AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1176440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1176440 MtrunA17_Chr7g0275821 71.250 240 51 7 368 600 189 417 1.72e-95 299
Msa1176440 MtrunA17_Chr7g0275821 59.091 176 57 4 12 173 8 182 2.35e-59 204
Msa1176440 MtrunA17_Chr7g0260841 35.235 596 270 21 14 595 9 502 1.16e-83 270
Msa1176440 MtrunA17_Chr8g0366751 44.700 217 97 5 400 594 424 639 2.35e-44 167
Msa1176440 MtrunA17_Chr8g0366751 35.714 196 108 9 51 241 52 234 2.50e-21 98.2
Msa1176440 MtrunA17_Chr5g0411341 43.243 222 101 4 396 594 451 670 9.41e-43 163
Msa1176440 MtrunA17_Chr5g0411341 31.343 268 154 8 51 296 43 302 3.49e-22 101
Msa1176440 MtrunA17_Chr8g0346161 39.535 172 95 1 415 577 271 442 5.56e-34 135
Msa1176440 MtrunA17_Chr8g0346151 41.799 189 96 4 415 590 259 446 8.33e-34 134
Msa1176440 MtrunA17_Chr5g0411331 31.472 197 127 4 51 247 26 214 1.33e-21 94.7
Msa1176440 MtrunA17_Chr8g0386921 33.149 181 107 5 66 239 14 187 2.76e-20 95.1
Msa1176440 MtrunA17_Chr8g0386921 51.389 72 35 0 432 503 403 474 7.31e-15 77.8
Msa1176440 MtrunA17_Chr1g0185621 28.788 198 123 6 50 241 8 193 8.05e-19 90.5
Msa1176440 MtrunA17_Chr1g0185621 55.000 60 27 0 435 494 475 534 1.75e-14 76.6
Msa1176440 MtrunA17_Chr2g0280161 32.353 136 83 2 435 561 177 312 7.20e-17 82.4
Msa1176440 MtrunA17_Chr4g0049331 36.923 130 67 3 433 547 133 262 1.42e-16 80.9
Msa1176440 MtrunA17_Chr5g0400671 39.252 107 60 2 436 537 155 261 8.58e-16 79.0
Msa1176440 MtrunA17_Chr5g0400661 30.405 148 82 3 435 561 149 296 3.67e-15 77.0
Msa1176440 MtrunA17_Chr3g0131691 37.500 120 65 1 433 542 296 415 6.03e-15 77.8
Msa1176440 MtrunA17_Chr2g0333621 29.221 154 81 3 412 537 96 249 8.32e-15 75.9
Msa1176440 MtrunA17_Chr4g0033391 30.286 175 101 3 414 580 136 297 9.56e-15 75.9
Msa1176440 MtrunA17_Chr1g0211081 41.489 94 49 2 400 493 288 375 3.23e-14 75.5
Msa1176440 MtrunA17_Chr7g0225901 44.318 88 44 2 412 499 70 152 4.31e-14 72.8
Msa1176440 MtrunA17_Chr4g0054571 35.537 121 63 2 435 540 150 270 4.88e-14 73.6
Msa1176440 MtrunA17_Chr4g0054561 34.146 123 62 3 423 535 147 260 5.39e-14 73.6
Msa1176440 MtrunA17_Chr2g0320291 27.011 174 113 4 415 582 121 286 6.75e-14 73.2
Msa1176440 MtrunA17_Chr2g0307141 29.310 174 108 3 435 596 183 353 8.25e-14 73.2
Msa1176440 MtrunA17_Chr5g0400621 27.922 154 103 1 435 580 157 310 8.28e-14 73.2
Msa1176440 MtrunA17_Chr6g0468461 51.562 64 31 0 435 498 173 236 9.32e-14 73.2
Msa1176440 MtrunA17_Chr4g0054581 44.872 78 37 1 421 498 190 261 9.53e-14 73.2
Msa1176440 MtrunA17_Chr2g0307131 28.509 228 138 7 382 596 134 349 9.78e-14 73.2
Msa1176440 MtrunA17_Chr4g0028071 32.353 170 100 7 439 593 153 322 1.01e-13 72.8
Msa1176440 MtrunA17_Chr4g0033281 46.753 77 31 1 414 490 142 208 1.11e-13 72.8
Msa1176440 MtrunA17_Chr2g0298751 29.268 164 95 4 436 578 53 216 1.88e-13 70.5
Msa1176440 MtrunA17_Chr4g0033291 46.753 77 31 1 414 490 144 210 2.26e-13 71.6
Msa1176440 MtrunA17_Chr6g0468491 29.221 154 93 2 435 576 137 286 3.18e-13 70.9
Msa1176440 MtrunA17_Chr7g0252041 53.333 60 28 0 433 492 284 343 5.64e-13 71.6
Msa1176440 MtrunA17_Chr2g0333641 31.405 121 76 3 423 537 126 245 3.16e-12 67.8
Msa1176440 MtrunA17_Chr4g0002781 38.961 77 43 1 421 497 164 236 3.83e-12 68.2
Msa1176440 MtrunA17_Chr5g0400641 26.797 153 90 2 439 576 2 147 4.43e-12 64.7
Msa1176440 MtrunA17_Chr2g0333611 24.405 168 120 2 436 596 46 213 5.83e-12 65.5
Msa1176440 MtrunA17_Chr4g0054551 48.276 58 30 0 431 488 151 208 7.30e-12 67.0
Msa1176440 MtrunA17_Chr4g0002801 37.662 77 44 1 421 497 142 214 1.42e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1176440 AT1G01260.1 48.357 639 223 17 9 600 4 582 0.0 528
Msa1176440 AT1G01260.2 48.357 639 223 17 9 600 4 582 0.0 528
Msa1176440 AT1G01260.3 48.357 639 223 17 9 600 4 582 0.0 528
Msa1176440 AT2G46510.1 45.928 614 234 15 13 599 8 550 3.33e-168 491
Msa1176440 AT4G17880.1 31.856 609 346 16 12 597 23 585 2.01e-75 251
Msa1176440 AT1G32640.1 33.505 582 319 16 51 593 66 618 3.09e-75 252
Msa1176440 AT4G16430.1 44.444 234 101 7 374 595 251 467 3.30e-51 183
Msa1176440 AT4G16430.1 38.610 259 136 9 9 260 2 244 5.18e-41 155
Msa1176440 AT5G46760.1 45.413 218 103 5 390 593 369 584 1.59e-46 173
Msa1176440 AT5G46830.1 27.395 522 285 14 71 577 49 491 4.51e-36 142
Msa1176440 AT4G09820.1 31.351 185 113 4 63 241 25 201 8.40e-21 96.7
Msa1176440 AT4G09820.1 48.649 74 27 1 415 488 354 416 5.91e-14 75.1
Msa1176440 AT4G00870.1 50.549 91 35 1 420 500 224 314 1.31e-19 92.0
Msa1176440 AT5G57150.1 33.333 186 101 5 415 583 40 219 1.79e-19 88.6
Msa1176440 AT5G41315.3 30.583 206 117 5 51 241 17 211 1.79e-19 92.8
Msa1176440 AT5G41315.3 45.070 71 39 0 429 499 440 510 1.93e-12 70.5
Msa1176440 AT5G41315.2 30.583 206 117 5 51 241 20 214 1.93e-19 92.8
Msa1176440 AT5G41315.2 45.070 71 39 0 429 499 443 513 1.94e-12 70.5
Msa1176440 AT5G41315.1 30.583 206 117 5 51 241 12 206 1.98e-19 92.8
Msa1176440 AT5G41315.1 45.070 71 39 0 429 499 435 505 2.02e-12 70.5
Msa1176440 AT5G57150.5 32.222 180 99 4 415 577 40 213 6.42e-19 86.3
Msa1176440 AT5G57150.4 32.222 180 99 4 415 577 41 214 8.79e-19 87.0
Msa1176440 AT1G63650.3 28.767 219 127 7 51 254 11 215 8.81e-19 90.5
Msa1176440 AT1G63650.3 47.692 65 34 0 435 499 405 469 3.22e-13 73.2
Msa1176440 AT1G63650.1 28.767 219 127 7 51 254 11 215 8.81e-19 90.5
Msa1176440 AT1G63650.1 47.692 65 34 0 435 499 405 469 3.22e-13 73.2
Msa1176440 AT1G63650.2 28.767 219 127 7 51 254 11 215 8.81e-19 90.5
Msa1176440 AT1G63650.2 47.692 65 34 0 435 499 405 469 3.22e-13 73.2
Msa1176440 AT5G57150.2 33.333 180 97 5 415 577 41 214 9.92e-19 85.9
Msa1176440 AT5G57150.3 33.333 180 97 5 415 577 41 214 1.18e-18 85.5
Msa1176440 AT4G00480.3 29.082 196 110 5 66 241 31 217 1.73e-17 86.3
Msa1176440 AT4G00480.1 29.082 196 110 5 66 241 31 217 2.28e-17 85.9
Msa1176440 AT4G00480.2 29.082 196 110 5 66 241 31 217 3.65e-17 85.5
Msa1176440 AT4G37850.2 29.648 199 120 3 402 585 119 312 1.13e-16 82.0
Msa1176440 AT4G37850.1 29.648 199 120 3 402 585 119 312 1.13e-16 82.0
Msa1176440 AT4G29930.5 29.268 164 101 5 419 577 43 196 2.63e-15 76.3
Msa1176440 AT4G29930.2 28.994 169 105 5 419 582 43 201 3.36e-15 75.5
Msa1176440 AT4G29930.6 28.144 167 112 3 419 582 43 204 4.63e-15 75.5
Msa1176440 AT4G29930.4 58.621 58 24 0 437 494 56 113 3.58e-14 71.6
Msa1176440 AT4G29930.3 48.684 76 34 1 419 494 43 113 4.00e-14 73.2
Msa1176440 AT4G29930.7 48.684 76 34 1 419 494 43 113 4.00e-14 73.2
Msa1176440 AT4G29930.1 48.684 76 34 1 419 494 43 113 4.96e-14 72.8
Msa1176440 AT3G26744.6 37.500 120 65 1 433 542 305 424 7.77e-14 74.7
Msa1176440 AT3G26744.1 37.500 120 65 1 433 542 305 424 9.71e-14 74.3
Msa1176440 AT3G26744.4 37.500 120 65 1 433 542 305 424 9.71e-14 74.3
Msa1176440 AT3G26744.2 37.500 120 65 1 433 542 305 424 9.71e-14 74.3
Msa1176440 AT2G22750.1 36.111 144 57 4 418 537 109 241 9.88e-14 72.8
Msa1176440 AT2G22750.4 36.111 144 57 4 418 537 137 269 9.97e-14 73.2
Msa1176440 AT2G22750.5 36.111 144 57 4 418 537 133 265 1.02e-13 73.2
Msa1176440 AT2G22750.3 36.111 144 57 4 418 537 137 269 1.02e-13 73.2
Msa1176440 AT2G22750.2 36.111 144 57 4 418 537 109 241 1.05e-13 72.8
Msa1176440 AT1G12860.2 49.333 75 34 1 433 507 265 335 1.60e-13 73.6
Msa1176440 AT1G12860.1 49.333 75 34 1 433 507 265 335 1.81e-13 73.2
Msa1176440 AT2G22760.2 33.333 117 59 3 436 537 120 232 2.01e-13 71.6
Msa1176440 AT2G22760.1 33.333 117 59 3 436 537 120 232 2.24e-13 71.6
Msa1176440 AT2G16910.1 53.846 65 29 1 439 503 318 381 2.93e-13 73.2
Msa1176440 AT2G28160.1 30.380 158 96 5 439 583 135 291 2.01e-12 68.9
Msa1176440 AT2G28160.2 30.380 158 96 5 439 583 135 291 2.22e-12 68.9
Msa1176440 AT2G22770.1 50.000 66 30 1 429 491 123 188 4.43e-12 68.2
Msa1176440 AT1G10610.1 26.452 155 102 3 436 578 265 419 8.00e-11 65.1
Msa1176440 AT1G10610.2 26.452 155 102 3 436 578 235 389 9.76e-11 64.7

Find 117 sgRNAs with CRISPR-Local

Find 314 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGGAAAAGGAACAGAATT+TGG 0.212083 8_1:-8539499 Msa1176440:CDS
CTATAAGGAGTTGGTTTCTT+TGG 0.221769 8_1:+8537852 None:intergenic
GATGATGACAATTATGCTTT+TGG 0.241708 8_1:-8539390 Msa1176440:CDS
GTAGCTCTGATTATTGTGTT+AGG 0.270853 8_1:-8539233 Msa1176440:CDS
CTGATGTTGAAGCTTCTTGC+AGG 0.272050 8_1:-8538434 Msa1176440:CDS
GGTTCTACCCTTCCATTTGC+TGG 0.277893 8_1:+8538357 None:intergenic
ATGAAGATTGAAGTGGGTTT+AGG 0.280941 8_1:-8539801 Msa1176440:CDS
GAAACTTGCTATTAGGAAAA+TGG 0.287769 8_1:-8538845 Msa1176440:CDS
TTCGGGAGAAACTTGCTATT+AGG 0.295924 8_1:-8538852 Msa1176440:CDS
AAGTGGGTTTAGGAGGAAAT+AGG 0.302141 8_1:-8539791 Msa1176440:CDS
CGAATTTCTCAGTCAAAGTT+TGG 0.303426 8_1:-8539570 Msa1176440:CDS
TGATTTAGGTGTTGTTGAAT+TGG 0.318577 8_1:-8539160 Msa1176440:CDS
TTGTCCATCTTGGAAATATT+CGG 0.320643 8_1:+8538258 None:intergenic
GAGGAAAAGGAACAGAATTT+GGG 0.321673 8_1:-8539498 Msa1176440:CDS
CCAACTTTCAAACAAGAAAA+AGG 0.322553 8_1:+8539009 None:intergenic
CAAAGATGCTTTGTCCATCT+TGG 0.334458 8_1:+8538248 None:intergenic
CGACTCAACGATATCGATTT+CGG 0.339702 8_1:+8537972 None:intergenic
CTTGAACCCTGAACACCATT+TGG 0.342953 8_1:+8538772 None:intergenic
CAGAAGCTTCATGCGACATT+TGG 0.343871 8_1:-8539420 Msa1176440:CDS
TCAGTCAAAGTTTGGTGATT+GGG 0.345613 8_1:-8539562 Msa1176440:CDS
GTTTAGGAGGAAATAGGTTA+TGG 0.348725 8_1:-8539785 Msa1176440:CDS
CAAATCCTGCCCGAAAATCT+TGG 0.349356 8_1:+8538891 None:intergenic
GTTGGGGAGATGGGTGTTGT+AGG 0.351229 8_1:-8539533 Msa1176440:CDS
TAGCTCTGATTATTGTGTTA+GGG 0.371392 8_1:-8539232 Msa1176440:CDS
CTCAGTCAAAGTTTGGTGAT+TGG 0.372558 8_1:-8539563 Msa1176440:CDS
TTTCGGTGTCTTTGAATGAT+TGG 0.372935 8_1:+8537989 None:intergenic
CCTGGTCCACCATGTCCATT+AGG 0.373360 8_1:+8539309 None:intergenic
GGCTTTCTGCCACGTTTCCT+TGG 0.373396 8_1:+8538378 None:intergenic
GAATATGAACTATGGCTATA+AGG 0.379179 8_1:+8537837 None:intergenic
ATGGACAAAGCATCTTTGTT+AGG 0.382051 8_1:-8538243 Msa1176440:CDS
AAGAACAATAGTAATAATAA+TGG 0.401905 8_1:-8538976 Msa1176440:CDS
GATTTAGGTGTTGTTGAATT+GGG 0.403406 8_1:-8539159 Msa1176440:CDS
CTCTGCCTCCACATGATTGA+GGG 0.404175 8_1:+8538335 None:intergenic
CCTCACACCGTTTGCTAGCT+CGG 0.409915 8_1:+8538572 None:intergenic
GAATCCGAGAGAGAGCAATT+AGG 0.414650 8_1:-8538171 Msa1176440:CDS
TCTCTGCCTCCACATGATTG+AGG 0.421326 8_1:+8538334 None:intergenic
CATAGTAGAGTTGAGGGGAT+TGG 0.428756 8_1:-8538919 Msa1176440:CDS
GCAGCTTGAATATCGATATC+AGG 0.436804 8_1:+8538075 None:intergenic
TTGCATCTGGGAAGCATTTG+TGG 0.438927 8_1:-8539278 Msa1176440:CDS
CAACACAATCATCGTTTGCT+AGG 0.442496 8_1:-8539077 Msa1176440:CDS
GAGATCAATTGTTGGTGAAT+CGG 0.451925 8_1:-8538463 Msa1176440:CDS
TTGGGTTCTGTTAGAACGGT+TGG 0.453833 8_1:-8539141 Msa1176440:CDS
AAATCCTGCCCGAAAATCTT+GGG 0.454281 8_1:+8538892 None:intergenic
ATCCTAGTCCCTCCGATGAT+AGG 0.458410 8_1:-8538404 Msa1176440:CDS
AATAATCATAGTAGAGTTGA+GGG 0.462171 8_1:-8538925 Msa1176440:CDS
CCTAATGGACATGGTGGACC+AGG 0.463512 8_1:-8539309 Msa1176440:CDS
GGTTAAGTCGATTTCATCGT+TGG 0.463827 8_1:-8539055 Msa1176440:CDS
AAGCTTCATGCGACATTTGG+TGG 0.467090 8_1:-8539417 Msa1176440:CDS
CGTGGCAGAAAGCCAGCAAA+TGG 0.467789 8_1:-8538369 Msa1176440:CDS
CTATTAGGAAAATGGATGAT+AGG 0.467799 8_1:-8538837 Msa1176440:CDS
CATGGGGAGGCCGTCCGAAT+GGG 0.483663 8_1:-8538810 Msa1176440:CDS
GAACTATGGCTATAAGGAGT+TGG 0.485125 8_1:+8537843 None:intergenic
CTAACGTTTCGAATTTCAGT+TGG 0.492616 8_1:-8539611 Msa1176440:CDS
AAATGGATGATAGGCAATCA+TGG 0.495557 8_1:-8538828 Msa1176440:CDS
CTCCTATCATCGGAGGGACT+AGG 0.496928 8_1:+8538402 None:intergenic
CCTTCAGTGAGATCAATTGT+TGG 0.497365 8_1:-8538471 Msa1176440:CDS
GGAGGCCGTCCGAATGGGAA+TGG 0.498948 8_1:-8538805 Msa1176440:CDS
AAGATTGAAGTGGGTTTAGG+AGG 0.500123 8_1:-8539798 Msa1176440:CDS
TTTCCTTGGCCTCCTATCAT+CGG 0.501052 8_1:+8538392 None:intergenic
AAACTAATTCCATTCCCATT+CGG 0.503424 8_1:+8538796 None:intergenic
TGATGTTGAAGCTTCTTGCA+GGG 0.510338 8_1:-8538433 Msa1176440:CDS
GGAACCTAAAGAGGAGGAAA+AGG 0.510397 8_1:-8539511 Msa1176440:CDS
TCATGGGGAGGCCGTCCGAA+TGG 0.512768 8_1:-8538811 Msa1176440:CDS
AACACAATCATCGTTTGCTA+GGG 0.513978 8_1:-8539076 Msa1176440:CDS
AGCTGCACAAGATGGAGTTA+TGG 0.518699 8_1:-8538058 Msa1176440:CDS
CTTCTCCTTTGTCAGCTGCT+CGG 0.521163 8_1:+8537891 None:intergenic
TTGGGGAGATGGGTGTTGTA+GGG 0.522649 8_1:-8539532 Msa1176440:CDS
GATTCCTAATTGCTCTCTCT+CGG 0.526835 8_1:+8538167 None:intergenic
TTAGGAATCGCTTCTAGAGA+TGG 0.528320 8_1:-8538153 Msa1176440:CDS
GATATTCAAGCTGCACAAGA+TGG 0.542476 8_1:-8538066 Msa1176440:CDS
CCAGATGCAAAACACTTACC+TGG 0.548160 8_1:+8539291 None:intergenic
AATGGATGATAGGCAATCAT+GGG 0.552039 8_1:-8538827 Msa1176440:CDS
AAGTCGATTTCATCGTTGGA+TGG 0.552935 8_1:-8539051 Msa1176440:CDS
AATGCAGCCGAGCTAGCAAA+CGG 0.553113 8_1:-8538579 Msa1176440:intron
CCGAGCTAGCAAACGGTGTG+AGG 0.554145 8_1:-8538572 Msa1176440:CDS
TGTGCCGAATATTTCCAAGA+TGG 0.560772 8_1:-8538262 Msa1176440:CDS
ATTGGACCGTGTGACCGATA+CGG 0.561637 8_1:-8539367 Msa1176440:CDS
CTTGGCCTCCTATCATCGGA+GGG 0.566577 8_1:+8538396 None:intergenic
GCAGAAAGCCAGCAAATGGA+AGG 0.566807 8_1:-8538365 Msa1176440:CDS
CCTTGGCCTCCTATCATCGG+AGG 0.567260 8_1:+8538395 None:intergenic
TAATAATCATAGTAGAGTTG+AGG 0.568424 8_1:-8538926 Msa1176440:CDS
TTCTAGAGATGGATCATCAT+CGG 0.575066 8_1:-8538142 Msa1176440:CDS
TTGAGGATGAAGATTGAAGT+GGG 0.584907 8_1:-8539807 None:intergenic
CTAGTCCCTCCGATGATAGG+AGG 0.585538 8_1:-8538401 Msa1176440:CDS
TTTCGGGCAGGATTTGAACT+CGG 0.588089 8_1:-8538884 Msa1176440:CDS
ATGGTGTTCAGGGTTCAAGT+TGG 0.593722 8_1:-8538768 Msa1176440:CDS
TCTGCCTCCACATGATTGAG+GGG 0.599597 8_1:+8538336 None:intergenic
ACAAGATGGAGTTATGGTAA+AGG 0.601453 8_1:-8538052 Msa1176440:CDS
GTGTTGTAGGGAACCTAAAG+AGG 0.602831 8_1:-8539520 Msa1176440:CDS
TAATTCCATTCCCATTCGGA+CGG 0.603735 8_1:+8538800 None:intergenic
AAGAAAGAACATCTCCGTAT+CGG 0.609322 8_1:+8539353 None:intergenic
TGAATTGGGTTCTGTTAGAA+CGG 0.609474 8_1:-8539145 Msa1176440:CDS
TCAACAACACCTAAATCAGT+AGG 0.611590 8_1:+8539165 None:intergenic
CCTCCGATGATAGGAGGCCA+AGG 0.615234 8_1:-8538395 Msa1176440:CDS
AGGTTCCGAGCAGCTGACAA+AGG 0.619745 8_1:-8537896 Msa1176440:CDS
GTTCAGGGTTCAAGTTGGAG+AGG 0.625845 8_1:-8538763 Msa1176440:intron
CCAACAATTGATCTCACTGA+AGG 0.629626 8_1:+8538471 None:intergenic
TCAAGCAAAACTCAAGACGA+TGG 0.633732 8_1:-8538193 Msa1176440:CDS
AAGCTCGTTGATGTAAGCAA+TGG 0.643072 8_1:+8538215 None:intergenic
AAGTTTCTCCCGAAAATGTG+TGG 0.644313 8_1:+8538861 None:intergenic
GGTAGAACCCCTCAATCATG+TGG 0.644553 8_1:-8538343 Msa1176440:CDS
GTGACTTAATTATGAATGTG+TGG 0.646250 8_1:+8537920 None:intergenic
AGGAAAAGGAACAGAATTTG+GGG 0.646477 8_1:-8539497 Msa1176440:CDS
ACATCTCCGTATCGGTCACA+CGG 0.649933 8_1:+8539361 None:intergenic
GATAGGAGGCCAAGGAAACG+TGG 0.651587 8_1:-8538387 Msa1176440:CDS
TTTGAGGATGAAGATTGAAG+TGG 0.652782 8_1:-8539808 None:intergenic
TCAACAACTATCAACAGCAA+CGG 0.665554 8_1:-8538536 Msa1176440:CDS
TTGTAGGGAACCTAAAGAGG+AGG 0.672145 8_1:-8539517 Msa1176440:CDS
TGTTGAAGCTTCTTGCAGGG+AGG 0.673213 8_1:-8538430 Msa1176440:CDS
TCAATCATGTGGAGGCAGAG+AGG 0.677489 8_1:-8538332 Msa1176440:CDS
AGAACCCCTCAATCATGTGG+AGG 0.677611 8_1:-8538340 Msa1176440:CDS
GATGATAGGCAATCATGGGG+AGG 0.677666 8_1:-8538823 Msa1176440:CDS
CAGAAAGCCAGCAAATGGAA+GGG 0.688128 8_1:-8538364 Msa1176440:CDS
ACATTCATAATTAAGTCACA+AGG 0.691255 8_1:-8537916 Msa1176440:CDS
TCTCACTGAAGGTCTCGATG+TGG 0.695158 8_1:+8538482 None:intergenic
ATGGATGATAGGCAATCATG+GGG 0.703008 8_1:-8538826 Msa1176440:CDS
ATAATCATAGTAGAGTTGAG+GGG 0.723398 8_1:-8538924 Msa1176440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAACAATAGTAATAATAA+TGG - chr8_1:8538677-8538696 Msa1176440:intron 15.0%
!! AAGAACAATAGTAATAATAA+TGG - chr8_1:8538677-8538696 Msa1176440:intron 15.0%
!!! TGTTAATGCTTTTGATTATT+TGG - chr8_1:8537929-8537948 Msa1176440:CDS 20.0%
!!! TGTTAATGCTTTTGATTATT+TGG - chr8_1:8537929-8537948 Msa1176440:CDS 20.0%
! ATGAAAAACAACAAAGAATG+AGG - chr8_1:8538198-8538217 Msa1176440:CDS 25.0%
!! ATGTATTTCTCTTTTCCTAA+TGG - chr8_1:8538329-8538348 Msa1176440:CDS 25.0%
!!! CTTTTTCTTGTTTGAAAGTT+GGG - chr8_1:8538645-8538664 Msa1176440:intron 25.0%
!!! TTTTTCTTGTTTGAAAGTTG+GGG - chr8_1:8538646-8538665 Msa1176440:intron 25.0%
! TAATAATCATAGTAGAGTTG+AGG - chr8_1:8538727-8538746 Msa1176440:intron 25.0%
! AATAATCATAGTAGAGTTGA+GGG - chr8_1:8538728-8538747 Msa1176440:intron 25.0%
!!! AATGGAATTAGTTTTCCAAA+TGG - chr8_1:8538866-8538885 Msa1176440:CDS 25.0%
! GAAAAATCAATTTGCATTTG+TGG + chr8_1:8539146-8539165 None:intergenic 25.0%
!!! CAAATGCAAATTGATTTTTC+AGG - chr8_1:8539146-8539165 Msa1176440:CDS 25.0%
! ACATTCATAATTAAGTCACA+AGG - chr8_1:8539737-8539756 Msa1176440:CDS 25.0%
! ATGAAAAACAACAAAGAATG+AGG - chr8_1:8538198-8538217 Msa1176440:CDS 25.0%
!! ATGTATTTCTCTTTTCCTAA+TGG - chr8_1:8538329-8538348 Msa1176440:CDS 25.0%
!!! CTTTTTCTTGTTTGAAAGTT+GGG - chr8_1:8538645-8538664 Msa1176440:intron 25.0%
!!! TTTTTCTTGTTTGAAAGTTG+GGG - chr8_1:8538646-8538665 Msa1176440:intron 25.0%
! TAATAATCATAGTAGAGTTG+AGG - chr8_1:8538727-8538746 Msa1176440:intron 25.0%
! AATAATCATAGTAGAGTTGA+GGG - chr8_1:8538728-8538747 Msa1176440:intron 25.0%
!!! AATGGAATTAGTTTTCCAAA+TGG - chr8_1:8538866-8538885 Msa1176440:CDS 25.0%
! GAAAAATCAATTTGCATTTG+TGG + chr8_1:8539146-8539165 None:intergenic 25.0%
!!! CAAATGCAAATTGATTTTTC+AGG - chr8_1:8539146-8539165 Msa1176440:CDS 25.0%
! ACATTCATAATTAAGTCACA+AGG - chr8_1:8539737-8539756 Msa1176440:CDS 25.0%
GAATGAAGATGAAAAAGCTA+TGG - chr8_1:8537890-8537909 Msa1176440:CDS 30.0%
! AGAATCTTTTGACAGCAATT+GGG - chr8_1:8537973-8537992 Msa1176440:CDS 30.0%
!!! GTTGGAATTATGCGATTTTT+TGG - chr8_1:8538060-8538079 Msa1176440:CDS 30.0%
! GATGATGACAATTATGCTTT+TGG - chr8_1:8538263-8538282 Msa1176440:CDS 30.0%
! TGACAATTATGCTTTTGGAT+TGG - chr8_1:8538268-8538287 Msa1176440:CDS 30.0%
!! TAGCTCTGATTATTGTGTTA+GGG - chr8_1:8538421-8538440 Msa1176440:CDS 30.0%
!!! TTATTGTGTTAGGGCTTTTT+TGG - chr8_1:8538430-8538449 Msa1176440:CDS 30.0%
!!! GTTTTAGTTCCTACTGATTT+AGG - chr8_1:8538479-8538498 Msa1176440:CDS 30.0%
!! TGATTTAGGTGTTGTTGAAT+TGG - chr8_1:8538493-8538512 Msa1176440:CDS 30.0%
!! GATTTAGGTGTTGTTGAATT+GGG - chr8_1:8538494-8538513 Msa1176440:CDS 30.0%
CCAACTTTCAAACAAGAAAA+AGG + chr8_1:8538647-8538666 None:intergenic 30.0%
!! CCTTTTTCTTGTTTGAAAGT+TGG - chr8_1:8538644-8538663 Msa1176440:intron 30.0%
ATAATCATAGTAGAGTTGAG+GGG - chr8_1:8538729-8538748 Msa1176440:intron 30.0%
!! GAAACTTGCTATTAGGAAAA+TGG - chr8_1:8538808-8538827 Msa1176440:CDS 30.0%
CTATTAGGAAAATGGATGAT+AGG - chr8_1:8538816-8538835 Msa1176440:CDS 30.0%
AAACTAATTCCATTCCCATT+CGG + chr8_1:8538860-8538879 None:intergenic 30.0%
TTGTCCATCTTGGAAATATT+CGG + chr8_1:8539398-8539417 None:intergenic 30.0%
GTGACTTAATTATGAATGTG+TGG + chr8_1:8539736-8539755 None:intergenic 30.0%
GAATGAAGATGAAAAAGCTA+TGG - chr8_1:8537890-8537909 Msa1176440:CDS 30.0%
! AGAATCTTTTGACAGCAATT+GGG - chr8_1:8537973-8537992 Msa1176440:CDS 30.0%
!!! GTTGGAATTATGCGATTTTT+TGG - chr8_1:8538060-8538079 Msa1176440:CDS 30.0%
! GATGATGACAATTATGCTTT+TGG - chr8_1:8538263-8538282 Msa1176440:CDS 30.0%
! TGACAATTATGCTTTTGGAT+TGG - chr8_1:8538268-8538287 Msa1176440:CDS 30.0%
!! TAGCTCTGATTATTGTGTTA+GGG - chr8_1:8538421-8538440 Msa1176440:CDS 30.0%
!!! TTATTGTGTTAGGGCTTTTT+TGG - chr8_1:8538430-8538449 Msa1176440:CDS 30.0%
!!! GTTTTAGTTCCTACTGATTT+AGG - chr8_1:8538479-8538498 Msa1176440:CDS 30.0%
!! TGATTTAGGTGTTGTTGAAT+TGG - chr8_1:8538493-8538512 Msa1176440:CDS 30.0%
!! GATTTAGGTGTTGTTGAATT+GGG - chr8_1:8538494-8538513 Msa1176440:CDS 30.0%
CCAACTTTCAAACAAGAAAA+AGG + chr8_1:8538647-8538666 None:intergenic 30.0%
!! CCTTTTTCTTGTTTGAAAGT+TGG - chr8_1:8538644-8538663 Msa1176440:intron 30.0%
ATAATCATAGTAGAGTTGAG+GGG - chr8_1:8538729-8538748 Msa1176440:intron 30.0%
!! GAAACTTGCTATTAGGAAAA+TGG - chr8_1:8538808-8538827 Msa1176440:CDS 30.0%
CTATTAGGAAAATGGATGAT+AGG - chr8_1:8538816-8538835 Msa1176440:CDS 30.0%
AAACTAATTCCATTCCCATT+CGG + chr8_1:8538860-8538879 None:intergenic 30.0%
TTGTCCATCTTGGAAATATT+CGG + chr8_1:8539398-8539417 None:intergenic 30.0%
GTGACTTAATTATGAATGTG+TGG + chr8_1:8539736-8539755 None:intergenic 30.0%
! ATGAAGATTGAAGTGGGTTT+AGG - chr8_1:8537852-8537871 Msa1176440:CDS 35.0%
GTTTAGGAGGAAATAGGTTA+TGG - chr8_1:8537868-8537887 Msa1176440:CDS 35.0%
TGAAGATGAAAAAGCTATGG+TGG - chr8_1:8537893-8537912 Msa1176440:CDS 35.0%
! GAGAATCTTTTGACAGCAAT+TGG - chr8_1:8537972-8537991 Msa1176440:CDS 35.0%
CTAACGTTTCGAATTTCAGT+TGG - chr8_1:8538042-8538061 Msa1176440:CDS 35.0%
CGAATTTCTCAGTCAAAGTT+TGG - chr8_1:8538083-8538102 Msa1176440:CDS 35.0%
! TCAGTCAAAGTTTGGTGATT+GGG - chr8_1:8538091-8538110 Msa1176440:CDS 35.0%
!! AAGTTTGGTGATTGGGTTTT+AGG - chr8_1:8538098-8538117 Msa1176440:CDS 35.0%
GAGGAAAAGGAACAGAATTT+GGG - chr8_1:8538155-8538174 Msa1176440:CDS 35.0%
AGGAAAAGGAACAGAATTTG+GGG - chr8_1:8538156-8538175 Msa1176440:CDS 35.0%
AAGAAAGAACATCTCCGTAT+CGG + chr8_1:8538303-8538322 None:intergenic 35.0%
!! TTCTCTTTTCCTAATGGACA+TGG - chr8_1:8538335-8538354 Msa1176440:CDS 35.0%
!! GTAGCTCTGATTATTGTGTT+AGG - chr8_1:8538420-8538439 Msa1176440:CDS 35.0%
!!! GCTTTTTTGGCTAAATCTGT+TGG - chr8_1:8538443-8538462 Msa1176440:CDS 35.0%
TCAACAACACCTAAATCAGT+AGG + chr8_1:8538491-8538510 None:intergenic 35.0%
TGAATTGGGTTCTGTTAGAA+CGG - chr8_1:8538508-8538527 Msa1176440:CDS 35.0%
! AACACAATCATCGTTTGCTA+GGG - chr8_1:8538577-8538596 Msa1176440:intron 35.0%
! ATTGGAGTTCCCAAGATTTT+CGG - chr8_1:8538752-8538771 Msa1176440:intron 35.0%
AAATGGATGATAGGCAATCA+TGG - chr8_1:8538825-8538844 Msa1176440:CDS 35.0%
AATGGATGATAGGCAATCAT+GGG - chr8_1:8538826-8538845 Msa1176440:CDS 35.0%
! TAGTTTTCCAAATGGTGTTC+AGG - chr8_1:8538874-8538893 Msa1176440:CDS 35.0%
! AGTTTTCCAAATGGTGTTCA+GGG - chr8_1:8538875-8538894 Msa1176440:CDS 35.0%
TCAACAACTATCAACAGCAA+CGG - chr8_1:8539117-8539136 Msa1176440:CDS 35.0%
! GAGATCAATTGTTGGTGAAT+CGG - chr8_1:8539190-8539209 Msa1176440:CDS 35.0%
! ATGGACAAAGCATCTTTGTT+AGG - chr8_1:8539410-8539429 Msa1176440:CDS 35.0%
! TTCTAGAGATGGATCATCAT+CGG - chr8_1:8539511-8539530 Msa1176440:CDS 35.0%
ACAAGATGGAGTTATGGTAA+AGG - chr8_1:8539601-8539620 Msa1176440:CDS 35.0%
!!! GATTGGACGAGTTTTGAAAT+AGG + chr8_1:8539650-8539669 None:intergenic 35.0%
!! TTTCGGTGTCTTTGAATGAT+TGG + chr8_1:8539667-8539686 None:intergenic 35.0%
! CTATAAGGAGTTGGTTTCTT+TGG + chr8_1:8539804-8539823 None:intergenic 35.0%
! ATGAAGATTGAAGTGGGTTT+AGG - chr8_1:8537852-8537871 Msa1176440:CDS 35.0%
GTTTAGGAGGAAATAGGTTA+TGG - chr8_1:8537868-8537887 Msa1176440:CDS 35.0%
TGAAGATGAAAAAGCTATGG+TGG - chr8_1:8537893-8537912 Msa1176440:CDS 35.0%
! GAGAATCTTTTGACAGCAAT+TGG - chr8_1:8537972-8537991 Msa1176440:CDS 35.0%
CTAACGTTTCGAATTTCAGT+TGG - chr8_1:8538042-8538061 Msa1176440:CDS 35.0%
CGAATTTCTCAGTCAAAGTT+TGG - chr8_1:8538083-8538102 Msa1176440:CDS 35.0%
! TCAGTCAAAGTTTGGTGATT+GGG - chr8_1:8538091-8538110 Msa1176440:CDS 35.0%
!! AAGTTTGGTGATTGGGTTTT+AGG - chr8_1:8538098-8538117 Msa1176440:CDS 35.0%
GAGGAAAAGGAACAGAATTT+GGG - chr8_1:8538155-8538174 Msa1176440:CDS 35.0%
AGGAAAAGGAACAGAATTTG+GGG - chr8_1:8538156-8538175 Msa1176440:CDS 35.0%
AAGAAAGAACATCTCCGTAT+CGG + chr8_1:8538303-8538322 None:intergenic 35.0%
!! TTCTCTTTTCCTAATGGACA+TGG - chr8_1:8538335-8538354 Msa1176440:CDS 35.0%
!! GTAGCTCTGATTATTGTGTT+AGG - chr8_1:8538420-8538439 Msa1176440:CDS 35.0%
!!! GCTTTTTTGGCTAAATCTGT+TGG - chr8_1:8538443-8538462 Msa1176440:CDS 35.0%
TCAACAACACCTAAATCAGT+AGG + chr8_1:8538491-8538510 None:intergenic 35.0%
TGAATTGGGTTCTGTTAGAA+CGG - chr8_1:8538508-8538527 Msa1176440:CDS 35.0%
! AACACAATCATCGTTTGCTA+GGG - chr8_1:8538577-8538596 Msa1176440:intron 35.0%
! ATTGGAGTTCCCAAGATTTT+CGG - chr8_1:8538752-8538771 Msa1176440:intron 35.0%
AAATGGATGATAGGCAATCA+TGG - chr8_1:8538825-8538844 Msa1176440:CDS 35.0%
AATGGATGATAGGCAATCAT+GGG - chr8_1:8538826-8538845 Msa1176440:CDS 35.0%
! TAGTTTTCCAAATGGTGTTC+AGG - chr8_1:8538874-8538893 Msa1176440:CDS 35.0%
! AGTTTTCCAAATGGTGTTCA+GGG - chr8_1:8538875-8538894 Msa1176440:CDS 35.0%
TCAACAACTATCAACAGCAA+CGG - chr8_1:8539117-8539136 Msa1176440:CDS 35.0%
! GAGATCAATTGTTGGTGAAT+CGG - chr8_1:8539190-8539209 Msa1176440:CDS 35.0%
! ATGGACAAAGCATCTTTGTT+AGG - chr8_1:8539410-8539429 Msa1176440:CDS 35.0%
! TTCTAGAGATGGATCATCAT+CGG - chr8_1:8539511-8539530 Msa1176440:CDS 35.0%
ACAAGATGGAGTTATGGTAA+AGG - chr8_1:8539601-8539620 Msa1176440:CDS 35.0%
!!! GATTGGACGAGTTTTGAAAT+AGG + chr8_1:8539650-8539669 None:intergenic 35.0%
!! TTTCGGTGTCTTTGAATGAT+TGG + chr8_1:8539667-8539686 None:intergenic 35.0%
! CTATAAGGAGTTGGTTTCTT+TGG + chr8_1:8539804-8539823 None:intergenic 35.0%
! AAGATTGAAGTGGGTTTAGG+AGG - chr8_1:8537855-8537874 Msa1176440:CDS 40.0%
! AAGTGGGTTTAGGAGGAAAT+AGG - chr8_1:8537862-8537881 Msa1176440:CDS 40.0%
! GCTATGGTGGTTGAAGTTTT+AGG - chr8_1:8537906-8537925 Msa1176440:CDS 40.0%
! CTCAGTCAAAGTTTGGTGAT+TGG - chr8_1:8538090-8538109 Msa1176440:CDS 40.0%
!!! TTGGTGATTGGGTTTTAGGT+TGG - chr8_1:8538102-8538121 Msa1176440:CDS 40.0%
!!! TGGTGATTGGGTTTTAGGTT+GGG - chr8_1:8538103-8538122 Msa1176440:CDS 40.0%
! TGTTCCTTTTCCTCCTCTTT+AGG + chr8_1:8538149-8538168 None:intergenic 40.0%
GGAGGAAAAGGAACAGAATT+TGG - chr8_1:8538154-8538173 Msa1176440:CDS 40.0%
!! TCTTTTCCTAATGGACATGG+TGG - chr8_1:8538338-8538357 Msa1176440:CDS 40.0%
! CAGGTAAGTGTTTTGCATCT+GGG - chr8_1:8538363-8538382 Msa1176440:CDS 40.0%
CAACACAATCATCGTTTGCT+AGG - chr8_1:8538576-8538595 Msa1176440:intron 40.0%
! GGTTAAGTCGATTTCATCGT+TGG - chr8_1:8538598-8538617 Msa1176440:intron 40.0%
! AAGTCGATTTCATCGTTGGA+TGG - chr8_1:8538602-8538621 Msa1176440:intron 40.0%
! TTGGAGTTCCCAAGATTTTC+GGG - chr8_1:8538753-8538772 Msa1176440:intron 40.0%
AAATCCTGCCCGAAAATCTT+GGG + chr8_1:8538764-8538783 None:intergenic 40.0%
AAGTTTCTCCCGAAAATGTG+TGG + chr8_1:8538795-8538814 None:intergenic 40.0%
!! TTCGGGAGAAACTTGCTATT+AGG - chr8_1:8538801-8538820 Msa1176440:CDS 40.0%
ATGGATGATAGGCAATCATG+GGG - chr8_1:8538827-8538846 Msa1176440:CDS 40.0%
TAATTCCATTCCCATTCGGA+CGG + chr8_1:8538856-8538875 None:intergenic 40.0%
CCAACAATTGATCTCACTGA+AGG + chr8_1:8539185-8539204 None:intergenic 40.0%
CCTTCAGTGAGATCAATTGT+TGG - chr8_1:8539182-8539201 Msa1176440:CDS 40.0%
TGATGTTGAAGCTTCTTGCA+GGG - chr8_1:8539220-8539239 Msa1176440:CDS 40.0%
TGTGCCGAATATTTCCAAGA+TGG - chr8_1:8539391-8539410 Msa1176440:CDS 40.0%
CAAAGATGCTTTGTCCATCT+TGG + chr8_1:8539408-8539427 None:intergenic 40.0%
AAGCTCGTTGATGTAAGCAA+TGG + chr8_1:8539441-8539460 None:intergenic 40.0%
TCAAGCAAAACTCAAGACGA+TGG - chr8_1:8539460-8539479 Msa1176440:CDS 40.0%
GATTCCTAATTGCTCTCTCT+CGG + chr8_1:8539489-8539508 None:intergenic 40.0%
! TTAGGAATCGCTTCTAGAGA+TGG - chr8_1:8539500-8539519 Msa1176440:CDS 40.0%
!!! TTTTCTGCTCTAGAGTTTGC+TGG + chr8_1:8539542-8539561 None:intergenic 40.0%
GCAGCTTGAATATCGATATC+AGG + chr8_1:8539581-8539600 None:intergenic 40.0%
GATATTCAAGCTGCACAAGA+TGG - chr8_1:8539587-8539606 Msa1176440:CDS 40.0%
!! CGACTCAACGATATCGATTT+CGG + chr8_1:8539684-8539703 None:intergenic 40.0%
! AAGATTGAAGTGGGTTTAGG+AGG - chr8_1:8537855-8537874 Msa1176440:CDS 40.0%
! AAGTGGGTTTAGGAGGAAAT+AGG - chr8_1:8537862-8537881 Msa1176440:CDS 40.0%
! GCTATGGTGGTTGAAGTTTT+AGG - chr8_1:8537906-8537925 Msa1176440:CDS 40.0%
! CTCAGTCAAAGTTTGGTGAT+TGG - chr8_1:8538090-8538109 Msa1176440:CDS 40.0%
!!! TTGGTGATTGGGTTTTAGGT+TGG - chr8_1:8538102-8538121 Msa1176440:CDS 40.0%
!!! TGGTGATTGGGTTTTAGGTT+GGG - chr8_1:8538103-8538122 Msa1176440:CDS 40.0%
! TGTTCCTTTTCCTCCTCTTT+AGG + chr8_1:8538149-8538168 None:intergenic 40.0%
GGAGGAAAAGGAACAGAATT+TGG - chr8_1:8538154-8538173 Msa1176440:CDS 40.0%
!! TCTTTTCCTAATGGACATGG+TGG - chr8_1:8538338-8538357 Msa1176440:CDS 40.0%
! CAGGTAAGTGTTTTGCATCT+GGG - chr8_1:8538363-8538382 Msa1176440:CDS 40.0%
CAACACAATCATCGTTTGCT+AGG - chr8_1:8538576-8538595 Msa1176440:intron 40.0%
! GGTTAAGTCGATTTCATCGT+TGG - chr8_1:8538598-8538617 Msa1176440:intron 40.0%
! AAGTCGATTTCATCGTTGGA+TGG - chr8_1:8538602-8538621 Msa1176440:intron 40.0%
! TTGGAGTTCCCAAGATTTTC+GGG - chr8_1:8538753-8538772 Msa1176440:intron 40.0%
AAATCCTGCCCGAAAATCTT+GGG + chr8_1:8538764-8538783 None:intergenic 40.0%
AAGTTTCTCCCGAAAATGTG+TGG + chr8_1:8538795-8538814 None:intergenic 40.0%
!! TTCGGGAGAAACTTGCTATT+AGG - chr8_1:8538801-8538820 Msa1176440:CDS 40.0%
ATGGATGATAGGCAATCATG+GGG - chr8_1:8538827-8538846 Msa1176440:CDS 40.0%
TAATTCCATTCCCATTCGGA+CGG + chr8_1:8538856-8538875 None:intergenic 40.0%
CCAACAATTGATCTCACTGA+AGG + chr8_1:8539185-8539204 None:intergenic 40.0%
CCTTCAGTGAGATCAATTGT+TGG - chr8_1:8539182-8539201 Msa1176440:CDS 40.0%
TGATGTTGAAGCTTCTTGCA+GGG - chr8_1:8539220-8539239 Msa1176440:CDS 40.0%
TGTGCCGAATATTTCCAAGA+TGG - chr8_1:8539391-8539410 Msa1176440:CDS 40.0%
CAAAGATGCTTTGTCCATCT+TGG + chr8_1:8539408-8539427 None:intergenic 40.0%
AAGCTCGTTGATGTAAGCAA+TGG + chr8_1:8539441-8539460 None:intergenic 40.0%
TCAAGCAAAACTCAAGACGA+TGG - chr8_1:8539460-8539479 Msa1176440:CDS 40.0%
GATTCCTAATTGCTCTCTCT+CGG + chr8_1:8539489-8539508 None:intergenic 40.0%
! TTAGGAATCGCTTCTAGAGA+TGG - chr8_1:8539500-8539519 Msa1176440:CDS 40.0%
!!! TTTTCTGCTCTAGAGTTTGC+TGG + chr8_1:8539542-8539561 None:intergenic 40.0%
GCAGCTTGAATATCGATATC+AGG + chr8_1:8539581-8539600 None:intergenic 40.0%
GATATTCAAGCTGCACAAGA+TGG - chr8_1:8539587-8539606 Msa1176440:CDS 40.0%
!! CGACTCAACGATATCGATTT+CGG + chr8_1:8539684-8539703 None:intergenic 40.0%
! CTTTTGACAGCAATTGGGAG+TGG - chr8_1:8537978-8537997 Msa1176440:CDS 45.0%
!!! GGTGATTGGGTTTTAGGTTG+GGG - chr8_1:8538104-8538123 Msa1176440:CDS 45.0%
GTGTTGTAGGGAACCTAAAG+AGG - chr8_1:8538133-8538152 Msa1176440:CDS 45.0%
TTGTAGGGAACCTAAAGAGG+AGG - chr8_1:8538136-8538155 Msa1176440:CDS 45.0%
GGAACCTAAAGAGGAGGAAA+AGG - chr8_1:8538142-8538161 Msa1176440:CDS 45.0%
CAGAAGCTTCATGCGACATT+TGG - chr8_1:8538233-8538252 Msa1176440:CDS 45.0%
AAGCTTCATGCGACATTTGG+TGG - chr8_1:8538236-8538255 Msa1176440:CDS 45.0%
CCAGATGCAAAACACTTACC+TGG + chr8_1:8538365-8538384 None:intergenic 45.0%
! CCAGGTAAGTGTTTTGCATC+TGG - chr8_1:8538362-8538381 Msa1176440:CDS 45.0%
!! TTGCATCTGGGAAGCATTTG+TGG - chr8_1:8538375-8538394 Msa1176440:CDS 45.0%
TTGGGTTCTGTTAGAACGGT+TGG - chr8_1:8538512-8538531 Msa1176440:CDS 45.0%
CATAGTAGAGTTGAGGGGAT+TGG - chr8_1:8538734-8538753 Msa1176440:intron 45.0%
CAAATCCTGCCCGAAAATCT+TGG + chr8_1:8538765-8538784 None:intergenic 45.0%
TTTCGGGCAGGATTTGAACT+CGG - chr8_1:8538769-8538788 Msa1176440:CDS 45.0%
! TGAACTCGGCCACACATTTT+CGG - chr8_1:8538783-8538802 Msa1176440:CDS 45.0%
CTTGAACCCTGAACACCATT+TGG + chr8_1:8538884-8538903 None:intergenic 45.0%
ATGGTGTTCAGGGTTCAAGT+TGG - chr8_1:8538885-8538904 Msa1176440:CDS 45.0%
CTGATGTTGAAGCTTCTTGC+AGG - chr8_1:8539219-8539238 Msa1176440:CDS 45.0%
TTTCCTTGGCCTCCTATCAT+CGG + chr8_1:8539264-8539283 None:intergenic 45.0%
CAGAAAGCCAGCAAATGGAA+GGG - chr8_1:8539289-8539308 Msa1176440:CDS 45.0%
CTCGTAATGCGTAAAAACGC+TGG + chr8_1:8539367-8539386 None:intergenic 45.0%
GAATCCGAGAGAGAGCAATT+AGG - chr8_1:8539482-8539501 Msa1176440:CDS 45.0%
AGCTGCACAAGATGGAGTTA+TGG - chr8_1:8539595-8539614 Msa1176440:CDS 45.0%
! CTTTTGACAGCAATTGGGAG+TGG - chr8_1:8537978-8537997 Msa1176440:CDS 45.0%
!!! GGTGATTGGGTTTTAGGTTG+GGG - chr8_1:8538104-8538123 Msa1176440:CDS 45.0%
GTGTTGTAGGGAACCTAAAG+AGG - chr8_1:8538133-8538152 Msa1176440:CDS 45.0%
TTGTAGGGAACCTAAAGAGG+AGG - chr8_1:8538136-8538155 Msa1176440:CDS 45.0%
GGAACCTAAAGAGGAGGAAA+AGG - chr8_1:8538142-8538161 Msa1176440:CDS 45.0%
CAGAAGCTTCATGCGACATT+TGG - chr8_1:8538233-8538252 Msa1176440:CDS 45.0%
AAGCTTCATGCGACATTTGG+TGG - chr8_1:8538236-8538255 Msa1176440:CDS 45.0%
CCAGATGCAAAACACTTACC+TGG + chr8_1:8538365-8538384 None:intergenic 45.0%
! CCAGGTAAGTGTTTTGCATC+TGG - chr8_1:8538362-8538381 Msa1176440:CDS 45.0%
!! TTGCATCTGGGAAGCATTTG+TGG - chr8_1:8538375-8538394 Msa1176440:CDS 45.0%
TTGGGTTCTGTTAGAACGGT+TGG - chr8_1:8538512-8538531 Msa1176440:CDS 45.0%
CATAGTAGAGTTGAGGGGAT+TGG - chr8_1:8538734-8538753 Msa1176440:intron 45.0%
CAAATCCTGCCCGAAAATCT+TGG + chr8_1:8538765-8538784 None:intergenic 45.0%
TTTCGGGCAGGATTTGAACT+CGG - chr8_1:8538769-8538788 Msa1176440:CDS 45.0%
! TGAACTCGGCCACACATTTT+CGG - chr8_1:8538783-8538802 Msa1176440:CDS 45.0%
CTTGAACCCTGAACACCATT+TGG + chr8_1:8538884-8538903 None:intergenic 45.0%
ATGGTGTTCAGGGTTCAAGT+TGG - chr8_1:8538885-8538904 Msa1176440:CDS 45.0%
CTGATGTTGAAGCTTCTTGC+AGG - chr8_1:8539219-8539238 Msa1176440:CDS 45.0%
TTTCCTTGGCCTCCTATCAT+CGG + chr8_1:8539264-8539283 None:intergenic 45.0%
CAGAAAGCCAGCAAATGGAA+GGG - chr8_1:8539289-8539308 Msa1176440:CDS 45.0%
CTCGTAATGCGTAAAAACGC+TGG + chr8_1:8539367-8539386 None:intergenic 45.0%
GAATCCGAGAGAGAGCAATT+AGG - chr8_1:8539482-8539501 Msa1176440:CDS 45.0%
AGCTGCACAAGATGGAGTTA+TGG - chr8_1:8539595-8539614 Msa1176440:CDS 45.0%
!!! TGGGTTTTAGGTTGGGGAGA+TGG - chr8_1:8538110-8538129 Msa1176440:CDS 50.0%
!!! GGGTTTTAGGTTGGGGAGAT+GGG - chr8_1:8538111-8538130 Msa1176440:CDS 50.0%
! TTGGGGAGATGGGTGTTGTA+GGG - chr8_1:8538121-8538140 Msa1176440:CDS 50.0%
ATTGGACCGTGTGACCGATA+CGG - chr8_1:8538286-8538305 Msa1176440:CDS 50.0%
! ACATCTCCGTATCGGTCACA+CGG + chr8_1:8538295-8538314 None:intergenic 50.0%
!! AGTTCCCAAGATTTTCGGGC+AGG - chr8_1:8538757-8538776 Msa1176440:intron 50.0%
! GAACTCGGCCACACATTTTC+GGG - chr8_1:8538784-8538803 Msa1176440:CDS 50.0%
GATGATAGGCAATCATGGGG+AGG - chr8_1:8538830-8538849 Msa1176440:CDS 50.0%
!! GTTCAGGGTTCAAGTTGGAG+AGG - chr8_1:8538890-8538909 Msa1176440:CDS 50.0%
! GGAGAGGTAGTCAAGTTGTG+AGG - chr8_1:8538906-8538925 Msa1176440:CDS 50.0%
AATGCAGCCGAGCTAGCAAA+CGG - chr8_1:8539074-8539093 Msa1176440:CDS 50.0%
TCTCACTGAAGGTCTCGATG+TGG + chr8_1:8539174-8539193 None:intergenic 50.0%
TGTTGAAGCTTCTTGCAGGG+AGG - chr8_1:8539223-8539242 Msa1176440:CDS 50.0%
ATCCTAGTCCCTCCGATGAT+AGG - chr8_1:8539249-8539268 Msa1176440:CDS 50.0%
GCAGAAAGCCAGCAAATGGA+AGG - chr8_1:8539288-8539307 Msa1176440:CDS 50.0%
GGTTCTACCCTTCCATTTGC+TGG + chr8_1:8539299-8539318 None:intergenic 50.0%
GGTAGAACCCCTCAATCATG+TGG - chr8_1:8539310-8539329 Msa1176440:CDS 50.0%
AGAACCCCTCAATCATGTGG+AGG - chr8_1:8539313-8539332 Msa1176440:CDS 50.0%
TCTGCCTCCACATGATTGAG+GGG + chr8_1:8539320-8539339 None:intergenic 50.0%
CTCTGCCTCCACATGATTGA+GGG + chr8_1:8539321-8539340 None:intergenic 50.0%
TCTCTGCCTCCACATGATTG+AGG + chr8_1:8539322-8539341 None:intergenic 50.0%
TCAATCATGTGGAGGCAGAG+AGG - chr8_1:8539321-8539340 Msa1176440:CDS 50.0%
CTTCTCCTTTGTCAGCTGCT+CGG + chr8_1:8539765-8539784 None:intergenic 50.0%
!!! TGGGTTTTAGGTTGGGGAGA+TGG - chr8_1:8538110-8538129 Msa1176440:CDS 50.0%
!!! GGGTTTTAGGTTGGGGAGAT+GGG - chr8_1:8538111-8538130 Msa1176440:CDS 50.0%
! TTGGGGAGATGGGTGTTGTA+GGG - chr8_1:8538121-8538140 Msa1176440:CDS 50.0%
ATTGGACCGTGTGACCGATA+CGG - chr8_1:8538286-8538305 Msa1176440:CDS 50.0%
! ACATCTCCGTATCGGTCACA+CGG + chr8_1:8538295-8538314 None:intergenic 50.0%
!! AGTTCCCAAGATTTTCGGGC+AGG - chr8_1:8538757-8538776 Msa1176440:intron 50.0%
! GAACTCGGCCACACATTTTC+GGG - chr8_1:8538784-8538803 Msa1176440:CDS 50.0%
GATGATAGGCAATCATGGGG+AGG - chr8_1:8538830-8538849 Msa1176440:CDS 50.0%
!! GTTCAGGGTTCAAGTTGGAG+AGG - chr8_1:8538890-8538909 Msa1176440:CDS 50.0%
! GGAGAGGTAGTCAAGTTGTG+AGG - chr8_1:8538906-8538925 Msa1176440:CDS 50.0%
AATGCAGCCGAGCTAGCAAA+CGG - chr8_1:8539074-8539093 Msa1176440:CDS 50.0%
TCTCACTGAAGGTCTCGATG+TGG + chr8_1:8539174-8539193 None:intergenic 50.0%
TGTTGAAGCTTCTTGCAGGG+AGG - chr8_1:8539223-8539242 Msa1176440:CDS 50.0%
ATCCTAGTCCCTCCGATGAT+AGG - chr8_1:8539249-8539268 Msa1176440:CDS 50.0%
GCAGAAAGCCAGCAAATGGA+AGG - chr8_1:8539288-8539307 Msa1176440:CDS 50.0%
GGTTCTACCCTTCCATTTGC+TGG + chr8_1:8539299-8539318 None:intergenic 50.0%
GGTAGAACCCCTCAATCATG+TGG - chr8_1:8539310-8539329 Msa1176440:CDS 50.0%
AGAACCCCTCAATCATGTGG+AGG - chr8_1:8539313-8539332 Msa1176440:CDS 50.0%
TCTGCCTCCACATGATTGAG+GGG + chr8_1:8539320-8539339 None:intergenic 50.0%
CTCTGCCTCCACATGATTGA+GGG + chr8_1:8539321-8539340 None:intergenic 50.0%
TCTCTGCCTCCACATGATTG+AGG + chr8_1:8539322-8539341 None:intergenic 50.0%
TCAATCATGTGGAGGCAGAG+AGG - chr8_1:8539321-8539340 Msa1176440:CDS 50.0%
CTTCTCCTTTGTCAGCTGCT+CGG + chr8_1:8539765-8539784 None:intergenic 50.0%
! GTTGGGGAGATGGGTGTTGT+AGG - chr8_1:8538120-8538139 Msa1176440:CDS 55.0%
CCTGGTCCACCATGTCCATT+AGG + chr8_1:8538347-8538366 None:intergenic 55.0%
!! CCTAATGGACATGGTGGACC+AGG - chr8_1:8538344-8538363 Msa1176440:CDS 55.0%
GCATTGCTCGATGAAGGCCT+AGG + chr8_1:8539059-8539078 None:intergenic 55.0%
TCGGCTGCATTGCTCGATGA+AGG + chr8_1:8539065-8539084 None:intergenic 55.0%
! CCTCACACCGTTTGCTAGCT+CGG + chr8_1:8539084-8539103 None:intergenic 55.0%
CTCCTATCATCGGAGGGACT+AGG + chr8_1:8539254-8539273 None:intergenic 55.0%
CTAGTCCCTCCGATGATAGG+AGG - chr8_1:8539252-8539271 Msa1176440:CDS 55.0%
CTTGGCCTCCTATCATCGGA+GGG + chr8_1:8539260-8539279 None:intergenic 55.0%
GATAGGAGGCCAAGGAAACG+TGG - chr8_1:8539266-8539285 Msa1176440:CDS 55.0%
GGCTTTCTGCCACGTTTCCT+TGG + chr8_1:8539278-8539297 None:intergenic 55.0%
CGTGGCAGAAAGCCAGCAAA+TGG - chr8_1:8539284-8539303 Msa1176440:CDS 55.0%
AGGTTCCGAGCAGCTGACAA+AGG - chr8_1:8539757-8539776 Msa1176440:CDS 55.0%
! GTTGGGGAGATGGGTGTTGT+AGG - chr8_1:8538120-8538139 Msa1176440:CDS 55.0%
CCTGGTCCACCATGTCCATT+AGG + chr8_1:8538347-8538366 None:intergenic 55.0%
!! CCTAATGGACATGGTGGACC+AGG - chr8_1:8538344-8538363 Msa1176440:CDS 55.0%
GCATTGCTCGATGAAGGCCT+AGG + chr8_1:8539059-8539078 None:intergenic 55.0%
TCGGCTGCATTGCTCGATGA+AGG + chr8_1:8539065-8539084 None:intergenic 55.0%
! CCTCACACCGTTTGCTAGCT+CGG + chr8_1:8539084-8539103 None:intergenic 55.0%
CTCCTATCATCGGAGGGACT+AGG + chr8_1:8539254-8539273 None:intergenic 55.0%
CTAGTCCCTCCGATGATAGG+AGG - chr8_1:8539252-8539271 Msa1176440:CDS 55.0%
CTTGGCCTCCTATCATCGGA+GGG + chr8_1:8539260-8539279 None:intergenic 55.0%
GATAGGAGGCCAAGGAAACG+TGG - chr8_1:8539266-8539285 Msa1176440:CDS 55.0%
GGCTTTCTGCCACGTTTCCT+TGG + chr8_1:8539278-8539297 None:intergenic 55.0%
CGTGGCAGAAAGCCAGCAAA+TGG - chr8_1:8539284-8539303 Msa1176440:CDS 55.0%
AGGTTCCGAGCAGCTGACAA+AGG - chr8_1:8539757-8539776 Msa1176440:CDS 55.0%
CCGAGCTAGCAAACGGTGTG+AGG - chr8_1:8539081-8539100 Msa1176440:CDS 60.0%
CCTTGGCCTCCTATCATCGG+AGG + chr8_1:8539261-8539280 None:intergenic 60.0%
CCTCCGATGATAGGAGGCCA+AGG - chr8_1:8539258-8539277 Msa1176440:CDS 60.0%
CCGAGCTAGCAAACGGTGTG+AGG - chr8_1:8539081-8539100 Msa1176440:CDS 60.0%
CCTTGGCCTCCTATCATCGG+AGG + chr8_1:8539261-8539280 None:intergenic 60.0%
CCTCCGATGATAGGAGGCCA+AGG - chr8_1:8539258-8539277 Msa1176440:CDS 60.0%
TCATGGGGAGGCCGTCCGAA+TGG - chr8_1:8538842-8538861 Msa1176440:CDS 65.0%
CATGGGGAGGCCGTCCGAAT+GGG - chr8_1:8538843-8538862 Msa1176440:CDS 65.0%
GGAGGCCGTCCGAATGGGAA+TGG - chr8_1:8538848-8538867 Msa1176440:CDS 65.0%
TCATGGGGAGGCCGTCCGAA+TGG - chr8_1:8538842-8538861 Msa1176440:CDS 65.0%
CATGGGGAGGCCGTCCGAAT+GGG - chr8_1:8538843-8538862 Msa1176440:CDS 65.0%
GGAGGCCGTCCGAATGGGAA+TGG - chr8_1:8538848-8538867 Msa1176440:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 8537852 8539823 8537852 ID=Msa1176440;Name=Msa1176440
chr8_1 mRNA 8537852 8539823 8537852 ID=Msa1176440-mRNA-1;Parent=Msa1176440;Name=Msa1176440-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|600
chr8_1 exon 8538764 8539823 8538764 ID=Msa1176440-mRNA-1:exon:2904;Parent=Msa1176440-mRNA-1
chr8_1 exon 8537852 8538594 8537852 ID=Msa1176440-mRNA-1:exon:2903;Parent=Msa1176440-mRNA-1
chr8_1 CDS 8538764 8539823 8538764 ID=Msa1176440-mRNA-1:cds;Parent=Msa1176440-mRNA-1
chr8_1 CDS 8537852 8538594 8537852 ID=Msa1176440-mRNA-1:cds;Parent=Msa1176440-mRNA-1
Gene Sequence

>Msa1176440

ATGAAGATTGAAGTGGGTTTAGGAGGAAATAGGTTATGGAATGAAGATGAAAAAGCTATGGTGGTTGAAGTTTTAGGTGTTAATGCTTTTGATTATTTGGTTACAAATTCAGTTTCAAATGAGAATCTTTTGACAGCAATTGGGAGTGGTGAGAATCTTGAAAACAAGCTTTCTGATCTAGTTGAAAGATCTAACGTTTCGAATTTCAGTTGGAATTATGCGATTTTTTGGCGAATTTCTCAGTCAAAGTTTGGTGATTGGGTTTTAGGTTGGGGAGATGGGTGTTGTAGGGAACCTAAAGAGGAGGAAAAGGAACAGAATTTGGGGATGAAAAGTGTTGTTGAAGATGAAAAACAACAAAGAATGAGGAAAAGTGTGTTGCAGAAGCTTCATGCGACATTTGGTGGTTTAGATGATGACAATTATGCTTTTGGATTGGACCGTGTGACCGATACGGAGATGTTCTTTCTTGCATCAATGTATTTCTCTTTTCCTAATGGACATGGTGGACCAGGTAAGTGTTTTGCATCTGGGAAGCATTTGTGGTTATGTGATGCATTGAAATCTAGTAGCTCTGATTATTGTGTTAGGGCTTTTTTGGCTAAATCTGTTGGATTTAAGACTATTGTTTTAGTTCCTACTGATTTAGGTGTTGTTGAATTGGGTTCTGTTAGAACGGTTGGCGAGAGCGTTGAATTGTTGCAAACTATTAAATCTGTGTTTTCAACACAATCATCGTTTGCTAGGGTTAAGTCGATTTCATCGTTGGATGGAAAGAGAGACGAGAATGCTCCTTTTTCTTGTTTGAAAGTTGGGGAGAGTGTTAAGAACAATAGTAATAATAATGGCAATCTTCATCTTCATCATCAGAATAATAATAATCATAGTAGAGTTGAGGGGATTGGAGTTCCCAAGATTTTCGGGCAGGATTTGAACTCGGCCACACATTTTCGGGAGAAACTTGCTATTAGGAAAATGGATGATAGGCAATCATGGGGAGGCCGTCCGAATGGGAATGGAATTAGTTTTCCAAATGGTGTTCAGGGTTCAAGTTGGAGAGCCGAGCTAGCAAACGGTGTGAGGCAAGATTTCGTGCTCAACAACTATCAACAGCAACGGAAGCCACAAATGCAAATTGATTTTTCAGGAGCCACATCGAGACCTTCAGTGAGATCAATTGTTGGTGAATCGGAGCTTTCTGATGTTGAAGCTTCTTGCAGGGAGGAGAATCCTAGTCCCTCCGATGATAGGAGGCCAAGGAAACGTGGCAGAAAGCCAGCAAATGGAAGGGTAGAACCCCTCAATCATGTGGAGGCAGAGAGGCAAAGACGCGAGAAGCTAAACCAGCGTTTTTACGCATTACGAGCTGTTGTGCCGAATATTTCCAAGATGGACAAAGCATCTTTGTTAGGAGATGCCATTGCTTACATCAACGAGCTTCAAGCAAAACTCAAGACGATGGAATCCGAGAGAGAGCAATTAGGAATCGCTTCTAGAGATGGATCATCATCGGTATTGCCAGCAAACTCTAGAGCAGAAAATCATCAAAGCAGTGCTCCTGATATCGATATTCAAGCTGCACAAGATGGAGTTATGGTAAAGGTGAGTTGTTCTATTGATGTTCATCCTATTTCAAAACTCGTCCAATCATTCAAAGACACCGAAATCGATATCGTTGAGTCGAAACTCAATGCTGCAAATGATACTGTCTTCCACACATTCATAATTAAGTCACAAGGTTCCGAGCAGCTGACAAAGGAGAAGTTGATTGCAGCATTTTCCAAAGAAACCAACTCCTTATAG
Protein sequence

>Msa1176440

MKIEVGLGGNRLWNEDEKAMVVEVLGVNAFDYLVTNSVSNENLLTAIGSGENLENKLSDLVERSNVSNFSWNYAIFWRISQSKFGDWVLGWGDGCCREPKEEEKEQNLGMKSVVEDEKQQRMRKSVLQKLHATFGGLDDDNYAFGLDRVTDTEMFFLASMYFSFPNGHGGPGKCFASGKHLWLCDALKSSSSDYCVRAFLAKSVGFKTIVLVPTDLGVVELGSVRTVGESVELLQTIKSVFSTQSSFARVKSISSLDGKRDENAPFSCLKVGESVKNNSNNNGNLHLHHQNNNNHSRVEGIGVPKIFGQDLNSATHFREKLAIRKMDDRQSWGGRPNGNGISFPNGVQGSSWRAELANGVRQDFVLNNYQQQRKPQMQIDFSGATSRPSVRSIVGESELSDVEASCREENPSPSDDRRPRKRGRKPANGRVEPLNHVEAERQRREKLNQRFYALRAVVPNISKMDKASLLGDAIAYINELQAKLKTMESEREQLGIASRDGSSSVLPANSRAENHQSSAPDIDIQAAQDGVMVKVSCSIDVHPISKLVQSFKDTEIDIVESKLNAANDTVFHTFIIKSQGSEQLTKEKLIAAFSKETNSL*