AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1175510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1175510 MtrunA17_Chr8g0345701 63.253 166 17 3 1 127 1 161 6.43e-65 197
Msa1175510 MtrunA17_Chr8g0345691 35.448 268 75 9 7 180 12 275 5.76e-41 143
Msa1175510 MtrunA17_Chr8g0345671 51.095 137 39 3 7 115 11 147 1.88e-39 138
Msa1175510 MtrunA17_Chr8g0345711 44.928 138 47 3 6 114 11 148 1.17e-34 120
Msa1175510 MtrunA17_Chr8g0345731 43.750 144 48 5 7 117 24 167 4.41e-32 114
Msa1175510 MtrunA17_Chr8g0345721 37.821 156 41 3 9 108 37 192 5.37e-28 104
Msa1175510 MtrunA17_Chr8g0345741 42.424 132 40 4 18 117 2 129 2.16e-27 101
Msa1175510 MtrunA17_Chr3g0128151 43.511 131 43 5 6 108 21 148 8.69e-26 103
Msa1175510 MtrunA17_Chr5g0428991 41.538 130 45 4 7 108 25 151 1.22e-25 102
Msa1175510 MtrunA17_Chr3g0128111 40.152 132 48 5 10 113 45 173 3.38e-24 95.1
Msa1175510 MtrunA17_Chr3g0109821 35.526 152 64 5 5 128 4 149 2.25e-22 93.6
Msa1175510 MtrunA17_Chr2g0316971 39.062 128 51 3 7 108 23 149 4.38e-22 91.7
Msa1175510 MtrunA17_Chr2g0318101 37.143 140 59 4 7 120 15 151 1.02e-21 90.5
Msa1175510 MtrunA17_Chr4g0047841 32.323 198 98 7 7 172 15 208 1.49e-21 90.1
Msa1175510 MtrunA17_Chr2g0309891 37.405 131 51 4 6 108 5 132 1.57e-21 89.7
Msa1175510 MtrunA17_Chr6g0453451 39.844 128 48 4 7 108 20 144 1.67e-21 89.4
Msa1175510 MtrunA17_Chr6g0484441 38.281 128 50 4 7 108 5 129 2.62e-21 89.0
Msa1175510 MtrunA17_Chr1g0197361 36.296 135 56 3 2 108 3 135 2.78e-21 90.5
Msa1175510 MtrunA17_Chr3g0124761 33.987 153 71 4 1 127 1 149 2.92e-21 88.2
Msa1175510 MtrunA17_Chr5g0433781 36.735 147 58 6 5 122 4 144 2.95e-21 90.5
Msa1175510 MtrunA17_Chr1g0147421 36.842 133 58 2 2 108 22 154 3.27e-21 89.4
Msa1175510 MtrunA17_Chr3g0144271 36.429 140 60 4 7 117 23 162 5.55e-21 89.0
Msa1175510 MtrunA17_Chr4g0060931 41.406 128 46 5 7 108 17 141 5.95e-21 88.6
Msa1175510 MtrunA17_Chr6g0481091 37.063 143 56 5 3 117 2 138 9.23e-21 86.3
Msa1175510 MtrunA17_Chr3g0130101 37.671 146 56 6 2 116 3 144 1.01e-20 87.8
Msa1175510 MtrunA17_Chr1g0183741 34.848 132 59 3 2 107 5 135 1.20e-20 86.7
Msa1175510 MtrunA17_Chr6g0461661 31.000 200 109 5 7 179 18 215 1.67e-20 87.4
Msa1175510 MtrunA17_Chr8g0385991 34.109 129 58 4 5 108 12 138 1.75e-20 85.1
Msa1175510 MtrunA17_Chr7g0214071 37.600 125 54 3 7 108 15 138 1.88e-20 85.5
Msa1175510 MtrunA17_Chr5g0400461 34.532 139 57 4 5 110 4 141 1.98e-20 86.7
Msa1175510 MtrunA17_Chr7g0261411 34.586 133 60 3 2 108 5 136 3.10e-20 85.5
Msa1175510 MtrunA17_Chr3g0139331 37.143 140 57 4 6 115 10 148 3.59e-20 86.3
Msa1175510 MtrunA17_Chr3g0091921 40.625 128 47 5 7 108 16 140 4.59e-20 85.5
Msa1175510 MtrunA17_Chr7g0253011 38.462 130 51 4 7 108 17 145 1.86e-19 84.7
Msa1175510 MtrunA17_Chr3g0130731 32.680 153 73 4 1 127 1 149 6.08e-19 82.0
Msa1175510 MtrunA17_Chr4g0054791 33.835 133 58 4 5 108 4 135 8.76e-19 82.0
Msa1175510 MtrunA17_Chr1g0201021 35.417 144 61 5 2 117 15 154 1.01e-18 83.2
Msa1175510 MtrunA17_Chr3g0092671 35.433 127 52 3 10 108 8 132 1.02e-18 83.2
Msa1175510 MtrunA17_Chr8g0364791 33.071 127 54 4 10 108 9 132 1.28e-18 82.4
Msa1175510 MtrunA17_Chr4g0042561 35.294 136 57 5 1 108 1 133 1.98e-18 80.5
Msa1175510 MtrunA17_Chr4g0026141 33.333 150 68 5 7 125 5 153 3.07e-18 79.7
Msa1175510 MtrunA17_Chr7g0217721 40.800 125 45 5 10 108 22 143 3.20e-18 80.9
Msa1175510 MtrunA17_Chr5g0398971 32.867 143 65 4 1 114 1 141 3.97e-18 80.5
Msa1175510 MtrunA17_Chr2g0312321 36.719 128 50 4 7 108 11 133 4.28e-18 80.1
Msa1175510 MtrunA17_Chr8g0384721 34.328 134 58 4 1 108 1 130 4.35e-18 79.7
Msa1175510 MtrunA17_Chr4g0056541 31.724 145 67 4 1 116 1 142 5.38e-18 80.5
Msa1175510 MtrunA17_Chr8g0384201 33.858 127 53 4 10 108 9 132 6.06e-18 80.9
Msa1175510 MtrunA17_Chr4g0018391 32.353 136 60 4 1 107 1 133 7.59e-18 79.7
Msa1175510 MtrunA17_Chr8g0359921 35.659 129 54 4 6 108 13 138 2.29e-17 78.2
Msa1175510 MtrunA17_Chr7g0263931 33.594 128 58 3 7 108 11 137 2.72e-17 78.2
Msa1175510 MtrunA17_Chr2g0326361 27.604 192 107 5 2 164 9 197 2.77e-17 78.2
Msa1175510 MtrunA17_Chr4g0076731 31.844 179 78 7 7 159 55 215 3.56e-17 78.6
Msa1175510 MtrunA17_Chr7g0217711 39.200 125 47 5 10 108 22 143 3.64e-17 75.1
Msa1175510 MtrunA17_Chr1g0200561 31.469 143 66 4 1 114 13 152 4.27e-17 77.8
Msa1175510 MtrunA17_Chr7g0252971 34.307 137 56 4 5 108 6 141 5.07e-17 77.4
Msa1175510 MtrunA17_Chr8g0361731 28.191 188 99 8 10 168 17 197 9.94e-17 77.0
Msa1175510 MtrunA17_Chr2g0318081 34.884 129 55 4 6 108 15 140 1.58e-16 76.3
Msa1175510 MtrunA17_Chr1g0197351 35.878 131 53 4 5 107 40 167 1.78e-16 76.6
Msa1175510 MtrunA17_Chr5g0405211 30.075 133 61 4 5 108 6 135 2.79e-16 75.5
Msa1175510 MtrunA17_Chr8g0372121 30.075 133 61 4 5 108 4 133 4.06e-16 74.7
Msa1175510 MtrunA17_Chr2g0311661 33.071 127 55 4 10 107 24 149 7.78e-16 73.6
Msa1175510 MtrunA17_Chr2g0311651 32.812 128 56 4 10 108 25 151 1.16e-15 73.6
Msa1175510 MtrunA17_Chr7g0266701 31.373 153 70 5 7 125 4 155 1.52e-15 72.4
Msa1175510 MtrunA17_Chr5g0417791 32.812 128 57 4 7 108 3 127 2.67e-15 71.6
Msa1175510 MtrunA17_Chr5g0417001 31.200 125 59 3 10 108 17 140 3.64e-15 72.4
Msa1175510 MtrunA17_Chr6g0453131 34.375 128 55 5 7 108 16 140 4.29e-15 72.4
Msa1175510 MtrunA17_Chr4g0038751 34.127 126 55 4 10 108 18 142 5.24e-15 71.6
Msa1175510 MtrunA17_Chr3g0131911 32.061 131 60 5 5 108 12 140 1.93e-14 69.3
Msa1175510 MtrunA17_Chr4g0017921 31.852 135 60 4 3 108 4 135 5.68e-14 68.9
Msa1175510 MtrunA17_Chr2g0321741 38.318 107 36 5 28 107 13 116 6.13e-14 68.9
Msa1175510 MtrunA17_Chr2g0309331 31.159 138 64 4 6 114 9 144 6.95e-14 68.6
Msa1175510 MtrunA17_Chr5g0442771 32.540 126 57 4 10 108 21 145 7.88e-14 68.2
Msa1175510 MtrunA17_Chr8g0346001 29.323 133 62 4 5 108 13 142 2.18e-13 67.4
Msa1175510 MtrunA17_Chr8g0384351 35.766 137 58 6 2 123 5 126 2.28e-13 65.5
Msa1175510 MtrunA17_Chr4g0050701 32.000 125 56 5 7 108 11 129 2.65e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1175510 AT4G35580.3 44.853 136 44 4 6 113 8 140 4.27e-31 118
Msa1175510 AT4G35580.2 44.853 136 44 4 6 113 8 140 4.35e-31 118
Msa1175510 AT4G35580.1 44.853 136 44 4 6 113 8 140 4.96e-31 118
Msa1175510 AT1G33060.2 42.424 132 45 4 6 109 23 151 1.14e-29 114
Msa1175510 AT1G33060.1 42.424 132 45 4 6 109 23 151 1.23e-29 114
Msa1175510 AT3G49530.2 38.971 136 53 4 6 113 12 145 6.41e-26 103
Msa1175510 AT5G24590.2 40.146 137 52 4 5 113 11 145 6.88e-26 103
Msa1175510 AT3G49530.1 38.971 136 53 4 6 113 12 145 7.24e-26 103
Msa1175510 AT4G01550.1 42.636 129 45 3 9 108 6 134 6.64e-24 97.8
Msa1175510 AT4G17980.1 35.338 133 57 3 4 108 3 134 1.30e-23 94.4
Msa1175510 AT4G17980.2 35.338 133 57 3 4 108 3 134 2.51e-23 94.4
Msa1175510 AT5G53950.1 39.695 131 52 3 4 108 14 143 2.73e-23 95.5
Msa1175510 AT3G10490.1 38.806 134 51 4 3 108 23 153 4.74e-23 92.4
Msa1175510 AT5G22290.1 39.552 134 50 5 1 107 16 145 5.14e-23 94.4
Msa1175510 AT4G01520.1 38.931 131 50 4 8 108 5 135 8.15e-23 93.2
Msa1175510 AT3G10480.1 39.552 134 50 4 3 108 23 153 8.81e-23 94.7
Msa1175510 AT3G10480.2 39.552 134 50 4 3 108 23 153 1.08e-22 94.4
Msa1175510 AT3G10490.2 38.806 134 51 4 3 108 23 153 1.15e-22 94.4
Msa1175510 AT4G01540.3 40.769 130 47 4 9 108 6 135 1.32e-22 94.0
Msa1175510 AT3G15170.1 37.584 149 64 4 1 121 14 161 1.46e-22 92.4
Msa1175510 AT4G01540.1 40.769 130 47 4 9 108 6 135 1.48e-22 94.4
Msa1175510 AT2G27300.1 31.390 223 105 9 10 187 17 236 1.64e-22 92.8
Msa1175510 AT4G01540.2 40.769 130 47 4 9 108 6 135 1.80e-22 93.6
Msa1175510 AT4G01540.4 40.769 130 47 4 9 108 6 135 2.48e-22 92.8
Msa1175510 AT1G02230.1 37.984 129 53 3 8 109 4 132 6.61e-22 91.7
Msa1175510 AT5G61430.1 35.028 177 82 7 2 148 11 184 7.69e-22 90.9
Msa1175510 AT3G17730.1 37.762 143 55 5 6 120 5 141 8.47e-22 89.4
Msa1175510 AT5G17260.1 35.526 152 64 5 5 128 4 149 1.22e-21 91.7
Msa1175510 AT3G18400.1 38.281 128 50 4 7 108 5 129 1.51e-21 89.7
Msa1175510 AT3G04060.1 38.281 128 51 4 7 108 20 145 1.59e-21 90.1
Msa1175510 AT5G46590.2 35.338 133 56 4 4 108 3 133 1.80e-21 89.4
Msa1175510 AT5G18270.2 39.062 128 50 4 7 108 21 146 1.83e-21 90.1
Msa1175510 AT5G46590.1 35.338 133 56 4 4 108 3 133 1.97e-21 89.4
Msa1175510 AT5G18270.1 39.062 128 50 4 7 108 21 146 2.31e-21 89.7
Msa1175510 AT5G04410.1 37.931 145 59 4 5 121 7 148 2.59e-21 90.9
Msa1175510 AT1G01720.1 37.313 134 54 4 1 108 1 130 7.61e-21 87.8
Msa1175510 AT3G44290.2 39.394 132 49 5 3 107 11 138 8.70e-21 86.3
Msa1175510 AT5G39610.1 40.602 133 50 5 2 108 15 144 1.33e-20 87.0
Msa1175510 AT1G65910.1 38.462 130 49 4 6 107 5 131 1.41e-20 89.0
Msa1175510 AT3G44290.1 39.394 132 49 5 3 107 11 138 3.49e-20 86.7
Msa1175510 AT2G24430.2 37.209 129 54 3 6 108 15 142 4.39e-20 85.9
Msa1175510 AT2G24430.1 37.209 129 54 3 6 108 15 142 4.39e-20 85.9
Msa1175510 AT1G02250.1 37.121 132 56 3 8 112 4 135 7.56e-20 85.9
Msa1175510 AT5G07680.2 39.062 128 49 5 7 108 3 127 8.78e-20 85.1
Msa1175510 AT5G07680.1 39.062 128 49 5 7 108 17 141 1.01e-19 85.1
Msa1175510 AT3G10500.1 39.394 132 49 5 5 108 7 135 1.30e-19 85.9
Msa1175510 AT5G13180.1 35.200 125 57 3 7 108 14 137 1.39e-19 83.6
Msa1175510 AT3G29035.1 40.625 128 47 5 7 108 24 148 1.54e-19 84.3
Msa1175510 AT5G08790.1 33.582 134 59 4 1 108 1 130 3.36e-19 83.2
Msa1175510 AT3G03200.1 34.437 151 65 5 6 128 5 149 4.10e-19 84.3
Msa1175510 AT5G39820.1 35.461 141 63 4 10 123 23 162 4.99e-19 83.2
Msa1175510 AT1G77450.1 34.694 147 66 4 7 127 10 152 5.44e-19 82.0
Msa1175510 AT2G33480.1 36.800 125 53 4 7 108 15 136 6.15e-19 82.0
Msa1175510 AT2G33480.2 36.800 125 53 4 7 108 15 136 6.22e-19 82.0
Msa1175510 AT1G56010.2 35.417 144 62 4 7 121 19 160 6.59e-19 82.8
Msa1175510 AT1G76420.1 36.434 129 53 4 6 107 21 147 1.08e-18 82.4
Msa1175510 AT3G12910.1 32.192 146 71 5 2 120 17 161 1.22e-18 82.0
Msa1175510 AT3G04420.2 36.641 131 52 4 8 109 4 132 4.52e-18 80.1
Msa1175510 AT3G04070.2 36.842 133 54 4 5 108 8 139 6.72e-18 80.1
Msa1175510 AT3G04420.1 36.641 131 52 4 8 109 4 132 7.30e-18 80.1
Msa1175510 AT2G17040.1 35.971 139 57 6 10 121 9 142 8.49e-18 79.3
Msa1175510 AT3G04070.1 36.842 133 54 4 5 108 8 139 9.13e-18 80.1
Msa1175510 AT1G71930.2 33.333 132 56 4 5 107 7 135 1.22e-17 79.3
Msa1175510 AT1G71930.1 33.333 132 56 4 5 107 7 135 1.22e-17 79.3
Msa1175510 AT1G52890.1 37.879 132 53 5 3 108 10 138 1.25e-17 79.3
Msa1175510 AT5G63790.1 32.090 134 61 4 1 108 44 173 1.30e-17 79.3
Msa1175510 AT1G69490.1 32.061 131 60 4 3 107 5 132 1.33e-17 78.6
Msa1175510 AT5G63790.2 32.090 134 61 4 1 108 54 183 1.54e-17 79.0
Msa1175510 AT1G54330.1 38.168 131 50 4 6 108 5 132 1.61e-17 79.0
Msa1175510 AT3G15500.1 35.659 129 54 4 6 108 13 138 1.91e-17 79.0
Msa1175510 AT1G34190.1 35.000 140 60 4 10 121 19 155 2.00e-17 79.7
Msa1175510 AT4G27410.2 34.884 129 55 4 6 108 13 138 2.07e-17 78.6
Msa1175510 AT1G26870.1 34.677 124 54 3 10 107 19 141 2.72e-17 79.0
Msa1175510 AT4G36160.1 31.387 137 62 4 1 108 4 137 3.33e-17 78.6
Msa1175510 AT4G36160.3 31.387 137 62 4 1 108 4 137 3.33e-17 78.6
Msa1175510 AT4G36160.2 31.387 137 62 4 1 108 8 141 3.61e-17 78.6
Msa1175510 AT5G64530.1 38.983 118 51 5 7 107 3 116 4.45e-17 75.5
Msa1175510 AT5G64530.2 37.931 116 55 4 7 107 3 116 6.77e-17 74.3
Msa1175510 AT1G12260.2 31.387 137 62 4 1 108 1 134 7.39e-17 77.4
Msa1175510 AT1G12260.1 31.618 136 61 4 1 107 1 133 9.74e-17 77.4
Msa1175510 AT2G02450.1 30.818 159 81 5 7 139 52 207 1.03e-16 77.4
Msa1175510 AT1G61110.1 37.209 129 49 5 7 107 16 140 1.06e-16 76.6
Msa1175510 AT1G62700.1 32.168 143 65 4 1 114 1 140 1.20e-16 77.0
Msa1175510 AT1G62700.2 32.168 143 65 4 1 114 1 140 1.20e-16 77.0
Msa1175510 AT1G34180.1 38.095 126 47 4 10 107 19 141 1.28e-16 77.4
Msa1175510 AT2G18060.3 31.343 134 60 4 4 108 6 136 1.39e-16 76.6
Msa1175510 AT2G18060.1 31.343 134 60 4 4 108 6 136 1.39e-16 76.6
Msa1175510 AT2G02450.2 30.818 159 81 5 7 139 52 207 1.48e-16 77.0
Msa1175510 AT1G32510.1 34.328 134 54 4 7 107 6 138 1.68e-16 75.9
Msa1175510 AT5G66300.1 30.370 135 62 4 4 109 9 140 1.69e-16 75.9
Msa1175510 AT1G34180.3 38.095 126 47 4 10 107 19 141 1.85e-16 76.6
Msa1175510 AT5G09330.5 34.848 132 55 4 5 108 4 132 2.89e-16 76.3
Msa1175510 AT5G09330.2 34.848 132 55 4 5 108 4 132 2.89e-16 76.3
Msa1175510 AT5G09330.4 34.848 132 55 4 5 108 4 132 2.89e-16 76.3
Msa1175510 AT5G09330.3 34.848 132 55 4 5 108 4 132 2.89e-16 76.3
Msa1175510 AT5G09330.1 34.848 132 55 4 5 108 4 132 2.89e-16 76.3
Msa1175510 AT3G61910.1 30.827 133 60 4 5 108 9 138 5.92e-16 74.7
Msa1175510 AT3G15510.1 33.871 124 55 3 11 108 21 143 1.40e-15 73.9
Msa1175510 AT5G62380.2 32.353 136 60 4 1 107 1 133 1.56e-15 73.6
Msa1175510 AT5G62380.1 32.353 136 60 4 1 107 1 133 1.56e-15 73.6
Msa1175510 AT4G28530.1 29.605 152 56 4 2 107 5 151 1.67e-15 73.6
Msa1175510 AT3G04430.1 33.005 203 85 9 11 171 2 195 2.18e-15 71.2
Msa1175510 AT3G12977.1 33.077 130 56 5 5 107 16 141 3.94e-15 72.0
Msa1175510 AT1G02220.1 34.507 142 60 5 1 116 1 135 5.53e-15 72.4
Msa1175510 AT5G14000.2 34.307 137 60 5 7 116 15 148 7.22e-15 68.9
Msa1175510 AT1G01010.1 34.586 133 54 4 9 108 5 137 8.73e-15 72.0
Msa1175510 AT5G14000.1 34.307 137 60 5 7 116 15 148 2.83e-14 68.6
Msa1175510 AT5G22380.1 31.081 148 70 4 9 125 7 153 2.91e-14 68.9
Msa1175510 AT2G43000.1 30.952 126 56 5 10 108 21 142 3.64e-14 69.3
Msa1175510 AT1G33280.1 29.861 144 69 4 3 117 4 144 4.08e-14 69.3
Msa1175510 AT1G34180.2 34.783 138 47 5 10 107 19 153 1.45e-13 68.6
Msa1175510 AT2G46770.1 30.137 146 66 6 5 121 14 152 1.58e-13 68.2
Msa1175510 AT4G10350.1 31.343 134 60 4 3 107 5 135 1.72e-13 67.8
Msa1175510 AT1G34180.4 34.783 138 47 5 10 107 19 153 1.92e-13 68.2
Msa1175510 AT3G44350.1 28.571 133 64 3 7 109 5 136 3.55e-13 64.7
Msa1175510 AT5G64060.1 31.818 132 59 5 5 108 4 132 9.30e-13 65.9
Msa1175510 AT1G32770.1 28.571 133 63 4 5 108 14 143 1.35e-11 62.4

Find 30 sgRNAs with CRISPR-Local

Find 328 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCCCCTGAATTGAATA+TGG 0.261808 8_1:+6870551 Msa1175510:CDS
TTCGTTCGTTGATTCAATTT+TGG 0.274129 8_1:+6870710 Msa1175510:CDS
AAATCTTCCATATTCAATTC+AGG 0.275499 8_1:-6870558 None:intergenic
TACCAAGCAACTTGTGATTT+AGG 0.276773 8_1:-6866000 None:intergenic
TGAAATCGGAAGATGGTTAT+TGG 0.315021 8_1:+6866234 Msa1175510:CDS
CTATGCACCATGGGATTTAC+CGG 0.320849 8_1:+6866069 Msa1175510:CDS
AAAGGTACCAACATTGTTAT+AGG 0.339234 8_1:+6866290 Msa1175510:CDS
ATCAACATGAAAAGACCAAC+TGG 0.400465 8_1:+6866342 Msa1175510:CDS
ATGCTAAATTCCTCTTCCTT+CGG 0.407304 8_1:-6870594 None:intergenic
GGTCTGAATCTATAACCTAT+AGG 0.416888 8_1:-6865950 None:intergenic
AGCATTGTCCAAGGTGAAAT+CGG 0.439567 8_1:+6866220 Msa1175510:intron
CTCACAAGTTCTTGATCTGT+TGG 0.457501 8_1:-6865971 None:intergenic
AATCTTCCATATTCAATTCA+GGG 0.466548 8_1:-6870557 None:intergenic
TACCTAAATCACAAGTTGCT+TGG 0.491048 8_1:+6865998 Msa1175510:CDS
ACCTATAGGCATTGAAGTTG+AGG 0.493740 8_1:-6865936 None:intergenic
GATCGTGAAATCAAGACAAA+AGG 0.495582 8_1:+6866272 Msa1175510:CDS
ACCTCAACTTCAATGCCTAT+AGG 0.499800 8_1:+6865935 Msa1175510:CDS
TCTACCTGAAGCTCTTGAAA+AGG 0.505581 8_1:+6870671 Msa1175510:CDS
AGCATAAATTCAATGCTACA+AGG 0.545215 8_1:+6870612 Msa1175510:CDS
ACCATCTTCCGATTTCACCT+TGG 0.548691 8_1:-6866228 None:intergenic
TCCAAGGTGAAATCGGAAGA+TGG 0.551239 8_1:+6866227 Msa1175510:CDS
CGTGTTATTAGCATTGTCCA+AGG 0.553292 8_1:+6866211 Msa1175510:intron
GATGGTTATTGGAAGATCAC+TGG 0.556928 8_1:+6866245 Msa1175510:CDS
ATCTTACCCGGTAAATCCCA+TGG 0.558835 8_1:-6866076 None:intergenic
CTTGAGAACGAAAACAGCAT+TGG 0.575656 8_1:+6870642 Msa1175510:CDS
ATCTTCCATATTCAATTCAG+GGG 0.593727 8_1:-6870556 None:intergenic
TAGTTTAAAAGTTGAACATG+AGG 0.608519 8_1:-6866049 None:intergenic
ATACTGATGCATAACCCAGT+TGG 0.622932 8_1:-6866357 None:intergenic
TATGCACCATGGGATTTACC+GGG 0.653252 8_1:+6866070 Msa1175510:CDS
TCAACATGAAAAGACCAACT+GGG 0.701563 8_1:+6866343 Msa1175510:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAAAATTATTTATATT+TGG + chr8_1:6870213-6870232 Msa1175510:intron 0.0%
!!! TATTTTTATTTTTTGCATTG+AGG - chr8_1:6866115-6866134 None:intergenic 15.0%
!!! TGCTTTATTTTATTTTGAAA+AGG + chr8_1:6866412-6866431 Msa1175510:intron 15.0%
!! TATAATGTTATTATATACTC+TGG + chr8_1:6866447-6866466 Msa1175510:intron 15.0%
!! ATAATGTTATTATATACTCT+GGG + chr8_1:6866448-6866467 Msa1175510:intron 15.0%
!!! ATTGATTTTTCAATCTAAAA+AGG + chr8_1:6867642-6867661 Msa1175510:intron 15.0%
!!! TTGATTTTTCAATCTAAAAA+GGG + chr8_1:6867643-6867662 Msa1175510:intron 15.0%
!! ATTGAAGATAAAAAGATAAA+TGG - chr8_1:6867689-6867708 None:intergenic 15.0%
!!! AAAACTTTGAAAAATTACAT+AGG - chr8_1:6867713-6867732 None:intergenic 15.0%
!!! TAATTTTTCAAAGTTTTGTT+GGG + chr8_1:6867716-6867735 Msa1175510:intron 15.0%
!! AAAATTTGCAATACATTTAT+AGG + chr8_1:6867814-6867833 Msa1175510:intron 15.0%
!! AAATTTGCAATACATTTATA+GGG + chr8_1:6867815-6867834 Msa1175510:intron 15.0%
!! TATATAAATCCCTATAAATT+GGG - chr8_1:6867889-6867908 None:intergenic 15.0%
!! TTATATAAATCCCTATAAAT+TGG - chr8_1:6867890-6867909 None:intergenic 15.0%
!! GATTTATATAAGAAATTCAA+GGG + chr8_1:6867899-6867918 Msa1175510:intron 15.0%
!! ATTTATATAAGAAATTCAAG+GGG + chr8_1:6867900-6867919 Msa1175510:intron 15.0%
!! ATAAGAAATTGCAAAAAATT+TGG + chr8_1:6867929-6867948 Msa1175510:intron 15.0%
!! TAAGAAATTGCAAAAAATTT+GGG + chr8_1:6867930-6867949 Msa1175510:intron 15.0%
!! TGAAAAAAAAAAAAAAGAAG+AGG + chr8_1:6868094-6868113 Msa1175510:intron 15.0%
!!! AATGATGATTTGAATTTTTT+TGG + chr8_1:6868522-6868541 Msa1175510:intron 15.0%
!! ATATGTTTGATTATTTGATT+CGG + chr8_1:6868753-6868772 Msa1175510:intron 15.0%
!! TTGATTTCTATTATTCATAT+TGG + chr8_1:6869161-6869180 Msa1175510:intron 15.0%
!!! TGACATATTTAATTGTATTT+TGG + chr8_1:6869860-6869879 Msa1175510:intron 15.0%
!! AGAATTGAATGTAAAAAAAA+TGG - chr8_1:6870114-6870133 None:intergenic 15.0%
!!! ACAAAGATATTACTTTTTAA+AGG + chr8_1:6870405-6870424 Msa1175510:intron 15.0%
!!! CAAAGATATTACTTTTTAAA+GGG + chr8_1:6870406-6870425 Msa1175510:intron 15.0%
!! TTTAATGGGGTTATATTAAA+TGG + chr8_1:6866157-6866176 Msa1175510:intron 20.0%
!!! AAGCAATTTTAATCAAGTAT+TGG - chr8_1:6866498-6866517 None:intergenic 20.0%
!! ATCTAAAGAAAAATCAAACA+CGG - chr8_1:6866557-6866576 None:intergenic 20.0%
!! ATTGACTAAAAATTCATCAA+AGG - chr8_1:6866639-6866658 None:intergenic 20.0%
!!! TGACTTTTTATTTATGACTA+AGG + chr8_1:6866690-6866709 Msa1175510:intron 20.0%
!!! ATTGAAACTTTGAAACTTAA+AGG - chr8_1:6866793-6866812 None:intergenic 20.0%
!! AAAACTTAAAAGACTAACTT+GGG - chr8_1:6866841-6866860 None:intergenic 20.0%
!! TAAAACTTAAAAGACTAACT+TGG - chr8_1:6866842-6866861 None:intergenic 20.0%
!! GAAATAGTTTATCAACTAAA+AGG - chr8_1:6867246-6867265 None:intergenic 20.0%
!! GAAATAGTTTATCAACTAAA+AGG + chr8_1:6867246-6867265 Msa1175510:intron 20.0%
!!! AAGTTTCTAAAGTTTCAAAT+AGG - chr8_1:6867273-6867292 None:intergenic 20.0%
!!! TTGAAACTTTAGAAACTTAA+AGG + chr8_1:6867275-6867294 Msa1175510:intron 20.0%
!!! TTGAAACTTTTGAAACTTAA+AGG + chr8_1:6867335-6867354 Msa1175510:intron 20.0%
!! TGTATATTGAATTAAATCCA+CGG - chr8_1:6867404-6867423 None:intergenic 20.0%
!!! GTAATTTTTCAAAGTTTTGT+TGG + chr8_1:6867715-6867734 Msa1175510:intron 20.0%
!! AATTTGCAATACATTTATAG+GGG + chr8_1:6867816-6867835 Msa1175510:intron 20.0%
!! GGATTTATATAAGAAATTCA+AGG + chr8_1:6867898-6867917 Msa1175510:intron 20.0%
!!! CATTTTTAGTTTCTTGTTTA+CGG + chr8_1:6868162-6868181 Msa1175510:intron 20.0%
!!! ATCAATTTGTTGTTTTGTTA+TGG + chr8_1:6868439-6868458 Msa1175510:intron 20.0%
!!! TTTTTTTTAGCTCTCTTTAT+TGG + chr8_1:6868601-6868620 Msa1175510:intron 20.0%
!! GAATAAACCATCAATTTAAT+AGG - chr8_1:6869583-6869602 None:intergenic 20.0%
!! ATGTAAAAATAAGATAAAGC+TGG + chr8_1:6870029-6870048 Msa1175510:intron 20.0%
!!! ATCTCACTTTTAAACAAAAA+CGG - chr8_1:6870192-6870211 None:intergenic 20.0%
!!! TGATTTTATAATAACCACAA+AGG + chr8_1:6870324-6870343 Msa1175510:intron 20.0%
!!! GATTTTATAATAACCACAAA+GGG + chr8_1:6870325-6870344 Msa1175510:intron 20.0%
!! TGCATAACATAAATTCAAAA+GGG - chr8_1:6870487-6870506 None:intergenic 20.0%
!! ATGCATAACATAAATTCAAA+AGG - chr8_1:6870488-6870507 None:intergenic 20.0%
! TAGTTTAAAAGTTGAACATG+AGG - chr8_1:6866052-6866071 None:intergenic 25.0%
!!! TTTTATTTTTTGCATTGAGG+AGG - chr8_1:6866112-6866131 None:intergenic 25.0%
!! TACATGTTTTCCTACTTTAA+TGG + chr8_1:6866142-6866161 Msa1175510:intron 25.0%
!! ACATGTTTTCCTACTTTAAT+GGG + chr8_1:6866143-6866162 Msa1175510:intron 25.0%
! TAATATAACCCCATTAAAGT+AGG - chr8_1:6866155-6866174 None:intergenic 25.0%
!! TTTTATGCCTATAACAATGT+TGG - chr8_1:6866300-6866319 None:intergenic 25.0%
!!! AAAAAGACTCTTGTTTTCTA+TGG + chr8_1:6866317-6866336 Msa1175510:CDS 25.0%
!! TTCAAACTTTTCTGATAACA+AGG + chr8_1:6866382-6866401 Msa1175510:CDS 25.0%
! TGATTAAAATTGCTTCTCTT+TGG + chr8_1:6866503-6866522 Msa1175510:intron 25.0%
! CTTTCTAATGTCAAAATGAT+CGG - chr8_1:6866589-6866608 None:intergenic 25.0%
! CATGCAATTTGAGAAAAATT+AGG - chr8_1:6866672-6866691 None:intergenic 25.0%
! AACTGTTTGCAAGAAATTTA+AGG + chr8_1:6866734-6866753 Msa1175510:intron 25.0%
! ACTGTTTGCAAGAAATTTAA+GGG + chr8_1:6866735-6866754 Msa1175510:intron 25.0%
! TAATTATTAACTGACTAGCA+AGG + chr8_1:6867215-6867234 Msa1175510:intron 25.0%
! AATTATTAACTGACTAGCAA+GGG + chr8_1:6867216-6867235 Msa1175510:intron 25.0%
! GCAAATTCTATCAAACTAAA+AGG + chr8_1:6867306-6867325 Msa1175510:intron 25.0%
! GAAAATTCTGTCAAACTAAA+AGG + chr8_1:6867366-6867385 Msa1175510:intron 25.0%
! TAATTCAATATACAAGTGAG+TGG + chr8_1:6867410-6867429 Msa1175510:intron 25.0%
! AATTCAATATACAAGTGAGT+GGG + chr8_1:6867411-6867430 Msa1175510:intron 25.0%
! AAAGAAATTGCCCAATTTAT+AGG + chr8_1:6867876-6867895 Msa1175510:intron 25.0%
! AAGAAATTGCCCAATTTATA+GGG + chr8_1:6867877-6867896 Msa1175510:intron 25.0%
! TGGTGTTTCTTTGAATAATT+TGG + chr8_1:6868459-6868478 Msa1175510:intron 25.0%
! ATTAAGAGAAGAACAATCAA+AGG - chr8_1:6868727-6868746 None:intergenic 25.0%
! TTGATTGTTCTTCTCTTAAT+TGG + chr8_1:6868727-6868746 Msa1175510:intron 25.0%
! ATGAACATGTGTTTATAAGT+GGG + chr8_1:6868838-6868857 Msa1175510:intron 25.0%
! TGATACCATATTAGAAATCT+TGG - chr8_1:6869310-6869329 None:intergenic 25.0%
! ATGTACATGAGTTTATAAGT+GGG + chr8_1:6869471-6869490 Msa1175510:intron 25.0%
! TCCTATTCCTATTAAATTGA+TGG + chr8_1:6869573-6869592 Msa1175510:intron 25.0%
! ACCATCAATTTAATAGGAAT+AGG - chr8_1:6869577-6869596 None:intergenic 25.0%
!! AACATATATTTTCTCTTTGC+AGG + chr8_1:6869630-6869649 Msa1175510:intron 25.0%
! TAATTCTTTCTGCTCAAATT+TGG - chr8_1:6869817-6869836 None:intergenic 25.0%
! AAAATCATGTTTAAACTCGT+AGG - chr8_1:6869939-6869958 None:intergenic 25.0%
! ACATGTTTAAGTCATGTAAA+CGG + chr8_1:6870000-6870019 Msa1175510:intron 25.0%
! AATCTTCCATATTCAATTCA+GGG - chr8_1:6870560-6870579 None:intergenic 25.0%
! AAATCTTCCATATTCAATTC+AGG - chr8_1:6870561-6870580 None:intergenic 25.0%
! AACTTTTAAACTATGCACCA+TGG + chr8_1:6866059-6866078 Msa1175510:CDS 30.0%
! ACTTTTAAACTATGCACCAT+GGG + chr8_1:6866060-6866079 Msa1175510:CDS 30.0%
GGTAGAATATTTATCTTACC+CGG - chr8_1:6866091-6866110 None:intergenic 30.0%
! CATGTTTTCCTACTTTAATG+GGG + chr8_1:6866144-6866163 Msa1175510:intron 30.0%
AATGGGGTTATATTAAATGG+TGG + chr8_1:6866160-6866179 Msa1175510:intron 30.0%
!! AAAGGTACCAACATTGTTAT+AGG + chr8_1:6866290-6866309 Msa1175510:CDS 30.0%
ATATTGTCTATAGCTTGAAC+TGG + chr8_1:6866531-6866550 Msa1175510:intron 30.0%
TAACTTGAGAATTATGTCAG+TGG - chr8_1:6866768-6866787 None:intergenic 30.0%
CTACATCAGCATTAGTTAAA+CGG - chr8_1:6866927-6866946 None:intergenic 30.0%
GATCCAAGTCAACAATTTAA+CGG - chr8_1:6866950-6866969 None:intergenic 30.0%
TGTCAAAACACTACGTTTAA+TGG + chr8_1:6866998-6867017 Msa1175510:intron 30.0%
TGTCAAAACGTTACGTTTAA+TGG - chr8_1:6867128-6867147 None:intergenic 30.0%
TTAAATCCACGGTAAATGTT+TGG - chr8_1:6867393-6867412 None:intergenic 30.0%
CAAACATGTGAAAACATGAT+CGG + chr8_1:6867518-6867537 Msa1175510:intron 30.0%
CATGTGTTTGACACTAAAAA+TGG + chr8_1:6867549-6867568 Msa1175510:intron 30.0%
!!! TTTTTCAAAGTTTTGTTGGG+AGG + chr8_1:6867719-6867738 Msa1175510:intron 30.0%
CAAGATATTATTTCACACAG+TGG + chr8_1:6867757-6867776 Msa1175510:intron 30.0%
!!! ATTGCAAATTTTTTTTCGCC+CGG - chr8_1:6867806-6867825 None:intergenic 30.0%
AAATTGCAAAAAATTTGGGC+CGG + chr8_1:6867934-6867953 Msa1175510:intron 30.0%
AACATTCATGTGTGAAATCA+GGG - chr8_1:6868001-6868020 None:intergenic 30.0%
! GTGAAAAAGAGGAAGAATTT+TGG + chr8_1:6868139-6868158 Msa1175510:intron 30.0%
! TTGATCTGGAACAAACATAT+GGG - chr8_1:6868294-6868313 None:intergenic 30.0%
! ATTGATCTGGAACAAACATA+TGG - chr8_1:6868295-6868314 None:intergenic 30.0%
ATATGTTTGTTCCAGATCAA+TGG + chr8_1:6868294-6868313 Msa1175510:intron 30.0%
! TTTATGTCTGTGGTTCTATT+TGG + chr8_1:6868367-6868386 Msa1175510:intron 30.0%
AGCTCTCTTTATTGGTATAT+TGG + chr8_1:6868609-6868628 Msa1175510:intron 30.0%
!!! GTTTATTTTGATTCCTCTCT+TGG + chr8_1:6868775-6868794 Msa1175510:intron 30.0%
! ATGTCATCTTTTGTCTAATG+TGG - chr8_1:6868820-6868839 None:intergenic 30.0%
CATGAACATGTGTTTATAAG+TGG + chr8_1:6868837-6868856 Msa1175510:intron 30.0%
TGATACCATCTTATCAATCA+TGG - chr8_1:6868911-6868930 None:intergenic 30.0%
! AAAGCCTATCTAAGATCTAT+TGG + chr8_1:6868930-6868949 Msa1175510:intron 30.0%
TGACAATGTGTTTATAAGTG+AGG + chr8_1:6869062-6869081 Msa1175510:intron 30.0%
GACAATGTGTTTATAAGTGA+GGG + chr8_1:6869063-6869082 Msa1175510:intron 30.0%
CTCAACCAAGATTTCTAATA+TGG + chr8_1:6869302-6869321 Msa1175510:intron 30.0%
CATGTACATGAGTTTATAAG+TGG + chr8_1:6869470-6869489 Msa1175510:intron 30.0%
TGTACATGAGTTTATAAGTG+GGG + chr8_1:6869472-6869491 Msa1175510:intron 30.0%
CTTAATGGAGAAAATTGAGA+AGG + chr8_1:6869672-6869691 Msa1175510:intron 30.0%
AAAATTGAGAAGGAAACACA+AGG + chr8_1:6869682-6869701 Msa1175510:intron 30.0%
AAATTGAGAAGGAAACACAA+GGG + chr8_1:6869683-6869702 Msa1175510:intron 30.0%
TTTGGAAACATAGCAACAAT+GGG + chr8_1:6869742-6869761 Msa1175510:intron 30.0%
AGGATGTATTCACTTACATA+TGG - chr8_1:6869775-6869794 None:intergenic 30.0%
! AATACATCCTCCAAATGTTT+TGG + chr8_1:6869785-6869804 Msa1175510:intron 30.0%
TTGGAGTTAACCAAAACATT+TGG - chr8_1:6869798-6869817 None:intergenic 30.0%
TTGAGCAGAAAGAATTAGTT+AGG + chr8_1:6869820-6869839 Msa1175510:intron 30.0%
GACTTAAACATGTGACATTT+CGG - chr8_1:6869993-6870012 None:intergenic 30.0%
GACATAATACACCAATTGAT+GGG + chr8_1:6870051-6870070 Msa1175510:intron 30.0%
CAAAAACTCTCACATATGAA+GGG - chr8_1:6870079-6870098 None:intergenic 30.0%
ACAAAAACTCTCACATATGA+AGG - chr8_1:6870080-6870099 None:intergenic 30.0%
TAATAACCACAAAGGGTTTA+AGG + chr8_1:6870332-6870351 Msa1175510:intron 30.0%
! GATTCTTGCTTCATGTTTTT+AGG + chr8_1:6870524-6870543 Msa1175510:intron 30.0%
ATCTTCCATATTCAATTCAG+GGG - chr8_1:6870559-6870578 None:intergenic 30.0%
AGCATAAATTCAATGCTACA+AGG + chr8_1:6870612-6870631 Msa1175510:CDS 30.0%
!!! TTCGTTCGTTGATTCAATTT+TGG + chr8_1:6870710-6870729 Msa1175510:CDS 30.0%
GGTCTGAATCTATAACCTAT+AGG - chr8_1:6865953-6865972 None:intergenic 35.0%
TACCTAAATCACAAGTTGCT+TGG + chr8_1:6865998-6866017 Msa1175510:CDS 35.0%
TACCAAGCAACTTGTGATTT+AGG - chr8_1:6866003-6866022 None:intergenic 35.0%
TGAAATCGGAAGATGGTTAT+TGG + chr8_1:6866234-6866253 Msa1175510:CDS 35.0%
GATCGTGAAATCAAGACAAA+AGG + chr8_1:6866272-6866291 Msa1175510:CDS 35.0%
ATCAACATGAAAAGACCAAC+TGG + chr8_1:6866342-6866361 Msa1175510:CDS 35.0%
TCAACATGAAAAGACCAACT+GGG + chr8_1:6866343-6866362 Msa1175510:CDS 35.0%
! TACTGATTGTGTCACTAAAG+TGG + chr8_1:6866610-6866629 Msa1175510:intron 35.0%
GGGAATTCTGTCAAACTAAA+AGG - chr8_1:6866821-6866840 None:intergenic 35.0%
! TAGCCGTTAAATTGTTGACT+TGG + chr8_1:6866944-6866963 Msa1175510:intron 35.0%
! TTTTTCCAGCTTGTGAAGTT+GGG + chr8_1:6867434-6867453 Msa1175510:intron 35.0%
TTTGAGAGCTTACTATTCCA+CGG - chr8_1:6867474-6867493 None:intergenic 35.0%
! CGATCATGTTTTCACATGTT+TGG - chr8_1:6867520-6867539 None:intergenic 35.0%
AACATTGATATGCGCATTCA+TGG - chr8_1:6867587-6867606 None:intergenic 35.0%
GATATTATTTCACACAGTGG+CGG + chr8_1:6867760-6867779 Msa1175510:intron 35.0%
!!! TTGCAAATTTTTTTTCGCCC+GGG - chr8_1:6867805-6867824 None:intergenic 35.0%
ACATTCATGTGTGAAATCAG+GGG - chr8_1:6868000-6868019 None:intergenic 35.0%
GAACATTCATGTGTGAAATC+AGG - chr8_1:6868002-6868021 None:intergenic 35.0%
CTCCTAAACAATTCGTCTTA+TGG + chr8_1:6868021-6868040 Msa1175510:intron 35.0%
CACCATAAGACGAATTGTTT+AGG - chr8_1:6868026-6868045 None:intergenic 35.0%
CTAAACAATTCGTCTTATGG+TGG + chr8_1:6868024-6868043 Msa1175510:intron 35.0%
AACACTTGAGCTGAATTGTT+TGG + chr8_1:6868055-6868074 Msa1175510:intron 35.0%
ACACTTGAGCTGAATTGTTT+GGG + chr8_1:6868056-6868075 Msa1175510:intron 35.0%
!! TTCCTCTTTTTCACAAGAGT+CGG - chr8_1:6868133-6868152 None:intergenic 35.0%
AAAACAAAAAGTCAAGCCGT+GGG - chr8_1:6868220-6868239 None:intergenic 35.0%
! TTGCAACTCGATCTAGATTT+TGG + chr8_1:6868250-6868269 Msa1175510:intron 35.0%
! TGCAACTCGATCTAGATTTT+GGG + chr8_1:6868251-6868270 Msa1175510:intron 35.0%
!! TGGTTTGTTCGTACTAATGT+TGG + chr8_1:6868321-6868340 Msa1175510:intron 35.0%
TTTATGTTCTGCCATGATTG+TGG + chr8_1:6868344-6868363 Msa1175510:intron 35.0%
ACAGACATAAACCACAATCA+TGG - chr8_1:6868358-6868377 None:intergenic 35.0%
ATGATTGTGGTTTATGTCTG+TGG + chr8_1:6868357-6868376 Msa1175510:intron 35.0%
! TTGAGTATTTTCGTTCGACA+GGG + chr8_1:6868415-6868434 Msa1175510:intron 35.0%
AAGAGAAGAACAATCAAAGG+AGG - chr8_1:6868724-6868743 None:intergenic 35.0%
ACAATCCTCACTTCATAAGT+TGG + chr8_1:6868861-6868880 Msa1175510:intron 35.0%
ACAAACCAACTTATGAAGTG+AGG - chr8_1:6868869-6868888 None:intergenic 35.0%
!! TCACTTCATAAGTTGGTTTG+TGG + chr8_1:6868868-6868887 Msa1175510:intron 35.0%
!! CACTTCATAAGTTGGTTTGT+GGG + chr8_1:6868869-6868888 Msa1175510:intron 35.0%
!! ACTTCATAAGTTGGTTTGTG+GGG + chr8_1:6868870-6868889 Msa1175510:intron 35.0%
CTCAACCATGATTGATAAGA+TGG + chr8_1:6868903-6868922 Msa1175510:intron 35.0%
TGGTCCAATAGATCTTAGAT+AGG - chr8_1:6868937-6868956 None:intergenic 35.0%
ATCATCCATCATTTATGTCC+AGG + chr8_1:6868982-6869001 Msa1175510:intron 35.0%
TTTAACACACCGGTACTATT+GGG - chr8_1:6869015-6869034 None:intergenic 35.0%
AGTCATCTCTCATTCAATGT+GGG - chr8_1:6869042-6869061 None:intergenic 35.0%
AAGTCATCTCTCATTCAATG+TGG - chr8_1:6869043-6869062 None:intergenic 35.0%
!!! ACAAGTTGGTTTTTTGAGGT+TGG + chr8_1:6869099-6869118 Msa1175510:intron 35.0%
!!! TTGGTTTTTTGAGGTTGGTT+AGG + chr8_1:6869104-6869123 Msa1175510:intron 35.0%
AAACTCTTATCAATCGTGGT+TGG - chr8_1:6869131-6869150 None:intergenic 35.0%
CAAGAAACTCTTATCAATCG+TGG - chr8_1:6869135-6869154 None:intergenic 35.0%
TGACCATGTGTTTAAAAGTG+AGG + chr8_1:6869236-6869255 Msa1175510:intron 35.0%
GACCATGTGTTTAAAAGTGA+GGG + chr8_1:6869237-6869256 Msa1175510:intron 35.0%
!! TTCCCTCACTTTTAAACACA+TGG - chr8_1:6869242-6869261 None:intergenic 35.0%
!! GTTTTGTTCGTGGACATAAA+TGG - chr8_1:6869392-6869411 None:intergenic 35.0%
!! GCACTATTATGTTTTGTTCG+TGG - chr8_1:6869402-6869421 None:intergenic 35.0%
CGAACAAAACATAATAGTGC+TGG + chr8_1:6869402-6869421 Msa1175510:intron 35.0%
!! ATAATAGTGCTGGTCATGAT+GGG + chr8_1:6869412-6869431 Msa1175510:intron 35.0%
TGTGTTAAAGAGTCACACTT+CGG + chr8_1:6869436-6869455 Msa1175510:intron 35.0%
ACAATCCTTACATCACAAAC+CGG + chr8_1:6869495-6869514 Msa1175510:intron 35.0%
!! AAAAACCGGTTTGTGATGTA+AGG - chr8_1:6869503-6869522 None:intergenic 35.0%
!!! TACATCACAAACCGGTTTTT+TGG + chr8_1:6869503-6869522 Msa1175510:intron 35.0%
TGTTAGACCACGATTGATAA+GGG + chr8_1:6869529-6869548 Msa1175510:intron 35.0%
TGCTATGTTTCCAAAGATAG+AGG - chr8_1:6869737-6869756 None:intergenic 35.0%
! CTTTGGAAACATAGCAACAA+TGG + chr8_1:6869741-6869760 Msa1175510:intron 35.0%
GAGTTAACCAAAACATTTGG+AGG - chr8_1:6869795-6869814 None:intergenic 35.0%
! GCAAATCTTAGGTTCAAGTT+GGG - chr8_1:6869914-6869933 None:intergenic 35.0%
TGCAAATCTTAGGTTCAAGT+TGG - chr8_1:6869915-6869934 None:intergenic 35.0%
GGACATAATACACCAATTGA+TGG + chr8_1:6870050-6870069 Msa1175510:intron 35.0%
ATTAGACCTTAAACCCTTTG+TGG - chr8_1:6870341-6870360 None:intergenic 35.0%
! GGGTTTAAGGTCTAATTTCA+AGG + chr8_1:6870345-6870364 Msa1175510:intron 35.0%
TTCTTCCCCTGAATTGAATA+TGG + chr8_1:6870551-6870570 Msa1175510:CDS 35.0%
TTTCTTTCCAAAAACACCGA+AGG + chr8_1:6870578-6870597 Msa1175510:CDS 35.0%
ATGCTAAATTCCTCTTCCTT+CGG - chr8_1:6870597-6870616 None:intergenic 35.0%
!! GTTGATTCAATTTTGGACGA+TGG + chr8_1:6870717-6870736 Msa1175510:CDS 35.0%
ACCTCAACTTCAATGCCTAT+AGG + chr8_1:6865935-6865954 Msa1175510:CDS 40.0%
!! ACCTATAGGCATTGAAGTTG+AGG - chr8_1:6865939-6865958 None:intergenic 40.0%
CTCACAAGTTCTTGATCTGT+TGG - chr8_1:6865974-6865993 None:intergenic 40.0%
! CGTGTTATTAGCATTGTCCA+AGG + chr8_1:6866211-6866230 Msa1175510:intron 40.0%
AGCATTGTCCAAGGTGAAAT+CGG + chr8_1:6866220-6866239 Msa1175510:intron 40.0%
GATGGTTATTGGAAGATCAC+TGG + chr8_1:6866245-6866264 Msa1175510:CDS 40.0%
ATACTGATGCATAACCCAGT+TGG - chr8_1:6866360-6866379 None:intergenic 40.0%
TATTTCACCGTTACCCATGT+TGG + chr8_1:6866895-6866914 Msa1175510:intron 40.0%
TAAACGGATACTGCCAACAT+GGG - chr8_1:6866911-6866930 None:intergenic 40.0%
TTAAACGGATACTGCCAACA+TGG - chr8_1:6866912-6866931 None:intergenic 40.0%
!! GATGCCTTTTAAAAGCTCCA+GGG - chr8_1:6867151-6867170 None:intergenic 40.0%
TAACTGACTAGCAAGGGTAA+CGG + chr8_1:6867222-6867241 Msa1175510:intron 40.0%
AAAGGACCAAACATTTACCG+TGG + chr8_1:6867384-6867403 Msa1175510:intron 40.0%
! GTTTTTCCAGCTTGTGAAGT+TGG + chr8_1:6867433-6867452 Msa1175510:intron 40.0%
! TTTTCCAGCTTGTGAAGTTG+GGG + chr8_1:6867435-6867454 Msa1175510:intron 40.0%
ACACTAAAAATGGCGTTCCT+CGG + chr8_1:6867559-6867578 Msa1175510:intron 40.0%
! GCCAGGATTTTTATAAAGCC+CGG + chr8_1:6867784-6867803 Msa1175510:intron 40.0%
! CCAGGATTTTTATAAAGCCC+GGG + chr8_1:6867785-6867804 Msa1175510:intron 40.0%
ATCCGACTCTTGTGAAAAAG+AGG + chr8_1:6868128-6868147 Msa1175510:intron 40.0%
GAAATAGTATACTCCACAGG+GGG - chr8_1:6868198-6868217 None:intergenic 40.0%
GGAAATAGTATACTCCACAG+GGG - chr8_1:6868199-6868218 None:intergenic 40.0%
GGGAAATAGTATACTCCACA+GGG - chr8_1:6868200-6868219 None:intergenic 40.0%
TGGGAAATAGTATACTCCAC+AGG - chr8_1:6868201-6868220 None:intergenic 40.0%
GTGGAGTATACTATTTCCCA+CGG + chr8_1:6868201-6868220 Msa1175510:intron 40.0%
CAAAACAAAAAGTCAAGCCG+TGG - chr8_1:6868221-6868240 None:intergenic 40.0%
!! CACGGCTTGACTTTTTGTTT+TGG + chr8_1:6868219-6868238 Msa1175510:intron 40.0%
TGTTCCAGATCAATGGTGTT+TGG + chr8_1:6868301-6868320 Msa1175510:intron 40.0%
CAAACCAAACACCATTGATC+TGG - chr8_1:6868308-6868327 None:intergenic 40.0%
! GTTGAGTATTTTCGTTCGAC+AGG + chr8_1:6868414-6868433 Msa1175510:intron 40.0%
AGAAGAACAATCAAAGGAGG+AGG - chr8_1:6868721-6868740 None:intergenic 40.0%
TGAACAGAAACAACCAAGAG+AGG - chr8_1:6868791-6868810 None:intergenic 40.0%
TGCTATCATGTTTCCGCTAT+CGG + chr8_1:6868959-6868978 Msa1175510:intron 40.0%
!! AATGATGGATGATCCGATAG+CGG - chr8_1:6868975-6868994 None:intergenic 40.0%
TGGTTCCTGGACATAAATGA+TGG - chr8_1:6868990-6869009 None:intergenic 40.0%
CTTTAACACACCGGTACTAT+TGG - chr8_1:6869016-6869035 None:intergenic 40.0%
AAAAACCAACTTGTGAGGTG+AGG - chr8_1:6869093-6869112 None:intergenic 40.0%
CCTCAAAAAACCAACTTGTG+AGG - chr8_1:6869098-6869117 None:intergenic 40.0%
!!! CCTCACAAGTTGGTTTTTTG+AGG + chr8_1:6869095-6869114 Msa1175510:intron 40.0%
CCATCTCTTATCCCACTATT+GGG - chr8_1:6869214-6869233 None:intergenic 40.0%
ACCATCTCTTATCCCACTAT+TGG - chr8_1:6869215-6869234 None:intergenic 40.0%
AGCCTTCGTTAAGATCCATT+GGG + chr8_1:6869331-6869350 Msa1175510:intron 40.0%
CTGCTATTAGGTTTCGCTAT+CGG + chr8_1:6869358-6869377 Msa1175510:intron 40.0%
TGCTATTAGGTTTCGCTATC+GGG + chr8_1:6869359-6869378 Msa1175510:intron 40.0%
TGTTCGTGGACATAAATGGT+CGG - chr8_1:6869388-6869407 None:intergenic 40.0%
!! CATAATAGTGCTGGTCATGA+TGG + chr8_1:6869411-6869430 Msa1175510:intron 40.0%
!! TAATAGTGCTGGTCATGATG+GGG + chr8_1:6869413-6869432 Msa1175510:intron 40.0%
GTCTAACACAGCCAAAAAAC+CGG - chr8_1:6869517-6869536 None:intergenic 40.0%
GTGTTAGACCACGATTGATA+AGG + chr8_1:6869528-6869547 Msa1175510:intron 40.0%
CAAGAAACCCTTATCAATCG+TGG - chr8_1:6869539-6869558 None:intergenic 40.0%
!! TATTGTCCTGTGTCGCTTAA+TGG + chr8_1:6869657-6869676 Msa1175510:intron 40.0%
! TTTTCTCCATTAAGCGACAC+AGG - chr8_1:6869666-6869685 None:intergenic 40.0%
ACTCGTAGGTTGCAAATCTT+AGG - chr8_1:6869925-6869944 None:intergenic 40.0%
TATGAAGGGATCCCATCAAT+TGG - chr8_1:6870065-6870084 None:intergenic 40.0%
CTTGAGAACGAAAACAGCAT+TGG + chr8_1:6870642-6870661 Msa1175510:CDS 40.0%
TCTACCTGAAGCTCTTGAAA+AGG + chr8_1:6870671-6870690 Msa1175510:CDS 40.0%
CTATGCACCATGGGATTTAC+CGG + chr8_1:6866069-6866088 Msa1175510:CDS 45.0%
TATGCACCATGGGATTTACC+GGG + chr8_1:6866070-6866089 Msa1175510:CDS 45.0%
ATCTTACCCGGTAAATCCCA+TGG - chr8_1:6866079-6866098 None:intergenic 45.0%
TCCAAGGTGAAATCGGAAGA+TGG + chr8_1:6866227-6866246 Msa1175510:CDS 45.0%
ACCATCTTCCGATTTCACCT+TGG - chr8_1:6866231-6866250 None:intergenic 45.0%
GATACTGCCAACATGGGTAA+CGG - chr8_1:6866905-6866924 None:intergenic 45.0%
!! AACGTAACGTTTTGACACCC+TGG + chr8_1:6867131-6867150 Msa1175510:intron 45.0%
! GACACCCTGGAGCTTTTAAA+AGG + chr8_1:6867144-6867163 Msa1175510:intron 45.0%
!! CGATGCCTTTTAAAAGCTCC+AGG - chr8_1:6867152-6867171 None:intergenic 45.0%
! GGGGATCACAATTTTTACCG+TGG + chr8_1:6867454-6867473 Msa1175510:intron 45.0%
! CCCGGGCTTTATAAAAATCC+TGG - chr8_1:6867788-6867807 None:intergenic 45.0%
TTCATGTGTGAAATCAGGGG+CGG - chr8_1:6867997-6868016 None:intergenic 45.0%
!! TTGGTTTGTGGGGTTGTGTT+AGG + chr8_1:6868880-6868899 Msa1175510:intron 45.0%
AGCGGAAACATGATAGCATG+TGG - chr8_1:6868957-6868976 None:intergenic 45.0%
TGTGGGACTCTTTAACACAC+CGG - chr8_1:6869025-6869044 None:intergenic 45.0%
! GCTTGGTTCGTGGACATAAA+TGG - chr8_1:6869192-6869211 None:intergenic 45.0%
TCTTATCCCACTATTGGGCT+TGG - chr8_1:6869209-6869228 None:intergenic 45.0%
! CCCAATAGTGGGATAAGAGA+TGG + chr8_1:6869211-6869230 Msa1175510:intron 45.0%
CTAACACAACCCCACGAAAT+CGG - chr8_1:6869281-6869300 None:intergenic 45.0%
GAGCCTTCGTTAAGATCCAT+TGG + chr8_1:6869330-6869349 Msa1175510:intron 45.0%
GACCCAATGGATCTTAACGA+AGG - chr8_1:6869336-6869355 None:intergenic 45.0%
CGATAGCGAAACCTAATAGC+AGG - chr8_1:6869360-6869379 None:intergenic 45.0%
GTTTCCAAAGATAGAGGCAG+CGG - chr8_1:6869731-6869750 None:intergenic 45.0%
TCCAAAAACACCGAAGGAAG+AGG + chr8_1:6870584-6870603 Msa1175510:CDS 45.0%
!!! TCCTCTTCCTTCGGTGTTTT+TGG - chr8_1:6870588-6870607 None:intergenic 45.0%
!! CTGTCCTTTTCAAGAGCTTC+AGG - chr8_1:6870678-6870697 None:intergenic 45.0%
!!! AAGTTTTAAAAATTTTAAAT+AGG + chr8_1:6866854-6866873 Msa1175510:intron 5.0%
TGATCCCCAACTTCACAAGC+TGG - chr8_1:6867442-6867461 None:intergenic 50.0%
TATGCGCATTCATGGAACCG+AGG - chr8_1:6867579-6867598 None:intergenic 50.0%
AAAAAATTTGGGCCGGAGCC+CGG + chr8_1:6867941-6867960 Msa1175510:intron 50.0%
TGAAATCAGGGGCGGTTCTA+AGG - chr8_1:6867989-6868008 None:intergenic 50.0%
! GTACTATTGGGCTTGGTTCC+TGG - chr8_1:6869003-6869022 None:intergenic 50.0%
GGAACCAAGCCCAATAGTAC+CGG + chr8_1:6869003-6869022 Msa1175510:intron 50.0%
GCAATCCTCACCTCACAAGT+TGG + chr8_1:6869085-6869104 Msa1175510:intron 50.0%
CACGAACCAAGCCCAATAGT+GGG + chr8_1:6869200-6869219 Msa1175510:intron 50.0%
! ACCTCACAAGCCGATTTCGT+GGG + chr8_1:6869268-6869287 Msa1175510:intron 50.0%
CCTAATAGCAGGTGACCCAA+TGG - chr8_1:6869349-6869368 None:intergenic 50.0%
CCATTGGGTCACCTGCTATT+AGG + chr8_1:6869346-6869365 Msa1175510:intron 50.0%
AAAAATTTGGGCCGGAGCCC+GGG + chr8_1:6867942-6867961 Msa1175510:intron 55.0%
CGGTTTGAAAAGACCCCCTG+TGG + chr8_1:6868182-6868201 Msa1175510:intron 55.0%
CACACCGGTACTATTGGGCT+TGG - chr8_1:6869010-6869029 None:intergenic 55.0%
! CCACTATTGGGCTTGGTTCG+TGG - chr8_1:6869202-6869221 None:intergenic 55.0%
CCACGAACCAAGCCCAATAG+TGG + chr8_1:6869199-6869218 Msa1175510:intron 55.0%
!! CGAAATCGGCTTGTGAGGTG+AGG - chr8_1:6869267-6869286 None:intergenic 55.0%
! CACCTCACAAGCCGATTTCG+TGG + chr8_1:6869267-6869286 Msa1175510:intron 55.0%
! CCTCACAAGCCGATTTCGTG+GGG + chr8_1:6869269-6869288 Msa1175510:intron 55.0%
CAAAGATAGAGGCAGCGGCA+TGG - chr8_1:6869726-6869745 None:intergenic 55.0%
CATGCCGCTGCCTCTATCTT+TGG + chr8_1:6869724-6869743 Msa1175510:intron 55.0%
! TTTCACACAGTGGCGGAGCC+AGG + chr8_1:6867767-6867786 Msa1175510:intron 60.0%
!! CCCCACGAAATCGGCTTGTG+AGG - chr8_1:6869272-6869291 None:intergenic 60.0%
!! TCAGGGGCGGTTCTAAGGCC+CGG - chr8_1:6867984-6868003 None:intergenic 65.0%
!! TTCTAAGGCCCGGCGAGCCC+GGG - chr8_1:6867974-6867993 None:intergenic 70.0%
!! GTTCTAAGGCCCGGCGAGCC+CGG - chr8_1:6867975-6867994 None:intergenic 70.0%
! CCGGGCACTCGCCCGGGCTC+CGG - chr8_1:6867956-6867975 None:intergenic 85.0%
! CCGGAGCCCGGGCGAGTGCC+CGG + chr8_1:6867953-6867972 Msa1175510:intron 85.0%
! CGGAGCCCGGGCGAGTGCCC+GGG + chr8_1:6867954-6867973 Msa1175510:intron 85.0%
! GCGAGCCCGGGCACTCGCCC+GGG - chr8_1:6867962-6867981 None:intergenic 85.0%
! GGCGAGCCCGGGCACTCGCC+CGG - chr8_1:6867963-6867982 None:intergenic 85.0%
! GGGCGAGTGCCCGGGCTCGC+CGG + chr8_1:6867962-6867981 Msa1175510:intron 85.0%
! GGCGAGTGCCCGGGCTCGCC+GGG + chr8_1:6867963-6867982 Msa1175510:intron 85.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 6865929 6870767 6865929 ID=Msa1175510;Name=Msa1175510
chr8_1 mRNA 6865929 6870767 6865929 ID=Msa1175510-mRNA-1;Parent=Msa1175510;Name=Msa1175510-mRNA-1;_AED=0.11;_eAED=0.12;_QI=0|0|0|1|1|1|3|0|188
chr8_1 exon 6865929 6866091 6865929 ID=Msa1175510-mRNA-1:exon:2488;Parent=Msa1175510-mRNA-1
chr8_1 exon 6866222 6866403 6866222 ID=Msa1175510-mRNA-1:exon:2489;Parent=Msa1175510-mRNA-1
chr8_1 exon 6870546 6870767 6870546 ID=Msa1175510-mRNA-1:exon:2490;Parent=Msa1175510-mRNA-1
chr8_1 CDS 6865929 6866091 6865929 ID=Msa1175510-mRNA-1:cds;Parent=Msa1175510-mRNA-1
chr8_1 CDS 6866222 6866403 6866222 ID=Msa1175510-mRNA-1:cds;Parent=Msa1175510-mRNA-1
chr8_1 CDS 6870546 6870767 6870546 ID=Msa1175510-mRNA-1:cds;Parent=Msa1175510-mRNA-1
Gene Sequence

>Msa1175510

ATGGAGACCTCAACTTCAATGCCTATAGGTTATAGATTCAGACCAACAGATCAAGAACTTGTGAGTCACTACCTAAATCACAAGTTGCTTGGTAATGATTCTGTTGTCAACAACATAATACCTCATGTTCAACTTTTAAACTATGCACCATGGGATTTACCGGCATTGTCCAAGGTGAAATCGGAAGATGGTTATTGGAAGATCACTGGTAAAGATCGTGAAATCAAGACAAAAGGTACCAACATTGTTATAGGCATAAAAAAGACTCTTGTTTTCTATGGTAATCAACATGAAAAGACCAACTGGGTTATGCATCAGTATCATTCAAACTTTTCTGATAACAAGGTATGTTCTTCCCCTGAATTGAATATGGAAGATTTCTTTCCAAAAACACCGAAGGAAGAGGAATTTAGCATAAATTCAATGCTACAAGGTGCATCTCTTGAGAACGAAAACAGCATTGGACATAATCTACCTGAAGCTCTTGAAAAGGACAGTGCTGATGATTCTTCGTTCGTTGATTCAATTTTGGACGATGGTGTTTCAATTAGTGAAGAAACAAGTTAA
Protein sequence

>Msa1175510

METSTSMPIGYRFRPTDQELVSHYLNHKLLGNDSVVNNIIPHVQLLNYAPWDLPALSKVKSEDGYWKITGKDREIKTKGTNIVIGIKKTLVFYGNQHEKTNWVMHQYHSNFSDNKVCSSPELNMEDFFPKTPKEEEFSINSMLQGASLENENSIGHNLPEALEKDSADDSSFVDSILDDGVSISEETS*