 46.032 126 57 3 10 125 20 144 1.60e-26 107
Msa1174270 AT3G15500.1 43.056 144 57 5 10 132 14 153 2.25e-26 106
Msa1174270 AT1G69490.1 42.958 142 62 6 10 139 9 143 2.77e-26 105
Msa1174270 AT3G10500.1 38.889 180 88 7 13 176 12 185 2.77e-26 108
Msa1174270 AT1G02230.1 38.636 176 83 7 11 165 4 175 3.44e-26 106
Msa1174270 AT5G62380.2 41.045 134 64 3 9 129 6 137 3.71e-26 106
Msa1174270 AT5G62380.1 41.045 134 64 3 9 129 6 137 3.71e-26 106
Msa1174270 AT1G34180.2 37.500 176 79 5 13 162 19 189 4.23e-26 108
Msa1174270 AT5G09330.5 40.268 149 71 5 13 149 9 151 4.29e-26 107
Msa1174270 AT5G09330.2 40.268 149 71 5 13 149 9 151 4.29e-26 107
Msa1174270 AT5G09330.4 40.268 149 71 5 13 149 9 151 4.29e-26 107
Msa1174270 AT5G09330.3 40.268 149 71 5 13 149 9 151 4.29e-26 107
Msa1174270 AT5G09330.1 40.268 149 71 5 13 149 9 151 4.29e-26 107
Msa1174270 AT1G34180.4 37.500 176 79 5 13 162 19 189 4.55e-26 107
Msa1174270 AT3G18400.1 46.032 126 56 3 10 125 5 128 4.57e-26 105
Msa1174270 AT3G15510.1 45.968 124 53 3 14 125 21 142 4.70e-26 106
Msa1174270 AT4G10350.1 42.029 138 65 4 10 134 9 144 5.55e-26 105
Msa1174270 AT1G52890.1 45.312 128 54 4 10 125 14 137 7.00e-26 105
Msa1174270 AT2G24430.2 39.474 152 73 4 10 149 16 160 7.71e-26 104
Msa1174270 AT2G24430.1 39.474 152 73 4 10 149 16 160 7.71e-26 104
Msa1174270 AT1G54330.1 47.328 131 55 4 10 128 6 134 9.35e-26 104
Msa1174270 AT3G10480.3 37.805 164 55 4 13 131 30 191 1.06e-25 107
Msa1174270 AT1G76420.1 42.857 126 62 3 10 125 22 147 1.13e-25 104
Msa1174270 AT2G33480.1 48.413 126 52 5 10 126 15 136 1.55e-25 102
Msa1174270 AT2G33480.2 48.413 126 52 5 10 126 15 136 1.64e-25 102
Msa1174270 AT5G04395.1 37.580 157 74 4 1 133 19 175 2.27e-25 100
Msa1174270 AT1G32510.1 36.842 171 88 4 8 161 4 171 2.64e-25 102
Msa1174270 AT2G18060.3 41.538 130 61 3 10 126 9 136 2.78e-25 103
Msa1174270 AT2G18060.1 41.538 130 61 3 10 126 9 136 2.78e-25 103
Msa1174270 AT5G39820.1 42.537 134 63 3 13 134 23 154 3.38e-25 103
Msa1174270 AT2G17040.1 44.094 127 56 4 13 126 9 133 4.66e-25 102
Msa1174270 AT5G18270.2 45.238 126 58 3 10 125 21 145 4.97e-25 102
Msa1174270 AT1G33280.1 40.876 137 66 4 10 133 8 142 5.08e-25 102
Msa1174270 AT4G27410.2 43.750 128 56 4 10 125 14 137 5.17e-25 102
Msa1174270 AT1G26870.1 44.800 125 55 3 13 125 19 141 7.35e-25 103
Msa1174270 AT5G64060.1 35.882 170 87 7 8 162 5 167 7.85e-25 102
Msa1174270 AT4G36160.2 41.085 129 61 3 10 125 14 140 9.69e-25 102
Msa1174270 AT4G36160.1 41.085 129 61 3 10 125 10 136 9.82e-25 102
Msa1174270 AT4G36160.3 41.085 129 61 3 10 125 10 136 9.82e-25 102
Msa1174270 AT2G46770.1 42.336 137 61 5 10 133 16 147 1.33e-24 102
Msa1174270 AT3G44290.2 43.066 137 57 6 1 125 11 138 1.42e-24 99.4
Msa1174270 AT5G18270.1 44.444 126 59 3 10 125 21 145 1.66e-24 101
Msa1174270 AT5G13180.1 42.063 126 62 3 10 126 14 137 2.48e-24 99.4
Msa1174270 AT1G77450.1 39.073 151 74 5 11 149 11 155 5.87e-24 98.2
Msa1174270 AT4G28530.1 38.732 142 61 3 10 125 10 151 6.20e-24 100
Msa1174270 AT2G02450.1 42.958 142 58 6 13 137 54 189 6.56e-24 100
Msa1174270 AT2G02450.2 43.262 141 59 6 13 137 54 189 8.79e-24 100
Msa1174270 AT5G22290.1 43.284 134 59 6 13 132 24 154 9.27e-24 99.8
Msa1174270 AT3G44290.1 43.066 137 57 6 1 125 11 138 1.57e-23 99.0
Msa1174270 AT3G61910.1 36.478 159 83 5 10 155 11 164 3.78e-23 97.8
Msa1174270 AT3G12910.1 43.182 132 63 3 13 132 24 155 4.48e-23 97.1
Msa1174270 AT1G79580.3 41.085 129 62 4 10 125 17 144 7.35e-23 97.4
Msa1174270 AT1G79580.5 41.085 129 62 4 10 125 17 144 7.35e-23 97.4
Msa1174270 AT1G79580.4 41.085 129 62 4 10 125 17 144 7.35e-23 97.4
Msa1174270 AT1G79580.2 41.085 129 62 4 10 125 17 144 7.35e-23 97.4
Msa1174270 AT1G79580.1 41.085 129 62 4 10 125 17 144 7.35e-23 97.4
Msa1174270 AT4G27410.3 38.889 144 59 4 10 126 14 155 1.85e-22 95.5
Msa1174270 AT2G27300.1 40.800 125 59 4 13 125 17 138 2.55e-22 95.5
Msa1174270 AT1G56010.2 41.732 127 61 4 10 125 19 143 4.85e-22 94.7
Msa1174270 AT1G32770.1 40.310 129 62 3 10 125 16 142 9.54e-22 94.4
Msa1174270 AT1G02250.1 30.651 261 141 10 11 240 4 255 2.05e-21 93.2
Msa1174270 AT1G52880.1 41.026 156 63 6 14 155 21 161 2.47e-21 92.4
Msa1174270 AT3G12977.1 41.406 128 59 5 10 125 18 141 3.53e-21 91.3
Msa1174270 AT5G22380.1 38.406 138 62 4 10 128 5 138 4.09e-21 90.1
Msa1174270 AT2G43000.1 40.972 144 65 6 13 140 21 160 6.24e-21 90.5
Msa1174270 AT3G04420.2 38.931 131 64 3 11 127 4 132 9.77e-20 87.8
Msa1174270 AT3G04420.1 38.931 131 64 3 11 127 4 132 1.03e-19 88.2
Msa1174270 AT3G44350.1 35.606 132 69 3 12 128 7 137 8.99e-19 82.0
Msa1174270 AT3G44350.2 35.606 132 69 3 12 128 7 137 2.39e-18 82.8
Msa1174270 AT1G32870.1 44.000 125 54 4 13 125 13 133 2.48e-18 85.5
Msa1174270 AT1G32870.2 44.000 125 54 4 13 125 13 133 2.50e-18 85.5
Msa1174270 AT1G32870.3 44.000 125 54 4 13 125 49 169 2.71e-18 85.5
Msa1174270 AT1G01010.1 37.931 145 68 4 12 136 5 147 2.99e-18 85.1
Msa1174270 AT5G61430.2 51.899 79 35 3 49 126 1 77 3.31e-18 83.2
Msa1174270 AT4G10350.2 46.067 89 45 2 47 134 11 97 2.25e-17 80.9
Msa1174270 AT1G56010.1 52.113 71 31 2 56 125 8 76 5.01e-17 79.3
Msa1174270 AT3G12977.2 50.704 71 32 2 56 125 20 88 6.60e-17 78.6
Msa1174270 AT2G33480.3 52.703 74 32 2 54 126 8 79 1.18e-16 77.4
Msa1174270 AT1G02220.1 38.686 137 65 7 11 134 4 134 4.17e-16 78.6
Msa1174270 AT4G28530.2 39.252 107 40 2 10 91 10 116 2.86e-14 72.4
Msa1174270 AT3G56530.1 32.680 153 80 6 11 149 53 196 3.53e-13 69.3
Msa1174270 AT3G04430.1 35.556 135 61 6 16 127 6 137 2.98e-12 65.1
Msa1174270 AT5G64530.2 30.709 127 64 4 10 125 3 116 5.37e-11 60.5
Msa1174270 AT5G64530.1 30.709 127 64 4 10 125 3 116 6.50e-11 60.8

Find 49 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCATTTCGAGATGATTTC+GGG 0.227385 8_1:+5350886 Msa1174270:CDS
AGAGGTACTGAAAATGTTAT+TGG 0.269464 8_1:+5349969 Msa1174270:CDS
ACAAATACGCAAATAGTAAA+AGG 0.269504 8_1:+5349883 Msa1174270:CDS
GCATCATTTCGAGATGATTT+CGG 0.275502 8_1:+5350885 Msa1174270:CDS
TGGTCTTGACCGTAAGATTA+AGG 0.284539 8_1:+5349944 Msa1174270:CDS
CTCCTGGTGTTAATAGCAAC+TGG 0.300575 8_1:+5350027 Msa1174270:CDS
TACTATAATGGTTGTGCTCC+TGG 0.345726 8_1:+5350011 Msa1174270:CDS
AAAACACTTGTGTACTATAA+TGG 0.357000 8_1:+5349999 Msa1174270:CDS
GACATGGTGGTGGTTGGTCT+TGG 0.361614 8_1:-5351014 None:intergenic
CATATCGATGTGTCCACTTT+CGG 0.387067 8_1:-5350537 None:intergenic
TCGTTGAGAATGATTTACTA+TGG 0.400930 8_1:+5350685 Msa1174270:CDS
GAGGTACTGAAAATGTTATT+GGG 0.407217 8_1:+5349970 Msa1174270:CDS
GAAGGACACAGTGTGGATAC+TGG 0.428296 8_1:+5349913 Msa1174270:CDS
GACCGTAAGATTAAGGTTAG+AGG 0.442904 8_1:+5349951 Msa1174270:CDS
AATGTTTCAGGAACCTCATC+AGG 0.458637 8_1:+5350427 Msa1174270:intron
ATTGAAGACTGGTATGGAAA+AGG 0.465871 8_1:-5350464 None:intergenic
TCATTCTCAACGACTCGGAC+TGG 0.469733 8_1:-5350675 None:intergenic
CCTCATAACTATGTTCCTGT+TGG 0.475870 8_1:+5349608 Msa1174270:CDS
TTTACAAGCTCTTCATCTGT+TGG 0.494683 8_1:-5349644 None:intergenic
GGACGGAATCTGAATCCAAC+AGG 0.495067 8_1:-5349623 None:intergenic
ATTGTTGAACATCCTAGTTC+AGG 0.495441 8_1:+5350852 Msa1174270:CDS
TACCTCTAACCTTAATCTTA+CGG 0.495515 8_1:-5349953 None:intergenic
TTCAATCTCTGAAAATGAGA+TGG 0.499943 8_1:+5350481 Msa1174270:CDS
TCAGAGATTGAAGACTGGTA+TGG 0.501687 8_1:-5350470 None:intergenic
CCAACAGGAACATAGTTATG+AGG 0.518146 8_1:-5349608 None:intergenic
GATAGAATGATATGTGATGA+AGG 0.532647 8_1:+5350243 Msa1174270:CDS
GAAGTATCTTTATCGAACTC+AGG 0.533769 8_1:+5350503 Msa1174270:CDS
CTCAACGACTCGGACTGGAC+AGG 0.555897 8_1:-5350670 None:intergenic
CAAGCTCTTCATCTGTTGGA+CGG 0.560584 8_1:-5349640 None:intergenic
ACAAACCTGGTTATCATCAA+AGG 0.564326 8_1:-5350075 None:intergenic
TACCACCTTTGATGATAACC+AGG 0.567592 8_1:+5350070 Msa1174270:CDS
TAGAATGATATGTGATGAAG+GGG 0.574647 8_1:+5350245 Msa1174270:CDS
ATAGTAAAAGGTTTAACAGA+AGG 0.579914 8_1:+5349895 Msa1174270:CDS
TCCTGGTGTTAATAGCAACT+GGG 0.584943 8_1:+5350028 Msa1174270:CDS
TACTAGAATGGATACTTCAA+CGG 0.588354 8_1:+5350828 Msa1174270:intron
ATAGAATGATATGTGATGAA+GGG 0.600020 8_1:+5350244 Msa1174270:CDS
TGTGGATACTGGAAAGCCAC+TGG 0.602459 8_1:+5349924 Msa1174270:CDS
TTGCAATTGATCATCTCTAG+AGG 0.607895 8_1:-5350948 None:intergenic
AATCTTACGGTCAAGACCAG+TGG 0.626820 8_1:-5349940 None:intergenic
ACCCAGTTGCTATTAACACC+AGG 0.638889 8_1:-5350029 None:intergenic
TAAAATCCGATGATCCCGAA+TGG 0.649145 8_1:+5349838 Msa1174270:CDS
ATCATTTCGAGATGATTTCG+GGG 0.650121 8_1:+5350887 Msa1174270:CDS
GTAAATCATTCTCAACGACT+CGG 0.660341 8_1:-5350680 None:intergenic
AACCTGGTTATCATCAAAGG+TGG 0.663139 8_1:-5350072 None:intergenic
AGCTATAACATTGTTGACAG+TGG 0.663788 8_1:-5349702 None:intergenic
TCATTTCGAGATGATTTCGG+GGG 0.671935 8_1:+5350888 Msa1174270:CDS
TTTGCGTATTTGTAATCACG+AGG 0.676971 8_1:-5349873 None:intergenic
TTTAACAGAAGGACACAGTG+TGG 0.694289 8_1:+5349906 Msa1174270:CDS
AGAATGATATGTGATGAAGG+GGG 0.821696 8_1:+5350246 Msa1174270:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATCAAAGAAATATTTA+CGG - chr8_1:5350746-5350765 None:intergenic 10.0%
!! ATATATCAAAGAAATATTTA+CGG - chr8_1:5350746-5350765 None:intergenic 10.0%
!!! TTTTATGAGAAAATAATGAT+GGG - chr8_1:5349771-5349790 None:intergenic 15.0%
!!! TTTTTATGAGAAAATAATGA+TGG - chr8_1:5349772-5349791 None:intergenic 15.0%
!! TATATCAAAGAAATATTTAC+GGG - chr8_1:5350745-5350764 None:intergenic 15.0%
!!! TTTTATGAGAAAATAATGAT+GGG - chr8_1:5349771-5349790 None:intergenic 15.0%
!!! TTTTTATGAGAAAATAATGA+TGG - chr8_1:5349772-5349791 None:intergenic 15.0%
!! TATATCAAAGAAATATTTAC+GGG - chr8_1:5350745-5350764 None:intergenic 15.0%
!! TTTATGAGAAAATAATGATG+GGG - chr8_1:5349770-5349789 None:intergenic 20.0%
!!! TGTTTAACTTTATGAGTATA+TGG + chr8_1:5350111-5350130 Msa1174270:intron 20.0%
!! ATCAATTCAATGTTAAATGA+CGG + chr8_1:5350598-5350617 Msa1174270:intron 20.0%
!! TTTATGAGAAAATAATGATG+GGG - chr8_1:5349770-5349789 None:intergenic 20.0%
!!! TGTTTAACTTTATGAGTATA+TGG + chr8_1:5350111-5350130 Msa1174270:intron 20.0%
!! ATCAATTCAATGTTAAATGA+CGG + chr8_1:5350598-5350617 Msa1174270:intron 20.0%
! TATCTCAAAAACAAGTTACT+TGG + chr8_1:5349671-5349690 Msa1174270:CDS 25.0%
! ACAAATACGCAAATAGTAAA+AGG + chr8_1:5349883-5349902 Msa1174270:CDS 25.0%
! ATAGTAAAAGGTTTAACAGA+AGG + chr8_1:5349895-5349914 Msa1174270:CDS 25.0%
! AAAACACTTGTGTACTATAA+TGG + chr8_1:5349999-5350018 Msa1174270:CDS 25.0%
!!! GTATATGGTATGAGTTTTTT+CGG + chr8_1:5350126-5350145 Msa1174270:intron 25.0%
! ATAGAATGATATGTGATGAA+GGG + chr8_1:5350244-5350263 Msa1174270:CDS 25.0%
!!! TCTTTGAAATACTACTAGAA+TGG + chr8_1:5350816-5350835 Msa1174270:intron 25.0%
! TATCTCAAAAACAAGTTACT+TGG + chr8_1:5349671-5349690 Msa1174270:CDS 25.0%
! ACAAATACGCAAATAGTAAA+AGG + chr8_1:5349883-5349902 Msa1174270:CDS 25.0%
! ATAGTAAAAGGTTTAACAGA+AGG + chr8_1:5349895-5349914 Msa1174270:CDS 25.0%
! AAAACACTTGTGTACTATAA+TGG + chr8_1:5349999-5350018 Msa1174270:CDS 25.0%
!!! GTATATGGTATGAGTTTTTT+CGG + chr8_1:5350126-5350145 Msa1174270:intron 25.0%
! ATAGAATGATATGTGATGAA+GGG + chr8_1:5350244-5350263 Msa1174270:CDS 25.0%
!!! TCTTTGAAATACTACTAGAA+TGG + chr8_1:5350816-5350835 Msa1174270:intron 25.0%
!!! GTAACTTGTTTTTGAGATAG+TGG - chr8_1:5349670-5349689 None:intergenic 30.0%
AGGACTAAAGAAAAACCATT+CGG - chr8_1:5349856-5349875 None:intergenic 30.0%
TACCTCTAACCTTAATCTTA+CGG - chr8_1:5349956-5349975 None:intergenic 30.0%
!! AGAGGTACTGAAAATGTTAT+TGG + chr8_1:5349969-5349988 Msa1174270:CDS 30.0%
!! GAGGTACTGAAAATGTTATT+GGG + chr8_1:5349970-5349989 Msa1174270:CDS 30.0%
TTGTTGTAACAAAACAAACC+TGG - chr8_1:5350091-5350110 None:intergenic 30.0%
! AAAGTCCTCTACGAAAATTT+TGG - chr8_1:5350171-5350190 None:intergenic 30.0%
!!! TTCCTCTTCAGTTTTTTTCT+CGG - chr8_1:5350220-5350239 None:intergenic 30.0%
GATAGAATGATATGTGATGA+AGG + chr8_1:5350243-5350262 Msa1174270:CDS 30.0%
TAGAATGATATGTGATGAAG+GGG + chr8_1:5350245-5350264 Msa1174270:CDS 30.0%
! TGTTTCATGATTTTCGTGAT+CGG - chr8_1:5350291-5350310 None:intergenic 30.0%
GAAAATCATGAAACAGTTGA+AGG + chr8_1:5350296-5350315 Msa1174270:intron 30.0%
AAGAATGAAATCCTTACATC+AGG - chr8_1:5350326-5350345 None:intergenic 30.0%
TAAGCATGTTACATGTAAGA+AGG - chr8_1:5350394-5350413 None:intergenic 30.0%
TTCTTACATGTAACATGCTT+AGG + chr8_1:5350393-5350412 Msa1174270:intron 30.0%
ATGTAACATGCTTAGGAATA+TGG + chr8_1:5350400-5350419 Msa1174270:intron 30.0%
!!! TGGAAAAGGAAAGTACTTTT+CGG - chr8_1:5350453-5350472 None:intergenic 30.0%
! TTCAATCTCTGAAAATGAGA+TGG + chr8_1:5350481-5350500 Msa1174270:CDS 30.0%
TTCCTCTTCATAGTTTCAAA+CGG - chr8_1:5350569-5350588 None:intergenic 30.0%
TCGTTGAGAATGATTTACTA+TGG + chr8_1:5350685-5350704 Msa1174270:CDS 30.0%
! TCTTTTATTCAGTACTGTGA+TGG + chr8_1:5350777-5350796 Msa1174270:intron 30.0%
TACTAGAATGGATACTTCAA+CGG + chr8_1:5350828-5350847 Msa1174270:intron 30.0%
!!! GTAACTTGTTTTTGAGATAG+TGG - chr8_1:5349670-5349689 None:intergenic 30.0%
AGGACTAAAGAAAAACCATT+CGG - chr8_1:5349856-5349875 None:intergenic 30.0%
TACCTCTAACCTTAATCTTA+CGG - chr8_1:5349956-5349975 None:intergenic 30.0%
!! AGAGGTACTGAAAATGTTAT+TGG + chr8_1:5349969-5349988 Msa1174270:CDS 30.0%
!! GAGGTACTGAAAATGTTATT+GGG + chr8_1:5349970-5349989 Msa1174270:CDS 30.0%
TTGTTGTAACAAAACAAACC+TGG - chr8_1:5350091-5350110 None:intergenic 30.0%
! AAAGTCCTCTACGAAAATTT+TGG - chr8_1:5350171-5350190 None:intergenic 30.0%
!!! TTCCTCTTCAGTTTTTTTCT+CGG - chr8_1:5350220-5350239 None:intergenic 30.0%
GATAGAATGATATGTGATGA+AGG + chr8_1:5350243-5350262 Msa1174270:CDS 30.0%
TAGAATGATATGTGATGAAG+GGG + chr8_1:5350245-5350264 Msa1174270:CDS 30.0%
! TGTTTCATGATTTTCGTGAT+CGG - chr8_1:5350291-5350310 None:intergenic 30.0%
GAAAATCATGAAACAGTTGA+AGG + chr8_1:5350296-5350315 Msa1174270:intron 30.0%
AAGAATGAAATCCTTACATC+AGG - chr8_1:5350326-5350345 None:intergenic 30.0%
TAAGCATGTTACATGTAAGA+AGG - chr8_1:5350394-5350413 None:intergenic 30.0%
TTCTTACATGTAACATGCTT+AGG + chr8_1:5350393-5350412 Msa1174270:intron 30.0%
ATGTAACATGCTTAGGAATA+TGG + chr8_1:5350400-5350419 Msa1174270:intron 30.0%
!!! TGGAAAAGGAAAGTACTTTT+CGG - chr8_1:5350453-5350472 None:intergenic 30.0%
! TTCAATCTCTGAAAATGAGA+TGG + chr8_1:5350481-5350500 Msa1174270:CDS 30.0%
TTCCTCTTCATAGTTTCAAA+CGG - chr8_1:5350569-5350588 None:intergenic 30.0%
TCGTTGAGAATGATTTACTA+TGG + chr8_1:5350685-5350704 Msa1174270:CDS 30.0%
! TCTTTTATTCAGTACTGTGA+TGG + chr8_1:5350777-5350796 Msa1174270:intron 30.0%
TACTAGAATGGATACTTCAA+CGG + chr8_1:5350828-5350847 Msa1174270:intron 30.0%
TTTACAAGCTCTTCATCTGT+TGG - chr8_1:5349647-5349666 None:intergenic 35.0%
AGCTATAACATTGTTGACAG+TGG - chr8_1:5349705-5349724 None:intergenic 35.0%
!!! GGGTTTGTTTGTTTTTTACC+TGG - chr8_1:5349750-5349769 None:intergenic 35.0%
GGACTAAAGAAAAACCATTC+GGG - chr8_1:5349855-5349874 None:intergenic 35.0%
TTTGCGTATTTGTAATCACG+AGG - chr8_1:5349876-5349895 None:intergenic 35.0%
ACAAACCTGGTTATCATCAA+AGG - chr8_1:5350078-5350097 None:intergenic 35.0%
! ATCCTCCAAAATTTTCGTAG+AGG + chr8_1:5350163-5350182 Msa1174270:intron 35.0%
AGAATGATATGTGATGAAGG+GGG + chr8_1:5350246-5350265 Msa1174270:CDS 35.0%
TTGAAGGAATTCCTGATGTA+AGG + chr8_1:5350312-5350331 Msa1174270:intron 35.0%
GAATATGGAACCAATGTTTC+AGG + chr8_1:5350415-5350434 Msa1174270:intron 35.0%
ATTGAAGACTGGTATGGAAA+AGG - chr8_1:5350467-5350486 None:intergenic 35.0%
! CATTTTCAGAGATTGAAGAC+TGG - chr8_1:5350478-5350497 None:intergenic 35.0%
! GAAGTATCTTTATCGAACTC+AGG + chr8_1:5350503-5350522 Msa1174270:CDS 35.0%
! GGATTTTATGATTCCGAAAG+TGG + chr8_1:5350524-5350543 Msa1174270:CDS 35.0%
GTAAATCATTCTCAACGACT+CGG - chr8_1:5350683-5350702 None:intergenic 35.0%
TTATTCAGTACTGTGATGGT+TGG + chr8_1:5350781-5350800 Msa1174270:intron 35.0%
ATTGTTGAACATCCTAGTTC+AGG + chr8_1:5350852-5350871 Msa1174270:CDS 35.0%
GCATCATTTCGAGATGATTT+CGG + chr8_1:5350885-5350904 Msa1174270:CDS 35.0%
CATCATTTCGAGATGATTTC+GGG + chr8_1:5350886-5350905 Msa1174270:CDS 35.0%
ATCATTTCGAGATGATTTCG+GGG + chr8_1:5350887-5350906 Msa1174270:CDS 35.0%
TTGCAATTGATCATCTCTAG+AGG - chr8_1:5350951-5350970 None:intergenic 35.0%
! TGAAGCAGATCTTTTTGACA+TGG - chr8_1:5351033-5351052 None:intergenic 35.0%
TTTACAAGCTCTTCATCTGT+TGG - chr8_1:5349647-5349666 None:intergenic 35.0%
AGCTATAACATTGTTGACAG+TGG - chr8_1:5349705-5349724 None:intergenic 35.0%
!!! GGGTTTGTTTGTTTTTTACC+TGG - chr8_1:5349750-5349769 None:intergenic 35.0%
GGACTAAAGAAAAACCATTC+GGG - chr8_1:5349855-5349874 None:intergenic 35.0%
TTTGCGTATTTGTAATCACG+AGG - chr8_1:5349876-5349895 None:intergenic 35.0%
ACAAACCTGGTTATCATCAA+AGG - chr8_1:5350078-5350097 None:intergenic 35.0%
! ATCCTCCAAAATTTTCGTAG+AGG + chr8_1:5350163-5350182 Msa1174270:intron 35.0%
AGAATGATATGTGATGAAGG+GGG + chr8_1:5350246-5350265 Msa1174270:CDS 35.0%
TTGAAGGAATTCCTGATGTA+AGG + chr8_1:5350312-5350331 Msa1174270:intron 35.0%
GAATATGGAACCAATGTTTC+AGG + chr8_1:5350415-5350434 Msa1174270:intron 35.0%
ATTGAAGACTGGTATGGAAA+AGG - chr8_1:5350467-5350486 None:intergenic 35.0%
! CATTTTCAGAGATTGAAGAC+TGG - chr8_1:5350478-5350497 None:intergenic 35.0%
! GAAGTATCTTTATCGAACTC+AGG + chr8_1:5350503-5350522 Msa1174270:CDS 35.0%
! GGATTTTATGATTCCGAAAG+TGG + chr8_1:5350524-5350543 Msa1174270:CDS 35.0%
GTAAATCATTCTCAACGACT+CGG - chr8_1:5350683-5350702 None:intergenic 35.0%
TTATTCAGTACTGTGATGGT+TGG + chr8_1:5350781-5350800 Msa1174270:intron 35.0%
ATTGTTGAACATCCTAGTTC+AGG + chr8_1:5350852-5350871 Msa1174270:CDS 35.0%
GCATCATTTCGAGATGATTT+CGG + chr8_1:5350885-5350904 Msa1174270:CDS 35.0%
CATCATTTCGAGATGATTTC+GGG + chr8_1:5350886-5350905 Msa1174270:CDS 35.0%
ATCATTTCGAGATGATTTCG+GGG + chr8_1:5350887-5350906 Msa1174270:CDS 35.0%
TTGCAATTGATCATCTCTAG+AGG - chr8_1:5350951-5350970 None:intergenic 35.0%
! TGAAGCAGATCTTTTTGACA+TGG - chr8_1:5351033-5351052 None:intergenic 35.0%
CCAACAGGAACATAGTTATG+AGG - chr8_1:5349611-5349630 None:intergenic 40.0%
CCTCATAACTATGTTCCTGT+TGG + chr8_1:5349608-5349627 Msa1174270:CDS 40.0%
! TATAGCTGCAAGTTGAACCT+TGG + chr8_1:5349718-5349737 Msa1174270:intron 40.0%
! ATAGCTGCAAGTTGAACCTT+GGG + chr8_1:5349719-5349738 Msa1174270:intron 40.0%
! TTTTTACCTGGTAAGTCCCA+AGG - chr8_1:5349738-5349757 None:intergenic 40.0%
TAAAATCCGATGATCCCGAA+TGG + chr8_1:5349838-5349857 Msa1174270:CDS 40.0%
GAAAAACCATTCGGGATCAT+CGG - chr8_1:5349847-5349866 None:intergenic 40.0%
TTTAACAGAAGGACACAGTG+TGG + chr8_1:5349906-5349925 Msa1174270:CDS 40.0%
! TGGTCTTGACCGTAAGATTA+AGG + chr8_1:5349944-5349963 Msa1174270:CDS 40.0%
GACCGTAAGATTAAGGTTAG+AGG + chr8_1:5349951-5349970 Msa1174270:CDS 40.0%
TACTATAATGGTTGTGCTCC+TGG + chr8_1:5350011-5350030 Msa1174270:CDS 40.0%
! TCCTGGTGTTAATAGCAACT+GGG + chr8_1:5350028-5350047 Msa1174270:CDS 40.0%
! TACCACCTTTGATGATAACC+AGG + chr8_1:5350070-5350089 Msa1174270:CDS 40.0%
AACCTGGTTATCATCAAAGG+TGG - chr8_1:5350075-5350094 None:intergenic 40.0%
! GTCCTCTACGAAAATTTTGG+AGG - chr8_1:5350168-5350187 None:intergenic 40.0%
AGCCGAGAAAAAAACTGAAG+AGG + chr8_1:5350215-5350234 Msa1174270:CDS 40.0%
TGATGAGGTTCCTGAAACAT+TGG - chr8_1:5350428-5350447 None:intergenic 40.0%
AATGTTTCAGGAACCTCATC+AGG + chr8_1:5350427-5350446 Msa1174270:intron 40.0%
TCAGAGATTGAAGACTGGTA+TGG - chr8_1:5350473-5350492 None:intergenic 40.0%
CATATCGATGTGTCCACTTT+CGG - chr8_1:5350540-5350559 None:intergenic 40.0%
! GTCCGTTTGAAACTATGAAG+AGG + chr8_1:5350564-5350583 Msa1174270:intron 40.0%
TCATTTCGAGATGATTTCGG+GGG + chr8_1:5350888-5350907 Msa1174270:CDS 40.0%
! AGATCTTTTTGACATGGTGG+TGG - chr8_1:5351027-5351046 None:intergenic 40.0%
! AGCAGATCTTTTTGACATGG+TGG - chr8_1:5351030-5351049 None:intergenic 40.0%
CCAACAGGAACATAGTTATG+AGG - chr8_1:5349611-5349630 None:intergenic 40.0%
CCTCATAACTATGTTCCTGT+TGG + chr8_1:5349608-5349627 Msa1174270:CDS 40.0%
! TATAGCTGCAAGTTGAACCT+TGG + chr8_1:5349718-5349737 Msa1174270:intron 40.0%
! ATAGCTGCAAGTTGAACCTT+GGG + chr8_1:5349719-5349738 Msa1174270:intron 40.0%
! TTTTTACCTGGTAAGTCCCA+AGG - chr8_1:5349738-5349757 None:intergenic 40.0%
TAAAATCCGATGATCCCGAA+TGG + chr8_1:5349838-5349857 Msa1174270:CDS 40.0%
GAAAAACCATTCGGGATCAT+CGG - chr8_1:5349847-5349866 None:intergenic 40.0%
TTTAACAGAAGGACACAGTG+TGG + chr8_1:5349906-5349925 Msa1174270:CDS 40.0%
! TGGTCTTGACCGTAAGATTA+AGG + chr8_1:5349944-5349963 Msa1174270:CDS 40.0%
GACCGTAAGATTAAGGTTAG+AGG + chr8_1:5349951-5349970 Msa1174270:CDS 40.0%
TACTATAATGGTTGTGCTCC+TGG + chr8_1:5350011-5350030 Msa1174270:CDS 40.0%
! TCCTGGTGTTAATAGCAACT+GGG + chr8_1:5350028-5350047 Msa1174270:CDS 40.0%
! TACCACCTTTGATGATAACC+AGG + chr8_1:5350070-5350089 Msa1174270:CDS 40.0%
AACCTGGTTATCATCAAAGG+TGG - chr8_1:5350075-5350094 None:intergenic 40.0%
! GTCCTCTACGAAAATTTTGG+AGG - chr8_1:5350168-5350187 None:intergenic 40.0%
AGCCGAGAAAAAAACTGAAG+AGG + chr8_1:5350215-5350234 Msa1174270:CDS 40.0%
TGATGAGGTTCCTGAAACAT+TGG - chr8_1:5350428-5350447 None:intergenic 40.0%
AATGTTTCAGGAACCTCATC+AGG + chr8_1:5350427-5350446 Msa1174270:intron 40.0%
TCAGAGATTGAAGACTGGTA+TGG - chr8_1:5350473-5350492 None:intergenic 40.0%
CATATCGATGTGTCCACTTT+CGG - chr8_1:5350540-5350559 None:intergenic 40.0%
! GTCCGTTTGAAACTATGAAG+AGG + chr8_1:5350564-5350583 Msa1174270:intron 40.0%
TCATTTCGAGATGATTTCGG+GGG + chr8_1:5350888-5350907 Msa1174270:CDS 40.0%
! AGATCTTTTTGACATGGTGG+TGG - chr8_1:5351027-5351046 None:intergenic 40.0%
! AGCAGATCTTTTTGACATGG+TGG - chr8_1:5351030-5351049 None:intergenic 40.0%
CAAGCTCTTCATCTGTTGGA+CGG - chr8_1:5349643-5349662 None:intergenic 45.0%
AATCTTACGGTCAAGACCAG+TGG - chr8_1:5349943-5349962 None:intergenic 45.0%
! CTCCTGGTGTTAATAGCAAC+TGG + chr8_1:5350027-5350046 Msa1174270:CDS 45.0%
! ACCCAGTTGCTATTAACACC+AGG - chr8_1:5350032-5350051 None:intergenic 45.0%
TCGGAACTCATGTGTCTACT+TGG - chr8_1:5350272-5350291 None:intergenic 45.0%
! AAGTACTTTTCGGCCTGATG+AGG - chr8_1:5350443-5350462 None:intergenic 45.0%
GCATAGTATTCGCCTGAACT+AGG - chr8_1:5350867-5350886 None:intergenic 45.0%
AGATGATTTCGGGGGATTTG+AGG + chr8_1:5350896-5350915 Msa1174270:CDS 45.0%
!!! CTTTTTGACATGGTGGTGGT+TGG - chr8_1:5351023-5351042 None:intergenic 45.0%
CAAGCTCTTCATCTGTTGGA+CGG - chr8_1:5349643-5349662 None:intergenic 45.0%
AATCTTACGGTCAAGACCAG+TGG - chr8_1:5349943-5349962 None:intergenic 45.0%
! CTCCTGGTGTTAATAGCAAC+TGG + chr8_1:5350027-5350046 Msa1174270:CDS 45.0%
! ACCCAGTTGCTATTAACACC+AGG - chr8_1:5350032-5350051 None:intergenic 45.0%
TCGGAACTCATGTGTCTACT+TGG - chr8_1:5350272-5350291 None:intergenic 45.0%
! AAGTACTTTTCGGCCTGATG+AGG - chr8_1:5350443-5350462 None:intergenic 45.0%
GCATAGTATTCGCCTGAACT+AGG - chr8_1:5350867-5350886 None:intergenic 45.0%
AGATGATTTCGGGGGATTTG+AGG + chr8_1:5350896-5350915 Msa1174270:CDS 45.0%
!!! CTTTTTGACATGGTGGTGGT+TGG - chr8_1:5351023-5351042 None:intergenic 45.0%
GGACGGAATCTGAATCCAAC+AGG - chr8_1:5349626-5349645 None:intergenic 50.0%
GTTGAACCTTGGGACTTACC+AGG + chr8_1:5349729-5349748 Msa1174270:intron 50.0%
GAAGGACACAGTGTGGATAC+TGG + chr8_1:5349913-5349932 Msa1174270:CDS 50.0%
TGTGGATACTGGAAAGCCAC+TGG + chr8_1:5349924-5349943 Msa1174270:CDS 50.0%
! TCATTCTCAACGACTCGGAC+TGG - chr8_1:5350678-5350697 None:intergenic 50.0%
GGACGGAATCTGAATCCAAC+AGG - chr8_1:5349626-5349645 None:intergenic 50.0%
GTTGAACCTTGGGACTTACC+AGG + chr8_1:5349729-5349748 Msa1174270:intron 50.0%
GAAGGACACAGTGTGGATAC+TGG + chr8_1:5349913-5349932 Msa1174270:CDS 50.0%
TGTGGATACTGGAAAGCCAC+TGG + chr8_1:5349924-5349943 Msa1174270:CDS 50.0%
! TCATTCTCAACGACTCGGAC+TGG - chr8_1:5350678-5350697 None:intergenic 50.0%
!! GACATGGTGGTGGTTGGTCT+TGG - chr8_1:5351017-5351036 None:intergenic 55.0%
!! GACATGGTGGTGGTTGGTCT+TGG - chr8_1:5351017-5351036 None:intergenic 55.0%
! CTCGGACTGGACAGGAAAGC+TGG - chr8_1:5350665-5350684 None:intergenic 60.0%
! CTCAACGACTCGGACTGGAC+AGG - chr8_1:5350673-5350692 None:intergenic 60.0%
! CTCGGACTGGACAGGAAAGC+TGG - chr8_1:5350665-5350684 None:intergenic 60.0%
! CTCAACGACTCGGACTGGAC+AGG - chr8_1:5350673-5350692 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 5349593 5351070 5349593 ID=Msa1174270;Name=Msa1174270
chr8_1 mRNA 5349593 5351070 5349593 ID=Msa1174270-mRNA-1;Parent=Msa1174270;Name=Msa1174270-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|0|0|1|1|1|6|0|302
chr8_1 exon 5349593 5349728 5349593 ID=Msa1174270-mRNA-1:exon:1775;Parent=Msa1174270-mRNA-1
chr8_1 exon 5349826 5350091 5349826 ID=Msa1174270-mRNA-1:exon:1776;Parent=Msa1174270-mRNA-1
chr8_1 exon 5350213 5350272 5350213 ID=Msa1174270-mRNA-1:exon:1777;Parent=Msa1174270-mRNA-1
chr8_1 exon 5350437 5350567 5350437 ID=Msa1174270-mRNA-1:exon:1778;Parent=Msa1174270-mRNA-1
chr8_1 exon 5350666 5350744 5350666 ID=Msa1174270-mRNA-1:exon:1779;Parent=Msa1174270-mRNA-1
chr8_1 exon 5350834 5351070 5350834 ID=Msa1174270-mRNA-1:exon:1780;Parent=Msa1174270-mRNA-1
chr8_1 CDS 5349593 5349728 5349593 ID=Msa1174270-mRNA-1:cds;Parent=Msa1174270-mRNA-1
chr8_1 CDS 5349826 5350091 5349826 ID=Msa1174270-mRNA-1:cds;Parent=Msa1174270-mRNA-1
chr8_1 CDS 5350213 5350272 5350213 ID=Msa1174270-mRNA-1:cds;Parent=Msa1174270-mRNA-1
chr8_1 CDS 5350437 5350567 5350437 ID=Msa1174270-mRNA-1:cds;Parent=Msa1174270-mRNA-1
chr8_1 CDS 5350666 5350744 5350666 ID=Msa1174270-mRNA-1:cds;Parent=Msa1174270-mRNA-1
chr8_1 CDS 5350834 5351070 5350834 ID=Msa1174270-mRNA-1:cds;Parent=Msa1174270-mRNA-1
Gene Sequence

>Msa1174270

ATGACAAACTCATTTCCTCATAACTATGTTCCTGTTGGATTCAGATTCCGTCCAACAGATGAAGAGCTTGTAAACCACTATCTCAAAAACAAGTTACTTGGTAATGATTCCACTGTCAACAATGTTATAGCTGCAACTTGTTCAATCATAAAATCCGATGATCCCGAATGGTTTTTCTTTAGTCCTCGTGATTACAAATACGCAAATAGTAAAAGGTTTAACAGAAGGACACAGTGTGGATACTGGAAAGCCACTGGTCTTGACCGTAAGATTAAGGTTAGAGGTACTGAAAATGTTATTGGGACAAAGAAAACACTTGTGTACTATAATGGTTGTGCTCCTGGTGTTAATAGCAACTGGGTGATTCATGAATATCATTCTACCACCTTTGATGATAACCAGAAAGCCGAGAAAAAAACTGAAGAGGAAACTGATAGAATGATATGTGATGAAGGGGGCCAAGAACCTCATCAGGCCGAAAAGTACTTTCCTTTTCCATACCAGTCTTCAATCTCTGAAAATGAGATGGAAGTATCTTTATCGAACTCAGGATTTTATGATTCCGAAAGTGGACACATCGATATGCAGAGTCCCTTTCCTGTCCAGTCCGAGTCGTTGAGAATGATTTACTATGGAAGCAGTGACACAAATGCTGAATTAGTCTCTATGCCCAATGGATACTTCAACGGCATTGTTGAACATCCTAGTTCAGGCGAATACTATGCATCATTTCGAGATGATTTCGGGGGATTTGAGGCATCATCTTGTGATTCAACTGCAGATAAACCTCTAGAGATGATCAATTGCAATGAAATTTCTAGTGTCAAAAAGAGCTGCAATAATCAGTATCATCCAAGACCAACCACCACCATGTCAAAAAGATCTGCTTCAAGACGATCTCAAACATAG
Protein sequence

>Msa1174270

MTNSFPHNYVPVGFRFRPTDEELVNHYLKNKLLGNDSTVNNVIAATCSIIKSDDPEWFFFSPRDYKYANSKRFNRRTQCGYWKATGLDRKIKVRGTENVIGTKKTLVYYNGCAPGVNSNWVIHEYHSTTFDDNQKAEKKTEEETDRMICDEGGQEPHQAEKYFPFPYQSSISENEMEVSLSNSGFYDSESGHIDMQSPFPVQSESLRMIYYGSSDTNAELVSMPNGYFNGIVEHPSSGEYYASFRDDFGGFEASSCDSTADKPLEMINCNEISSVKKSCNNQYHPRPTTTMSKRSASRRSQT*