Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1175020 | sp|Q9FKK2|AGL62_ARATH | 30.323 | 155 | 100 | 3 | 17 | 165 | 8 | 160 | 2.55e-17 | 80.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1175020 | G7L955 | 88.525 | 183 | 19 | 2 | 1 | 181 | 1 | 183 | 9.28e-113 | 328 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1175020 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1175020 | MtrunA17_Chr8g0345001 | 88.525 | 183 | 19 | 2 | 1 | 181 | 1 | 183 | 1.79e-116 | 328 |
| Msa1175020 | MtrunA17_Chr3g0093131 | 45.977 | 174 | 87 | 4 | 10 | 179 | 14 | 184 | 4.09e-39 | 132 |
| Msa1175020 | MtrunA17_Chr2g0302091 | 44.944 | 178 | 85 | 5 | 3 | 176 | 23 | 191 | 2.40e-35 | 122 |
| Msa1175020 | MtrunA17_Chr4g0018951 | 39.130 | 138 | 68 | 3 | 35 | 169 | 30 | 154 | 2.62e-23 | 90.9 |
| Msa1175020 | MtrunA17_Chr2g0333981 | 33.766 | 154 | 100 | 2 | 15 | 168 | 10 | 161 | 7.51e-21 | 84.7 |
| Msa1175020 | MtrunA17_Chr5g0422421 | 33.129 | 163 | 97 | 3 | 10 | 165 | 2 | 159 | 3.80e-18 | 79.0 |
| Msa1175020 | MtrunA17_Chr3g0089141 | 29.518 | 166 | 99 | 3 | 10 | 165 | 2 | 159 | 2.38e-17 | 76.6 |
| Msa1175020 | MtrunA17_Chr3g0099421 | 33.140 | 172 | 96 | 4 | 10 | 169 | 24 | 188 | 2.73e-17 | 77.0 |
| Msa1175020 | MtrunA17_Chr1g0208561 | 28.916 | 166 | 100 | 3 | 10 | 165 | 2 | 159 | 3.12e-17 | 76.3 |
| Msa1175020 | MtrunA17_Chr1g0149391 | 31.515 | 165 | 97 | 3 | 10 | 165 | 2 | 159 | 6.10e-17 | 75.5 |
| Msa1175020 | MtrunA17_Chr8g0379021 | 34.395 | 157 | 95 | 4 | 15 | 168 | 10 | 161 | 7.39e-17 | 74.3 |
| Msa1175020 | MtrunA17_Chr1g0206691 | 32.374 | 139 | 78 | 3 | 35 | 164 | 10 | 141 | 9.92e-17 | 74.7 |
| Msa1175020 | MtrunA17_Chr5g0422941 | 33.333 | 162 | 95 | 4 | 10 | 164 | 3 | 158 | 2.04e-16 | 74.3 |
| Msa1175020 | MtrunA17_Chr1g0206991 | 30.120 | 166 | 98 | 4 | 10 | 165 | 2 | 159 | 2.17e-16 | 73.2 |
| Msa1175020 | MtrunA17_Chr8g0355091 | 27.778 | 162 | 99 | 3 | 10 | 161 | 2 | 155 | 4.33e-16 | 73.6 |
| Msa1175020 | MtrunA17_Chr1g0206701 | 28.834 | 163 | 96 | 3 | 10 | 161 | 2 | 155 | 5.78e-16 | 73.2 |
| Msa1175020 | MtrunA17_Chr2g0292791 | 28.902 | 173 | 91 | 3 | 10 | 165 | 2 | 159 | 6.61e-16 | 72.8 |
| Msa1175020 | MtrunA17_Chr2g0317451 | 28.834 | 163 | 92 | 3 | 16 | 165 | 8 | 159 | 7.95e-16 | 72.8 |
| Msa1175020 | MtrunA17_Chr2g0317481 | 28.834 | 163 | 92 | 3 | 16 | 165 | 8 | 159 | 8.29e-16 | 72.8 |
| Msa1175020 | MtrunA17_Chr1g0206721 | 29.697 | 165 | 100 | 3 | 10 | 165 | 2 | 159 | 1.18e-15 | 72.0 |
| Msa1175020 | MtrunA17_Chr1g0208581 | 29.518 | 166 | 99 | 3 | 10 | 165 | 2 | 159 | 1.73e-15 | 71.6 |
| Msa1175020 | MtrunA17_Chr3g0119901 | 27.500 | 160 | 100 | 3 | 18 | 168 | 10 | 162 | 2.90e-15 | 70.5 |
| Msa1175020 | MtrunA17_Chr2g0300441 | 32.121 | 165 | 92 | 4 | 13 | 165 | 3 | 159 | 5.63e-15 | 70.5 |
| Msa1175020 | MtrunA17_Chr4g0071601 | 33.333 | 150 | 76 | 4 | 35 | 168 | 30 | 171 | 1.25e-14 | 68.6 |
| Msa1175020 | MtrunA17_Chr3g0085241 | 28.125 | 160 | 99 | 4 | 18 | 168 | 42 | 194 | 1.70e-14 | 69.3 |
| Msa1175020 | MtrunA17_Chr1g0188671 | 29.697 | 165 | 100 | 3 | 10 | 165 | 40 | 197 | 2.02e-14 | 69.3 |
| Msa1175020 | MtrunA17_Chr1g0180591 | 40.426 | 94 | 55 | 1 | 18 | 110 | 3 | 96 | 2.51e-14 | 67.4 |
| Msa1175020 | MtrunA17_Chr5g0419601 | 30.968 | 155 | 95 | 3 | 18 | 165 | 10 | 159 | 5.45e-14 | 67.8 |
| Msa1175020 | MtrunA17_Chr1g0187361 | 30.061 | 163 | 101 | 3 | 10 | 165 | 3 | 159 | 5.91e-14 | 67.8 |
| Msa1175020 | MtrunA17_Chr2g0325971 | 28.313 | 166 | 101 | 3 | 10 | 165 | 2 | 159 | 1.17e-13 | 66.6 |
| Msa1175020 | MtrunA17_Chr7g0238991 | 32.394 | 142 | 76 | 4 | 35 | 165 | 39 | 171 | 2.37e-13 | 66.2 |
| Msa1175020 | MtrunA17_Chr8g0376821 | 32.727 | 110 | 73 | 1 | 18 | 126 | 3 | 112 | 9.40e-13 | 63.5 |
| Msa1175020 | MtrunA17_Chr1g0169761 | 37.179 | 78 | 49 | 0 | 18 | 95 | 3 | 80 | 1.45e-12 | 62.8 |
| Msa1175020 | MtrunA17_Chr3g0099411 | 35.802 | 81 | 52 | 0 | 10 | 90 | 2 | 82 | 4.65e-12 | 62.0 |
| Msa1175020 | MtrunA17_Chr8g0357771 | 48.148 | 54 | 28 | 0 | 35 | 88 | 20 | 73 | 6.33e-12 | 61.2 |
| Msa1175020 | MtrunA17_Chr4g0074101 | 37.662 | 77 | 48 | 0 | 18 | 94 | 3 | 79 | 8.30e-12 | 61.2 |
| Msa1175020 | MtrunA17_Chr1g0175611 | 41.176 | 68 | 40 | 0 | 18 | 85 | 3 | 70 | 1.10e-11 | 60.5 |
| Msa1175020 | MtrunA17_Chr1g0175601 | 40.580 | 69 | 41 | 0 | 18 | 86 | 3 | 71 | 1.11e-11 | 60.5 |
| Msa1175020 | MtrunA17_Chr8g0356071 | 36.620 | 71 | 45 | 0 | 18 | 88 | 3 | 73 | 1.35e-11 | 60.1 |
| Msa1175020 | MtrunA17_Chr1g0187351 | 46.429 | 56 | 30 | 0 | 35 | 90 | 10 | 65 | 6.34e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1175020 | AT5G60440.1 | 30.323 | 155 | 100 | 3 | 17 | 165 | 8 | 160 | 2.60e-18 | 80.9 |
| Msa1175020 | AT2G24840.1 | 27.632 | 152 | 106 | 3 | 17 | 165 | 64 | 214 | 4.94e-14 | 68.6 |
| Msa1175020 | AT1G01530.1 | 31.138 | 167 | 99 | 5 | 11 | 169 | 2 | 160 | 3.69e-13 | 65.9 |
| Msa1175020 | AT3G66656.1 | 41.791 | 67 | 39 | 0 | 18 | 84 | 3 | 69 | 5.39e-13 | 64.3 |
| Msa1175020 | AT2G34440.1 | 29.530 | 149 | 95 | 2 | 18 | 161 | 3 | 146 | 5.54e-13 | 64.3 |
| Msa1175020 | AT3G04100.1 | 28.409 | 176 | 110 | 3 | 3 | 168 | 2 | 171 | 6.31e-13 | 64.7 |
| Msa1175020 | AT1G65360.1 | 44.118 | 68 | 37 | 1 | 17 | 84 | 8 | 74 | 1.98e-12 | 63.5 |
Find 21 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTCATTCTATTGATGATA+TGG | 0.250456 | 8_1:-6257160 | Msa1175020:CDS |
| TGAGCAATTTAAGACTTCTT+TGG | 0.270286 | 8_1:-6257124 | Msa1175020:CDS |
| TTAATGACGGTGTTGGGGTT+AGG | 0.312793 | 8_1:+6257363 | None:intergenic |
| GAAGAGAATAATTATTTAGA+AGG | 0.328216 | 8_1:-6257228 | Msa1175020:CDS |
| TTCTATTGATGATATGGCTT+TGG | 0.335359 | 8_1:-6257154 | Msa1175020:CDS |
| GAAGTTCATGTTATTCTTGT+TGG | 0.339097 | 8_1:-6257185 | Msa1175020:CDS |
| AAGTGTTTAATGACGGTGTT+GGG | 0.345862 | 8_1:+6257357 | None:intergenic |
| AATTTAGATGCATCCTTTGA+AGG | 0.376314 | 8_1:-6257087 | Msa1175020:CDS |
| TAATGACGGTGTTGGGGTTA+GGG | 0.379636 | 8_1:+6257364 | None:intergenic |
| CATTAAACACTTTCTCGATA+AGG | 0.404019 | 8_1:-6257346 | Msa1175020:CDS |
| AACCTTAAGGCTTGAAAATG+AGG | 0.417937 | 8_1:-6257271 | Msa1175020:CDS |
| GTTCATGTTATTCTTGTTGG+TGG | 0.430710 | 8_1:-6257182 | Msa1175020:CDS |
| AGGGCGTAGTTGAAACCTTA+AGG | 0.451953 | 8_1:-6257284 | Msa1175020:CDS |
| TTCATGTTATTCTTGTTGGT+GGG | 0.480773 | 8_1:-6257181 | Msa1175020:CDS |
| TTTCCGTCTGAAGAAGCCAT+TGG | 0.514844 | 8_1:+6257588 | None:intergenic |
| AAAGTGTTTAATGACGGTGT+TGG | 0.530631 | 8_1:+6257356 | None:intergenic |
| GATCCAATGGCTTCTTCAGA+CGG | 0.539894 | 8_1:-6257591 | None:intergenic |
| ACCTTCTCAAAACGCAAACT+AGG | 0.541621 | 8_1:-6257483 | Msa1175020:CDS |
| ATCGAGAAAGTGTTTAATGA+CGG | 0.587110 | 8_1:+6257350 | None:intergenic |
| AGTGTTTAATGACGGTGTTG+GGG | 0.595656 | 8_1:+6257358 | None:intergenic |
| AAAATGAGGCAATTGAAGAA+CGG | 0.623632 | 8_1:-6257257 | Msa1175020:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAGAGAATAATTATTTAGA+AGG | - | chr8_1:6257419-6257438 | Msa1175020:CDS | 20.0% |
| ! | TTCTGCTTTCTTGATTTTAG+TGG | + | chr8_1:6257123-6257142 | None:intergenic | 30.0% |
| !!! | CAAACGTAGAGTTTTTTGTT+TGG | + | chr8_1:6257257-6257276 | None:intergenic | 30.0% |
| AACAAAAAACTCTACGTTTG+TGG | - | chr8_1:6257257-6257276 | Msa1175020:CDS | 30.0% | |
| !! | ATCGAGAAAGTGTTTAATGA+CGG | + | chr8_1:6257300-6257319 | None:intergenic | 30.0% |
| CATTAAACACTTTCTCGATA+AGG | - | chr8_1:6257301-6257320 | Msa1175020:CDS | 30.0% | |
| AAAATGAGGCAATTGAAGAA+CGG | - | chr8_1:6257390-6257409 | Msa1175020:CDS | 30.0% | |
| GAAGTTCATGTTATTCTTGT+TGG | - | chr8_1:6257462-6257481 | Msa1175020:CDS | 30.0% | |
| TTCATGTTATTCTTGTTGGT+GGG | - | chr8_1:6257466-6257485 | Msa1175020:CDS | 30.0% | |
| ! | GGTTCATTCTATTGATGATA+TGG | - | chr8_1:6257487-6257506 | Msa1175020:CDS | 30.0% |
| ! | TTCTATTGATGATATGGCTT+TGG | - | chr8_1:6257493-6257512 | Msa1175020:CDS | 30.0% |
| ! | TGAGCAATTTAAGACTTCTT+TGG | - | chr8_1:6257523-6257542 | Msa1175020:CDS | 30.0% |
| AATTTAGATGCATCCTTTGA+AGG | - | chr8_1:6257560-6257579 | Msa1175020:CDS | 30.0% | |
| ! | ATGAATCTTTTTGCCTTCAA+AGG | + | chr8_1:6257576-6257595 | None:intergenic | 30.0% |
| AAGTGTTTAATGACGGTGTT+GGG | + | chr8_1:6257293-6257312 | None:intergenic | 35.0% | |
| ! | AAAGTGTTTAATGACGGTGT+TGG | + | chr8_1:6257294-6257313 | None:intergenic | 35.0% |
| AACCTTAAGGCTTGAAAATG+AGG | - | chr8_1:6257376-6257395 | Msa1175020:CDS | 35.0% | |
| GTTCATGTTATTCTTGTTGG+TGG | - | chr8_1:6257465-6257484 | Msa1175020:CDS | 35.0% | |
| !!! | TGCGTTTTGAGAAGGTATCA+CGG | + | chr8_1:6257160-6257179 | None:intergenic | 40.0% |
| ACCTTCTCAAAACGCAAACT+AGG | - | chr8_1:6257164-6257183 | Msa1175020:CDS | 40.0% | |
| !!! | TCCTAGTTTGCGTTTTGAGA+AGG | + | chr8_1:6257168-6257187 | None:intergenic | 40.0% |
| ! | AGTGTTTAATGACGGTGTTG+GGG | + | chr8_1:6257292-6257311 | None:intergenic | 40.0% |
| !!! | TTTTTTCAGCGTCGATGACT+GGG | + | chr8_1:6257331-6257350 | None:intergenic | 40.0% |
| TGAAAAAAGGACGCAAGATG+AGG | - | chr8_1:6257343-6257362 | Msa1175020:CDS | 40.0% | |
| GAAAAAAGGACGCAAGATGA+GGG | - | chr8_1:6257344-6257363 | Msa1175020:CDS | 40.0% | |
| !! | TGCCTCATTTTCAAGCCTTA+AGG | + | chr8_1:6257381-6257400 | None:intergenic | 40.0% |
| !!! | CGTTTTGAGAAGGTATCACG+GGG | + | chr8_1:6257158-6257177 | None:intergenic | 45.0% |
| !!! | GCGTTTTGAGAAGGTATCAC+GGG | + | chr8_1:6257159-6257178 | None:intergenic | 45.0% |
| ! | TAATGACGGTGTTGGGGTTA+GGG | + | chr8_1:6257286-6257305 | None:intergenic | 45.0% |
| ! | TTAATGACGGTGTTGGGGTT+AGG | + | chr8_1:6257287-6257306 | None:intergenic | 45.0% |
| !!! | CTTTTTTCAGCGTCGATGAC+TGG | + | chr8_1:6257332-6257351 | None:intergenic | 45.0% |
| CAGTCATCGACGCTGAAAAA+AGG | - | chr8_1:6257330-6257349 | Msa1175020:CDS | 45.0% | |
| AGGGCGTAGTTGAAACCTTA+AGG | - | chr8_1:6257363-6257382 | Msa1175020:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 6257062 | 6257607 | 6257062 | ID=Msa1175020;Name=Msa1175020 |
| chr8_1 | mRNA | 6257062 | 6257607 | 6257062 | ID=Msa1175020-mRNA-1;Parent=Msa1175020;Name=Msa1175020-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|181 |
| chr8_1 | exon | 6257062 | 6257607 | 6257062 | ID=Msa1175020-mRNA-1:exon:2164;Parent=Msa1175020-mRNA-1 |
| chr8_1 | CDS | 6257062 | 6257607 | 6257062 | ID=Msa1175020-mRNA-1:cds;Parent=Msa1175020-mRNA-1 |
| Gene Sequence |
| Protein sequence |
